Gene Symbol: Vha100-1
Description: Vacuolar H[+] ATPase 100kD subunit 1
Alias: BcDNA:LD21248, CG1709, Dmel\CG1709, V0a, V100, Vha100, v0a1, v100, vha, vha100-1, vacuolar H[+] ATPase 100kD subunit 1, CG1709-PA, CG1709-PB, CG1709-PC, CG1709-PD, CG1709-PE, CG1709-PF, CG1709-PG, CG1709-PI, CG1709-PJ, CG1709-PK, V0-ATPase a1, Vha100-1-PA, Vha100-1-PB, Vha100-1-PC, Vha100-1-PD, Vha100-1-PE, Vha100-1-PF, Vha100-1-PG, Vha100-1-PI, Vha100-1-PJ, Vha100-1-PK, neuron-specific V[[0]] subunit a1, vacuolar ATPase V0, subunit a1, vacuolar H[+] ATPase subunit 100-1
Species: fruit fly

Top Publications

  1. Hiesinger P, Fayyazuddin A, Mehta S, Rosenmund T, Schulze K, Zhai R, et al. The v-ATPase V0 subunit a1 is required for a late step in synaptic vesicle exocytosis in Drosophila. Cell. 2005;121:607-620 pubmed publisher
    ..We have isolated mutations in the largest V(0) component vha100-1 in flies in an unbiased genetic screen for synaptic malfunction...
  2. Zhang W, Wang D, Volk E, Bellen H, Hiesinger P, Quiocho F. V-ATPase V0 sector subunit a1 in neurons is a target of calmodulin. J Biol Chem. 2008;283:294-300 pubmed
    ..characterization of a calmodulin-binding site in the large cytosolic N-terminal region of the Drosophila protein V100, the neuron-specific V(0) subunit a1. V100 forms a tight complex with calmodulin in a Ca(2+)-dependent manner...
  3. Williamson W, Wang D, Haberman A, Hiesinger P. A dual function of V0-ATPase a1 provides an endolysosomal degradation mechanism in Drosophila melanogaster photoreceptors. J Cell Biol. 2010;189:885-99 pubmed publisher
    ..In this study, we present a dual function for the neuron-specific V0 subunit a1 orthologue v100 in Drosophila melanogaster...
  4. Williamson W, Yang T, Terman J, Hiesinger P. Guidance receptor degradation is required for neuronal connectivity in the Drosophila nervous system. PLoS Biol. 2010;8:e1000553 pubmed publisher
    ..Our findings indicate that a neuron-specific endolysosomal degradation mechanism is part of the cell biological machinery that regulates guidance receptor turnover and signaling. ..
  5. Williamson W, Hiesinger P. On the role of v-ATPase V0a1-dependent degradation in Alzheimer disease. Commun Integr Biol. 2010;3:604-7 pubmed publisher
    ..Vesicular ATPases are intracellular proton pumps that acidify autophagosomes and lysosomes. V0a1 is a key component of the v-ATPase that is only required in neurons in Drosophila melanogaster...
  6. Lee Y, Reinhardt J. Widespread polymorphism in the positions of stop codons in Drosophila melanogaster. Genome Biol Evol. 2012;4:533-49 pubmed publisher
    ..melanogaster and Drosophila simulans, and high-frequency alleles that cause extreme changes in gene length. SCPs likely have an important role in the evolution of these genes. ..
  7. Dubos A, Castells Nobau A, Meziane H, Oortveld M, Houbaert X, Iacono G, et al. Conditional depletion of intellectual disability and Parkinsonism candidate gene ATP6AP2 in fly and mouse induces cognitive impairment and neurodegeneration. Hum Mol Genet. 2015;24:6736-55 pubmed publisher
    ..Our results identify ATP6AP2 as an essential gene for the nervous system. ..
  8. Zoncu R, Bar Peled L, Efeyan A, Wang S, Sancak Y, Sabatini D. mTORC1 senses lysosomal amino acids through an inside-out mechanism that requires the vacuolar H(+)-ATPase. Science. 2011;334:678-83 pubmed publisher
    ..These results identify the v-ATPase as a component of the mTOR pathway and delineate a lysosome-associated machinery for amino acid sensing. ..
  9. Dow J. The multifunctional Drosophila melanogaster V-ATPase is encoded by a multigene family. J Bioenerg Biomembr. 1999;31:75-83 pubmed
    ..resources have accelerated our knowledge of the V-ATPase gene family size and the new Drosophila genes vhaSFD, vha100-1, vha100-2, vha100-3, vha16-2, vha16-3, vha16-4, vhaPPA1, vhaPPA2, vhaM9.7.1, and vhaM9.7.2 are described...

