Taf1

Summary

Gene Symbol: Taf1
Description: TBP-associated factor 1
Alias: BG:DS00004.13, CG17603, Dmel\CG17603, EfW1, SR3-5, TAF, TAF1, TAF200, TAF230, TAF250, TAF250/230, TAFII-250, TAFII250, TAF[II]250, TAF[[II]], TAF[[II]]230, TAF[[II]]250, TAF[[II]]250/230, TFIID, TFIID TAF250, Taf1p, Taf200, Taf230, Taf250, Taf[[II]]250, cel, cell, d230, dTAF230, dTAF250, dTAFII250, dTAF[[II]]230, dTAF[[II]]250, dmTAF1, dmTAF[[II]]230, l(3)84Ab, p230, CG17603-PA, CG17603-PB, CG17603-PC, CG17603-PD, CG17603-PE, TATA-associated factor 250, TBP-associated factor, TBP-associated factor 250, TBP-associated factor 250kD, TFIID 230 kda subunit, Taf1-PA, Taf1-PB, Taf1-PC, Taf1-PD, Taf1-PE, cell lethal, suppressor of Ras85D 3-5
Species: fruit fly

Top Publications

  1. pmc TAF250 is required for multiple developmental events in Drosophila
    D A Wassarman
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 97:1154-9. 2000
  2. ncbi Molecular cloning and characterization of dTAFII30 alpha and dTAFII30 beta: two small subunits of Drosophila TFIID
    K Yokomori
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Genes Dev 7:2587-97. 1993
  3. pmc Holo-TFIID controls the magnitude of a transcription burst and fine-tuning of transcription
    Katie L Pennington
    Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454, USA
    Proc Natl Acad Sci U S A 110:7678-83. 2013
  4. ncbi The novel regulator of metazoan development SAYP organizes a nuclear coactivator supercomplex
    Nadezhda E Vorobyeva
    Department of Regulation of Gene Expression, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia
    Cell Cycle 8:2152-6. 2009
  5. ncbi Reconstitution of chromatin transcription with purified components reveals a chromatin-specific repressive activity of p300
    Buyung Santoso
    Section of Molecular Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0347, USA
    Nat Struct Mol Biol 13:131-9. 2006
  6. doi Eye transformer is a negative regulator of Drosophila JAK/STAT signaling
    Jenni Kallio
    Laboratory of Experimental Immunology, Institute of Medical Technology, Tampere University, Tampere, Finland
    FASEB J 24:4467-79. 2010
  7. doi Lack of the Drosophila BEAF insulator proteins alters regulation of genes in the Antennapedia complex
    Swarnava Roy
    NIDDK Metabolic Diseases Branch, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Genet Genomics 285:113-23. 2011
  8. pmc Fine-scale genetic mapping of a hybrid sterility factor between Drosophila simulans and D. mauritiana: the varied and elusive functions of "speciation genes"
    Luciana O Araripe
    Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    BMC Evol Biol 10:385. 2010
  9. ncbi SAYP interacts with DHR3 nuclear receptor and participates in ecdysone-dependent transcription regulation
    Nadezhda E Vorobyeva
    Russian Academy of Sciences, Moscow, Russia
    Cell Cycle 10:1821-7. 2011
  10. ncbi In vivo synthesis of Taf1p lacking the TAF N-terminal domain using alternative transcription or translation initiation sites
    Koji Kasahara
    Division of Molecular and Cellular Biology, Graduate School of Integrated Science, Yokohama City University, 230 0045, Japan
    Genes Cells 9:709-21. 2004

Scientific Experts

  • Luciana O Araripe
  • ARNOLD BERK
  • B Pugh
  • C A Mizzen
  • Nadezhda E Vorobyeva
  • David A Wassarman
  • Rebeccah J Katzenberger
  • R Tjian
  • Swarnava Roy
  • Sofia G Georgieva
  • Elena N Nabirochkina
  • Matthew S Marengo
  • Kevin J Wright
  • Chad E Metcalf
  • Yulii V Shidlovskii
  • Julia V Nikolenko
  • Julia L Kuzmina
  • T Kokubo
  • Y Nakatani
  • Laszlo Tora
  • F Sauer
  • Katie L Pennington
  • S Georgieva
  • Jan A Veenstra
  • Johanna C Scheuermann
  • Jenni Kallio
  • Michael T Marr
  • Craig M Hart
  • Nataliya V Soshnikova
  • A D Pham
  • Robert Tjian
  • Mikhail S Klenov
  • Lev Usakin
  • Vladimir A Gvozdev
  • Buyung Santoso
  • D A Wassarman
  • James T Kadonaga
  • Kristy L Kenyon
  • Lyubov A Lebedeva
  • Tobias Maile
  • S Bagby
  • Koji Kasahara
  • Selen Muratoglu
  • J L Chen
  • Laura K Smoyer
  • D Liu
  • K Yokomori
  • F Schock
  • Boris A Leibovitch
  • M Horikoshi
  • Sarah B Kennett
  • R G Roeder
  • Gung Wei Chirn
  • Sharon K Marr
  • L A Pereira
  • C P Verrijzer
  • C H Wu
  • J M Park
  • Vladislav V Panov
  • Alexey N Krasnov
