Gene Symbol: scra
Description: scraps
Alias: ABP8, Abp8, Ani, Anil, Anillin, CG2092, Dmel\CG2092, Scra, ani, anil, anillin, l(2)03427, l(2)43Ec, l(2)k08255, scraps, Actin-binding protein 8, CG2092-PA, CG2092-PB, CG2092-PC, anillin, annilin, lethal (2) k08255, scra-PA, scra-PB, scra-PC
Species: fruit fly

Top Publications

  1. Goldbach P, Wong R, Beise N, Sarpal R, Trimble W, Brill J. Stabilization of the actomyosin ring enables spermatocyte cytokinesis in Drosophila. Mol Biol Cell. 2010;21:1482-93 pubmed publisher
    The scaffolding protein anillin is required for completion of cytokinesis...
  2. Giansanti M, Bucciarelli E, Bonaccorsi S, Gatti M. Drosophila SPD-2 is an essential centriole component required for PCM recruitment and astral-microtubule nucleation. Curr Biol. 2008;18:303-9 pubmed publisher
    ..These results indicate that DSpd-2 functions in a very early step of the PCM recruitment pathway. ..
  3. Giansanti M, Gatti M, Bonaccorsi S. The role of centrosomes and astral microtubules during asymmetric division of Drosophila neuroblasts. Development. 2001;128:1137-45 pubmed
    ..This suggests that astral microtubules mediate proper spindle rotation during neuroblast division. ..
  4. D Avino P, Takeda T, Capalbo L, Zhang W, Lilley K, Laue E, et al. Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site. J Cell Sci. 2008;121:1151-8 pubmed publisher
    b>Anillin, one of the first factors recruited to the cleavage site during cytokinesis, interacts with actin, myosin II and septins, and is essential for proper organization of the actomyosin contractile ring...
  5. de Cuevas M, Spradling A. Morphogenesis of the Drosophila fusome and its implications for oocyte specification. Development. 1998;125:2781-9 pubmed
    ..Based on these observations, we argue that the oocyte is specified at the first cyst division. ..
  6. D Avino P, Savoian M, Glover D. Cleavage furrow formation and ingression during animal cytokinesis: a microtubule legacy. J Cell Sci. 2005;118:1549-58 pubmed
    ..The same mechanism might be conserved in other organisms. ..
  7. Papoulas O, Hays T, Sisson J. The golgin Lava lamp mediates dynein-based Golgi movements during Drosophila cellularization. Nat Cell Biol. 2005;7:612-8 pubmed
    ..Our results provide new evidence that golgins promote dynein-based motility of Golgi membranes. ..
  8. Zavortink M, Contreras N, Addy T, Bejsovec A, Saint R. Tum/RacGAP50C provides a critical link between anaphase microtubules and the assembly of the contractile ring in Drosophila melanogaster. J Cell Sci. 2005;118:5381-92 pubmed
    ..and fail to localise the key cytokinetic components Pebble (a RhoGEF), Aurora B kinase, Diaphanous, Pav-KLP and Anillin. The GAP activity of Tum is required for cytokinesis: in its absence cytokinesis fails early even though Tum is ..
  9. Haglund K, Nezis I, Lemus D, Grabbe C, Wesche J, Liestøl K, et al. Cindr interacts with anillin to control cytokinesis in Drosophila melanogaster. Curr Biol. 2010;20:944-50 pubmed publisher
    ..In these structures, Cindr colocalizes with the essential cytokinesis regulator Anillin. We show that Cindr interacts with Anillin and that depletion of either Cindr or Anillin gives rise to binucleate ..

More Information


  1. Cabernard C, Prehoda K, Doe C. A spindle-independent cleavage furrow positioning pathway. Nature. 2010;467:91-4 pubmed publisher
    ..We show that early and late furrow proteins (Pavarotti, Anillin, and Myosin) are localized to the neuroblast basal cortex at anaphase onset by a Pins cortical polarity pathway, ..
  2. Lecuit T. Junctions and vesicular trafficking during Drosophila cellularization. J Cell Sci. 2004;117:3427-33 pubmed
    ..The novel proteins Nullo and Slam, which are specifically induced during cellularization, represent developmental regulators of membrane growth during cellularization. ..
  3. Bonaccorsi S, Giansanti M, Gatti M. Spindle self-organization and cytokinesis during male meiosis in asterless mutants of Drosophila melanogaster. J Cell Biol. 1998;142:751-61 pubmed
    ..In addition, these findings strongly suggest that the asters are not required for signaling cytokinesis. ..
