Genomes and Genes
Gene Symbol: shi
Alias: CG18102, DNM3, DYN, Ddyn, Ddyn3, Ddyn4, Dmel\CG18102, Dyn, Shi, anon-WO0153538.12, anon-WO0153538.13, anon-WO0153538.14, dDyn, dyn, l(1)VII, l(1)shi, shibere, CG18102-PA, CG18102-PB, CG18102-PC, CG18102-PE, CG18102-PF, CG18102-PG, CG18102-PH, CG18102-PI, CG18102-PJ, CG18102-PK, CG18102-PL, CG18102-PM, CG18102-PN, CG18102-PO, dynamin, shi-PA, shi-PB, shi-PC, shi-PE, shi-PF, shi-PG, shi-PH, shi-PI, shi-PJ, shi-PK, shi-PL, shi-PM, shi-PN, shi-PO, shibere, shibiri
Species: fruit fly
Publications109 found, 100 shown here
- Rolling blackout is required for synaptic vesicle exocytosisFu De Huang
Department of Biological Sciences, Kennedy Center for Research on Human Development, Brain Institute, Vanderbilt University, Nashville, Tennessee 37235 1634, USA
J Neurosci 26:2369-79. 2006..We conclude that RBO plays an essential role in neurotransmitter release, downstream of SV docking, likely mediating SV fusion...
- Myosin VI is required for E-cadherin-mediated border cell migrationErika R Geisbrecht
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 2185, USA
Nat Cell Biol 4:616-20. 2002..Multiple biochemical mechanisms may exist, therefore, for cadherins to associate with diverse unconventional myosins that are required for normal stereocilium formation or maintenance...
- Role of Drosophila Rab5 during endosomal trafficking at the synapse and evoked neurotransmitter releaseTanja Wucherpfennig
Max Planck Institut für molekulare Zellbiologie und Genetik, Dresden, Germany
J Cell Biol 161:609-24. 2003..vesicles pool is depleted, the endosome is also drastically reduced and only recovers from membrane derived by dynamin-mediated endocytosis...
- Dynamin self-assembles into rings suggesting a mechanism for coated vesicle buddingJ E Hinshaw
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037
Nature 374:190-2. 1995b>DYNAMIN, a 100K member of the GTPase superfamily, is the mammalian homologue of the Drosophila shibire gene product...
- Amphiphysin is necessary for organization of the excitation-contraction coupling machinery of muscles, but not for synaptic vesicle endocytosis in DrosophilaA Razzaq
Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK
Genes Dev 15:2967-79. 2001Amphiphysins 1 and 2 are enriched in the mammalian brain and are proposed to recruit dynamin to sites of endocytosis. Shorter amphiphysin 2 splice variants are also found ubiquitously, with an enrichment in skeletal muscle...
- Dynein light chain 1 regulates dynamin-mediated F-actin assembly during sperm individualization in DrosophilaAnindya Ghosh-Roy
Tata Institute of Fundamental Research, Mumbai 400 005, India
Mol Biol Cell 16:3107-16. 2005..In addition, DDLC1 colocalizes with dynamin along investment cones and regulates F-actin assembly at this organelle by retaining dynamin along the cones...
- Salience modulates 20-30 Hz brain activity in DrosophilaBruno van Swinderen
The Neurosciences Institute, 10640 John Jay Hopkins Drive, San Diego, California 92121, USA
Nat Neurosci 6:579-86. 2003..We demonstrated the association of these responses with behavioral tracking and initiated a genetic approach to investigating neural correlates of perception...
- Drosophila Amphiphysin is implicated in protein localization and membrane morphogenesis but not in synaptic vesicle endocytosisA C Zelhof
Institute of Neuroscience, HHMI, University of Oregon 1254, Eugene, OR 97403, USA
Development 128:5005-15. 2001..The strong misexpression phenotype coupled with the mild mutant and lack of phenotypes suggests that Amphiphysin acts redundantly with other proteins to organize specialized membrane domains within a diverse array of cell types...
- SNARE-complex disassembly by NSF follows synaptic-vesicle fusionJ T Littleton
Center for Learning and Memory and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 98:12233-8. 2001..In the absence of NSF activity, there are sufficient fusion-competent SNAREs to exocytose both the readily released and the reserve pool of synaptic vesicles...
- Essential role of endophilin A in synaptic vesicle budding at the Drosophila neuromuscular junctionAntoine Guichet
Laboratory of Developmental Biology, Institut Jacques Monod, 2 place Jussieu, F 75251 Paris Cedex 05, France
EMBO J 21:1661-72. 2002..of the neuromuscular junction (NMJ) of mutant L3 larvae carrying the temperature-sensitive allele of dynamin, shibire. D-endoA null mutants show severe defects in motility and die at the early L2 larval stage...
- Arrow (LRP6) and Frizzled2 cooperate to degrade Wingless in Drosophila imaginal discsEugenia Piddini
National Institute for Medical Research, The Ridgeway Mill Hill, London NW7 1AA, UK
Development 132:5479-89. 2005..Overall, Frizzled2 achieves ligand capture and internalization, whereas Arrow, and perhaps downstream signalling, are essential for lysosomal targeting...
