Gene Symbol: Tbp
Description: TATA binding protein
Alias: CG9874, Dmel\CG9874, RBP, TBP, TBP38, TFIID, TFIIDtau, TfIID, dTBP, dTFIID, dTbp, dtbp, TATA binding protein, 42kDa polypeptide, CG9874-PA, TATA box binding protein, TATA box-binding protein, TATA-binding protein, TATA-box binding protein, Tbp-PA
Species: fruit fly
Products:     Tbp

Top Publications

  1. Giardina C, Pérez Riba M, Lis J. Promoter melting and TFIID complexes on Drosophila genes in vivo. Genes Dev. 1992;6:2190-200 pubmed
    ..This protection appears to be a consequence of TFIID binding, as a similar protection pattern could be produced in vitro with purified protein.
  2. Sauer F, Hansen S, Tjian R. Multiple TAFIIs directing synergistic activation of transcription. Science. 1995;270:1783-8 pubmed
    ..A quadruple complex containing TATA binding protein (TBP), TAFII250, TAFII110, and TAFII60 mediated transcriptional synergism by BCD and HB, whereas triple TBP-..
  3. Rabenstein M, Zhou S, Lis J, Tjian R. TATA box-binding protein (TBP)-related factor 2 (TRF2), a third member of the TBP family. Proc Natl Acad Sci U S A. 1999;96:4791-6 pubmed
    The TATA box-binding protein (TBP) is an essential component of the RNA polymerase II transcription apparatus in eukaryotic cells...
  4. Levine M, Tjian R. Transcription regulation and animal diversity. Nature. 2003;424:147-51 pubmed
    ..How, then, has evolutionary diversity arisen? Emerging evidence suggests that organismal complexity arises from progressively more elaborate regulation of gene expression. ..
  5. Fregoso M, Laine J, Aguilar Fuentes J, Mocquet V, Reynaud E, Coin F, et al. DNA repair and transcriptional deficiencies caused by mutations in the Drosophila p52 subunit of TFIIH generate developmental defects and chromosome fragility. Mol Cell Biol. 2007;27:3640-50 pubmed
    ..This study significantly extends the role of p52 in regulating XPB ATPase activity and, consequently, both its transcriptional and nucleotide excision repair functions...
  6. Fu D, Wen Y, Ma J. The co-activator CREB-binding protein participates in enhancer-dependent activities of bicoid. J Biol Chem. 2004;279:48725-33 pubmed
    ..Together, these experiments suggest that dCBP is an enhancer-dependent co-activator of Bcd, facilitating its activation through multiple mechanisms. ..
  7. Lebedeva L, Nabirochkina E, Kurshakova M, Robert F, Krasnov A, Evgen ev M, et al. Occupancy of the Drosophila hsp70 promoter by a subset of basal transcription factors diminishes upon transcriptional activation. Proc Natl Acad Sci U S A. 2005;102:18087-92 pubmed
    ..These studies indicate that the hsp70 promoter is already occupied by TATA-binding protein (TBP) and several TBP-associated factors (TAFs), TFIIB, TFIIF (RAP30), TFIIH (XPB), TBP-free/TAF-containg complex (GCN5 ..
  8. Zhang Z, Boskovic Z, Hussain M, Hu W, Inouye C, Kim H, et al. Chemical perturbation of an intrinsically disordered region of TFIID distinguishes two modes of transcription initiation. elife. 2015;4: pubmed publisher
    ..of a tin(IV) oxochloride-derived cluster that binds an evolutionarily conserved IDR within the metazoan TFIID transcription complex...
  9. Colgan J, Wampler S, Manley J. Interaction between a transcriptional activator and transcription factor IIB in vivo. Nature. 1993;362:549-53 pubmed
    ..steps is thought to be facilitated by gene-specific transcriptional activation proteins; this seems to require TFIID-associated auxiliary factors and may involve direct contact between the activator and TFIID and/or TFIIB...

More Information


  1. Crowley T, Hoey T, Liu J, Jan Y, Jan L, Tjian R. A new factor related to TATA-binding protein has highly restricted expression patterns in Drosophila. Nature. 1993;361:557-61 pubmed
    The TATA-binding protein TBP is necessary for the transcription of eukaryotic genes...
  2. Choi T, Park S, Kang H, Cheong J, Kim H, Lee B, et al. Redox regulation of DNA binding activity of DREF (DNA replication-related element binding factor) in Drosophila. Biochem J. 2004;378:833-8 pubmed
    ..These findings give insight into the exact nature of DREF function in the regulation of target genes by the intracellular redox state. ..