  4. Royou A, Field C, Sisson J, Sullivan W, Karess R. Reassessing the role and dynamics of nonmuscle myosin II during furrow formation in early Drosophila embryos. Mol Biol Cell. 2004;15:838-50 pubmed
    ..We suggest that cellularization can be divided into two distinct processes: furrow ingression, driven by microtubule mediated vesicle delivery, and basal closure, which is mediated by actin/myosin based constriction. ..
  5. Heitzler P, Coulson D, Saenz Robles M, Ashburner M, Roote J, Simpson P, et al. Genetic and cytogenetic analysis of the 43A-E region containing the segment polarity gene costa and the cellular polarity genes prickle and spiny-legs in Drosophila melanogaster. Genetics. 1993;135:105-15 pubmed
    ..In addition 19 novel lethal complementation groups and two semi-lethal complementation groups with morphogenetic escaper phenotypes are described. ..
  6. Inoue Y, Savoian M, Suzuki T, Mathe E, Yamamoto M, Glover D. Mutations in orbit/mast reveal that the central spindle is comprised of two microtubule populations, those that initiate cleavage and those that propagate furrow ingression. J Cell Biol. 2004;166:49-60 pubmed
    ..orbit mutants have disorganized or incomplete anillin and actin rings, and although cleavage furrows initiate, they ultimately regress...
  7. Gregory S, Ebrahimi S, Milverton J, Jones W, Bejsovec A, Saint R. Cell division requires a direct link between microtubule-bound RacGAP and Anillin in the contractile ring. Curr Biol. 2008;18:25-9 pubmed
    ..a band of activated RhoA [6], which subsequently recruits contractile-ring components such as actin, myosin, and Anillin [1-3]...
  8. Somma M, Fasulo B, Cenci G, Cundari E, Gatti M. Molecular dissection of cytokinesis by RNA interference in Drosophila cultured cells. Mol Biol Cell. 2002;13:2448-60 pubmed
    ..We show that double-stranded RNAs for anillin, acGAP, pavarotti, rho1, pebble, spaghetti squash, syntaxin1A, and twinstar all disrupt cytokinesis in S2 tissue ..
  9. Lee D, Harris T. An Arf-GEF regulates antagonism between endocytosis and the cytoskeleton for Drosophila blastoderm development. Curr Biol. 2013;23:2110-20 pubmed publisher
    ..Endocytosis also regulates the PM and can be promoted or inhibited by cytoskeletal networks. However, endocytic regulation of the general membrane cytoskeleton is undocumented...
  10. Rothwell W, Zhang C, Zelano C, Hsieh T, Sullivan W. The Drosophila centrosomal protein Nuf is required for recruiting Dah, a membrane associated protein, to furrows in the early embryo. J Cell Sci. 1999;112 ( Pt 17):2885-93 pubmed
    ..Recruitment of actin, anillin and peanut to the furrows occurs normally in dah-derived embryos...
  11. Sisson J, Field C, Ventura R, Royou A, Sullivan W. Lava lamp, a novel peripheral golgi protein, is required for Drosophila melanogaster cellularization. J Cell Biol. 2000;151:905-18 pubmed
    ..were identified; seven have been previously implicated in cellularization and/or cytokinesis, including KLP3A, Anillin, Septins, and Dynamin. We now show that a novel MMAP, Lava Lamp (Lva), is also required for cellularization...
  12. Doberstein S, Fetter R, Mehta A, Goodman C. Genetic analysis of myoblast fusion: blown fuse is required for progression beyond the prefusion complex. J Cell Biol. 1997;136:1249-61 pubmed
    ..One of these genes, blown fuse, encodes a novel cytoplasmic protein expressed in unfused myoblasts that is essential for progression beyond the prefusion complex stage. ..
  13. Farkas R, Giansanti M, Gatti M, Fuller M. The Drosophila Cog5 homologue is required for cytokinesis, cell elongation, and assembly of specialized Golgi architecture during spermatogenesis. Mol Biol Cell. 2003;14:190-200 pubmed
    ..Our study suggests that Drosophila spermatogenesis can be an effective sensitized genetic system to uncover in vivo functions for proteins involved in Golgi architecture and/or vesicle transport. ..
  14. Prokopenko S, Brumby A, O KEEFE L, Prior L, He Y, Saint R, et al. A putative exchange factor for Rho1 GTPase is required for initiation of cytokinesis in Drosophila. Genes Dev. 1999;13:2301-14 pubmed
    ..Our results identify Pbl as a RhoGEF specifically required for cytokinesis and linked through Rho1 activity to the reorganization of the actin cytoskeleton at the cleavage furrow. ..