- Male-specific fruitless specifies the neural substrates of Drosophila courtship behaviourDevanand S Manoli
Neurosciences Program, Stanford University, Stanford, California 94305, USA
Nature 436:395-400. 2005..Together, these data demonstrate that FruM proteins specify the neural substrates of male courtship...
- The role of Drosophila mushroom body signaling in olfactory memoryS E McGuire
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
Science 293:1330-3. 2001..neurotransmission through this region of the brain by expressing a temperature-sensitive allele of the shibire dynamin guanosine triphosphatase, which is required for synaptic transmission...
- Disruption of neurotransmission in Drosophila mushroom body blocks retrieval but not acquisition of memoryJ Dubnau
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
Nature 411:476-80. 2001..We have used a temperature-sensitive dynamin transgene, which disrupts synaptic transmission reversibly and on the time-scale of minutes, to investigate the ..
- Size of vesicle pools, rates of mobilization, and recycling at neuromuscular synapses of a Drosophila mutant, shibireR Delgado
Centro de Estudios Cientificos, Arturo Prat 514, Valdivia, Chile
Neuron 28:941-53. 2000..Vesicle recycling supports synaptic transmission during prolonged tetanic stimulation and the maximum recycling rate was 1000 vesicles/s...
- Interaction of stoned and synaptotagmin in synaptic vesicle endocytosisT Fergestad
Department of Biology, University of Utah, Salt Lake City, Utah 84112 0840, USA
J Neurosci 21:1218-27. 2001..Both Stoned proteins colocalize precisely with endocytotic proteins including the AP2 complex and Dynamin in the "lattice network" characteristic of endocytotic domains in Drosophila presynaptic terminals...
- A molecular pathway for light-dependent photoreceptor apoptosis in DrosophilaA Kiselev
Department of Pharmacology, UT Southwestern Medical Center, Dallas, Texas 75390, USA
Neuron 28:139-52. 2000..Together, these data define the proapoptotic molecules in Drosophila photoreceptors and indicate a novel signaling pathway for light-activated rhodopsin molecules in control of photoreceptor viability...
- The formation of stable rhodopsin-arrestin complexes induces apoptosis and photoreceptor cell degenerationP G Alloway
Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
Neuron 28:129-38. 2000..We propose that an identical mechanism may be responsible for the pathology found in a subset of human retinal degenerative disorders...
- The neural substrate of spectral preference in DrosophilaShuying Gao
Unit on Neuronal Connectivity, Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
Neuron 60:328-42. 2008..We propose that Dm8 sacrifices spatial resolution for sensitivity by relaying signals from multiple R7s to projection neurons, which then provide output to higher visual centers...
- Synapse scaffolding: intersection of endocytosis and growthKendal Broadie
Department of Biological Sciences, Kennedy Center for Research on Human Development, Vanderbilt University, Nashville, Tennessee 37232, USA
Curr Biol 14:R853-5. 2004The Drosophila dynamin-associated protein Dap160, homolog of the vertebrate Intersectins, is thought likely to act as a molecular scaffold in the synaptic periactive zone...
- Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivoKira E Poskanzer
Department of Biochemistry and Biophysics, Program in Neuroscience, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, California 94143, USA
Nature 426:559-63. 2003..By inactivating Syt I only during endocytosis, we demonstrate that Syt I is necessary for the endocytosis of synaptic vesicles that have undergone exocytosis using a functional Syt I protein...
- Endophilin promotes a late step in endocytosis at glial invaginations in Drosophila photoreceptor terminalsRuth Fabian-Fine
Neuroscience Institute, Department of Psychology, Life Sciences Centre, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4J1
J Neurosci 23:10732-44. 2003..Using dynamin;endo double-mutant photoreceptors, we provide further evidence that ultimately the function of Endophilin is ..
- Blockade of neurotransmission in Drosophila mushroom bodies impairs odor attraction, but not repulsionYalin Wang
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
Curr Biol 13:1900-4. 2003....
- The J-domain protein Rme-8 interacts with Hsc70 to control clathrin-dependent endocytosis in DrosophilaHenry C Chang
Dept of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, PO Box 208002, New Haven, CT 06520 8002, USA
J Cell Biol 164:1055-64. 2004By screening for mutants exhibiting interactions with a dominant-negative dynamin, we have identified the Drosophila homologue of receptor-mediated endocytosis (Rme) 8, a J-domain-containing protein previously shown to be required for ..
- Shibire mutations reveal distinct dynamin-independent and -dependent endocytic pathways in primary cultures of Drosophila hemocytesA Guha
National Center for Biological Sciences, Tata Institute of Fundamental Research, GKVK, Bangalore 560 065, India
J Cell Sci 116:3373-86. 2003..In primary culture, the endocytic phenotype of ALBR internalization in cells carrying mutations in Drosophila Dynamin (dDyn) at the shibire locus (shits) parallels the temperature-sensitive behavior of shits animals...
- Drosophila stoned proteins regulate the rate and fidelity of synaptic vesicle internalizationD T Stimson
Department of Molecular and Cellular Biology and Arizona Research Laboratories Division of Neurobiology, University of Arizona, Tucson, Arizona 85721, USA
J Neurosci 21:3034-44. 2001At an initial step during synaptic vesicle recycling, dynamin and adaptor proteins mediate the endocytosis of synaptic vesicle components from the plasma membrane...
