Gene Symbol: shi
Description: shibire
Alias: CG18102, DNM3, DYN, Ddyn, Ddyn3, Ddyn4, Dmel\CG18102, Dyn, Shi, anon-WO0153538.12, anon-WO0153538.13, anon-WO0153538.14, dDyn, dyn, dynamin, l(1)VII, l(1)shi, shibere, shibire, CG18102-PA, CG18102-PB, CG18102-PC, CG18102-PE, CG18102-PF, CG18102-PG, CG18102-PH, CG18102-PI, CG18102-PJ, CG18102-PK, CG18102-PL, CG18102-PM, CG18102-PN, CG18102-PO, CG18102-PP, dynamin, shi-PA, shi-PB, shi-PC, shi-PE, shi-PF, shi-PG, shi-PH, shi-PI, shi-PJ, shi-PK, shi-PL, shi-PM, shi-PN, shi-PO, shi-PP, shibere, shibiri
Species: fruit fly
Products:     shi

Top Publications

  1. Akalal D, Wilson C, Zong L, Tanaka N, Ito K, Davis R. Roles for Drosophila mushroom body neurons in olfactory learning and memory. Learn Mem. 2006;13:659-68 pubmed
    ..We also expressed a temperature-sensitive shibire (shi) transgene in these neuron sets and examined the effects of disrupting synaptic vesicle recycling on ..
  2. Marie B, Sweeney S, Poskanzer K, Roos J, Kelly R, Davis G. Dap160/intersectin scaffolds the periactive zone to achieve high-fidelity endocytosis and normal synaptic growth. Neuron. 2004;43:207-19 pubmed
    ..dap160 mutant synapses have decreased levels of essential endocytic proteins, including dynamin, endophilin, synaptojanin, and AP180, while other markers of the active zone and periactive zone are generally ..
  3. Burke C, Waddell S. Remembering nutrient quality of sugar in Drosophila. Curr Biol. 2011;21:746-50 pubmed publisher
    ..Therefore, flies can rapidly learn to discriminate between sugars using a postingestive reward evaluation system, and they preferentially remember nutritious sugars. ..
  4. Schweisguth F. Regulation of notch signaling activity. Curr Biol. 2004;14:R129-38 pubmed
    ..Recent analyses of the cell biology of the Notch receptor have identified several fundamental mechanisms that contribute to regulate Notch signaling activity in space and time. ..
  5. Kitamoto T. Conditional disruption of synaptic transmission induces male-male courtship behavior in Drosophila. Proc Natl Acad Sci U S A. 2002;99:13232-7 pubmed
    ..A temperature-sensitive allele of the Drosophila dynamin gene shibire (shi(ts1)) was expressed by using the GAL4/UAS system to disrupt synaptic transmission from GAL4-positive neurons ..
  6. Isabel G, Pascual A, Preat T. Exclusive consolidated memory phases in Drosophila. Science. 2004;304:1024-7 pubmed
    ..We propose that ARM acts as a gating mechanism that ensures that LTM is formed only after repetitive and spaced training. ..
  7. Rogat A, Miller K. A role for myosin VI in actin dynamics at sites of membrane remodeling during Drosophila spermatogenesis. J Cell Sci. 2002;115:4855-65 pubmed
    ..In addition, we show that dynamin localizes to these actin structures and that when dynamin and myosin VI function are both impaired, major defects ..
  8. Awasaki T, Ito K. Engulfing action of glial cells is required for programmed axon pruning during Drosophila metamorphosis. Curr Biol. 2004;14:668-77 pubmed
    ..inhibition of cellular functions, including endocytosis, in glial cells via the temperature-sensitive allele of shibire both suppresses glial infiltration and varicosity elimination and induces a severe delay in axon pruning...
  9. Lecuit T. Junctions and vesicular trafficking during Drosophila cellularization. J Cell Sci. 2004;117:3427-33 pubmed
    ..The novel proteins Nullo and Slam, which are specifically induced during cellularization, represent developmental regulators of membrane growth during cellularization. ..

More Information


  1. Overstreet E, Fitch E, Fischer J. Fat facets and Liquid facets promote Delta endocytosis and Delta signaling in the signaling cells. Development. 2004;131:5355-66 pubmed
    ..We propose that Faf plays a role similar to that of Neur in the Delta signaling cells. By deubiquitinating Lqf, which enhances the efficiency of Delta internalization, Faf stimulates Delta signaling. ..
