bacteriorhodopsin

Summary

Gene Symbol: bacteriorhodopsin
Description: bacterio-opsin
Species: Halobacterium sp. NRC-1

Top Publications

  1. ncbi Structure of bacteriorhodopsin at 1.55 A resolution
    H Luecke
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, 92697, USA
    J Mol Biol 291:899-911. 1999
  2. ncbi Deformation of helix C in the low temperature L-intermediate of bacteriorhodopsin
    Karl Edman
    Department of Chemistry and Bioscience, Chalmers University of Technology, Box 462, S 40530 Gothenburg, Sweden
    J Biol Chem 279:2147-58. 2004
  3. pmc Crystal structure of the bromide-bound D85S mutant of bacteriorhodopsin: principles of ion pumping
    Marc T Facciotti
    Graduate Group in Biophysics, University of California, Berkeley, California 94720, USA
    Biophys J 85:451-8. 2003
  4. ncbi Mechanism of proton transport in bacteriorhodopsin from crystallographic structures of the K, L, M1, M2, and M2' intermediates of the photocycle
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, 346D Medical Science I, Irvine, CA 92697, USA
    J Mol Biol 328:439-50. 2003
  5. ncbi Specific damage induced by X-ray radiation and structural changes in the primary photoreaction of bacteriorhodopsin
    Yasuhiro Matsui
    Graduate School of Science, Nagoya University, Chikusa, 464 8602, Nagoya, Japan
    J Mol Biol 324:469-81. 2002
  6. ncbi Three-dimensional structure of (1-71)bacterioopsin solubilized in methanol/chloroform and SDS micelles determined by 15N-1H heteronuclear NMR spectroscopy
    K V Pervushin
    Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow
    Eur J Biochem 219:571-83. 1994
  7. ncbi Protein, lipid and water organization in bacteriorhodopsin crystals: a molecular view of the purple membrane at 1.9 A resolution
    H Belrhali
    ESRF, Grenoble, France
    Structure 7:909-17. 1999
  8. pmc Electron paramagnetic resonance study of structural changes in the O photointermediate of bacteriorhodopsin
    Deliang Chen
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    J Mol Biol 366:790-805. 2007
  9. ncbi Crystallographic structure of the retinal and the protein after deprotonation of the Schiff base: the switch in the bacteriorhodopsin photocycle
    Janos Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    J Mol Biol 321:727-37. 2002
  10. ncbi Crystallographic structure of the K intermediate of bacteriorhodopsin: conservation of free energy after photoisomerization of the retinal
    Brigitte Schobert
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    J Mol Biol 321:715-26. 2002

Scientific Experts

  • J K Lanyi
  • H Luecke
  • J P Whitelegge
  • Marek Cieplak
  • S Subramaniam
  • Tsutomu Kouyama
  • Y Kimura
  • Bryan W Berger
  • Salem Faham
  • Sarah Yohannan
  • James U Bowie
  • Hideo Okumura
  • Marc T Facciotti
  • Brigitte Schobert
  • Duan Yang
  • Midori Murakami
  • E Pebay-Peyroula
  • Yasuhiro Matsui
  • A Royant
  • E M Landau
  • Deliang Chen
  • M T Facciotti
  • S Rouhani
  • Miya Kamihira
  • Taichi Nishikawa
  • Kazuki Takeda
  • Karl Edman
  • K Edman
  • Alex Perálvarez-Marín
  • H Belrhali
  • Robert M Glaeser
  • Vincent S Cheung
  • Shahab Rouhani
  • H Sato
  • Shin ichi Adachi
  • K Takeda
  • Heiko Patzelt
  • M Katragadda
  • R M Glaeser
  • H J Sass
  • R Neutze
  • K H Downing
  • P Nollert
  • K Mitsuoka
  • Jennifer M Wang
  • T Hino
  • T Okada
  • L E Ball
  • Anthony Watts
  • J P Rosenbusch
  • A S Arseniev
  • Elizabeth A Massey
  • Dawn Yang
  • Gabriella L Boulting
  • Eva Pebay-Peyroula
  • Richard Neutze
  • David van der Spoel
  • Esteve Padrós
  • Emiko Bare
  • Nitin S Baliga
  • Enric Querol
