Genomes and Genes
Gene Symbol: shi
Alias: CG18102, DNM3, DYN, Ddyn, Ddyn3, Ddyn4, Dmel\CG18102, Dyn, Shi, anon-WO0153538.12, anon-WO0153538.13, anon-WO0153538.14, dDyn, dyn, l(1)VII, l(1)shi, shibere, CG18102-PA, CG18102-PB, CG18102-PC, CG18102-PE, CG18102-PF, CG18102-PG, CG18102-PH, CG18102-PI, CG18102-PJ, CG18102-PK, CG18102-PL, CG18102-PM, CG18102-PN, CG18102-PO, dynamin, shi-PA, shi-PB, shi-PC, shi-PE, shi-PF, shi-PG, shi-PH, shi-PI, shi-PJ, shi-PK, shi-PL, shi-PM, shi-PN, shi-PO, shibere, shibiri
Species: fruit fly
Publications140 found, 100 shown here
- Rolling blackout is required for synaptic vesicle exocytosisFu De Huang
Department of Biological Sciences, Kennedy Center for Research on Human Development, Brain Institute, Vanderbilt University, Nashville, Tennessee 37235 1634, USA
J Neurosci 26:2369-79. 2006..We conclude that RBO plays an essential role in neurotransmitter release, downstream of SV docking, likely mediating SV fusion...
- Dynamin self-assembles into rings suggesting a mechanism for coated vesicle buddingJ E Hinshaw
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037
Nature 374:190-2. 1995b>DYNAMIN, a 100K member of the GTPase superfamily, is the mammalian homologue of the Drosophila shibire gene product...
- Drosophila Cip4/Toca-1 integrates membrane trafficking and actin dynamics through WASP and SCAR/WAVERobert Fricke
Institut fur Neurobiologie, Wilhelms University Münster, 48149 Munster, Germany
Curr Biol 19:1429-37. 2009..The F-BAR-domain-containing proteins are prime candidates to couple actin dynamics and membrane trafficking in different morphogenetic processes...
- Mushroom body efferent neurons responsible for aversive olfactory memory retrieval in DrosophilaJulien Séjourné
Genes and Dynamics of Memory Systems, Neurobiology Unit, CNRS, Ecole Superieure de Physique et de Chimie Industrielles, Paris, France
Nat Neurosci 14:903-10. 2011..As the lateral horn has been implicated in innate responses to repellent odorants, we propose that MB-V2 neurons recruit the olfactory pathway involved in innate odor avoidance during memory retrieval...
- A pair of inhibitory neurons are required to sustain labile memory in the Drosophila mushroom bodyJena L Pitman
Department of Neurobiology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
Curr Biol 21:855-61. 2011..We propose that APL neurons provide widespread inhibition to stabilize and maintain synaptic specificity of a labile memory trace in a recurrent DPM and MB α'β' neuron circuit...
- A sensory feedback circuit coordinates muscle activity in DrosophilaCynthia L Hughes
Molecular Neurobiology Laboratory, The Salk Institute, 10010 N Torrey Pines Rd, La Jolla, CA 92037, USA
Mol Cell Neurosci 35:383-96. 2007....
- Conditional modification of behavior in Drosophila by targeted expression of a temperature-sensitive shibire allele in defined neuronsT Kitamoto
Division of Neurosciences, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, California 91010, USA
J Neurobiol 47:81-92. 2001..In this method, a temperature-sensitive allele of shibire (shi(ts1)) is overexpressed in neuronal subsets using the GAL4/UAS system...
- Discrete residues in the c(2)b domain of synaptotagmin I independently specify endocytic rate and synaptic vesicle sizeKira E Poskanzer
Department of Biochemistry and Biophysics, University of California, San Francisco, Rock Hall, 1550 Fourth Street, Box 2822, San Francisco, California 94158, USA
Neuron 50:49-62. 2006....
- The Drosophila epsin 1 is required for ubiquitin-dependent synaptic growth and function but not for synaptic vesicle recyclingHong Bao
Department of Zoology, University of Oklahoma, Norman, OK 73019, USA
Traffic 9:2190-205. 2008..These observations demonstrate that Lqf plays distinct roles in two ubiquitin pathways to regulate structural and functional plasticity of the synapse...
- Patched controls the Hedgehog gradient by endocytosis in a dynamin-dependent manner, but this internalization does not play a major role in signal transductionCarlos Torroja
Centro de Biologia Molecular Severo Ochoa, CSIC, Universidad Autonoma de Madrid, Cantoblanco, E 28049 Madrid, Spain
Development 131:2395-408. 2004..Our studies show that Ptc limits the Hh gradient by internalizing Hh through endosomes in a dynamin-dependent manner, and that both Hh and Ptc are targeted to lysosomal degradation...
- Light-dependent subcellular translocation of Gqalpha in Drosophila photoreceptors is facilitated by the photoreceptor-specific myosin III NINACMichelle A Cronin
Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
J Cell Sci 117:4797-806. 2004....
- Subcellular localization of the segment polarity protein patched suggests an interaction with the wingless reception complex in Drosophila embryosJ Capdevila
Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, Spain
Development 120:987-98. 1994..These data suggest the interaction of patched protein with elements of the reception complex of wingless, as a way to control the wingless expression...