  3. Jin J, Baek S, Lee H, Oh M, Koo Y, Shim M, et al. A DNA replication-related element downstream from the initiation site of Drosophila selenophosphate synthetase 2 gene is essential for its transcription. Nucleic Acids Res. 2004;32:2482-93 pubmed
    ..double-stranded RNA interference (dsRNAi) experiments were performed to ablate transcription factors such as TBP, TRF1, TRF2 and DREF in Schneider cells...
  4. George C, Lira DeVito L, Wampler S, Kadonaga J. A spectrum of mechanisms for the assembly of the RNA polymerase II transcription preinitiation complex. Mol Cell Biol. 1995;15:1049-59 pubmed
    ..that involves perturbation of the transcription reaction with exogenously added TFIIB or TATA box-binding protein (TBP). Under these conditions, we observe promoter-selective inhibition of transcription by excess TFIIB or excess TBP...
  5. Papp B, Muller J. Histone trimethylation and the maintenance of transcriptional ON and OFF states by trxG and PcG proteins. Genes Dev. 2006;20:2041-54 pubmed
  6. Wu Y, Rasmussen M, Kellis M. Evolution at the subgene level: domain rearrangements in the Drosophila phylogeny. Mol Biol Evol. 2012;29:689-705 pubmed publisher
    ..These results dramatically expand on evolution at the subgene level and offer several insights into how new genes and functions arise between species. ..
  7. Zhou J, Zwicker J, Szymanski P, Levine M, Tjian R. TAFII mutations disrupt Dorsal activation in the Drosophila embryo. Proc Natl Acad Sci U S A. 1998;95:13483-8 pubmed
    In this study, we present evidence that the Dorsal activator interacts with limiting amounts of the TFIID complex in the Drosophila embryo...
  8. Gilmour D, Dietz T, Elgin S. UV cross-linking identifies four polypeptides that require the TATA box to bind to the Drosophila hsp70 promoter. Mol Cell Biol. 1990;10:4233-8 pubmed
    ..Both the extended footprint and the polypeptides identified by UV cross-linking indicate that the Drosophila TATA factor is a multicomponent complex. ..
  9. Verrijzer C, Chen J, Yokomori K, Tjian R. Binding of TAFs to core elements directs promoter selectivity by RNA polymerase II. Cell. 1995;81:1115-25 pubmed
    ..Here, we have assessed the potential role of TAFs and the TFIID complex in directing basal promoter function...
  10. Chen X, Farmer G, Zhu H, Prywes R, Prives C. Cooperative DNA binding of p53 with TFIID (TBP): a possible mechanism for transcriptional activation. Genes Dev. 1993;7:1837-49 pubmed
    ..that p53 binds directly to the TATA box-binding protein (TBP) subunit of the basal transcription factor TFIID. Here, we show that p53 cooperates with either recombinant TBP or partially purified TFIID in binding to a DNA ..
  11. Yu Z, Teng X, Bonini N. Triplet repeat-derived siRNAs enhance RNA-mediated toxicity in a Drosophila model for myotonic dystrophy. PLoS Genet. 2011;7:e1001340 pubmed publisher
    ..These small RNAs targeted the expression of CAG-containing genes, such as Ataxin-2 and TATA binding protein (TBP), which bear long CAG repeats in both fly and man...
  12. Wu C. Two protein-binding sites in chromatin implicated in the activation of heat-shock genes. Nature. 1984;309:229-34 pubmed
    ..The pattern of resistance before and after induction of gene expression suggests that heat-shock genes are activated by the sequential binding of at least two protein factors. ..
  13. Chen J, Tjian R. Reconstitution of TATA-binding protein-associated factor/TATA-binding protein complexes for in vitro transcription. Methods Enzymol. 1996;273:208-17 pubmed
  14. Kokubo T, Gong D, Yamashita S, Horikoshi M, Roeder R, Nakatani Y. Drosophila 230-kD TFIID subunit, a functional homolog of the human cell cycle gene product, negatively regulates DNA binding of the TATA box-binding subunit of TFIID. Genes Dev. 1993;7:1033-46 pubmed
    A Drosophila cDNA encoding the largest TFIID subunit (p230) was isolated using a degenerate oligodeoxynucleotide probe based on an amino acid sequence of the purified protein...