  2. Pinal N, Pichaud F. Dynamin- and Rab5-dependent endocytosis is required to prevent Drosophila photoreceptor degeneration. J Cell Sci. 2011;124:1564-70 pubmed publisher
    ..Finally, we show that interfering with Dynamin- and Rab5-dependent endocytosis leads to a phenotype that is undistinguishable from that of the ninaE-null mutant...
  3. Schnaitmann C, Garbers C, Wachtler T, Tanimoto H. Color discrimination with broadband photoreceptors. Curr Biol. 2013;23:2375-82 pubmed publisher
    ..Our findings show that receptors with a complex and broad spectral sensitivity can contribute to color vision and reveal that chromatic and achromatic circuits in the fly share common photoreceptors. ..
  4. Noguchi T, Miller K. A role for actin dynamics in individualization during spermatogenesis in Drosophila melanogaster. Development. 2003;130:1805-16 pubmed
    ..We also suggest that endocytosis and exocytosis might not be important for membrane remodeling. This system should be suitable for analysis of defects in male sterile mutants and for investigating other steps of spermatogenesis. ..
  5. Alekseyenko O, Lee C, Kravitz E. Targeted manipulation of serotonergic neurotransmission affects the escalation of aggression in adult male Drosophila melanogaster. PLoS ONE. 2010;5:e10806 pubmed publisher
    ..showed that acute impairment of DA and 5HT neurotransmission using expression of a temperature sensitive form of dynamin completely abolished mid- and high-level aggression...
  6. Shimizu H, Kawamura S, Ozaki K. An essential role of Rab5 in uniformity of synaptic vesicle size. J Cell Sci. 2003;116:3583-90 pubmed
    ..Furthermore, we electrophysiologically and behaviourally showed that the function of Rab5 is essential for efficient signal transmission across synapses. ..
  7. Mateus A, Gorfinkiel N, Schamberg S, Martinez Arias A. Endocytic and recycling endosomes modulate cell shape changes and tissue behaviour during morphogenesis in Drosophila. PLoS ONE. 2011;6:e18729 pubmed publisher
    ..We propose that the modulation of the balance between endocytosis and recycling can regulate cellular morphology and tissue deformations during morphogenesis. ..
  8. Luan H, Lemon W, Peabody N, Pohl J, Zelensky P, Wang D, et al. Functional dissection of a neuronal network required for cuticle tanning and wing expansion in Drosophila. J Neurosci. 2006;26:573-84 pubmed
  9. Wagh D, Rasse T, Asan E, Hofbauer A, Schwenkert I, Dürrbeck H, et al. Bruchpilot, a protein with homology to ELKS/CAST, is required for structural integrity and function of synaptic active zones in Drosophila. Neuron. 2006;49:833-44 pubmed
    ..We propose that BRP is critical for intact AZ structure and normal-evoked neurotransmitter release at chemical synapses of Drosophila. ..
  10. Andretic R, van Swinderen B, Greenspan R. Dopaminergic modulation of arousal in Drosophila. Curr Biol. 2005;15:1165-75 pubmed
    ..Simpler behaviors, such as sleep and locomotion, show graded responses that follow changes in dopamine level. ..
  11. Zars T. Short-term memories in Drosophila are governed by general and specific genetic systems. Learn Mem. 2010;17:246-51 pubmed publisher
    ..The distributed basis of learning in Drosophila implies that these systems were independently selected. ..
  12. Belenkaya T, Wu Y, Tang X, Zhou B, Cheng L, Sharma Y, et al. The retromer complex influences Wnt secretion by recycling wntless from endosomes to the trans-Golgi network. Dev Cell. 2008;14:120-31 pubmed
    ..Our findings link Wls and retromer functions in the same conserved Wnt secretion pathway. We propose that retromer influences Wnt secretion by recycling Wntless from endosomes to the trans-Golgi network (TGN). ..
  13. Verstreken P, Ly C, Venken K, Koh T, Zhou Y, Bellen H. Synaptic mitochondria are critical for mobilization of reserve pool vesicles at Drosophila neuromuscular junctions. Neuron. 2005;47:365-78 pubmed
    In a forward screen for genes affecting neurotransmission in Drosophila, we identified mutations in dynamin-related protein (drp1)...
  14. Acharya S, Laupsien P, Wenzl C, Yan S, Großhans J. Function and dynamics of slam in furrow formation in early Drosophila embryo. Dev Biol. 2014;386:371-84 pubmed publisher
    ..Following the onset of zygotic gene expression, Slam and Nullo together stabilise this mark and Slam triggers invagination of the cleavage furrow. ..
  15. Pocha S, Wassmer T, Niehage C, Hoflack B, Knust E. Retromer controls epithelial cell polarity by trafficking the apical determinant Crumbs. Curr Biol. 2011;21:1111-7 pubmed publisher
    ..Our data reveal a mechanism for Crb trafficking by retromer that is vital for maintaining Crb levels and localization. We also show a novel function for retromer in maintaining epithelial cell polarity. ..