More Information


  1. Romero E, Cha G, Verstreken P, Ly C, Hughes R, Bellen H, et al. Suppression of neurodegeneration and increased neurotransmission caused by expanded full-length huntingtin accumulating in the cytoplasm. Neuron. 2008;57:27-40 pubmed publisher
    ..Thus, our data indicate that increased neurotransmission is at the root of neuronal degeneration caused by expanded full-length htt during early stages of pathogenesis. ..
  2. Chang Y, Tang H, Liang S, Pu T, Meng T, Khoo K, et al. Evaluation of Drosophila metabolic labeling strategies for in vivo quantitative proteomic analyses with applications to early pupa formation and amino acid starvation. J Proteome Res. 2013;12:2138-50 pubmed publisher
  3. Wang D, Epstein D, Khalaf O, Srinivasan S, Williamson W, Fayyazuddin A, et al. Ca2+-Calmodulin regulates SNARE assembly and spontaneous neurotransmitter release via v-ATPase subunit V0a1. J Cell Biol. 2014;205:21-31 pubmed publisher
    ..We show that the neuronal vacuolar-type H(+)-adenosine triphosphatase V0 subunit a1 (V100) can regulate the formation of SNARE complexes in a Ca(2+)-Calmodulin (CaM)-dependent manner...
  4. Francis D, Ghabrial A. Compensatory branching morphogenesis of stalk cells in the Drosophila trachea. Development. 2015;142:2048-57 pubmed publisher
    ..This compensatory branching also occurs in response to injury, with damaged terminal cells being rapidly invaded by their stalk cell neighbor. ..
  5. Bajjalieh S. A new view of an old pore. Cell. 2005;121:496-497 pubmed publisher
    ..In this issue of Cell, Hiesinger and coworkers (Hiesinger et al., 2005) present a forward genetic screen that reveals it to be necessary for synaptic vesicle fusion, independent of its role in vesicle acidification. ..
  6. Swetha M, Sriram V, Krishnan K, Oorschot V, Ten Brink C, Klumperman J, et al. Lysosomal membrane protein composition, acidic pH and sterol content are regulated via a light-dependent pathway in metazoan cells. Traffic. 2011;12:1037-55 pubmed publisher
    ..Depletion of the mammalian counterpart of Light, hVps41, in a human cell line also inhibits delivery of hLAMP to endosomes, suggesting an evolutionarily conserved pathway in metazoa. ..
  7. Haberman A, Williamson W, Epstein D, Wang D, Rina S, Meinertzhagen I, et al. The synaptic vesicle SNARE neuronal Synaptobrevin promotes endolysosomal degradation and prevents neurodegeneration. J Cell Biol. 2012;196:261-76 pubmed publisher
    ..Cathepsin proteases and the neuron-specific proton pump and V0 adenosine triphosphatase component V100. Overexpressing V100 partially rescued n-syb-dependent degeneration through an acidification-independent endosomal ..
  8. Coen K, Flannagan R, Baron S, Carraro Lacroix L, Wang D, Vermeire W, et al. Lysosomal calcium homeostasis defects, not proton pump defects, cause endo-lysosomal dysfunction in PSEN-deficient cells. J Cell Biol. 2012;198:23-35 pubmed publisher
    ..doi.org/10.1016/j.cell.2010.05.008) attributed this phenomenon to PSEN1 enabling the transport of mature V0a1 subunits of the vacuolar ATPase (V-ATPase) to lysosomes...
  9. Petzoldt A, Gleixner E, Fumagalli A, Vaccari T, Simons M. Elevated expression of the V-ATPase C subunit triggers JNK-dependent cell invasion and overgrowth in a Drosophila epithelium. Dis Model Mech. 2013;6:689-700 pubmed publisher
    ..In summary, our in vivo-findings demonstrate that increased levels of V-ATPase C subunit induce a Eiger/JNK-dependent cell transformation within an epithelial organ that recapitulates early carcinoma stages. ..
  10. Tian X, Gala U, Zhang Y, Shang W, Nagarkar Jaiswal S, di Ronza A, et al. A voltage-gated calcium channel regulates lysosomal fusion with endosomes and autophagosomes and is required for neuronal homeostasis. PLoS Biol. 2015;13:e1002103 pubmed publisher
    ..In summary, we present a model in which the VGCC plays a role in autophagy by regulating the fusion of AVs with lysosomes through its calcium channel activity and hence functions in maintaining neuronal homeostasis. ..