- Temperature-sensitive mutations in Drosophila melanogaster. XIV. A selection of immobile adultsT A Grigliatti
Mol Gen Genet 120:107-14. 1973
- Dopamine-mushroom body circuit regulates saliency-based decision-making in DrosophilaKe Zhang
Institute of Neuroscience, Key Laboratory of Neurobiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences CAS, 320 Yueyang Road, Shanghai 200031, China
Science 316:1901-4. 2007..Immunohistological analysis showed that mushroom bodies are densely innervated by dopaminergic axons. Thus, the circuit from the dopamine system to mushroom bodies is crucial for choice behavior in Drosophila...
- Amphiphysin is necessary for organization of the excitation-contraction coupling machinery of muscles, but not for synaptic vesicle endocytosis in DrosophilaA Razzaq
Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK
Genes Dev 15:2967-79. 2001Amphiphysins 1 and 2 are enriched in the mammalian brain and are proposed to recruit dynamin to sites of endocytosis. Shorter amphiphysin 2 splice variants are also found ubiquitously, with an enrichment in skeletal muscle...
- Sequential use of mushroom body neuron subsets during drosophila odor memory processingMichael J Krashes
Department of Neurobiology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
Neuron 53:103-15. 2007....
- Myosin VI is required for E-cadherin-mediated border cell migrationErika R Geisbrecht
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 2185, USA
Nat Cell Biol 4:616-20. 2002..Multiple biochemical mechanisms may exist, therefore, for cadherins to associate with diverse unconventional myosins that are required for normal stereocilium formation or maintenance...
- Role of Drosophila Rab5 during endosomal trafficking at the synapse and evoked neurotransmitter releaseTanja Wucherpfennig
Max Planck Institut für molekulare Zellbiologie und Genetik, Dresden, Germany
J Cell Biol 161:609-24. 2003..vesicles pool is depleted, the endosome is also drastically reduced and only recovers from membrane derived by dynamin-mediated endocytosis...
- Mushroom bodies modulate salience-based selective fixation behavior in DrosophilaWang Xi
State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China
Eur J Neurosci 27:1441-51. 2008....
- Mushroom body memoir: from maps to modelsMartin Heisenberg
Biozentrum der Universität Würzburg, Am Hubland, D 97074 Wurzburg, Germany
Nat Rev Neurosci 4:266-75. 2003
- Dap160/intersectin scaffolds the periactive zone to achieve high-fidelity endocytosis and normal synaptic growthBruno Marie
Department of Biochemistry and Biophysics, 1550 4th Street, GDBS Fourth Floor North, University of California, San Francisco, 94143, USA
Neuron 43:207-19. 2004..dap160 mutant synapses have decreased levels of essential endocytic proteins, including dynamin, endophilin, synaptojanin, and AP180, while other markers of the active zone and periactive zone are generally ..
- A molecular pathway for light-dependent photoreceptor apoptosis in DrosophilaA Kiselev
Department of Pharmacology, UT Southwestern Medical Center, Dallas, Texas 75390, USA
Neuron 28:139-52. 2000..Together, these data define the proapoptotic molecules in Drosophila photoreceptors and indicate a novel signaling pathway for light-activated rhodopsin molecules in control of photoreceptor viability...
- Slow oscillations in two pairs of dopaminergic neurons gate long-term memory formation in DrosophilaPierre Yves Plaçais
Genes and Dynamics of Memory Systems, Neurobiology Unit, Centre National de Recherche Scientifique, Ecole Superieure de Physique et de Chimie Industrielles, Paris, France
Nat Neurosci 15:592-9. 2012..Oscillations were weakened by ARM-inducing massed training and were enhanced during LTM formation. Our results indicate that oscillations of two pairs of dopaminergic neurons control ARM levels and gate LTM...
- Synchronized endocytosis studied in the oocyte of a temperature-sensitive mutant of Drosophila melanogasterT Tsuruhara
Division of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California 91010
Cell Tissue Res 259:199-207. 1990..The observations suggest that these organelles transform one into another within this sequence...
- NSF function in neurotransmitter release involves rearrangement of the SNARE complex downstream of synaptic vesicle dockingL A Tolar
Department of Genetics, University of Washington, Seattle, Washington 98195, USA
J Neurosci 18:10250-6. 1998....
- Dopamine and octopamine differentiate between aversive and appetitive olfactory memories in DrosophilaMartin Schwaerzel
Lehrstuhl für Genetik und Neurobiologie, Biozentrum, Am Hubland, D 97074 Wurzburg, Germany
J Neurosci 23:10495-502. 2003..Our results suggest that in associative conditioning, different memories are formed of the same odor under different circumstances, and that they are linked to the respective motivational systems by their specific modulatory pathways...
- Exclusive consolidated memory phases in DrosophilaGuillaume Isabel
Developpement, Evolution, Plasticité du Système Nerveux, CNRS, 1 Avenue de la Terrasse, 91190 Gifsur Yvette, France
Science 304:1024-7. 2004..We propose that ARM acts as a gating mechanism that ensures that LTM is formed only after repetitive and spaced training...
