Gene Symbol: TfIIB
Description: Transcription factor IIB
Alias: CG5193, Dmel\CG5193, TFIIB, Tfiib, TfllB, dTFIIB, dTfIIB, dmTFIIB, tfIIb, transcription factor IIB, CG5193-PA, CG5193-PB, TfIIB-PA, TfIIB-PB
Species: fruit fly

Top Publications

  1. Lebedeva L, Nabirochkina E, Kurshakova M, Robert F, Krasnov A, Evgen ev M, et al. Occupancy of the Drosophila hsp70 promoter by a subset of basal transcription factors diminishes upon transcriptional activation. Proc Natl Acad Sci U S A. 2005;102:18087-92 pubmed
    ..the hsp70 promoter is already occupied by TATA-binding protein (TBP) and several TBP-associated factors (TAFs), TFIIB, TFIIF (RAP30), TFIIH (XPB), TBP-free/TAF-containg complex (GCN5 and TRRAP), and the Mediator complex subunit 13 ..
  2. Fu D, Wen Y, Ma J. The co-activator CREB-binding protein participates in enhancer-dependent activities of bicoid. J Biol Chem. 2004;279:48725-33 pubmed
    ..Together, these experiments suggest that dCBP is an enhancer-dependent co-activator of Bcd, facilitating its activation through multiple mechanisms. ..
  3. Sauer F, Hansen S, Tjian R. DNA template and activator-coactivator requirements for transcriptional synergism by Drosophila bicoid. Science. 1995;270:1825-8 pubmed
    ..Thus, contact between multiple activation domains of BCD and different targets within the TFIID complex can mediate transcriptional synergism. ..
  4. Sauer F, Hansen S, Tjian R. Multiple TAFIIs directing synergistic activation of transcription. Science. 1995;270:1783-8 pubmed
    ..Thus, the concerted action of multiple regulators with different coactivators helps to establish the pattern and level of segmentation gene transcription during Drosophila development. ..
  5. Wampler S, Tyree C, Kadonaga J. Fractionation of the general RNA polymerase II transcription factors from Drosophila embryos. J Biol Chem. 1990;265:21223-31 pubmed
    ..This fractionated system should serve as a source of general transcription factors for the study of RNA polymerase II transcription in Drosophila as well as other eukaryotes. ..
  6. Hansen S, Tjian R. TAFs and TFIIA mediate differential utilization of the tandem Adh promoters. Cell. 1995;82:565-75 pubmed
    ..We propose a mechanism for regulating differential promoter utilization during Drosophila development that involves the recognition of specific initiator elements by TAFs in the TFIID complex. ..
  7. TenHarmsel A, Biggin M. Bending DNA can repress a eukaryotic basal promoter and inhibit TFIID binding. Mol Cell Biol. 1995;15:5492-8 pubmed
    ..We suggest that bending DNA may be a mechanism by which eukaryotic transcription may be regulated, by modulating the activity of the general transcription factors. ..
  8. Gupta R, Emili A, Pan G, Xiao H, Shales M, Greenblatt J, et al. Characterization of the interaction between the acidic activation domain of VP16 and the RNA polymerase II initiation factor TFIIB. Nucleic Acids Res. 1996;24:2324-30 pubmed
    ..suggested for direct contact by herpes simplex virus virion protein VP16, including the general initiation factor TFIIB. In this report we have used several strategies to critically assess this interaction between VP16 and TFIIB...
  9. Lira DeVito L, Burke T, Kadonaga J. Structure of the genes encoding transcription factor IIB and TATA box-binding protein from Drosophila melanogaster. Gene. 1995;153:203-7 pubmed
    ..of the basal RNA polymerase II transcriptional machinery, the Drosophila melanogaster genes encoding transcription factor IIB (TFIIB) and the TATA-box-binding protein (TBP) were isolated and characterized...

More Information


  1. Pham A, Muller S, Sauer F. Mesoderm-determining transcription in Drosophila is alleviated by mutations in TAF(II)60 and TAF(II)110. Mech Dev. 1999;84:3-16 pubmed
    ..The results provide evidence that TAF(II)-subunits within the TFIID complex play an important role during the molecular events leading to initiation of mesoderm formation in Drosophila. ..
  2. Sandaltzopoulos R, Becker P. Heat shock factor increases the reinitiation rate from potentiated chromatin templates. Mol Cell Biol. 1998;18:361-7 pubmed
    ..Well-documented transcription activation mechanisms, such as the recruitment of TFIID and TFIIB, control the early phases of preinitiation complex formation...