  • Mercedes Marquez
  • Tom Taylor
  • Ehud M Landau
  • Christopher S Lunde
  • Nobuo Kamiya
  • Frida Jacobson
  • José Luís Bourdelande
  • Gisela Larsson
  • Antoine Royant
  • K V Pervushin
  • Gabriella Boulting
  • Leonid S Brown
  • Doris Nguyen
  • R Henderson
  • Jill Cupp-Vickery
  • Peter Schmieder
  • Steven Smith
  • N Grigorieff
  • Bernd Simon
  • Yoshitsugu Shiro
  • Viktor Hornak
  • Keisuke Sakai
  • Dieter Oesterhelt
  • Hartmut Oschkinat
  • Brigitte Kessler
  • Antonius terLaak
  • Ronald Kühne
  • F M Betancourt
  • F T Burkard

Detail Information

Publications64

  1. ncbi Structure of bacteriorhodopsin at 1.55 A resolution
    H Luecke
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, 92697, USA
    J Mol Biol 291:899-911. 1999
    Th?e atomic structure of the light-driven ion pump bacteriorhodopsin and the surrounding lipid matrix was determined by X-ray diffraction of crystals grown in cubic lipid phase...
  2. ncbi Deformation of helix C in the low temperature L-intermediate of bacteriorhodopsin
    Karl Edman
    Department of Chemistry and Bioscience, Chalmers University of Technology, Box 462, S 40530 Gothenburg, Sweden
    J Biol Chem 279:2147-58. 2004
    ..analogues, have produced a consistent picture of the structural mechanism of light-driven proton pumping by bacteriorhodopsin. Of central importance within this picture is the structure of the L-intermediate, which follows the retinal ..
  3. pmc Crystal structure of the bromide-bound D85S mutant of bacteriorhodopsin: principles of ion pumping
    Marc T Facciotti
    Graduate Group in Biophysics, University of California, Berkeley, California 94720, USA
    Biophys J 85:451-8. 2003
    We report the crystal structure of a bromide-bound form of the D85S mutant of bacteriorhodopsin, bR(D85S), a protein that uses light energy rather than ATP to pump halide ions across the cell membrane...
  4. ncbi Mechanism of proton transport in bacteriorhodopsin from crystallographic structures of the K, L, M1, M2, and M2' intermediates of the photocycle
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, 346D Medical Science I, Irvine, CA 92697, USA
    J Mol Biol 328:439-50. 2003
    We produced the L intermediate of the photocycle in a bacteriorhodopsin crystal in photo-stationary state at 170 K with red laser illumination at 60% occupancy, and determined its structure to 1.62 A resolution...
  5. ncbi Specific damage induced by X-ray radiation and structural changes in the primary photoreaction of bacteriorhodopsin
    Yasuhiro Matsui
    Graduate School of Science, Nagoya University, Chikusa, 464 8602, Nagoya, Japan
    J Mol Biol 324:469-81. 2002
    b>Bacteriorhodopsin, the sole membrane protein of the purple membrane of Halobacterium salinarum, functions as a light-driven proton pump...
  6. ncbi Three-dimensional structure of (1-71)bacterioopsin solubilized in methanol/chloroform and SDS micelles determined by 15N-1H heteronuclear NMR spectroscopy
    K V Pervushin
    Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow
    Eur J Biochem 219:571-83. 1994
    ..Using NMR data, along with the electron cryomicroscopy model of bacteriorhodopsin [Henderson, R., Baldwin, J. M., Ceska, T. A., Zemlin, F., Beckman, E. & Downing, K. H. (1990) J. Mol...
  7. ncbi Protein, lipid and water organization in bacteriorhodopsin crystals: a molecular view of the purple membrane at 1.9 A resolution
    H Belrhali
    ESRF, Grenoble, France
    Structure 7:909-17. 1999
    b>Bacteriorhodopsin (bR) from Halobacterium salinarum is a proton pump that converts the energy of light into a proton gradient that drives ATP synthesis...