  15. Zamrod Z, Tyree C, Song Y, Stumph W. In vitro transcription of a Drosophila U1 small nuclear RNA gene requires TATA box-binding protein and two proximal cis-acting elements with stringent spacing requirements. Mol Cell Biol. 1993;13:5918-27 pubmed
    ..Finally, evidence is presented that the TATA box-binding protein is required for transcription from the wild-type U1 promoter as well as from the TATA-containing U1 promoter. ..
  16. Pereira L, van der Knaap J, Van Den Boom V, van den Heuvel F, Timmers H. TAF(II)170 interacts with the concave surface of TATA-binding protein to inhibit its DNA binding activity. Mol Cell Biol. 2001;21:7523-34 pubmed
    The human RNA polymerase II transcription factor B-TFIID consists of TATA-binding protein (TBP) and the TBP-associated factor (TAF) TAF(II)170 and can rapidly redistribute over promoter DNA...
  17. Fu D, Ma J. Interplay between positive and negative activities that influence the role of Bicoid in transcription. Nucleic Acids Res. 2005;33:3985-93 pubmed
    ..Bcd(A57-61) to access the hb enhancer element in cells but also the occupancy of the general transcription factors TBP and TFIIB at the reporter promoter...
  18. Weber J, Gilmour D. Genomic footprinting of the hsp70 and histone H3 promoters in Drosophila embryos reveals novel protein-DNA interactions. Nucleic Acids Res. 1995;23:3327-34 pubmed
    ..It has been shown previously that the TBP subunit of TFIID is associated with the TATA element and that RNA polymerase II is paused downstream from the transcription start ..
  19. Kokubo T, Gong D, Yamashita S, Takada R, Roeder R, Horikoshi M, et al. Molecular cloning, expression, and characterization of the Drosophila 85-kilodalton TFIID subunit. Mol Cell Biol. 1993;13:7859-63 pubmed
    Transcription initiation factor TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors...
  20. Gurskiy D, Nabirochkina E, Kopytova D, Nikolenko Y, Ilyin Y, Georgieva S, et al. ENY2 protein forms a part of the THO complex of Drosophila melanogaster. Dokl Biochem Biophys. 2010;433:212-5 pubmed publisher
  21. Huang D, Chang Y, Yang C, Pan I, King B. pipsqueak encodes a factor essential for sequence-specific targeting of a polycomb group protein complex. Mol Cell Biol. 2002;22:6261-71 pubmed
    ..The functional relevance of psq to the silencing mechanism is strongly supported by its synergistic interactions with a subset of Pc-G that cause misexpression of homeotic genes. ..
  22. Gill G, Tjian R. A highly conserved domain of TFIID displays species specificity in vivo. Cell. 1991;65:333-40 pubmed
    Recombinant TFIID proteins from yeast, Drosophila, and human function interchangeably in vitro to restore basal level transcription to a human HeLa extract depleted for TFIID...
  23. Chen J, Attardi L, Verrijzer C, Yokomori K, Tjian R. Assembly of recombinant TFIID reveals differential coactivator requirements for distinct transcriptional activators. Cell. 1994;79:93-105 pubmed
    We previously reported that transcriptional regulators can bind selected TAF subunits of the TFIID complex. However, the specificity and function of individual TAFs in mediating transcriptional activation remained unknown...
  24. Li C, Manley J. Even-skipped represses transcription by binding TATA binding protein and blocking the TFIID-TATA box interaction. Mol Cell Biol. 1998;18:3771-81 pubmed
    ..describe experiments indicating that the mechanism of Eve repression involves a direct interaction with the TATA binding protein (TBP) that blocks binding of TBP-TFIID to the promoter...
  25. Wang Z, Lindquist S. Developmentally regulated nuclear transport of transcription factors in Drosophila embryos enable the heat shock response. Development. 1998;125:4841-50 pubmed
    ..first time the nuclear import patterns of two general transcription factors, RNA polymerase subunit Ilc and TATA binding protein (TBP). Both enter nuclei in a highly synchronous manner, independently of each other and of HSF...
  26. Berk A. TBP-like factors come into focus. Cell. 2000;103:5-8 pubmed
  27. Breiling A, O Neill L, D Eliseo D, Turner B, Orlando V. Epigenome changes in active and inactive polycomb-group-controlled regions. EMBO Rep. 2004;5:976-82 pubmed
    ..These PcG-specific repressive marks are maintained by the action of the E(z) HMT, an enzyme that has an important role not only in establishing but also in maintaining PcG repression. ..
