Gene Symbol: AFF1
Description: AF4/FMR2 family member 1
Alias: AF4, MLLT2, PBM1, AF4/FMR2 family member 1, ALL1-fused gene from chromosome 4 protein, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 2, pre-B-cell monocytic leukemia partner 1, proto-oncogene AF4
Species: human
Products:     AFF1

Top Publications

  1. Yokoyama A, Lin M, Naresh A, Kitabayashi I, Cleary M. A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. Cancer Cell. 2010;17:198-212 pubmed publisher
    b>AF4 and ENL family proteins are frequently fused with MLL, and they comprise a higher order complex (designated AEP) containing the P-TEFb transcription elongation factor...
  2. Xia Z, Popovic R, Chen J, Theisler C, Stuart T, Santillan D, et al. The MLL fusion gene, MLL-AF4, regulates cyclin-dependent kinase inhibitor CDKN1B (p27kip1) expression. Proc Natl Acad Sci U S A. 2005;102:14028-33 pubmed
    ..We explored downstream target genes of the most prevalent MLL fusion protein, MLL-AF4. To this end, we developed inducible MLL-AF4 fusion cell lines in different backgrounds...
  3. Krivtsov A, Feng Z, Lemieux M, Faber J, Vempati S, Sinha A, et al. H3K79 methylation profiles define murine and human MLL-AF4 leukemias. Cancer Cell. 2008;14:355-68 pubmed publisher
    We created a mouse model wherein conditional expression of an Mll-AF4 fusion oncogene induces B precursor acute lymphoblastic (ALL) or acute myeloid leukemias (AML)...
  4. Lu H, Li Z, Xue Y, Schulze Gahmen U, Johnson J, Krogan N, et al. AFF1 is a ubiquitous P-TEFb partner to enable Tat extraction of P-TEFb from 7SK snRNP and formation of SECs for HIV transactivation. Proc Natl Acad Sci U S A. 2014;111:E15-24 pubmed publisher
    ..Here we show that the AF4/FMR2 family member 1 (AFF1) is bound to CDK9-CycT and is present in all major P-TEFb complexes and that the tripartite CDK9-CycT-AFF1 complex ..
  5. Erfurth F, Hemenway C, de Erkenez A, Domer P. MLL fusion partners AF4 and AF9 interact at subnuclear foci. Leukemia. 2004;18:92-102 pubmed
    ..We have identified a protein interaction between the two most common MLL fusion partners AF4 and AF9. This interaction is restricted to discrete nuclear foci we have named 'AF4 bodies'...
  6. Zeisig D, Bittner C, Zeisig B, Garcia Cuellar M, Hess J, Slany R. The eleven-nineteen-leukemia protein ENL connects nuclear MLL fusion partners with chromatin. Oncogene. 2005;24:5525-32 pubmed
    ..In two-hybrid studies, ENL interacted with AF4 and AF5q31 as well as with a fragment of AF10...
  7. Sobhian B, Laguette N, Yatim A, Nakamura M, Levy Y, Kiernan R, et al. HIV-1 Tat assembles a multifunctional transcription elongation complex and stably associates with the 7SK snRNP. Mol Cell. 2010;38:439-51 pubmed publisher
    ..Tatcom1 consists of the core active P-TEFb, MLL-fusion partners involved in leukemia (AF9, AFF4, AFF1, ENL, and ELL), and PAF1 complex...
  8. Gu Y, Nakamura T, Alder H, Prasad R, Canaani O, Cimino G, et al. The t(4;11) chromosome translocation of human acute leukemias fuses the ALL-1 gene, related to Drosophila trithorax, to the AF-4 gene. Cell. 1992;71:701-8 pubmed
    ..This suggests that each 11q23 abnormality gives rise to a specific oncogenic fusion protein. ..
  9. Landlinger C, Oberleitner L, Gruber P, Noiges B, Yatsyk K, Santic R, et al. Active immunization against complement factor C5a: a new therapeutic approach for Alzheimer's disease. J Neuroinflammation. 2015;12:150 pubmed publisher
    ..Short antigenic peptides AFF1 and AFF2, which mimic a C-terminal epitope of C5a, were selected and formulated to vaccines...

More Information

Publications118 found, 100 shown here

  1. Akhter A, Mughal M, Elyamany G, Sinclair G, Azma R, Masir N, et al. Multiplexed automated digital quantification of fusion transcripts: comparative study with fluorescent in-situ hybridization (FISH) technique in acute leukemia patients. Diagn Pathol. 2016;11:89 pubmed publisher
    ..A high degree of correlation was noted for MLL/AF4 (85 sensitivity/100 specificity) and TCF3-PBX1 (75 % sensitivity/100 % specificity) probes...
  2. Chen X, Zhang J, Luo J, Wu S, Yuan G, Ma N, et al. CSTF2-Induced Shortening of the RAC1 3'UTR Promotes the Pathogenesis of Urothelial Carcinoma of the Bladder. Cancer Res. 2018;78:5848-5862 pubmed publisher
    ..GUAAU motif at proximal polyadenylation site of RAC1 attenuated the recruitment of two transcription factors AFF1 and AFF4, causing the defects in elongation...
  3. Afrin S, Zhang C, Meyer C, Stinson C, Pham T, Bruxner T, et al. Targeted Next-Generation Sequencing for Detecting MLL Gene Fusions in Leukemia. Mol Cancer Res. 2018;16:279-285 pubmed publisher
    ..Sensitivity assays demonstrated that AMP-E can detect MLL-AFF1 in MV4-11 cell dilutions of 10-7 and transcripts down to 0.005 copies/ng...