  16. Gerber B, Tanimoto H, Heisenberg M. An engram found? Evaluating the evidence from fruit flies. Curr Opin Neurobiol. 2004;14:737-44 pubmed
    ..With regard to olfactory learning in flies, we argue that the notion of the olfactory memory trace being localized to the Kenyon cells of the mushroom bodies is a reasonable working hypothesis. ..
  17. Jekely G, Sung H, Luque C, Rørth P. Regulators of endocytosis maintain localized receptor tyrosine kinase signaling in guided migration. Dev Cell. 2005;9:197-207 pubmed
    ..Cbl and Sprint both regulate early steps of RTK endocytosis. Thus, a physiological role of RTK endocytosis is to ensure localized intracellular response to guidance cues by stimulating spatial restriction of signaling. ..
  18. Entchev E, Schwabedissen A, Gonzalez Gaitan M. Gradient formation of the TGF-beta homolog Dpp. Cell. 2000;103:981-91 pubmed
    ..Evidence is presented that long-range Dpp movement involves Dpp receptor and Dynamin functions. We also show that the rates of endocytic trafficking and degradation determine Dpp signaling range...
  19. Chang H, Hull M, Mellman I. The J-domain protein Rme-8 interacts with Hsc70 to control clathrin-dependent endocytosis in Drosophila. J Cell Biol. 2004;164:1055-64 pubmed
    By screening for mutants exhibiting interactions with a dominant-negative dynamin, we have identified the Drosophila homologue of receptor-mediated endocytosis (Rme) 8, a J-domain-containing protein previously shown to be required for ..
  20. Zhu Y, Nern A, Zipursky S, Frye M. Peripheral visual circuits functionally segregate motion and phototaxis behaviors in the fly. Curr Biol. 2009;19:613-9 pubmed publisher
  21. Schwaerzel M, Monastirioti M, Scholz H, Friggi Grelin F, Birman S, Heisenberg M. Dopamine and octopamine differentiate between aversive and appetitive olfactory memories in Drosophila. J Neurosci. 2003;23:10495-502 pubmed
    ..Our results suggest that in associative conditioning, different memories are formed of the same odor under different circumstances, and that they are linked to the respective motivational systems by their specific modulatory pathways...
  22. Pelissier A, Chauvin J, Lecuit T. Trafficking through Rab11 endosomes is required for cellularization during Drosophila embryogenesis. Curr Biol. 2003;13:1848-57 pubmed
    ..Part of the membrane stems from endocytosed apical material. Mutants in the endocytic regulators rab5 and shibire/dynamin inhibit basal-lateral membrane growth, and apical endocytosis is blocked in shibire mutants...
  23. Narayanan R, Leonard M, Song B, Schmid S, Ramaswami M. An internal GAP domain negatively regulates presynaptic dynamin in vivo: a two-step model for dynamin function. J Cell Biol. 2005;169:117-26 pubmed
    ..Point mutations in shibire, the Drosophila dynamin, cause temperature-sensitive (ts) defects in endocytosis...
  24. Marois E, Mahmoud A, Eaton S. The endocytic pathway and formation of the Wingless morphogen gradient. Development. 2006;133:307-17 pubmed
    ..The glypican Dally-like promotes Wingless spreading by unknown mechanisms, while Dynamin-dependent endocytosis is thought to restrict Wingless spread...
  25. Krashes M, Keene A, Leung B, Armstrong J, Waddell S. Sequential use of mushroom body neuron subsets during drosophila odor memory processing. Neuron. 2007;53:103-15 pubmed
  26. Fabian Fine R, Verstreken P, Hiesinger P, Horne J, Kostyleva R, Zhou Y, et al. Endophilin promotes a late step in endocytosis at glial invaginations in Drosophila photoreceptor terminals. J Neurosci. 2003;23:10732-44 pubmed
    ..Using dynamin;endo double-mutant photoreceptors, we provide further evidence that ultimately the function of Endophilin is ..
  27. Ng M, Roorda R, Lima S, Zemelman B, Morcillo P, Miesenbock G. Transmission of olfactory information between three populations of neurons in the antennal lobe of the fly. Neuron. 2002;36:463-74 pubmed
    ..These circuits may serve as dynamic templates that extract higher order features from afferent activity patterns. ..
