Gene Symbol: Ssrp
Description: Structure specific recognition protein
Alias: CG4817, Cf5, Dmel\CG4817, DssRP, SSRP, SSRP1, dSSRP, dSSRP1, dSsrp, ssrp, structure specific recognition protein, CG4817-PA, Ssrp-PA, chorion factor, structure-specific recognition protein
Species: fruit fly
Products:     Ssrp

Top Publications

  1. Bruhn S, Housman D, Lippard S. Isolation and characterization of cDNA clones encoding the Drosophila homolog of the HMG-box SSRP family that recognizes specific DNA structures. Nucleic Acids Res. 1993;21:1643-6 pubmed
    Recently an HMG-box protein denoted SSRP1, for structure-specific recognition protein 1, has been discovered which binds to specific DNA structural elements such as the bent, unwound conformations that occur upon the formation of ..
  2. Soullier S, Jay P, Poulat F, Vanacker J, Berta P, Laudet V. Diversification pattern of the HMG and SOX family members during evolution. J Mol Evol. 1999;48:517-27 pubmed
    ..The only consistent groups that can be detected by our analysis are HMG box 1, vertebrate HMG box 2, insect SSRP, and plant HMG...
  3. Hashimoto M, Kodera N, Tsunaka Y, Oda M, Tanimoto M, Ando T, et al. Phosphorylation-coupled intramolecular dynamics of unstructured regions in chromatin remodeler FACT. Biophys J. 2013;104:2222-34 pubmed publisher
    ..These data lead to the conclusion that the inhibitory effect of nucleosome binding is ascribed to the increase in the probability of encounter between HMG and the phosphorylated IDR. ..
  4. Kwon S, Florens L, Swanson S, Washburn M, Abmayr S, Workman J. Heterochromatin protein 1 (HP1) connects the FACT histone chaperone complex to the phosphorylated CTD of RNA polymerase II. Genes Dev. 2010;24:2133-45 pubmed publisher
    ..HP1c interacts with the SSRP1 (structure-specific recognition protein 1) subunit and the intact FACT complex...
  5. Tsunaka Y, Toga J, Yamaguchi H, Tate S, Hirose S, Morikawa K. Phosphorylated intrinsically disordered region of FACT masks its nucleosomal DNA binding elements. J Biol Chem. 2009;284:24610-21 pubmed publisher
    ..Importantly, this control mechanism appears to support rapid chromatin transactions during early embryogenesis through the dephosphorylation of some sites in the maternally transmitted dSSRP1.
  6. Polo S, Almouzni G. Chromatin assembly: a basic recipe with various flavours. Curr Opin Genet Dev. 2006;16:104-11 pubmed
    ..New data highlight the importance of histone dimers as deposition entities for de novo nucleosome assembly and identify dedicated machineries involved in histone variant deposition. ..
  7. Yokoyama R, Pannuti A, Ling H, Smith E, Lucchesi J. A plasmid model system shows that Drosophila dosage compensation depends on the global acetylation of histone H4 at lysine 16 and is not affected by depletion of common transcription elongation chromatin marks. Mol Cell Biol. 2007;27:7865-70 pubmed
  8. Koltowska K, Apitz H, Stamataki D, Hirst E, Verkade H, Salecker I, et al. Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis. Development. 2013;140:1912-8 pubmed publisher
    ..Here, we report the identification of zebrafish mutants for the gene encoding Structure specific recognition protein 1a (Ssrp1a), which, together with Spt16, forms the FACT heterodimer...
  9. Nakayama T, Shimojima T, Hirose S. The PBAP remodeling complex is required for histone H3.3 replacement at chromatin boundaries and for boundary functions. Development. 2012;139:4582-90 pubmed publisher
    ..3 replacement to execute boundary functions. Our results provide new insight into the function of the trithorax group during development. ..