  4. Krystek P, Brandsma S, Leonards P, de Boer J. Exploring methods for compositional and particle size analysis of noble metal nanoparticles in Daphnia magna. Talanta. 2016;147:289-95 pubmed publisher measurement by asymmetric flow field flow fractionation coupled to inductively coupled plasma mass spectrometry (AF4-ICPMS) is challenging because the ENPs must stay stable regarding to particle size and composition...
  5. Bayart C, Peronin S, Jean E, Paladino J, Talaga P, Borgne M. The combined use of analytical tools for exploring tetanus toxin and tetanus toxoid structures. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1054:80-92 pubmed publisher
    ..SDS-PAGE, asymmetric flow field flow fractionation (AF4), fluorescence spectroscopy and circular dichroism (CD) were used to study formaldehyde modifications on the whole ..
  6. Ye H, Yang S, Zhang Y. MEG3 damages fetal endothelial function induced by gestational diabetes mellitus via AKT pathway. Eur Rev Med Pharmacol Sci. 2018;22:8553-8560 pubmed publisher
    ..luciferase reporter gene assay, microRNA-370-3p was found to be the target gene of MEG3 and directly targeted on AFF1. Moreover, MEG3 overexpression led to downregulated microRNA-370-3p and upregulated AFF1 mainly through inhibiting ..
  7. Li C, Bazzano L, Rao D, Hixson J, He J, Gu D, et al. Genome-wide linkage and positional association analyses identify associations of novel AFF3 and NTM genes with triglycerides: the GenSalt study. J Genet Genomics. 2015;42:107-17 pubmed publisher
    ..gene-based associations of charged multivesicular body protein 3 (CHMP3), ring finger protein 103 (RNF103), AF4/FMR2 family, member 3 (AFF3), and neurotrimin (NTM) with triglycerides (P = 4 × 10(-4), 1.00 × 10(-5), 2...
  8. Wright R, Slemmons K, Vaughan A. Estradiol induces gene proximity and MLL-MLLT3 fusion in an activation-induced cytidine deaminase-mediated pathway. Leuk Lymphoma. 2015;56:1460-5 pubmed publisher
    ..studies showed that 10 nM estradiol enhanced MLL transcription in addition to its common translocation partners, MLLT2 (AF4) and MLLT3 (AF9)...
  9. Velázquez Martínez J, González Cervantes R, Hernández Gallegos M, Mendiola R, Aparicio A, Ocampo M. Prebiotic potential of Agave angustifolia Haw fructans with different degrees of polymerization. Molecules. 2014;19:12660-75 pubmed publisher
    ..extracted from the agave stem by lixiviation and then purified by ultrafiltration and ion exchange chromatography: AFF1, AFF2 and AFF3 with high (3-60 fructose units), medium (2-40) and low (2-22) DP, respectively...
  10. Hrabovský Š, Folber F, Horacek J, Stehlikova O, Jelínková H, Salek C, et al. Comparison of Real-time Quantitative Polymerase Chain Reaction and Eight-color Flow Cytometry in Assessment of Minimal Residual Disease in Adult Acute Lymphoblastic Leukemia. Clin Lymphoma Myeloma Leuk. 2018;18:743-748 pubmed publisher
    ..using 8-color FCM and PCR of immunoglobulin or T-cell receptor gene clonal rearrangements and BCR-ABL1, KMT2A-AF4 and E2A-PBX1 fusion genes...
  11. Shi Y, Zhao Y, Zhang Y, AiErken N, Shao N, Ye R, et al. AFF3 upregulation mediates tamoxifen resistance in breast cancers. J Exp Clin Cancer Res. 2018;37:254 pubmed publisher
    ..Unfortunately, few molecular mediators of tamoxifen resistance have been described. Here, we describe AFF3 (AF4/FMR2 family member 3), which encodes a nuclear protein with transactivation potential that confers tamoxifen ..
  12. Liu W, Worms I, Herlin Boime N, Truffier Boutry D, Michaud Soret I, Mintz E, et al. Interaction of silver nanoparticles with metallothionein and ceruloplasmin: impact on metal substitution by Ag(i), corona formation and enzymatic activity. Nanoscale. 2017;9:6581-6594 pubmed publisher
    ..with on-line quantifications of their metal content were performed by asymmetrical flow field-flow fractionation (AF4) linked to inductively coupled plasma mass spectrometry (ICP-MS)...
  13. Schulze Gahmen U, Upton H, Birnberg A, Bao K, Chou S, Krogan N, et al. The AFF4 scaffold binds human P-TEFb adjacent to HIV Tat. elife. 2013;2:e00327 pubmed publisher
    ..HIV-1 Tat protein selectively recruits P-TEFb as part of a super elongation complex (SEC) organized on a flexible AFF1 or AFF4 scaffold...
  14. Astefanei A, Núñez O, Galceran M, Kok W, Schoenmakers P. Aggregation behavior of fullerenes in aqueous solutions: a capillary electrophoresis and asymmetric flow field-flow fractionation study. Anal Bioanal Chem. 2015;407:8035-45 pubmed publisher
    ..characterization of the suspected aggregates formed (size and shape), asymmetrical flow field-flow fractionation (AF4) and transmission electron microscopy (TEM) were used...
  15. Aguilera C, Gómez Llorente C, Tofe I, Gil Campos M, Cañete R, Gil Ã. Genome-wide expression in visceral adipose tissue from obese prepubertal children. Int J Mol Sci. 2015;16:7723-37 pubmed publisher
    ..NPRR3 and BHMT2), oxidative stress and extracellular matrix regulation (TNMD and NQO1), adipogenesis (CRYAB and AFF1) and inflammation (ANXA1); by contrast, only CALCRL gene expression was confirmed to be downregulated...