  8. pmc Electron paramagnetic resonance study of structural changes in the O photointermediate of bacteriorhodopsin
    Deliang Chen
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    J Mol Biol 366:790-805. 2007
    The structural changes of bacteriorhodopsin during its photochemical cycle, as revealed by crystal structures of trapped intermediates, have provided insights to the proton translocation mechanism...
  9. ncbi Crystallographic structure of the retinal and the protein after deprotonation of the Schiff base: the switch in the bacteriorhodopsin photocycle
    Janos Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    J Mol Biol 321:727-37. 2002
    We illuminated bacteriorhodopsin crystals at 210K to produce, in a photostationary state with 60% occupancy, the earliest M intermediate (M1) of the photocycle...
  10. ncbi Crystallographic structure of the K intermediate of bacteriorhodopsin: conservation of free energy after photoisomerization of the retinal
    Brigitte Schobert
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    J Mol Biol 321:715-26. 2002
    The K state, an early intermediate of the bacteriorhodopsin photocycle, contains the excess free energy used for light-driven proton transport...
  11. pmc The structures of the active center in dark-adapted bacteriorhodopsin by solution-state NMR spectroscopy
    Heiko Patzelt
    Max Planck Institut fur Biochemie, Abteilung Membranbiochemie, Am Klopferspitz 18a, 82152 Martinsried, Germany
    Proc Natl Acad Sci U S A 99:9765-70. 2002
    The two forms of bacteriorhodopsin present in the dark-adapted state, containing either all-trans or 13-cis,15-syn retinal, were examined by using solution state NMR, and their structures were determined...
  12. pmc The bacteriorhodopsin gene
    R Dunn
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
    Proc Natl Acad Sci U S A 78:6744-8. 1981
    The bacteriorhodopsin gene has been identified in a 5.3-kilobase restriction endonuclease fragment isolated from Halobacterium halobium DNA, using a cloned cDNA fragment as the probe...
  13. ncbi Bicelle crystallization: a new method for crystallizing membrane proteins yields a monomeric bacteriorhodopsin structure
    Salem Faham
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095 1570, USA
    J Mol Biol 316:1-6. 2002
    ..The method is flexible and simple to use. As a test case, bacteriorhodopsin (bR) from Halobacterium salinarum was crystallized from a bicellar solution, yielding a new bR crystal form...
  14. pmc Structure of an early intermediate in the M-state phase of the bacteriorhodopsin photocycle
    M T Facciotti
    Graduate Group in Biophysics, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA
    Biophys J 81:3442-55. 2001
    The structure of an early M-intermediate of the wild-type bacteriorhodopsin photocycle formed by actinic illumination at 230 K has been determined by x-ray crystallography to a resolution of 2.0 A...
  15. ncbi Crystal structure of the D85S mutant of bacteriorhodopsin: model of an O-like photocycle intermediate
    S Rouhani
    Life Sciences Division, Donner Laboratory, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
    J Mol Biol 313:615-28. 2001
    Crystal structures are reported for the D85S and D85S/F219L mutants of the light-driven proton/hydroxyl-pump bacteriorhodopsin. These mutants crystallize in the orthorhombic C222(1) spacegroup, and provide the first demonstration that ..
  16. pmc Assembly of a polytopic membrane protein structure from the solution structures of overlapping peptide fragments of bacteriorhodopsin
    M Katragadda
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269, USA
    Biophys J 81:1029-36. 2001
    ..structure of membrane proteins has been developed and tested on the integral membrane protein, bacteriorhodopsin, the crystal structure of which had previously been determined...
  17. ncbi Molecular mechanism of vectorial proton translocation by bacteriorhodopsin
    S Subramaniam
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 406:653-7. 2000
    b>Bacteriorhodopsin, a membrane protein with a relative molecular mass of 27,000, is a light driven pump which transports protons across the cell membrane of the halophilic organism Halobacterium salinarum...
