robo

Summary

Gene Symbol: robo
Description: roundabout
Alias: BEST:GM07780, CG13521, CT32892, D-Robo1, Dmel\CG13521, ROBO, Robo, Robo-1, Robo1, dRobo, dRobo1, robo1, CG13521-PA, CG13521-PB, robo-PA, robo-PB, roundabout 1
Species: fruit fly

Top Publications

  1. ncbi Structural and functional analysis of slit and heparin binding to immunoglobulin-like domains 1 and 2 of Drosophila Robo
    Noemi Fukuhara
    Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom
    J Biol Chem 283:16226-34. 2008
  2. ncbi The Drosophila immunoglobulin gene turtle encodes guidance molecules involved in axon pathfinding
    Bader Al-Anzi
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Neural Dev 4:31. 2009
  3. ncbi Mutations affecting growth cone guidance in Drosophila: genes necessary for guidance toward or away from the midline
    M Seeger
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Neuron 10:409-26. 1993
  4. ncbi A novel member of the Ig superfamily, turtle, is a CNS-specific protein required for coordinated motor control
    K D Bodily
    Department of Biology, University of Utah, Salt Lake City, Utah 84112 0840
    J Neurosci 21:3113-25. 2001
  5. ncbi Vilse, a conserved Rac/Cdc42 GAP mediating Robo repulsion in tracheal cells and axons
    Annika Lundström
    Department of Developmental Biology, Wenner Gren Institute, Stockholm University, S 106 96 Stockholm, Sweden
    Genes Dev 18:2161-71. 2004
  6. ncbi Regulation of rho family GTPases is required to prevent axons from crossing the midline
    Janice L Fritz
    Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202, USA
    Dev Biol 252:46-58. 2002
  7. ncbi A Drosophila homolog of cyclase-associated proteins collaborates with the Abl tyrosine kinase to control midline axon pathfinding
    Zachary Wills
    Department of Cell Biology and Program in Neuroscience, Harvard Medical School, Boston, MA 02115, USA
    Neuron 36:611-22. 2002
  8. ncbi Projections of Drosophila multidendritic neurons in the central nervous system: links with peripheral dendrite morphology
    Wesley B Grueber
    Departments of Physiology and Biochemistry, University of California, San Francisco, Rock Hall, Room GD481, 1550 4th Street, San Francisco, CA 94143, USA
    Development 134:55-64. 2007
  9. ncbi Comm sorts robo to control axon guidance at the Drosophila midline
    Krystyna Keleman
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Cell 110:415-27. 2002
  10. ncbi Dephrin, a transmembrane ephrin with a unique structure, prevents interneuronal axons from exiting the Drosophila embryonic CNS
    Torsten Bossing
    Wellcome Trust Cancer Research UK Institute and Department of Genetics, Cambridge University, Tennis Court Road, Cambridge, CB2 1QR, UK
    Development 129:4205-18. 2002

Research Grants

  1. Regulation of Synapse Morphogenesis in Drosophila
    David L Van Vactor; Fiscal Year: 2010
  2. Molecular Genetics of Stem Cell in Drosophila
    Krishna Bhat; Fiscal Year: 2007
  3. Transcriptional Repression and Cofactor Recruitment
    Susan Parkhurst; Fiscal Year: 2007
  4. Genes Essential To Motor Axon Guidance in Drosophila
    David Van Vactor; Fiscal Year: 2007
  5. Mechanisms of Wound Healing in Drosophila
    Susan Parkhurst; Fiscal Year: 2007
  6. Mechanisms of Rho GTPase Function in Drosophila
    Susan Parkhurst; Fiscal Year: 2006
  7. Genetic Analysis of Heavy Metals Neurotoxicity
    Fen Biao Gao; Fiscal Year: 2005
  8. Mechanisms of Roundabout Repulsive Axon Guidance
    GREG BASHAW; Fiscal Year: 2007
  9. Regulation of Cell Migration by the APC-Microtubule Complex
    W Nelson; Fiscal Year: 2007
  10. Mechanisms of Slit-Roundabout Mediated Axon Repulsion
    Greg J Bashaw; Fiscal Year: 2010

Scientific Experts

Detail Information

Publications99

  1. ncbi Structural and functional analysis of slit and heparin binding to immunoglobulin-like domains 1 and 2 of Drosophila Robo
    Noemi Fukuhara
    Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom
    J Biol Chem 283:16226-34. 2008
    Recognition of the secreted protein Slit by transmembrane receptors of the Robo family provides important signals in the development of the nervous system and other organs, as well as in tumor metastasis and angiogenesis...
  2. ncbi The Drosophila immunoglobulin gene turtle encodes guidance molecules involved in axon pathfinding
    Bader Al-Anzi
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Neural Dev 4:31. 2009
    ..However, a large number of commissural axons successfully cross the midline even in the complete absence of netrin signaling, indicating the presence of a yet unidentified midline attractant...
  3. ncbi Mutations affecting growth cone guidance in Drosophila: genes necessary for guidance toward or away from the midline
    M Seeger
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Neuron 10:409-26. 1993
    ..Here we focus on two new genes: commissureless (comm) and roundabout (robo)...
  4. ncbi A novel member of the Ig superfamily, turtle, is a CNS-specific protein required for coordinated motor control
    K D Bodily
    Department of Biology, University of Utah, Salt Lake City, Utah 84112 0840
    J Neurosci 21:3113-25. 2001
    ..These phenotypes demonstrate that tutl plays an essential role in establishing a nervous system capable of executing coordinated motor output in complex behaviors...
