Gene Symbol: shrb
Description: shrub
Alias: CG8055, DmSNF2, Dmel\CG8055, Dvps32, ESCRT, ESCRT-III, Shrb, Shrub, Snf7, Snf7/Vps32, Vps32, anon-WO0118547.180, vps32, vps32/snf7, shrub, CG8055-PA, Vps32, shrb-PA
Species: fruit fly

Top Publications

  1. El Amine N, Kechad A, Jananji S, Hickson G. Opposing actions of septins and Sticky on Anillin promote the transition from contractile to midbody ring. J Cell Biol. 2013;203:487-504 pubmed publisher
    ..Our findings highlight the relatedness of the CR and MR and suggest that membrane removal is coordinated with CR disassembly. ..
  2. Kobia F, Duchi S, Deflorian G, Vaccari T. Pharmacologic inhibition of vacuolar H+ ATPase reduces physiologic and oncogenic Notch signaling. Mol Oncol. 2014;8:207-20 pubmed publisher
    ..Our data support V-ATPase inhibition as a novel therapeutic approach to counteract tumor growth via signaling pathways regulated at the endo-lysosomal level. ..
  3. Vaccari T, Rusten T, Menut L, Nezis I, Brech A, Stenmark H, et al. Comparative analysis of ESCRT-I, ESCRT-II and ESCRT-III function in Drosophila by efficient isolation of ESCRT mutants. J Cell Sci. 2009;122:2413-23 pubmed publisher
    b>ESCRT proteins were initially isolated in yeast as a single functional set of conserved components controlling endosomal cargo sorting and multivesicular body (MVB) biogenesis...
  4. Dong B, Hannezo E, Hayashi S. Balance between apical membrane growth and luminal matrix resistance determines epithelial tubule shape. Cell Rep. 2014;7:941-50 pubmed publisher
    ..Here, we report that Drosophila mutants of the ESCRT III component Shrub/Vps32 exhibit a strikingly elongated sinusoidal tube phenotype...
  5. Hori K, Sen A, Kirchhausen T, Artavanis Tsakonas S. Synergy between the ESCRT-III complex and Deltex defines a ligand-independent Notch signal. J Cell Biol. 2011;195:1005-15 pubmed publisher
    ..ligase Deltex and Kurtz, the nonvisual ?-arrestin in Drosophila, we identified Shrub, a core component of the ESCRT-III complex as a key modulator of this synergy...
  6. Issman Zecharya N, Schuldiner O. The PI3K class III complex promotes axon pruning by downregulating a Ptc-derived signal via endosome-lysosomal degradation. Dev Cell. 2014;31:461-73 pubmed publisher
    ..By studying several PI(3)P binding proteins, we found that Hrs, a subunit of the ESCRT-0 complex, required for multivesicular body (MVB) maturation, is essential for normal pruning progression...
  7. Rusten T, Vaccari T, Lindmo K, Rodahl L, Nezis I, Sem Jacobsen C, et al. ESCRTs and Fab1 regulate distinct steps of autophagy. Curr Biol. 2007;17:1817-25 pubmed
    ..Genetic and ultrastructural analysis showed that subunits of endosomal sorting complex required for transport (ESCRT)-I, -II and -III, as well as their regulatory ATPase Vps4 and the endosomal PtdIns(3)P 5-kinase Fab1, all are ..
  8. Capalbo L, Montembault E, Takeda T, Bassi Z, Glover D, D Avino P. The chromosomal passenger complex controls the function of endosomal sorting complex required for transport-III Snf7 proteins during cytokinesis. Open Biol. 2012;2:120070 pubmed publisher the assembly of filaments comprising components of the endosomal sorting complex required for transport-III (ESCRT-III)...
  9. Sweeney N, Brenman J, Jan Y, Gao F. The coiled-coil protein shrub controls neuronal morphogenesis in Drosophila. Curr Biol. 2006;16:1006-11 pubmed evolutionarily conserved coiled-coil protein homologous to the yeast protein Snf7, a key component in the ESCRT-III (endosomal sorting complex required for transport) complex that is involved in the formation of endosomal ..

