robl

Summary

Gene Symbol: robl
Description: roadblock
Alias: CG10751, DLC, Dmel\CG10751, LC7, l(2)k10408, roblk, roadblock, CG10751-PA, dynein light chain, dynein light chain LC7, dynein, light chain, roadblock-type 2, robl-PA
Species: fruit fly

Top Publications

  1. Reuter J, Nardine T, Penton A, Billuart P, Scott E, Usui T, et al. A mosaic genetic screen for genes necessary for Drosophila mushroom body neuronal morphogenesis. Development. 2003;130:1203-13 pubmed
    ..We found that roadblock, which encodes a dynein light chain, exhibits reduced cell number in neuroblast clones, reduced dendritic complexity and defective axonal ..
  2. Reis G, Yang G, Szpankowski L, Weaver C, Shah S, Robinson J, et al. Molecular motor function in axonal transport in vivo probed by genetic and computational analysis in Drosophila. Mol Biol Cell. 2012;23:1700-14 pubmed publisher
    ..Our data also suggest kinesin-1 and cytoplasmic dynein motors assemble in stable mixtures on APP vesicles and their direction and velocity are controlled at least in part by dynein intermediate chain. ..
  3. Susalka S, Nikulina K, Salata M, Vaughan P, King S, Vaughan K, et al. The roadblock light chain binds a novel region of the cytoplasmic Dynein intermediate chain. J Biol Chem. 2002;277:32939-46 pubmed
    ..It is composed of a number of different subunits including three light chain families: Tctex1, LC8, and roadblock. The incorporation of the roadblock light chains into the cytoplasmic dynein complex had not been determined...
  4. Bowman A, Patel King R, Benashski S, McCaffery J, Goldstein L, King S. Drosophila roadblock and Chlamydomonas LC7: a conserved family of dynein-associated proteins involved in axonal transport, flagellar motility, and mitosis. J Cell Biol. 1999;146:165-80 pubmed
    ..One of the mutants identified in this screen, roadblock (robl), exhibits diverse defects in intracellular transport including axonal transport and mitosis...
  5. Gunawardena S, Her L, Brusch R, Laymon R, Niesman I, Gordesky Gold B, et al. Disruption of axonal transport by loss of huntingtin or expression of pathogenic polyQ proteins in Drosophila. Neuron. 2003;40:25-40 pubmed
    ..Thus, disruption of axonal transport by pathogenic polyQ proteins could contribute to early neuropathology in Huntington's and other polyQ expansion diseases. ..
  6. Gunawardena S, Goldstein L. Disruption of axonal transport and neuronal viability by amyloid precursor protein mutations in Drosophila. Neuron. 2001;32:389-401 pubmed
    ..We discuss the possibility that axonal transport disruption may play a role in the neurodegenerative pathology of Alzheimer's disease. ..
  7. Iacobucci G, Rahman N, ValtueƱa A, Nayak T, Gunawardena S. Spatial and temporal characteristics of normal and perturbed vesicle transport. PLoS ONE. 2014;9:e97237 pubmed publisher
    ..Therefore evaluating the spatial and temporal characteristics of vesicle transport has important implications for our understanding of how transport defects can affect other pathways to initiate death and degeneration. ..
  8. Dix C, Soundararajan H, Dzhindzhev N, Begum F, Suter B, Ohkura H, et al. Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs. J Cell Biol. 2013;202:479-94 pubmed publisher
    ..Our data therefore reveal a critical role for Lis1 within the mRNA localization machinery and suggest a model in which Lis1 facilitates motor complex association with cargos by promoting the interaction of dynein with dynactin. ..
  9. Power D, Srinivasan S, Gunawardena S. In-vivo evidence for the disruption of Rab11 vesicle transport by loss of huntingtin. Neuroreport. 2012;23:970-7 pubmed publisher
    ..These results suggest that HTT plays a key role in the movement of Rab11 vesicles within axons. Thus, disruption of transport mediated by mutant HTT could contribute to early neuropathology observed in Huntington's diseases. ..

More Information

Publications17

  1. Nyarko A, Barbar E. Light chain-dependent self-association of dynein intermediate chain. J Biol Chem. 2011;286:1556-66 pubmed publisher
    ..However, recent studies show that the predicted coiled-coil sequence partially overlaps the light chain LC7 recognition sequence on IC, raising questions about the apparently contradictory effects of LC8 and LC7...
  2. Kang M, Hansen T, Mickiewicz M, Kaczynski T, Fye S, Gunawardena S. Disruption of axonal transport perturbs bone morphogenetic protein (BMP)--signaling and contributes to synaptic abnormalities in two neurodegenerative diseases. PLoS ONE. 2014;9:e104617 pubmed publisher
    ..Thus problems in axonal transport occurs early, perturbs BMP signaling, and likely contributes to the synaptic abnormalities observed in these two diseases...
  3. Copf T. Developmental shaping of dendritic arbors in Drosophila relies on tightly regulated intra-neuronal activity of protein kinase A (PKA). Dev Biol. 2014;393:282-297 pubmed publisher
    ..Our data argue that tightly controlled dose-dependent intra-neuronal PKA activity levels are critical in determining the dendritic arbor complexity, one of the possible ways being through the regulation of organelle distribution. ..
  4. Hall J, Song Y, Karplus P, Barbar E. The crystal structure of dynein intermediate chain-light chain roadblock complex gives new insights into dynein assembly. J Biol Chem. 2010;285:22566-75 pubmed publisher
    The roadblock/LC7 dynein light chain is a ubiquitous component of all dyneins and is essential for many diverse processes including proper axonal transport and dendrite growth...
  5. Hall J, Karplus P, Barbar E. Multivalency in the assembly of intrinsically disordered Dynein intermediate chain. J Biol Chem. 2009;284:33115-21 pubmed publisher
    ..Our results show assembled dynein IC as an elongated, flexible polybivalent duplex, and suggest that polybivalency is an important general mechanism for constructing stable yet reversible and functionally versatile complexes. ..
  6. Satoh D, Sato D, Tsuyama T, Saito M, Ohkura H, Rolls M, et al. Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes. Nat Cell Biol. 2008;10:1164-71 pubmed publisher
    ..These results reveal an important link between microtubule motors and endosomes in dendrite morphogenesis. ..
  7. Pfister K, Shah P, Hummerich H, Russ A, Cotton J, Annuar A, et al. Genetic analysis of the cytoplasmic dynein subunit families. PLoS Genet. 2006;2:e1 pubmed
  8. Gao F, Bogert B. Genetic control of dendritic morphogenesis in Drosophila. Trends Neurosci. 2003;26:262-8 pubmed