Gene Symbol: RpII140
Description: RNA polymerase II 140kD subunit
Alias: CG3180, DmRP140, Dmel\CG3180, Pol II, Pol IIc, PolII, PolIIc, RNA Pol II, RNA Pol II 140, RNA Pol IIc, RNA pol II, RNA polII, RNAP, RNAP II, RNApolII, RP140, RPB2_DROME, RPII140, RpII140[wimp], Rpb2, Rpll140, l(3)RplII140, pol II, polII, rpII140/wimp, wimp, RNA polymerase II 140kD subunit, 150kDa polypeptide, CG3180-PA, CG3180-PB, Pol II, RNA pol II second largest subunit, RNA polymerase II, RNA polymerase II 140 kD subunit, RNA polymerase II second largest subunit, RNA polymerase IIc, RNA polymerase subunit IIc, RNA polymeraseII-140 kd subunit, RpII140-PA, RpII140-PB, Rpb2, subunit IIc of RNA Pol II, wimp
Species: fruit fly

Top Publications

  1. Shore S, Byers S, Maury W, Price D. Identification of a novel isoform of Cdk9. Gene. 2003;307:175-82 pubmed
    Positive transcription factor b (P-TEFb) is required for RNA polymerase II to make the transition from abortive to productive elongation...
  2. Yao J, Munson K, Webb W, Lis J. Dynamics of heat shock factor association with native gene loci in living cells. Nature. 2006;442:1050-3 pubmed
    ..After heat shock, we have visualized the recruitment of RNA polymerase II (Pol II) to native hsp70 gene loci 87A and 87C in real time...
  3. Deuring R, Fanti L, Armstrong J, Sarte M, Papoulas O, Prestel M, et al. The ISWI chromatin-remodeling protein is required for gene expression and the maintenance of higher order chromatin structure in vivo. Mol Cell. 2000;5:355-65 pubmed
    ..The ISWI protein does not colocalize with RNA Pol II on salivary gland polytene chromosomes, suggesting a possible role for ISWI in transcriptional repression...
  4. Chen Y, Weeks J, Mortin M, Greenleaf A. Mapping mutations in genes encoding the two large subunits of Drosophila RNA polymerase II defines domains essential for basic transcription functions and for proper expression of developmental genes. Mol Cell Biol. 1993;13:4214-22 pubmed
    ..in genes encoding the largest (RpII215) and second-largest (RpII140) subunits of Drosophila melanogaster RNA polymerase II. Using polymerase chain reaction (PCR) amplification and single-strand conformation polymorphism (SSCP) ..
  5. Srinivasan S, Armstrong J, Deuring R, Dahlsveen I, McNeill H, Tamkun J. The Drosophila trithorax group protein Kismet facilitates an early step in transcriptional elongation by RNA Polymerase II. Development. 2005;132:1623-35 pubmed
    ..with virtually all sites of transcriptionally active chromatin in a pattern that largely overlaps that of RNA Polymerase II (Pol II)...
  6. Skantar A, Greenleaf A. Identifying a transcription factor interaction site on RNA polymerase II. Gene Expr. 1995;5:49-69 pubmed
    ..The same fusion proteins similarly inhibit dTFIIF stimulation of Pol II elongation on dC-tailed templates, suggesting that the IIc(A519-G992) fragment, which carries conserved regions D-..
  7. Liu R, Abreu Blanco M, Barry K, Linardopoulou E, Osborn G, Parkhurst S. Wash functions downstream of Rho and links linear and branched actin nucleation factors. Development. 2009;136:2849-60 pubmed publisher
    ..Our results establish Wash and Rho as regulators of both linear- and branched-actin networks, and suggest an Arp2/3-mediated mechanism for how cells might coordinately regulate these structures. ..
  8. Magie C, Parkhurst S. Rho1 regulates signaling events required for proper Drosophila embryonic development. Dev Biol. 2005;278:144-54 pubmed
  9. Srinivasan L, Atchison M. YY1 DNA binding and PcG recruitment requires CtBP. Genes Dev. 2004;18:2596-601 pubmed
    ..These results reveal a new role for CtBP in controlling YY1 DNA binding and recruitment of PcG proteins to DNA. ..

