Gene Symbol: Usp8
Description: Ubiquitin specific protease 8
Alias: CG5798, CT18196, Dmel\CG5798, Q9VDD8, UBPY, UBPY/USP8, USP8, UbpY, Ubpy, dUBPY, ubpy, usp8, ubiquitin specific protease 8, CG5798-PA, CG5798-PB, UBPY ortholog, Usp8-PA, Usp8-PB
Species: fruit fly

Top Publications

  1. Chen X, Fischer J. In vivo Structure/Function analysis of the Drosophila fat facets deubiquitinating enzyme gene. Genetics. 2000;156:1829-36 pubmed
    ..The results indicate functions for specific conserved amino acids in the catalytic region of Fat facets and also indicate that regions of the protein both N- and C-terminal to the catalytic region are required for Fat facets function. ..
  2. Mukai A, Yamamoto Hino M, Awano W, Watanabe W, Komada M, Goto S. Balanced ubiquitylation and deubiquitylation of Frizzled regulate cellular responsiveness to Wg/Wnt. EMBO J. 2010;29:2114-25 pubmed publisher
    ..cells and in Drosophila, and that the cell surface level of Frizzled is regulated by deubiquitylating enzyme UBPY/ubiquitin-specific protease 8 (USP8)...
  3. Chen Y, Jiang J. Decoding the phosphorylation code in Hedgehog signal transduction. Cell Res. 2013;23:186-200 pubmed publisher
    ..In this review, we focus on the multifaceted roles that phosphorylation plays in Hh signal transduction, and discuss the conservation and difference between Drosophila and mammalian Hh signaling mechanisms. ..
  4. Xia R, Jia H, Fan J, Liu Y, Jia J. USP8 promotes smoothened signaling by preventing its ubiquitination and changing its subcellular localization. PLoS Biol. 2012;10:e1001238 pubmed publisher
    ..Using an in vivo RNAi screen, we identified ubiquitin-specific protease 8 (USP8) as a deubiquitinase that down-regulates Smo ubiquitination...
  5. Li S, Chen Y, Shi Q, Yue T, Wang B, Jiang J. Hedgehog-regulated ubiquitination controls smoothened trafficking and cell surface expression in Drosophila. PLoS Biol. 2012;10:e1001239 pubmed publisher
    ..Finally, we show that Smo ubiquitination is counteracted by the deubiquitinating enzyme UBPY/USP8. Gain and loss of UBPY lead to reciprocal changes in Smo cell surface expression...
  6. Fan J, Jiang K, Liu Y, Jia J. Hrs promotes ubiquitination and mediates endosomal trafficking of smoothened in Drosophila hedgehog signaling. PLoS ONE. 2013;8:e79021 pubmed publisher
    ..Taken together, our data indicate that Hrs mediates Smo trafficking in the late endosome by not only promoting Smo ubiquitination but also blocking Smo phosphorylation. ..
  7. Lin Y, Han M, Shimada B, Wang L, Gibler T, Amarakone A, et al. Influence of the period-dependent circadian clock on diurnal, circadian, and aperiodic gene expression in Drosophila melanogaster. Proc Natl Acad Sci U S A. 2002;99:9562-7 pubmed
    ..Thus, the period-dependent circadian clock regulates only a limited set of rhythmically expressed transcripts. Unexpectedly, period regulates basal and light-regulated gene expression to a very broad extent. ..
  8. Jacomin A, Bescond A, Soleilhac E, Gallet B, Schoehn G, Fauvarque M, et al. The Deubiquitinating Enzyme UBPY Is Required for Lysosomal Biogenesis and Productive Autophagy in Drosophila. PLoS ONE. 2015;10:e0143078 pubmed publisher
    ..This screen identified Uch-L3, Usp45, Usp12 and Ubpy. In this paper, we show that Ubpy loss of function results in the accumulation of autophagosomes due to a blockade ..
  9. Pradhan Sundd T, Verheyen E. The Myopic-Ubpy-Hrs nexus enables endosomal recycling of Frizzled. Mol Biol Cell. 2015;26:3329-42 pubmed publisher
    ..Overexpression of Hrs rescues the trafficking defect caused by mop knockdown. Mop aids in the maintenance of Ubpy, which deubiquitinates (and thus stabilizes) Hrs. In the absence of the ubiquitin ligase Cbl, Mop is dispensable...