  28. Lis J. Promoter-associated pausing in promoter architecture and postinitiation transcriptional regulation. Cold Spring Harb Symp Quant Biol. 1998;63:347-56 pubmed
  29. Burke T, Willy P, Kutach A, Butler J, Kadonaga J. The DPE, a conserved downstream core promoter element that is functionally analogous to the TATA box. Cold Spring Harb Symp Quant Biol. 1998;63:75-82 pubmed
  30. Burke T, Kadonaga J. Drosophila TFIID binds to a conserved downstream basal promoter element that is present in many TATA-box-deficient promoters. Genes Dev. 1996;10:711-24 pubmed
    ..is present in a subset of Drosophila TATA-box-deficient (TATA-less) promoters by using purified, epitope-tagged TFIID complex (eTFIID) from embryos of transgenic Drosophila...
  31. Lebedeva L, Georgieva S, Nabirochkina E. Study of the properties of two homologues of yeast ADA2 in Drosophila melanogaster. Dokl Biochem Biophys. 2004;398:297-9 pubmed
  32. Kang J, Kang Y, Stumph W. TFIIIB subunit locations on U6 gene promoter DNA mapped by site-specific protein-DNA photo-cross-linking. FEBS Lett. 2016;590:1488-97 pubmed publisher
    ..U6 TATA sequences are bound by TFIIIB that in Drosophila contains the three subunits TBP, Brf1, and Bdp1. The overall structure of TFIIIB is still not well understood...
  33. Gangloff Y, Pointud J, Thuault S, Carre L, Romier C, Muratoglu S, et al. The TFIID components human TAF(II)140 and Drosophila BIP2 (TAF(II)155) are novel metazoan homologues of yeast TAF(II)47 containing a histone fold and a PHD finger. Mol Cell Biol. 2001;21:5109-21 pubmed
    The RNA polymerase II transcription factor TFIID comprises the TATA binding protein (TBP) and a set of TBP-associated factors (TAF(II)s)...
  34. Garber M, Vilalta A, Johnson D. Induction of Drosophila RNA polymerase III gene expression by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) is mediated by transcription factor IIIB. Mol Cell Biol. 1994;14:339-47 pubmed
  35. Hsu T, Wang C, Yang C, Lee L, Hsieh Li H, Ro L, et al. Deactivation of TBP contributes to SCA17 pathogenesis. Hum Mol Genet. 2014;23:6878-93 pubmed publisher
    ..Dysfunctional TBP also disrupts normal TBP function. Furthermore, heterozygous dTbp amorph mutant flies exhibited SCA17-like phenotypes and flies expressing polyQ-expanded TBP exhibited enhanced ..
  36. Takada S, Lis J, Zhou S, Tjian R. A TRF1:BRF complex directs Drosophila RNA polymerase III transcription. Cell. 2000;101:459-69 pubmed
    It has been generally accepted that the TATA binding protein (TBP) is a universal mediator of transcription by RNA polymerase I, II, and III...
  37. TenHarmsel A, Biggin M. Bending DNA can repress a eukaryotic basal promoter and inhibit TFIID binding. Mol Cell Biol. 1995;15:5492-8 pubmed
    ..Rather, repression occurs, at least in part, because binding of the general transcription factor TFIID to the minicircle is strongly inhibited compared with binding to the linear DNA...
  38. Pham A, Sauer F. Ubiquitin-activating/conjugating activity of TAFII250, a mediator of activation of gene expression in Drosophila. Science. 2000;289:2357-60 pubmed
    ..identified the Drosophila coactivator TAFII250, the central subunit within the general transcription factor TFIID, as a histone-specific ubiquitin-activating/conjugating enzyme (ubac)...
  39. Hansen S, Tjian R. TAFs and TFIIA mediate differential utilization of the tandem Adh promoters. Cell. 1995;82:565-75 pubmed
    ..We propose a mechanism for regulating differential promoter utilization during Drosophila development that involves the recognition of specific initiator elements by TAFs in the TFIID complex.
  40. Albright S, Tjian R. TAFs revisited: more data reveal new twists and confirm old ideas. Gene. 2000;242:1-13 pubmed
    ..Coordinating the interaction of these proteins is the basal transcription factor TFIID, which recognizes the core promoter and supplies a scaffolding upon which the rest of the transcriptional ..
  41. Santoso B, Kadonaga J. Reconstitution of chromatin transcription with purified components reveals a chromatin-specific repressive activity of p300. Nat Struct Mol Biol. 2006;13:131-9 pubmed
    ..Hence, the mechanism of transcriptional repression by p300 is distinct from that of histone H1, PARP-1 or Sir2. These findings reveal a novel chromatin-specific repressive function of p300. ..
