ACAT1

Summary

Gene Symbol: ACAT1
Description: acetyl-CoA acetyltransferase 1
Alias: ACAT, MAT, THIL, acetyl-CoA acetyltransferase, mitochondrial, acetoacetyl Coenzyme A thiolase, acetoacetyl-CoA thiolase, acetyl-Coenzyme A acetyltransferase 1, mitochondrial acetoacetyl-CoA thiolase, testicular tissue protein Li 198
Species: human
Products:     ACAT1

Top Publications

  1. Kang J, Cheng B, Jiang L. [PPAR? signal transduction pathway in the foam cell formation induced by visfatin]. Sheng Li Xue Bao. 2010;62:427-32 pubmed
    ..in the expression of ATP binding cassette transporter A1 (ABCA1) and acyl-CoA:cholesterol acyltransferase 1 (ACAT1) induced by visfatin and to discuss the mechanism of foam cell formation induced by visfatin...
  2. Ohta T, Takata K, Katsuren K, Fukuyama S. The influence of the acyl-CoA:cholesterol acyltransferase-1 gene (-77G-->A) polymorphisms on plasma lipid and apolipoprotein levels in normolipidemic and hyperlipidemic subjects. Biochim Biophys Acta. 2004;1682:56-62 pubmed
    Acyl-CoA:cholesterol acyltransferase (ACAT) plays important roles in cellular cholesterol homeostasis. Two isoforms of ACAT have been reported (ACAT-1 and ACAT-2)...
  3. Yoshinaka Y, Shibata H, Kobayashi H, Kuriyama H, Shibuya K, Tanabe S, et al. A selective ACAT-1 inhibitor, K-604, stimulates collagen production in cultured smooth muscle cells and alters plaque phenotype in apolipoprotein E-knockout mice. Atherosclerosis. 2010;213:85-91 pubmed publisher
    Acyl-coenzyme A:cholesterol O-acyltransferase-1 (ACAT-1) plays an essential role in macrophage foam cell formation and progression of atherosclerosis...
  4. Li Q, Bai H, Fan P. [Analysis of acyl-coenzyme A: cholesterol acyltransferase 1 polymorphism in patients with endogenous hypertriglyceridemia in Chinese population]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2008;25:206-10 pubmed
    To investigate the polymorphism of acyl-coenzyme A: cholesterol acyltransferase 1(ACAT1) gene and its relationship with endogenous hypertriglyceridemia (HTG) in Chinese population...
  5. Chen J, Zhao X, Yang L, Hu G, Lu M, Xiong Y, et al. RNA secondary structures located in the interchromosomal region of human ACAT1 chimeric mRNA are required to produce the 56-kDa isoform. Cell Res. 2008;18:921-36 pubmed publisher
    We have previously reported that the human ACAT1 gene produces a chimeric mRNA through the interchromosomal processing of two discontinuous RNAs transcribed from chromosomes 1 and 7...
  6. Wu D, Yin R, Aung L, Li Q, Yan T, Zeng X, et al. Sex-specific association of ACAT-1 rs1044925 SNP and serum lipid levels in the hypercholesterolemic subjects. Lipids Health Dis. 2012;11:9 pubmed publisher
    Acyl-CoA:cholesterol acyltransferase (ACAT) is a key enzyme in cellular cholesterol homeostasis and in atherosclerosis...
  7. Osborne C, West E, Bate C. The phospholipase A2 pathway controls a synaptic cholesterol ester cycle and synapse damage. J Cell Sci. 2018;131: pubmed publisher
    ..PAF facilitated the incorporation of acyl-coenzyme A:cholesterol acyltransferase (ACAT)-1 into Aβ-PrPC-cPLA2-COX-2-Fyn complexes...
  8. Davis W, Boyd J, Ile K, Tew K. Human ATP-binding cassette transporter-2 (ABCA2) positively regulates low-density lipoprotein receptor expression and negatively regulates cholesterol esterification in Chinese hamster ovary cells. Biochim Biophys Acta. 2004;1683:89-100 pubmed
    ..containing LPDS, however, no significant difference was measured in acylcoenzyme A:cholesterol acyltransferase (ACAT) activity in cell-free extracts of CHO and CHOA2 cells...
  9. Hwang K, Hwang Y, Song J. Cholesterol-lowering effect of Aralia elata (Miq.) Seem via the activation of SREBP-2 and the LDL receptor. J Chin Med Assoc. 2017;80:630-635 pubmed publisher
    ..However, the AE extract did not affect the gene expression of acetyl-coenzyme A acetyltransferase (ACAT) in either the HepG2 cells or mice...

More Information

Publications114 found, 100 shown here

  1. Davis W. The ATP-binding cassette transporter-2 (ABCA2) regulates esterification of plasma membrane cholesterol by modulation of sphingolipid metabolism. Biochim Biophys Acta. 2014;1841:168-79 pubmed publisher
    ..an altered bilayer distribution of the sphingolipid ceramide that inhibited acylCoA:cholesterol acyltransferase (ACAT) activity and cholesterol esterification...
  2. Bowdridge E, Goravanahally M, Inskeep E, Flores J. Activation of Adenosine Monophosphate-Activated Protein Kinase Is an Additional Mechanism That Participates in Mediating Inhibitory Actions of Prostaglandin F2Alpha in Mature, but Not Developing, Bovine Corpora Lutea. Biol Reprod. 2015;93:7 pubmed publisher
    ..Protein expression of LDLR decreased at 2 and 4 h, while those of ACAT1 and STAR increased 4 h after PGF2alpha...
  3. Mulye M, Zapata B, Gilk S. Altering lipid droplet homeostasis affects Coxiella burnetii intracellular growth. PLoS ONE. 2018;13:e0192215 pubmed publisher
    ..LD formation with the pharmacological inhibitors triacsin C or T863, or knocking out acyl-CoA transferase-1 (acat-1) in alveolar macrophages, increased C. burnetii growth at least 2-fold...