  5. ncbi Vilse, a conserved Rac/Cdc42 GAP mediating Robo repulsion in tracheal cells and axons
    Annika Lundström
    Department of Developmental Biology, Wenner Gren Institute, Stockholm University, S 106 96 Stockholm, Sweden
    Genes Dev 18:2161-71. 2004
    Slit proteins steer the migration of many cell types through their binding to Robo receptors, but how Robo controls cell motility is not clear...
  6. ncbi Regulation of rho family GTPases is required to prevent axons from crossing the midline
    Janice L Fritz
    Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202, USA
    Dev Biol 252:46-58. 2002
    ..Midline repulsion appears to require down-regulation of Drac1 and Dcdc42 and activation of Rho...
  7. ncbi A Drosophila homolog of cyclase-associated proteins collaborates with the Abl tyrosine kinase to control midline axon pathfinding
    Zachary Wills
    Department of Cell Biology and Program in Neuroscience, Harvard Medical School, Boston, MA 02115, USA
    Neuron 36:611-22. 2002
    ..These studies identify Capulet as part of an emerging pathway linking guidance signals to regulation of cytoskeletal dynamics and suggest that the Abl pathway mediates signals downstream of multiple Roundabout receptors...
  8. ncbi Projections of Drosophila multidendritic neurons in the central nervous system: links with peripheral dendrite morphology
    Wesley B Grueber
    Departments of Physiology and Biochemistry, University of California, San Francisco, Rock Hall, Room GD481, 1550 4th Street, San Francisco, CA 94143, USA
    Development 134:55-64. 2007
    ..We have examined the effect of Robo family members on class-specific axon lamination, and have also taken a forward genetic approach to identify new ..
  9. ncbi Comm sorts robo to control axon guidance at the Drosophila midline
    Krystyna Keleman
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Cell 110:415-27. 2002
    Axon growth across the Drosophila midline requires Comm to downregulate Robo, the receptor for the midline repellent Slit...
  10. ncbi Dephrin, a transmembrane ephrin with a unique structure, prevents interneuronal axons from exiting the Drosophila embryonic CNS
    Torsten Bossing
    Wellcome Trust Cancer Research UK Institute and Department of Genetics, Cambridge University, Tennis Court Road, Cambridge, CB2 1QR, UK
    Development 129:4205-18. 2002
    ..We propose that the longitudinal tracts in the Drosophila CNS are moulded by a repulsive outer border of Dephrin expression...
  11. ncbi Commissureless is required both in commissural neurones and midline cells for axon guidance across the midline
    Marios Georgiou
    Molecular Neurobiology Group, MRC Centre for Developmental Neurobiology, Guy s Hospital Campus, King s College, London SE1 1UL, UK
    Development 129:2947-56. 2002
    ..Comm downregulates levels of Roundabout (Robo), a receptor for the midline repellent Slit, in order to allow axons to cross the midline...
  12. ncbi Independent functions of Slit-Robo repulsion and Netrin-Frazzled attraction regulate axon crossing at the midline in Drosophila
    David S Garbe
    Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Neurosci 27:3584-92. 2007
    ..receptors to attract commissural axons toward and across the midline, whereas Slit signals through Robo family receptors to prevent commissural axons from recrossing the midline, as well as to prevent ipsilateral axons ..
  13. ncbi Ectopic expression in the giant fiber system of Drosophila reveals distinct roles for roundabout (Robo), Robo2, and Robo3 in dendritic guidance and synaptic connectivity
    Tanja A Godenschwege
    Department of Biology, Morrill Science Center, University of Massachusetts, Amherst, Massachusetts 01003, USA
    J Neurosci 22:3117-29. 2002
    The Roundabout (Robo) receptors have been intensively studied for their role in regulating axon guidance in the embryonic nervous system, whereas a role in dendritic guidance has not been explored...
  14. ncbi Slit and Robo control the development of dendrites in Drosophila CNS
    Marie Pierre Furrer
    Department of Cell and Developmental Biology, University of Illinois, Urbana, IL 61801, USA
    Development 134:3795-804. 2007
    ..The diffusible signal molecule Slit and the neuronally expressed receptor Robo mediate growth cone collapse in vivo. However, in cultured neurons, these molecules promote dendritic development...
  15. ncbi Constitutively active myosin light chain kinase alters axon guidance decisions in Drosophila embryos
    You Seung Kim
    Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202, USA
    Dev Biol 249:367-81. 2002
    ..These results confirm that proper pathway formation requires careful regulation of MLCK and/or myosin II activity and suggest that regulation occurs in direct response to attractive and repulsive cues...
  16. ncbi A novel Dbl family RhoGEF promotes Rho-dependent axon attraction to the central nervous system midline in Drosophila and overcomes Robo repulsion
    G J Bashaw
    Department of Neuroscience, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Biol 155:1117-22. 2001
    ..far too many axons cross the midline, a phenotype reminiscent of loss of function mutations in the Roundabout (Robo) repulsive guidance receptor. Genetic analysis indicates that GEF64C expression can in fact overcome Robo repulsion...
  17. ncbi Son of sevenless directly links the Robo receptor to rac activation to control axon repulsion at the midline
    Long Yang
    Department of Neuroscience, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, Pennsylvania 19104, USA
    Neuron 52:595-607. 2006
    ..Intriguingly, the Ras and Rac-GEF activities of Sos can be uncoupled during Robo-mediated axon repulsion; Sos axon guidance function depends on its Rac-GEF activity, but not its Ras-GEF activity...
  18. ncbi Regulation of commissural axon pathfinding by slit and its Robo receptors
    Barry J Dickson
    Research Institute of Molecular Pathology IMP, A 1030 Vienna, Austria
    Annu Rev Cell Dev Biol 22:651-75. 2006
    ..as examples, we examine how commissural axon pathfinding is regulated by the Slit family of guidance cues and their Robo family receptors...