More Information


  1. Morcillo P, MacIntyre R. Genetic and molecular characterization of a variegating hsp70-acZ fusion gene in the euchromatic 31 B region of Drosophila melanogaster. Genome. 2001;44:698-707 pubmed
    ..However, other modifiers of PEV did not affect the expression pattern of the gene. These results show a novel euchromatic tissue-specific variegation that is not associated with classical heterochromatic PEV. ..
  2. Shaiu W, Hsieh T. Targeting to transcriptionally active loci by the hydrophilic N-terminal domain of Drosophila DNA topoisomerase I. Mol Cell Biol. 1998;18:4358-67 pubmed
    ..The top1-lacZ fusion proteins colocalized with RNA polymerase II (pol II) at developmental puffs on the polytene chromosomes...
  3. TenHarmsel A, Biggin M. Bending DNA can repress a eukaryotic basal promoter and inhibit TFIID binding. Mol Cell Biol. 1995;15:5492-8 pubmed
    ..binding and leads to the formation of a DNA loop which encompasses the DNA sequences normally bound by the RNA polymerase II general transcription factors...
  4. Wampler S, Tyree C, Kadonaga J. Fractionation of the general RNA polymerase II transcription factors from Drosophila embryos. J Biol Chem. 1990;265:21223-31 pubmed
    We have subdivided the components of the basic RNA polymerase II machinery from Drosophila embryos into three fractions and RNA polymerase II. The RNA polymerase II was 90% homogeneous and possessed the IIa form of the largest subunit...
  5. Chen Y, Chafin D, Price D, Greenleaf A. Drosophila RNA polymerase II mutants that affect transcription elongation. J Biol Chem. 1996;271:5993-9 pubmed
    We have examined the properties of two Drosophila RNA polymerase II mutants, C4 and S1, during elongation, pyrophosphorolysis, and DmS-II-stimulated transcript cleavage...
  6. Austin R, Biggin M. Purification of the Drosophila RNA polymerase II general transcription factors. Proc Natl Acad Sci U S A. 1996;93:5788-92 pubmed
    We describe a fractionation and purification scheme for the Drosophila RNA polymerase II general transcription factors...
  7. Abreu Blanco M, Verboon J, Parkhurst S. Coordination of Rho family GTPase activities to orchestrate cytoskeleton responses during cell wound repair. Curr Biol. 2014;24:144-55 pubmed publisher
    ..The cell wound repair response is an example of how specific pathways can be activated locally in response to the cell's needs. ..
  8. Krasnoselskaya I, Huang J, Jones T, Dezan C, Mortin M. Selection and analysis of rare second-site suppressors of Drosophila RNA polymerase II mutations. Mol Gen Genet. 1998;258:457-65 pubmed
    ..More than 11 million flies mutant for one of five recessive-lethal mutations in the two largest subunits of RNA polymerase II were selected for additional mutations that restored viability...
  9. Mounkes L, Fuller M. The DUG gene of Drosophila melanogaster encodes a structural and functional homolog of the S. cerevisiae SUG1 predicted ATPase associated with the 26S proteasome. Gene. 1998;206:165-74 pubmed
  10. Gilmour D, Dietz T, Elgin S. UV cross-linking identifies four polypeptides that require the TATA box to bind to the Drosophila hsp70 promoter. Mol Cell Biol. 1990;10:4233-8 pubmed
    ..Both the extended footprint and the polypeptides identified by UV cross-linking indicate that the Drosophila TATA factor is a multicomponent complex. ..
  11. Magie C, Meyer M, Gorsuch M, Parkhurst S. Mutations in the Rho1 small GTPase disrupt morphogenesis and segmentation during early Drosophila development. Development. 1999;126:5353-64 pubmed
    ..We also show that Rho1 interacts both genetically and physically with concertina, a G(alpha) protein involved in cell shape changes during gastrulation. ..
  12. Kontermann R, Sitzler S, Seifarth W, Petersen G, Bautz E. Primary structure and functional aspects of the gene coding for the second-largest subunit of RNA polymerase III of Drosophila. Mol Gen Genet. 1989;219:373-80 pubmed
    ..The protein sequence features the same regions of similarity as observed for the corresponding subunits of RNA polymerase II of Drosophila and yeast and the Escherichia coli beta subunit...