  15. Cao J, Albertson R, Riggs B, Field C, Sullivan W. Nuf, a Rab11 effector, maintains cytokinetic furrow integrity by promoting local actin polymerization. J Cell Biol. 2008;182:301-13 pubmed publisher
    ..We propose a model in which RE-derived vesicles promote furrow integrity by regulating the rate of actin polymerization through the RhoGEF2-Rho1 pathway. ..
  16. Hickson G, O Farrell P. Rho-dependent control of anillin behavior during cytokinesis. J Cell Biol. 2008;180:285-94 pubmed publisher
    b>Anillin is a conserved protein required for cytokinesis but its molecular function is unclear...
  17. Field C, Coughlin M, Doberstein S, Marty T, Sullivan W. Characterization of anillin mutants reveals essential roles in septin localization and plasma membrane integrity. Development. 2005;132:2849-60 pubmed
    b>Anillin is a conserved component of the contractile ring that is essential for cytokinesis, and physically interacts with three conserved cleavage furrow proteins, F-actin, myosin II and septins in biochemical assays...
  18. Robinson D, Smith Leiker T, Sokol N, Hudson A, Cooley L. Formation of the Drosophila ovarian ring canal inner rim depends on cheerio. Genetics. 1997;145:1063-72 pubmed
    ..Furthermore, this mutant reveals that the inner rim cytoskeleton is required for expansion of the ring canal opening and for plasma membrane stabilization. ..
  19. Ben El Kadhi K, Roubinet C, Solinet S, Emery G, Carreno S. The inositol 5-phosphatase dOCRL controls PI(4,5)P2 homeostasis and is necessary for cytokinesis. Curr Biol. 2011;21:1074-9 pubmed publisher
    ..Identification of dOCRL as essential for cell division may be important to understand the molecular basis of the phenotypic manifestations of Lowe syndrome. ..
  20. Eggert U, Kiger A, Richter C, Perlman Z, Perrimon N, Mitchison T, et al. Parallel chemical genetic and genome-wide RNAi screens identify cytokinesis inhibitors and targets. PLoS Biol. 2004;2:e379 pubmed
    ..Our study shows that parallel RNA interference and small molecule screening is a generally useful approach to identifying active small molecules and their target pathways...
  21. Sokac A, Wieschaus E. Zygotically controlled F-actin establishes cortical compartments to stabilize furrows during Drosophila cellularization. J Cell Sci. 2008;121:1815-24 pubmed publisher
    ..At the same time, cortical components that should concentrate in furrow canals, such as Myosin 2 (Zipper) and Anillin (Scraps), are missing from some furrows...
  22. Kitazawa D, Yamaguchi M, Mori H, Inoue Y. COPI-mediated membrane trafficking is required for cytokinesis in Drosophila male meiotic divisions. J Cell Sci. 2012;125:3649-60 pubmed
    ..Thus, we propose that COPI plays an important role in Drosophila male meiosis, not only through vesicle transport to the cleavage furrow region, but also through the formation of ER-based structures. ..
  23. Kechad A, Jananji S, Ruella Y, Hickson G. Anillin acts as a bifunctional linker coordinating midbody ring biogenesis during cytokinesis. Curr Biol. 2012;22:197-203 pubmed publisher
    ..Time-lapse microscopy of Drosophila S2 cells depleted of the scaffold protein Anillin [7-9] revealed that Anillin is required for complete closure of the CR and formation of the MR...
  24. Somers W, Saint R. A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis. Dev Cell. 2003;4:29-39 pubmed
  25. Kramerova I, Kramerov A. Mucinoprotein is a universal constituent of stable intercellular bridges in Drosophila melanogaster germ line and somatic cells. Dev Dyn. 1999;216:349-60 pubmed
  26. Rothwell W, Fogarty P, Field C, Sullivan W. Nuclear-fallout, a Drosophila protein that cycles from the cytoplasm to the centrosomes, regulates cortical microfilament organization. Development. 1998;125:1295-303 pubmed
    ..The nuf mutation does not disrupt anillin or peanut recruitment to the metaphase furrows indicating that Nuf is not involved in the signaling of metaphase ..
  27. O Farrell F, Kylsten P. Drosophila Anillin is unequally required during asymmetric cell divisions of the PNS. Biochem Biophys Res Commun. 2008;369:407-13 pubmed publisher
    ..We report a role of scraps, Drosophila Anillin, during these divisions...