  19. ncbi Genetic specification of axonal arbors: atonal regulates robo3 to position terminal branches in the Drosophila nervous system
    Marta Zlatic
    Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, United Kingdom
    Neuron 37:41-51. 2003
    ....
  20. ncbi Comm function in commissural axon guidance: cell-autonomous sorting of Robo in vivo
    Krystyna Keleman
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3 5, A 1030 Vienna, Austria
    Nat Neurosci 8:156-63. 2005
    ..Comm) controls axon guidance across the Drosophila melanogaster midline by regulating surface levels of Robo, the receptor for the midline repellent Slit...
  21. ncbi Cytoplasmic domain requirements for Frazzled-mediated attractive axon turning at the Drosophila midline
    David S Garbe
    Department of Neuroscience, University of Pennsylvania School of Medicine, 1113 BRB2 3, 421 Curie Blvd, Philadelphia, PA 19104, USA
    Development 134:4325-34. 2007
    ....
  22. ncbi Axonal heparan sulfate proteoglycans regulate the distribution and efficiency of the repellent slit during midline axon guidance
    Karl G Johnson
    Harvard Medical School, Department of Cell Biology, Program in Neuroscience, 240 Longwood Avenue, Boston, MA 02115, USA
    Curr Biol 14:499-504. 2004
    ....
  23. ncbi ROBO directs axon crossing of segmental boundaries by suppressing responsiveness to relocalized Netrin
    Masaki Hiramoto
    Department of Developmental Genetics, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
    Nat Neurosci 9:58-66. 2006
    ..boundary, the longitudinal axons' responsiveness to Netrin presented on the commissure is suppressed by Roundabout (ROBO), through counteracting Gq signaling...
  24. ncbi Slit and Robo control cardiac cell polarity and morphogenesis
    Li Qian
    The Burnham Institute, Center for Neuroscienes and Aging, 10901 North Torrey Pines Road, La Jolla, California 92037, USA
    Curr Biol 15:2271-8. 2005
    ..The extracellular-matrix molecule Slit and its Robo-family receptors are conserved regulators of axonal guidance...
  25. ncbi The N-terminal and transmembrane domains of Commissureless are necessary for its function and trafficking within neurons
    Marios Georgiou
    Molecular Neurobiology Group, MRC Centre for Developmental Neurobiology, New Hunt s House, Guy s Campus, King s College, London SE1 1UL, UK
    Mech Dev 120:1009-19. 2003
    ..Comm is able to reduce cell surface levels of Roundabout (Robo), a receptor for the midline repellent Slit, on commissural axons and unknown inhibitors of synaptogenesis ..
  26. ncbi Slit stimulation recruits Dock and Pak to the roundabout receptor and increases Rac activity to regulate axon repulsion at the CNS midline
    Xueping Fan
    Department of Neuroscience, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104, USA
    Neuron 40:113-27. 2003
    Drosophila Roundabout (Robo) is the founding member of a conserved family of repulsive axon guidance receptors that respond to secreted Slit proteins...
  27. ncbi Abelson tyrosine kinase is required to transduce midline repulsive cues
    Anita Hsouna
    Department of Biological Sciences, Wayne State University, 5047 Gullen Mall, Detroit, Michigan 48202, USA
    J Neurobiol 57:15-30. 2003
    ..How Abl functions in this role is not yet clear, but we suggest that Abl may help regulate cytoskeletal dynamics underlying a growth cone's response to midline cues...
  28. ncbi Drosophila pod-1 crosslinks both actin and microtubules and controls the targeting of axons
    Michael E Rothenberg
    Department of Physiology, Howard Hughes Medical Institute, University of California, San Francisco, 533 Parnassus Avenue, San Francisco, CA 94143, USA
    Neuron 39:779-91. 2003
    ..Taken together, these results reveal novel activities for pod-1 and show that proper levels of Dpod1, an actin/MT crosslinker, must be maintained in the growth cone for correct axon guidance...
  29. ncbi Dendritic development: lessons from Drosophila and related branches
    Wesley B Grueber
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco, 533 Parnassus Avenue, Box 0725, San Francisco CA 94143, USA
    Curr Opin Neurobiol 14:74-82. 2004
    ..Here, we review some recent advances in our understanding of dendritic development in insects, focusing primarily on insights that have been gained from studies of Drosophila...
  30. ncbi Lateral positioning at the dorsal midline: Slit and Roundabout receptors guide Drosophila heart cell migration
    EDGARDO SANTIAGO-MARTINEZ
    Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Lane West, Piscataway, NJ 08854, USA
    Proc Natl Acad Sci U S A 103:12441-6. 2006
    ..study, we analyzed the role of Slit, an extracellular matrix protein and its transmembrane receptors Roundabout (Robo) and Roundabout2 (Robo2) during morphogenesis of the Drosophila heart tube, a process analogous to early heart ..
  31. ncbi Robo and Frazzled/DCC mediate dendritic guidance at the CNS midline
    Marie Pierre Furrer
    Department of Cell and Structural Biology, University of Illinois, 601 South Goodwin Avenue, Urbana, Illinois 61801, USA
    Nat Neurosci 6:223-30. 2003
    ..Using single-cell labeling, we describe specific guidance defects in dendrites of identified neurons in frazzled, robo, netrin and commissureless mutant embryos of Drosophila melanogaster...
  32. ncbi Short- and long-range repulsion by the Drosophila Unc5 netrin receptor
    K Keleman
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Neuron 32:605-17. 2001
    ..Ectopic expression of Unc5 on CNS axons can elicit either short- or long-range repulsion from the midline. Both short- and long-range repulsion require Netrin function, but only long-range repulsion requires Frazzled...