- Hexagonal packing of Drosophila wing epithelial cells by the planar cell polarity pathwayAnne Kathrin Classen
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01309 Dresden, Germany
Dev Cell 9:805-17. 2005..We propose that these proteins polarize trafficking of Cadherin-containing exocyst vesicles during junction remodeling. This may be a common mechanism for the action of planar cell polarity proteins in diverse systems...
- Dynein light chain 1 regulates dynamin-mediated F-actin assembly during sperm individualization in DrosophilaAnindya Ghosh-Roy
Tata Institute of Fundamental Research, Mumbai 400 005, India
Mol Biol Cell 16:3107-16. 2005..In addition, DDLC1 colocalizes with dynamin along investment cones and regulates F-actin assembly at this organelle by retaining dynamin along the cones...
- The formation of stable rhodopsin-arrestin complexes induces apoptosis and photoreceptor cell degenerationP G Alloway
Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
Neuron 28:129-38. 2000..We propose that an identical mechanism may be responsible for the pathology found in a subset of human retinal degenerative disorders...
- Male-specific fruitless specifies the neural substrates of Drosophila courtship behaviourDevanand S Manoli
Neurosciences Program, Stanford University, Stanford, California 94305, USA
Nature 436:395-400. 2005..Together, these data demonstrate that FruM proteins specify the neural substrates of male courtship...
- The amnesiac gene product is expressed in two neurons in the Drosophila brain that are critical for memoryS Waddell
Center for Learning and Memory Department of Brain and Cognitive Sciences Department of Biology Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Cell 103:805-13. 2000..These results indicate that AMN neuropeptide release onto the mushroom bodies is critical for normal olfactory memory...
- Wingless gradient formation in the Drosophila wingM Strigini
European Molecular Biology Laboratory, Heidelberg, 69117, Germany
Curr Biol 10:293-300. 2000..Wg protein is found in vesicles in Wg-responsive cells in the embryo and imaginal discs. It has been proposed that Wg may be transported by a vesicle-mediated mechanism...
- A neural circuit mechanism integrating motivational state with memory expression in DrosophilaMichael J Krashes
Department of Neurobiology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
Cell 139:416-27. 2009..Therefore, dNPF and dopamine provide a motivational switch in the mushroom body that controls the output of appetitive memory...
- Dap160, a neural-specific Eps15 homology and multiple SH3 domain-containing protein that interacts with Drosophila dynaminJ Roos
Department of Biochemistry and Biophysics and the Hormone Research Institute, University of California, San Francisco, California 94143 0534, USA
J Biol Chem 273:19108-19. 1998The discovery of overlapping hot spots of dynamin (Estes, P. S., Roos, J., van der Bliek, A., Kelly, R. B., Krishnan, K. S., and Ramaswami, M. (1996) J. Neurosci...
- G-protein gamma subunit 1 is required for sugar reception in DrosophilaHiroshi Ishimoto
Department of Biology, Graduate School of Sciences, Kyushu University, Ropponmatsu, Fukuoka, Japan
EMBO J 24:3259-65. 2005..neural modulator genes such as the modified Shaker K+ channel (EKO), the tetanus toxin light chain or the shibire (shi(ts1)) gene...
- Salience modulates 20-30 Hz brain activity in DrosophilaBruno van Swinderen
The Neurosciences Institute, 10640 John Jay Hopkins Drive, San Diego, California 92121, USA
Nat Neurosci 6:579-86. 2003..We demonstrated the association of these responses with behavioral tracking and initiated a genetic approach to investigating neural correlates of perception...
- Nucleoside diphosphate kinase, a source of GTP, is required for dynamin-dependent synaptic vesicle recyclingK S Krishnan
Department of Biological Sciences, Tata Institute for Fundamental Research, Homi Bhabha Road, 400005, Colaba, Bombay, India
Neuron 30:197-210. 2001..We demonstrate that NDK regulates synaptic vesicle internalization at the stage where function of the dynamin GTPase is required...
- Distinctive neuronal networks and biochemical pathways for appetitive and aversive memory in Drosophila larvaeKen Honjo
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan
J Neurosci 29:852-62. 2009..These results as a whole suggest that the genetically programmed memory circuitries might provide predisposition in the efficacy of inducing longer-lived memory components in associative learning...
- Cdc42, Par6, and aPKC regulate Arp2/3-mediated endocytosis to control local adherens junction stabilityMarios Georgiou
MRC Laboratory for Molecular Cell Biology, Division of Life Sciences, University College London, Gower Street, London WC1E 6BT, UK
Curr Biol 18:1631-8. 2008....
- Peripheral multidendritic sensory neurons are necessary for rhythmic locomotion behavior in Drosophila larvaeWei Song
Department of Physiology, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA
Proc Natl Acad Sci U S A 104:5199-204. 2007..These findings reveal that MD sensory neuron input is a necessary component in the neural circuitry that generates larval locomotion...
- Increased dopaminergic signaling impairs aversive olfactory memory retention in DrosophilaShixing Zhang
State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China
Biochem Biophys Res Commun 370:82-6. 2008..Taken together, our findings suggest that increased dopaminergic signaling impairs aversive olfactory memory retention in flies...