  16. Tu C, Du T, Shao C, Liu Z, Li L, Shen Y. Evaluating the potential of housekeeping genes, rRNAs, snRNAs, microRNAs and circRNAs as reference genes for the estimation of PMI. Forensic Sci Med Pathol. 2018;14:194-201 pubmed publisher
    ..Among the 11 candidate genes (β-actin, Gapdh, Rps18, 5S, 18S, U6, miR-133a, miR-122, circ-AFF1, LC-Ogdh and LC-LRP6), the following genes showed prioritized stability: miR-122, miR-133a and 18S in heart tissues;..
  17. Navarrete Meneses M, Salas Labadía C, Sanabrais Jiménez M, Santana Hernández J, Serrano Cuevas A, Juárez Velázquez R, et al. "Exposure to the insecticides permethrin and malathion induces leukemia and lymphoma-associated gene aberrations in vitro". Toxicol In Vitro. 2017;44:17-26 pubmed publisher
    ..The gene fusions IGH-BCL2, KMT2A-AFF1 and ETV6-RUNX1 were further analyzed with nested RT-PCR in PBMCs, and in K562 cells exposed to acute and chronic ..
  18. Bocca B, Caimi S, Senofonte O, Alimonti A, Petrucci F. ICP-MS based methods to characterize nanoparticles of TiO2 and ZnO in sunscreens with focus on regulatory and safety issues. Sci Total Environ. 2018;630:922-930 pubmed publisher
    ..The Asymmetric Flow-Field Flow Fractionation (AF4-FFF) as a pre-separation technique was on-line coupled to the Multi-Angle Light Scattering (MALS) and ICP-MS to ..
  19. Somasundaram B, Chang C, Fan Y, Lim P, Cardosa J, Lua L. Characterizing Enterovirus 71 and Coxsackievirus A16 virus-like particles production in insect cells. Methods. 2016;95:38-45 pubmed publisher
    ..High-resolution asymmetric flow field-flow fractionation couple with multi-angle light scattering (AF4-MALS) was used for the first time to characterize EV71 and CVA16 VLPs, displaying an average root mean square ..
  20. Niezabitowska E, Smith J, Prestly M, Akhtar R, von Aulock F, Lavallée Y, et al. Facile production of nanocomposites of carbon nanotubes and polycaprolactone with high aspect ratios with potential applications in drug delivery. RSC Adv. 2018;8:16444-16454 pubmed publisher
    ..scanning electron microscopy (SEM), atomic force microscopy (AFM) and asymmetric flow field flow fractionation (AF4)...
  21. Lemdani K, Salmon H, Gahoual R, Bessodes M, Scherman D, Houze P, et al. Assessment of the targeting specificity of a fluorescent albumin conceived as a preclinical agent of the liver function. Nanoscale. 2018;10:21151-21160 pubmed publisher
    ..was characterized by asymmetrical flow field-flow fractionation hyphenated to multi-angle light scattering (AF4-MALS)...
  22. Ibrahim F, Jamal R, Syafruddin S, Ab Mutalib N, Saidin S, MdZin R, et al. MicroRNA-200c and microRNA-31 regulate proliferation, colony formation, migration and invasion in serous ovarian cancer. J Ovarian Res. 2015;8:56 pubmed publisher
    ..Meanwhile, miR-31 gain-of-function led to the down-regulation of AF4/FMR2 family member 1 (AFF1) gene...
  23. Dawson M, Prinjha R, Dittmann A, Giotopoulos G, Bantscheff M, Chan W, et al. Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia. Nature. 2011;478:529-33 pubmed publisher
    ..therapeutic value, providing survival benefit in two distinct mouse models of murine MLL-AF9 and human MLL-AF4 leukaemia...
  24. Kim B, Lee H, Shin S, Lee S, Choi J. Clinical Evaluation of Massively Parallel RNA Sequencing for Detecting Recurrent Gene Fusions in Hematologic Malignancies. J Mol Diagn. 2019;21:163-170 pubmed publisher
    ..NGS panels detected 19 cases with recurrent translocations identified with RT-PCR, as well as a case with KMT2A-AFF1 with false-negative results on RT-PCR...
  25. Sogne V, Meier F, Klein T, Contado C. Investigation of zinc oxide particles in cosmetic products by means of centrifugal and asymmetrical flow field-flow fractionation. J Chromatogr A. 2017;1515:196-208 pubmed publisher
    ..the separation abilities of Centrifugal- and Asymmetrical Flow Field-Flow Fractionation techniques (CF3 and AF4, respectively), equipped with UV-vis, MALS and DLS detectors, in size sorting ZnO particles, both as pristine ..
  26. Ney Garcia D, de Souza M, de Figueiredo A, Othman M, Rittscher K, Abdelhay E, et al. Molecular characterization of KMT2A fusion partner genes in 13 cases of pediatric leukemia with complex or cryptic karyotypes. Hematol Oncol. 2016;: pubmed publisher five patients, all with acute lymphoblastic leukemia, MLLT1 in two patients, and MLLT10, MLLT4, MLLT11, and AFF1 in one patient each...
  27. Yokoyama A. Transcriptional activation by MLL fusion proteins in leukemogenesis. Exp Hematol. 2017;46:21-30 pubmed publisher
    ..Fusion proteins of MLL and a component of the AF4 family/ENL family/P-TEFb complex (AEP) are responsible for two-thirds of MLL-associated leukemia cases...
  28. Sun Y, Hu Y, Gao L, Xiao P, Lu J, Wu S, et al. The therapeutic efficacy of pediatric ALL patients with MLL gene rearrangement treated with CCLG-ALL2008 protocol. Eur Rev Med Pharmacol Sci. 2018;22:6020-6029 pubmed publisher
    ..05). Such factors as younger age than 2 years old, MLL/AF4 fusion gene, poor response to prednisone, or no complete remission (CR) on TP3 were poor prognostic parameters in ..