  33. ncbi Sequoia, a tramtrack-related zinc finger protein, functions as a pan-neural regulator for dendrite and axon morphogenesis in Drosophila
    J E Brenman
    Howard Hughes Medical Institute, Departments of Physiology and Biochemistry, University of California, San Francisco 94143, USA
    Dev Cell 1:667-77. 2001
    ....
  34. ncbi A frazzled/DCC-dependent transcriptional switch regulates midline axon guidance
    Long Yang
    Department of Neuroscience, University of Pennsylvania School of Medicine, 1113 BRB2 3, 421 Curie Boulevard, Philadelphia, PA 19104, USA
    Science 324:944-7. 2009
    ....
  35. ncbi Repulsive axon guidance: Abelson and Enabled play opposing roles downstream of the roundabout receptor
    G J Bashaw
    Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Cell 101:703-15. 2000
    Drosophila Roundabout (Robo) is the founding member of a conserved family of repulsive axon guidance receptors that respond to secreted Slit proteins...
  36. ncbi Structural homeostasis: compensatory adjustments of dendritic arbor geometry in response to variations of synaptic input
    Marco Tripodi
    Department of Zoology, University of Cambridge, Cambridge, United Kingdom
    PLoS Biol 6:e260. 2008
    ..The growing arbor thus counterbalances naturally occurring variations in synaptic density and activity so as to ensure that an appropriate level of input is achieved...
  37. ncbi Redundant mechanisms for regulation of midline crossing in Drosophila
    Giorgio F Gilestro
    Research Institute of Molecular Pathology, Vienna, Austria
    PLoS ONE 3:e3798. 2008
    ..In vertebrates and invertebrates regulation of crossing is achieved by interfering with Robo signalling either through sorting and degradation of the receptor, in flies, or through silencing of its repulsive ..
  38. ncbi Chimeric axon guidance receptors: the cytoplasmic domains of slit and netrin receptors specify attraction versus repulsion
    G J Bashaw
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Cell 97:917-26. 1999
    Frazzled (Fra) is the DCC-like Netrin receptor in Drosophila that mediates attraction; Roundabout (Robo) is a Slit receptor that mediates repulsion...
  39. ncbi Positional cues in the Drosophila nerve cord: semaphorins pattern the dorso-ventral axis
    Marta Zlatic
    Department of Zoology, University of Cambridge, Cambridge, United Kingdom
    PLoS Biol 7:e1000135. 2009
    ..The mediolateral positions of sensory arbors are controlled by the response of Robo receptors to a Slit gradient...
  40. ncbi Roundabout controls axon crossing of the CNS midline and defines a novel subfamily of evolutionarily conserved guidance receptors
    T Kidd
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Cell 92:205-15. 1998
    The robo gene in Drosophila was identified in a large-scale mutant screen for genes that control the decision by axons to cross the CNS midline. In robo mutants, too many axons cross and recross the midline...
  41. ncbi Slit proteins bind Robo receptors and have an evolutionarily conserved role in repulsive axon guidance
    K Brose
    Department of Anatomy, Howard Hughes Medical Institute, University of California, San Francisco 94143 0452, USA
    Cell 96:795-806. 1999
    ..to this paper, identifies the Slit protein as a candidate ligand for the repulsive guidance receptor Roundabout (Robo)...
  42. ncbi Slit is the midline repellent for the robo receptor in Drosophila
    T Kidd
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Cell 96:785-94. 1999
    Previous studies suggested that Roundabout (Robo) is a repulsive guidance receptor on growth cones that binds to an unknown midline ligand...
  43. ncbi Commissure formation in the embryonic CNS of Drosophila
    T Hummel
    Institut fur Neurobiologie, Universitat Munster, Badestrasse 9, D 48149 Munster, Germany
    Development 126:771-9. 1999
    ..The results lead to a detailed model relating different cellular functions to axonal patterning at the midline...
  44. ncbi Plexin A is a neuronal semaphorin receptor that controls axon guidance
    M L Winberg
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Cell 95:903-16. 1998
    ..Plexins, which themselves contain complete Semaphorin domains, may be both the ancestors of classical Semaphorins and binding partners for Semaphorins...
  45. ncbi Slit and Robo regulate dendrite branching and elongation of space-filling neurons in Drosophila
    Svetla Dimitrova
    Dendrite Differentiation, Department of Molecular Neurobiology, Max Planck Institute of Neurobiology, Munich, Germany
    Dev Biol 324:18-30. 2008
    ..In this study we show that a member of the conserved Robo receptor family, Robo, and its ligand Slit regulate the dendritic differentiation of space-filling neurons...
  46. ncbi Dosage-sensitive and complementary functions of roundabout and commissureless control axon crossing of the CNS midline
    T Kidd
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Neuron 20:25-33. 1998
    ..The robo;comm double-mutant phenotype is identical to robo alone, suggesting that in the absence of robo, comm is no longer ..
  47. ncbi The function of leak and kuzbanian during growth cone and cell migration
    K Schimmelpfeng
    Institut fur Neurobiologie, Universitat Munster, Badestrasse 9, 48149, Munster, Germany
    Mech Dev 106:25-36. 2001
    ..Our analyses support a model in which Kuzbanian mediates the proteolytic activation of the Slit/Roundabout receptor complex...
  48. ncbi Crossing the midline: roles and regulation of Robo receptors
    S Rajagopalan
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030, Vienna, Austria
    Neuron 28:767-77. 2000
    In the Drosophila CNS, the midline repellent Slit acts at short range through its receptor Robo to control midline crossing...