- Modulation of intracellular trafficking regulates cell intercalation in the Drosophila tracheaDaniel D Shaye
Institut de Biologia Molecular de Barcelona CSIC, C Baldiri Reixac 10, 08028 Barcelona, Spain
Nat Cell Biol 10:964-70. 2008..Our work suggests a mechanism by which trafficking of adhesion molecules regulates intercalation, and shows how this mechanism can be modulated in vivo to influence cell behaviour...
- Drosophila homologue of Eps15 is essential for synaptic vesicle recyclingAmitabha Majumdar
Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba Mumbai 400005, India
Exp Cell Res 312:2288-98. 2006..Eps15 is enriched in the larval neuromuscular junction in endocytic 'hot spots' in a pattern similar to Dynamin. Eps15 mutants show a decrease in the alpha-Adaptin levels at the larval neuromuscular junction synapse...
- Extinction antagonizes olfactory memory at the subcellular levelMartin Schwaerzel
Theodor Boveri Institut für Biowissenschaften, Lehrstuhl für Genetik und Neurobiologie, Biozentrum, Am Hubland, Wurzburg, Germany
Neuron 35:951-60. 2002..Thus, unreinforced presentation of learned odorants antagonizes intracellularly the signaling cascade underlying memory formation...
- Synaptic physiology and ultrastructure in comatose mutants define an in vivo role for NSF in neurotransmitter releaseF Kawasaki
Department of Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
J Neurosci 18:10241-9. 1998..Together, the results reported here define a role for dNSF1 in the priming of docked synaptic vesicles for calcium-triggered fusion...
- Drosophila DPM neurons form a delayed and branch-specific memory trace after olfactory classical conditioningDinghui Yu
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
Cell 123:945-57. 2005..DPM neurons are therefore "odor generalists" and form a delayed, branch-specific, and amnesiac-dependent memory trace that may guide behavior after acquisition...
- Role of Drosophila alpha-adaptin in presynaptic vesicle recyclingM Gonzalez-Gaitan
Max Planck Institut fur biophysikalische Chemie, Abt Molekulare Entwicklungsbiologie, Gottingen, Germany
Cell 88:767-76. 1997..In presynaptic terminals, alpha-adaptin defines a network-like membrane structure to which the GTPase dynamin is recruited...
- Blockade of neurotransmission in Drosophila mushroom bodies impairs odor attraction, but not repulsionYalin Wang
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
Curr Biol 13:1900-4. 2003....
- A role for myosin VI in actin dynamics at sites of membrane remodeling during Drosophila spermatogenesisAaron D Rogat
Department of Biology, Washington University, One Brookings Drive, St Louis, MO 63130, USA
J Cell Sci 115:4855-65. 2002..In addition, we show that dynamin localizes to these actin structures and that when dynamin and myosin VI function are both impaired, major defects ..
- Shibire mutations reveal distinct dynamin-independent and -dependent endocytic pathways in primary cultures of Drosophila hemocytesA Guha
National Center for Biological Sciences, Tata Institute of Fundamental Research, GKVK, Bangalore 560 065, India
J Cell Sci 116:3373-86. 2003..In primary culture, the endocytic phenotype of ALBR internalization in cells carrying mutations in Drosophila Dynamin (dDyn) at the shibire locus (shits) parallels the temperature-sensitive behavior of shits animals...
- Electrophysiological correlates of rest and activity in Drosophila melanogasterDouglas A Nitz
The Neurosciences Institute, 10640 John Jay Hopkins Drive, San Diego, CA 92121, USA
Curr Biol 12:1934-40. 2002....
- Neural substrates of memory: from synapse to systemJosh Dubnau
Cold Spring Harbor Laboratories, 1 Bungtown Rd, Cold Spring Harbor, New York 11724, USA
J Neurobiol 54:238-53. 2003..Our objective here is to offer a conceptually unifying perspective and to discuss this perspective in relation to an experiment analysis of memory in Drosophila...
- A role for actin dynamics in individualization during spermatogenesis in Drosophila melanogasterTatsuhiko Noguchi
Department of Biology, Washington University in St Louis, Campus Box 1229, 1 Brookings Drive, St Louis, MO 63130, USA
Development 130:1805-16. 2003..We also suggest that endocytosis and exocytosis might not be important for membrane remodeling. This system should be suitable for analysis of defects in male sterile mutants and for investigating other steps of spermatogenesis...
- The neural substrate of spectral preference in DrosophilaShuying Gao
Unit on Neuronal Connectivity, Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
Neuron 60:328-42. 2008..We propose that Dm8 sacrifices spatial resolution for sensitivity by relaying signals from multiple R7s to projection neurons, which then provide output to higher visual centers...
- Endophilin promotes a late step in endocytosis at glial invaginations in Drosophila photoreceptor terminalsRuth Fabian-Fine
Neuroscience Institute, Department of Psychology, Life Sciences Centre, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4J1
J Neurosci 23:10732-44. 2003..Using dynamin;endo double-mutant photoreceptors, we provide further evidence that ultimately the function of Endophilin is ..
- Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivoKira E Poskanzer
Department of Biochemistry and Biophysics, Program in Neuroscience, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, California 94143, USA
Nature 426:559-63. 2003..By inactivating Syt I only during endocytosis, we demonstrate that Syt I is necessary for the endocytosis of synaptic vesicles that have undergone exocytosis using a functional Syt I protein...
- Endocytic trafficking of Wingless and its receptors, Arrow and DFrizzled-2, in the Drosophila wingAnna F Rives
University of Pennsylvania School of Medicine, Department of Cell and Developmental Biology, Philadelphia, PA 190104 6058, USA
Dev Biol 293:268-83. 2006..Thus, signal modulation by endocytic routing will depend on the tissue to be patterned and the goals during that patterning event...
- Functional analysis of fruitless gene expression by transgenic manipulations of Drosophila courtshipAdriana Villella
Department of Biology, Brandeis University, Waltham, MA 02454, USA
Proc Natl Acad Sci U S A 102:16550-7. 2005..males toward females and courtship among males when driving a conditional disrupter of synaptic transmission (shi(TS))...
- Bruchpilot, a protein with homology to ELKS/CAST, is required for structural integrity and function of synaptic active zones in DrosophilaDhananjay A Wagh
Lehrstuhl für Genetik und Neurobiologie, Theodor Boveri Institut für Biowissenschaften, Am Hubland, D 97074 Wurzburg, Germany
Neuron 49:833-44. 2006..We propose that BRP is critical for intact AZ structure and normal-evoked neurotransmitter release at chemical synapses of Drosophila...
- Synaptic mitochondria are critical for mobilization of reserve pool vesicles at Drosophila neuromuscular junctionsPatrik Verstreken
Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
Neuron 47:365-78. 2005In a forward screen for genes affecting neurotransmission in Drosophila, we identified mutations in dynamin-related protein (drp1)...
- Dopaminergic modulation of arousal in DrosophilaRozi Andretic
The Neuroscience Institute, San Diego, California 92121, USA
Curr Biol 15:1165-75. 2005..Drosophila behaviors have been studied that span different levels of arousal, from sleep to visual perception to psychostimulant responses...
- Internalization is required for proper Wingless signaling in Drosophila melanogasterElaine S Seto
Department of Molecular and Human Genetics, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA
J Cell Biol 173:95-106. 2006..In Drosophila melanogaster cells stimulated with Wg media, dynamin or Rab5 knockdown causes reduced Super8XTOPflash activity, suggesting that internalization and endosomal ..
- Synapse scaffolding: intersection of endocytosis and growthKendal Broadie
Department of Biological Sciences, Kennedy Center for Research on Human Development, Vanderbilt University, Nashville, Tennessee 37232, USA
Curr Biol 14:R853-5. 2004The Drosophila dynamin-associated protein Dap160, homolog of the vertebrate Intersectins, is thought likely to act as a molecular scaffold in the synaptic periactive zone...
- Arrow (LRP6) and Frizzled2 cooperate to degrade Wingless in Drosophila imaginal discsEugenia Piddini
National Institute for Medical Research, The Ridgeway Mill Hill, London NW7 1AA, UK
Development 132:5479-89. 2005..Overall, Frizzled2 achieves ligand capture and internalization, whereas Arrow, and perhaps downstream signalling, are essential for lysosomal targeting...
- Differential potencies of effector genes in adult DrosophilaAndreas S Thum
Lehrstuhl Genetik und Neurobiologie, Universität Würzburg Am Hubland Biozentrum, D 97074 Wurzburg, Germany
J Comp Neurol 498:194-203. 2006..Altogether, the efficiency of effector genes depends on properties of the targeted neurons. Thus, we conclude that the selection of the appropriate effector gene is critical for evaluating the function of neural circuits...
- Four-dimensional gene expression control: memories on the flyBenjamin Leung
Department of Neurobiology, University of Massachusetts Medical School, Worcester MA 01605, USA
Trends Neurosci 27:511-3. 2004..Two recent papers describe implementation of different but related technologies that now provide this missing element in fly behavioral research...
- Dissection of the peripheral motion channel in the visual system of Drosophila melanogasterJens Rister
Lehrstuhl für Genetik und Neurobiologie, Universitat Wurzburg, Am Hubland, 97074, Wurzburg, Germany
Neuron 56:155-70. 2007..At low contrast, L1 and L2 depend upon each other for motion processing. Of the two minor pathways, amc/T1 specifically enhances the L1 pathway at intermediate contrast. L3 appears not to contribute to motion but to orientation behavior...
- The endocytic pathway and formation of the Wingless morphogen gradientEric Marois
Max Planck Institute for Cell Biology and Genetics, Pfotenhauerstr 108, 01307 Dresden, Germany
Development 133:307-17. 2006..The glypican Dally-like promotes Wingless spreading by unknown mechanisms, while Dynamin-dependent endocytosis is thought to restrict Wingless spread...
- The J-domain protein Rme-8 interacts with Hsc70 to control clathrin-dependent endocytosis in DrosophilaHenry C Chang
Dept of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, PO Box 208002, New Haven, CT 06520 8002, USA
J Cell Biol 164:1055-64. 2004By screening for mutants exhibiting interactions with a dominant-negative dynamin, we have identified the Drosophila homologue of receptor-mediated endocytosis (Rme) 8, a J-domain-containing protein previously shown to be required for ..