  29. Tan Y, Ye L, Xie F, Beyer A, Muench M, Wang J, et al. Respecifying human iPSC-derived blood cells into highly engraftable hematopoietic stem and progenitor cells with a single factor. Proc Natl Acad Sci U S A. 2018;115:2180-2185 pubmed publisher
    ..stem and progenitor cells (HSPCs) through transient expression of a single transcription factor, MLL-AF4 These induced HSPCs (iHSPCs) derived from iPSCs are able to fully reconstitute the human hematopoietic system ..
  30. Luo Z, Lin C, Woodfin A, Bartom E, Gao X, Smith E, et al. Regulation of the imprinted Dlk1-Dio3 locus by allele-specific enhancer activity. Genes Dev. 2016;30:92-101 pubmed publisher
    ..Previously, we identified the AF4/FMR2 (AFF) family protein AFF3 within the transcription elongation complex SEC-L3...
  31. Gomez Gonzalez M, Voegelin A, Garcia Guinea J, Bolea E, Laborda F, Garrido F. Colloidal mobilization of arsenic from mining-affected soils by surface runoff. Chemosphere. 2016;144:1123-31 pubmed publisher
    ..mode of As uptake using asymmetric flow field-flow fractionation coupled to inductively plasma mass spectrometry (AF4-ICP-MS) and X-ray absorption spectroscopy (XAS) at the As and Fe K-edges...
  32. Kang H, Wilson C, Harvey R, Chen I, Murphy M, Atlas S, et al. Gene expression profiles predictive of outcome and age in infant acute lymphoblastic leukemia: a Children's Oncology Group study. Blood. 2012;119:1872-81 pubmed publisher
    ..001). When infants with MLL-AFF1 were analyzed separately, a 7-gene classifier was developed that split them into 2 distinct groups with ..
  33. Vezočnik V, Rebolj K, Sitar S, Ota K, Tušek Žnidarič M, Štrus J, et al. Size fractionation and size characterization of nanoemulsions of lipid droplets and large unilamellar lipid vesicles by asymmetric-flow field-flow fractionation/multi-angle light scattering and dynamic light scattering. J Chromatogr A. 2015;1418:185-91 pubmed publisher
    Asymmetric-flow field-flow fractionation technique coupled to a multi-angle light-scattering detector (AF4-MALS) was used together with dynamic light-scattering (DLS) in batch mode and transmission electron microscopy (TEM) to study the ..
  34. Okuda H, Kanai A, Ito S, Matsui H, Yokoyama A. AF4 uses the SL1 components of RNAP1 machinery to initiate MLL fusion- and AEP-dependent transcription. Nat Commun. 2015;6:8869 pubmed publisher
    ..In most cases, MLL fuses with a gene encoding a component of the AEP (AF4 family/ENL family/P-TEFb) coactivator complex...
  35. Zhang H, Freitas D, Kim H, Fabijanic K, Li Z, Chen H, et al. Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation. Nat Cell Biol. 2018;20:332-343 pubmed publisher
    ..By employing asymmetric flow field-flow fractionation (AF4), we identified two exosome subpopulations (large exosome vesicles, Exo-L, 90-120 nm; small exosome vesicles, Exo-..
  36. Mudalige T, Qu H, Linder S. Asymmetric Flow-Field Flow Fractionation Hyphenated ICP-MS as an Alternative to Cloud Point Extraction for Quantification of Silver Nanoparticles and Silver Speciation: Application for Nanoparticles with a Protein Corona. Anal Chem. 2015;87:7395-401 pubmed publisher
    ..Herein, we developed an analytical platform consisting of asymmetric flow-field flow fractionation (AF4) coupled with inductively coupled plasma mass spectroscopy (ICP-MS) for the speciation and quantification of silver ..
  37. Zielke C, Fuentes C, Piculell L, Nilsson L. Co-elution phenomena in polymer mixtures studied by asymmetric flow field-flow fractionation. J Chromatogr A. 2018;1532:251-256 pubmed publisher
    ..Only few analytical techniques are suitable for the task. AF4-MALS-dRI is highly suited for the task, but the analysis can nevertheless be especially challenging for ..
  38. Furtado L, Bundschuh M, Metcalfe C. Monitoring the Fate and Transformation of Silver Nanoparticles in Natural Waters. Bull Environ Contam Toxicol. 2016;97:449-55 pubmed publisher
    ..coupled plasma mass spectrometry (spICP-MS) and asymmetric flow field flow fractionation with on-line ICP-MS (AF4-ICP-MS) are analytical techniques that show potential for quantitative analysis of AgNPs in aquatic matrices at ..
  39. Eskelin K, Lampi M, Meier F, Moldenhauer E, Bamford D, Oksanen H. Asymmetric flow field flow fractionation methods for virus purification. J Chromatogr A. 2016;1469:108-119 pubmed publisher
    ..Asymmetric flow field flow fractionation (AF4) is an attractive alternative method for virus purification because it is a rapid and gentle separation method that ..
  40. Zielke C, Kosík O, Ainalem M, Lovegrove A, Stradner A, Nilsson L. Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter. PLoS ONE. 2017;12:e0172034 pubmed publisher terms of molecular size and molar mass distributions using asymmetric flow field-flow fractionation (AF4) coupled to multiangle light scattering (MALS), differential refractive index (dRI) and fluorescence (FL) detection...
  41. Wang W, Chen M, Guo L, Wang W. Size partitioning and mixing behavior of trace metals and dissolved organic matter in a South China estuary. Sci Total Environ. 2017;603-604:434-444 pubmed publisher
    ..distribution of organic matter and selected metals were quantified using asymmetric flow field-flow fractionation (AF4) and ICP-MS...