- A role for actin dynamics in individualization during spermatogenesis in Drosophila melanogasterTatsuhiko Noguchi
Department of Biology, Washington University in St Louis, Campus Box 1229, 1 Brookings Drive, St Louis, MO 63130, USA
Development 130:1805-16. 2003..We also suggest that endocytosis and exocytosis might not be important for membrane remodeling. This system should be suitable for analysis of defects in male sterile mutants and for investigating other steps of spermatogenesis...
- Four-dimensional gene expression control: memories on the flyBenjamin Leung
Department of Neurobiology, University of Massachusetts Medical School, Worcester MA 01605, USA
Trends Neurosci 27:511-3. 2004..Two recent papers describe implementation of different but related technologies that now provide this missing element in fly behavioral research...
- Neural substrates of memory: from synapse to systemJosh Dubnau
Cold Spring Harbor Laboratories, 1 Bungtown Rd, Cold Spring Harbor, New York 11724, USA
J Neurobiol 54:238-53. 2003..Our objective here is to offer a conceptually unifying perspective and to discuss this perspective in relation to an experiment analysis of memory in Drosophila...
- Electrophysiological correlates of rest and activity in Drosophila melanogasterDouglas A Nitz
The Neurosciences Institute, 10640 John Jay Hopkins Drive, San Diego, CA 92121, USA
Curr Biol 12:1934-40. 2002....
- A role for myosin VI in actin dynamics at sites of membrane remodeling during Drosophila spermatogenesisAaron D Rogat
Department of Biology, Washington University, One Brookings Drive, St Louis, MO 63130, USA
J Cell Sci 115:4855-65. 2002..In addition, we show that dynamin localizes to these actin structures and that when dynamin and myosin VI function are both impaired, major defects ..
- Hsc70 is required for endocytosis and clathrin function in DrosophilaHenry C Chang
Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520 8002, USA
J Cell Biol 159:477-87. 2002..The Hsc70-4 mutation also interacted genetically with a dominant-negative mutant of dynamin, a gene required for the budding of clathrin-coated vesicles (CCVs)...
- Unique biochemical and behavioral alterations in Drosophila shibire(ts1) mutants imply a conformational state affecting dynamin subcellular distribution and synaptic vesicle cyclingMai Lei Chen
Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA
J Neurobiol 53:319-29. 2002b>Dynamin is a GTPase protein that is essential for clathrin-mediated endocytosis of synaptic vesicle membranes. The Drosophila dynamin mutation shi(ts1) changes a single residue (G273D) at the boundary of the GTPase domain...
- Conditional disruption of synaptic transmission induces male-male courtship behavior in DrosophilaToshihiro Kitamoto
Division of Neurosciences, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA 91010, USA
Proc Natl Acad Sci U S A 99:13232-7. 2002..A temperature-sensitive allele of the Drosophila dynamin gene shibire (shi(ts1)) was expressed by using the GAL4/UAS system to disrupt synaptic transmission from GAL4-..
- Endophilin is critically required for synapse formation and function in Drosophila melanogasterRicha Rikhy
Tata Institute of Fundamental Research, Mumbai 400 005 India
J Neurosci 22:7478-84. 2002..Immunofluorescence studies show colocalization of endophilin with dynamin consistent with a possible role in synaptic vesicle recycling.
- Lava lamp, a novel peripheral golgi protein, is required for Drosophila melanogaster cellularizationJ C Sisson
Department of Molecular, Cell and Developmental Biology, Sinsheimer Labs, University of California at Santa Cruz, Santa Cruz, California 95064, USA
J Cell Biol 151:905-18. 2000..have been previously implicated in cellularization and/or cytokinesis, including KLP3A, Anillin, Septins, and Dynamin. We now show that a novel MMAP, Lava Lamp (Lva), is also required for cellularization...
- Requirements for presenilin-dependent cleavage of notch and other transmembrane proteinsG Struhl
Department of Genetics and Development, Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA
Mol Cell 6:625-36. 2000..Hence, Notch, beta-APP, and perhaps other proteins may be targeted for Presenilin-mediated transmembrane cleavage by upstream processing events that sever the extracellular domain from the rest of the protein...
- Dopaminergic modulation of arousal in DrosophilaRozi Andretic
The Neuroscience Institute, San Diego, California 92121, USA
Curr Biol 15:1165-75. 2005..Drosophila behaviors have been studied that span different levels of arousal, from sleep to visual perception to psychostimulant responses...
- Endocytic trafficking of Wingless and its receptors, Arrow and DFrizzled-2, in the Drosophila wingAnna F Rives
University of Pennsylvania School of Medicine, Department of Cell and Developmental Biology, Philadelphia, PA 190104 6058, USA
Dev Biol 293:268-83. 2006..Thus, signal modulation by endocytic routing will depend on the tissue to be patterned and the goals during that patterning event...