  4. De Gonzalo Calvo D, López Vilaró L, Nasarre L, Pérez Olabarría M, Vázquez T, Escuin D, et al. Intratumor cholesteryl ester accumulation is associated with human breast cancer proliferation and aggressive potential: a molecular and clinicopathological study. BMC Cancer. 2015;15:460 pubmed publisher
    ..An increased expression of acetyl-Coenzyme A acetyltransferase 1 (ACAT1) in CE-rich tumors was also reported...
  5. Cheng B, Wan J, Wang Y, Mei C, Liu W, Ke L, et al. Ghrelin inhibits foam cell formation via simultaneously down-regulating the expression of acyl-coenzyme A:cholesterol acyltransferase 1 and up-regulating adenosine triphosphate-binding cassette transporter A1. Cardiovasc Pathol. 2010;19:e159-66 pubmed publisher
    ..We assessed the expression of acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT-1) and adenosine triphosphate (ATP)-binding cassette transporter A1 (ABCA1), which have been implicated in ..
  6. Xu L, Wang Y, Li P, Feng B. Advanced glycation end products increase lipids accumulation in macrophages through upregulation of receptor of advanced glycation end products: increasing uptake, esterification and decreasing efflux of cholesterol. Lipids Health Dis. 2016;15:161 pubmed publisher
    ..regulating the main molecular expression including CD36, Scavenger receptors (SR) A2, HMG-CoA reductase (HMGCR), ACAT1 and ATP-binding cassette transporter G1 (ABCG1)...
  7. Law C, Lam C, Ching C, Yau K, Ho T, Lai C, et al. NMR-based urinalysis for beta-ketothiolase deficiency. Clin Chim Acta. 2015;438:222-5 pubmed publisher
    ..Diagnosis of beta-ketothiolase deficiency was further established by molecular genetic studies of ACAT1 gene of the proband...
  8. Chowdhury S, Lecomte V, Erlich J, Maloney C, Morris M. Paternal High Fat Diet in Rats Leads to Renal Accumulation of Lipid and Tubular Changes in Adult Offspring. Nutrients. 2016;8: pubmed publisher
    ..b>Acat1, involved in entry of fatty acid for beta-oxidation, was significantly upregulated, possibly to counteract ..
  9. Tsintzas K, Stephens F, Snijders T, Wall B, Cooper S, Mallinson J, et al. Intramyocellular lipid content and lipogenic gene expression responses following a single bout of resistance type exercise differ between young and older men. Exp Gerontol. 2017;93:36-45 pubmed publisher
    ..indicating increased inflammatory response (IL6, IkBalpha, CREB1) and impaired fat metabolism and TCA cycle (LPL, ACAT1, SUCLG1) in older compared with younger individuals...
  10. Xu G, Watanabe T, Iso Y, Koba S, Sakai T, Nagashima M, et al. Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis. Circ Res. 2009;105:500-10 pubmed publisher
    ..Macrophage foam cell formation, characterized by cholesterol ester accumulation, is modulated by scavenger receptor class A (SR-A), acyl-coenzyme A:cholesterol acyltransferase (ACAT)1, and ATP-binding cassette transporter (ABC)A1.
  11. Yamauchi Y, Yokoyama S, Chang T. ABCA1-dependent sterol release: sterol molecule specificity and potential membrane domain for HDL biogenesis. J Lipid Res. 2016;57:77-88 pubmed publisher
    ..Blocking ACAT activity increases the cholesterol contents of this domain...
  12. Ding L, Biswas S, Morton R, Smith J, Hay N, Byzova T, et al. Akt3 deficiency in macrophages promotes foam cell formation and atherosclerosis in mice. Cell Metab. 2012;15:861-72 pubmed publisher
    ..Mechanistically, Akt3 suppresses foam cell formation by reducing lipoprotein uptake and promoting ACAT-1 degradation via the ubiquitin-proteasome pathway...
  13. Davidson M. Therapies targeting exogenous cholesterol uptake: new insights and controversies. Curr Atheroscler Rep. 2011;13:95-100 pubmed publisher
    ..transcription factors such as LXR that affect sterol transporters NPC1L1, ABCG5/G8, and ABCG1, and enzymes such as ACAT-2...
  14. Ribeiro A, de Lacerda R, Correia D, Brunialti Godard A, de Miranda D, Campos V, et al. Possible involvement of ACSS2 gene in alcoholism. J Neural Transm (Vienna). 2017;124:1151-1158 pubmed publisher
    ..Seven genes were picked out (Acss2, Acss3, Acat1, Acsl1, Acaa2, Hadh, and Hadhb) and the mRNA level of the Acss2 gene was increased only in the "compulsive-like" ..
  15. Zeng Y, Zhang L, Hu Z, Yang Q, Ma M, Liu B, et al. Association of protein Z and factor VII gene polymorphisms with risk of cerebral hemorrhage: a case-control and a family-based association study in a Chinese Han population. J Genet. 2016;95:383-8 pubmed
    ..of PZ rs2273971, and four haplotypes carrying or crossing the 'A' allele of PZ rs2273971: haplotypes CA, ACAA, ACAT and ACAAT of PZ and FVII genes, may confer protection against CH (Z= -1.882, P = 0.049; Z= -2.000, P = 0.045; Z= -2...
  16. da Rosa R, Piccoli B, da Silva F, Dornelles L, Rocha J, Sonego M, et al. Synthesis, antioxidant and antitumoral activities of 5'-arylchalcogeno-3-aminothymidine (ACAT) derivatives. Medchemcomm. 2017;8:408-414 pubmed publisher
    ..The chalcogeno-aminothymidines presented prominent activity in the tests for both biological properties, showing a direct relation with the chalcogenium atom. ..