  42. Aguilar Fuentes J, Fregoso M, Herrera M, Reynaud E, Braun C, Egly J, et al. p8/TTDA overexpression enhances UV-irradiation resistance and suppresses TFIIH mutations in a Drosophila trichothiodystrophy model. PLoS Genet. 2008;4:e1000253 pubmed publisher
    ..This study is the first using an intact higher-animal model to show that one subunit mutant can trans-complement another subunit in a multi-subunit complex linked to human diseases. ..
  43. Kokubo T, Gong D, Wootton J, Horikoshi M, Roeder R, Nakatani Y. Molecular cloning of Drosophila TFIID subunits. Nature. 1994;367:484-7 pubmed
    Transcription initiation factor TFIID is a multisubunit complex containing a TATA-box-binding factor (TFIID tau/TBP) and associated polypeptide factors (TAFs) with sizes ranging from M(r) approximately 20,000 to > 200,000...
  44. Guglielmi B, La Rochelle N, Tjian R. Gene-specific transcriptional mechanisms at the histone gene cluster revealed by single-cell imaging. Mol Cell. 2013;51:480-92 pubmed publisher
    ..Surprisingly, no TFIIB or TFIID was detectable or functionally required at the initiation complexes of these promoters...
  45. Farmer G, Colgan J, Nakatani Y, Manley J, Prives C. Functional interaction between p53, the TATA-binding protein (TBP), andTBP-associated factors in vivo. Mol Cell Biol. 1996;16:4295-304 pubmed
    The transcriptional activator p53 is known to interact with components of the general transcription factor TFIID in vitro...
  46. Duttke S. Evolution and diversification of the basal transcription machinery. Trends Biochem Sci. 2015;40:127-9 pubmed publisher
    ..the basal transcription machinery greatly diversified during evolution and new studies now demonstrate that diversification of the TATA-binding protein (TBP) family yielded specialized and largely independent transcription systems.
  47. Weber J, Taxman D, Lu Q, Gilmour D. Molecular architecture of the hsp70 promoter after deletion of the TATA box or the upstream regulation region. Mol Cell Biol. 1997;17:3799-808 pubmed
    GAGA factor, TFIID, and paused polymerase are present on the hsp70 promoter in Drosophila melanogaster prior to transcriptional activation...
  48. Theisen J, Lim C, Kadonaga J. Three key subregions contribute to the function of the downstream RNA polymerase II core promoter. Mol Cell Biol. 2010;30:3471-9 pubmed publisher
    ..We found that the MTE promotes the binding of purified TFIID to the core promoter and that the TAF6 and TAF9 subunits of TFIID appear to be in close proximity to the MTE...
  49. Hohmura K, Shi H, Hirayoshi K. Perturbation of discrete sites on a single protein domain with RNA aptamers: targeting of different sides of the TATA-binding protein (TBP). Biosci Biotechnol Biochem. 2013;77:1739-46 pubmed
    ..RNA aptamers against a protein that constitutes a single structural domain, the Drosophila TATA-binding protein (TBP)...
  50. Lira DeVito L, Burke T, Kadonaga J. Structure of the genes encoding transcription factor IIB and TATA box-binding protein from Drosophila melanogaster. Gene. 1995;153:203-7 pubmed
    ..the Drosophila melanogaster genes encoding transcription factor IIB (TFIIB) and the TATA-box-binding protein (TBP) were isolated and characterized...
  51. Frank D, Tyree C, George C, Kadonaga J. Structure and function of the small subunit of TFIIF (RAP30) from Drosophila melanogaster. J Biol Chem. 1995;270:6292-7 pubmed
  52. Yokomori K, Admon A, Goodrich J, Chen J, Tjian R. Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex. Genes Dev. 1993;7:2235-45 pubmed
    ..While characterizing the Drosophila TFIID complex, we discovered that a 30-kD protein that cofractionated with dTFIID was homologous to the previously identified, large subunit of yeast TFIIA...
  53. Liu Q, Jindra M, Ueda H, Hiromi Y, Hirose S. Drosophila MBF1 is a co-activator for Tracheae Defective and contributes to the formation of tracheal and nervous systems. Development. 2003;130:719-28 pubmed
    ..Through immunoprecipitation, MBF1 was found to form a ternary complex including MBF1, TATA-binding protein (TBP) and the bZIP protein Tracheae Defective (TDF)/Apontic...
  54. Kokubo T, Gong D, Roeder R, Horikoshi M, Nakatani Y. The Drosophila 110-kDa transcription factor TFIID subunit directly interacts with the N-terminal region of the 230-kDa subunit. Proc Natl Acad Sci U S A. 1993;90:5896-900 pubmed
    Transcription initiation factor TFIID is a multimeric protein complex that plays a central role in transcriptional regulation by facilitating promoter responses to various activators...
