Spc105R

Summary

Gene Symbol: Spc105R
Description: Spc105-related
Alias: CG11451, DmSpc105, Dmel\CG11451, KNL1, Spc105, dmKNL-1, dmSpc105R, spc105R, Spc105-related, CG11451-PA, CG11451-PB, Spc105R-PA, Spc105R-PB
Species: fruit fly

Top Publications

  1. Schittenhelm R, Chaleckis R, Lehner C. Intrakinetochore localization and essential functional domains of Drosophila Spc105. EMBO J. 2009;28:2374-86 pubmed publisher
    ..It is composed of more than eighty different proteins. Spc105 (also designated as Spc7, KNL-1 or Blinkin in different eukaryotes) is a comparatively large kinetochore protein, ..
  2. Przewloka M, Venkei Z, Bolanos Garcia V, Debski J, Dadlez M, Glover D. CENP-C is a structural platform for kinetochore assembly. Curr Biol. 2011;21:399-405 pubmed publisher
    ..We now find that all kinetochore proteins belonging to the KMN (KNL1/Spc105, the Mis12 complex, and the Ndc80 complex) network bind to the N-terminal part of Drosophila CENP-C...
  3. Schittenhelm R, Althoff F, Heidmann S, Lehner C. Detrimental incorporation of excess Cenp-A/Cid and Cenp-C into Drosophila centromeres is prevented by limiting amounts of the bridging factor Cal1. J Cell Sci. 2010;123:3768-79 pubmed publisher
    ..We demonstrate that the low amount of endogenous Cal1 prevents centromere expansion and mitotic kinetochore failure when Cenp-A/Cid and Cenp-C are present in excess. ..
  4. Venkei Z, Przewloka M, Ladak Y, Albadri S, Sossick A, Juhasz G, et al. Spatiotemporal dynamics of Spc105 regulates the assembly of the Drosophila kinetochore. Open Biol. 2012;2:110032 pubmed publisher
    ..We found that the nuclear exclusion of the Spc105/KNL1 protein during interphase prevents precocious assembly of the Mis12 complex...
  5. Unhavaithaya Y, Park E, Royzman I, Orr Weaver T. Drosophila embryonic cell-cycle mutants. G3 (Bethesda). 2013;3:1875-80 pubmed publisher
    ..Other mutations affecting the centromere protein CAL1 and the kinetochore protein Spc105R caused mitotic defects in the nervous system.
  6. Rošić S, Köhler F, Erhardt S. Repetitive centromeric satellite RNA is essential for kinetochore formation and cell division. J Cell Biol. 2014;207:335-49 pubmed publisher
    ..In conclusion, our data reveal that SAT III RNA is an integral part of centromere identity, adding RNA to the complex epigenetic mark at centromeres in flies. ..
  7. Rodrigues N, Lekomtsev S, Jananji S, Kriston Vizi J, Hickson G, Baum B. Kinetochore-localized PP1-Sds22 couples chromosome segregation to polar relaxation. Nature. 2015;524:489-92 pubmed publisher
  8. Das A, Shah S, Fan B, Paik D, DiSanto D, Hinman A, et al. Spindle Assembly and Chromosome Segregation Requires Central Spindle Proteins in Drosophila Oocytes. Genetics. 2016;202:61-75 pubmed publisher
    ..In combination our results support a model where the meiotic central spindle and associated proteins are essential for acentrosomal chromosome segregation. ..
  9. Liu Y, Petrovic A, Rombaut P, Mosalaganti S, Keller J, Raunser S, et al. Insights from the reconstitution of the divergent outer kinetochore of Drosophila melanogaster. Open Biol. 2016;6:150236 pubmed publisher
    ..In addition, two paralogues of the KMN subunit Nnf1 (Nnf1a and Nnf1b) are present. Finally, the Spc105R subunit, homologous to human Knl1/CASC5, underwent considerable sequence changes in comparison with other ..

More Information

Publications15

  1. Feijão T, Afonso O, Maia A, Sunkel C. Stability of kinetochore-microtubule attachment and the role of different KMN network components in Drosophila. Cytoskeleton (Hoboken). 2013;70:661-75 pubmed publisher
    ..involves a conserved super-complex of proteins, known as the KNL1Mis12Ndc80 (KMN) network, composed by the KNL1 (Spc105), Mis12, and Ndc80 complexes...
  2. Richter M, Poznanski J, Zdziarska A, Czarnocki Cieciura M, Lipinszki Z, Dadlez M, et al. Network of protein interactions within the Drosophila inner kinetochore. Open Biol. 2016;6:150238 pubmed publisher
    ..Thus, we conclude the organization of the Mis12-Nnf1 dimer confers upon the Mis12 complex a bipolar, elongated structure that is critical for kinetochore function. ..
  3. Przewloka M, Venkei Z, Glover D. Searching for Drosophila Dsn1 kinetochore protein. Cell Cycle. 2009;8:1292-3 pubmed
    ..Here we speculate that Drosophila Spc105R, a protein very much diverged from its counterparts in other species, might not only be playing the role of ..
  4. Lipinszki Z, Lefevre S, Savoian M, Singleton M, Glover D, Przewloka M. Centromeric binding and activity of Protein Phosphatase 4. Nat Commun. 2015;6:5894 pubmed publisher
    ..We also show that binding of Flfl to CENP-C is required to bring PP4 activity to centromeres to maintain CENP-C and attached core kinetochore proteins at chromosomes during mitosis. ..
  5. Radford S, Hoang T, GÅ‚uszek A, Ohkura H, McKim K. Lateral and End-On Kinetochore Attachments Are Coordinated to Achieve Bi-orientation in Drosophila Oocytes. PLoS Genet. 2015;11:e1005605 pubmed publisher
    ..We have examined oocytes lacking two kinetochore proteins, NDC80 and SPC105R, and a centromere-associated motor protein, CENP-E, to characterize the impact of kinetochore-microtubule ..
  6. Derive N, Landmann C, Montembault E, Claverie M, Pierre Elies P, Goutte Gattat D, et al. Bub3-BubR1-dependent sequestration of Cdc20Fizzy at DNA breaks facilitates the correct segregation of broken chromosomes. J Cell Biol. 2015;211:517-32 pubmed publisher
    ..We therefore propose that the Bub3-BubR1 complex on broken DNA inhibits the APC/C locally via the sequestration of Cdc20, thus promoting proper transmission of broken chromosomes. ..