  17. Zhan Y, Zhang X, Xiong Y, Li B, Nan F. Design and synthesis of simple, yet potent and selective non-ring-A pyripyropene A-based inhibitors of acyl-coenzyme A: cholesterol acyltransferase 2 (ACAT2). Org Biomol Chem. 2016;14:747-751 pubmed publisher
    ..Representative compound (-)-3 exhibited potent activity against ACAT2 and greater selectivity for ACAT2 than for ACAT1.
  18. Cho W, Kim J, Paeng J, Suh M, Kim Y, Kang H, et al. Can Combined Bypass Surgery at Middle Cerebral Artery Territory Save Anterior Cerebral Artery Territory in Adult Moyamoya Disease?. Neurosurgery. 2017;80:431-438 pubmed publisher
    Patients with moyamoya disease are frequently encountered with improved symptoms related to anterior cerebral artery territory (ACAt) and middle cerebral artery territory (MCAt) after bypass surgery at MCAt.
  19. Garcia Bermudez J, Birsoy K. Drugging ACAT1 for Cancer Therapy. Mol Cell. 2016;64:856-857 pubmed publisher
    ..can promote glycolysis by phosphorylating and stabilizing the tetrameric form of mitochondrial acetyl-coA acetyltransferase 1 (ACAT1). The authors further identify a small molecule ACAT1 inhibitor that displays anti-cancer effects.
  20. Rogers M, Liu J, Song B, Li B, Chang C, Chang T. Acyl-CoA:cholesterol acyltransferases (ACATs/SOATs): Enzymes with multiple sterols as substrates and as activators. J Steroid Biochem Mol Biol. 2015;151:102-7 pubmed publisher
    ..In mammals, there are two ACAT genes that encode two different enzymes, ACAT1 and ACAT2. Both are allosteric enzymes that can be activated by a variety of sterols...
  21. Saxton D, Brown P, Seguinot Medina S, Eckstein L, Carpenter D, Miller P, et al. Environmental health and justice and the right to research: institutional review board denials of community-based chemical biomonitoring of breast milk. Environ Health. 2015;14:90 pubmed publisher
    ..Alaska Community Action on Toxics (ACAT) responded to community requests for breast milk biomonitoring and conceived the Breast Milk Pilot Study (BMPS)...
  22. Kano M, Fukao T, Yamaguchi S, Orii T, Osumi T, Hashimoto T. Structure and expression of the human mitochondrial acetoacetyl-CoA thiolase-encoding gene. Gene. 1991;109:285-90 pubmed
    ..deficiency at the gene level, we analyzed the structure of the human mitochondrial acetoacetyl-CoA thiolase (MAT; EC 2.3.1.9)-encoding gene (MAT)...
  23. Rózsa M, Ford A, Flicker L. Assessing people with dementia: the role of the aged care assessment team and memory clinics. Intern Med J. 2016;46:1081-8 pubmed publisher
    A cross-sectional survey of all patients reviewed by the aged care assessment team (ACAT) service and memory clinics between 1 January and 31 December 2012. The ACAT catchment included an estimated 14?325 people over the age of 70...
  24. Chen X, Liang H, Song Q, Xu X, Cao D. Insulin promotes progression of colon cancer by upregulation of ACAT1. Lipids Health Dis. 2018;17:122 pubmed publisher
    ..Immunohistochemistry was used to detect the expression of acyl coenzyme A: cholesterol acyltransferase1 (ACAT1), and we analyzed the correlation between hyperglycemia and ACAT1, hyperglycemia and metastasis...
  25. Yamauchi Y, Chang C, Hayashi M, Abe Dohmae S, Reid P, Chang T, et al. Intracellular cholesterol mobilization involved in the ABCA1/apolipoprotein-mediated assembly of high density lipoprotein in fibroblasts. J Lipid Res. 2004;45:1943-51 pubmed
    ..ApoA-I induced a rapid decrease of the cellular cholesterol compartment that is in equilibrium with the ACAT-accessible pool in cells that generate cholesterol-rich HDL...
  26. Ngo Sock E, Mayer G, Lavoie J. Combined Effects of Rosuvastatin and Exercise on Gene Expression of Key Molecules Involved in Cholesterol Metabolism in Ovariectomized Rats. PLoS ONE. 2016;11:e0159550 pubmed publisher
    ..training (Tr) on expression of genes involved in cholesterol metabolism (LDLR, PCSK9, LRP-1, SREBP-2, IDOL, ACAT-2 and HMGCR) in the liver of eight week-old ovariectomized (Ovx) rats...
  27. Hamdi Y, Soucy P, Kuchenbaeker K, Pastinen T, Droit A, Lemaçon A, et al. Association of breast cancer risk in BRCA1 and BRCA2 mutation carriers with genetic variants showing differential allelic expression: identification of a modifier of breast cancer risk at locus 11q22.3. Breast Cancer Res Treat. 2017;161:117-134 pubmed publisher
    ..This association was absent in BRCA2 carriers (p = 0.57). The 11q22.3 region notably encompasses genes such as ACAT1, NPAT, and ATM...
  28. Watanabe H, Yamaguchi S, Kimura M, Wakazono A, Song X, Fukao T, et al. Practical assay method of cytosolic acetoacetyl-CoA thiolase by rapid release of cytosolic enzymes from cultured lymphocytes using digitonin. Tohoku J Exp Med. 1998;184:29-38 pubmed
    ..Hence, this method will prove to be useful for accurate assessment of defects of CT as well as T2 or SCOT, all involved in ketone body catabolism. ..
  29. Repa J, Buhman K, Farese R, Dietschy J, Turley S. ACAT2 deficiency limits cholesterol absorption in the cholesterol-fed mouse: impact on hepatic cholesterol homeostasis. Hepatology. 2004;40:1088-97 pubmed
    Acyl CoA:cholesterol acyltransferase (ACAT) 2 is the major cholesterol-esterifying enzyme in mouse enterocytes and hepatocytes. Male ACAT2(+/+) and ACAT2(-/ -) mice were fed chow containing added cholesterol (0%-0.500% w/w) for 24 days...