  28. Ataman B, Ashley J, Gorczyca M, Ramachandran P, Fouquet W, Sigrist S, et al. Rapid activity-dependent modifications in synaptic structure and function require bidirectional Wnt signaling. Neuron. 2008;57:705-18 pubmed publisher
    ..We propose that activation of the postsynaptic Wg pathway is required for the assembly of the postsynaptic apparatus, while activation of the presynaptic Wg pathway regulates cytoskeletal dynamics. ..
  29. Levayer R, Pelissier Monier A, Lecuit T. Spatial regulation of Dia and Myosin-II by RhoGEF2 controls initiation of E-cadherin endocytosis during epithelial morphogenesis. Nat Cell Biol. 2011;13:529-40 pubmed publisher
    ..Here we show that E-cadherin polarity is controlled by the polarized regulation of clathrin- and dynamin-mediated endocytosis. Blocking E-cadherin endocytosis resulted in cell intercalation defects...
  30. Callejo A, Bilioni A, Mollica E, Gorfinkiel N, Andres G, Ibañez C, et al. Dispatched mediates Hedgehog basolateral release to form the long-range morphogenetic gradient in the Drosophila wing disk epithelium. Proc Natl Acad Sci U S A. 2011;108:12591-8 pubmed publisher
    ..We also show that Dlp is needed in Hh-producing cells to allow for Hh release and that Ihog, which has been previously described as an Hh coreceptor, anchors Hh to the basolateral part of the disk epithelium. ..
  31. Rohrbough J, Broadie K. Electrophysiological analysis of synaptic transmission in central neurons of Drosophila larvae. J Neurophysiol. 2002;88:847-60 pubmed
    ..Synaptic current amplitude and frequency were reduced in shibire conditional dynamin mutants and increased in dunce cAMP phosphodiesterase mutants...
  32. Chotard C, Salecker I. Neurons and glia: team players in axon guidance. Trends Neurosci. 2004;27:655-61 pubmed
    ..Equally, neurons provide crucial information to glia, supporting their migration and correct positioning. ..
  33. Winther A, Jiao W, Vorontsova O, Rees K, Koh T, Sopova E, et al. The dynamin-binding domains of Dap160/intersectin affect bulk membrane retrieval in synapses. J Cell Sci. 2013;126:1021-31 pubmed publisher
    b>Dynamin-associated protein 160 kDa (Dap160)/intersectin interacts with several synaptic proteins and affects endocytosis and synapse development. The functional role of the different protein interaction domains is not well understood...
  34. Vogt K, Schnaitmann C, Dylla K, Knapek S, Aso Y, Rubin G, et al. Shared mushroom body circuits underlie visual and olfactory memories in Drosophila. elife. 2014;3:e02395 pubmed publisher
    ..Thus, our results suggest that distinct sensory memories are processed in a common brain center. Such centralization of related brain functions is an economical design that avoids the repetition of similar circuit motifs. ..
  35. Bulgakova N, Grigoriev I, Yap A, Akhmanova A, Brown N. Dynamic microtubules produce an asymmetric E-cadherin-Bazooka complex to maintain segment boundaries. J Cell Biol. 2013;201:887-901 pubmed publisher
  36. Burke C, Huetteroth W, Owald D, Perisse E, Krashes M, Das G, et al. Layered reward signalling through octopamine and dopamine in Drosophila. Nature. 2012;492:433-7 pubmed publisher
    ..In addition, they reconcile previous findings with octopamine and dopamine and suggest that reinforcement systems in flies are more similar to mammals than previously thought. ..
  37. Rikhy R, Kumar V, Mittal R, Krishnan K. Endophilin is critically required for synapse formation and function in Drosophila melanogaster. J Neurosci. 2002;22:7478-84 pubmed
    ..Immunofluorescence studies show colocalization of endophilin with dynamin consistent with a possible role in synaptic vesicle recycling.
  38. Poskanzer K, Fetter R, Davis G. Discrete residues in the c(2)b domain of synaptotagmin I independently specify endocytic rate and synaptic vesicle size. Neuron. 2006;50:49-62 pubmed
  39. Krashes M, Dasgupta S, Vreede A, White B, Armstrong J, Waddell S. A neural circuit mechanism integrating motivational state with memory expression in Drosophila. Cell. 2009;139:416-27 pubmed publisher
    ..Therefore, dNPF and dopamine provide a motivational switch in the mushroom body that controls the output of appetitive memory...
  40. Villella A, Ferri S, Krystal J, Hall J. Functional analysis of fruitless gene expression by transgenic manipulations of Drosophila courtship. Proc Natl Acad Sci U S A. 2005;102:16550-7 pubmed
    ..males toward females and courtship among males when driving a conditional disrupter of synaptic transmission (shi(TS))...