More Information


  1. Schneider M, Troost T, Grawe F, Martinez Arias A, Klein T. Activation of Notch in lgd mutant cells requires the fusion of late endosomes with the lysosome. J Cell Sci. 2013;126:645-56 pubmed publisher
    ..contrast, the pathway is activated at an earlier phase in mutants of the gene that encodes the ESCRT-III component Shrub, which is an interaction partner of Lgd...
  2. Michelet X, Djeddi A, Legouis R. Developmental and cellular functions of the ESCRT machinery in pluricellular organisms. Biol Cell. 2010;102:191-202 pubmed publisher
    ..Most ESCRT research has been carried out in vitro using models such as yeast and mammalian cells in culture...
  3. Parchure A, Vyas N, Ferguson C, Parton R, Mayor S. Oligomerization and endocytosis of Hedgehog is necessary for its efficient exovesicular secretion. Mol Biol Cell. 2015;26:4700-17 pubmed publisher
    ..Perturbations of ESCRT proteins have a selective effect on long-range Hh signaling in Drosophila wing imaginal discs...
  4. Mattissek C, Teis D. The role of the endosomal sorting complexes required for transport (ESCRT) in tumorigenesis. Mol Membr Biol. 2014;31:111-9 pubmed publisher
    The endosomal sorting complexes required for transport (ESCRT) are needed for three distinct cellular functions in higher eukaryotes: (i) Multivesicular body formation for the degradation of transmembrane proteins in lysosomes, (ii) ..
  5. Loncle N, Agromayor M, Martin Serrano J, Williams D. An ESCRT module is required for neuron pruning. Sci Rep. 2015;5:8461 pubmed publisher
    ..Furthermore we show that this ESCRT module requires a direct, in vivo, interaction between Shrub/CHMP4B and the accessory protein Myopic/HD-PTP.
  6. Irion U, St Johnston D. bicoid RNA localization requires specific binding of an endosomal sorting complex. Nature. 2007;445:554-8 pubmed
    ..Here we show that mutants in all subunits of the ESCRT-II complex (VPS22, VPS25 and VPS36) abolish the final Staufen-dependent step in bicoid mRNA localization...
  7. Eikenes Ã, Malerød L, Christensen A, Steen C, Mathieu J, Nezis I, et al. ALIX and ESCRT-III coordinately control cytokinetic abscission during germline stem cell division in vivo. PLoS Genet. 2015;11:e1004904 pubmed publisher
    ..Here we show that ALIX and the ESCRT-III component Shrub are required for completion of abscission during Drosophila female germline stem cell (fGSC) ..
  8. Drusenheimer N, Migdal B, Jäckel S, Tveriakhina L, Scheider K, Schulz K, et al. The Mammalian Orthologs of Drosophila Lgd, CC2D1A and CC2D1B, Function in the Endocytic Pathway, but Their Individual Loss of Function Does Not Affect Notch Signalling. PLoS Genet. 2015;11:e1005749 pubmed publisher
    ..of the Notch receptor and other transmembrane receptors and physically interacts with the ESCRT-III component Shrub/CHMP4...
  9. Szatm ri Z, Kis V, Lippai M, Hegedus K, Farag T, Lőrincz P, et al. Rab11 facilitates cross-talk between autophagy and endosomal pathway through regulation of Hook localization. Mol Biol Cell. 2014;25:522-31 pubmed publisher
    ..Thus induction of autophagy appears to promote autophagic flux by increased convergence with the endosomal pathway...
  10. Morawa K, Schneider M, Klein T. Lgd regulates the activity of the BMP/Dpp signalling pathway during Drosophila oogenesis. Development. 2015;142:1325-35 pubmed publisher
    ..Moreover, Lgd physically interacts with Shrub, a fundamental component of the ESCRT trafficking machinery, whose loss of function results in the activation of several signalling pathways...