- Bruchpilot, a protein with homology to ELKS/CAST, is required for structural integrity and function of synaptic active zones in DrosophilaDhananjay A Wagh
Lehrstuhl für Genetik und Neurobiologie, Theodor Boveri Institut für Biowissenschaften, Am Hubland, D 97074 Wurzburg, Germany
Neuron 49:833-44. 2006..We propose that BRP is critical for intact AZ structure and normal-evoked neurotransmitter release at chemical synapses of Drosophila...
- Role of Drosophila alpha-adaptin in presynaptic vesicle recyclingM Gonzalez-Gaitan
Max Planck Institut fur biophysikalische Chemie, Abt Molekulare Entwicklungsbiologie, Gottingen, Germany
Cell 88:767-76. 1997..In presynaptic terminals, alpha-adaptin defines a network-like membrane structure to which the GTPase dynamin is recruited...
- Discrete residues in the c(2)b domain of synaptotagmin I independently specify endocytic rate and synaptic vesicle sizeKira E Poskanzer
Department of Biochemistry and Biophysics, University of California, San Francisco, Rock Hall, 1550 Fourth Street, Box 2822, San Francisco, California 94158, USA
Neuron 50:49-62. 2006....
- Endophilin mutations block clathrin-mediated endocytosis but not neurotransmitter releasePatrik Verstreken
Program in Developmental Biology, Houston, TX, USA
Cell 109:101-12. 2002..We propose that kiss-and-run maintains neurotransmission at active zones of the larval NMJ in endophilin animals...
- Traffic of dynamin within individual Drosophila synaptic boutons relative to compartment-specific markersP S Estes
Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721, USA
J Neurosci 16:5443-56. 1996..trap an intermediate stage in synaptic vesicle recycling, we have examined the localization and redistribution of dynamin within single synaptic varicosities at the larval neuromuscular junction...
- Internalization is required for proper Wingless signaling in Drosophila melanogasterElaine S Seto
Department of Molecular and Human Genetics, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA
J Cell Biol 173:95-106. 2006..In Drosophila melanogaster cells stimulated with Wg media, dynamin or Rab5 knockdown causes reduced Super8XTOPflash activity, suggesting that internalization and endosomal ..
- Dap160/intersectin scaffolds the periactive zone to achieve high-fidelity endocytosis and normal synaptic growthBruno Marie
Department of Biochemistry and Biophysics, 1550 4th Street, GDBS Fourth Floor North, University of California, San Francisco, 94143, USA
Neuron 43:207-19. 2004..dap160 mutant synapses have decreased levels of essential endocytic proteins, including dynamin, endophilin, synaptojanin, and AP180, while other markers of the active zone and periactive zone are generally ..
- Reversible blockage of membrane retrieval and endocytosis in the garland cell of the temperature-sensitive mutant of Drosophila melanogaster, shibirets1T Kosaka
J Cell Biol 97:499-507. 1983..were investigated in a temperature-sensitive, single gene mutant of Drosophila melanogaster, shibirets1 (shi) and wild-type (Oregon-R)...
- Sequential use of mushroom body neuron subsets during drosophila odor memory processingMichael J Krashes
Department of Neurobiology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
Neuron 53:103-15. 2007....
- Reversible blockage of neurite development and growth cone formation in neuronal cultures of a temperature-sensitive mutant of DrosophilaY T Kim
Department of Biology, University of Iowa, Iowa City 52242
J Neurosci 7:3245-55. 1987....
- Multiple forms of dynamin are encoded by shibire, a Drosophila gene involved in endocytosisM S Chen
Cell Biology Group, Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545
Nature 351:583-6. 1991b>Dynamin was discovered in bovine brain tissue as a nucleotide-sensitive microtubule-binding protein of relative molecular mass 100,000...
- Dissection of the peripheral motion channel in the visual system of Drosophila melanogasterJens Rister
Lehrstuhl für Genetik und Neurobiologie, Universitat Wurzburg, Am Hubland, 97074, Wurzburg, Germany
Neuron 56:155-70. 2007..At low contrast, L1 and L2 depend upon each other for motion processing. Of the two minor pathways, amc/T1 specifically enhances the L1 pathway at intermediate contrast. L3 appears not to contribute to motion but to orientation behavior...
- Dynamin-like protein encoded by the Drosophila shibire gene associated with vesicular trafficA M van der Bliek
Division of Biology, California Institute of Technology, Pasadena 91125
Nature 351:411-4. 1991..The gene encodes a protein that is highly similar to rat dynamin, 69% of the amino-acid sequence is identical...
- Differential potencies of effector genes in adult DrosophilaAndreas S Thum
Lehrstuhl Genetik und Neurobiologie, Universität Würzburg Am Hubland Biozentrum, D 97074 Wurzburg, Germany
J Comp Neurol 498:194-203. 2006..Altogether, the efficiency of effector genes depends on properties of the targeted neurons. Thus, we conclude that the selection of the appropriate effector gene is critical for evaluating the function of neural circuits...
- Requirement for dynamin during Notch signaling in Drosophila neurogenesisL Seugnet
Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, Illkirch, France
Dev Biol 192:585-98. 1997..The loss of activity of dynamin, which is encoded by the gene shibire and is required for endocytosis, results in a similar phenotype...