  41. Broughton S, Kitamoto T, Greenspan R. Excitatory and inhibitory switches for courtship in the brain of Drosophila melanogaster. Curr Biol. 2004;14:538-47 pubmed
    ..Suppression of neurotransmission was accomplished by means of the dominantly acting, temperature-sensitive dynamin mutation shibire(ts1), whereas enhanced neuronal excitability was produced by means of a novel, dominantly acting,..
  42. Zhang Y, Rodesch C, Broadie K. Living synaptic vesicle marker: synaptotagmin-GFP. Genesis. 2002;34:142-5 pubmed
  43. Trannoy S, Redt Clouet C, Dura J, Preat T. Parallel processing of appetitive short- and long-term memories in Drosophila. Curr Biol. 2011;21:1647-53 pubmed publisher
    ..Taken together, these results demonstrate that appetitive short- and long-term memories are formed and processed in parallel. ..
  44. Yamaguchi S, Desplan C, Heisenberg M. Contribution of photoreceptor subtypes to spectral wavelength preference in Drosophila. Proc Natl Acad Sci U S A. 2010;107:5634-9 pubmed publisher
    ..Involvement of all photoreceptors [R1-R6, R7, R8 (blue), R8 (green)] distinguishes phototaxis from motion detection that is mediated exclusively by R1-R6. ..
  45. Rajan A, Tien A, Haueter C, Schulze K, Bellen H. The Arp2/3 complex and WASp are required for apical trafficking of Delta into microvilli during cell fate specification of sensory organ precursors. Nat Cell Biol. 2009;11:815-24 pubmed publisher
    ..Our data indicate that WASp-dependent Arp2/3 actin polymerization is crucial for apical presentation of Delta, providing a mechanistic link between actin polymerization and Notch signalling...
  46. Hoyer S, Eckart A, Herrel A, Zars T, Fischer S, Hardie S, et al. Octopamine in male aggression of Drosophila. Curr Biol. 2008;18:159-67 pubmed publisher
    ..Octopamine, a closely related biogenic amine, has been proposed to have a similar function in arthropods. However, the effect of octopamine on aggressive behavior is little understood...
  47. Keleman K, Vrontou E, Krüttner S, Yu J, Kurtovic Kozaric A, Dickson B. Dopamine neurons modulate pheromone responses in Drosophila courtship learning. Nature. 2012;489:145-9 pubmed publisher
    ..Our work thus reveals critical behavioural, cellular and molecular components of the learning rule by which Drosophila adjusts its innate mating strategy according to experience. ..
  48. Watts R, Hoopfer E, Luo L. Axon pruning during Drosophila metamorphosis: evidence for local degeneration and requirement of the ubiquitin-proteasome system. Neuron. 2003;38:871-85 pubmed
    ..Our findings suggest that some forms of axon pruning during development may share similarities with degeneration of axons in response to injury. ..
  49. Gallet A, Therond P. Temporal modulation of the Hedgehog morphogen gradient by a patched-dependent targeting to lysosomal compartment. Dev Biol. 2005;277:51-62 pubmed
    ..We further show that dynamin-dependent internalization of Hh is not regulating Hh spreading but is involved in shaping Hh gradient...
  50. Keene A, Stratmann M, Keller A, Perrat P, Vosshall L, Waddell S. Diverse odor-conditioned memories require uniquely timed dorsal paired medial neuron output. Neuron. 2004;44:521-33 pubmed
    ..These results suggest that DPM neurons are required to consolidate memory and are differently involved in memory of a volatile that requires multisensory integration. ..
  51. Classen A, Anderson K, Marois E, Eaton S. Hexagonal packing of Drosophila wing epithelial cells by the planar cell polarity pathway. Dev Cell. 2005;9:805-17 pubmed
    ..We propose that these proteins polarize trafficking of Cadherin-containing exocyst vesicles during junction remodeling. This may be a common mechanism for the action of planar cell polarity proteins in diverse systems. ..
  52. Leibfried A, Fricke R, Morgan M, Bogdan S, Bellaiche Y. Drosophila Cip4 and WASp define a branch of the Cdc42-Par6-aPKC pathway regulating E-cadherin endocytosis. Curr Biol. 2008;18:1639-48 pubmed publisher
    ..endocytic vesicles that emanate from the AJs; a phenotype that is also observed upon blocking vesicle scission in dynamin mutant cells...
  53. Dickman D, Lu Z, Meinertzhagen I, Schwarz T. Altered synaptic development and active zone spacing in endocytosis mutants. Curr Biol. 2006;16:591-8 pubmed
    ..Mutants in endophilin, synaptojanin, dynamin, AP180, and synaptotagmin all show increases in supernumerary bouton structures...
