Gene Symbol: TfIIFbeta
Description: Transcription factor TFIIFbeta
Alias: CG6538, Dmel\CG6538, RAP30, T2FB_DROME, TFIIF, TFIIFbeta, TfIIF, dTFIIF30, l(3)j3C1, transcription factor TFIIFbeta, CG6538-PA, TFIIF small subunit, TfIIFbeta-PA, factor 5
Species: fruit fly

Top Publications

  1. Wampler S, Tyree C, Kadonaga J. Fractionation of the general RNA polymerase II transcription factors from Drosophila embryos. J Biol Chem. 1990;265:21223-31 pubmed
    ..This fractionated system should serve as a source of general transcription factors for the study of RNA polymerase II transcription in Drosophila as well as other eukaryotes. ..
  2. Yang M, Nelson D, Funakoshi Y, Padgett R. Genome-wide microarray analysis of TGFbeta signaling in the Drosophila brain. BMC Dev Biol. 2004;4:14 pubmed
    ..Many of these genes are novel and several genes are implicated in growth control. Among the genes regulated by both pathways is ultraspiracle, which further connects TGFbeta with neuronal remodeling. ..
  3. Gong D, Mortin M, Horikoshi M, Nakatani Y. Molecular cloning of cDNA encoding the small subunit of Drosophila transcription initiation factor TFIIF. Nucleic Acids Res. 1995;23:1882-6 pubmed
    Transcription initiation factor TFIIF is a tetramer consisting of two large subunits (TFIIF alpha or RAP74) and two small subunits (TFIIF beta or RAP30). We report here the molecular cloning of a Drosophila cDNA encoding TFIIF beta...
  4. Sandaltzopoulos R, Becker P. Heat shock factor increases the reinitiation rate from potentiated chromatin templates. Mol Cell Biol. 1998;18:361-7 pubmed
    ..Activator-dependent reinitiation of transcription, obviating the slow assembly of the TFIID-TFIIA complex on a promoter, may be especially crucial for genes requiring a fast response to inducers. ..
  5. Sauer F, Hansen S, Tjian R. DNA template and activator-coactivator requirements for transcriptional synergism by Drosophila bicoid. Science. 1995;270:1825-8 pubmed
    ..Thus, contact between multiple activation domains of BCD and different targets within the TFIID complex can mediate transcriptional synergism. ..
  6. Breiling A, Turner B, Bianchi M, Orlando V. General transcription factors bind promoters repressed by Polycomb group proteins. Nature. 2001;412:651-5 pubmed
    ..X-ChIP) to map TATA-binding protein (TBP), transcription initiation factor IIB (TFIIB) and IIF (TFIIF), and dHDAC1 (RPD3) across several Drosophila promoter regions...
  7. Sauer F, Hansen S, Tjian R. Multiple TAFIIs directing synergistic activation of transcription. Science. 1995;270:1783-8 pubmed
    ..Thus, the concerted action of multiple regulators with different coactivators helps to establish the pattern and level of segmentation gene transcription during Drosophila development. ..
  8. Frank D, Tyree C, George C, Kadonaga J. Structure and function of the small subunit of TFIIF (RAP30) from Drosophila melanogaster. J Biol Chem. 1995;270:6292-7 pubmed
    ..The protein encoded by the cDNA, termed dTFIIF30, was synthesized in Escherichia coli and purified to greater than 95% homogeneity...
  9. Lebedeva L, Nabirochkina E, Kurshakova M, Robert F, Krasnov A, Evgen ev M, et al. Occupancy of the Drosophila hsp70 promoter by a subset of basal transcription factors diminishes upon transcriptional activation. Proc Natl Acad Sci U S A. 2005;102:18087-92 pubmed
    ..hsp70 promoter is already occupied by TATA-binding protein (TBP) and several TBP-associated factors (TAFs), TFIIB, TFIIF (RAP30), TFIIH (XPB), TBP-free/TAF-containg complex (GCN5 and TRRAP), and the Mediator complex subunit 13 before ..

More Information


  1. Dynan W, Gilman M. Transcriptional control and signal transduction: of soap-operas and reductionism. Trends Genet. 1993;9:154-6 pubmed
  2. Kephart D, Wang B, Burton Z, Price D. Functional analysis of Drosophila factor 5 (TFIIF), a general transcription factor. J Biol Chem. 1994;269:13536-43 pubmed
    b>Factor 5 is a Drosophila RNA polymerase II initiation factor that also affects the elongation phase of transcription. We have used a cDNA encoding the large subunit of factor 5 (F5a) to produce recombinant F5a (rF5a)...
  3. Kephart D, Marshall N, Price D. Stability of Drosophila RNA polymerase II elongation complexes in vitro. Mol Cell Biol. 1992;12:2067-77 pubmed
    ..Consistent with the first effect, we find that the ability of factor 5 (TFIIF) to stimulate the elongation rate is inhibited by the disruptive agents when assayed in a defined system ..
  4. TenHarmsel A, Biggin M. Bending DNA can repress a eukaryotic basal promoter and inhibit TFIID binding. Mol Cell Biol. 1995;15:5492-8 pubmed
    ..We suggest that bending DNA may be a mechanism by which eukaryotic transcription may be regulated, by modulating the activity of the general transcription factors. ..
  5. Austin R, Biggin M. Purification of the Drosophila RNA polymerase II general transcription factors. Proc Natl Acad Sci U S A. 1996;93:5788-92 pubmed
    ..Drosophila TFIIE, TFIIF, TFIIH, and RNA polymerase II have been purified to greater than 50% homogeneity from Drosophila embryo nuclear ..
  6. Li C, Manley J. Even-skipped represses transcription by binding TATA binding protein and blocking the TFIID-TATA box interaction. Mol Cell Biol. 1998;18:3771-81 pubmed
    ..We conclude that Eve represses transcription via a direct interaction with TBP that blocks TFIID binding to the promoter. ..
  7. Li C, Manley J. Allosteric regulation of even-skipped repression activity by phosphorylation. Mol Cell. 1999;3:77-86 pubmed
    ..Our results suggest a novel mechanism of transcriptional control involving phosphorylation-induced allosteric interference with a repressive protein-protein interaction. ..
  8. Schock F, Sauer F, Jackle H, Purnell B. Drosophila head segmentation factor buttonhead interacts with the same TATA box-binding protein-associated factors and in vivo DNA targets as human Sp1 but executes a different biological program. Proc Natl Acad Sci U S A. 1999;96:5061-5 pubmed
  9. Pham A, Muller S, Sauer F. Mesoderm-determining transcription in Drosophila is alleviated by mutations in TAF(II)60 and TAF(II)110. Mech Dev. 1999;84:3-16 pubmed
    ..The results provide evidence that TAF(II)-subunits within the TFIID complex play an important role during the molecular events leading to initiation of mesoderm formation in Drosophila. ..
  10. Hansen S, Tjian R. TAFs and TFIIA mediate differential utilization of the tandem Adh promoters. Cell. 1995;82:565-75 pubmed
    ..We propose a mechanism for regulating differential promoter utilization during Drosophila development that involves the recognition of specific initiator elements by TAFs in the TFIID complex. ..