  42. Nakamura T, Alder H, Gu Y, Prasad R, Canaani O, Kamada N, et al. Genes on chromosomes 4, 9, and 19 involved in 11q23 abnormalities in acute leukemia share sequence homology and/or common motifs. Proc Natl Acad Sci U S A. 1993;90:4631-5 pubmed
    ..Stretches abundant in basic amino acids are also present in the three proteins. These results suggest that the different proteins fused to ALL-1 polypeptide(s) provide similar functional domains. ..
  43. Bueno C, Montes R, Catalina P, Rodriguez R, Menendez P. Insights into the cellular origin and etiology of the infant pro-B acute lymphoblastic leukemia with MLL-AF4 rearrangement. Leukemia. 2011;25:400-10 pubmed publisher
    ..Over 90% of infant ALLs are pro-B ALL harboring the leukemic fusion MLL-AF4. Despite the fact that major achievements have provided a better understanding about the etiology of infant MLL-AF4+..
  44. Mak A, Nixon A, Moffat J. The mixed lineage leukemia (MLL) fusion-associated gene AF4 promotes CD133 transcription. Cancer Res. 2012;72:1929-34 pubmed publisher
    ..The transcription factor AF4 was identified following a comparative analysis between the two screens...
  45. Limsuwanachot N, Siriboonpiputtana T, Karntisawiwat K, Chareonsirisuthigul T, Chuncharunee S, Rerkamnuaychoke B. Multiplex RT-PCR Assay for Detection of Common Fusion Transcripts in Acute Lymphoblastic Leukemia and Chronic Myeloid Leukemia Cases. Asian Pac J Cancer Prev. 2016;17:677-84 pubmed
    ..a multiplex RT-PCR assay for detection of the 4 most frequent translocations in ALL (BCR-ABL, TEL-AML1, MLL-AF4, and E2A- PBX1)...
  46. Bertrand F, Spengeman J, Shah N, LeBien T. B-cell development in the presence of the MLL/AF4 oncoprotein proceeds in the absence of HOX A7 and HOX A9 expression. Leukemia. 2003;17:2454-9 pubmed
    ..leukemia (ALL) is frequently characterized by the t(4;11)(q21;q23) cytogenetic abnormality encoding the MLL/AF4 oncogene, increased HOX gene expression and a pro-B/monocytoid phenotype...
  47. Tomizawa D, Kato M, Takahashi H, Fujimura J, Inukai T, Fukushima T, et al. Favorable outcome in non-infant children with MLL-AF4-positive acute lymphoblastic leukemia: a report from the Tokyo Children's Cancer Study Group. Int J Hematol. 2015;102:602-10 pubmed publisher
    ..0 % [standard error (SE), 9.7 %] and 64.0 % (SE 9.6 %), respectively. Notably, 9/12 cases with MLL-AF4-positive ALL are alive in continuous CR with a 75.0 % (SE 12.5 %) EFS rate...
  48. Choi J, Lee S, Linares Pastén J, Nilsson L. Study on oligomerization of glutamate decarboxylase from Lactobacillus brevis using asymmetrical flow field-flow fractionation (AF4) with light scattering techniques. Anal Bioanal Chem. 2018;410:451-458 pubmed publisher
    In this work, asymmetrical flow field-flow fractionation (AF4) coupled with UV/Vis, multi-angle light scattering (MALS), and differential refractive index (dRI) detectors (AF4-UV-MALS-dRI) was employed for analysis of glutamate ..
  49. Chopra A, Soni S, Verma D, Kumar D, Dwivedi R, Vishwanathan A, et al. Prevalence of common fusion transcripts in acute lymphoblastic leukemia: A report of 304 cases. Asia Pac J Clin Oncol. 2015;11:293-8 pubmed publisher
    ..This study was conducted to determine the frequency of common fusion transcripts BCR-ABL, TEL-AML1, MLL-AF4 and E2A-PBX1 for B-ALL and SIL-TAL1 for T-ALL as seen at a tertiary care center in India...
  50. Khurshid S, Keaney J, Ellinor P, Lubitz S. A Simple and Portable Algorithm for Identifying Atrial Fibrillation in the Electronic Medical Record. Am J Cardiol. 2016;117:221-5 pubmed publisher
    ..In total, we performed manual adjudication of AF in 700 subjects. Three AF schemas (AF1, AF2, and AF4) achieved positive predictive value (PPV) >0.9. Two control schemas achieved PPV >0...
  51. Hinna A, Hupfeld S, Kuntsche J, Brandl M. The use of asymmetrical flow field-flow fractionation with on-line detection in the study of drug retention within liposomal nanocarriers and drug transfer kinetics. J Pharm Biomed Anal. 2016;124:157-163 pubmed publisher
    ..The donor- and acceptor-liposomes were subsequently separated using asymmetrical flow field-flow fractionation (AF4), during which the sample is exposed to a large volume of eluent which corresponds to a dilution factor of ..
  52. Fuentes C, Zielke C, Prakash M, Kumar P, Peñarrieta J, Eliasson A, et al. The effect of baking and enzymatic treatment on the structural properties of wheat starch. Food Chem. 2016;213:768-774 pubmed publisher
    ..bread and its molecular properties were characterized using (1)H NMR and asymmetric flow field-flow fractionation (AF4) connected to multi-angle light scattering (MALS) and other detectors...
  53. Sánchez García L, Bolea E, Laborda F, Cubel C, Ferrer P, Gianolio D, et al. Size determination and quantification of engineered cerium oxide nanoparticles by flow field-flow fractionation coupled to inductively coupled plasma mass spectrometry. J Chromatogr A. 2016;1438:205-15 pubmed publisher
    ..Two modalities of FFFF (Asymmetric Flow- and Hollow Fiber-Flow Field Flow Fractionation, AF4 and HF5, respectively) are compared, and their advantages and limitations discussed...