  54. Han C, Belenkaya T, Wang B, Lin X. Drosophila glypicans control the cell-to-cell movement of Hedgehog by a dynamin-independent process. Development. 2004;131:601-11 pubmed
    ..demonstrate that Hh movement in its receiving cells is regulated by a cell-to-cell mechanism that is independent of dynamin-mediated endocytosis. We propose that glypicans transfer Hh along the cell membrane to pattern a field of cells.
  55. Beronja S, Laprise P, Papoulas O, Pellikka M, Sisson J, Tepass U. Essential function of Drosophila Sec6 in apical exocytosis of epithelial photoreceptor cells. J Cell Biol. 2005;169:635-46 pubmed
    ..Furthermore, we show that Rab11 forms a complex with Sec5 and that Sec5 interacts with Sec6 suggesting that the exocyst is a Rab11 effector that facilitates protein transport to the apical rhabdomere in Drosophila PRCs. ..
  56. Doherty J, Logan M, Taşdemir O, Freeman M. Ensheathing glia function as phagocytes in the adult Drosophila brain. J Neurosci. 2009;29:4768-81 pubmed publisher
  57. Zhang K, Guo J, Peng Y, Xi W, Guo A. Dopamine-mushroom body circuit regulates saliency-based decision-making in Drosophila. Science. 2007;316:1901-4 pubmed
    ..Immunohistological analysis showed that mushroom bodies are densely innervated by dopaminergic axons. Thus, the circuit from the dopamine system to mushroom bodies is crucial for choice behavior in Drosophila. ..
  58. Guichet A, Wucherpfennig T, Dudu V, Etter S, Wilsch Bräuniger M, Hellwig A, et al. Essential role of endophilin A in synaptic vesicle budding at the Drosophila neuromuscular junction. EMBO J. 2002;21:1661-72 pubmed
    ..of the neuromuscular junction (NMJ) of mutant L3 larvae carrying the temperature-sensitive allele of dynamin, shibire. D-endoA null mutants show severe defects in motility and die at the early L2 larval stage...
  59. Wang Y, Chiang A, Xia S, Kitamoto T, Tully T, Zhong Y. Blockade of neurotransmission in Drosophila mushroom bodies impairs odor attraction, but not repulsion. Curr Biol. 2003;13:1900-4 pubmed
  60. Delgado R, Maureira C, Oliva C, Kidokoro Y, Labarca P. Size of vesicle pools, rates of mobilization, and recycling at neuromuscular synapses of a Drosophila mutant, shibire. Neuron. 2000;28:941-53 pubmed
    ..Using a temperature-sensitive mutant, shibire(ts), we studied pool sizes and vesicle mobilization rates...
  61. Pfeiffer B, Truman J, Rubin G. Using translational enhancers to increase transgene expression in Drosophila. Proc Natl Acad Sci U S A. 2012;109:6626-31 pubmed publisher
    ..multiple copies to be effective in single copy, including constructs expressing the temperature-sensitive inactivator of neuronal function Shibire(ts1), and for the use of cytoplasmic GFP to image the fine processes of neurons.
  62. Verstreken P, Ohyama T, Haueter C, Habets R, Lin Y, Swan L, et al. Tweek, an evolutionarily conserved protein, is required for synaptic vesicle recycling. Neuron. 2009;63:203-15 pubmed publisher
    ..Our data implicate Tweek in regulating synaptic vesicle recycling via an action mediated at least in part by the regulation of PI(4,5)P(2) levels or availability at the synapse. ..
  63. Hennig K, Colombani J, Neufeld T. TOR coordinates bulk and targeted endocytosis in the Drosophila melanogaster fat body to regulate cell growth. J Cell Biol. 2006;173:963-74 pubmed
    ..Our data indicate that endocytosis acts both as an effector function downstream of TOR and as a physiologically relevant regulator of TOR signaling...
  64. Gallet A, Staccini Lavenant L, Therond P. Cellular trafficking of the glypican Dally-like is required for full-strength Hedgehog signaling and wingless transcytosis. Dev Cell. 2008;14:712-25 pubmed publisher
    ..The cointernalization of Dlp with the Hh/Ptc complex is dynamin dependent and necessary for full-strength Hh signaling...
  65. Littleton J, Barnard R, Titus S, Slind J, Chapman E, Ganetzky B. SNARE-complex disassembly by NSF follows synaptic-vesicle fusion. Proc Natl Acad Sci U S A. 2001;98:12233-8 pubmed
    ..In addition, the entire vesicle pool can be depleted in shibire comatose double mutants, demonstrating that NSF activity is not required for the fusion step itself...