  28. D Avino P, Savoian M, Capalbo L, Glover D. RacGAP50C is sufficient to signal cleavage furrow formation during cytokinesis. J Cell Sci. 2006;119:4402-8 pubmed
    ..Thus, the GAP component of centralspindlin is both necessary and sufficient for furrow formation and ingression and astral microtubules provide a route for its delivery to the cleavage site. ..
  29. Giansanti M, Belloni G, Gatti M. Rab11 is required for membrane trafficking and actomyosin ring constriction in meiotic cytokinesis of Drosophila males. Mol Biol Cell. 2007;18:5034-47 pubmed
    ..We propose that Gio and Fwd mediate Rab11 recruitment at the cleavage furrow and that Rab11 facilitates targeted membrane delivery to the advancing furrow. ..
  30. Adam J, Pringle J, Peifer M. Evidence for functional differentiation among Drosophila septins in cytokinesis and cellularization. Mol Biol Cell. 2000;11:3123-35 pubmed
    ..Together, the results suggest that some cell-division events in Drosophila do not require septin function, that there is functional differentiation among the Drosophila septins, or both. ..
  31. Liu J, Fairn G, Ceccarelli D, Sicheri F, Wilde A. Cleavage furrow organization requires PIP(2)-mediated recruitment of anillin. Curr Biol. 2012;22:64-9 pubmed publisher
    ..A key component of the membrane furrow is anillin. Upon targeting to the furrow through its pleckstrin homology (PH) domain, anillin acts as a scaffold linking the ..
  32. D Avino P, Savoian M, Glover D. Mutations in sticky lead to defective organization of the contractile ring during cytokinesis and are enhanced by Rho and suppressed by Rac. J Cell Biol. 2004;166:61-71 pubmed
    ..Together with an unusual distribution of F-actin and Anillin, these phenotypes are consistent with defective organization of the contractile ring...
  33. Straight A, Field C, Mitchison T. Anillin binds nonmuscle myosin II and regulates the contractile ring. Mol Biol Cell. 2005;16:193-201 pubmed
    We demonstrate that the contractile ring protein anillin interacts directly with nonmuscle myosin II and that this interaction is regulated by myosin light chain phosphorylation...
  34. Giansanti M, Bonaccorsi S, Gatti M. The role of anillin in meiotic cytokinesis of Drosophila males. J Cell Sci. 1999;112 ( Pt 14):2323-34 pubmed
    b>Anillin is a 190 kDa actin-binding protein that concentrates in the leading edges of furrow canals during Drosophila cellularization and in the cleavage furrow of both somatic and meiotic cells. We analyzed anillin behavior during D...
  35. Bucciarelli E, Giansanti M, Bonaccorsi S, Gatti M. Spindle assembly and cytokinesis in the absence of chromosomes during Drosophila male meiosis. J Cell Biol. 2003;160:993-9 pubmed
    ..This suggests that the association of Aurora B with chromosomes is not a prerequisite for its accumulation at the central spindle, or for its function during cytokinesis. ..
  36. Giansanti M, Farkas R, Bonaccorsi S, Lindsley D, Wakimoto B, Fuller M, et al. Genetic dissection of meiotic cytokinesis in Drosophila males. Mol Biol Cell. 2004;15:2509-22 pubmed
    ..Examination of preparations stained for tubulin, anillin, KLP3A, and F-actin revealed discrete defects in the components of the cytokinetic apparatus, suggesting that ..
  37. Thomas J, Wieschaus E. src64 and tec29 are required for microfilament contraction during Drosophila cellularization. Development. 2004;131:863-71 pubmed
    ..Double-mutant analysis between scraps, a mutation in anillin that eliminates microfilament rings, and bottleneck suggests that microfilaments can still contract even though ..
  38. Silverman Gavrila R, Hales K, Wilde A. Anillin-mediated targeting of peanut to pseudocleavage furrows is regulated by the GTPase Ran. Mol Biol Cell. 2008;19:3735-44 pubmed publisher
    ..We found that Ran was required for the localization of the septin Peanut to the pseudocleavage furrow, but not anillin or actin...
  39. Giansanti M, Bonaccorsi S, Williams B, Williams E, Santolamazza C, Goldberg M, et al. Cooperative interactions between the central spindle and the contractile ring during Drosophila cytokinesis. Genes Dev. 1998;12:396-410 pubmed
    ..These observations all indicate that proper actin assembly is necessary for centrosome separation and migration. ..