  • L Tora
  • E Nabirochkina
  • Nan Jiang
  • Robert K McGinty
  • Nga Ly-Hartig
  • Katarzyna Oktaba
  • Leena Maija Vanha-Aho
  • S R Albright
  • Leena Mäkinen
  • Tom W Muir
  • Sven Fraterman
  • Morag Armstrong
  • Olli Silvennoinen
  • J T Lis
  • Jurg Muller
  • Mika Ramet
  • Matthias Wilm
  • Susanna Valanne
  • Andres Gaytan de Ayala Alonso
  • Juha Grönholm
  • Henna Myllymäki
  • Marina R Kopantseva
  • R O Weinzierl
  • M Ikura
  • M D Rabenstein
  • Thomas Tuschl
  • Sergey A Lavrov
  • Alexei A Aravin
  • Alfredo Villasante
  • Yian Yee Tan

Detail Information

Publications76

  1. pmc TAF250 is required for multiple developmental events in Drosophila
    D A Wassarman
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 97:1154-9. 2000
    ..Studies in unicellular systems indicate that TAF250 is required for progression through G(1)/S of the cell cycle and repression of apoptosis...
  2. ncbi Molecular cloning and characterization of dTAFII30 alpha and dTAFII30 beta: two small subunits of Drosophila TFIID
    K Yokomori
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Genes Dev 7:2587-97. 1993
    ..Both dTAFII30 alpha and dTAFII30 beta are associated with TFIID via interactions with other TAFs, including dTAFII250, dTAFII150, and dTAFII110. In addition, dTAFII30 alpha also contacts dTBP...
  3. pmc Holo-TFIID controls the magnitude of a transcription burst and fine-tuning of transcription
    Katie L Pennington
    Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454, USA
    Proc Natl Acad Sci U S A 110:7678-83. 2013
    ..Recent evidence suggests that the role and composition of TFIID are more diverse than previously understood...
  4. ncbi The novel regulator of metazoan development SAYP organizes a nuclear coactivator supercomplex
    Nadezhda E Vorobyeva
    Department of Regulation of Gene Expression, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia
    Cell Cycle 8:2152-6. 2009
    ..is related to the conserved domain SAY, which assembles a nuclear supercomplex BTFly consisting of Brahma and TFIID coactivators...
  5. ncbi Reconstitution of chromatin transcription with purified components reveals a chromatin-specific repressive activity of p300
    Buyung Santoso
    Section of Molecular Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0347, USA
    Nat Struct Mol Biol 13:131-9. 2006
    ..Hence, the mechanism of transcriptional repression by p300 is distinct from that of histone H1, PARP-1 or Sir2. These findings reveal a novel chromatin-specific repressive function of p300...
  6. doi Eye transformer is a negative regulator of Drosophila JAK/STAT signaling
    Jenni Kallio
    Laboratory of Experimental Immunology, Institute of Medical Technology, Tampere University, Tampere, Finland
    FASEB J 24:4467-79. 2010
    ..In the gastrointestinal infection model, where JAK/STAT signaling is important for stem cell renewal, CG14225/ET RNAi was protective in vivo...
  7. doi Lack of the Drosophila BEAF insulator proteins alters regulation of genes in the Antennapedia complex
    Swarnava Roy
    NIDDK Metabolic Diseases Branch, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Genet Genomics 285:113-23. 2011
    ..A control gene, Dref, was not affected. A full understanding of the regulation of ANT-C genes during development will have to take the role of BEAF into account...
  8. pmc Fine-scale genetic mapping of a hybrid sterility factor between Drosophila simulans and D. mauritiana: the varied and elusive functions of "speciation genes"
    Luciana O Araripe
    Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    BMC Evol Biol 10:385. 2010
    ..In the current work we used marked P-element insertions as dominant markers to efficiently locate one genetic factor causing a severe reduction in fertility in hybrid males of Drosophila simulans and D. mauritiana...
  9. ncbi SAYP interacts with DHR3 nuclear receptor and participates in ecdysone-dependent transcription regulation
    Nadezhda E Vorobyeva
    Russian Academy of Sciences, Moscow, Russia
    Cell Cycle 10:1821-7. 2011
    ..interacts with DHR3 nuclear receptor and activates the corresponding genes by recruiting the BTFly (Brahma and TFIID) coactivator supercomplex. The knockdown of SAYP leads to a decrease in the level of DHR3-activated transcription...