  66. van Swinderen B, McCartney A, Kauffman S, Flores K, Agrawal K, Wagner J, et al. Shared visual attention and memory systems in the Drosophila brain. PLoS ONE. 2009;4:e5989 pubmed publisher
  67. Pitman J, Huetteroth W, Burke C, Krashes M, Lai S, Lee T, et al. A pair of inhibitory neurons are required to sustain labile memory in the Drosophila mushroom body. Curr Biol. 2011;21:855-61 pubmed publisher
    ..We propose that APL neurons provide widespread inhibition to stabilize and maintain synaptic specificity of a labile memory trace in a recurrent DPM and MB α'β' neuron circuit. ..
  68. Potter C, Tasic B, Russler E, Liang L, Luo L. The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis. Cell. 2010;141:536-48 pubmed publisher
    ..The Q system can be expanded to other uses in Drosophila and to any organism conducive to transgenesis...
  69. Nitz D, van Swinderen B, Tononi G, Greenspan R. Electrophysiological correlates of rest and activity in Drosophila melanogaster. Curr Biol. 2002;12:1934-40 pubmed
  70. Chen C, Wu J, Lin H, Pai T, Fu T, Wu C, et al. Visualizing long-term memory formation in two neurons of the Drosophila brain. Science. 2012;335:678-85 pubmed publisher
    ..These findings suggest an extra-MB memory circuit in Drosophila: LTM consolidation (MB to DAL), storage (DAL), and retrieval (DAL to MB)...
  71. Kimura K, Hachiya T, Koganezawa M, Tazawa T, Yamamoto D. Fruitless and doublesex coordinate to generate male-specific neurons that can initiate courtship. Neuron. 2008;59:759-69 pubmed publisher
    ..Thus, the coordinated actions of two sex determination genes, dsx and fru, confer the unique ability to initiate male-typical sexual behavior on P1 neurons. ..
  72. Ghosh Roy A, Desai B, Ray K. Dynein light chain 1 regulates dynamin-mediated F-actin assembly during sperm individualization in Drosophila. Mol Biol Cell. 2005;16:3107-16 pubmed
    ..In addition, DDLC1 colocalizes with dynamin along investment cones and regulates F-actin assembly at this organelle by retaining dynamin along the cones...
  73. Lecuit T, Pilot F. Developmental control of cell morphogenesis: a focus on membrane growth. Nat Cell Biol. 2003;5:103-8 pubmed
    ..We suggest that tubulogenesis, tissue spreading and cell intercalation stem mostly from a remarkably small number of cell intrinsic surface remodelling events that confer on cells different modes of migratory behaviours. ..
  74. Su J, Chow B, Boulianne G, Wilde A. The BAR domain of amphiphysin is required for cleavage furrow tip-tubule formation during cellularization in Drosophila embryos. Mol Biol Cell. 2013;24:1444-53 pubmed publisher
    ..In contrast, dynamin activity is preferentially required for the formation of the subset of CFT-tubules containing the septin Peanut...
  75. Gallet A, Ruel L, Staccini Lavenant L, Therond P. Cholesterol modification is necessary for controlled planar long-range activity of Hedgehog in Drosophila epithelia. Development. 2006;133:407-18 pubmed
    ..We conclude that, as in vertebrates, cholesterol modification is essential for controlled long-range Hh signalling in Drosophila. ..
  76. Gao S, Takemura S, Ting C, Huang S, Lu Z, Luan H, et al. The neural substrate of spectral preference in Drosophila. Neuron. 2008;60:328-42 pubmed publisher
    ..We propose that Dm8 sacrifices spatial resolution for sensitivity by relaying signals from multiple R7s to projection neurons, which then provide output to higher visual centers. ..
  77. Ing B, Shteiman Kotler A, Castelli M, Henry P, Pak Y, Stewart B, et al. Regulation of Commissureless by the ubiquitin ligase DNedd4 is required for neuromuscular synaptogenesis in Drosophila melanogaster. Mol Cell Biol. 2007;27:481-96 pubmed
    ..These results demonstrate that dNedd4 and ubiquitination are required for Commissureless endocytosis and proper neuromuscular synaptogenesis. ..
  78. Kitamoto T. Targeted expression of temperature-sensitive dynamin to study neural mechanisms of complex behavior in Drosophila. J Neurogenet. 2002;16:205-28 pubmed
  79. Séjourné J, Plaçais P, Aso Y, Siwanowicz I, Trannoy S, Thoma V, et al. Mushroom body efferent neurons responsible for aversive olfactory memory retrieval in Drosophila. Nat Neurosci. 2011;14:903-10 pubmed publisher
    ..As the lateral horn has been implicated in innate responses to repellent odorants, we propose that MB-V2 neurons recruit the olfactory pathway involved in innate odor avoidance during memory retrieval. ..