  30. Ge J, Zhai W, Cheng B, He P, Qi B, Lu H, et al. Insulin induces human acyl-coenzyme A: cholesterol acyltransferase1 gene expression via MAP kinases and CCAAT/enhancer-binding protein ?. J Cell Biochem. 2013;114:2188-98 pubmed publisher
    ..investigate the relationship between hyperinsulinemia and atherosclerosis, we investigated whether insulin induced ACAT1 gene expression and found that insulin up-regulated ACAT1 mRNA, protein and enzyme activity in human THP-1 cells ..
  31. Drouin Chartier J, Tremblay A, Lemelin V, Lépine M, Lamarche B, Couture P. Ezetimibe increases intestinal expression of the LDL receptor gene in dyslipidaemic men with insulin resistance. Diabetes Obes Metab. 2016;18:1226-1235 pubmed publisher
    ..0%; P?=?.04) and acetyl-Coenzyme A acetyltransferase 2 (ACAT-2) mRNA expression (+12.5%; P?=?.03)...
  32. Grünert S, Schmitt R, Schlatter S, Gemperle Britschgi C, Balci M, Berg V, et al. Clinical presentation and outcome in a series of 32 patients with 2-methylacetoacetyl-coenzyme A thiolase (MAT) deficiency. Mol Genet Metab. 2017;122:67-75 pubmed publisher
    ..It is caused by mutations in the ACAT1 gene and may present with metabolic ketoacidosis...
  33. Zhu Y, Chen C, Li J, Cheng J, Jang M, Kim K. In vitro exploration of ACAT contributions to lipid droplet formation during adipogenesis. J Lipid Res. 2018;59:820-829 pubmed publisher
    ..Pharmacological inhibition of ACAT activity suppressed intracellular cholesterol and CE levels, and reduced expression of genes involved in ..
  34. Schutgens R, Middleton B, vd Blij J, Oorthuys J, Veder H, Vulsma T, et al. Beta-ketothiolase deficiency in a family confirmed by in vitro enzymatic assays in fibroblasts. Eur J Pediatr. 1982;139:39-42 pubmed
  35. Liu F, Wang Y, Xu J, Liu F, Hu R, Deng H. Effects of Porphyromonas gingivalis lipopolysaccharide on the expression of key genes involved in cholesterol metabolism in macrophages. Arch Med Sci. 2016;12:959-967 pubmed
    ..lectin-like oxidized LDL receptor-1 (LOX-1), ATP-binding cassette G1 (ABCG1), and acetyl CoA acyltransferase 1 (ACAT1) expression levels were measured by western blot and qRT-PCR...
  36. Fukao T, Nguyen H, Nguyen N, Vu D, Can N, Pham A, et al. A common mutation, R208X, identified in Vietnamese patients with mitochondrial acetoacetyl-CoA thiolase (T2) deficiency. Mol Genet Metab. 2010;100:37-41 pubmed publisher
    ..DNA diagnosis of T2 deficiency may be applicable to the Vietnamese population. ..
  37. Kim J, Lee H, Jeong S, Lee M, Kim S. Essential oil of Pinus koraiensis leaves exerts antihyperlipidemic effects via up-regulation of low-density lipoprotein receptor and inhibition of acyl-coenzyme A: cholesterol acyltransferase. Phytother Res. 2012;26:1314-9 pubmed publisher
    ..11%), d-limonene (21.01%), ?-pinene (16.74%) and borneol (11.52%). Overall, the findings suggest that EOPK can be a potent pharmaceutical agent for the prevention and treatment of hyperlipidemia. ..
  38. Sundvold H, Sundvold Gjerstad V, Malerød Fjeld H, Haglund K, Stenmark H, Malerød L. Arv1 promotes cell division by recruiting IQGAP1 and myosin to the cleavage furrow. Cell Cycle. 2016;15:628-43 pubmed publisher
    ..Here we report a novel role of the putative lipid transporter ACAT-related protein required for viability 1 (Arv1) during telophase...
  39. Abdelkreem E, Akella R, Dave U, Sane S, Otsuka H, Sasai H, et al. Clinical and Mutational Characterizations of Ten Indian Patients with Beta-Ketothiolase Deficiency. JIMD Rep. 2017;35:59-65 pubmed publisher
    ..thiolase (T2) deficiency) is an inherited disease of isoleucine catabolism and ketone body utilization caused by ACAT1 mutations. We identified ten Indian patients who manifested with ketoacidotic episodes of variable severity...
  40. Huang L, Melton E, Li H, Sohn P, Rogers M, Mulligan Kehoe M, et al. Myeloid Acyl-CoA:Cholesterol Acyltransferase 1 Deficiency Reduces Lesion Macrophage Content and Suppresses Atherosclerosis Progression. J Biol Chem. 2016;291:6232-44 pubmed publisher
    Acyl-CoA:cholesterol acyltransferase 1 (Acat1) converts cellular cholesterol to cholesteryl esters and is considered a drug target for treating atherosclerosis...
  41. Mahapatra D, Bharti S. Therapeutic potential of chalcones as cardiovascular agents. Life Sci. 2016;148:154-72 pubmed publisher
    ..ester transfer protein (CETP), diacylglycerol acyltransferase (DGAT), acyl-coenzyme A: cholesterol acyltransferase (ACAT), pancreatic lipase (PL), lipoprotein lipase (LPL), calcium (Ca(2+))/potassium (K(+)) channel, COX-1, TXA2 and TXB2...
  42. Fukao T, Nakamura H, Song X, Nakamura K, Orii K, Kohno Y, et al. Characterization of N93S, I312T, and A333P missense mutations in two Japanese families with mitochondrial acetoacetyl-CoA thiolase deficiency. Hum Mutat. 1998;12:245-54 pubmed
    ..By contrast, Asn93 is located near the active site and may function to maintain a local loop structure. The mutation of Asn93 could directly disrupt disposition of the active site. ..