  10. ncbi In vivo synthesis of Taf1p lacking the TAF N-terminal domain using alternative transcription or translation initiation sites
    Koji Kasahara
    Division of Molecular and Cellular Biology, Graduate School of Integrated Science, Yokohama City University, 230 0045, Japan
    Genes Cells 9:709-21. 2004
    ..of yeast TAND1 or TAND2 with the equivalent domain from a Drosophila homologue leads to accumulation of truncated Taf1p in yeast. This study demonstrates that these truncated Taf1p derivatives lack TAND...
  11. ncbi TAF1 activates transcription by phosphorylation of serine 33 in histone H2B
    Tobias Maile
    Department of Biochemistry, University of California Riverside, Riverside, CA 95121, USA
    Science 304:1010-4. 2004
    ..at evolutionarily conserved Ser33 (H2B-S33) by the carboxyl-terminal kinase domain (CTK) of the Drosophila TFIID subunit TAF1...
  12. ncbi Phenotype of the Triplo-lethal locus of Drosophila melanogaster and its suppression by hyperoxia
    Laura K Smoyer
    School of Biological Sciences, University of Nebraska, Lincoln 68588 0118, USA
    Genet Res 82:163-70. 2003
    ..We have examined Tpl-aneuploid embryos and find that, in both trisomics and monosomics, the midgut shows extensive cell death and the tracheae are abnormal. Shortly thereafter, all tissues die...
  13. pmc Two different Drosophila ADA2 homologues are present in distinct GCN5 histone acetyltransferase-containing complexes
    Selen Muratoglu
    Institute of Biochemistry, University of Szeged, Hungary
    Mol Cell Biol 23:306-21. 2003
    ..Furthermore, in vivo the two dADA2 proteins showed different localizations on polytene X chromosomes. These results, taken together, suggest that the two Drosophila ADA2 homologues are present in distinct GCN5-containing HAT complexes...
  14. pmc GAGA factor and the TFIID complex collaborate in generating an open chromatin structure at the Drosophila melanogaster hsp26 promoter
    Boris A Leibovitch
    Department of Biology, Washington University, St Louis, Missouri 63130, USA
    Mol Cell Biol 22:6148-57. 2002
    ..we have created flies with an hsp26/lacZ transgene wherein the entire DNA segment known to interact with the TFIID complex has been replaced by a random sequence...
  15. ncbi A unified nomenclature for TATA box binding protein (TBP)-associated factors (TAFs) involved in RNA polymerase II transcription
    Laszlo Tora
    Genes Dev 16:673-5. 2002
  16. ncbi Sp3 represses gene expression via the titration of promoter-specific transcription factors
    Sarah B Kennett
    Department of Anatomy, Physiological Sciences, and Radiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606, USA
    J Biol Chem 277:9780-9. 2002
    ..Regions of M2 required for physical interactions with five TATA box-associated factors (TAF(II)s) were mapped, and mutations that disrupt the interaction of M2 with two of these proteins, TAF(II)70 and TAF(..
  17. pmc TAF(II)170 interacts with the concave surface of TATA-binding protein to inhibit its DNA binding activity
    L A Pereira
    Department of Physiological Chemistry, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands
    Mol Cell Biol 21:7523-34. 2001
    The human RNA polymerase II transcription factor B-TFIID consists of TATA-binding protein (TBP) and the TBP-associated factor (TAF) TAF(II)170 and can rapidly redistribute over promoter DNA...
  18. pmc TAF4 nucleates a core subcomplex of TFIID and mediates activated transcription from a TATA-less promoter
    Kevin J Wright
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, 16 Barker Hall, CA 94720, USA
    Proc Natl Acad Sci U S A 103:12347-52. 2006
    Activator-dependent recruitment of TFIID initiates formation of the transcriptional preinitiation complex...
  19. ncbi Mediator, not holoenzyme, is directly recruited to the heat shock promoter by HSF upon heat shock
    J M Park
    National Creative Research Initiative Center for Genome Regulation, Department of Biochemistry, Yonsei University, 120 749, Seoul, South Korea
    Mol Cell 8:9-19. 2001
    ..Therefore, the activator-Mediator interaction likely underlies the initiation of signal transfer from enhancer-bound activators to the basal transcription machinery...
  20. pmc The novel transcription factor e(y)2 interacts with TAF(II)40 and potentiates transcription activation on chromatin templates
    S Georgieva
    Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, 117334 Moscow, Russia
    Mol Cell Biol 21:5223-31. 2001
    ..studies demonstrate that the major complex, including both proteins, appears to be distinct from TFIID. Furthermore, we provide genetic evidence suggesting that the carboxy terminus of dTAF(II)40 is important for ..