- Unique biochemical and behavioral alterations in Drosophila shibire(ts1) mutants imply a conformational state affecting dynamin subcellular distribution and synaptic vesicle cyclingMai Lei Chen
Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA
J Neurobiol 53:319-29. 2002b>Dynamin is a GTPase protein that is essential for clathrin-mediated endocytosis of synaptic vesicle membranes. The Drosophila dynamin mutation shi(ts1) changes a single residue (G273D) at the boundary of the GTPase domain...
- Conditional disruption of synaptic transmission induces male-male courtship behavior in DrosophilaToshihiro Kitamoto
Division of Neurosciences, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA 91010, USA
Proc Natl Acad Sci U S A 99:13232-7. 2002..A temperature-sensitive allele of the Drosophila dynamin gene shibire (shi(ts1)) was expressed by using the GAL4/UAS system to disrupt synaptic transmission from GAL4-..
- Drosophila stoned proteins regulate the rate and fidelity of synaptic vesicle internalizationD T Stimson
Department of Molecular and Cellular Biology and Arizona Research Laboratories Division of Neurobiology, University of Arizona, Tucson, Arizona 85721, USA
J Neurosci 21:3034-44. 2001At an initial step during synaptic vesicle recycling, dynamin and adaptor proteins mediate the endocytosis of synaptic vesicle components from the plasma membrane...
- Traffic of dynamin within individual Drosophila synaptic boutons relative to compartment-specific markersP S Estes
Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721, USA
J Neurosci 16:5443-56. 1996..trap an intermediate stage in synaptic vesicle recycling, we have examined the localization and redistribution of dynamin within single synaptic varicosities at the larval neuromuscular junction...
- The role of Drosophila mushroom body signaling in olfactory memoryS E McGuire
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
Science 293:1330-3. 2001..neurotransmission through this region of the brain by expressing a temperature-sensitive allele of the shibire dynamin guanosine triphosphatase, which is required for synaptic transmission...
- Disruption of neurotransmission in Drosophila mushroom body blocks retrieval but not acquisition of memoryJ Dubnau
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
Nature 411:476-80. 2001..We have used a temperature-sensitive dynamin transgene, which disrupts synaptic transmission reversibly and on the time-scale of minutes, to investigate the ..
- Genetic dissection of endocytic trafficking in Drosophila using a horseradish peroxidase-bride of sevenless chimera: hook is required for normal maturation of multivesicular endosomesA Sunio
Center for Basic Neuroscience, Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9111, USA
Mol Biol Cell 10:847-59. 1999..Our genetic analysis indicates that Hook is required late in endocytic trafficking to negatively regulate delivery from mature MVBs to multilammelar late endosomes and lysosomes...
- Size of vesicle pools, rates of mobilization, and recycling at neuromuscular synapses of a Drosophila mutant, shibireR Delgado
Centro de Estudios Cientificos, Arturo Prat 514, Valdivia, Chile
Neuron 28:941-53. 2000..Vesicle recycling supports synaptic transmission during prolonged tetanic stimulation and the maximum recycling rate was 1000 vesicles/s...
- Interaction of stoned and synaptotagmin in synaptic vesicle endocytosisT Fergestad
Department of Biology, University of Utah, Salt Lake City, Utah 84112 0840, USA
J Neurosci 21:1218-27. 2001..Both Stoned proteins colocalize precisely with endocytotic proteins including the AP2 complex and Dynamin in the "lattice network" characteristic of endocytotic domains in Drosophila presynaptic terminals...
- Lava lamp, a novel peripheral golgi protein, is required for Drosophila melanogaster cellularizationJ C Sisson
Department of Molecular, Cell and Developmental Biology, Sinsheimer Labs, University of California at Santa Cruz, Santa Cruz, California 95064, USA
J Cell Biol 151:905-18. 2000..have been previously implicated in cellularization and/or cytokinesis, including KLP3A, Anillin, Septins, and Dynamin. We now show that a novel MMAP, Lava Lamp (Lva), is also required for cellularization...
- Requirements for presenilin-dependent cleavage of notch and other transmembrane proteinsG Struhl
Department of Genetics and Development, Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA
Mol Cell 6:625-36. 2000..Hence, Notch, beta-APP, and perhaps other proteins may be targeted for Presenilin-mediated transmembrane cleavage by upstream processing events that sever the extracellular domain from the rest of the protein...
- Synaptic vesicle size and number are regulated by a clathrin adaptor protein required for endocytosisB Zhang
Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
Neuron 21:1465-75. 1998..These results provide novel insights into the molecular mechanism of endocytosis and reveal a role for AP180 in regulating vesicle size through a clathrin-dependent reassembly process...
- Temperature-sensitive paralytic mutations demonstrate that synaptic exocytosis requires SNARE complex assembly and disassemblyJ T Littleton
Laboratory of Genetics, University of Wisconsin, Madison 53706, USA
Neuron 21:401-13. 1998..These results provide in vivo evidence that cycles of assembly and disassembly of SNARE complexes drive membrane trafficking at synapses...