  40. Burgess R, Deitcher D, Schwarz T. The synaptic protein syntaxin1 is required for cellularization of Drosophila embryos. J Cell Biol. 1997;138:861-75 pubmed
    ..Thus, a single syntaxin can participate in trafficking steps that are functionally as distinct as synaptic transmission and cell division. ..
  41. Miller K, Kiehart D. Fly division. J Cell Biol. 1995;131:1-5 pubmed
  42. Field C, Alberts B. Anillin, a contractile ring protein that cycles from the nucleus to the cell cortex. J Cell Biol. 1995;131:165-78 pubmed
    ..M. Alberts. 1989. J. Cell Biol. 109:2963-2975). The cDNA encodes a 1201-amino acid protein which we name anillin. Anillin migrates at 190 kD on SDS-PAGE...
  43. Su J, Chow B, Boulianne G, Wilde A. The BAR domain of amphiphysin is required for cleavage furrow tip-tubule formation during cellularization in Drosophila embryos. Mol Biol Cell. 2013;24:1444-53 pubmed publisher
    ..These data suggest that regulating the recycling of membrane within the embryo is important in supporting timely furrow ingression. ..
  44. Ong S, Tan C. Germline cyst formation and incomplete cytokinesis during Drosophila melanogaster oogenesis. Dev Biol. 2010;337:84-98 pubmed publisher
    ..This work establishes a foundation for identifying genes specific for IC and for elucidating the molecular mechanism underlying this aspect of germline cyst formation. ..
  45. Naim V, Imarisio S, Di Cunto F, Gatti M, Bonaccorsi S. Drosophila citron kinase is required for the final steps of cytokinesis. Mol Biol Cell. 2004;15:5053-63 pubmed
    ..late telophases of both cell types displayed persistent midbodies associated with disorganized F actin and anillin structures...
  46. Brill J, Hime G, Scharer Schuksz M, Fuller M. A phospholipid kinase regulates actin organization and intercellular bridge formation during germline cytokinesis. Development. 2000;127:3855-64 pubmed
    ..Our results suggest a critical role for PI 4-kinases and phosphatidylinositol derivatives during the final stages of cytokinesis. ..
  47. Afshar K, Stuart B, Wasserman S. Functional analysis of the Drosophila diaphanous FH protein in early embryonic development. Development. 2000;127:1887-97 pubmed
    ..In addition, the dia mutant phenotype reveals a role for Diaphanous in recruitment of myosin II, anillin and Peanut to the cortical region between actin caps...
  48. Cooley L. Drosophila ring canal growth requires Src and Tec kinases. Cell. 1998;93:913-5 pubmed
  49. Mathe E, Inoue Y, Palframan W, Brown G, Glover D. Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions and development of the polarised microtubule network that interconnects oocyte and nurse cells during oogenesis. Development. 2003;130:901-15 pubmed
    ..The localisation of CLIP-190 to such microtubules and to the fusome is dependent upon Orbit/Mast to which it is complexed. ..
  50. Adams R, Tavares A, Salzberg A, Bellen H, Glover D. pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis. Genes Dev. 1998;12:1483-94 pubmed
    ..We show that the septin Peanut, actin, and the actin-associated protein Anillin, do not become correctly localized in pav mutants...
  51. Hime G, Brill J, Fuller M. Assembly of ring canals in the male germ line from structural components of the contractile ring. J Cell Sci. 1996;109 ( Pt 12):2779-88 pubmed least four proteins present at the contractile ring remain: the three septins (Pnut, Sep1 and Sep2) and anillin. In sharp contrast, in ovarian ring canals, septins have not been detected, anillin is lost from mature ring ..
  52. Ong S, Foote C, Tan C. Mutations of DMYPT cause over constriction of contractile rings and ring canals during Drosophila germline cyst formation. Dev Biol. 2010;346:161-9 pubmed publisher
    ..Our results suggest that there may be two parallel mechanisms to prevent the contractile rings from being completely closed, physical resistance and inhibition of myosin II activity via DMYPT. ..
  53. Kitazawa D, Matsuo T, Kaizuka K, Miyauchi C, Hayashi D, Inoue Y. Orbit/CLASP is required for myosin accumulation at the cleavage furrow in Drosophila male meiosis. PLoS ONE. 2014;9:e93669 pubmed publisher
    ..After MT contact, anillin and F-actin abruptly appeared on the equatorial cortex, simultaneously with myosin accumulation...
  54. Guerin C, Kramer S. RacGAP50C directs perinuclear gamma-tubulin localization to organize the uniform microtubule array required for Drosophila myotube extension. Development. 2009;136:1411-21 pubmed publisher