  11. Palmer W, Jia D, Deng W. Cis-interactions between Notch and its ligands block ligand-independent Notch activity. elife. 2014;3: pubmed publisher
  12. Dong B, Miao G, Hayashi S. A fat body-derived apical extracellular matrix enzyme is transported to the tracheal lumen and is required for tube morphogenesis in Drosophila. Development. 2014;141:4104-9 pubmed publisher
    ..This process was defective in rab9 and shrub/vps32 mutants and in wild-type embryos treated with a secretory pathway inhibitor, leading to an abundant ..
  13. Tjota M, Lee S, Wu J, Williams J, Khanna M, Thomas G. Annexin B9 binds to ?(H)-spectrin and is required for multivesicular body function in Drosophila. J Cell Sci. 2011;124:2914-26 pubmed publisher
    ..These results indicate that AnxB9 and ?(H) are intimately involved in endosomal trafficking to the MVB and play a role in maintaining high-fidelity segregation of the apical and lateral domains. ..
  14. Matias N, Mathieu J, Huynh J. Abscission is regulated by the ESCRT-III protein shrub in Drosophila germline stem cells. PLoS Genet. 2015;11:e1004653 pubmed publisher
    ..Here, we characterized the function of the ESCRT-III protein Shrub during cytokinesis in germ cells undergoing a series of complete and incomplete divisions...
  15. Ahmad S, Sweeney S, Lee J, Sweeney N, Gao F. Genetic screen identifies serpin5 as a regulator of the toll pathway and CHMP2B toxicity associated with frontotemporal dementia. Proc Natl Acad Sci U S A. 2009;106:12168-73 pubmed publisher
    ..pathogenic mutations in CHMP2B, which encodes a component of the endosomal sorting complex required for transport (ESCRT-III), are associated with FTD linked to chromosome 3 (FTD3)...
  16. McMillan B, Tibbe C, Jeon H, Drabek A, Klein T, Blacklow S. Electrostatic Interactions between Elongated Monomers Drive Filamentation of Drosophila Shrub, a Metazoan ESCRT-III Protein. Cell Rep. 2016;16:1211-1217 pubmed publisher
    The endosomal sorting complex required for transport (ESCRT) is a conserved protein complex that facilitates budding and fission of membranes...
  17. Sheng Z, Yu L, Zhang T, Pei X, Li X, Zhang Z, et al. ESCRT-0 complex modulates Rbf-mutant cell survival by regulating Rhomboid endosomal trafficking and EGFR signaling. J Cell Sci. 2016;129:2075-84 pubmed publisher
    ..screen in Drosophila, we identified synthetic lethal interactions between mutations of fly Rb (rbf) and the ESCRT-0 components stam and hrs We show that inactivation of ESCRT-0 sensitizes rbf-mutant cells to undergo apoptosis ..
  18. Philips J, Porto M, Wang H, Rubin E, Perrimon N. ESCRT factors restrict mycobacterial growth. Proc Natl Acad Sci U S A. 2008;105:3070-5 pubmed publisher
    ..Using a Drosophila model of infection, we identify three host cell activities, Rab7, CG8743, and the ESCRT machinery, that modulate the mycobacterial phagosome...
  19. Juhasz G, Hill J, Yan Y, Sass M, Baehrecke E, Backer J, et al. The class III PI(3)K Vps34 promotes autophagy and endocytosis but not TOR signaling in Drosophila. J Cell Biol. 2008;181:655-66 pubmed publisher
    ..Our results suggest that Vps34 is regulated by TOR-dependent nutrient signals directly at sites of autophagosome formation. ..
  20. West R, Lu Y, Marie B, Gao F, Sweeney S. Rab8, POSH, and TAK1 regulate synaptic growth in a Drosophila model of frontotemporal dementia. J Cell Biol. 2015;208:931-47 pubmed publisher
    ..We identified mutations in Rab8 in a genetic screen for enhancement of an FTD phenotype associated with ESCRT-III dysfunction...
