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| bacteriorhodopsinSummaryGene Symbol: bacteriorhodopsin Description: bacterio-opsin Species: Halobacterium sp. NRC-1 Top Publications
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Structure of bacteriorhodopsin at 1.55 A resolutionH Luecke
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, 92697, USA
J Mol Biol 291:899-911. 1999Th?e atomic structure of the light-driven ion pump bacteriorhodopsin and the surrounding lipid matrix was determined by X-ray diffraction of crystals grown in cubic lipid phase...- Deformation of helix C in the low temperature L-intermediate of bacteriorhodopsinKarl Edman
Department of Chemistry and Bioscience, Chalmers University of Technology, Box 462, S 40530 Gothenburg, Sweden
J Biol Chem 279:2147-58. 2004..analogues, have produced a consistent picture of the structural mechanism of light-driven proton pumping by bacteriorhodopsin. Of central importance within this picture is the structure of the L-intermediate, which follows the retinal .. 
Crystal structure of the bromide-bound D85S mutant of bacteriorhodopsin: principles of ion pumpingMarc T Facciotti
Graduate Group in Biophysics, University of California, Berkeley, California 94720, USA
Biophys J 85:451-8. 2003We report the crystal structure of a bromide-bound form of the D85S mutant of bacteriorhodopsin, bR(D85S), a protein that uses light energy rather than ATP to pump halide ions across the cell membrane...- Mechanism of proton transport in bacteriorhodopsin from crystallographic structures of the K, L, M1, M2, and M2' intermediates of the photocycleJanos K Lanyi
Department of Physiology and Biophysics, University of California, 346D Medical Science I, Irvine, CA 92697, USA
J Mol Biol 328:439-50. 2003We produced the L intermediate of the photocycle in a bacteriorhodopsin crystal in photo-stationary state at 170 K with red laser illumination at 60% occupancy, and determined its structure to 1.62 A resolution... - Specific damage induced by X-ray radiation and structural changes in the primary photoreaction of bacteriorhodopsinYasuhiro Matsui
Graduate School of Science, Nagoya University, Chikusa, 464 8602, Nagoya, Japan
J Mol Biol 324:469-81. 2002b>Bacteriorhodopsin, the sole membrane protein of the purple membrane of Halobacterium salinarum, functions as a light-driven proton pump... - Three-dimensional structure of (1-71)bacterioopsin solubilized in methanol/chloroform and SDS micelles determined by 15N-1H heteronuclear NMR spectroscopyK V Pervushin
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow
Eur J Biochem 219:571-83. 1994..Using NMR data, along with the electron cryomicroscopy model of bacteriorhodopsin [Henderson, R., Baldwin, J. M., Ceska, T. A., Zemlin, F., Beckman, E. & Downing, K. H. (1990) J. Mol... - Protein, lipid and water organization in bacteriorhodopsin crystals: a molecular view of the purple membrane at 1.9 A resolutionH Belrhali
ESRF, Grenoble, France
Structure 7:909-17. 1999b>Bacteriorhodopsin (bR) from Halobacterium salinarum is a proton pump that converts the energy of light into a proton gradient that drives ATP synthesis... - Electron paramagnetic resonance study of structural changes in the O photointermediate of bacteriorhodopsinDeliang Chen
Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
J Mol Biol 366:790-805. 2007The structural changes of bacteriorhodopsin during its photochemical cycle, as revealed by crystal structures of trapped intermediates, have provided insights to the proton translocation mechanism... - Crystallographic structure of the retinal and the protein after deprotonation of the Schiff base: the switch in the bacteriorhodopsin photocycleJanos Lanyi
Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
J Mol Biol 321:727-37. 2002We illuminated bacteriorhodopsin crystals at 210K to produce, in a photostationary state with 60% occupancy, the earliest M intermediate (M1) of the photocycle... - Crystallographic structure of the K intermediate of bacteriorhodopsin: conservation of free energy after photoisomerization of the retinalBrigitte Schobert
Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
J Mol Biol 321:715-26. 2002The K state, an early intermediate of the bacteriorhodopsin photocycle, contains the excess free energy used for light-driven proton transport... - The structures of the active center in dark-adapted bacteriorhodopsin by solution-state NMR spectroscopyHeiko Patzelt