More Information


  1. Luo W, Li Y, Tang C, Abruzzi K, Rodriguez J, Pescatore S, et al. CLOCK deubiquitylation by USP8 inhibits CLK/CYC transcription in Drosophila. Genes Dev. 2012;26:2536-49 pubmed publisher
    ..We describe here the role of ubiquitin-specific protease 8 (USP8) in circadian transcriptional repression as well as the importance of CLK ubiquitylation in CLK/CYC transcription ..
  2. Zhang W, Zou S, Song J. Term-tissue specific models for prediction of gene ontology biological processes using transcriptional profiles of aging in drosophila melanogaster. BMC Bioinformatics. 2008;9:129 pubmed publisher
    ..p < 0.01). This finding may be important in biology because the genes of mitochondria play a critical role in the longevity of C. elegans and D. melanogaster. ..
  3. Kula Eversole E, Nagoshi E, Shang Y, Rodriguez J, Allada R, Rosbash M. Surprising gene expression patterns within and between PDF-containing circadian neurons in Drosophila. Proc Natl Acad Sci U S A. 2010;107:13497-502 pubmed publisher
    ..The results suggest that RNA cycling is much more prominent in circadian neurons than elsewhere in heads and may be particularly important for the functioning of these neurons. ..
  4. Hans F, Fiesel F, Strong J, J ckel S, Rasse T, Geisler S, et al. UBE2E ubiquitin-conjugating enzymes and ubiquitin isopeptidase Y regulate TDP-43 protein ubiquitination. J Biol Chem. 2014;289:19164-79 pubmed publisher
    ..TDP-43 is ubiquitinated, we focused on the ubiquitin-conjugating enzyme UBE2E3 and the ubiquitin isopeptidase Y (UBPY). When cells were treated with proteasome inhibitor, ubiquitinated and insoluble TDP-43 species accumulated...
  5. Loncle N, Agromayor M, Martin Serrano J, Williams D. An ESCRT module is required for neuron pruning. Sci Rep. 2015;5:8461 pubmed publisher
    ..Furthermore we show that this ESCRT module requires a direct, in vivo, interaction between Shrub/CHMP4B and the accessory protein Myopic/HD-PTP. ..
  6. Ristić G, Tsou W, Guzi E, Kanack A, SCAGLIONE K, Todi S. USP5 Is Dispensable for Monoubiquitin Maintenance in Drosophila. J Biol Chem. 2016;291:9161-72 pubmed publisher
    ..Our work indicates that a primary physiological role of USP5 is not to recycle monoubiquitin for reutilization, but that it may involve disassembly of conjugated ubiquitin to maintain proteasome function. ..
  7. Yang C, Sinenko S, Thomenius M, Robeson A, Freel C, Horn S, et al. The deubiquitinating enzyme DUBAI stabilizes DIAP1 to suppress Drosophila apoptosis. Cell Death Differ. 2014;21:604-11 pubmed publisher
    ..DUBAI, therefore, represents a novel locus of apoptotic regulation in Drosophila, antagonizing cell death signals that would otherwise result in DIAP1 degradation. ..
  8. Wijnen H, Naef F, Young M. Molecular and statistical tools for circadian transcript profiling. Methods Enzymol. 2005;393:341-65 pubmed
    ..Both comparative and temporal study designs are discussed, and their use in circadian research is illustrated with examples. Data analysis methods to assess periodic components in time series data are outlined in detail. ..
  9. Yang X, Mao F, Lv X, Zhang Z, Fu L, Lu Y, et al. Drosophila Vps36 regulates Smo trafficking in Hedgehog signaling. J Cell Sci. 2013;126:4230-8 pubmed publisher
  10. Zhang J, Du J, Lei C, Liu M, Zhu A. Ubpy controls the stability of the ESCRT-0 subunit Hrs in development. Development. 2014;141:1473-9 pubmed publisher
    ..Here, we show that Ubiquitin isopeptidase Y (Ubpy) plays a conserved role in vivo in the homeostasis of an essential ESCRT-0 complex component Hrs...
  11. Takahashi A, Takahashi K, Ueda R, Takano Shimizu T. Natural variation of ebony gene controlling thoracic pigmentation in Drosophila melanogaster. Genetics. 2007;177:1233-7 pubmed
    ..It was found to be the difference in expression level of ebony, which codes for an enzyme in the melanin-synthesis pathway and has pleiotropic effects on vision and behavior. ..