- Two distinct pools of synaptic vesicles in single presynaptic boutons in a temperature-sensitive Drosophila mutant, shibireH Kuromi
Institute for Behavioral Sciences, Gunma University School of Medicine, Maebashi, Japan
Neuron 20:917-25. 1998..Cytochalasin D treatment eliminated the reserve pool and reduced synaptic transmission evoked by high frequency stimulation. Thus, the reserve pool may play a crucial role for sustaining high frequency synaptic transmission...
- Temperature-sensitive paralytic mutations demonstrate that synaptic exocytosis requires SNARE complex assembly and disassemblyJ T Littleton
Laboratory of Genetics, University of Wisconsin, Madison 53706, USA
Neuron 21:401-13. 1998..These results provide in vivo evidence that cycles of assembly and disassembly of SNARE complexes drive membrane trafficking at synapses...
- Synaptic mitochondria are critical for mobilization of reserve pool vesicles at Drosophila neuromuscular junctionsPatrik Verstreken
Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
Neuron 47:365-78. 2005In a forward screen for genes affecting neurotransmission in Drosophila, we identified mutations in dynamin-related protein (drp1)...
- Functional analysis of fruitless gene expression by transgenic manipulations of Drosophila courtshipAdriana Villella
Department of Biology, Brandeis University, Waltham, MA 02454, USA
Proc Natl Acad Sci U S A 102:16550-7. 2005..males toward females and courtship among males when driving a conditional disrupter of synaptic transmission (shi(TS))...
- The endocytic machinery in nerve terminals surrounds sites of exocytosisJ Roos
Department of Biochemistry and Biophysics, Hormone Research Institute, University of California, San Francisco, San Francisco, 94143 0534, USA
Curr Biol 9:1411-4. 1999....
- Directionality of wingless protein transport influences epidermal patterning in the Drosophila embryoM M Moline
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208 3500, USA
Development 126:4375-84. 1999..We have constructed a transgene expressing a dominant negative form of shibire (shi), the fly dynamin homologue...
- The endocytic pathway and formation of the Wingless morphogen gradientEric Marois
Max Planck Institute for Cell Biology and Genetics, Pfotenhauerstr 108, 01307 Dresden, Germany
Development 133:307-17. 2006..The glypican Dally-like promotes Wingless spreading by unknown mechanisms, while Dynamin-dependent endocytosis is thought to restrict Wingless spread...
- Genetic dissection of endocytic trafficking in Drosophila using a horseradish peroxidase-bride of sevenless chimera: hook is required for normal maturation of multivesicular endosomesA Sunio
Center for Basic Neuroscience, Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9111, USA
Mol Biol Cell 10:847-59. 1999..Our genetic analysis indicates that Hook is required late in endocytic trafficking to negatively regulate delivery from mature MVBs to multilammelar late endosomes and lysosomes...
- Synaptic vesicle size and number are regulated by a clathrin adaptor protein required for endocytosisB Zhang
Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
Neuron 21:1465-75. 1998..These results provide novel insights into the molecular mechanism of endocytosis and reveal a role for AP180 in regulating vesicle size through a clathrin-dependent reassembly process...
- Cellular identification of water gustatory receptor neurons and their central projection pattern in DrosophilaTsuyoshi Inoshita
Department of Biology, Graduate School of Sciences, Kyushu University, Ropponmatsu, Fukuoka 810 8560, Japan
Proc Natl Acad Sci U S A 103:1094-9. 2006..We show here that the water receptor neurons project to a specific region in the subesophageal ganglion, thus revealing the water taste sensory map in Drosophila...
- Induction of cAMP response element-binding protein-dependent medium-term memory by appetitive gustatory reinforcement in Drosophila larvaeKen Honjo
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305 8572, Japan
J Neurosci 25:7905-13. 2005..Moreover, we also show that synaptic output of larval mushroom body neurons is required for retrieval but not for acquisition and retention of the larval memory, including the CREB-dependent component...
- Awd, the homolog of metastasis suppressor gene Nm23, regulates Drosophila epithelial cell invasionGouthami Nallamothu
Medical University of South Carolina, Hollings Cancer Center, 86 Jonathan Lucas St, Room 330, Charleston, SC 29425, USA
Mol Cell Biol 28:1964-73. 2008..results in stalled migration and can revert the phenotype of overexpressing constitutive Pvr or dominant-negative dynamin. This is a rare example demonstrating the relevance of a metastasis suppressor gene function utilized in a ..
- Interpreting clone-mediated perturbations of morphogen profilesAvigdor Eldar
Department of Molecular Genetics, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel
Dev Biol 278:203-7. 2005..Our results provide rigorous criteria to examine existing data and can facilitate the design and interpretation of future clone experiments...
- Delivery of wingless to the ventral mesoderm by the developing central nervous system ensures proper patterning of individual slouch-positive muscle progenitorsVirginia T Cox
Program in Developmental Biology, Weill Graduate School of Medical Sciences at Cornell University and Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
Dev Biol 287:403-15. 2005..It also provides a means for one signaling pathway to be used reiteratively throughout development to impart unique positional and character information within a target field...