  55. Kokubo T, Yamashita S, Horikoshi M, Roeder R, Nakatani Y. Interaction between the N-terminal domain of the 230-kDa subunit and the TATA box-binding subunit of TFIID negatively regulates TATA-box binding. Proc Natl Acad Sci U S A. 1994;91:3520-4 pubmed
    Transcription initiation factor TFIID plays a central role in transcriptional regulation. Drosophila TFIID is a multimeric protein consisting of the TATA box-binding polypeptide (TBP) and a number of tightly associated polypeptides...
  56. Breiling A, Turner B, Bianchi M, Orlando V. General transcription factors bind promoters repressed by Polycomb group proteins. Nature. 2001;412:651-5 pubmed
    ..address this, we used formaldehyde-crosslinked chromatin immunoprecipitation (X-ChIP) to map TATA-binding protein (TBP), transcription initiation factor IIB (TFIIB) and IIF (TFIIF), and dHDAC1 (RPD3) across several Drosophila promoter ..
  57. Burke T, Kadonaga J. The downstream core promoter element, DPE, is conserved from Drosophila to humans and is recognized by TAFII60 of Drosophila. Genes Dev. 1997;11:3020-31 pubmed
    ..a seven- to eightfold reduction in transcription as well as a significant reduction in the binding of purified TFIID. These results suggest a specific and somewhat rigid interaction of TFIID with the Inr and DPE sequences...
  58. Vorobyeva N, Soshnikova N, Kuzmina J, Kopantseva M, Nikolenko J, Nabirochkina E, et al. The novel regulator of metazoan development SAYP organizes a nuclear coactivator supercomplex. Cell Cycle. 2009;8:2152-6 pubmed
    ..is related to the conserved domain SAY, which assembles a nuclear supercomplex BTFly consisting of Brahma and TFIID coactivators...
  59. Yue L, Karr T, Nathan D, Swift H, Srinivasan S, Lindquist S. Genetic analysis of viable Hsp90 alleles reveals a critical role in Drosophila spermatogenesis. Genetics. 1999;151:1065-79 pubmed
  60. Dynlacht B, Hoey T, Tjian R. Isolation of coactivators associated with the TATA-binding protein that mediate transcriptional activation. Cell. 1991;66:563-76 pubmed
    ..fractionated Drosophila basal factors reveals that activation by NTF-1 requires factors present in the endogenous TFIID fraction that are distinct from the purified TATA-binding protein (TBP)...
  61. Aguilar Fuentes J, Valadez Graham V, Reynaud E, Zurita M. TFIIH trafficking and its nuclear assembly during early Drosophila embryo development. J Cell Sci. 2006;119:3866-75 pubmed
    ..In addition, we present evidence that suggests that DNA helicase subunits XPB and XPD are assembled in the core when they are transported into the nucleus and are required for the onset of transcription. ..
  62. Pham A, Muller S, Sauer F. Mesoderm-determining transcription in Drosophila is alleviated by mutations in TAF(II)60 and TAF(II)110. Mech Dev. 1999;84:3-16 pubmed
    ..Here, we show that TBP-associated-factors (TAF(II)s) within the basal transcription factor TFIID mediate transcriptional activation by Dorsal and Twist...
  63. Wang Y, Duttke S, Chen K, Johnston J, Kassavetis G, Zeitlinger J, et al. TRF2, but not TBP, mediates the transcription of ribosomal protein genes. Genes Dev. 2014;28:1550-5 pubmed publisher
    ..Here we show that TBP (TATA box-binding protein)-related factor TRF2, but not TBP, is required for transcription of the TCT-dependent RP ..
  64. Choi T, Cho N, Oh Y, Yoo M, Matsukage A, Ryu Y, et al. The DNA replication-related element (DRE)-DRE-binding factor (DREF) system may be involved in the expression of the Drosophila melanogaster TBP gene. FEBS Lett. 2000;483:71-7 pubmed
    The TATA box binding protein (TBP) is a general transcription factor required for initiation by all three eukaryotic RNA polymerases...
  65. Li L, Arnosti D. Long- and short-range transcriptional repressors induce distinct chromatin states on repressed genes. Curr Biol. 2011;21:406-12 pubmed publisher
  66. Metcalf C, Wassarman D. Nucleolar colocalization of TAF1 and testis-specific TAFs during Drosophila spermatogenesis. Dev Dyn. 2007;236:2836-43 pubmed
    ..tTAFs are paralogs of generally expressed TAF subunits of transcription factor IID (TFIID)...