  21. pmc Analysis of core promoter sequences located downstream from the TATA element in the hsp70 promoter from Drosophila melanogaster
    C H Wu
    Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Mol Cell Biol 21:1593-602. 2001
    ..In contrast, the initiator cross-linked exclusively to dTAF230. Remarkably, dTAF230 cross-links approximately 10 times more efficiently to the nontranscribed strand than to the ..
  22. ncbi Transcription. New insights into an old modification
    C A Mizzen
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908, USA
    Science 289:2290-1. 2000
  23. ncbi Fly SIX-type homeodomain proteins Sine oculis and Optix partner with different cofactors during eye development
    Kristy L Kenyon
    Department of Ophthalmology, Harvard Medical School and Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114, USA
    Dev Dyn 234:497-504. 2005
    ....
  24. pmc Occupancy of the Drosophila hsp70 promoter by a subset of basal transcription factors diminishes upon transcriptional activation
    Lyubov A Lebedeva
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, Centre National de Recherche Scientifique, Unite Mixte de Recherche 7104, Universite Louis Pasteur de Strasbourg, BP 10142, Illkirch, France
    Proc Natl Acad Sci U S A 102:18087-92. 2005
    ..TATA-binding protein (TBP) and several TBP-associated factors (TAFs), TFIIB, TFIIF (RAP30), TFIIH (XPB), TBP-free/TAF-containg complex (GCN5 and TRRAP), and the Mediator complex subunit 13 before heat shock...
  25. pmc Histone H2A deubiquitinase activity of the Polycomb repressive complex PR-DUB
    Johanna C Scheuermann
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nature 465:243-7. 2010
    Polycomb group (PcG) proteins are transcriptional repressors that control processes ranging from the maintenance of cell fate decisions and stem cell pluripotency in animals to the control of flowering time in plants...
  26. doi What the loss of the hormone neuroparsin in the melanogaster subgroup of Drosophila can tell us about its function
    Jan A Veenstra
    Universite de Bordeaux, CNRS CNIC UMR 5228, Avenue des Facultes, 33400 Talence Cedex, France
    Insect Biochem Mol Biol 40:354-61. 2010
    ..Perhaps the combination of the development of a complete metamorphosis and a redundant role in reproduction made neuroparsin dispensable in some Drosophila species...
  27. pmc Transcription coactivator SAYP combines chromatin remodeler Brahma and transcription initiation factor TFIID into a single supercomplex
    Nadezhda E Vorobyeva
    Department of Regulation of Gene Expression, Institute of Gene Biology, Russian Academy of Sciences, Vavilov Street 34 5, Moscow, 119334 Russia
    Proc Natl Acad Sci U S A 106:11049-54. 2009
    ..consisting of the chromatin-remodeling factor Brahma (SWI/SNF) and the transcription initiation factor TFIID, named BTFly (Brahma and TFIID in one assembly)...
  28. ncbi Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex
    K Yokomori
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Genes Dev 7:2235-45. 1993
    ..While characterizing the Drosophila TFIID complex, we discovered that a 30-kD protein that cofractionated with dTFIID was homologous to the previously ..
  29. pmc Control of alternative splicing by signal-dependent degradation of splicing-regulatory proteins
    Rebeccah J Katzenberger
    Department of Pharmacology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53706, USA
    J Biol Chem 284:10737-46. 2009
    ..this mechanism, an RNA interference screen in Drosophila S2 cells was used to identify proteins that regulate TAF1 (TBP-associated factor 1) alternative splicing in response to activation of the ATR (ATM-RAD3-related) signaling ..
  30. pmc Wnt signaling targets ETO coactivation domain of TAF4/TFIID in vivo
    Kevin J Wright
    Howard Hughes Medical Institute, Li Ka Shing Center for Biomedical and Sciences, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 106:55-60. 2009
    Understanding the diverse activities of the multisubunit core promoter recognition complex TFIID in vivo requires knowledge of how individual subunits contribute to overall functions of this TATA box-binding protein (TBP)/TBP-associated ..
  31. pmc A DNA damage signal activates and derepresses exon inclusion in Drosophila TAF1 alternative splicing
    Matthew S Marengo
    University of Wisconsin School of Medicine and Public Health, Department of Pharmacology, Molecular and Cellular Pharmacology Program, Madison, WI 53706, USA
    RNA 14:1681-95. 2008
    ..of TAF1 pre-mRNA sequences in the alternative splicing mechanism, we developed a TAF1 minigene (miniTAF1) and an S2 cell splicing assay that recapitulated key aspects of CPT-induced alternative splicing of endogenous TAF1...
  32. ncbi Nucleolar colocalization of TAF1 and testis-specific TAFs during Drosophila spermatogenesis
    Chad E Metcalf
    University of Wisconsin School of Medicine and Public Health, Department of Biomolecular Chemistry, Madison, Wisconsin 53706, USA
    Dev Dyn 236:2836-43. 2007
    ..tTAFs are paralogs of generally expressed TAF subunits of transcription factor IID (TFIID)...