- Excitatory and inhibitory switches for courtship in the brain of Drosophila melanogasterSusan J Broughton
Department of Biology, New York University, New York, NY 10003, USA
Curr Biol 14:538-47. 2004..Much less is known of the more expansive, nondimorphic, but nonetheless essential, neural elements subserving male courtship behavior...
- Dopamine-mushroom body circuit regulates saliency-based decision-making in DrosophilaKe Zhang
Institute of Neuroscience, Key Laboratory of Neurobiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences CAS, 320 Yueyang Road, Shanghai 200031, China
Science 316:1901-4. 2007..Immunohistological analysis showed that mushroom bodies are densely innervated by dopaminergic axons. Thus, the circuit from the dopamine system to mushroom bodies is crucial for choice behavior in Drosophila...
- An internal GAP domain negatively regulates presynaptic dynamin in vivo: a two-step model for dynamin functionRadhakrishnan Narayanan
Department of Molecular and Cellular Biology and Arizona Research Laboratories Division of Neurobiology, University of Arizona, Tucson, AZ 85721, USA
J Cell Biol 169:117-26. 2005The mechanism by which the self-assembling GTPase dynamin functions in vesicle formation remains controversial. Point mutations in shibire, the Drosophila dynamin, cause temperature-sensitive (ts) defects in endocytosis...
- Temporal modulation of the Hedgehog morphogen gradient by a patched-dependent targeting to lysosomal compartmentArmel Gallet
Institute of Signaling, Developmental Biology and Cancer Research, CNRS UMR 6543, Centre de Biochimie, 06108 Nice Cedex 02, France
Dev Biol 277:51-62. 2005..We further show that dynamin-dependent internalization of Hh is not regulating Hh spreading but is involved in shaping Hh gradient...
- Retrograde signaling from the brain to the retina modulates the termination of the light response in DrosophilaShantadurga Rajaram
Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892 3736, USA
Proc Natl Acad Sci U S A 102:17840-5. 2005..Although the mechanism of this retrograde signaling remains to be discerned, the phenomenon establishes a previously unappreciated mode of control of the temporal responsiveness of a primary sensory neuron...
- Arousal mechanisms: speedy flies don't sleep at nightSerge Birman
IBDM Developmental Biology Institute, CNRS Inserm Université de la Méditerranée, Marseille, France
Curr Biol 15:R511-3. 2005..Fruit flies similarly use dopamine for arousal control, indicating an ancient evolutionary origin of this essential feature of the functioning brain...
- Essential function of Drosophila Sec6 in apical exocytosis of epithelial photoreceptor cellsSlobodan Beronja
Department of Zoology, University of Toronto, Toronto, Ontario M5S 3G5, Canada
J Cell Biol 169:635-46. 2005..Furthermore, we show that Rab11 forms a complex with Sec5 and that Sec5 interacts with Sec6 suggesting that the exocyst is a Rab11 effector that facilitates protein transport to the apical rhabdomere in Drosophila PRCs...
- radish encodes a phospholipase-A2 and defines a neural circuit involved in anesthesia-resistant memoryAnn Shyn Chiang
National Tsing Hua University, Institute of Biotechnology, Hsinchu, Taiwan
Curr Biol 14:263-72. 2004..In contrast, LTM is normal in radish mutants but is disrupted in CREB repressor transgenic flies. To date, nothing is known about the molecular, genetic, or cell biological pathways underlying ARM...
- A temperature-sensitive allele of Drosophila sesB reveals acute functions for the mitochondrial adenine nucleotide translocase in synaptic transmission and dynamin regulationRicha Rikhy
Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai 400005, India
Genetics 165:1243-53. 2003..isolation and characterization of orangi (org), an enhancer of shibire (shi), a ts paralytic mutant in Drosophila dynamin. org is an allele of the stress sensitive B (sesB) locus that encodes a mitochondrial adenine nucleotide ..
- Morphogen diffusion: the case of the wingless proteinI The
Nat Cell Biol 2:E79-82. 2000
- Endocytosis: How dynamin sets vesicles PHree!M J Bottomley
Structural Biology Programme, EMBL, Meyerhofstrasse 1, Heidelberg 69117, Germany
Curr Biol 9:R301-4. 1999The dynamin GTPase is required for clathrin-dependent, receptor-mediated endocytosis...
- Genetic evidence for an equilibrium between docked and undocked vesiclesJ Li
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, CA 94305 5345, USA
Philos Trans R Soc Lond B Biol Sci 354:299-306. 1999..Before presenting this data and the significance of the finding for the regulation of neurotransmission, we will briefly review the use of Drosophila genetics as a tool for dissecting synaptic transmission...
- Functional diversity in the dynamin familyA M van der Bliek
Dept of Biological Chemistry, UCLA School of Medicine 90095 1737, USA
Trends Cell Biol 9:96-102. 1999The function of the GTPase dynamin has been discussed for several years. It clearly plays a role in vesicle budding, but, despite recent insights, precisely how it functions in this process is still a matter of debate...
- Cysteine string protein is required for calcium secretion coupling of evoked neurotransmission in drosophila but not for vesicle recyclingR Ranjan
Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6074, USA
J Neurosci 18:956-64. 1998..Collectively, our results indicate that the csp mutation affects calcium secretion coupling of evoked exocytosis but not vesicle recycling. This supports the hypothesis that CSP links synaptic vesicles to calcium secretion coupling...