  54. Chang Y, Shih Y, Su C, Ho H. Comparison of three analytical methods to measure the size of silver nanoparticles in real environmental water and wastewater samples. J Hazard Mater. 2017;322:95-104 pubmed publisher
    ..techniques are compared, including hydrodynamic chromatography (HDC), asymmetric flow field flow fractionation (AF4) and single particle inductively coupled plasma-mass spectrometry (SP-ICP-MS)...
  55. Ai X, Zhang Y, Shi X, Zheng Y, Zhang L, Wang X, et al. [Application of Combined Detection of Fusion Gene and BIOMED-2 Standardized Ig Gene Rearrangement System in Childhood B-cell Acute Lymphoblastic Leukemia]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2016;24:352-7 pubmed publisher
    ..Out of 251 children with B-ALL, 77 cases were TEL-AML1(+) , 28 cases were E2A-PBX1(+) , 10 cases were MLL-AF4(+) , 11 cases were BCR-ABL(+) , the total positive rate was 50.2%, 82.5% showed IgH VH-JH rearrangement, 53...
  56. Meleshko A, Belevtsev M, Savitskaja T, Potapnev M. The incidence of T-cell receptor gene rearrangements in childhood B-lineage acute lymphoblastic leukemia is related to immunophenotype and fusion oncogene expression. Leuk Res. 2006;30:795-800 pubmed
    ..Low frequency of clonal TCRG rearrangements was associated with expression of E2A/PBX chimeric oncogene. We suggest that TCRG and TCRD clonal rearrangements in leukemic B-cells are associated with early stages of their differentiation. ..
  57. Astefanei A, Kok W, Bäuerlein P, Núñez O, Galceran M, de Voogt P, et al. Characterization of aggregates of surface modified fullerenes by asymmetrical flow field-flow fractionation with multi-angle light scattering detection. J Chromatogr A. 2015;1408:197-206 pubmed publisher
    ..In this work, asymmetrical flow-field flow fractionation (AF4) coupled to multi-angle light scattering (MALS) was used for the first time to study the size distribution of ..
  58. Kotaki R, Kotani A. A single miRNA complements transcription factor deficiency through the TGF pathway at time of B-cell lineage determination. Rinsho Ketsueki. 2017;58:792-797 pubmed publisher
    ..miR-126 induced B-cell differentiation in MLL-AF4 acute lymphoblastic leukemia (ALL) without upregulation of TCF3/E2A, EBF1, and PAX5...
  59. Furudate K, Okimoto Y, Ando K, Taneyama Y, Ochiai H, Kakuda H. [Congenital leukemia showing lineage switch following induction chemotherapy and attaining long-term remission after HLA-haploidentical stem cell transplantation]. Rinsho Ketsueki. 2017;58:2243-2249 pubmed publisher
    ..We describe a case of a newborn female with initial diagnosis of MLL-AF4 positive B-precursor acute lymphoblastic leukemia, who developed lineage switch to acute monocytic leukemia ..
  60. Frestedt J, Hilden J, Kersey J. AF4/FEL, a gene involved in infant leukemia: sequence variations, gene structure, and possible homology with a genomic sequence on 5q31. DNA Cell Biol. 1996;15:669-78 pubmed
    ..This unique genetic rearrangement fuses the MLL/ALL-1/HRX-Htrx gene at 11q23 with the AF4/FEL gene at 4q21...
  61. Chen W, Li Q, Hudson W, Kumar A, Kirchhof N, Kersey J. A murine Mll-AF4 knock-in model results in lymphoid and myeloid deregulation and hematologic malignancy. Blood. 2006;108:669-77 pubmed
    The 2 most frequent human MLL hematopoietic malignancies involve either AF4 or AF9 as fusion partners; each has distinct biology but the role of the fusion partner is not clear...
  62. Cen H, Leng R, Wang W, Zhou M, Feng C, Chen G, et al. Association of AFF1 rs340630 and AFF3 rs10865035 polymorphisms with systemic lupus erythematosus in a Chinese population. Immunogenetics. 2012;64:935-8 pubmed publisher
    The aim of this study was to evaluate whether two single-nucleotide polymorphisms (SNPs), AF4/FMR2 family, member 1 (AFF1) rs340630 and AF4/FMR2 family, member 3 (AFF3) rs10865035, show significant evidence for association with systemic ..
  63. Mbonye U, Karn J. Transcriptional control of HIV latency: cellular signaling pathways, epigenetics, happenstance and the hope for a cure. Virology. 2014;454-455:328-39 pubmed publisher
    ..An in-depth comprehensive understanding of the molecular control of HIV-1 transcription should inform the development of optimal combinatorial reactivation strategies that are intended to purge the latent viral reservoir...
  64. Malouf C, Ottersbach K. Molecular processes involved in B cell acute lymphoblastic leukaemia. Cell Mol Life Sci. 2018;75:417-446 pubmed publisher and summarise the biological, clinical and epidemiological knowledge on B cell acute lymphoblastic leukaemia of four well characterised subtypes: t(4;11) MLL-AF4, t(12;21) ETV6-RUNX1, t(1;19) E2A-PBX1 and t(9;22) BCR-ABL1.
  65. Tamai H, Inokuchi K. Establishment of MLL/AF4 transgenic mice with the phenotype of lymphoblastic leukemia or lymphoma. J Nippon Med Sch. 2013;80:326-7 pubmed
  66. Okada Y, Shimane K, Kochi Y, Tahira T, Suzuki A, Higasa K, et al. A genome-wide association study identified AFF1 as a susceptibility locus for systemic lupus eyrthematosus in Japanese. PLoS Genet. 2012;8:e1002455 pubmed publisher
    ..9%). In addition, we identified a novel association of a variant in the AF4/FMR2 family, member 1 (AFF1) gene at 4q21 with SLE susceptibility (rs340630; P?=?8.3×10(-9), odds ratio?=?1.21)...