- Two distinct pools of synaptic vesicles in single presynaptic boutons in a temperature-sensitive Drosophila mutant, shibireH Kuromi
Institute for Behavioral Sciences, Gunma University School of Medicine, Maebashi, Japan
Neuron 20:917-25. 1998..Cytochalasin D treatment eliminated the reserve pool and reduced synaptic transmission evoked by high frequency stimulation. Thus, the reserve pool may play a crucial role for sustaining high frequency synaptic transmission...
- Directionality of wingless protein transport influences epidermal patterning in the Drosophila embryoM M Moline
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208 3500, USA
Development 126:4375-84. 1999..We have constructed a transgene expressing a dominant negative form of shibire (shi), the fly dynamin homologue...
- Hsc70 is required for endocytosis and clathrin function in DrosophilaHenry C Chang
Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520 8002, USA
J Cell Biol 159:477-87. 2002..The Hsc70-4 mutation also interacted genetically with a dominant-negative mutant of dynamin, a gene required for the budding of clathrin-coated vesicles (CCVs)...
- Requirement for dynamin during Notch signaling in Drosophila neurogenesisL Seugnet
Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, Illkirch, France
Dev Biol 192:585-98. 1997..The loss of activity of dynamin, which is encoded by the gene shibire and is required for endocytosis, results in a similar phenotype...
- Endophilin is critically required for synapse formation and function in Drosophila melanogasterRicha Rikhy
Tata Institute of Fundamental Research, Mumbai 400 005 India
J Neurosci 22:7478-84. 2002..Immunofluorescence studies show colocalization of endophilin with dynamin consistent with a possible role in synaptic vesicle recycling.
- Endophilin mutations block clathrin-mediated endocytosis but not neurotransmitter releasePatrik Verstreken
Program in Developmental Biology, Houston, TX, USA
Cell 109:101-12. 2002..We propose that kiss-and-run maintains neurotransmission at active zones of the larval NMJ in endophilin animals...
- The endocytic machinery in nerve terminals surrounds sites of exocytosisJ Roos
Department of Biochemistry and Biophysics, Hormone Research Institute, University of California, San Francisco, San Francisco, 94143 0534, USA
Curr Biol 9:1411-4. 1999....
- Essential role of endophilin A in synaptic vesicle budding at the Drosophila neuromuscular junctionAntoine Guichet
Laboratory of Developmental Biology, Institut Jacques Monod, 2 place Jussieu, F 75251 Paris Cedex 05, France
EMBO J 21:1661-72. 2002..of the neuromuscular junction (NMJ) of mutant L3 larvae carrying the temperature-sensitive allele of dynamin, shibire. D-endoA null mutants show severe defects in motility and die at the early L2 larval stage...
- Drosophila Amphiphysin is implicated in protein localization and membrane morphogenesis but not in synaptic vesicle endocytosisA C Zelhof
Institute of Neuroscience, HHMI, University of Oregon 1254, Eugene, OR 97403, USA
Development 128:5005-15. 2001..The strong misexpression phenotype coupled with the mild mutant and lack of phenotypes suggests that Amphiphysin acts redundantly with other proteins to organize specialized membrane domains within a diverse array of cell types...
- Multiple forms of dynamin are encoded by shibire, a Drosophila gene involved in endocytosisM S Chen
Cell Biology Group, Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545
Nature 351:583-6. 1991b>Dynamin was discovered in bovine brain tissue as a nucleotide-sensitive microtubule-binding protein of relative molecular mass 100,000...
- Dynamin-like protein encoded by the Drosophila shibire gene associated with vesicular trafficA M van der Bliek
Division of Biology, California Institute of Technology, Pasadena 91125
Nature 351:411-4. 1991..The gene encodes a protein that is highly similar to rat dynamin, 69% of the amino-acid sequence is identical...
- SNARE-complex disassembly by NSF follows synaptic-vesicle fusionJ T Littleton
Center for Learning and Memory and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 98:12233-8. 2001..In the absence of NSF activity, there are sufficient fusion-competent SNAREs to exocytose both the readily released and the reserve pool of synaptic vesicles...
- Reversible blockage of neurite development and growth cone formation in neuronal cultures of a temperature-sensitive mutant of DrosophilaY T Kim
Department of Biology, University of Iowa, Iowa City 52242
J Neurosci 7:3245-55. 1987....
- Reversible blockage of membrane retrieval and endocytosis in the garland cell of the temperature-sensitive mutant of Drosophila melanogaster, shibirets1T Kosaka
J Cell Biol 97:499-507. 1983..were investigated in a temperature-sensitive, single gene mutant of Drosophila melanogaster, shibirets1 (shi) and wild-type (Oregon-R)...
- Cellular identification of water gustatory receptor neurons and their central projection pattern in DrosophilaTsuyoshi Inoshita
Department of Biology, Graduate School of Sciences, Kyushu University, Ropponmatsu, Fukuoka 810 8560, Japan
Proc Natl Acad Sci U S A 103:1094-9. 2006..We show here that the water receptor neurons project to a specific region in the subesophageal ganglion, thus revealing the water taste sensory map in Drosophila...
