Spt5

Summary

Gene Symbol: Spt5
Description: Spt5
Alias: CG7626, DSIF, Dmel\CG7626, Dspt5, SPT5, dDSIF, dSpt5, spt5, Spt5, CG7626-PA, Spt5-PA, complementation group C
Species: fruit fly

Top Publications

  1. Grabbe C, Zervas C, Hunter T, Brown N, Palmer R. Focal adhesion kinase is not required for integrin function or viability in Drosophila. Development. 2004;131:5795-805 pubmed
    ..Despite this, overexpressed Fak56 is a potent inhibitor of integrins binding to the extracellular matrix, suggesting that Fak56 may play a subtle role in the negative regulation of integrin adhesion. ..
  2. Missra A, Gilmour D. Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex. Proc Natl Acad Sci U S A. 2010;107:11301-6 pubmed publisher
    ..Protein-RNA cross-linking reveals that Spt5, the largest subunit of DSIF, contacts the nascent RNA as the RNA emerges from the elongation complex...
  3. Ni Z, Schwartz B, Werner J, Suarez J, Lis J. Coordination of transcription, RNA processing, and surveillance by P-TEFb kinase on heat shock genes. Mol Cell. 2004;13:55-65 pubmed
    ..We propose that P-TEFb phosphorylation of Pol II CTD coordinates transcription elongation with 3' end processing, and failure to do so leads to rapid RNA degradation. ..
  4. Prabhakaran M, Kelley R. Mutations in the transcription elongation factor SPT5 disrupt a reporter for dosage compensation in Drosophila. PLoS Genet. 2012;8:e1003073 pubmed publisher
    ..for dosage compensation, we found that mutations in the universally conserved transcription elongation factor Spt5 lower MSL complex dependent expression from the miniwhite reporter gene in vivo...
  5. Jennings B, Shah S, Yamaguchi Y, Seki M, Phillips R, Handa H, et al. Locus-specific requirements for Spt5 in transcriptional activation and repression in Drosophila. Curr Biol. 2004;14:1680-4 pubmed
    ..We have identified a missense mutation (W049) in the gene encoding the transcriptional elongation factor Spt5 (reviewed in ) which, when homozygous in the maternal germ line, leads to defects in segmental patterning of the ..
  6. Kaplan C, Morris J, Wu C, Winston F. Spt5 and spt6 are associated with active transcription and have characteristics of general elongation factors in D. melanogaster. Genes Dev. 2000;14:2623-34 pubmed
    The Spt4, Spt5, and Spt6 proteins are conserved throughout eukaryotes and are believed to play critical and related roles in transcription...
  7. Gilchrist D, dos Santos G, Fargo D, Xie B, Gao Y, Li L, et al. Pausing of RNA polymerase II disrupts DNA-specified nucleosome organization to enable precise gene regulation. Cell. 2010;143:540-51 pubmed publisher
  8. Wu C, Yamaguchi Y, Benjamin L, Horvat Gordon M, Washinsky J, Enerly E, et al. NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila. Genes Dev. 2003;17:1402-14 pubmed
    NELF and DSIF collaborate to inhibit elongation by RNA polymerase IIa in extracts from human cells...
  9. Saunders A, Werner J, Andrulis E, Nakayama T, Hirose S, Reinberg D, et al. Tracking FACT and the RNA polymerase II elongation complex through chromatin in vivo. Science. 2003;301:1094-6 pubmed
    ..FACT displays kinetics of recruitment and of chromosome tracking in vivo similar to Pol II and elongation factors Spt5 and Spt6...