  67. Felix C, Hosler M, Slater D, Megonigal M, Lovett B, Williams T, et al. Duplicated regions of AF-4 intron 4 at t(4;11) translocation breakpoints. Mol Diagn. 1999;4:269-83 pubmed
    ..Reciprocal fusion transcripts were expressed. Intronic sequence of AF-4 is useful for molecular diagnosis of t(4;11). Duplicated intronic regions suggest staggered chromosomal breakage. ..
  68. Muñoz López A, Romero Moya D, Prieto C, Ramos Mejia V, Agraz Doblas A, Varela I, et al. Development Refractoriness of MLL-Rearranged Human B Cell Acute Leukemias to Reprogramming into Pluripotency. Stem Cell Reports. 2016;7:602-618 pubmed publisher
    ..Conversely, MLL-AF4-overexpressing hematopoietic stem cells/B progenitors were successfully reprogrammed, indicating that B cell origin ..
  69. Rifai Chai -, Naik G, Sai Ho Ling -, Tran Y, Craig A, Nguyen H. Channels selection using independent component analysis and scalp map projection for EEG-based driver fatigue classification. Conf Proc IEEE Eng Med Biol Soc. 2017;2017:1808-1811 pubmed publisher
    ..fatigue assessment as chosen channels, which included AF3, F3, FC1, FC5, T7, CP5, P3, O1, P4, P8, CP6, T8, FC2, F8, AF4, FP2. The result of fatigue vs. alert classification of the selected 16 channels yielded a sensitivity of 76...
  70. Biswas D, Milne T, Basrur V, Kim J, Elenitoba Johnson K, Allis C, et al. Function of leukemogenic mixed lineage leukemia 1 (MLL) fusion proteins through distinct partner protein complexes. Proc Natl Acad Sci U S A. 2011;108:15751-6 pubmed publisher
    ..Here we first identify natural complexes (purified through fusion partners AF9, AF4, and ELL) with overlapping components, different elongation activities, and different cofactor associations that ..
  71. Prieto C, Marschalek R, Kühn A, Bursen A, Bueno C, Menendez P. The AF4-MLL fusion transiently augments multilineage hematopoietic engraftment but is not sufficient to initiate leukemia in cord blood CD34+ cells. Oncotarget. 2017;8:81936-81941 pubmed publisher
    ..Unlike other MLL translocations, MLL-AF4-induced proB-ALL is exceptionally difficult to model in mice/humans...
  72. Nakamura T, Canaani E, Croce C. Oncogenic All1 fusion proteins target Drosha-mediated microRNA processing. Proc Natl Acad Sci U S A. 2007;104:10980-5 pubmed
    ..Further, we demonstrate coimmunoprecipitation of the All1/Af4 and All1/Af9 fusions with Drosha, disrupted by treatment with DNase I...
  73. Pui C, Campana D. Age-related differences in leukemia biology and prognosis: the paradigm of MLL-AF4-positive acute lymphoblastic leukemia. Leukemia. 2007;21:593-4 pubmed
  74. Nilson K, Price D. The Role of RNA Polymerase II Elongation Control in HIV-1 Gene Expression, Replication, and Latency. Genet Res Int. 2011;2011:726901 pubmed publisher
    ..HIV, the causative agent of AIDS, is a worldwide health concern. It is hoped that knowledge of the mechanisms regulating the expression of the HIV genome will lead to treatments and ultimately a cure. ..
  75. Domer P, Fakharzadeh S, Chen C, Jockel J, Johansen L, Silverman G, et al. Acute mixed-lineage leukemia t(4;11)(q21;q23) generates an MLL-AF4 fusion product. Proc Natl Acad Sci U S A. 1993;90:7884-8 pubmed
    ..encodes a fusion RNA and predicted chimeric protein between the 11q23 gene MLL and a 4q21 gene designated AF4. The sequence of the complete open reading frame for this fusion transcript reveals the MLL protein to have ..
  76. Morrissey J, Tkachuk D, Milatovich A, Francke U, Link M, Cleary M. A serine/proline-rich protein is fused to HRX in t(4;11) acute leukemias. Blood. 1993;81:1124-31 pubmed
    ..These features are similar to predicted t(11;19) fusion proteins, suggesting that HRX consistently contributes a novel DNA-binding motif to at least two different chimeric proteins in acute leukemias. ..
  77. Gué M, Sun J, Boudier T. Simultaneous localization of MLL, AF4 and ENL genes in interphase nuclei by 3D-FISH: MLL translocation revisited. BMC Cancer. 2006;6:20 pubmed
    ..than 40 partners, the study of the relative positions of the MLL gene with both the most frequent partner gene (AF4) and a less frequent partner gene (ENL), should elucidate the MLL translocation mechanism...
  78. Castaño J, Herrero A, Bursen A, Gonzalez F, Marschalek R, Gutierrez N, et al. Expression of MLL-AF4 or AF4-MLL fusions does not impact the efficiency of DNA damage repair. Oncotarget. 2016;7:30440-52 pubmed publisher
    The most frequent rearrangement of the human MLL gene fuses MLL to AF4 resulting in high-risk infant B-cell acute lymphoblastic leukemia (B-ALL). MLL fusions are also hallmark oncogenic events in secondary acute myeloid leukemia...
  79. Safenkova I, Slutskaya E, Panferov V, Zherdev A, Dzantiev B. Complex analysis of concentrated antibody-gold nanoparticle conjugates' mixtures using asymmetric flow field-flow fractionation. J Chromatogr A. 2016;1477:56-63 pubmed publisher
    ..Herein, we have applied asymmetrical flow field-flow fractionation (AF4) to identify the conjugates' state...