  49. ncbi Short-range and long-range guidance by Slit and its Robo receptors: a combinatorial code of Robo receptors controls lateral position
    J H Simpson
    Howard Hughes Medical Institute, Division of Neurobiology, Department of Molecular and Cell Biology, 519 LSA, University of California, Berkeley CA 94720, USA
    Cell 103:1019-32. 2000
    ..Here we show that a combinatorial code of Robo receptors controls lateral position in the CNS by responding to this presumptive Slit gradient...
  50. ncbi Selecting a longitudinal pathway: Robo receptors specify the lateral position of axons in the Drosophila CNS
    S Rajagopalan
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Cell 103:1033-45. 2000
    ..Local cues further refine the lateral position. Together, these long- and short-range guidance cues allow growth cones to select with precision a specific longitudinal pathway...
  51. ncbi Switching repulsion to attraction: changing responses to slit during transition in mesoderm migration
    S G Kramer
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, 519 Life Sciences Addition, University of California, Berkeley, CA 94720, USA
    Science 292:737-40. 2001
    Slit is secreted by cells at the midline of the central nervous system, where it binds to Roundabout (Robo) receptors and functions as a potent repellent...
  52. ncbi Short-range and long-range guidance by slit and its Robo receptors. Robo and Robo2 play distinct roles in midline guidance
    J H Simpson
    Howard Hughes Medical Institute, Division of Neurobiology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Neuron 28:753-66. 2000
    Previous studies showed that Roundabout (Robo) in Drosophila is a repulsive axon guidance receptor that binds to Slit, a repellent secreted by midline glia...
  53. ncbi Heparan sulfate proteoglycan syndecan promotes axonal and myotube guidance by slit/robo signaling
    Patrick Steigemann
    Abteilung Molekulare Entwicklungsbiologie, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, 37077 Goettingen, Germany
    Curr Biol 14:225-30. 2004
    Slit, the ligand for the Roundabout (Robo) receptors, is secreted from midline cells of the Drosophila central nervous system (CNS)...
  54. ncbi Genes regulating dendritic outgrowth, branching, and routing in Drosophila
    F B Gao
    Howard Hughes Medical Institute, Departments of Physiology and Biochemistry, University of California at San Francisco, San Francisco, California 94143 0725, USA
    Genes Dev 13:2549-61. 1999
    ..These findings begin to reveal the molecular mechanisms controlling dendritic morphogenesis...
  55. ncbi Drosophila Nedd4, a ubiquitin ligase, is recruited by Commissureless to control cell surface levels of the roundabout receptor
    Anna Myat
    Molecular Neurobiology Group, MRC Centre for Developmental Neurobiology, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, United Kingdom
    Neuron 35:447-59. 2002
    ..The ability of Commissureless to regulate Robo in the embryo also requires a Commissureless/DNedd4 interaction...
  56. ncbi Calmodulin and son of sevenless dependent signaling pathways regulate midline crossing of axons in the Drosophila CNS
    J L Fritz
    Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA
    Development 127:1991-2000. 2000
    ..These data suggest that Calmodulin and Son of sevenless signaling pathways function to interpret midline repulsive cues which prevent axons crossing the midline...
  57. ncbi Neurogenetic networks for startle-induced locomotion in Drosophila melanogaster
    Akihiko Yamamoto
    Department of Zoology, North Carolina State University, Raleigh, NC 27695, USA
    Proc Natl Acad Sci U S A 105:12393-8. 2008
    ..We find that behavioral and neuroanatomical phenotypes are determined by a common set of genes that are organized as partially overlapping genetic networks...
  58. ncbi Slit-roundabout signaling neutralizes netrin-Frazzled-mediated attractant cue to specify the lateral positioning of longitudinal axon pathways
    Krishna Moorthi Bhat
    Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322
    Genetics 170:149-59. 2005
    ..However, the midline Slit neutralizes this pathway in a Robo-dependent manner and prevents Netrin-Frazzled-mediated attraction of longitudinal tracts...
  59. ncbi Axon guidance: Comm hither, Robo
    Jessica Couch
    Department of Biology, 071 Gilmer Hall, University of Virginia, Charlottesville, VA 22903, USA
    Curr Biol 12:R741-2. 2002
    ..Recent studies have shed new light on the mechanism by which commissural axons change their sensitivity to guidance cues after crossing the midline...
  60. ncbi Axon guidance: the cytoplasmic tail
    Bharatkumar N Patel
    Program in Neuroscience, Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Curr Opin Cell Biol 14:221-9. 2002
    ..Many of these axon guidance receptors, including Plexin B, EphA, ephrin B and Robo, regulate the Rho family of GTPases, to effect changes in motility...
  61. ncbi Embryo morphogenesis: getting down to cells and molecules
    Lila Solnica-Krezel
    Vanderbilt University, VU Station B 351634, Nashville, TN 37235-1634, USA
    Development 130:4229-33. 2003
  62. ncbi Axon targeting meets protein trafficking: Comm takes Robo to the cleaners
    Mark Rosenzweig
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Dev Cell 3:301-2. 2002
    Axon guidance at the Drosophila midline relies on dynamic regulation of the guidance receptor Robo by its negative regulator Comm...
  63. ncbi Midline crossing by gustatory receptor neuron axons is regulated by fruitless, doublesex and the Roundabout receptors
    David J Mellert
    Department of Biology, Stanford University, Stanford, CA 94305, USA
    Development 137:323-32. 2010
    ..Finally, we (1) demonstrate that the roundabout (robo) paralogs also regulate midline crossing by these neurons, and (2) provide evidence that Fru(M) exerts its effect ..