More Information

Publications33

  1. Andrulis E, Guzman E, Döring P, Werner J, Lis J. High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: roles in promoter proximal pausing and transcription elongation. Genes Dev. 2000;14:2635-49 pubmed
    Recent studies have demonstrated roles for Spt4, Spt5, and Spt6 in the regulation of transcriptional elongation in both yeast and humans...
  2. Mahoney M, Parks A, Ruddy D, Tiong S, Esengil H, Phan A, et al. Presenilin-based genetic screens in Drosophila melanogaster identify novel notch pathway modifiers. Genetics. 2006;172:2309-24 pubmed
    ..The predicted functions of these newly identified genes are consistent with extracellular matrix and vesicular trafficking mechanisms in Presenilin and Notch pathway regulation and suggest a novel role for gamma-tubulin in the pathway. ..
  3. Jennings B. Pausing for thought: disrupting the early transcription elongation checkpoint leads to developmental defects and tumourigenesis. Bioessays. 2013;35:553-60 pubmed publisher
    ..Analysis of these mutations has revealed that control of transcriptional pausing is critical for a diverse range of biological pathways essential for animal development and survival. ..
  4. Zhang Z, Wu C, Gilmour D. Analysis of polymerase II elongation complexes by native gel electrophoresis. Evidence for a novel carboxyl-terminal domain-mediated termination mechanism. J Biol Chem. 2004;279:23223-8 pubmed
    ..The gel distinguishes Pol IIA and Pol IIB containing complexes. The interaction of DSIF (Spt4/Spt5) with the elongation complexes can be readily detected, and this association is not dependent on the ..
  5. Li J, Liu Y, Rhee H, Ghosh S, Bai L, Pugh B, et al. Kinetic competition between elongation rate and binding of NELF controls promoter-proximal pausing. Mol Cell. 2013;50:711-22 pubmed publisher
  6. Qiu Y, Gilmour D. Identification of Regions in the Spt5 Subunit of DRB Sensitivity-inducing Factor (DSIF) That Are Involved in Promoter-proximal Pausing. J Biol Chem. 2017;292:5555-5570 pubmed publisher
    DRB sensitivity-inducing factor (DSIF or Spt4/5) is a conserved transcription elongation factor that both inhibits and stimulates transcription elongation in metazoans...
  7. Ni Z, Saunders A, Fuda N, Yao J, Suarez J, Webb W, et al. P-TEFb is critical for the maturation of RNA polymerase II into productive elongation in vivo. Mol Cell Biol. 2008;28:1161-70 pubmed
    ..Strikingly, all transcription factors tested (P-TEFb, Spt5, Spt6, and TFIIS) and RNA-processing factor CstF50 exited the body of the gene with kinetics indistinguishable from ..
  8. Li J, Gilmour D. Reconstitution of factor-dependent, promoter proximal pausing in Drosophila nuclear extracts. Methods Mol Biol. 2015;1276:133-52 pubmed publisher
    ..The radiolabeled RNAs are isolated by hybridization to a biotinylated oligonucleotide and captured on magnetic beads. We also describe the use of antibodies to investigate mechanistic aspects of promoter proximal pausing. ..
  9. Pereira A, Paro R. Pho dynamically interacts with Spt5 to facilitate transcriptional switches at the hsp70 locus. Epigenetics Chromatin. 2017;10:57 pubmed publisher
    ..We found that Pleiohomeotic (Pho), a DNA-binding PcG member, dynamically interacts with Spt5, an elongation factor...
  10. Eissenberg J, Shilatifard A, Dorokhov N, Michener D. Cdk9 is an essential kinase in Drosophila that is required for heat shock gene expression, histone methylation and elongation factor recruitment. Mol Genet Genomics. 2007;277:101-14 pubmed
    ..Binding of the RNA Polymerase II elongation factor ELL was reduced in knockdown chromosomes, suggesting that ELL is recruited to active polymerase via CTD phosphorylation. ..
  11. Ivaldi M, Karam C, Corces V. Phosphorylation of histone H3 at Ser10 facilitates RNA polymerase II release from promoter-proximal pausing in Drosophila. Genes Dev. 2007;21:2818-31 pubmed
    ..The heat-shock transcription factor, the promoter-paused form of Pol II (Pol IIo(ser5)), and the pausing factor DSIF (DRB sensitivity-inducing factor) are still present at the hsp70 loci in JIL-1-null mutants, whereas levels of the ..