  13. Kal A, Mahmoudi T, Zak N, Verrijzer C. The Drosophila brahma complex is an essential coactivator for the trithorax group protein zeste. Genes Dev. 2000;14:1058-71 pubmed
    ..These results establish that different chromatin remodeling factors display distinct functional properties and provide novel insights into the mechanism of their targeting. ..
  14. Schock F, Sauer F, Jackle H, Purnell B. Drosophila head segmentation factor buttonhead interacts with the same TATA box-binding protein-associated factors and in vivo DNA targets as human Sp1 but executes a different biological program. Proc Natl Acad Sci U S A. 1999;96:5061-5 pubmed
  15. Poortinga G, Watanabe M, Parkhurst S. Drosophila CtBP: a Hairy-interacting protein required for embryonic segmentation and hairy-mediated transcriptional repression. EMBO J. 1998;17:2067-78 pubmed
    ..While Hairy is probably not the only segmentation gene interacting with dCtBP, we show dose-sensitive genetic interactions between dCtBP and hairy mutations. ..
  16. Kontermann R, Bautz E. Similarity between subunit 8 of yeast RNA polymerase II (RPB8) and the second-largest subunits of eukaryotic RNA polymerases. Nucleic Acids Res. 1992;20:5231 pubmed
  17. Hamilton B, Mortin M, Greenleaf A. Reverse genetics of Drosophila RNA polymerase II: identification and characterization of RpII140, the genomic locus for the second-largest subunit. Genetics. 1993;134:517-29 pubmed
    We have used a reverse genetics approach to isolate genes encoding two subunits of Drosophila melanogaster RNA polymerase II. RpII18 encodes the 18-kDa subunit and maps cytogenetically to polytene band region 83A...
  18. Visa N, Diez J, Santa Cruz M. Comparative analysis of four parameters involved in puffing activity along chromosome arm 2L of D melanogaster. Biol Cell. 1991;73:71-8 pubmed
    ..On the other hand, puffs of similar size incorporate 3H-uridine at quite different rates. The presence of RNA polymerase II seems to follow a coincident pattern with that of 3H-uridine incorporation...
  19. Park J, Werner J, Kim J, Lis J, Kim Y. Mediator, not holoenzyme, is directly recruited to the heat shock promoter by HSF upon heat shock. Mol Cell. 2001;8:9-19 pubmed
    Activators of RNA polymerase II (Pol II) transcription have been shown to bind several coactivators and basal factors in vitro...
  20. Zhang Z, Wu C, Gilmour D. Analysis of polymerase II elongation complexes by native gel electrophoresis. Evidence for a novel carboxyl-terminal domain-mediated termination mechanism. J Biol Chem. 2004;279:23223-8 pubmed
    ..approach using native gel electrophoresis for studying interactions of elongation factors with isolated Pol II elongation complexes. The gel distinguishes Pol IIA and Pol IIB containing complexes...
  21. Zhang Z, Gilmour D. Pcf11 is a termination factor in Drosophila that dismantles the elongation complex by bridging the CTD of RNA polymerase II to the nascent transcript. Mol Cell. 2006;21:65-74 pubmed
    The mechanism by which Pol II terminates transcription in metazoans is not understood. We show that Pcf11 is directly involved in termination in Drosophila...
  22. Spencer C, Groudine M. Transcription elongation and eukaryotic gene regulation. Oncogene. 1990;5:777-85 pubmed
    Each step in the synthesis of functional transcript by RNA polymerase II provides a level at which gene expression can be regulated...
  23. Zraly C, Marenda D, Nanchal R, Cavalli G, Muchardt C, Dingwall A. SNR1 is an essential subunit in a subset of Drosophila brm complexes, targeting specific functions during development. Dev Biol. 2003;253:291-308 pubmed
    ..Thus, SNR1 is essential for some, but not all Brm functions, and it likely serves as an optional subunit, directing Brm complex activity to specific gene loci or cellular processes. ..
  24. Wiedemann M, Oldenburg I, Sitzler S, Petersen G. Transcription of DmRP140, the gene coding for the second-largest subunit of RNA polymerase II. Biochim Biophys Acta. 1997;1350:282-6 pubmed
    ..housekeeping genes, we have characterized the promoter of the gene coding for the second-largest subunit of RNA polymerase II (DmRP140)...
