Gene Symbol: TfIIS
Description: RNA polymerase II elongation factor
Alias: BG:DS00929.12, CG3710, DmS-II, DmSII, Dmel\CG3710, RnpSII, TFIIS, TFIISA, TFS-II, br52, l(2)35Cf, l(2)35cF, l(2)br52, l35Cf, RNA polymerase II elongation factor, CG3710-PA, CG3710-PB, TfIIS-PA, TfIIS-PB, lethal(2)35Cf, transcription cleavage factor IIS
Species: fruit fly

Top Publications

  1. Furukawa T, Maruyama S, Kawaichi M, Honjo T. The Drosophila homolog of the immunoglobulin recombination signal-binding protein regulates peripheral nervous system development. Cell. 1992;69:1191-7 pubmed
    ..The results also imply that the immunoglobulin recombination signal sequence and the target sequence of the Drosophila J kappa RBP protein might have a common evolutionary origin. ..
  2. Iyengar B, Roote J, Campos A. The tamas gene, identified as a mutation that disrupts larval behavior in Drosophila melanogaster, codes for the mitochondrial DNA polymerase catalytic subunit (DNApol-gamma125). Genetics. 1999;153:1809-24 pubmed
    ..Sequencing of mutant alleles demonstrated that tamas codes for the mitochondrial DNA polymerase catalytic subunit (DNApol-gamma125). ..
  3. Sluder A, Greenleaf A, Price D. Properties of a Drosophila RNA polymerase II elongation factor. J Biol Chem. 1989;264:8963-9 pubmed
    ..Kobayashi, N., Mizuno, D., and Natori, S. (1976) Biochemistry 15, 5064-5070). We have used a completely defined system and show that the properties of DmS-II are intrinsic to the factor and not mediated through other factors. ..
  4. Nechaev S, Fargo D, dos Santos G, Liu L, Gao Y, Adelman K. Global analysis of short RNAs reveals widespread promoter-proximal stalling and arrest of Pol II in Drosophila. Science. 2010;327:335-8 pubmed publisher
    ..These results indicate that the intrinsic efficiency of early elongation can greatly affect gene expression. ..
  5. Ni Z, Saunders A, Fuda N, Yao J, Suarez J, Webb W, et al. P-TEFb is critical for the maturation of RNA polymerase II into productive elongation in vivo. Mol Cell Biol. 2008;28:1161-70 pubmed
    ..Strikingly, all transcription factors tested (P-TEFb, Spt5, Spt6, and TFIIS) and RNA-processing factor CstF50 exited the body of the gene with kinetics indistinguishable from that of Pol II...
  6. Oh Y, Lee S, Yoon J, Han K, Baek K. Promoter analysis of the Drosophila melanogaster gene encoding transcription elongation factor TFIIS. Biochim Biophys Acta. 2001;1518:276-81 pubmed
    The promoter region of the Drosophila melanogaster TFIIS gene was characterized by transient expression assay...
  7. Cai Y, Chia W, Yang X. A family of snail-related zinc finger proteins regulates two distinct and parallel mechanisms that mediate Drosophila neuroblast asymmetric divisions. EMBO J. 2001;20:1704-14 pubmed
  8. Tinker R, Silver D, Montell D. Requirement for the vasa RNA helicase in gurken mRNA localization. Dev Biol. 1998;199:1-10 pubmed
  9. Chen Y, Chafin D, Price D, Greenleaf A. Drosophila RNA polymerase II mutants that affect transcription elongation. J Biol Chem. 1996;271:5993-9 pubmed
    ..Our results suggest that the S1 mutation increases the time spent by the polymerase in elongation competent mode and that the C4 mutation may affect the movement of the polymerase. ..

More Information


  1. Kephart D, Marshall N, Price D. Stability of Drosophila RNA polymerase II elongation complexes in vitro. Mol Cell Biol. 1992;12:2067-77 pubmed
  2. Oh Y, Yoon J, Baek K. Isolation and characterization of the gene encoding the Drosophila melanogaster transcriptional elongation factor, TFIIS. Biochim Biophys Acta. 1995;1262:99-103 pubmed
    We have characterized a genomic clone encoding the Drosophila melanogaster transcriptional elongation factor, TFIIS. The coding region of the TFIIS gene is interrupted by a short intron...
  3. Guo H, Price D. Mechanism of DmS-II-mediated pause suppression by Drosophila RNA polymerase II. J Biol Chem. 1993;268:18762-70 pubmed
    ..Elongation by the polymerase then allows a second encounter with the pause site and a second chance of passing the site. Complete pause suppression may require multiple transcript shortening events for some polymerase molecules. ..
  4. Kiehart D, Franke J, Chee M, Montague R, Chen T, Roote J, et al. Drosophila crinkled, mutations of which disrupt morphogenesis and cause lethality, encodes fly myosin VIIA. Genetics. 2004;168:1337-52 pubmed publisher
    ..Together, our data suggest a key role for ck/myoVIIA in the formation of cellular projections and other actin-based functions required for viability...
  5. Adelman K, Marr M, Werner J, Saunders A, Ni Z, Andrulis E, et al. Efficient release from promoter-proximal stall sites requires transcript cleavage factor TFIIS. Mol Cell. 2005;17:103-12 pubmed
    ..transcription arrest within the promoter-proximal region of Drosophila hsp70 and that transcript cleavage factor TFIIS is essential for rapid induction of hsp70 RNA...
  6. Salamov A, Solovyev V. Ab initio gene finding in Drosophila genomic DNA. Genome Res. 2000;10:516-22 pubmed
    ..The suite of gene identification programs is available through the WWW server of Computational Genomics Group at html. ..
  7. Jin J, Baek S, Lee H, Oh M, Koo Y, Shim M, et al. A DNA replication-related element downstream from the initiation site of Drosophila selenophosphate synthetase 2 gene is essential for its transcription. Nucleic Acids Res. 2004;32:2482-93 pubmed
    ..These results strongly suggest that the DRE/DREF system controls the basal level of transcription of dsps2 by interacting with TRF2. ..
  8. de Wit E, Greil F, van Steensel B. Genome-wide HP1 binding in Drosophila: developmental plasticity and genomic targeting signals. Genome Res. 2005;15:1265-73 pubmed
    ..These results provide insights into the mechanisms of HP1 targeting in the natural genomic context. ..
  9. Chafin D, Guo H, Price D. Action of alpha-amanitin during pyrophosphorolysis and elongation by RNA polymerase II. J Biol Chem. 1995;270:19114-9 pubmed
    ..Since polymerase molecules encounter similar kinetic barriers during both processes, it is possible that there is a common step in the reverse movement of the polymerase. ..
  10. Marshall T, Guo H, Price D. Drosophila RNA polymerase II elongation factor DmS-II has homology to mouse S-II and sequence similarity to yeast PPR2. Nucleic Acids Res. 1990;18:6293-8 pubmed
    DmSII is a Drosophila RNA polymerase II elongation factor which suppresses pausing by RNA polymerase II at specific sites on double stranded templates...