- Functional diversity in the dynamin familyA M van der Bliek
Dept of Biological Chemistry, UCLA School of Medicine 90095 1737, USA
Trends Cell Biol 9:96-102. 1999The function of the GTPase dynamin has been discussed for several years. It clearly plays a role in vesicle budding, but, despite recent insights, precisely how it functions in this process is still a matter of debate...
- Neuroscience. The meaning of a miniP Verstreken
Department of Molecular and Human Genetics, Division of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA
Science 293:443-4. 2001
- Genetic evidence for an equilibrium between docked and undocked vesiclesJ Li
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, CA 94305 5345, USA
Philos Trans R Soc Lond B Biol Sci 354:299-306. 1999..Before presenting this data and the significance of the finding for the regulation of neurotransmission, we will briefly review the use of Drosophila genetics as a tool for dissecting synaptic transmission...
- MOLECULAR BIOLOGY OF DROSOPHILA LEARNING AND MEMORYRonald Davis; Fiscal Year: 2000....
- Drosophila as an Experimental Model for EpilepsyJ Troy Littleton; Fiscal Year: 2010..We propose to use Drosophila as a model system to explore the cellular and molecular mechanisms by which enhanced neuronal activity couples to modifications of synaptic connectivity during epilespy. ..
- INTEGRIN FUNCTION IN MEMORYRonald Davis; Fiscal Year: 2009..Insights for the improvement of the diagnosis and treatment of diseases of working memory may be gathered from these studies. ..
- Olfactory memory trace formation in DrosophilaRonald Davis; Fiscal Year: 2009..This will aid in our understanding of the diseases that affect memory formation. ..
- Systems Biology of Morphogenesis and Spatial Information FlowARTHUR LANDER; Fiscal Year: 2007..Such insights will have a broad impact on a variety of health-related areas including human development and birth defects, stem cells and regeneration, normal and tumor cell growth, and basic physiology. ..
- MOLECULAR BIOLOGY OF DROSOPHILA LEARNING AND MEMORYRonald Davis; Fiscal Year: 2005..This basic information is expected to be of general significance to learning in other species and of relevance to diseases that affect cognition. ..
- INTEGRIN FUNCTION IN MEMORYRonald Davis; Fiscal Year: 2003..In addition, the research may highlight possible points of molecular dysfunction underlying cognitive diseases. ..
- Regulated Gene Expression and Drosophila BehaviorRonald Davis; Fiscal Year: 2003..The techniques proposed here offer a general method for turning on gene activity both spatially and temporally in Drosophila. ..
- INTEGRIN FUNCTION IN MEMORYRonald Davis; Fiscal Year: 2007..Insights for the improvement of the diagnosis and treatment of diseases of working memory may be gathered from these studies. ..
- Olfactory memory trace formation in DrosophilaRonald Davis; Fiscal Year: 2007..These experiments will significantly increase our understanding of how memories form and the events that can potentially perturb normal memory formation. This will aid in our understanding of the diseases that affect memory formation. ..
- MOLECULAR BIOLOGY OF DROSOPHILA LEARNING AND MEMORYRonald Davis; Fiscal Year: 2009....
- Neuroadaptations to Ethanol in DrosophilaTherese Kosten; Fiscal Year: 2007..These studies will further our understanding of the genetic basis for ethanol sensitivity and tolerance, important parameters for susceptibility to alcoholism. ..
- Generation and validation of inducible Cre driver lines by enhancer trappingRonald Davis; Fiscal Year: 2007..This is the lesson learned from Drosophila genetics and there is every reason to expect it to hold in the mouse. ..
- Cyclic AMP Signaling and Bipolar DisorderRonald Davis; Fiscal Year: 2007..Public Health Relevance: Bipolar Disorder is a prevalent brain disorder. The studies proposed here will help to elucidate the genetic basis for the disease. ..
- NEUROBIOLOGICALLY IMPORTANT GENES IIRonald Davis; Fiscal Year: 1991....
- VISUAL SYSTEM DEVELOPMENT AND SYNAPTOGENESISIan Meinertzhagen; Fiscal Year: 2009..when transmission from photoreceptors is reversibly blocked by a temperature-sensitive construct of Drosophila dynamin. C...
- Endocytic Control of Notch-Mediated Cell Fate Decisions in NeurogenesisFABRICE J ROEGIERS; Fiscal Year: 2010....
- Motivational control of appetitive memoryScott Waddell; Fiscal Year: 2009....
- TRANSCRIPTIONAL DETERMINANTS OF B LYMPHOCYTESTHOMAS KADESCH; Fiscal Year: 2002....
- Transport and Complexity in Biological SystemsARTHUR LANDER; Fiscal Year: 2004..abstract_text> ..
- Circadian control of a behavioral outputJohn Ewer; Fiscal Year: 2007..Thus it offers a unique opportunity to elucidate how the activity of the clock is coupled to the mechanism that controls a behavioral output. ..
- Functional Anatomy of Learning and Memory in DrosophilaJOSHUA DUBNAU; Fiscal Year: 2007..to this proposal is the availability in flies of a Gal4-responsive temperature-sensitive (and dominant negative) dynamin transgene (UAS-Shi/ ts), which permits temperature dependent and reversible disruption of neuronal function...