  25. Pérez Lluch S, Blanco E, Carbonell A, Raha D, Snyder M, Serras F, et al. Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing. Nucleic Acids Res. 2011;39:4628-39 pubmed publisher
    ..We have characterized the occupancy of phosphorylated forms of RNA Polymerase II and histone marks associated with activation and repression of transcription...
  26. Henikoff S, Furuyama T, Ahmad K. Histone variants, nucleosome assembly and epigenetic inheritance. Trends Genet. 2004;20:320-6 pubmed
  27. Jackson S, Blochlinger K. cut interacts with Notch and protein kinase A to regulate egg chamber formation and to maintain germline cyst integrity during Drosophila oogenesis. Development. 1997;124:3663-72 pubmed
    ..cut encodes a nuclear protein containing DNA-binding motifs, and we suggest that it participates in intercellular communications by regulating the expression of molecules that directly participate in this process. ..
  28. Mortin M. Use of second-site suppressor mutations in Drosophila to identify components of the transcriptional machinery. Proc Natl Acad Sci U S A. 1990;87:4864-8 pubmed
    ..Flies conditionally lethal because they carry mutations in the largest subunit of RNA polymerase II were mutagenized; ten million progeny were then screened for compensatory mutations...
  29. Breen T, Harte P. Molecular characterization of the trithorax gene, a positive regulator of homeotic gene expression in Drosophila. Mech Dev. 1991;35:113-27 pubmed
    ..The primary transcription unit is differentially spliced to produce two large transcripts of 12 and 15 kb that have different developmental profiles. ..
  30. Reim I, Mattow J, Saumweber H. The RRM protein NonA from Drosophila forms a complex with the RRM proteins Hrb87F and S5 and the Zn finger protein PEP on hnRNA. Exp Cell Res. 1999;253:573-86 pubmed
    ..Like NonA, X4/PEP, S5, and P11/Hrb87F are present on active sites on polytene chromosomes. The precipitated NonA complex is enriched for certain protein encoding RNAs, notably, histone H3 and H4 RNA. ..
  31. Abruzzi K, Rodriguez J, Menet J, Desrochers J, Zadina A, Luo W, et al. Drosophila CLOCK target gene characterization: implications for circadian tissue-specific gene expression. Genes Dev. 2011;25:2374-86 pubmed publisher
    ..About 30% of target genes also show cycling RNA polymerase II (Pol II) binding...
  32. Chao S, Fujinaga K, Marion J, Taube R, Sausville E, Senderowicz A, et al. Flavopiridol inhibits P-TEFb and blocks HIV-1 replication. J Biol Chem. 2000;275:28345-8 pubmed
    ..We found that the flavonoid potently inhibited transcription by RNA polymerase II in vitro by blocking the transition into productive elongation, a step controlled by P-TEFb...
  33. Leatherman J, Levin L, Boero J, Jongens T. germ cell-less acts to repress transcription during the establishment of the Drosophila germ cell lineage. Curr Biol. 2002;12:1681-5 pubmed
  34. Martin M, Medina F. A Drosophila anti-RNA polymerase II antibody recognizes a plant nucleolar antigen, RNA polymerase I, which is mostly localized in fibrillar centres. J Cell Sci. 1991;100 ( Pt 1):99-107 pubmed
    ..onion root meristematic cells has been studied by means of an antibody originally raised against Drosophila RNA polymerase II. This antibody recognizes the homologous domains of the large subunit of the enzyme, which are highly ..
  35. Rosenberg M, Parkhurst S. Drosophila Sir2 is required for heterochromatic silencing and by euchromatic Hairy/E(Spl) bHLH repressors in segmentation and sex determination. Cell. 2002;109:447-58 pubmed
    ..These results indicate that Sir2 in higher organisms plays an essential role in both euchromatic repression and heterochromatic silencing. ..
  36. Bentley D. RNA processing. A tale of two tails. Nature. 1998;395:21-2 pubmed
  37. Abreu Blanco M, Verboon J, Parkhurst S. Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling. J Cell Biol. 2011;193:455-64 pubmed publisher
    ..Our results show that single-cell wound repair requires specific spatial and temporal cytoskeleton responses with distinct components and mechanisms required at different stages of the process. ..