  43. Antalis C, Arnold T, Rasool T, Lee B, Buhman K, Siddiqui R. High ACAT1 expression in estrogen receptor negative basal-like breast cancer cells is associated with LDL-induced proliferation. Breast Cancer Res Treat. 2010;122:661-70 pubmed publisher
    ..The differences corresponded to greater mRNA and protein levels of acyl-CoA:cholesterol acyltransferase 1 (ACAT1), higher ACAT activity, higher caveolin-1 protein levels, greater LDL uptake, and lower de novo cholesterol ..
  44. Khund Sayeed S, He X, Holzberg T, Wang J, Rajagopal D, Upadhyay S, et al. 5-Hydroxymethylcytosine in E-box motifs ACAT|GTG and ACAC|GTG increases DNA-binding of the B-HLH transcription factor TCF4. Integr Biol (Camb). 2016;8:936-45 pubmed publisher
    ..In contrast, 5hmC dramatically enhanced TCF4 binding to E-box motifs ACAT|GTG and ACAC|GTG, being better bound than any 8-mer containing cytosine...
  45. Huang L, Nishi K, Li S, Ho T, Dong R, Chang C, et al. Acyl-coenzyme A:cholesterol acyltransferase 1 - significance of single-nucleotide polymorphism at residue 526 and the role of Pro347 near the fifth transmembrane domain. FEBS J. 2014;281:1773-83 pubmed publisher
    ..Mammals have two isoenzymes: ACAT1 and ACAT2. Both enzymes are drug targets for treating human diseases. ACAT1 is present in various cell types...
  46. Miller Pinsler L, Wells P. Embryonic catalase protects against ethanol embryopathies in acatalasemic mice and transgenic human catalase-expressing mice in embryo culture. Toxicol Appl Pharmacol. 2015;287:232-9 pubmed publisher
    ..Cg-Cat(b)/J catalase-deficient, acatalasemic (aCat) mouse embryos or their wild-type C3HeB/FeJ (C3H WT) controls, were explanted on gestational day (GD) 9 (plug=GD 1),..
  47. Ouyang H, Zhou H, He C, Zhang G, Chen Y, Wang T, et al. [The oxLDL/?2GPI/anti-?2GPI antibody complex promotes A7r5 chemotaxis, migration and lipid accumulation]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2017;33:1622-1627 pubmed
    ..monocyte chemoattractant protein-1 (MCP-1), matrix metalloproteinase-9 (MMP-9) and acetyl-CoA acetyltransferase 1 (ACAT1) were evaluated by real-time quantitative PCR...
  48. Fukao T, Yamaguchi S, Tomatsu S, Orii T, Frauendienst Egger G, Schrod L, et al. Evidence for a structural mutation (347Ala to Thr) in a German family with 3-ketothiolase deficiency. Biochem Biophys Res Commun. 1991;179:124-9 pubmed
    ..This report is apparently the first definition of a mutant allele for 3KTD, at the gene level. ..
  49. Fu Y, Luo L, Luo N, Garvey W. Lipid metabolism mediated by adipocyte lipid binding protein (ALBP/aP2) gene expression in human THP-1 macrophages. Atherosclerosis. 2006;188:102-11 pubmed
    ..Moreover, we determined that the expression of acyl-coenzyme A: cholesterol-acyltransferase 1 (ACAT1) gene was up-regulated by the overexpression of ALBP gene, and on the other hand, the ATP-binding cassette A1 (..
  50. Shibuya Y, Chang C, Huang L, Bryleva E, Chang T. Inhibiting ACAT1/SOAT1 in microglia stimulates autophagy-mediated lysosomal proteolysis and increases Aβ1-42 clearance. J Neurosci. 2014;34:14484-501 pubmed publisher
    Acyl-CoA:cholesterol acyltransferase 1 (ACAT1) is a resident endoplasmic reticulum enzyme that prevents the buildup of cholesterol in membranes by converting it to cholesterol esters...
  51. Lagor W, Tong F, Jarrett K, Lin W, Conlon D, Smith M, et al. Deletion of murine Arv1 results in a lean phenotype with increased energy expenditure. Nutr Diabetes. 2015;5:e181 pubmed publisher
    b>ACAT-related enzyme 2 required for viability 1 (ARV1) is a putative lipid transporter of the endoplasmic reticulum that is conserved across eukaryotic species...
  52. Kern R, Zarek C, Lindholm Perry A, Kuehn L, Snelling W, Freetly H, et al. Ruminal expression of the NQO1, RGS5, and ACAT1 genes may be indicators of feed efficiency in beef steers. Anim Genet. 2017;48:90-92 pubmed publisher
    ..0009) and regulator of G-protein signaling 5 (RGS5; P = 0.01). A third gene, acetyl-CoA acetyltransferase 1 (ACAT1; P = 0.06), displayed a trend toward association with RFI...
  53. Fukao T, Yamaguchi S, Wakazono A, Orii T, Hoganson G, Hashimoto T. Identification of a novel exonic mutation at -13 from 5' splice site causing exon skipping in a girl with mitochondrial acetoacetyl-coenzyme A thiolase deficiency. J Clin Invest. 1994;93:1035-41 pubmed
    ..The role of exon sequences in the splicing mechanism is indicated by the exon skipping which occurred with an exonic mutation. ..
  54. Fukao T, Yamaguchi S, Kano M, Orii T, Fujiki Y, Osumi T, et al. Molecular cloning and sequence of the complementary DNA encoding human mitochondrial acetoacetyl-coenzyme A thiolase and study of the variant enzymes in cultured fibroblasts from patients with 3-ketothiolase deficiency. J Clin Invest. 1990;86:2086-92 pubmed
    ..These results suggest that different mechanisms are involved in the enzyme defects in the four patients. ..