  33. pmc Repeat-associated siRNAs cause chromatin silencing of retrotransposons in the Drosophila melanogaster germline
    Mikhail S Klenov
    Department of Molecular Genetics of Cell, Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia
    Nucleic Acids Res 35:5430-8. 2007
    ..Our results provide evidence that germinal Piwi-associated short RNAs induce chromatin modifications of their targets...
  34. pmc Transcription of the 1.688 satellite DNA family is under the control of RNA interference machinery in Drosophila melanogaster ovaries
    Lev Usakin
    Department of Animal Molecular Genetics, Institute of Molecular Genetics, Moscow 123182, Russia
    Genetics 176:1343-9. 2007
    ..The spn-E(1) mutation causes an increase of the H3-AcK9 mark and TAF1 (a component of the polymerase II transcriptional complex) occupancy in the chromatin of autosomal pericentromeric ..
  35. ncbi A genetic screen supports a broad role for the Drosophila insulator proteins BEAF-32A and BEAF-32B in maintaining patterns of gene expression
    Swarnava Roy
    Department of Biological Sciences, Louisiana State University, 70803, LA, USA
    Mol Genet Genomics 277:273-86. 2007
    ..Because it is unlikely that insulator function is limited to eye development, the present results support the hypothesis that insulators play a widespread role in maintaining global transcription programs...
  36. pmc ATM and ATR pathways signal alternative splicing of Drosophila TAF1 pre-mRNA in response to DNA damage
    Rebeccah J Katzenberger
    University of Wisconsin School of Medicine and Public Health, Department of Pharmacology, Madison, WI 53706, USA
    Mol Cell Biol 26:9256-67. 2006
    ..TAF1 encodes a subunit of TFIID, which is broadly required for RNA polymerase II transcription...
  37. ncbi DNA binding properties of TAF1 isoforms with two AT-hooks
    Chad E Metcalf
    Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53706, USA
    J Biol Chem 281:30015-23. 2006
    TATA-binding protein-associated factor 1 (TAF1) is an essential component of the general transcription factor IID (TFIID), which nucleates assembly of the preinitiation complex for transcription by RNA polymerase II...
  38. ncbi Ubiquitin-activating/conjugating activity of TAFII250, a mediator of activation of gene expression in Drosophila
    A D Pham
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
    Science 289:2357-60. 2000
    ..A biochemical approach identified the Drosophila coactivator TAFII250, the central subunit within the general transcription factor TFIID, as a histone-specific ubiquitin-activating/..
  39. ncbi Control of gene expression through regulation of the TATA-binding protein
    B F Pugh
    Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, 6802, University Park, PA, USA
    Gene 255:1-14. 2000
    ..Once TBP is bound to DNA, factors such as TAF(II)250 and Mot1 induce TBP to dissociate, while other factors such as NC2 and the NOT complex convert the TBP/DNA ..
  40. ncbi TAF-like function of SV40 large T antigen
    B Damania
    Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, 19104 6142, USA
    Genes Dev 10:1369-81. 1996
    ..to epitope-tagged TBP, endogenous TBP, hTAF(II)100, hTAF(II)130, and hTAF(II)250, under conditions where holo-TFIID would be precipitated...
  41. ncbi Multiple TAFIIs directing synergistic activation of transcription
    F Sauer
    Science 270:1783-8. 1995
    ..A quadruple complex containing TATA binding protein (TBP), TAFII250, TAFII110, and TAFII60 mediated transcriptional synergism by BCD and HB, whereas triple TBP-TAFII complexes ..
  42. ncbi Drosophila 230-kD TFIID subunit, a functional homolog of the human cell cycle gene product, negatively regulates DNA binding of the TATA box-binding subunit of TFIID
    T Kokubo
    National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892
    Genes Dev 7:1033-46. 1993
    A Drosophila cDNA encoding the largest TFIID subunit (p230) was isolated using a degenerate oligodeoxynucleotide probe based on an amino acid sequence of the purified protein...
  43. ncbi The dTAFII80 subunit of Drosophila TFIID contains beta-transducin repeats
    B D Dynlacht
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Nature 363:176-9. 1993
    ..5). In addition, the largest subunit, dTAFII250, binds directly to TBP and links other TAFs to the complex...
  44. ncbi Largest subunit of Drosophila transcription factor IID directs assembly of a complex containing TBP and a coactivator
    R O Weinzierl
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Nature 362:511-7. 1993
    ..Here we report the cloning of Drosophila TAFII250 and the assembly of a partial complex containing recombinant TBP, TAFII110 and the C-terminal domain of TAFII250...