  18. ncbi Crystallographic structures of the M and N intermediates of bacteriorhodopsin: assembly of a hydrogen-bonded chain of water molecules between Asp-96 and the retinal Schiff base
    Brigitte Schobert
    Department of Physiology and Biophysics, University of California, D345 Medical Science I, Irvine, CA 92697, USA
    J Mol Biol 330:553-70. 2003
    An M intermediate of wild-type bacteriorhodopsin and an N intermediate of the V49A mutant were accumulated in photostationary states at pH 5.6 and 295 K, and their crystal structures determined to 1.52A and 1.62A resolution, respectively...
  19. pmc Structural changes in the L photointermediate of bacteriorhodopsin
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    J Mol Biol 365:1379-92. 2007
    The L to M reaction of the bacteriorhodopsin photocycle includes the crucial proton transfer from the retinal Schiff base to Asp85...
  20. pmc Effects of additives on surfactant phase behavior relevant to bacteriorhodopsin crystallization
    Bryan W Berger
    Center for Molecular and Engineering Thermodynamics, Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716, USA
    Protein Sci 15:2682-96. 2006
    The interactions leading to crystallization of the integral membrane protein bacteriorhodopsin solubilized in n-octyl-beta-D-glucoside were investigated...
  21. pmc Propagating structural perturbation inside bacteriorhodopsin: crystal structures of the M state and the D96A and T46V mutants
    Janos K Lanyi
    Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
    Biochemistry 45:12003-10. 2006
    The X-ray diffraction structure of the non-illuminated D96A bacteriorhodopsin mutant reveals structural changes as far away as 15 A from residue 96, at the retinal, Trp-182, Ala-215, and waters 501, 402, and 401...
  22. ncbi Pulling single bacteriorhodopsin out of a membrane: Comparison of simulation and experiment
    Marek Cieplak
    Institute of Physics, Polish Academy of Sciences, Al Lotnikow 32 46, 02 668 Warsaw, Poland
    Biochim Biophys Acta 1758:537-44. 2006
    ..reproduces the experimentally observed differences between force-extension patterns obtained on bacteriorhodopsin at different temperatures and predicts a lack of symmetry in the choice of the terminus to pull by...
  23. ncbi Functionally relevant coupled dynamic profile of bacteriorhodopsin and lipids in purple membranes
    Miya Kamihira
    Biomembrane Structure Unit, Department of Biochemistry, Oxford University, South Parks Road, Oxford OX1 3QU, UK
    Biochemistry 45:4304-13. 2006
    The dynamics of bacteriorhodopsin (bR) and the lipid headgroups in oriented purple membranes (PMs) was determined at various temperatures and relative humidity (rh) using solid-state NMR spectroscopy...
  24. ncbi Crystal structure of the 13-cis isomer of bacteriorhodopsin in the dark-adapted state
    Taichi Nishikawa
    Department of Physics, Graduate School of Science, Nagoya University, Nagoya 464 8602, Japan
    J Mol Biol 352:319-28. 2005
    The atomic structure of the trans isomer of bacteriorhodopsin was determined previously by using a 3D crystal belonging to the space group P622...
  25. ncbi Crystal structures of acid blue and alkaline purple forms of bacteriorhodopsin
    Hideo Okumura
    Department of Physics, Graduate School of Science, Nagoya University, Nagoya 464 8602, Japan
    J Mol Biol 351:481-95. 2005
    b>Bacteriorhodopsin, a light-driven proton pump found in the purple membrane of Halobacterium salinarum, exhibits purple at neutral pH but its color is sensitive to pH...
  26. pmc Crystallization of bacteriorhodopsin from bicelle formulations at room temperature
    Salem Faham
    Department of Chemistry and Biochemistry, UCLA DOE Center for Genomics and Proteomics, Molecular Biology Institute, University of California, Los Angeles, California 90095 1570, USA
    Protein Sci 14:836-40. 2005
    We showed previously that high-quality crystals of bacteriorhodopsin (bR) from Halobacterium salinarum can be obtained from bicelle-forming DMPC/CHAPSO mixtures at 37 degrees C...