  55. Fukao T, Yamaguchi S, Scriver C, Dunbar G, Wakazono A, Kano M, et al. Molecular studies of mitochondrial acetoacetyl-coenzyme A thiolase deficiency in the two original families. Hum Mutat. 1993;2:214-20 pubmed
    ..The JB allele associates with Dutch ancestry (no consanguinity) and the JM allele with Chilean ancestry (distant consanguinity). ..
  56. Kanome T, Watanabe T, Nishio K, Takahashi K, Hongo S, Miyazaki A. Angiotensin II upregulates acyl-CoA:cholesterol acyltransferase-1 via the angiotensin II Type 1 receptor in human monocyte-macrophages. Hypertens Res. 2008;31:1801-10 pubmed publisher
    ..Acyl-CoA:cholesterol acyltransferase-1 (ACAT1) converts intracellular free cholesterol into cholesterol ester (CE) for storage in lipid droplets, and promotes ..
  57. Sakashita N, Chang C, Lei X, Fujiwara Y, Takeya M, Chang T. Cholesterol loading in macrophages stimulates formation of ER-derived vesicles with elevated ACAT1 activity. J Lipid Res. 2010;51:1263-72 pubmed publisher
    b>ACAT1 is normally a resident enzyme in the endoplasmic reticulum (ER). We previously showed that treating macrophages with denatured LDL causes a large increase in ER-derived, ACAT1-positive vesicles...
  58. Liang Y, Yang X, Ma L, Cai X, Wang L, Yang C, et al. Homocysteine-mediated cholesterol efflux via ABCA1 and ACAT1 DNA methylation in THP-1 monocyte-derived foam cells. Acta Biochim Biophys Sin (Shanghai). 2013;45:220-8 pubmed publisher
    ..Acyl-coenzyme A:cholesterol acyltransferase-1 (ACAT1) promotes accumulation of cholesterol ester in macrophages, thereby resulting in the foam cell formation, a ..
  59. Yamamoto K, Tajima Y, Hasegawa A, Takahashi Y, Kojima M, Watanabe R, et al. Contrasting effects of stanniocalcin-related polypeptides on macrophage foam cell formation and vascular smooth muscle cell migration. Peptides. 2016;82:120-127 pubmed publisher
    ..Expression of acyl-CoA:cholesterol acyltransferase-1 (ACAT1) was significantly suppressed by STC1(18-247) but stimulated by STC2(80-100) and STC2(85-99)...
  60. Fukao T, Yamaguchi S, Orii T, Hashimoto T. Molecular basis of beta-ketothiolase deficiency: mutations and polymorphisms in the human mitochondrial acetoacetyl-coenzyme A thiolase gene. Hum Mutat. 1995;5:113-20 pubmed
    ..A correlation between clinical phenotype and genotype in T2 deficiency seems unlikely. ..
  61. Sewell A, Herwig J, Wiegratz I, Lehnert W, Niederhoff H, Song X, et al. Mitochondrial acetoacetyl-CoA thiolase (beta-ketothiolase) deficiency and pregnancy. J Inherit Metab Dis. 1998;21:441-2 pubmed
  62. Kellner Weibel G, Luke S, Rothblat G. Cytotoxic cellular cholesterol is selectively removed by apoA-I via ABCA1. Atherosclerosis. 2003;171:235-43 pubmed
    ..Treatment with an acyl coenzyme-A:cholesterol acyltransferase (ACAT) inhibitor, in the absence of extracellular acceptors, produced hydrolysis of stored esterified cholesterol (EC) ..
  63. Rajapakse D, Peterson K, Mishra S, Wistow G. Serum starvation of ARPE-19 changes the cellular distribution of cholesterol and Fibulin3 in patterns reminiscent of age-related macular degeneration. Exp Cell Res. 2017;361:333-341 pubmed publisher
    ..AMD) associated EFEMP1/Fibulin3 (Fib3); and levels of acyl-coenzyme A (CoA): cholesterol acyltransferases (ACAT) ACAT1, ACAT2 and Apolipoprotein B (ApoB) in ARPE-19 cells cultured in serum-supplemented and serum-free media...
  64. Abbaszadeh H, Peyvandi A, Sadeghi Y, Safaei A, Zamanian Azodi M, Khoramgah M, et al. Er:YAG Laser and Cyclosporin A Effect on Cell Cycle Regulation of Human Gingival Fibroblast Cells. J Lasers Med Sci. 2017;8:143-149 pubmed publisher
    ..YWHAZ, VCP, HNRNPU, YWHAE, UBA52, CLTC, FUS and IGHG1 as key proteins while similar analysis revealed that ACAT1, CTSD, ALDOA, ANXA2, PRDX1, LGALS3, ARHGDI and EEF1A1 are the crucial proteins related to the effect of drug...
  65. Raman J, Fritz T, Gerken T, Jamison O, Live D, Liu M, et al. The catalytic and lectin domains of UDP-GalNAc:polypeptide alpha-N-Acetylgalactosaminyltransferase function in concert to direct glycosylation site selection. J Biol Chem. 2008;283:22942-51 pubmed publisher
    ..Local sequence recognition by the catalytic domain differs between hT2 and hT10 in that hT10 requires a pre-existing GalNAc residue while hT2 does not. ..
  66. Hu L, Li J, Cai H, Yao W, Xiao J, Li Y, et al. Avasimibe: A novel hepatitis C virus inhibitor that targets the assembly of infectious viral particles. Antiviral Res. 2017;148:5-14 pubmed publisher
    ..Our results showed that avasimibe, an inhibitor of acyl coenzyme A:cholesterol acyltransferase (ACAT), exhibited marked pan-genotypic inhibitory activity and superior inhibition against HCV when combined with DAAs...