  64. ncbi Drosophila central brain formation requires Robo proteins
    Emmanuelle Nicolas
    Institut Jacques Monod, Tour 42 43, 5éme étage 2 place jussieu, 75005 Paris, France
    Dev Genes Evol 215:530-6. 2005
    The Robo proteins have been extensively studied in the Drosophila embryonic ventral nerve cord, in which their expression level controls the midline crossing and optic lobe formation, but nothing is known about their activities during ..
  65. ncbi The egghead gene is required for compartmentalization in Drosophila optic lobe development
    Yun Fan
    Biozentrum Pharmazentrum, University of Basel, Klingelbergstrasse 50, CH 4056 Basel, Switzerland
    Dev Biol 287:61-73. 2005
    ..This finding underscores the importance of the lamina/lobula compartment boundary in R1-R6 axon targeting...
  66. ncbi Glial and neuronal functions of the Drosophila homolog of the human SWI/SNF gene ATR-X (DATR-X) and the jing zinc-finger gene specify the lateral positioning of longitudinal glia and axons
    Xuetao Sun
    Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada
    Genetics 173:1397-415. 2006
    ....
  67. ncbi Dendritic targeting in the leg neuropil of Drosophila: the role of midline signalling molecules in generating a myotopic map
    David J Brierley
    Medical Research Council MRC Centre for Developmental Neurobiology, King s College London, London, United Kingdom
    PLoS Biol 7:e1000199. 2009
    ..positioning of motoneuron dendrites in the leg neuropil is controlled by the midline signalling systems Slit-Robo and Netrin-Fra...
  68. ncbi The axon-guidance roundabout gene alters the pace of the Drosophila circadian clock
    Jimena Berni
    Laboratorio de Genética del Comportamiento, Fundacion Instituto Leloir, Instituto de Investigaciones Bioquímicas Buenos Aires IIBBA, CONICET, Av Patricias Argentinas 435, Buenos Aires 1405, Argentina
    Eur J Neurosci 27:396-407. 2008
    ..We have identified a viable allele of roundabout, robo(hy), where the period of locomotor activity is shortened...
  69. ncbi Repulsion by Slit and Roundabout prevents Shotgun/E-cadherin-mediated cell adhesion during Drosophila heart tube lumen formation
    EDGARDO SANTIAGO-MARTINEZ
    Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA
    J Cell Biol 182:241-8. 2008
    ..We show that Slit and its receptor Roundabout (Robo) are required at CB apical domains for lumen formation...
  70. ncbi NETRIN and SLIT guide salivary gland migration
    Tereza Kolesnikov
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Dev Biol 284:102-11. 2005
    ..We propose that analogous to axon guidance, a balance between chemo-attractants and chemo-repellents is required for the proper migratory path of the developing salivary glands...
  71. ncbi Binding site for Robo receptors revealed by dissection of the leucine-rich repeat region of Slit
    Jason A Howitt
    Department of Biological Sciences, Imperial College London, London, UK
    EMBO J 23:4406-12. 2004
    Recognition of the large secreted protein Slit by receptors of the Robo family provides fundamental signals in axon guidance and other developmental processes...
  72. ncbi Neurons and glia: team players in axon guidance
    Carole Chotard
    National Institute for Medical Research, Division of Molecular Neurobiology, The Ridgeway, London NW7 1AA, UK
    Trends Neurosci 27:655-61. 2004
    ..Equally, neurons provide crucial information to glia, supporting their migration and correct positioning...
  73. ncbi Lola regulates midline crossing of CNS axons in Drosophila
    Daniel Crowner
    Division of Basic Sciences, Program in Developmental Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave, N, Seattle, WA 98109, USA
    Development 129:1317-25. 2002
    ..it acts, in part, by augmenting the expression both of the midline repellant, Slit, and of its axonal receptor, Robo. Lola is thus the examplar of a class of axon guidance molecules that control axon patterning by coordinating the ..
  74. ncbi Slit signaling promotes the terminal asymmetric division of neural precursor cells in the Drosophila CNS
    B Mehta
    Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Development 128:3161-8. 2001
    ..Here, we show that the midline repellent Slit (Sli), via its receptor Roundabout (Robo), promotes the terminal asymmetric division of GMCs...
  75. ncbi Repellent signaling by Slit requires the leucine-rich repeats
    R Battye
    Department of Biology, McMaster University, Hamilton Ontario, L8S 4K1 Canada
    J Neurosci 21:4290-8. 2001
    ..Here we demonstrate that the LRR is required for repellent signaling and in vitro binding to Robo. Repellent signaling by slit is reduced by point mutations that encode single amino acid changes in the LRR domain...
  76. ncbi Neurobiology. The come-hither, don't-touch-me proteins
    G Vogel
    Science 290:2243-4. 2000
    Two newly discovered receptors, Slit and Roundabout (Robo), help determine how far axons in developing fruit flies travel after they cross the line between the fly's left and right sides...
  77. ncbi To cross or not to cross: a genetic analysis of guidance at the midline
    G Tear
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Perspect Dev Neurobiol 1:183-94. 1993
    ..These phenotypes suggest that these two genes encode important components for growth cone guidance at the midline...
  78. ncbi Developmental biology. Post-expressionist flies
    S Bray
    Nature 407:314-5. 2000
  79. ncbi Putting on the brakes: a negative regulatory function for Ena/VASP proteins in cell migration
    L M Machesky
    School of Biosciences, Division of Molecular and Cell Biology, University of Birmingham, United Kingdom
    Cell 101:685-8. 2000
  80. ncbi Axon pathfinding proceeds normally despite disrupted growth cone decisions at CNS midline
    B D Wolf
    Department of Cell and Structural Biology and Neuroscience Program, University of Illinois, Urbana, IL 61801, USA
    Development 127:2001-9. 2000
    ..Thus, neither the growth cones' particular molecular experience nor the decision at the midline caused by these mutations affects their ability to respond normally to subsequently presented cues...