  27. ncbi Crystal structure of the M intermediate of bacteriorhodopsin: allosteric structural changes mediated by sliding movement of a transmembrane helix
    Kazuki Takeda
    Department of Physics, Graduate School of Science, Nagoya University, Japan
    J Mol Biol 341:1023-37. 2004
    Structural changes in the proton pumping cycle of wild-type bacteriorhodopsin were investigated by using a 3D crystal (space group P622)prepared by the membrane fusion method...
  28. ncbi Proline substitutions are not easily accommodated in a membrane protein
    Sarah Yohannan
    Department of Chemistry and Biochemistry, DOE Center for Genomics and Proteomics, Molecular Biology Institute, 655 Boyer Hall, 611 Charles E Young Dr E, University of California, Los Angeles, Los Angeles, CA 90095 1570, USA
    J Mol Biol 341:1-6. 2004
    ..tested this possibility by making proline substitutions at 15 positions throughout the N-terminal half of bacteriorhodopsin helix B...
  29. ncbi Specificity of anion binding in the substrate pocket of bacteriorhodopsin
    Marc T Facciotti
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Biochemistry 43:4934-43. 2004
    The structure of the D85S mutant of bacteriorhodopsin with a nitrate anion bound in the Schiff base binding site and the structure of the anion-free protein have been obtained in the same crystal form...
  30. pmc The evolution of transmembrane helix kinks and the structural diversity of G protein-coupled receptors
    Sarah Yohannan
    Department of Chemistry and Biochemistry, UCLA DOE Institute for Genomics and Proteomics, Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 101:959-63. 2004
    ..We observe, however, that the three prolines in bacteriorhodopsin transmembrane helices can be changed to alanine with little structural consequences...
  31. ncbi Crystal structure of the L intermediate of bacteriorhodopsin: evidence for vertical translocation of a water molecule during the proton pumping cycle
    Tsutomu Kouyama
    Department of Physics, Graduate School of Science, Nagoya University, Furo cho, Chikusa, Nagoya 464 8602, Japan
    J Mol Biol 335:531-46. 2004
    For structural investigation of the L intermediate of bacteriorhodopsin, a 3D crystal belonging to the space group P622 was illuminated with green light at 160 K and subsequently with red light at 100 K...
  32. ncbi Side-chain contributions to membrane protein structure and stability
    Salem Faham
    Department of Chemistry and Biochemistry, UCLA DOE Center for Genomics and Proteomics, Molecular Biology Institute, University of California, Los Angeles, CA 90095 1570, USA
    J Mol Biol 335:297-305. 2004
    ..To investigate these forces we introduced alanine substitutions at 24 positions in the B helix of bacteriorhodopsin and examined their effects on structure and stability...
  33. ncbi Structural alterations for proton translocation in the M state of wild-type bacteriorhodopsin
    H J Sass
    Institute of Structural Biology, Research Centre Julich, Germany
    Nature 406:649-53. 2000
    ..The light-driven proton pump bacteriorhodopsin is the best-characterized protein providing this function...
  34. ncbi Helix deformation is coupled to vectorial proton transport in the photocycle of bacteriorhodopsin
    A Royant
    Institut de Biologie Structurale, CEA CNRS Université Joseph Fourier, UMR 5075, Grenoble, France
    Nature 406:645-8. 2000
    ..b>Bacteriorhodopsin, the simplest known proton pump, provides a paradigm for understanding this process. Here we report, at 2...
  35. pmc Attachment site(s) of retinal in bacteriorhodopsin
    N V Katre
    Proc Natl Acad Sci U S A 78:4068-72. 1981
    After chemical reduction of the retinylidene-lysine Schiff base linkage in bacteriorhodopsin, the retinyl residue is covalently attached to Lys-216 (with a possible minor fraction on Lys-172) or to both Lys-216(172) and Lys-40/41...
  36. ncbi Bacteriorhodopsin precursor. Characterization and its integration into the purple membrane
    J S Seehra
    J Biol Chem 259:4187-93. 1984
    Halobacterium halobium spheroplasts synthesize and accumulate a bacteriorhodopsin precursor...