  45. ncbi Identification of TFIID components required for transcriptional activation by upstream stimulatory factor
    T Kokubo
    National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 268:17554-8. 1993
    A TATA box-binding initiation factor, TFIID, plays a central role in the transcriptional regulation by activators...
  46. pmc The Drosophila 110-kDa transcription factor TFIID subunit directly interacts with the N-terminal region of the 230-kDa subunit
    T Kokubo
    National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 90:5896-900. 1993
    Transcription initiation factor TFIID is a multimeric protein complex that plays a central role in transcriptional regulation by facilitating promoter responses to various activators...
  47. pmc Cloning and expression of Drosophila TAFII60 and human TAFII70 reveal conserved interactions with other subunits of TFIID
    R O Weinzierl
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California at Berkeley 94720
    EMBO J 12:5303-9. 1993
    ..Recombinant TAFII60/70 binds weakly to TBP and tightly to the largest subunit of TFIID, TAFII250. In the presence of TAFII60/70, TBP and TAFII250, a stable ternary complex is formed...
  48. ncbi Drosophila TAFII150: similarity to yeast gene TSM-1 and specific binding to core promoter DNA
    C P Verrijzer
    Howard Hughes Medical Institute, University of California, Berkeley 94720 3202
    Science 264:933-41. 1994
    ..Both the product of TSM-1 and dTAFII150 bind directly to TBP and dTAFII250, demonstrating a functional similarity between human and yeast TAFs...
  49. pmc Interaction between the N-terminal domain of the 230-kDa subunit and the TATA box-binding subunit of TFIID negatively regulates TATA-box binding
    T Kokubo
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 91:3520-4. 1994
    Transcription initiation factor TFIID plays a central role in transcriptional regulation. Drosophila TFIID is a multimeric protein consisting of the TATA box-binding polypeptide (TBP) and a number of tightly associated polypeptides...
  50. ncbi Assembly of recombinant TFIID reveals differential coactivator requirements for distinct transcriptional activators
    J L Chen
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720 3202
    Cell 79:93-105. 1994
    ..A minimal complex containing TBP and TAFII250 directs basal but not activator-responsive transcription...
  51. ncbi Molecular cloning and functional analysis of Drosophila TAF110 reveal properties expected of coactivators
    T Hoey
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Cell 72:247-60. 1993
    The general transcription factor TFIID is a multiprotein complex containing the TATA-binding protein and several associated factors (TAFs), some of which may function as coactivators that are essential for activated, but not basal, ..
  52. ncbi TAFs and TFIIA mediate differential utilization of the tandem Adh promoters
    S K Hansen
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720 3204, USA
    Cell 82:565-75. 1995
    ..We propose a mechanism for regulating differential promoter utilization during Drosophila development that involves the recognition of specific initiator elements by TAFs in the TFIID complex.
  53. ncbi Binding of TAFs to core elements directs promoter selectivity by RNA polymerase II
    C P Verrijzer
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California at Berkeley 94720 3204, USA
    Cell 81:1115-25. 1995
    ..A comparison of different partial TBP-TAF assemblages established that a trimeric TBP-TAFII250-TAFII150 complex is minimally required for efficient utilization of the initiator and downstream promoter ..
  54. ncbi Molecular cloning of Drosophila TFIID subunits
    T Kokubo
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
    Nature 367:484-7. 1994
    Transcription initiation factor TFIID is a multisubunit complex containing a TATA-box-binding factor (TFIID tau/TBP) and associated polypeptide factors (TAFs) with sizes ranging from M(r) approximately 20,000 to > 200,000...
  55. ncbi Defects in embryogenesis in mutants associated with the antennapedia gene complex of Drosophila melanogaster
    B T Wakimoto
    Dev Biol 102:147-72. 1984
    ..The mutant phenotypes suggest, in addition to ensuring proper segment identity, the wild-type alleles of the 84A-84B1,2 genes are necessary for normal segmentation and elongation of the germ band and normal head involution...
  56. pmc A screen for genes that function downstream of Ras1 during Drosophila eye development
    F D Karim
    Howard Hughes Medical Institute, University of California, Berkeley 94720 3200, USA
    Genetics 143:315-29. 1996
    b>Cell-fate specification of the R7 photoreceptor cell is controlled by the sevenless receptor tyrosine kinase (SevRTK) and Ras1, the Drosophila homologue of mammalian H-ras, K-ras and N-ras oncogenes...
  57. pmc Functional interaction between p53, the TATA-binding protein (TBP), andTBP-associated factors in vivo
    G Farmer
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Mol Cell Biol 16:4295-304. 1996
    The transcriptional activator p53 is known to interact with components of the general transcription factor TFIID in vitro...