  12. Chopra V, Hong J, Levine M. Regulation of Hox gene activity by transcriptional elongation in Drosophila. Curr Biol. 2009;19:688-93 pubmed publisher
    ..Stalled Pol II persists in tissues where Ubx and Abd-B are silenced by the PcG complex. We propose that stalling fosters both the rapid induction and precise silencing of Hox gene expression during development. ..
  13. Boucher L, Ouzounis C, Enright A, Blencowe B. A genome-wide survey of RS domain proteins. RNA. 2001;7:1693-701 pubmed
  14. Chopra V, Cande J, Hong J, Levine M. Stalled Hox promoters as chromosomal boundaries. Genes Dev. 2009;23:1505-9 pubmed publisher
    ..are dependent on general transcription factors that inhibit Pol II elongation, including components of the DSIF and NELF complexes...
  15. Papp B, Muller J. Histone trimethylation and the maintenance of transcriptional ON and OFF states by trxG and PcG proteins. Genes Dev. 2006;20:2041-54 pubmed
  16. Murawska M, Hassler M, Renkawitz Pohl R, Ladurner A, Brehm A. Stress-induced PARP activation mediates recruitment of Drosophila Mi-2 to promote heat shock gene expression. PLoS Genet. 2011;7:e1002206 pubmed publisher
    ..We suggest that stress-induced chromatin PARylation serves to rapidly attract factors that are required for an efficient and timely transcriptional response. ..
  17. Smith S, Petruk S, Sedkov Y, Cho E, Tillib S, Canaani E, et al. Modulation of heat shock gene expression by the TAC1 chromatin-modifying complex. Nat Cell Biol. 2004;6:162-7 pubmed
    ..3). The subsequent recruitment of several other factors, including Spt5, Spt6 and FACT, is believed to facilitate Pol II elongation through nucleosomes downstream of the start site...
  18. Doumanis J, Wada K, Kino Y, Moore A, Nukina N. RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. PLoS ONE. 2009;4:e7275 pubmed publisher
    ..Newly identified modifiers including genes related to nuclear transport, nucleotide processes, and signaling, may be involved in polyglutamine aggregate formation and Huntington disease cascades. ..
  19. Fay A, Misulovin Z, Li J, Schaaf C, Gause M, Gilmour D, et al. Cohesin selectively binds and regulates genes with paused RNA polymerase. Curr Biol. 2011;21:1624-34 pubmed publisher
    ..Cohesin, NELF, and Spt5 pausing and elongation factor knockdown experiments indicate that cohesin does not inhibit binding of polymerase to ..
  20. Lagha M, Bothma J, Esposito E, Ng S, Stefanik L, Tsui C, et al. Paused Pol II coordinates tissue morphogenesis in the Drosophila embryo. Cell. 2013;153:976-87 pubmed publisher
    ..We conclude that paused Pol II and transcriptional synchrony are essential for coordinating cell behavior during morphogenesis. ..
  21. Harvey R, Schuster E, Jennings B. Pleiohomeotic interacts with the core transcription elongation factor Spt5 to regulate gene expression in Drosophila. PLoS ONE. 2013;8:e70184 pubmed publisher
    ..b>Spt5 interacts directly with RNA polymerase II and has an essential role in establishing this checkpoint, and also for ..
  22. Wu C, Lee C, Fan R, Smith M, Yamaguchi Y, Handa H, et al. Molecular characterization of Drosophila NELF. Nucleic Acids Res. 2005;33:1269-79 pubmed
    NELF and DSIF act together to inhibit transcription elongation in vitro, and are implicated in causing promoter proximal pausing on the hsp70 gene in Drosophila. Here, further characterization of Drosophila NELF is provided...
  23. Breiling A, O Neill L, D Eliseo D, Turner B, Orlando V. Epigenome changes in active and inactive polycomb-group-controlled regions. EMBO Rep. 2004;5:976-82 pubmed
    ..These PcG-specific repressive marks are maintained by the action of the E(z) HMT, an enzyme that has an important role not only in establishing but also in maintaining PcG repression. ..
  24. Andrulis E, Werner J, Nazarian A, Erdjument Bromage H, Tempst P, Lis J. The RNA processing exosome is linked to elongating RNA polymerase II in Drosophila. Nature. 2002;420:837-41 pubmed
    ..assays of Drosophila nuclear extracts show that the exosome also associates with the elongation factor dSpt5 and RNA polymerase II...