  37. ncbi Studies on the light-transducing pigment bacteriorhodopsin
    R J Dunn
    Cold Spring Harb Symp Quant Biol 48:853-62. 1983
  38. ncbi Tertiary structure of bacteriorhodopsin. Positions and orientations of helices A and B in the structural map determined by neutron diffraction
    J L Popot
    Institut de Biologie Physico Chimique, Paris, France
    J Mol Biol 210:829-47. 1989
    Positions and rotations of two helices in the tertiary structure of bacteriorhodopsin have been studied by neutron diffraction using reconstituted, hybrid purple membrane samples...
  39. ncbi Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy
    R Henderson
    MRC Laboratory of Molecular Biology, Cambridge, U K
    J Mol Biol 213:899-929. 1990
    The light-driven proton pump bacteriorhodopsin occurs naturally as two-dimensional crystals...
  40. pmc Bacterioopsin, haloopsin, and sensory opsin I of the halobacterial isolate Halobacterium sp. strain SG1: three new members of a growing family
    J Soppa
    Max Planck Institut fur Biochemie, Martinsried, Germany
    J Bacteriol 175:2720-6. 1993
    ..These results were in excellent agreement with the structural model of bacteriorhodopsin of Halobacterium halobium as well as with mutant studies, indicating that (i) structure predictions based on ..
  41. ncbi Sequence-specific 1H-NMR assignment and conformation of proteolytic fragment 163-231 of bacterioopsin
    I L Barsukov
    Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences
    Eur J Biochem 192:321-7. 1990
    ....
  42. ncbi Three-dimensional structure of proteolytic fragment 163-231 of bacterioopsin determined from nuclear magnetic resonance data in solution
    I L Barsukov
    Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of Russia, Moscow
    Eur J Biochem 206:665-72. 1992
    ..The alpha-helices correspond to transmembrane segments F and G of bacteriorhodopsin. The segment F contains proline 186, which introduces a kink of about 25 degrees with a disruption of the ..
  43. ncbi Spatial structure of (34-65)bacterioopsin polypeptide in SDS micelles determined from nuclear magnetic resonance data
    A L Lomize
    M M Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow
    J Biomol NMR 2:361-72. 1992
    ..The secondary structure of segment B in micelles is consistent with the high-resolution electron cryomicroscopy model of bacteriorhodopsin (Henderson et al. (1990) J. Mol. Biol., 213, 899-929).
  44. ncbi Sequence-specific resonance assignment and secondary structure of (1-71) bacterioopsin
    A G Sobol
    Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow
    J Biomol NMR 2:161-71. 1992
    ..The N-terminal part of the fragment (Ala2-Gly6) adopts the helical conformation stabilized by 3 hydrogen bonds...
  45. ncbi 1H-15N-NMR studies of bacteriorhodopsin Halobacterium halobium. Conformational dynamics of the four-helical bundle
    - Orekhov VYu
    Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow
    Eur J Biochem 210:223-9. 1992
    ..Less than half of the cross-peaks expected from the amino acid sequence of uniformly 15N-labeled bacteriorhodopsin were observed, using heteronuclear 1H-15N coherence spectroscopy...
  46. ncbi Three-dimensional structure of (1-36)bacterioopsin in methanol-chloroform mixture and SDS micelles determined by 2D 1H-NMR spectroscopy
    K V Pervushin
    Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow
    FEBS Lett 308:190-6. 1992
    ..heavy atoms and fit well with Pro8 to Met32 alpha-helical region in electron cryo-microscopy model of bacteriorhodopsin. The N-terminal region Ala2-Gly6 of sA in organic mixture has a fixed structure of two consecutive gamma-..
  47. ncbi Three-dimensional model of purple membrane obtained by electron microscopy
    R Henderson
    Nature 257:28-32. 1975
    ..Lipid bilayer regions fill the spaces between the protein molecules...
  48. ncbi Orthorhombic crystal form of bacteriorhodopsin nucleated on benzamidine diffracting to 3.6 A resolution
    G F Schertler
    Max Planck Institut fur Biochemie, Martinsried, Germany
    J Mol Biol 234:156-64. 1993
    ..Freshly formed benzamidine crystals were found to provide a suitable nucleation surface for crystallisation of bacteriorhodopsin. At 20 degrees C and 1% octylglucoside pseudohexagonal needles of bacteriorhodopsin nucleated on the ..