  58. ncbi The TAF(II)250 subunit of TFIID has histone acetyltransferase activity
    C A Mizzen
    Department of Biology, University of Rochester, New York 14627, USA
    Cell 87:1261-70. 1996
    The transcription initiation factor TFIID is a multimeric protein complex composed of TATA box-binding protein (TBP) and many TBP-associated factors (TAF(II)s)...
  59. ncbi TFIIA-TAF regulatory interplay: NMR evidence for overlapping binding sites on TBP
    S Bagby
    Division of Molecular Biology, Ontario Cancer Institute, Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, Ont, Canada
    FEBS Lett 468:149-54. 2000
    ..Transcription factor IIA (TFIIA) stabilizes the TBP-promoter complex whereas the N-terminal domain of the largest TAF(II) inhibits TBP-promoter interaction...
  60. pmc Two novel Drosophila TAF(II)s have homology with human TAF(II)30 and are differentially regulated during development
    S Georgieva
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, F 67404 Illkirch Cedex, CU de Strasbourg, France
    Mol Cell Biol 20:1639-48. 2000
    b>TFIID is a multiprotein complex composed of the TATA binding protein (TBP) and TBP-associated factors (TAF(II)s)...
  61. ncbi Mesoderm-determining transcription in Drosophila is alleviated by mutations in TAF(II)60 and TAF(II)110
    A D Pham
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, Germany
    Mech Dev 84:3-16. 1999
    ..Here, we show that TBP-associated-factors (TAF(II)s) within the basal transcription factor TFIID mediate transcriptional activation by Dorsal and Twist...
  62. pmc Drosophila head segmentation factor buttonhead interacts with the same TATA box-binding protein-associated factors and in vivo DNA targets as human Sp1 but executes a different biological program
    F Schock
    Max Planck Institut fur biophysikalische Chemie, Abteilung Molekulare Entwicklungsbiologie, Am Fassberg, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 96:5061-5. 1999
    ....
  63. pmc TATA box-binding protein (TBP)-related factor 2 (TRF2), a third member of the TBP family
    M D Rabenstein
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720 3204, USA
    Proc Natl Acad Sci U S A 96:4791-6. 1999
    ..These findings suggest that metazoans have evolved multiple TBPs to accommodate the vast increase in genes and expression patterns during development and cellular differentiation...
  64. ncbi Identification of highly conserved amino-terminal segments of dTAFII230 and yTAFII145 that are functionally interchangeable for inhibiting TBP-DNA interactions in vitro and in promoting yeast cell growth in vivo
    T Kotani
    Division of Gene Function in Animals, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma, Nara 630 0101, Japan
    J Biol Chem 273:32254-64. 1998
    b>TFIID is a multiprotein complex composed of TBP and several TAFIIs...
  65. ncbi A genetic and developmental analysis of mutations in labial, a gene necessary for proper head formation in Drosophila melanogaster
    V K Merrill
    Department of Biology, Indiana University, Bloomington 47405
    Dev Biol 135:376-91. 1989
    ..Mutations in lab, like those in the Deformed and proboscipedia loci of the ANT-C, reveal a homoeotic phenotype only in the adult stage of the life cycle...
  66. pmc Drosophila TAF(II)230 and the transcriptional activator VP16 bind competitively to the TATA box-binding domain of the TATA box-binding protein
    J Nishikawa
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 94:85-90. 1997
    The transcription initiation factor TFIID, consisting of the TATA box-binding protein (TBP) and many TBP-associated factors (TAFs), plays a central role in both basal and activated transcription...
  67. ncbi A cascade of transcriptional control leading to axis determination in Drosophila
    D Niessing
    Abteilung Molekulare Entwick lungsbiologie, Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    J Cell Physiol 173:162-7. 1997
  68. pmc Adf-1 is a nonmodular transcription factor that contains a TAF-binding Myb-like motif
    G Cutler
    Howard Hughes Medical Institute, University of California, Berkeley 94720, USA
    Mol Cell Biol 18:2252-61. 1998
    ..Like many transcriptional activators, Adf-1 contains a TFIID-binding domain that can interact with specific TAF subunits...
  69. ncbi Solution structure of a TBP-TAF(II)230 complex: protein mimicry of the minor groove surface of the TATA box unwound by TBP
    D Liu
    Department of Medical Biophysics, University of Toronto, Ontario, Canada
    Cell 94:573-83. 1998
    General transcription factor TFIID consists of TATA box-binding protein (TBP) and TBP-associated factors (TAF(II)s), which together play a central role in both positive and negative regulation of transcription...
  70. ncbi The bromodomain revisited
    F Jeanmougin
    Trends Biochem Sci 22:151-3. 1997
  71. ncbi TAFs revisited: more data reveal new twists and confirm old ideas
    S R Albright
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720 3204, USA
    Gene 242:1-13. 2000
    ..Coordinating the interaction of these proteins is the basal transcription factor TFIID, which recognizes the core promoter and supplies a scaffolding upon which the rest of the transcriptional ..