  21. Kanamori T, Yoshino J, Yasunaga K, Dairyo Y, Emoto K. Local endocytosis triggers dendritic thinning and pruning in Drosophila sensory neurons. Nat Commun. 2015;6:6515 pubmed publisher
    ..Further genetic analyses suggest that local endocytosis in proximal dendrites functions cooperatively with global endocytosis-mediated protein degradation pathways to promote dendrite pruning. ..
  22. Troost T, Jaeckel S, Ohlenhard N, Klein T. The tumour suppressor Lethal (2) giant discs is required for the function of the ESCRT-III component Shrub/CHMP4. J Cell Sci. 2012;125:763-76 pubmed publisher
    ..caused by loss of function of the tumour suppressor gene lethal (2) giant discs (lgd) or the function of the ESCRT complexes I-III result in the ligand-independent activation of the Notch pathway in all imaginal disc cells in ..
  23. Lone M, Kungl T, Koper A, Bottenberg W, KAMMERER R, Klein M, et al. The nuclear protein Waharan is required for endosomal-lysosomal trafficking in Drosophila. J Cell Sci. 2010;123:2369-74 pubmed publisher
    ..Together with data demonstrating that Wah acts earlier in the trafficking pathway than the Escrt-III component Drosophila Shrb (snf7 in Schizosaccharomyces pombe), our results indicate that Wah is essential for ..
  24. Aradhya R, Zmojdzian M, Da Ponte J, Jagla K. Muscle niche-driven Insulin-Notch-Myc cascade reactivates dormant Adult Muscle Precursors in Drosophila. elife. 2015;4: pubmed publisher
    ..Altogether, we report that Drosophila AMPs display homing behavior to muscle niche and that the niche-driven Insulin-Notch-dMyc cascade plays a key role in setting the activated state of AMPs. ..
  25. Shravage B, Hill J, Powers C, Wu L, Baehrecke E. Atg6 is required for multiple vesicle trafficking pathways and hematopoiesis in Drosophila. Development. 2013;140:1321-9 pubmed publisher
    ..Thus, like mammals, altered Atg6 function in flies causes hematopoietic abnormalities and lethality, and our data indicate that this is due to defects in multiple membrane trafficking processes. ..
  26. Gao F, Brenman J, Jan L, Jan Y. Genes regulating dendritic outgrowth, branching, and routing in Drosophila. Genes Dev. 1999;13:2549-61 pubmed
    ..These findings begin to reveal the molecular mechanisms controlling dendritic morphogenesis. ..
  27. van den Brink D, Banerji O, Tear G. Commissureless regulation of axon outgrowth across the midline is independent of Rab function. PLoS ONE. 2013;8:e64427 pubmed publisher
    ..In addition we reveal that axon outgrowth of many populations of neurons within the Drosophila central nervous system is also independent of Rab activity. ..
  28. Tognon E, Wollscheid N, Cortese K, Tacchetti C, Vaccari T. ESCRT-0 is not required for ectopic Notch activation and tumor suppression in Drosophila. PLoS ONE. 2014;9:e93987 pubmed publisher
    Multivesicular endosome (MVE) sorting depends on proteins of the Endosomal Sorting Complex Required for Transport (ESCRT) family...
  29. Sanchez C, Teixeira F, Czech B, Preall J, Zamparini A, Seifert J, et al. Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation. Cell Stem Cell. 2016;18:276-90 pubmed publisher
    ..Collectively, these results detail the extensive genetic networks that control stem cell homeostasis and highlight the intricate regulation of protein synthesis during differentiation. ..
  30. Matusek T, Wendler F, Polès S, Pizette S, D Angelo G, Fürthauer M, et al. The ESCRT machinery regulates the secretion and long-range activity of Hedgehog. Nature. 2014;516:99-103 pubmed publisher
    ..secretion in Drosophila wing imaginal discs is dependent on the endosomal sorting complex required for transport (ESCRT)...