  49. ncbi Specific labelling of the protein and lipid on the extracellular surface of purple membrane
    R Henderson
    J Mol Biol 123:259-74. 1978
  50. ncbi Coupling photoisomerization of retinal to directional transport in bacteriorhodopsin
    H Luecke
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    J Mol Biol 300:1237-55. 2000
    In order to understand how isomerization of the retinal drives unidirectional transmembrane ion transport in bacteriorhodopsin, we determined the atomic structures of the BR state and M photointermediate of the E204Q mutant, to 1.7 and 1...
  51. ncbi High-resolution X-ray structure of an early intermediate in the bacteriorhodopsin photocycle
    K Edman
    Department of Biochemistry, Uppsala University, Biomedical Centre, Sweden
    Nature 401:822-6. 1999
    b>Bacteriorhodopsin is the simplest known photon-driven proton pump and as such provides a model for the study of a basic function in bioenergetics...
  52. ncbi Structural changes in bacteriorhodopsin during ion transport at 2 angstrom resolution
    H Luecke
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    Science 286:255-61. 1999
    Crystal structures of the Asp96 to Asn mutant of the light-driven proton pump bacteriorhodopsin and its M photointermediate produced by illumination at ambient temperature have been determined to 1.8 and 2...
  53. ncbi Specific lipid-protein interactions in a novel honeycomb lattice structure of bacteriorhodopsin
    H Sato
    Department of Physics, Graduate School of Science, Nagoya University, Nagoya 464 8602, Japan
    Acta Crystallogr D Biol Crystallogr 55:1251-6. 1999
    In the purple membrane of Halobacterium salinarium, bacteriorhodopsin trimers are arranged in a hexagonal lattice...
  54. ncbi The structure of bacteriorhodopsin at 3.0 A resolution based on electron crystallography: implication of the charge distribution
    K Mitsuoka
    Department of Biophysics Faculty of Science, Kyoto University, Oiwake cho Kitashirakawa, Sakyo ku, Kyoto, 606 8502, Japan
    J Mol Biol 286:861-82. 1999
    ..work, we observed two different types of densities around acidic residues in the experimental (|Fo|) map of bacteriorhodopsin (bR), a light-driven proton pump...
  55. ncbi A novel three-dimensional crystal of bacteriorhodopsin obtained by successive fusion of the vesicular assemblies
    K Takeda
    Graduate School of Science, Nagoya University, Nagoya, 464 8602, Japan
    J Mol Biol 283:463-74. 1998
    When the two-dimensional crystal of bacteriorhodopsin (bR), purple membrane, is incubated at high temperature (32 degreesC) with a small amount of the neutral detergent octylthioglucoside in the presence of the precipitant ammonium ..
  56. pmc Electrospray-ionization mass spectrometry of intact intrinsic membrane proteins
    J P Whitelegge
    Center for Molecular and Medical Sciences Mass Spectrometry, Department of Chemistry and Biochemistry, University of California, Los Angeles 90095 1569, USA
    Protein Sci 7:1423-30. 1998
    ..The spectra of four examples, bacteriorhodopsin and its apoprotein from Halobacterium and the D1 and D2 reaction-center subunits from spinach thylakoids, ..
  57. ncbi Proton transfer pathways in bacteriorhodopsin at 2.3 angstrom resolution
    H Luecke
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    Science 280:1934-7. 1998
    Photoisomerization of the retinal of bacteriorhodopsin initiates a cyclic reaction in which a proton is translocated across the membrane. Studies of this protein promise a better understanding of how ion pumps function...
  58. pmc Mass spectrometric analysis of integral membrane proteins: application to complete mapping of bacteriorhodopsins and rhodopsin
    L E Ball
    Department of Pharmacology, Medical University of South Carolina, Charleston 29425, USA
    Protein Sci 7:758-64. 1998
    ..This method has been applied to wild type (WT) bacteriorhodopsin (bR), cysteine containing mutants of bR, and the prototypical G-protein coupled receptor, rhodopsin (Rh)...