  80. Zhou C, Xiong Q, Zhu X, Du W, Deng P, Li X, et al. AFF1 and AFF4 differentially regulate the osteogenic differentiation of human MSCs. Bone Res. 2017;5:17044 pubmed publisher
    b>AFF1 and AFF4 belong to the AFF (AF4/FMR2) family of proteins, which function as scaffolding proteins linking two different transcription elongation factors, positive elongation factor b (P-TEFb) and ELL1/2, in super elongation complexes (..
  81. Liu H, Westergard T, Cashen A, Piwnica Worms D, Kunkle L, Vij R, et al. Proteasome inhibitors evoke latent tumor suppression programs in pro-B MLL leukemias through MLL-AF4. Cancer Cell. 2014;25:530-42 pubmed publisher
    ..Furthermore, proteasome inhibition conferred preliminary benefit to patients with MLL-AF4 leukemia...
  82. Kerry J, Godfrey L, Repapi E, Tapia M, Blackledge N, Ma H, et al. MLL-AF4 Spreading Identifies Binding Sites that Are Distinct from Super-Enhancers and that Govern Sensitivity to DOT1L Inhibition in Leukemia. Cell Rep. 2017;18:482-495 pubmed publisher
    ..Translocations of the mixed-lineage leukemia (MLL) gene produce fusion proteins such as MLL-AF4 that disrupt epigenetic pathways and cause poor-prognosis leukemias...
  83. Raffini L, Slater D, Rappaport E, Lo Nigro L, Cheung N, Biegel J, et al. Panhandle and reverse-panhandle PCR enable cloning of der(11) and der(other) genomic breakpoint junctions of MLL translocations and identify complex translocation of MLL, AF-4, and CDK6. Proc Natl Acad Sci U S A. 2002;99:4568-73 pubmed
    ..of the predicted Cdk6-MLL fusion protein in the genesis of the ALL, which also contains an in-frame MLL-AF4 transcript. The sequences in these three cases show additional MLL genomic breakpoint heterogeneity...
  84. Bolinsson H, Lu Y, Hall S, Nilsson L, HÃ¥kansson A. An alternative method for calibration of flow field flow fractionation channels for hydrodynamic radius determination: The nanoemulsion method (featuring multi angle light scattering). J Chromatogr A. 2018;1533:155-163 pubmed publisher
    ..suggests a novel method for determination of the channel height in asymmetrical flow field-flow fractionation (AF4), which can be used for calibration of the channel for hydrodynamic radius determinations...
  85. Bizarro S, Cerveira N, Correia C, Lisboa S, Peixoto A, Norton L, et al. Molecular characterization of a rare MLL-AF4 (MLL-AFF1) fusion rearrangement in infant leukemia. Cancer Genet Cytogenet. 2007;178:61-4 pubmed
    The t(4;11)(q21;q23) involving the genes MLL and AF4 (alias for AFF1) is detected in 50-70% of infant leukemia...
  86. Liang Q, Wu D, Qiu B, Han N. [Present situation and development trends of asymmetrical flow field-flow fractionation]. Se Pu. 2017;35:918-926 pubmed publisher
    ..Asymmetrical flow field-flow fractionation (AF4) is a vital subvariant of FFF, which applying a vertical flow field as the second dimension force field...
  87. Ajuba I, Madu A, Okocha C, Ibegbulam O, Okpala I, Nna O. Frequency and clinical impact of ETV6/RUNX1, AF4-MLL, and BCR/ABL fusion genes on features of acute lymphoblastic leukemia at presentation. Niger J Clin Pract. 2016;19:237-41 pubmed publisher
    ..Three major translocations (ETV6-RUNX1, BCR-ABL, and AF4-MLL) in acute lymphoblastic leukemia (ALL) have been shown to affect treatment outcome...
  88. Sitar S, Vezočnik V, Macek P, Kogej K, Pahovnik D, Zagar E. Pitfalls in Size Characterization of Soft Particles by Dynamic Light Scattering Online Coupled to Asymmetrical Flow Field-Flow Fractionation. Anal Chem. 2017;89:11744-11752 pubmed publisher
    An asymmetrical flow field-flow fractionation (AF4) technique coupled to a multiangle light scattering (MALS) detector with an embedded dynamic light scattering (DLS) module was introduced to study the size characteristics and shape of ..
  89. Ui A, Nagaura Y, Yasui A. Transcriptional elongation factor ENL phosphorylated by ATM recruits polycomb and switches off transcription for DSB repair. Mol Cell. 2015;58:468-82 pubmed publisher
    ..Our data suggest that ATM-dependent phosphorylation of ENL functions as switch from elongation to Polycomb-mediated repression to preserve genome integrity. ..
  90. Leach B, Kuntimaddi A, Schmidt C, Cierpicki T, Johnson S, Bushweller J. Leukemia fusion target AF9 is an intrinsically disordered transcriptional regulator that recruits multiple partners via coupled folding and binding. Structure. 2013;21:176-183 pubmed publisher
    ..We determined the structure of the AF9 AHD in complex with the elongation factor AF4 and show that aliphatic residues, which are conserved in each of the AF9 binding partners, form an integral part of ..
  91. Benedikt A, Baltruschat S, Scholz B, Bursen A, Arrey T, Meyer B, et al. The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures. Leukemia. 2011;25:135-44 pubmed publisher
    Expression of the AF4-MLL fusion protein in murine hematopoietic progenitor/stem cells results in the development of proB acute lymphoblastic leukemia...
  92. Eskelin K, Lampi M, Meier F, Moldenhauer E, Bamford D, Oksanen H. Halophilic viruses with varying biochemical and biophysical properties are amenable to purification with asymmetrical flow field-flow fractionation. Extremophiles. 2017;21:1119-1132 pubmed publisher
    ..Asymmetrical flow field-flow fractionation (AF4) is a gentle native chromatography-like technique for size-based separation...