  38. Osheim Y, Sikes M, Beyer A. EM visualization of Pol II genes in Drosophila: most genes terminate without prior 3' end cleavage of nascent transcripts. Chromosoma. 2002;111:1-12 pubmed
    Transcription termination by RNA polymerase II (Pol II) on most mRNA-encoding genes is dependent on transcription through a functional poly(A) signal...
  39. Seifarth W, Petersen G, Kontermann R, Riva M, Huet J, Bautz E. Identification of the genes coding for the second-largest subunits of RNA polymerases I and III of Drosophila melanogaster. Mol Gen Genet. 1991;228:424-32 pubmed
    ..with the second-largest subunit of RNA polymerase I but do not react with the respective subunits of RNA polymerase II and III. The second-largest subunit of RNA polymerase III is only recognized by anti-DmRP128...
  40. Rosales Nieves A, Johndrow J, Keller L, Magie C, Pinto Santini D, Parkhurst S. Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire and Cappuccino. Nat Cell Biol. 2006;8:367-76 pubmed
    ..We propose that Rho1, cappuccino and spire are elements of a conserved developmental cassette that is capable of directly mediating crosstalk between microtubules and microfilaments. ..
  41. Raisner R, Madhani H. Patterning chromatin: form and function for H2A.Z variant nucleosomes. Curr Opin Genet Dev. 2006;16:119-24 pubmed
    ..This chromatin pattern is generated through the action of a DNA deposition signal and a specific pattern of histone tail acetylation. ..
  42. Kim M, Mauro S, Gévry N, Lis J, Kraus W. NAD+-dependent modulation of chromatin structure and transcription by nucleosome binding properties of PARP-1. Cell. 2004;119:803-14 pubmed
    ..Thus, PARP-1 functions both as a structural component of chromatin and a modulator of chromatin structure through its intrinsic enzymatic activity. ..
  43. Blythe S, Wieschaus E. Zygotic genome activation triggers the DNA replication checkpoint at the midblastula transition. Cell. 2015;160:1169-81 pubmed publisher
    ..Measuring RNA polymerase II (Pol II) binding at 20 min intervals over the course of ZGA reveals that the checkpoint coincides with ..
  44. Sauer F, Hansen S, Tjian R. DNA template and activator-coactivator requirements for transcriptional synergism by Drosophila bicoid. Science. 1995;270:1825-8 pubmed
    ..Thus, contact between multiple activation domains of BCD and different targets within the TFIID complex can mediate transcriptional synergism. ..
  45. Breiling A, Turner B, Bianchi M, Orlando V. General transcription factors bind promoters repressed by Polycomb group proteins. Nature. 2001;412:651-5 pubmed
    ..We further show that PcG proteins interact in vitro with GTFs. We suggest that PcG complexes maintain silencing by inhibiting GTF-mediated activation of transcription. ..
  46. Mortin M, Zuerner R, Berger S, Hamilton B. Mutations in the second-largest subunit of Drosophila RNA polymerase II interact with Ubx. Genetics. 1992;131:895-903 pubmed
    Specific mutations in the gene encoding the largest subunit of RNA polymerase II (RpII215) cause a partial transformation of a structure of the third thoracic segment, the capitellum, into the analogous structure of the second thoracic ..
  47. Kavi H, Birchler J. Interaction of RNA polymerase II and the small RNA machinery affects heterochromatic silencing in Drosophila. Epigenetics Chromatin. 2009;2:15 pubmed publisher
    ..It has also been shown in fission yeast that the heterochromatin barrier is traversed by RNA Pol II and that the passage of RNA Pol II through heterochromatin is important for heterochromatin structure...
  48. Zeidler M, Yokomori K, Tjian R, Mlodzik M. Drosophila TFIIA-S is up-regulated and required during Ras-mediated photoreceptor determination. Genes Dev. 1996;10:50-9 pubmed
    ..These results are the first in vivo evidence for the coactivator function in transcriptional enhancement proposed for TFIIA. ..
  49. Falkenburg D, Dworniczak B, Faust D, Bautz E. RNA polymerase II of Drosophila. Relation of its 140,000 Mr subunit to the beta subunit of Escherichia coli RNA polymerase. J Mol Biol. 1987;195:929-37 pubmed
    We have determined the nucleotide sequence of the gene coding for the 140,000 Mr subunit of the DNA-dependent RNA polymerase II from Drosophila melanogaster. This analysis revealed features that are typical for a household-function gene...