Max Planck Institut fur Biochemie, Abteilung Membranbiochemie, Am Klopferspitz 18a, 82152 Martinsried, Germany
Proc Natl Acad Sci U S A 99:9765-70. 2002The two forms of bacteriorhodopsin present in the dark-adapted state, containing either all-trans or 13-cis,15-syn retinal, were examined by using solution state NMR, and their structures were determined... - The bacteriorhodopsin geneR Dunn
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Proc Natl Acad Sci U S A 78:6744-8. 1981The bacteriorhodopsin gene has been identified in a 5.3-kilobase restriction endonuclease fragment isolated from Halobacterium halobium DNA, using a cloned cDNA fragment as the probe... - Bicelle crystallization: a new method for crystallizing membrane proteins yields a monomeric bacteriorhodopsin structureSalem Faham
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095 1570, USA
J Mol Biol 316:1-6. 2002..The method is flexible and simple to use. As a test case, bacteriorhodopsin (bR) from Halobacterium salinarum was crystallized from a bicellar solution, yielding a new bR crystal form... - Structure of an early intermediate in the M-state phase of the bacteriorhodopsin photocycleM T Facciotti
Graduate Group in Biophysics, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA
Biophys J 81:3442-55. 2001The structure of an early M-intermediate of the wild-type bacteriorhodopsin photocycle formed by actinic illumination at 230 K has been determined by x-ray crystallography to a resolution of 2.0 A... - Crystal structure of the D85S mutant of bacteriorhodopsin: model of an O-like photocycle intermediateS Rouhani
Life Sciences Division, Donner Laboratory, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
J Mol Biol 313:615-28. 2001Crystal structures are reported for the D85S and D85S/F219L mutants of the light-driven proton/hydroxyl-pump bacteriorhodopsin. These mutants crystallize in the orthorhombic C222(1) spacegroup, and provide the first demonstration that .. - Assembly of a polytopic membrane protein structure from the solution structures of overlapping peptide fragments of bacteriorhodopsinM Katragadda
Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269, USA
Biophys J 81:1029-36. 2001..structure of membrane proteins has been developed and tested on the integral membrane protein, bacteriorhodopsin, the crystal structure of which had previously been determined... - Molecular mechanism of vectorial proton translocation by bacteriorhodopsinS Subramaniam
MRC Laboratory of Molecular Biology, Cambridge, UK
Nature 406:653-7. 2000b>Bacteriorhodopsin, a membrane protein with a relative molecular mass of 27,000, is a light driven pump which transports protons across the cell membrane of the halophilic organism Halobacterium salinarum... - Crystallographic structures of the M and N intermediates of bacteriorhodopsin: assembly of a hydrogen-bonded chain of water molecules between Asp-96 and the retinal Schiff baseBrigitte Schobert
Department of Physiology and Biophysics, University of California, D345 Medical Science I, Irvine, CA 92697, USA
J Mol Biol 330:553-70. 2003An M intermediate of wild-type bacteriorhodopsin and an N intermediate of the V49A mutant were accumulated in photostationary states at pH 5.6 and 295 K, and their crystal structures determined to 1.52A and 1.62A resolution, respectively... - Structural changes in the L photointermediate of bacteriorhodopsinJanos K Lanyi
Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
J Mol Biol 365:1379-92. 2007The L to M reaction of the bacteriorhodopsin photocycle includes the crucial proton transfer from the retinal Schiff base to Asp85... - Effects of additives on surfactant phase behavior relevant to bacteriorhodopsin crystallizationBryan W Berger
Center for Molecular and Engineering Thermodynamics, Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716, USA
Protein Sci 15:2682-96. 2006The interactions leading to crystallization of the integral membrane protein bacteriorhodopsin solubilized in n-octyl-beta-D-glucoside were investigated... - Propagating structural perturbation inside bacteriorhodopsin: crystal structures of the M state and the D96A and T46V mutantsJanos K Lanyi
Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA
Biochemistry 45:12003-10. 2006The X-ray diffraction structure of the non-illuminated D96A bacteriorhodopsin mutant reveals structural changes as far away as 15 A from residue 96, at the retinal, Trp-182, Ala-215, and waters 501, 402, and 401... - Pulling single bacteriorhodopsin out of a membrane: Comparison of simulation and experimentMarek Cieplak
Institute of Physics, Polish Academy of Sciences, Al Lotnikow 32 46, 02 668 Warsaw, Poland
Biochim Biophys Acta 1758:537-44. 2006..reproduces the experimentally observed differences between force-extension patterns obtained on bacteriorhodopsin at different temperatures and predicts a lack of symmetry in the choice of the terminus to pull by... - Functionally relevant coupled dynamic profile of bacteriorhodopsin and lipids in purple membranesMiya Kamihira
Biomembrane Structure Unit, Department of Biochemistry, Oxford University, South Parks Road, Oxford OX1 3QU, UK
Biochemistry 45:4304-13. 2006The dynamics of bacteriorhodopsin (bR) and the lipid headgroups in oriented purple membranes (PMs) was determined at various temperatures and relative humidity (rh) using solid-state NMR spectroscopy... - Crystal structure of the 13-cis isomer of bacteriorhodopsin in the dark-adapted stateTaichi Nishikawa
Department of Physics, Graduate School of Science, Nagoya University, Nagoya 464 8602, Japan
J Mol Biol 352:319-28. 2005The atomic structure of the trans isomer of bacteriorhodopsin was determined previously by using a 3D crystal belonging to the space group P622... - Crystal structures of acid blue and alkaline purple forms of bacteriorhodopsinHideo Okumura
Department of Physics, Graduate School of Science, Nagoya University, Nagoya 464 8602, Japan
J Mol Biol 351:481-95. 2005b>Bacteriorhodopsin, a light-driven proton pump found in the purple membrane of Halobacterium salinarum, exhibits purple at neutral pH but its color is sensitive to pH... - Crystallization of bacteriorhodopsin from bicelle formulations at room temperatureSalem Faham
Department of Chemistry and Biochemistry, UCLA DOE Center for Genomics and Proteomics, Molecular Biology Institute, University of California, Los Angeles, California 90095 1570, USA
Protein Sci 14:836-40. 2005We showed previously that high-quality crystals of bacteriorhodopsin (bR) from Halobacterium salinarum can be obtained from bicelle-forming DMPC/CHAPSO mixtures at 37 degrees C... - Crystal structure of the M intermediate of bacteriorhodopsin: allosteric structural changes mediated by sliding movement of a transmembrane helixKazuki Takeda
Department of Physics, Graduate School of Science, Nagoya University, Japan
J Mol Biol 341:1023-37. 2004Structural changes in the proton pumping cycle of wild-type bacteriorhodopsin were investigated by using a 3D crystal (space group P622)prepared by the membrane fusion method... - Proline substitutions are not easily accommodated in a membrane proteinSarah Yohannan
Department of Chemistry and Biochemistry, DOE Center for Genomics and Proteomics, Molecular Biology Institute, 655 Boyer Hall, 611 Charles E Young Dr E, University of California, Los Angeles, Los Angeles, CA 90095 1570, USA
J Mol Biol 341:1-6. 2004..tested this possibility by making proline substitutions at 15 positions throughout the N-terminal half of bacteriorhodopsin helix B... - Specificity of anion binding in the substrate pocket of bacteriorhodopsinMarc T Facciotti
Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
Biochemistry 43:4934-43. 2004The structure of the D85S mutant of bacteriorhodopsin with a nitrate anion bound in the Schiff base binding site and the structure of the anion-free protein have been obtained in the same crystal form... - The evolution of transmembrane helix kinks and the structural diversity of G protein-coupled receptorsSarah Yohannan
Department of Chemistry and Biochemistry, UCLA DOE Institute for Genomics and Proteomics, Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
Proc Natl Acad Sci U S A 101:959-63. 2004..We observe, however, that the three prolines in bacteriorhodopsin transmembrane helices can be changed to alanine with little structural consequences... - Crystal structure of the L intermediate of bacteriorhodopsin: evidence for vertical translocation of a water molecule during the proton pumping cycleTsutomu Kouyama
Department of Physics, Graduate School of Science, Nagoya University, Furo cho, Chikusa, Nagoya 464 8602, Japan