  81. ncbi Slit proteins: key regulators of axon guidance, axonal branching, and cell migration
    K Brose
    Departments of Biochemistry and Biophysics, University of California, San Francisco, CA 94143 0452, USA
    Curr Opin Neurobiol 10:95-102. 2000
    ....
  82. ncbi Signal transduction underlying growth cone guidance by diffusible factors
    H J Song
    Molecular Neurobiology Laboratory, Howard Hughes Medical Institute at the Salk Institute, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Curr Opin Neurobiol 9:355-63. 1999
    ..Furthermore, many diffusible guidance cues appear to share common cytoplasmic signaling pathways...
  83. ncbi The middle and the end: slit brings guidance and branching together in axon pathway selection
    D Van Vactor
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Neuron 22:649-52. 1999
  84. ncbi Axon repulsion from the midline of the Drosophila CNS requires slit function
    R Battye
    Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada
    Development 126:2475-81. 1999
    ..slit interacts genetically with roundabout, which encodes a putative receptor for growth cone repulsion...
  85. ncbi Reduced adaptation of a non-recombining neo-Y chromosome
    Doris Bachtrog
    Institute of Cell, Animal and Population Biology, University of Edinburgh, UK
    Nature 416:323-6. 2002
    ..Our results provide evidence for the importance of sexual recombination for increasing and maintaining the level of adaptation of a population...
  86. ncbi Integrins regulate responsiveness to slit repellent signals
    Adrienne Stevens
    Department of Biology, McMaster University, Hamilton, Ontario, L8S 4K1, Canada
    J Neurosci 22:4448-55. 2002
    ..We propose that the strength of adhesive signaling from integrins influences the threshold of response by growth cones to repellent axon guidance cues...
  87. ncbi Roundabout 2 regulates migration of sensory neurons by signaling in trans
    Rachel Kraut
    Division of Biology 114 96, California Institute of Technology, Pasadena, CA 91125, USA
    Curr Biol 14:1319-29. 2004
    Roundabout (Robo) receptors and their ligand Slit are important regulators of axon guidance and cell migration...
  88. ncbi Rhomboid 3 orchestrates Slit-independent repulsion of tracheal branches at the CNS midline
    Marco Gallio
    Department of Developmental Biology, Wenner Gren Institute, Stockholm University, S 106 96 Stockholm, Sweden
    Development 131:3605-14. 2004
    ..We propose that Egfr activation provides a necessary switch for the interpretation of a yet unknown repellent function of the midline...
  89. ncbi The microtubule plus end tracking protein Orbit/MAST/CLASP acts downstream of the tyrosine kinase Abl in mediating axon guidance
    Haeryun Lee
    Department of Cell Biology, Program in Neuroscience, Dana Farber Cancer Institute Harvard Cancer Center and Harvard Center of Neurodegeneration and Repair, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Neuron 42:913-26. 2004
    ..These and other data suggest a model where Abl acts as a central signaling node to coordinate actin and microtubule dynamics downstream of guidance receptors...
  90. ncbi Slit-Robo signalling prevents sensory cells from crossing the midline in Drosophila
    Virginie Orgogozo
    Ecole Normale Superieure, UMR 8542, 46 rue d Ulm, 75005 Paris, France
    Mech Dev 121:427-36. 2004
    ..Our observations indicate that in slit mutant embryos, as well as in robo, robo2 double mutants, sensory precursor cells of the left and right vmd1a neurons aberrantly cluster at the ..
  91. ncbi The immunoglobulin superfamily in Caenorhabditis elegans and Drosophila melanogaster
    Oliver Hobert
    Development 131:2237-8; author reply 2238-40. 2004
  92. ncbi Movement through Slits: cellular migration via the Slit family
    Michael Piper
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia
    Bioessays 25:32-8. 2003
    ..This review focuses on the non-neuronal activities of Slit proteins, highlighting a common role for the Slit family in cellular migration...
  93. ncbi Tube morphogenesis: closure, but many openings remain
    W James Nelson
    Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA 94305 5435, USA
    Trends Cell Biol 13:615-21. 2003
    ..This review provides a summary of these pathways based upon articles published in the Tube Morphogenesis Series in Trends in Cell Biology...
  94. ncbi Metalloproteases: carving out a role in axon guidance
    Sarah McFarlane
    University of Calgary, Genes and Development Research Group, HSC 2207, 3330 Hospital Drive, NW, Calgary, Alberta, Canada T2N 4N1
    Neuron 37:559-62. 2003
    ..Culture studies suggest that this requirement for metalloprotease activity results from their ability to cleave ligands, or their receptors, so as to activate or inhibit specific axon extension or guidance signaling pathways...
  95. ncbi Fly developments: an introspective
    Susan Parkhurst
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle WA 98109, USA
    Bioessays 25:87-9. 2003
  96. ncbi Attraction versus repulsion: modular receptors make the difference in axon guidance
    M A Seeger
    Department of Molecular Genetics, Ohio State University, Columbus 43210, USA
    Cell 97:821-4. 1999
  97. ncbi Attractive and repulsive functions of Slit are mediated by different receptors in the Drosophila trachea
    Camilla Englund
    Umea Centre for Molecular Pathogenesis, Umea University, Sweden
    Development 129:4941-51. 2002
    ..b>Robo and Robo2 are expressed in different branches, and are both necessary for the correct orientation of branch ..