  3. Colgan J, Wampler S, Manley J. Interaction between a transcriptional activator and transcription factor IIB in vivo. Nature. 1993;362:549-53 pubmed
    ..possibly with the cooperation of TFIIA, binds to the TATA element of the promoter, forming a complex that can bind TFIIB (refs 6, 7) followed by RNA polymerase II (refs 6, 8) and other factors...
  4. Manley J, Um M, Li C, Ashali H. Mechanisms of transcriptional activation and repression can both involve TFIID. Philos Trans R Soc Lond B Biol Sci. 1996;351:517-26 pubmed
    ..These results strengthen the view that TBP, either directly or indirectly as a component of TFIID, can be a target of both activators and repressors. ..
  5. Tiwari P, Kumar A, Das R, Malhotra V, Vijayraghavan K. A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction. PLoS ONE. 2015;10:e0140976 pubmed publisher
    ..Tango1 knockdown was found to affect development of muscle attachment sites and formation of myotendinous junction. Tango1 was also found to be involved in secretion of Viking (Collagen type IV) and BM-40 from hemocytes and fat cells. ..
  6. Wang Y, Duttke S, Chen K, Johnston J, Kassavetis G, Zeitlinger J, et al. TRF2, but not TBP, mediates the transcription of ribosomal protein genes. Genes Dev. 2014;28:1550-5 pubmed publisher
    ..Hence, a specialized TRF2-based RNA polymerase II system functions in the synthesis of RPs and complements the RNA polymerase I and III systems. ..
  7. Santoso B, Kadonaga J. Reconstitution of chromatin transcription with purified components reveals a chromatin-specific repressive activity of p300. Nat Struct Mol Biol. 2006;13:131-9 pubmed
    ..Hence, the mechanism of transcriptional repression by p300 is distinct from that of histone H1, PARP-1 or Sir2. These findings reveal a novel chromatin-specific repressive function of p300. ..
  8. Joshi C, Nguyen H. 5' untranslated leader sequences of eukaryotic mRNAs encoding heat shock induced proteins. Nucleic Acids Res. 1995;23:541-9 pubmed
    ..The majority of the HSP mRNA sequences (77%) contained one or more small sequence motifs suggesting that they may function as internal ribosome entry sites for selective initiation of translation during heat stress. ..
  9. Colgan J, Ashali H, Manley J. A direct interaction between a glutamine-rich activator and the N terminus of TFIIB can mediate transcriptional activation in vivo. Mol Cell Biol. 1995;15:2311-20 pubmed
    ..examining the mechanism by which transcriptional activators function have suggested that the general transcription factor IIB (TFIIB) can be a target for certain regulatory proteins...
  10. Fu D, Ma J. Interplay between positive and negative activities that influence the role of Bicoid in transcription. Nucleic Acids Res. 2005;33:3985-93 pubmed access the hb enhancer element in cells but also the occupancy of the general transcription factors TBP and TFIIB at the reporter promoter...
  11. Mason P, Lis J. Cooperative and competitive protein interactions at the hsp70 promoter. J Biol Chem. 1997;272:33227-33 pubmed
  12. George C, Lira DeVito L, Wampler S, Kadonaga J. A spectrum of mechanisms for the assembly of the RNA polymerase II transcription preinitiation complex. Mol Cell Biol. 1995;15:1049-59 pubmed
    ..a novel biochemical approach that involves perturbation of the transcription reaction with exogenously added TFIIB or TATA box-binding protein (TBP)...
  13. Li C, Manley J. Allosteric regulation of even-skipped repression activity by phosphorylation. Mol Cell. 1999;3:77-86 pubmed
    ..Our results suggest a novel mechanism of transcriptional control involving phosphorylation-induced allosteric interference with a repressive protein-protein interaction. ..
  14. Rabenstein M, Zhou S, Lis J, Tjian R. TATA box-binding protein (TBP)-related factor 2 (TRF2), a third member of the TBP family. Proc Natl Acad Sci U S A. 1999;96:4791-6 pubmed
    ..Like TRF1 and TBP, TRF2 binds transcription factor IIA (TFIIA) and TFIIB and appears to be part of a larger protein complex...
  15. Schock F, Sauer F, Jackle H, Purnell B. Drosophila head segmentation factor buttonhead interacts with the same TATA box-binding protein-associated factors and in vivo DNA targets as human Sp1 but executes a different biological program. Proc Natl Acad Sci U S A. 1999;96:5061-5 pubmed
  16. Pazin M, Hermann J, Kadonaga J. Promoter structure and transcriptional activation with chromatin templates assembled in vitro. A single Gal4-VP16 dimer binds to chromatin or to DNA with comparable affinity. J Biol Chem. 1998;273:34653-60 pubmed
  17. Breiling A, Turner B, Bianchi M, Orlando V. General transcription factors bind promoters repressed by Polycomb group proteins. Nature. 2001;412:651-5 pubmed
    ..chromatin immunoprecipitation (X-ChIP) to map TATA-binding protein (TBP), transcription initiation factor IIB (TFIIB) and IIF (TFIIF), and dHDAC1 (RPD3) across several Drosophila promoter regions...