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    ..When the PCR-amplified genomic fragments around exon 11 were sequenced, an AG to AC mutation at the 3' splice site of intron 10 was detected. This mutation is presumed to be responsible for exon 11 skipping. ..
  68. Fukao T, Matsuo N, Zhang G, Urasawa R, Kubo T, Kohno Y, et al. Single base substitutions at the initiator codon in the mitochondrial acetoacetyl-CoA thiolase (ACAT1/T2) gene result in production of varying amounts of wild-type T2 polypeptide. Hum Mutat. 2003;21:587-92 pubmed
    ..in a Japanese patient (Patient GK30) with mitochondrial acetoacetyl-CoA thiolase (T2) gene deficiency (ACAT1 deficiency); c.149delC was on the other allele...
  69. Guo Z, Chang C, Chang T. Functionality of the seventh and eighth transmembrane domains of acyl-coenzyme A:cholesterol acyltransferase 1. Biochemistry. 2007;46:10063-71 pubmed
    Acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT1) is a resident enzyme in the endoplasmic reticulum. ACAT1 is a homotetrameric protein and contains nine transmembrane domains (TMDs)...
  70. Maurer G, Brucker D, Bähr O, Harter P, Hattingen E, Walenta S, et al. Differential utilization of ketone bodies by neurons and glioma cell lines: a rationale for ketogenic diet as experimental glioma therapy. BMC Cancer. 2011;11:315 pubmed publisher
    ..dehydrogenase 1 and 2 (BDH1 and 2), 3-oxoacid-CoA transferase 1 (OXCT1) and acetyl-CoA acetyltransferase 1 (ACAT1) were expressed at the mRNA and protein level in all glioma cell lines...
  71. Lo Y, Lin S, Chang S, Chan C, Lyu K, Chang J, et al. Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer. J Cell Mol Med. 2015;19:744-59 pubmed publisher
    ..Mitochondrial proteins such as acetyl-CoA acetyltransferase (ACAT1) and malate dehydrogenase (MDH2) have not been reported with the roles on the formation of doxorubicin resistance ..
  72. Saadane A, Mast N, Dao T, Ahmad B, Pikuleva I. Retinal Hypercholesterolemia Triggers Cholesterol Accumulation and Esterification in Photoreceptor Cells. J Biol Chem. 2016;291:20427-39 pubmed publisher
    ..OS were also found to express the cholesterol-esterifying enzyme acyl-coenzyme A:cholesterol acyltransferase (ACAT1), but not lecithin-cholesterol acyltransferase (LCAT), and to differ from humans in retinal expression of ACAT1...
  73. Wojcik M, Wierenga K, Rodan L, Sahai I, Ferdinandusse S, Genetti C, et al. Beta-Ketothiolase Deficiency Presenting with Metabolic Stroke After a Normal Newborn Screen in Two Individuals. JIMD Rep. 2018;39:45-54 pubmed publisher
    ..It results from biallelic pathogenic variants in the ACAT1 gene, encoding mitochondrial beta-ketothiolase...
  74. Wakazono A, Fukao T, Yamaguchi S, Hori T, Orii T, Lambert M, et al. Molecular, biochemical, and clinical characterization of mitochondrial acetoacetyl-coenzyme A thiolase deficiency in two further patients. Hum Mutat. 1995;5:34-42 pubmed
    ..Cotransfection of two cDNAs containing N158D and T297M suggested that heterotetramer formation reduces residual activity in GK11 cells. ..
  75. An S, Jang Y, Park J, Kwon B, Paik Y, Jeong T. Inhibition of acyl-coenzyme A:cholesterol acyltransferase stimulates cholesterol efflux from macrophages and stimulates farnesoid X receptor in hepatocytes. Exp Mol Med. 2008;40:407-17 pubmed
    ..the mechanism of spontaneous cholesterol efflux induced by acyl-coenzyme A:cholesterol acyltransferase (ACAT) inhibition, and how an alteration of cholesterol metabolism in macrophages impacts on that in HepG2 cells...
  76. Bzoma B, Debska Slizien A, Dudziak M, Raczynska K, Slizień W, Brylowska A, et al. [Genetic predisposition to systemic complications of arterial hypertension in maintenance haemodialysis patients]. Pol Merkur Lekarski. 2008;25:209-16 pubmed
    ..Direct ophtalmoscopy seems to be an insufficient method in the estimation of the systemic complications of hypertension in haemodialysed patients. ..
  77. Nakazawa T, Miyanoki Y, Urano Y, Uehara M, Saito Y, Noguchi N. Effect of vitamin E on 24(S)-hydroxycholesterol-induced necroptosis-like cell death and apoptosis. J Steroid Biochem Mol Biol. 2017;169:69-76 pubmed publisher
    ..Esterification of 24S-OHC followed by lipid droplet (LD) formation due to acyl-CoA:cholesterol acyltransferase 1 (ACAT1) are key events in 24S-OHC-induced cell death in atRA-treated SH-SY5Y cells as demonstrated by inhibition of cell ..
  78. Su Y, Dove D, Major A, Hasty A, Boone B, Linton M, et al. Reduced ABCA1-mediated cholesterol efflux and accelerated atherosclerosis in apolipoprotein E-deficient mice lacking macrophage-derived ACAT1. Circulation. 2005;111:2373-81 pubmed
    Macrophage acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT1) and apolipoprotein E (apoE) have been implicated in regulating cellular cholesterol homeostasis and therefore play critical roles in foam cell formation...
  79. Sakurai S, Fukao T, Haapalainen A, Zhang G, Yamada K, Lilliu F, et al. Kinetic and expression analyses of seven novel mutations in mitochondrial acetoacetyl-CoA thiolase (T2): identification of a Km mutant and an analysis of the mutational sites in the structure. Mol Genet Metab. 2007;90:370-8 pubmed
    ..The probable effect of the other mutations on the T2 tertiary structure was also evaluated. ..