  98. ncbi Genetic and molecular dissection of axon pathfinding in the Drosophila nervous system
    M A Seeger
    Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104 5073
    Curr Opin Neurobiol 4:56-62. 1994
    ....
  99. ncbi Roundabout gene family functions during sensory axon guidance in the drosophila embryo are mediated by both Slit-dependent and Slit-independent mechanisms
    Linda Parsons
    Department of Anatomy and Cell Biology, University of Melbourne, Victoria 3010, Australia
    Dev Biol 264:363-75. 2003
    roundabout (robo) family genes play key roles in axon guidance in a wide variety of animals. We have investigated the roles of the robo family members, robo, robo2, and robo3, in the guidance of sensory axons in the Drosophila embryo...

Research Grants72

  1. Regulation of Synapse Morphogenesis in Drosophila
    David L Van Vactor; Fiscal Year: 2010
    ..We will determine the strategy by which Drosophila miR-8 promotes synapse formation as a model to better define the logic of pathways upstream and downstream of synaptic microRNAs. ..
  2. Molecular Genetics of Stem Cell in Drosophila
    Krishna Bhat; Fiscal Year: 2007
    ..These studies will help understand pathways that govern the self-renewing and terminal asymmetric division of precursor cells in multi-cellular organisms. ..
  3. Transcriptional Repression and Cofactor Recruitment
    Susan Parkhurst; Fiscal Year: 2007
    ..Results obtained from these projects are expected to have wide implications as HES family proteins and mechanisms of transcriptional repression are conserved throughout organisms. ..
  4. Genes Essential To Motor Axon Guidance in Drosophila
    David Van Vactor; Fiscal Year: 2007
    ..We will use a combination of genetics, cell biology and biochemistry to determine each protein's function in axon guidance and to dissect the signaling mechanisms that regulate the key effector activities. ..
  5. Mechanisms of Wound Healing in Drosophila
    Susan Parkhurst; Fiscal Year: 2007
    ..abstract_text> ..
  6. Mechanisms of Rho GTPase Function in Drosophila
    Susan Parkhurst; Fiscal Year: 2006
    ..Our results are expected to have wide implications as Rho GTPases and the diverse cellular mechanisms they mediate are conserved across a wide range of organisms. ..
  7. Genetic Analysis of Heavy Metals Neurotoxicity
    Fen Biao Gao; Fiscal Year: 2005
    ....
  8. Mechanisms of Roundabout Repulsive Axon Guidance
    GREG BASHAW; Fiscal Year: 2007
    ..Slit repulsion is mediated by the conserved family of Roundabout (Robo) receptors...
  9. Regulation of Cell Migration by the APC-Microtubule Complex
    W Nelson; Fiscal Year: 2007
    ....
  10. Mechanisms of Slit-Roundabout Mediated Axon Repulsion
    Greg J Bashaw; Fiscal Year: 2010
    ..A previously successful chimeric receptor approach where different portions of the Robo1, Robo2 and Robo3 receptors are exchanged with each other and then assayed for function in transgenic flies will be ..
  11. Transciption Factor Codes, Guidance Receptors and Motor Axon Guidance
    Greg J Bashaw; Fiscal Year: 2010
    ....
  12. Molecular Genetics of Stem Cell in Drosophila
    Krishna Moorthi Bhat; Fiscal Year: 2010
    ..These studies will help understand pathways that govern the self-renewing and terminal asymmetric division of precursor cells in multi-cellular organisms. ..
  13. Regulation of Cell Migration by the APC-Microtubule Complex
    William Nelson; Fiscal Year: 2009
    ..abstract_text> ..
  14. Genes Essential To Motor Axon Guidance in Drosophila
    David Van Vactor; Fiscal Year: 2009
    ..We will use a combination of genetics, cell biology and biochemistry to determine each protein's function in axon guidance and to dissect the signaling mechanisms that regulate the key effector activities. ..
  15. Transciption Factor Codes, Guidance Receptors and Motor Axon Guidance
    GREG BASHAW; Fiscal Year: 2009
    ....
  16. Cytoskeleton Coordination in Neuronal Morphogenesis
    W Nelson; Fiscal Year: 2005
    ....
  17. TOGENESIS OF NA+,K+-ATPASE IN POLARIZED MDCK EPITHELIAL
    W Nelson; Fiscal Year: 1993
    ....
  18. GENES ESSENTIAL TO MOTOR AXON GUIDANCE IN DROSOPHILIA
    David Van Vactor; Fiscal Year: 2000
    ..If it is required outside the motor neuron, it could be the still elusive ligand for DLAR. All of these studies will use techniques that are standard in the field and are feasible for this investigator. ..
  19. Mechanisms of Rho GTPase Function in Drosophila
    Susan Parkhurst; Fiscal Year: 2003
    ..Our results are expected to have wide implications as Rho GTPases and the diverse cellular mechanisms they mediate are conserved across a wide range of organisms. ..
  20. MECHANISMS OF TRANSCRIPTIONAL REPRESSION IN DROSOPHILA
    Susan Parkhurst; Fiscal Year: 2002
    ..Results obtained from these projects are expected to have wide implications as Hairy/E(spl) proteins and mechanisms of transcriptional repression are conserved throughout organisms. ..
  21. Animal Models For Studying Angelman Syndrome, A Developmental Brain Disorder
    Fen Biao Gao; Fiscal Year: 2007
    ..RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page J Description, ..
  22. GENES ESSENTIAL TO MOTOR AXON GUIDANCE IN DROSOPHILIA
    David Van Vactor; Fiscal Year: 2004
    ....