  72. ncbi TBP-like factors come into focus
    A J Berk
    Molecular Biology Institute, University of California, Los Angeles 90095, USA
    Cell 103:5-8. 2000
  73. ncbi TAF(II)s mediate activation of transcription in the Drosophila embryo
    F Sauer
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720 3204, USA
    Cell 87:1271-84. 1996
    Mutations in the genes for two highly conserved TAFs, TAF(II)60 and TAF(II)110, reduce transcription of Bicoid-dependent target genes in vivo...
  74. pmc TAFII mutations disrupt Dorsal activation in the Drosophila embryo
    J Zhou
    Molecular and Cell Biology Department, Molecular and Cell Biology Department, University of California, Berkeley, 401 Barker Hall, Berkeley, CA 94720 3204, USA
    Proc Natl Acad Sci U S A 95:13483-8. 1998
    In this study, we present evidence that the Dorsal activator interacts with limiting amounts of the TFIID complex in the Drosophila embryo...

Research Grants34

  1. High Resolution Mapping of Function Elements in the Yeast Genome
    B Pugh; Fiscal Year: 2007
    ..Nucleosomal control of gene expression is highly conserved among animals, thus lessons learned in flies and worms are directly applicable to human systems. ..
  2. MECHANISM OF P53 SILENCING BY ADENOVIRUS E1B 55K PROTEIN
    ARNOLD BERK; Fiscal Year: 2007
    Adenovirus is an important model system for understanding basic aspects of cell function...
  3. Promoter Regulation in Response to Environmental Stress
    B Pugh; Fiscal Year: 2007
    ..transcriptional regulatory pathways, represented by two related components of the transcription machinery, TFIID and SAGA, respectively...
  4. High Resolution Mapping of Function Elements in the Yeast Genome
    B Pugh; Fiscal Year: 2009
    ..Nucleosomal control of gene expression is highly conserved among animals, thus lessons learned in flies and worms are directly applicable to human systems. ..
  5. Promoter Regulation in Response to Environmental Stress
    B Franklin Pugh; Fiscal Year: 2010
    ..Heat shock provides a rapid and simple programming event for the cell. Preliminary studies on this project have already revealed novel insights into gene regulation by demonstrating ..
  6. Genome-wide Regulation of the TATA Binding Protein
    B Franklin Pugh; Fiscal Year: 2010
    ..In particular, the interplay between chromatin and two major TBP delivery complexes (TFIID and SAGA) will be investigated...
  7. MECHANISM OF P53 SILENCING BY ADENOVIRUS E1B 55K PROTEIN
    Arnold J Berk; Fiscal Year: 2010
    Adenovirus is an important model system for understanding basic aspects of cell function...
  8. Genome-wide Regulation of the TATA Binding Protein
    B Pugh; Fiscal Year: 2009
    ..In particular, the interplay between chromatin and two major TBP delivery complexes (TFIID and SAGA) will be investigated...
  9. Genome-wide Regulation of the TATA Binding Protein
    B Pugh; Fiscal Year: 2007
    Two related multisubunit complexes, TFIID and SAGA, serve similar roles in eukaryotic transcriptional regulation. Both have multiple shared activities including regulation of the TATA binding protein TBP...
  10. BIOSYNTHESIS OF ADENOVIRUS EARLY RNA'S
    ARNOLD BERK; Fiscal Year: 2007
    ..abstract_text> ..
  11. MECHANISM OF P53 SILENCING BY ADENOVIRUS E1B 55K PROTEIN
    ARNOLD BERK; Fiscal Year: 1999
    ..Interactions with purified TFIID-TAFs will be analyzed by protein blotting and chemical cross-linking...
  12. REGULATION OF TBP DIMERS
    B Pugh; Fiscal Year: 2003
    ..Dimerization of TBP (and its multi-subunit counterpart, TFIID) prevents TBP from accessing promoter DNA, and thus prevents transcription complex assembly and ultimately ..
  13. MECHANISM OF P53 SILENCING BY ADENOVIRUS E1B 55K PROTEIN
    ARNOLD BERK; Fiscal Year: 2004
    ..In a p53-minus cell line, the principal defect in the replication of the E1B- 55K null mutant d11520 at 32 degrees is failure to ..
  14. Promoter Regulation in Response to Environmental Stress
    B Pugh; Fiscal Year: 2007
    ..Heat shock provides a rapid and simple programming event for the cell. Preliminary studies on this project have already revealed novel insights into gene regulation by demonstrating ..
  15. High Resolution Mapping of Function Elements in the Yeast Genome
    B Franklin Pugh; Fiscal Year: 2010
    ..cellular behavior, knowledge of the genomic organizational state of nucleosomes is key toward maintaining proper cell physiology and rectifying aberrant states...