  80. Thümmler S, Dupont D, Acquaviva C, Fukao T, De Ricaud D. Different clinical presentation in siblings with mitochondrial acetoacetyl-CoA thiolase deficiency and identification of two novel mutations. Tohoku J Exp Med. 2010;220:27-31 pubmed
    ..In conclusion, the siblings with the same T2 gene mutations present different clinical severity. Diagnostic testing for asymptomatic siblings is important in the management of T2-deficient families. ..
  81. Wu D, Yin R, Cao X, Chen W. Association between single nucleotide polymorphism rs1044925 and the risk of coronary artery disease and ischemic stroke. Int J Mol Sci. 2014;15:3546-59 pubmed publisher
    The present study was performed to clarify the association between the acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) single nucleotide polymorphism (SNP) rs1044925 and the risk of coronary artery disease (CAD) and ischemic stroke (IS) ..
  82. Ohmoto T, Nishitsuji K, Yoshitani N, Mizuguchi M, Yanagisawa Y, Saito H, et al. K604, a specific acyl‑CoA:cholesterol acyltransferase 1 inhibitor, suppresses proliferation of U251‑MG glioblastoma cells. Mol Med Rep. 2015;12:6037-42 pubmed publisher
    ..levels of cholesteryl ester and simultaneous expression of acyl‑CoA:cholesterol acyltransferase 1 (ACAT1) in tumor cells indicated that cholesterol esterification is critical to tumor growth...
  83. Yang B, Smith D. In Silico Absorption Analysis of Valacyclovir in Wildtype and Pept1 Knockout Mice Following Oral Dose Escalation. Pharm Res. 2017;34:2349-2361 pubmed publisher
    ..Simulations were conducted using a mechanistic advanced compartmental absorption and transit (ACAT) model implemented in GastroPlus™...
  84. Hai Q, Ritchey B, Robinet P, Alzayed A, Brubaker G, Zhang J, et al. Quantitative Trait Locus Mapping of Macrophage Cholesterol Metabolism and CRISPR/Cas9 Editing Implicate an ACAT1 Truncation as a Causal Modifier Variant. Arterioscler Thromb Vasc Biol. 2018;38:83-91 pubmed publisher
    ..the Soat1 (sterol O-acyltransferase 1) gene, encoding Acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT1), which esterifies free cholesterol...
  85. Xu T, Cardoso F, Pineda A, Trevisi E, Shen X, Rosa F, et al. Grain challenge affects systemic and hepatic molecular biomarkers of inflammation, stress, and metabolic responses to a greater extent in Holstein than Jersey cows. J Dairy Sci. 2017;100:9153-9162 pubmed publisher
    ..Genes related to ketogenesis (HMGCS2 and ACAT1) were upregulated in Jerseys, and gluconeogenic genes (PDK4 and PCK1) were upregulated in Holstein cows fed HIG, ..
  86. Yin R, Wu D, Wu J, Cao X, Aung L, Miao L, et al. Interactions of several lipid-related gene polymorphisms and cigarette smoking on blood pressure levels. Int J Biol Sci. 2012;8:685-96 pubmed publisher
    ..Genotyping of ATP-binding cassette transporter A1 (ABCA-1) V825I, acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) rs1044925, low density lipoprotein receptor (LDL-R) Ava?, hepatic lipase gene (LIPC) -250G>A, endothelial ..
  87. Fukao T, Song X, Mitchell G, Yamaguchi S, Sukegawa K, Orii T, et al. Enzymes of ketone body utilization in human tissues: protein and messenger RNA levels of succinyl-coenzyme A (CoA):3-ketoacid CoA transferase and mitochondrial and cytosolic acetoacetyl-CoA thiolases. Pediatr Res. 1997;42:498-502 pubmed
    ..The relative amounts of T2 and SCOT were similar in all tissues except for liver (T2 > > SCOT) and brain (SCOT > T2). The observed distribution of SCOT, T2, and CT is consistent with current views of their physiologic roles. ..
  88. Saraon P, Cretu D, Musrap N, Karagiannis G, Batruch I, Drabovich A, et al. Quantitative proteomics reveals that enzymes of the ketogenic pathway are associated with prostate cancer progression. Mol Cell Proteomics. 2013;12:1589-601 pubmed publisher
    ..After analyzing the remaining enzymes of this pathway (ACAT1, BDH1, HMGCL, and OXCT1), we observed increased expression of these proteins in the LNCaP-SF cells, which was ..
  89. Fan J, Shan C, Kang H, Elf S, Xie J, Tucker M, et al. Tyr phosphorylation of PDP1 toggles recruitment between ACAT1 and SIRT3 to regulate the pyruvate dehydrogenase complex. Mol Cell. 2014;53:534-48 pubmed publisher
    ..Moreover, we identified mitochondrial ACAT1 and SIRT3 as the upstream acetyltransferase and deacetylase, respectively, of PDHA1 and PDP1, while knockdown of ..
  90. Gobeau N, Stringer R, De Buck S, Tuntland T, Faller B. Evaluation of the GastroPlusâ„¢ Advanced Compartmental and Transit (ACAT) Model in Early Discovery. Pharm Res. 2016;33:2126-39 pubmed publisher
    ..exposure predictions obtained early in drug discovery with a generic GastroPlus Advanced Compartmental And Transit (ACAT) model based on the in vivo intravenous blood concentration-time profile, in silico properties (lipophilicity, pKa) ..
  91. Fan J, Lin R, Xia S, Chen D, Elf S, Liu S, et al. Tetrameric Acetyl-CoA Acetyltransferase 1 Is Important for Tumor Growth. Mol Cell. 2016;64:859-874 pubmed publisher
    Mitochondrial acetyl-CoA acetyltransferase 1 (ACAT1) regulates pyruvate dehydrogenase complex (PDC) by acetylating pyruvate dehydrogenase (PDH) and PDH phosphatase...