  67. Emili A, Greenblatt J, Ingles C. Species-specific interaction of the glutamine-rich activation domains of Sp1 with the TATA box-binding protein. Mol Cell Biol. 1994;14:1582-93 pubmed
    ..specifically and directly to the C-terminal evolutionarily conserved domain of the human TATA box-binding protein (TBP)...
  68. Davidson I. The genetics of TBP and TBP-related factors. Trends Biochem Sci. 2003;28:391-8 pubmed
  69. Hernández Hernández A, Ferrus A. Prodos is a conserved transcriptional regulator that interacts with dTAF(II)16 in Drosophila melanogaster. Mol Cell Biol. 2001;21:614-23 pubmed
    The transcription factor TFIID is a multiprotein complex that includes the TATA box binding protein (TBP) and a number of associated factors, TAF(II). Prodos (PDS) is a conserved protein that exhibits a histone fold domain (HFD)...
  70. Manley J, Um M, Li C, Ashali H. Mechanisms of transcriptional activation and repression can both involve TFIID. Philos Trans R Soc Lond B Biol Sci. 1996;351:517-26 pubmed
    ..A principal target appears to be TFIID, which consists of the TATA binding protein (TBP) and associated factors (TAFs)...
  71. Kingston R, Bunker C, Imbalzano A. Repression and activation by multiprotein complexes that alter chromatin structure. Genes Dev. 1996;10:905-20 pubmed
    ..This implies a regulation of the activity of these complexes in response to developmental cues and further implies that the work to fully understand these complexes will occupy a generation of scientists. ..
  72. Lam K, Mühlpfordt F, Vaquerizas J, Raja S, Holz H, Luscombe N, et al. The NSL complex regulates housekeeping genes in Drosophila. PLoS Genet. 2012;8:e1002736 pubmed publisher
    ..The observed Pol II reduction coincides with compromised binding of TBP and TFIIB to target promoters, indicating that the NSL complex is required for optimal recruitment of the pre-..
  73. Matta B, Bitner Mathé B, Alves Ferreira M. Getting real with real-time qPCR: a case study of reference gene selection for morphological variation in Drosophila melanogaster wings. Dev Genes Evol. 2011;221:49-57 pubmed publisher
    ..Overall, we identified and validated a pair of genes (RpL32 and Tbp) as being stably expressed in both experimental comparisons...
  74. Hochheimer A, Tjian R. Diversified transcription initiation complexes expand promoter selectivity and tissue-specific gene expression. Genes Dev. 2003;17:1309-20 pubmed
  75. Dynlacht B, Weinzierl R, Admon A, Tjian R. The dTAFII80 subunit of Drosophila TFIID contains beta-transducin repeats. Nature. 1993;363:176-9 pubmed
    A key component of the RNA polymerase II transcriptional apparatus, TFIID, is a multi-protein complex containing the TATA box-binding protein (TBP) and at least seven tightly associated factors (TAFs)...
  76. Trivedi A, Young L, Ouyang C, Johnson D, Sprague K. A TATA element is required for tRNA promoter activity and confers TATA-binding protein responsiveness in Drosophila Schneider-2 cells. J Biol Chem. 1999;274:11369-75 pubmed
    ..Together these results indicate that, in insect cells, a TATA-like element is specifically required to form functional TATA-binding protein-containing complexes that promote efficient transcription of tRNA genes. ..
  77. Sandaltzopoulos R, Becker P. Heat shock factor increases the reinitiation rate from potentiated chromatin templates. Mol Cell Biol. 1998;18:361-7 pubmed
    ..Well-documented transcription activation mechanisms, such as the recruitment of TFIID and TFIIB, control the early phases of preinitiation complex formation...
  78. Smale S, Jain A, Kaufmann J, Emami K, Lo K, Garraway I. The initiator element: a paradigm for core promoter heterogeneity within metazoan protein-coding genes. Cold Spring Harb Symp Quant Biol. 1998;63:21-31 pubmed
  79. Wright K, Tjian R. Wnt signaling targets ETO coactivation domain of TAF4/TFIID in vivo. Proc Natl Acad Sci U S A. 2009;106:55-60 pubmed publisher
    Understanding the diverse activities of the multisubunit core promoter recognition complex TFIID in vivo requires knowledge of how individual subunits contribute to overall functions of this TATA box-binding protein (TBP)/TBP-associated ..