  50. Visa N, Gonzalez Duarte R, Santa Cruz M. A cytological and molecular analysis of Adh gene expression in Drosophila melanogaster polytene chromosomes. Chromosoma. 1988;97:171-7 pubmed
    ..The presence of RNA polymerase II in this puff as well as its ability to incorporate tritiated uridine shows that it corresponds to a ..
  51. Ogasawara Y, Furuhashi H, Hirose S. DNA supercoiling factor positively regulates expression of the homeotic gene Abdominal-B in Drosophila melanogaster. Genes Cells. 2007;12:1347-55 pubmed
    ..Furthermore, preferential occupancy of SCF around transcription start sites of many active genes suggests a role for the factor in positive regulation of promoters. ..
  52. Seydoux G, Dunn M. Transcriptionally repressed germ cells lack a subpopulation of phosphorylated RNA polymerase II in early embryos of Caenorhabditis elegans and Drosophila melanogaster. Development. 1997;124:2191-201 pubmed
    ..mRNA production correlates with the absence of a specific phosphoepitope on the carboxy-terminal domain of RNA polymerase II. In both C...
  53. Sitzler S, Oldenburg I, Petersen G, Bautz E. Analysis of the promoter region of the housekeeping gene DmRP140 by sequence comparison of Drosophila melanogaster and Drosophila virilis. Gene. 1991;100:155-62 pubmed
    ..household genes, we have characterized the promoter of the gene coding for the second-largest subunit of RNA polymerase II (DmRP140)...
  54. Sandaltzopoulos R, Becker P. Heat shock factor increases the reinitiation rate from potentiated chromatin templates. Mol Cell Biol. 1998;18:361-7 pubmed
    Transcription by RNA polymerase II is highly regulated at the level of initiation and elongation...
  55. Weeks J, Hardin S, Shen J, Lee J, Greenleaf A. Locus-specific variation in phosphorylation state of RNA polymerase II in vivo: correlations with gene activity and transcript processing. Genes Dev. 1993;7:2329-44 pubmed
    ..CTDs), respectively, we have visualized the in vivo distributions of the differentially phosphorylated forms of Pol II on Drosophila polytene chromosomes...
  56. Rothe M, Wimmer E, Pankratz M, Gonzalez Gaitan M, Jackle H. Identical transacting factor requirement for knirps and knirps-related Gene expression in the anterior but not in the posterior region of the Drosophila embryo. Mech Dev. 1994;46:169-81 pubmed
  57. Peng J, Marshall N, Price D. Identification of a cyclin subunit required for the function of Drosophila P-TEFb. J Biol Chem. 1998;273:13855-60 pubmed
    ..elongation and is capable of phosphorylating the carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II. We cloned a cDNA encoding the large subunit of Drosophila P-TEFb and found the predicted protein contained ..
  58. Wang Z, Lindquist S. Developmentally regulated nuclear transport of transcription factors in Drosophila embryos enable the heat shock response. Development. 1998;125:4841-50 pubmed
    ..Restricted nuclear entry of HSF represents a newly described mechanism for regulating the heat-shock response. ..
  59. Kontermann R, Bautz E. Nucleic acid-binding regions of the second-largest subunit of Drosophila RNA polymerase II identified by southwestern blotting. FEBS Lett. 1994;344:166-70 pubmed
    ..Analysing overlapping bacterially expressed fragments of the second-largest subunit of Drosophila melanogaster RNA polymerase II in Southwestern DNA binding assays we have identified regions that have the potential to bind nucleic acids ..
  60. Tolhuis B, Blom M, Kerkhoven R, Pagie L, Teunissen H, Nieuwland M, et al. Interactions among Polycomb domains are guided by chromosome architecture. PLoS Genet. 2011;7:e1001343 pubmed publisher
    ..Our results demonstrate that many interactions among PcG target genes exist and that these interactions are guided by overall chromosome architecture. ..