  59. ncbi Surface of bacteriorhodopsin revealed by high-resolution electron crystallography
    Y Kimura
    Biomolecular Engineering Research Institute, Osaka, Japan
    Nature 389:206-11. 1997
    b>Bacteriorhodopsin is a transmembrane protein that uses light energy, absorbed by its chromophore retinal, to pump protons from the cytoplasm of bacteria such as Halobacterium salinarium into the extracellular space...
  60. ncbi X-ray structure of bacteriorhodopsin at 2.5 angstroms from microcrystals grown in lipidic cubic phases
    E Pebay-Peyroula
    Institut de Biologie Structurale CEA CNRS Université Joseph Fourier, 41 Avenue des Martyrs, F 38027 Grenoble Cedex 1, France
    Science 277:1676-81. 1997
    ..cubic phases provide a continuous three-dimensional bilayer matrix that facilitates nucleation and growth of bacteriorhodopsin microcrystals. The crystals diffract x-rays isotropically to 2.0 angstroms...
  61. ncbi Electron-crystallographic refinement of the structure of bacteriorhodopsin
    N Grigorieff
    MRC Laboratory of Molecular Biology, Cambridge, UK
    J Mol Biol 259:393-421. 1996
    ..from 30 new images of tilted specimens, an improved experimental density map has been calculated for bacteriorhodopsin. The atomic model has then been rebuilt into this new map with particular attention to the surface loops...
  62. ncbi Thr-90 plays a vital role in the structure and function of bacteriorhodopsin
    Alex Perálvarez-Marín
    Unitat de Biofisica, Departament de Bioquimica i de Biologia Molecular, Facultat de Medicina, Universitat Autonoma de Barcelona, Bellaterra Cerdanyola del Valles, Barcelona, Spain
    J Biol Chem 279:16403-9. 2004
    The role of Thr-90 in the bacteriorhodopsin structure and function was investigated by its replacement with Ala and Val. The mutant D115A was also studied because Asp-115 in helix D forms a hydrogen bond with Thr-90 in helix C...
  63. ncbi Analysis of high-resolution electron diffraction patterns from purple membrane labelled with heavy-atoms
    T A Ceska
    MRC Laboratory of Molecular Biology, Cambridge, England
    J Mol Biol 213:539-60. 1990
    Progress in the structure determination of bacteriorhodopsin, the protein component of purple membrane from Halobacterium halobium has been limited by the lack of three-dimensional phase information between 6 and 3 A resolution...

Research Grants24

  1. STRUCTURAL STUDIES OF THE BACTERIORHODOPSIN PHOTOCYCLE
    Hartmut Luecke; Fiscal Year: 2002
    DESCRIPTION (Adapted from abstract): Bacteriorhodopsin is a light-driven proton pump that has several well-characterized intermediates in its photocycle...
  2. LIGHT-DRIVEN ION TRANSPORT IN BACTERIAL RHODOPSINS
    Janos Lanyi; Fiscal Year: 2007
    b>Bacteriorhodopsin and halorhodopsin are the simplest ion pumps, and prototypes of the ubiquitous seven-transmembrane-helical proteins...
  3. Structure-Function Studies of the Proton-Gated Urea Channel from H. Pylori
    Hartmut Luecke; Fiscal Year: 2009
    ....
  4. Structure-Function Studies of Annexin I and II
    Hartmut Luecke; Fiscal Year: 2006
    ..5) Co-crystallize annexin with t-PA and solve the structure of this complex after characterizing complex formation in solution. ..
  5. The mechanism of signal transduction in photoreceptors
    Hartmut Luecke; Fiscal Year: 2006
    ..5. Determine the crystal structure of the primary Anabaena photoreceptor in the ground and signaling state. 6. Determine the structure of the complex of the Anabaena photoreceptor with its putative transducer. ..
  6. Structure-Function Studies of the Proton-Gated Urea Channel from H. Pylori
    Hartmut Luecke; Fiscal Year: 2010
    ....
  7. Structure-Function Studies of the Proton-Gated Urea Channel from H. Pylori
    Hartmut Luecke; Fiscal Year: 2009
    ....