  80. Purnell B, Gilmour D. Contribution of sequences downstream of the TATA element to a protein-DNA complex containing the TATA-binding protein. Mol Cell Biol. 1993;13:2593-603 pubmed
    ..The results suggest that specific contacts downstream of the TATA element could play a key role in establishing the transcriptional potential of a gene by contributing to the interaction of the TATA-binding protein. ..
  81. Weinzierl R, Ruppert S, Dynlacht B, Tanese N, Tjian R. Cloning and expression of Drosophila TAFII60 and human TAFII70 reveal conserved interactions with other subunits of TFIID. EMBO J. 1993;12:5303-9 pubmed
    ..of transcription initiation by RNA polymerase II requires TFIID, a multisubunit complex composed of the TATA binding protein (TBP) and at least seven tightly associated factors (TAFs)...
  82. Shopland L, Hirayoshi K, Fernandes M, Lis J. HSF access to heat shock elements in vivo depends critically on promoter architecture defined by GAGA factor, TFIID, and RNA polymerase II binding sites. Genes Dev. 1995;9:2756-69 pubmed
    ..Because the two other critical regions include sequences that are required for stable binding of TFIID in vitro, we examined the in vivo occupancy of the TATA elements in the transgenic promoters...
  83. Erokhin M, Davydova A, Kyrchanova O, Parshikov A, Georgiev P, Chetverina D. Insulators form gene loops by interacting with promoters in Drosophila. Development. 2011;138:4097-106 pubmed publisher
    ..Both insulators support basal activity of the yellow and white promoters in eyes. Thus, the ability of insulators to interact with promoters might play an important role in the regulation of basal gene transcription...
  84. Hoey T, Dynlacht B, Peterson M, Pugh B, Tjian R. Isolation and characterization of the Drosophila gene encoding the TATA box binding protein, TFIID. Cell. 1990;61:1179-86 pubmed
    ..transcription complex, we have cloned, expressed, and characterized the Drosophila gene encoding the TATA binding protein, dTFIID...
  85. Agelopoulos M, Mckay D, Mann R. Developmental regulation of chromatin conformation by Hox proteins in Drosophila. Cell Rep. 2012;1:350-9 pubmed
    ..The pattern of binding by GAGA factor and the variant histone H2Av suggest that they play a role in the regulation of Dll chromatin conformation in expressing and nonexpressing cell types, respectively. ..
  86. Weinzierl R, Dynlacht B, Tjian R. Largest subunit of Drosophila transcription factor IID directs assembly of a complex containing TBP and a coactivator. Nature. 1993;362:511-7 pubmed
    The TFIID complex consists of the TATA-binding protein (TBP) and associated factors (TAFs) serving to mediate transcriptional activation by promoter-specific regulators...
  87. Yokomori K, Zeidler M, Chen J, Verrijzer C, Mlodzik M, Tjian R. Drosophila TFIIA directs cooperative DNA binding with TBP and mediates transcriptional activation. Genes Dev. 1994;8:2313-23 pubmed
    ..of dTFIIA-L, which corresponds to the 20-kD species, independently interact weakly with the TATA-binding protein (TBP). In contrast, the holo-dTFIIA (L/S) binds TBP with high affinity...
  88. Dynan W, Gilman M. Transcriptional control and signal transduction: of soap-operas and reductionism. Trends Genet. 1993;9:154-6 pubmed
  89. Farmer G, Friedlander P, Colgan J, Manley J, Prives C. Transcriptional repression by p53 involves molecular interactions distinct from those with the TATA box binding protein. Nucleic Acids Res. 1996;24:4281-8 pubmed
    ..from recent studies have suggested that this activity is mediated via an interaction between p53 and the TATA box binding protein (TBP)...
  90. Goodrich J, Hoey T, Thut C, Admon A, Tjian R. Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB. Cell. 1993;75:519-30 pubmed
    Enhancement of RNA polymerase II transcription by the viral transactivator VP16 requires the TFIID complex, which consists of the TATA-binding protein (TBP) and TBP-associated factors (TAFs)...
  91. Kerr L, Ransone L, Wamsley P, Schmitt M, Boyer T, Zhou Q, et al. Association between proto-oncoprotein Rel and TATA-binding protein mediates transcriptional activation by NF-kappa B. Nature. 1993;365:412-9 pubmed
    The c-Rel protein is able to associate in vitro and in vivo with the TATA-binding protein (TBP) of the TFIID complex. Coexpression of TBP with c-Rel augments transactivation from the kappa B site in Drosophila Schneider cells...