  61. Rojas Ríos P, Guerrero I, González Reyes A. Cytoneme-mediated delivery of hedgehog regulates the expression of bone morphogenetic proteins to maintain germline stem cells in Drosophila. PLoS Biol. 2012;10:e1001298 pubmed publisher
  62. Greenleaf A. Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit. J Biol Chem. 1983;258:13403-6 pubmed
    A fragment of the Drosophila melanogaster RpIIC4 locus, which encodes the RNA polymerase II subunit that determines amanitin sensitivity, was inserted into a bacterial plasmid cloning vehicle useful for over-production of hybrid proteins...
  63. Kephart D, Marshall N, Price D. Stability of Drosophila RNA polymerase II elongation complexes in vitro. Mol Cell Biol. 1992;12:2067-77 pubmed
    We show that nuclear extract from Drosophila Kc cells supports efficient elongation by RNA polymerase II initiated from the actin 5C promoter. The addition of 0...
  64. Gutierrez L, Merino C, Vázquez M, Reynaud E, Zurita M. RNA polymerase II 140wimp mutant and mutations in the TFIIH subunit XPB differentially affect homeotic gene expression in Drosophila. Genesis. 2004;40:58-66 pubmed
    ..In this work, we report that mutations in hay and in the 140-kDa subunit of the RNA polymerase II (RpII140wimp) act as dominant modifiers of the derepression phenotypes of the Sex combs reduced (Scr) and ..
  65. Lis J. Promoter-associated pausing in promoter architecture and postinitiation transcriptional regulation. Cold Spring Harb Symp Quant Biol. 1998;63:347-56 pubmed
  66. Boehm A, Saunders A, Werner J, Lis J. Transcription factor and polymerase recruitment, modification, and movement on dhsp70 in vivo in the minutes following heat shock. Mol Cell Biol. 2003;23:7628-37 pubmed
    ..Here we examine the rapid changes upon heat shock in levels and location of heat shock factor (HSF), RNA polymerase II (Pol II) and its phosphorylated forms, and the Pol II kinase P-TEFb on hsp70 in vivo by using both real-time ..
  67. Peng J, Zhu Y, Milton J, Price D. Identification of multiple cyclin subunits of human P-TEFb. Genes Dev. 1998;12:755-62 pubmed
    ..factor b (P-TEFb) through phosphorylation of the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. Drosophila P-TEFb was identified recently as a cyclin-dependent kinase (CDK9) paired with a cyclin subunit (..
  68. Pham A, Muller S, Sauer F. Mesoderm-determining transcription in Drosophila is alleviated by mutations in TAF(II)60 and TAF(II)110. Mech Dev. 1999;84:3-16 pubmed
    ..The results provide evidence that TAF(II)-subunits within the TFIID complex play an important role during the molecular events leading to initiation of mesoderm formation in Drosophila. ..
  69. Leclerc V, Raisin S, Leopold P. Dominant-negative mutants reveal a role for the Cdk7 kinase at the mid-blastula transition in Drosophila embryos. EMBO J. 2000;19:1567-75 pubmed
    ..found in the transcription factor complex TFIIH, suggesting that it participates in vivo in the control of RNA polymerase II. We have examined the physiological role of Cdk7 during the course of Drosophila development...
  70. Kim W, Burke L, Mortin M. Molecular modeling of RNA polymerase II mutations onto DNA polymerase I. J Mol Biol. 1994;244:13-22 pubmed
    ..analysis in Drosophila melanogaster identifies eight suppressor mutations in the second largest subunit of RNA polymerase II. The suppressor mutations fall into two classes: five are strong, result from the same serine to cysteine ..
  71. Kontermann R, Kobor M, Bautz E. Identification of a nucleic acid-binding region within the largest subunit of Drosophila melanogaster RNA polymerase II. Protein Sci. 1993;2:223-30 pubmed
    ..expressed fusion proteins, we have identified a region of the largest, 215-kDa, subunit of Drosophila RNA polymerase II that has the potential to bind nucleic acids nonspecifically...
  72. Robertson S, Dockendorff T, Leatherman J, Faulkner D, Jongens T. germ cell-less is required only during the establishment of the germ cell lineage of Drosophila and has activities which are dependent and independent of its localization to the nuclear envelope. Dev Biol. 1999;215:288-97 pubmed
    ..These results indicate that gcl acts in at least two different ways during the establishment of the germ cell lineage. ..