J Mol Biol 335:531-46. 2004For structural investigation of the L intermediate of bacteriorhodopsin, a 3D crystal belonging to the space group P622 was illuminated with green light at 160 K and subsequently with red light at 100 K... - Side-chain contributions to membrane protein structure and stabilitySalem Faham
Department of Chemistry and Biochemistry, UCLA DOE Center for Genomics and Proteomics, Molecular Biology Institute, University of California, Los Angeles, CA 90095 1570, USA
J Mol Biol 335:297-305. 2004..To investigate these forces we introduced alanine substitutions at 24 positions in the B helix of bacteriorhodopsin and examined their effects on structure and stability... - Structural alterations for proton translocation in the M state of wild-type bacteriorhodopsinH J Sass
Institute of Structural Biology, Research Centre Julich, Germany
Nature 406:649-53. 2000..The light-driven proton pump bacteriorhodopsin is the best-characterized protein providing this function... - Helix deformation is coupled to vectorial proton transport in the photocycle of bacteriorhodopsinA Royant
Institut de Biologie Structurale, CEA CNRS Université Joseph Fourier, UMR 5075, Grenoble, France
Nature 406:645-8. 2000..b>Bacteriorhodopsin, the simplest known proton pump, provides a paradigm for understanding this process. Here we report, at 2... - Attachment site(s) of retinal in bacteriorhodopsinN V Katre
Proc Natl Acad Sci U S A 78:4068-72. 1981After chemical reduction of the retinylidene-lysine Schiff base linkage in bacteriorhodopsin, the retinyl residue is covalently attached to Lys-216 (with a possible minor fraction on Lys-172) or to both Lys-216(172) and Lys-40/41... - Bacteriorhodopsin precursor. Characterization and its integration into the purple membraneJ S Seehra
J Biol Chem 259:4187-93. 1984Halobacterium halobium spheroplasts synthesize and accumulate a bacteriorhodopsin precursor... - Studies on the light-transducing pigment bacteriorhodopsinR J Dunn
Cold Spring Harb Symp Quant Biol 48:853-62. 1983 - Tertiary structure of bacteriorhodopsin. Positions and orientations of helices A and B in the structural map determined by neutron diffractionJ L Popot
Institut de Biologie Physico Chimique, Paris, France
J Mol Biol 210:829-47. 1989Positions and rotations of two helices in the tertiary structure of bacteriorhodopsin have been studied by neutron diffraction using reconstituted, hybrid purple membrane samples... - Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopyR Henderson
MRC Laboratory of Molecular Biology, Cambridge, U K
J Mol Biol 213:899-929. 1990The light-driven proton pump bacteriorhodopsin occurs naturally as two-dimensional crystals... - Bacterioopsin, haloopsin, and sensory opsin I of the halobacterial isolate Halobacterium sp. strain SG1: three new members of a growing familyJ Soppa
Max Planck Institut fur Biochemie, Martinsried, Germany
J Bacteriol 175:2720-6. 1993..These results were in excellent agreement with the structural model of bacteriorhodopsin of Halobacterium halobium as well as with mutant studies, indicating that (i) structure predictions based on .. - Sequence-specific 1H-NMR assignment and conformation of proteolytic fragment 163-231 of bacterioopsinI L Barsukov
Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences
Eur J Biochem 192:321-7. 1990.... - Three-dimensional structure of proteolytic fragment 163-231 of bacterioopsin determined from nuclear magnetic resonance data in solutionI L Barsukov
Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of Russia, Moscow
Eur J Biochem 206:665-72. 1992..The alpha-helices correspond to transmembrane segments F and G of bacteriorhodopsin. The segment F contains proline 186, which introduces a kink of about 25 degrees with a disruption of the .. - Spatial structure of (34-65)bacterioopsin polypeptide in SDS micelles determined from nuclear magnetic resonance dataA L Lomize
M M Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow
J Biomol NMR 2:361-72. 1992..The secondary structure of segment B in micelles is consistent with the high-resolution electron cryomicroscopy model of bacteriorhodopsin (Henderson et al. (1990) J. Mol. Biol., 213, 899-929). - Sequence-specific resonance assignment and secondary structure of (1-71) bacterioopsinA G Sobol
Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow
J Biomol NMR 2:161-71. 1992..The N-terminal part of the fragment (Ala2-Gly6) adopts the helical conformation stabilized by 3 hydrogen bonds... - 1H-15N-NMR studies of bacteriorhodopsin Halobacterium halobium. Conformational dynamics of the four-helical bundle- Orekhov VYu
Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow
Eur J Biochem 210:223-9. 1992..Less than half of the cross-peaks expected from the amino acid sequence of uniformly 15N-labeled bacteriorhodopsin were observed, using heteronuclear 1H-15N coherence spectroscopy... - Three-dimensional structure of (1-36)bacterioopsin in methanol-chloroform mixture and SDS micelles determined by 2D 1H-NMR spectroscopyK V Pervushin
Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow
FEBS Lett 308:190-6. 1992..heavy atoms and fit well with Pro8 to Met32 alpha-helical region in electron cryo-microscopy model of bacteriorhodopsin. The N-terminal region Ala2-Gly6 of sA in organic mixture has a fixed structure of two consecutive gamma-.. - Three-dimensional model of purple membrane obtained by electron microscopyR Henderson
Nature 257:28-32. 1975..Lipid bilayer regions fill the spaces between the protein molecules... - Orthorhombic crystal form of bacteriorhodopsin nucleated on benzamidine diffracting to 3.6 A resolutionG F Schertler
Max Planck Institut fur Biochemie, Martinsried, Germany
J Mol Biol 234:156-64. 1993..Freshly formed benzamidine crystals were found to provide a suitable nucleation surface for crystallisation of bacteriorhodopsin. At 20 degrees C and 1% octylglucoside pseudohexagonal needles of bacteriorhodopsin nucleated on the .. - Specific labelling of the protein and lipid on the extracellular surface of purple membraneR Henderson
J Mol Biol 123:259-74. 1978 - Coupling photoisomerization of retinal to directional transport in bacteriorhodopsinH Luecke
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
J Mol Biol 300:1237-55. 2000In order to understand how isomerization of the retinal drives unidirectional transmembrane ion transport in bacteriorhodopsin, we determined the atomic structures of the BR state and M photointermediate of the E204Q mutant, to 1.7 and 1... - High-resolution X-ray structure of an early intermediate in the bacteriorhodopsin photocycleK Edman
Department of Biochemistry, Uppsala University, Biomedical Centre, Sweden
Nature 401:822-6. 1999b>Bacteriorhodopsin is the simplest known photon-driven proton pump and as such provides a model for the study of a basic function in bioenergetics... - Structural changes in bacteriorhodopsin during ion transport at 2 angstrom resolutionH Luecke
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
Science 286:255-61. 1999Crystal structures of the Asp96 to Asn mutant of the light-driven proton pump bacteriorhodopsin and its M photointermediate produced by illumination at ambient temperature have been determined to 1.8 and 2... - Specific lipid-protein interactions in a novel honeycomb lattice structure of bacteriorhodopsinH Sato
Department of Physics, Graduate School of Science, Nagoya University, Nagoya 464 8602, Japan
Acta Crystallogr D Biol Crystallogr 55:1251-6. 1999In the purple membrane of Halobacterium salinarium, bacteriorhodopsin trimers are arranged in a hexagonal lattice... - The structure of bacteriorhodopsin at 3.0 A resolution based on electron crystallography: implication of the charge distributionK Mitsuoka
Department of Biophysics Faculty of Science, Kyoto University, Oiwake cho Kitashirakawa, Sakyo ku, Kyoto, 606 8502, Japan
J Mol Biol 286:861-82. 1999..work, we observed two different types of densities around acidic residues in the experimental (|Fo|) map of bacteriorhodopsin (bR), a light-driven proton pump... - A novel three-dimensional crystal of bacteriorhodopsin obtained by successive fusion of the vesicular assembliesK Takeda
Graduate School of Science, Nagoya University, Nagoya, 464 8602, Japan
J Mol Biol 283:463-74. 1998When the two-dimensional crystal of bacteriorhodopsin (bR), purple membrane, is incubated at high temperature (32 degreesC) with a small amount of the neutral detergent octylthioglucoside in the presence of the precipitant ammonium .. - Electrospray-ionization mass spectrometry of intact intrinsic membrane proteinsJ P Whitelegge
Center for Molecular and Medical Sciences Mass Spectrometry, Department of Chemistry and Biochemistry, University of California, Los Angeles 90095 1569, USA
Protein Sci 7:1423-30. 1998..The spectra of four examples, bacteriorhodopsin and its apoprotein from Halobacterium and the D1 and D2 reaction-center subunits from spinach thylakoids, .. - Proton transfer pathways in bacteriorhodopsin at 2.3 angstrom resolutionH Luecke