More Information


  1. Levine M, Begun D. Evidence of spatially varying selection acting on four chromatin-remodeling loci in Drosophila melanogaster. Genetics. 2008;179:475-85 pubmed publisher
    ..Here we describe sequence variation at seven such chromatin-remodeling loci, four of which (chd1, ssrp, chm, and glu) exhibit strong differentiation between tropical and temperate populations...
  2. Hsu T, King D, LaBonne C, Kafatos F. A Drosophila single-strand DNA/RNA-binding factor contains a high-mobility-group box and is enriched in the nucleolus. Proc Natl Acad Sci U S A. 1993;90:6488-92 pubmed
    ..The gene encoding the D. melanogaster homolog, DssRP, is developmentally regulated and is expressed most abundantly in ovaries (nurse cells in particular)...
  3. Nakayama T, Nishioka K, Dong Y, Shimojima T, Hirose S. Drosophila GAGA factor directs histone H3.3 replacement that prevents the heterochromatin spreading. Genes Dev. 2007;21:552-61 pubmed
    ..Based on these findings we propose that the GAGA factor and FACT-dependent replacement of Lys 9-methylated histone H3 by H3.3 counteracts the spreading of silent chromatin...
  4. Shimojima T, Okada M, Nakayama T, Ueda H, Okawa K, Iwamatsu A, et al. Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor. Genes Dev. 2003;17:1605-16 pubmed
    ..We show here that Drosophila FACT (facilitates chromatin transcription), a heterodimer of dSPT16 and dSSRP1, is associated with GAGA factor through its dSSRP1 subunit, binds to a nucleosome, and facilitates GAGA factor-..
  5. Kasai N, Tsunaka Y, Ohki I, Hirose S, Morikawa K, Tate S. Solution structure of the HMG-box domain in the SSRP1 subunit of FACT. J Biomol NMR. 2005;32:83-8 pubmed
  6. Saunders A, Werner J, Andrulis E, Nakayama T, Hirose S, Reinberg D, et al. Tracking FACT and the RNA polymerase II elongation complex through chromatin in vivo. Science. 2003;301:1094-6 pubmed
    ..Our observations are consistent with FACT being restricted to transcription that involves nucleosome disassembly mechanisms. ..
  7. Neumüller R, Richter C, Fischer A, Novatchkova M, Neumüller K, Knoblich J. Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. Cell Stem Cell. 2011;8:580-93 pubmed publisher
    ..and show that the alternatively spliced transcription factor Lola and the transcriptional elongation factors Ssrp and Barc control self-renewal in neuroblast lineages...
  8. Kelley D, Stokes D, Perry R. CHD1 interacts with SSRP1 and depends on both its chromodomain and its ATPase/helicase-like domain for proper association with chromatin. Chromosoma. 1999;108:10-25 pubmed
    ..We also present evidence for an in vivo interaction between CHD1 and a novel HMG box-containing protein, SSRP1, which involves an amino-terminal segment of CHD1 that does not include the chromodomain...
  9. Wisniewski J, Szewczuk Z, Petry I, Schwanbeck R, Renner U. Constitutive phosphorylation of the acidic tails of the high mobility group 1 proteins by casein kinase II alters their conformation, stability, and DNA binding specificity. J Biol Chem. 1999;274:20116-22 pubmed
    ..As the occurrence of acidic tails containing canonical casein kinase II phosphorylation sites is common to diverse HMG and other chromosomal proteins, our results are probably of general significance. ..
  10. Chen C, Bowers S, Lipinszki Z, Palladino J, Trusiak S, Bettini E, et al. Establishment of Centromeric Chromatin by the CENP-A Assembly Factor CAL1 Requires FACT-Mediated Transcription. Dev Cell. 2015;34:73-84 pubmed publisher
    ..This transcription depends on the CAL1 binding partner FACT, but not on CENP-A incorporation. Our work establishes RNAPII passage as a key step in chaperone-mediated CENP-A chromatin establishment and propagation. ..
  11. Sessa L, Bianchi M. The evolution of High Mobility Group Box (HMGB) chromatin proteins in multicellular animals. Gene. 2007;387:133-40 pubmed
    ..The organization of HMGB genes was very conserved during Metazoan evolution, with the only deviations appearing in Caenorhabditis and Dipteran (Drosophila and Anopheles) species. ..
  12. Lipinszki Z, Lefevre S, Savoian M, Singleton M, Glover D, Przewloka M. Centromeric binding and activity of Protein Phosphatase 4. Nat Commun. 2015;6:5894 pubmed publisher
    ..We also show that binding of Flfl to CENP-C is required to bring PP4 activity to centromeres to maintain CENP-C and attached core kinetochore proteins at chromosomes during mitosis. ..
  13. Boltengagen M, Huang A, Boltengagen A, Trixl L, Lindner H, Kremser L, et al. A novel role for the histone acetyltransferase Hat1 in the CENP-A/CID assembly pathway in Drosophila melanogaster. Nucleic Acids Res. 2016;44:2145-59 pubmed publisher
    ..Thus, our results point toward intriguing analogies between the complex processing pathways of newly synthesized CENP-A and canonical histones. ..
  14. Pedersen K, Kristensen T, Loeschcke V, Petersen B, Duus J, Nielsen N, et al. Metabolomic signatures of inbreeding at benign and stressful temperatures in Drosophila melanogaster. Genetics. 2008;180:1233-43 pubmed publisher