- Genetic dissection of synaptic transmission in DrosophilaM N Wu
Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
Curr Opin Neurobiol 7:624-30. 1997..In addition, characterization of Drosophila endocytotic mutants provides in vivo evidence for the presence of different endocytotic pathways at a single synapse...
- A framework physical map of Drosophila virilis based on P1 clones: applications in genome evolutionJ Vieira
Department of Organismic and Evolutionary Biology, The Biological Laboratories, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
Chromosoma 106:99-107. 1997..virilis X chromosome. However, a block of four functionally unrelated genes, including white, roughest, Notch, and dunce, seems to be conserved between the two species...
- The dynamins: redundant or distinct functions for an expanding family of related GTPases?R Urrutia
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA
Proc Natl Acad Sci U S A 94:377-84. 1997In the 7 years since dynamin was first isolated from bovine brain in search of novel microtubule-based motors, our understanding of this enzyme has expanded significantly...
- The effect of the inactive mutation on longevity, sex, rhythm and resistance to p-cresol in Drosophila melanogasterK M O'Dell
Institute of Genetics, University of Glasgow, Scotland, UK
Heredity (Edinb) 70:393-9. 1993..Some inferences on the possible mode of action of the iav gene product are discussed...
- Redistribution of synaptic vesicles and their proteins in temperature-sensitive shibire(ts1) mutant DrosophilaJ van de Goor
Hormone Research Institute, University of California, San Francisco 94143 0534, USA
Proc Natl Acad Sci U S A 92:5739-43. 1995..The fraction disappears from homogenates of temperature-sensitive (ts) mutant shibire(ts1) (shi(ts1)) flies paralyzed by exposure to non-permissive temperatures, and reappears on return to permissive ..
- Spontaneous release of multiquantal miniature excitatory junction potentials induced by a Drosophila mutantK Ikeda
Division of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, CA 91010
J Physiol 406:215-23. 1988..of Drosophila melanogaster in both wild-type flies and the temperature-sensitive paralytic mutant, shibirets-1 (shi). 2...
- Tumor-suppressor genes of Drosophila melanogasterE Gateff
Institute of Genetics, Johannes Gutenberg University, Mainz, West Germany
Crit Rev Oncog 1:221-45. 1989
- Genetic analysis of the cellularization of the Drosophila embryoF Schweisguth
Institut Jacques Monod, CNRS, Paris, France
Biol Cell 72:15-23. 1991..Among them, the serendipity alpha gene encodes a membrane-associated protein, which is exclusively accumulated during cellularization, and is required for the reorganization of the microfilaments as the onset of cellularization...
- Synchronized endocytosis studied in the oocyte of a temperature-sensitive mutant of Drosophila melanogasterT Tsuruhara
Division of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California 91010
Cell Tissue Res 259:199-207. 1990..The observations suggest that these organelles transform one into another within this sequence...
- Notch signal transduction: a real rip and moreG Weinmaster
Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, 90095 1737, USA
Curr Opin Genet Dev 10:363-9. 2000..Some recent findings support a model in which sequential proteolytic cleavage serves to regulate Notch signal transduction...
- Endocytosis: why not wait to deubiquitinate?R W Carthew
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
Curr Biol 10:R532-4. 2000..Surprisingly, this is the opposite effect that ubiquitination has on endocytosis of many plasma membrane proteins...
- Distinct requirements for the AP-3 adaptor complex in pigment granule and synaptic vesicle biogenesis in Drosophila melanogasterC Mullins
Cell Biology and Metabolism Branch, National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Mol Gen Genet 263:1003-14. 2000..Together, these results demonstrate that Drosophila AP-3 is critical for the biogenesis of pigment granules, but is apparently not essential for formation of a major population of synaptic vesicles in vivo...
- Trafficking through Rab11 endosomes is required for cellularization during Drosophila embryogenesisAnne Pelissier
Laboratoire de Genetique et de Physiologie du Developpement, Institut de Biologie du Developpement de Marseille, France
Curr Biol 13:1848-57. 2003..In Drosophila, the process is specialized and called cellularization. The trafficking pathways that underlie this process and that are responsible for the mobilization of membrane pools, however, remain poorly understood...
- Targeted expression of shibire ts and semaphorin 1a reveals critical periods for synapse formation in the giant fiber of DrosophilaR K Murphey
Department of Biology, Morrill Science Center, University of Massachusetts, Amherst, MA 01003, USA
Development 130:3671-82. 2003..Temporal control of targeted expression of the semaphorin 1a gene showed that acute excess Semaphorin 1a had a permanent disruptive effect on synapse formation...
- Mushroom body memoir: from maps to modelsMartin Heisenberg
, Am Hubland, , Germany
Nat Rev Neurosci 4:266-75. 2003
- Modulating sphingolipid biosynthetic pathway rescues photoreceptor degenerationUsha Acharya
Regulation of Cell Growth Laboratory, National Cancer Institute Frederick, Frederick, MD 21702, USA
Science 299:1740-3. 2003..Both genetic backgrounds modulated the endocytic machinery because they suppressed defects in a dynamin mutant...