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
Science 280:1934-7. 1998Photoisomerization of the retinal of bacteriorhodopsin initiates a cyclic reaction in which a proton is translocated across the membrane. Studies of this protein promise a better understanding of how ion pumps function... - Mass spectrometric analysis of integral membrane proteins: application to complete mapping of bacteriorhodopsins and rhodopsinL E Ball
Department of Pharmacology, Medical University of South Carolina, Charleston 29425, USA
Protein Sci 7:758-64. 1998..This method has been applied to wild type (WT) bacteriorhodopsin (bR), cysteine containing mutants of bR, and the prototypical G-protein coupled receptor, rhodopsin (Rh)... - Surface of bacteriorhodopsin revealed by high-resolution electron crystallographyY Kimura
Biomolecular Engineering Research Institute, Osaka, Japan
Nature 389:206-11. 1997b>Bacteriorhodopsin is a transmembrane protein that uses light energy, absorbed by its chromophore retinal, to pump protons from the cytoplasm of bacteria such as Halobacterium salinarium into the extracellular space... - X-ray structure of bacteriorhodopsin at 2.5 angstroms from microcrystals grown in lipidic cubic phasesE Pebay-Peyroula
Institut de Biologie Structurale CEA CNRS Université Joseph Fourier, 41 Avenue des Martyrs, F 38027 Grenoble Cedex 1, France
Science 277:1676-81. 1997..cubic phases provide a continuous three-dimensional bilayer matrix that facilitates nucleation and growth of bacteriorhodopsin microcrystals. The crystals diffract x-rays isotropically to 2.0 angstroms... - Electron-crystallographic refinement of the structure of bacteriorhodopsinN Grigorieff
MRC Laboratory of Molecular Biology, Cambridge, UK
J Mol Biol 259:393-421. 1996..from 30 new images of tilted specimens, an improved experimental density map has been calculated for bacteriorhodopsin. The atomic model has then been rebuilt into this new map with particular attention to the surface loops... - Thr-90 plays a vital role in the structure and function of bacteriorhodopsinAlex Perálvarez Marín
Unitat de Biofisica, Departament de Bioquimica i de Biologia Molecular, Facultat de Medicina, Universitat Autonoma de Barcelona, Bellaterra Cerdanyola del Valles, Barcelona, Spain
J Biol Chem 279:16403-9. 2004The role of Thr-90 in the bacteriorhodopsin structure and function was investigated by its replacement with Ala and Val. The mutant D115A was also studied because Asp-115 in helix D forms a hydrogen bond with Thr-90 in helix C... - Analysis of high-resolution electron diffraction patterns from purple membrane labelled with heavy-atomsT A Ceska
MRC Laboratory of Molecular Biology, Cambridge, England
J Mol Biol 213:539-60. 1990Progress in the structure determination of bacteriorhodopsin, the protein component of purple membrane from Halobacterium halobium has been limited by the lack of three-dimensional phase information between 6 and 3 A resolution...
Research Grants
- STRUCTURAL STUDIES OF THE BACTERIORHODOPSIN PHOTOCYCLEHartmut Luecke; Fiscal Year: 2002DESCRIPTION (Adapted from abstract): Bacteriorhodopsin is a light-driven proton pump that has several well-characterized intermediates in its photocycle...
- LIGHT-DRIVEN ION TRANSPORT IN BACTERIAL RHODOPSINSJanos Lanyi; Fiscal Year: 2007b>Bacteriorhodopsin and halorhodopsin are the simplest ion pumps, and prototypes of the ubiquitous seven-transmembrane-helical proteins...
- Structure-Function Studies of the Proton-Gated Urea Channel from H. PyloriHartmut Luecke; Fiscal Year: 2009....
- Structure-Function Studies of Annexin I and IIHartmut Luecke; Fiscal Year: 2006..5) Co-crystallize annexin with t-PA and solve the structure of this complex after characterizing complex formation in solution. ..
- The mechanism of signal transduction in photoreceptorsHartmut Luecke; Fiscal Year: 2006..5. Determine the crystal structure of the primary Anabaena photoreceptor in the ground and signaling state. 6. Determine the structure of the complex of the Anabaena photoreceptor with its putative transducer. ..
- Structure-Function Studies of the Proton-Gated Urea Channel from H. PyloriHartmut Luecke; Fiscal Year: 2010....
- Structure-Function Studies of the Proton-Gated Urea Channel from H. PyloriHartmut Luecke; Fiscal Year: 2009....