  80. Yu D, Keene A, Srivatsan A, Waddell S, Davis R. Drosophila DPM neurons form a delayed and branch-specific memory trace after olfactory classical conditioning. Cell. 2005;123:945-57 pubmed
    ..DPM neurons are therefore "odor generalists" and form a delayed, branch-specific, and amnesiac-dependent memory trace that may guide behavior after acquisition. ..
  81. Stockinger P, Kvitsiani D, Rotkopf S, Tirián L, Dickson B. Neural circuitry that governs Drosophila male courtship behavior. Cell. 2005;121:795-807 pubmed
    ..This circuit includes olfactory neurons required for the behavioral response to sex pheromones. Anatomical differences in this circuit that might account for the dramatic differences in male and female sexual behavior are not apparent. ..
  82. Packard M, Koo E, Gorczyca M, Sharpe J, Cumberledge S, Budnik V. The Drosophila Wnt, wingless, provides an essential signal for pre- and postsynaptic differentiation. Cell. 2002;111:319-30 pubmed
    ..We suggest that Wg signals the coordinated development of pre- and postsynaptic compartments. ..
  83. Geisbrecht E, Montell D. Myosin VI is required for E-cadherin-mediated border cell migration. Nat Cell Biol. 2002;4:616-20 pubmed
    ..Multiple biochemical mechanisms may exist, therefore, for cadherins to associate with diverse unconventional myosins that are required for normal stereocilium formation or maintenance. ..
  84. Lee P, Lin H, Chang Y, Fu T, Dubnau J, Hirsh J, et al. Serotonin-mushroom body circuit modulating the formation of anesthesia-resistant memory in Drosophila. Proc Natl Acad Sci U S A. 2011;108:13794-9 pubmed publisher
    ..Thus, in addition to the Amnesiac peptide required for anesthesia-sensitive memory formation, the two DPM neurons also release 5HT acting on MB neurons for ARM formation...
  85. Kitamoto T. Conditional modification of behavior in Drosophila by targeted expression of a temperature-sensitive shibire allele in defined neurons. J Neurobiol. 2001;47:81-92 pubmed
    ..In this method, a temperature-sensitive allele of shibire (shi(ts1)) is overexpressed in neuronal subsets using the GAL4/UAS system...
  86. Starostina E, Liu T, Vijayan V, Zheng Z, Siwicki K, Pikielny C. A Drosophila DEG/ENaC subunit functions specifically in gustatory neurons required for male courtship behavior. J Neurosci. 2012;32:4665-74 pubmed publisher
    ..Furthermore, our work identifies a small subset of gustatory neurons with an essential role in activation of male courtship behavior, most likely in response to female pheromones. ..
  87. Fergestad T, Broadie K. Interaction of stoned and synaptotagmin in synaptic vesicle endocytosis. J Neurosci. 2001;21:1218-27 pubmed
    ..Both Stoned proteins colocalize precisely with endocytotic proteins including the AP2 complex and Dynamin in the "lattice network" characteristic of endocytotic domains in Drosophila presynaptic terminals...
  88. Majumdar A, Ramagiri S, Rikhy R. Drosophila homologue of Eps15 is essential for synaptic vesicle recycling. Exp Cell Res. 2006;312:2288-98 pubmed
    ..In addition, the temperature-sensitive paralytic defect of shibire mutant is enhanced by this mutant...
  89. Dawes Hoang R, Parmar K, Christiansen A, Phelps C, Brand A, Wieschaus E. folded gastrulation, cell shape change and the control of myosin localization. Development. 2005;132:4165-78 pubmed
    ..Our analysis of fog function therefore provides a direct link from patterning to cell shape change. ..
  90. Aso Y, Herb A, Ogueta M, Siwanowicz I, Templier T, Friedrich A, et al. Three dopamine pathways induce aversive odor memories with different stability. PLoS Genet. 2012;8:e1002768 pubmed publisher
    ..These results cast light on a cellular mechanism by which a noxious event induces different dopamine signals to a single brain structure to synthesize an aversive memory. ..
  91. Leventis P, Chow B, Stewart B, Iyengar B, Campos A, Boulianne G. Drosophila Amphiphysin is a post-synaptic protein required for normal locomotion but not endocytosis. Traffic. 2001;2:839-50 pubmed
    ..However, Drosophila Amphiphysin function is required in both larvae and adults for normal locomotion. ..