- Do morphogen gradients arise by diffusion?Arthur D Lander
Department of Developmental and Cell Biology, Developmental Biology Center, University of California, Irvine 92697, USA
Dev Cell 2:785-96. 2002..Moreover, we show that a class of experiments, endocytic blockade, thought to effectively distinguish between diffusive and nondiffusive transport models actually fails to draw useful distinctions...
- Expression and changing distribution of the human transferrin receptor in developing Drosophila oocytes and embryosM S Bretscher
Division of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge, UK
J Cell Sci 109:3113-9. 1996....
- Neuralized: regulating notch by putting away deltaH Kramer
Center for Basic Neuroscience, UT Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
Dev Cell 1:725-6. 2001..Neuralized acts in a subset of Notch-dependent cell fate decisions including lateral inhibition in Drosophila neurogenesis. Three recent papers reveal that Neuralized acts as a ubiquitin ligase and triggers endocytosis of the ligand Delta...
- MOLECULAR BIOLOGY OF DROSOPHILA LEARNING AND MEMORYRonald Davis; Fiscal Year: 2000....
- Drosophila as an Experimental Model for EpilepsyJ Troy Littleton; Fiscal Year: 2010..We propose to use Drosophila as a model system to explore the cellular and molecular mechanisms by which enhanced neuronal activity couples to modifications of synaptic connectivity during epilespy. ..
- INTEGRIN FUNCTION IN MEMORYRonald Davis; Fiscal Year: 2009..Insights for the improvement of the diagnosis and treatment of diseases of working memory may be gathered from these studies. ..
- Olfactory memory trace formation in DrosophilaRonald Davis; Fiscal Year: 2009..This will aid in our understanding of the diseases that affect memory formation. ..
- Systems Biology of Morphogenesis and Spatial Information FlowARTHUR LANDER; Fiscal Year: 2007..Such insights will have a broad impact on a variety of health-related areas including human development and birth defects, stem cells and regeneration, normal and tumor cell growth, and basic physiology. ..
- MOLECULAR BIOLOGY OF DROSOPHILA LEARNING AND MEMORYRonald Davis; Fiscal Year: 2009....
- INTEGRIN FUNCTION IN MEMORYRonald Davis; Fiscal Year: 2003..In addition, the research may highlight possible points of molecular dysfunction underlying cognitive diseases. ..
- MOLECULAR BIOLOGY OF DROSOPHILA LEARNING AND MEMORYRonald Davis; Fiscal Year: 2005..This basic information is expected to be of general significance to learning in other species and of relevance to diseases that affect cognition. ..
- Neuroadaptations to Ethanol in DrosophilaTherese Kosten; Fiscal Year: 2007..These studies will further our understanding of the genetic basis for ethanol sensitivity and tolerance, important parameters for susceptibility to alcoholism. ..
- Generation and validation of inducible Cre driver lines by enhancer trappingRonald Davis; Fiscal Year: 2007..This is the lesson learned from Drosophila genetics and there is every reason to expect it to hold in the mouse. ..
- Cyclic AMP Signaling and Bipolar DisorderRonald Davis; Fiscal Year: 2007..Public Health Relevance: Bipolar Disorder is a prevalent brain disorder. The studies proposed here will help to elucidate the genetic basis for the disease. ..
- Olfactory memory trace formation in DrosophilaRonald Davis; Fiscal Year: 2007..These experiments will significantly increase our understanding of how memories form and the events that can potentially perturb normal memory formation. This will aid in our understanding of the diseases that affect memory formation. ..
- INTEGRIN FUNCTION IN MEMORYRonald Davis; Fiscal Year: 2007..Insights for the improvement of the diagnosis and treatment of diseases of working memory may be gathered from these studies. ..
- NEUROBIOLOGICALLY IMPORTANT GENES IIRonald Davis; Fiscal Year: 1991....
- Regulated Gene Expression and Drosophila BehaviorRonald Davis; Fiscal Year: 2003..The techniques proposed here offer a general method for turning on gene activity both spatially and temporally in Drosophila. ..
- Endocytic Control of Notch-Mediated Cell Fate Decisions in NeurogenesisFABRICE J ROEGIERS; Fiscal Year: 2010....
- Motivational control of appetitive memoryScott Waddell; Fiscal Year: 2009....
- TRANSCRIPTIONAL DETERMINANTS OF B LYMPHOCYTESTHOMAS KADESCH; Fiscal Year: 2002....
- Transport and Complexity in Biological SystemsARTHUR LANDER; Fiscal Year: 2004..abstract_text> ..
- Circadian control of a behavioral outputJohn Ewer; Fiscal Year: 2007..Thus it offers a unique opportunity to elucidate how the activity of the clock is coupled to the mechanism that controls a behavioral output. ..
- Functional Anatomy of Learning and Memory in DrosophilaJOSHUA DUBNAU; Fiscal Year: 2007..to this proposal is the availability in flies of a Gal4-responsive temperature-sensitive (and dominant negative) dynamin transgene (UAS-Shi/ ts), which permits temperature dependent and reversible disruption of neuronal function...