  18. Wampler S, Kadonaga J. Functional analysis of Drosophila transcription factor IIB. Genes Dev. 1992;6:1542-52 pubmed
    We have isolated a cDNA encoding Drosophila transcription factor IIB (dTFIIB) and characterized the properties of recombinant dTFIIB with a reconstituted in vitro transcription system derived from Drosophila embryos...
  19. Goodrich J, Hoey T, Thut C, Admon A, Tjian R. Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB. Cell. 1993;75:519-30 pubmed
    ..In addition, affinity chromatography indicated a direct binding of the basal factor TFIIB to immobilized dTAFII40...
  20. Austin R, Biggin M. A domain of the even-skipped protein represses transcription by preventing TFIID binding to a promoter: repression by cooperative blocking. Mol Cell Biol. 1995;15:4683-93 pubmed
    ..We speculate that occupancy of secondary sites within the basal promoter by CDGB or the eve protein inhibits subsequent TFIID binding to repress transcription, a mechanism we term cooperative blocking. ..
  21. Lam K, Mühlpfordt F, Vaquerizas J, Raja S, Holz H, Luscombe N, et al. The NSL complex regulates housekeeping genes in Drosophila. PLoS Genet. 2012;8:e1002736 pubmed publisher
    ..The observed Pol II reduction coincides with compromised binding of TBP and TFIIB to target promoters, indicating that the NSL complex is required for optimal recruitment of the pre-initiation ..
  22. Morris D, Lee J, Sterner D, Brickey W, Greenleaf A. Assaying CTD kinases in vitro and phosphorylation-modulated properties of RNA polymerase II in vivo. Methods. 1997;12:264-75 pubmed
    ..The methods described here should, in conjunction with genetic approaches, contribute to elucidating the physiological roles of CTD kinases. ..
  23. Dynan W, Gilman M. Transcriptional control and signal transduction: of soap-operas and reductionism. Trends Genet. 1993;9:154-6 pubmed
  24. Frank D, Tyree C, George C, Kadonaga J. Structure and function of the small subunit of TFIIF (RAP30) from Drosophila melanogaster. J Biol Chem. 1995;270:6292-7 pubmed
  25. Sauer F, Fondell J, Ohkuma Y, Roeder R, Jackle H. Control of transcription by Krüppel through interactions with TFIIB and TFIIE beta. Nature. 1995;375:162-4 pubmed
    ..12). Our results indicate that when acting from a site close to a basal promoter, monomeric Kr interacts with TFIIB to activate transcription, whereas an interaction of the Kr dimer with TFIIE beta, a subunit of TFIIE, results in ..
  26. Roberts S, Green M. Transcription. Dichotomous regulators. Nature. 1995;375:105-6 pubmed
  27. Guglielmi B, La Rochelle N, Tjian R. Gene-specific transcriptional mechanisms at the histone gene cluster revealed by single-cell imaging. Mol Cell. 2013;51:480-92 pubmed publisher
    ..Surprisingly, no TFIIB or TFIID was detectable or functionally required at the initiation complexes of these promoters...
  28. Li C, Manley J. Even-skipped represses transcription by binding TATA binding protein and blocking the TFIID-TATA box interaction. Mol Cell Biol. 1998;18:3771-81 pubmed in vitro transcription systems reconstituted with either all the general transcription factors or only TBP, TFIIB, TFIIF30, and RNA polymerase II...
  29. Lee K, Oh Y, Baek G, Yoon J, Han K, Cho N, et al. Analysis of the structure and expression fo the TFIIB gene in Drosophila melanogaster. Mol Cells. 1997;7:374-9 pubmed
    We have isolated and characterized a genomic clone encoding the Drosophila melanogaster transcription factor IIB (TFIIB). The coding region of the TFIIB gene is interrupted by three short introns...
  30. Yamashita S, Wada K, Horikoshi M, Gong D, Kokubo T, Hisatake K, et al. Isolation and characterization of a cDNA encoding Drosophila transcription factor TFIIB. Proc Natl Acad Sci U S A. 1992;89:2839-43 pubmed
    A Drosophila cDNA encoding a human transcription factor TFIIB homologue was isolated by PCR methods...