Gene Symbol: Rnp4F
Description: RNA-binding protein 4F
Alias: 4F-rnp, 4f-rnp, CG3312, Dmel\CG3312, RNP-4F, Rnp-4F, rnp-4f, RNA-binding protein 4F, CG3312-PA, CG3312-PB, CG3312-PD, CG3312-PF, Rnp4F-PA, Rnp4F-PB, Rnp4F-PD, Rnp4F-PF, splicing assembly factor rnp-4f
Species: fruit fly

Top Publications

  1. Petschek J, Mermer M, Scheckelhoff M, Simone A, Vaughn J. RNA editing in Drosophila 4f-rnp gene nuclear transcripts by multiple A-to-G conversions. J Mol Biol. 1996;259:885-90 pubmed
    ..The true significance of our discovery is that it was made in Drosophila, and the advantages of using Drosophila as a genetic model to further study RNA editing in the 4f-rnp gene are discussed. ..
  2. Fetherson R, Strock S, White K, Vaughn J. Alternative pre-mRNA splicing in Drosophila spliceosomal assembly factor RNP-4F during development. Gene. 2006;371:234-45 pubmed
    ..A model is proposed for the role of this element both in 5'-UTR alternative splicing decisions and in RNP-4F translational modulation. Preliminary evidences in support of our model are discussed. ..
  3. Chen J, Concel V, Bhatla S, Rajeshwaran R, Smith D, Varadarajan M, et al. Alternative splicing of an rnp-4f mRNA isoform retaining an evolutionarily-conserved 5'-UTR intronic element is developmentally regulated and shown via RNAi to be essential for normal central nervous system development in Drosophila melanogaster. Gene. 2007;399:91-104 pubmed
    ..Long rnp-4f mRNA knockdown via RNAi reveals a variety of CNS abnormalities, which leads us to postulate that this isoform acts upstream of other genes which have been shown to be important for normal CNS development. ..
  4. Peters N, Rohrbach J, Zalewski B, Byrkett C, Vaughn J. RNA editing and regulation of Drosophila 4f-rnp expression by sas-10 antisense readthrough mRNA transcripts. RNA. 2003;9:698-710 pubmed
    ..The results provide insight into roles of naturally occurring antisense RNAs in regulation of eukaryotic gene expression. ..
  5. Lakshmi G, Ghosh S, Jones G, Parikh R, Rawlins B, Vaughn J. An RNA electrophoretic mobility shift and mutational analysis of rnp-4f 5'-UTR intron splicing regulatory proteins in Drosophila reveals a novel new role for a dADAR protein isoform. Gene. 2012;511:161-8 pubmed publisher
    ..Taken together, these results demonstrate a novel role for dADAR protein in rnp-4f 5'-UTR alternative intron splicing regulation which is consistent with a previously proposed model. ..
  6. Ghosh S, Wang Y, Cook J, Chhiba L, Vaughn J. A molecular, phylogenetic and functional study of the dADAR mRNA truncated isoform during Drosophila embryonic development reveals an editing-independent function. Open J Anim Sci. 2013;3:20-30 pubmed
    ..These results demonstrate a novel, non-catalytic function for the truncated dADAR protein isoform in Drosophila embryonic development, which is very likely evolutionarily conserved. ..
  7. Hess K, Simone A, Petschek J. Spatial and temporal expression of 4f-rnp gene in Drosophila melanogaster. Differentiation. 1996;61:103-11 pubmed
    ..We discuss the significance that RNA editing is expected to have for the predicted 4F-RNP protein products that may be expressed throughout fly development and in high abundance within the fly's central nervous system. ..
  8. Chen J, Yang J, Doctor D, Rawlins B, Shields B, Vaughn J. 5'-UTR mediated translational control of splicing assembly factor RNP-4F expression during development of the Drosophila central nervous system. Gene. 2013;528:154-62 pubmed publisher
    ..The potential molecular mechanism behind the observed translational enhancement is discussed. ..
  9. Petschek J, Scheckelhoff M, Mermer M, Vaughn J. RNA editing and alternative splicing generate mRNA transcript diversity from the Drosophila 4f-rnp locus. Gene. 1997;204:267-76 pubmed

More Information


  1. Feiber A, Rangarajan J, Vaughn J. The evolution of single-copy Drosophila nuclear 4f-rnp genes: spliceosomal intron losses create polymorphic alleles. J Mol Evol. 2002;55:401-13 pubmed
    ..The results provide insights into the evolution of intron loss and may lead to improved understanding of the dynamics of this process in natural populations. ..
  2. Chen J, Lakshmi G, Hays D, McDowell K, Ma E, Vaughn J. Spatial and temporal expression of dADAR mRNA and protein isoforms during embryogenesis in Drosophila melanogaster. Differentiation. 2009;78:312-20 pubmed publisher
    ..In dADAR null mutants, rnp-4f 5'-UTR alternative splicing is significantly diminished, suggesting a non-catalytic role for dADAR in splicing regulation. A working model is proposed which provides a possible molecular mechanism. ..
  3. Keegan L, McGurk L, Palavicini J, Brindle J, Paro S, Li X, et al. Functional conservation in human and Drosophila of Metazoan ADAR2 involved in RNA editing: loss of ADAR1 in insects. Nucleic Acids Res. 2011;39:7249-62 pubmed publisher
    ..The ADAR1 gene has been lost several times, including during the evolution of insects and crustacea. These data complement our rescue results, supporting the idea that ADAR1 and ADAR2 have evolved highly conserved, distinct functions. ..
  4. Lavorgna G, Dahary D, Lehner B, Sorek R, Sanderson C, Casari G. In search of antisense. Trends Biochem Sci. 2004;29:88-94 pubmed
    ..A range of bioinformatic resources could be used to assist future antisense research. ..
  5. Vaughn J, Ghosh S, Chen J. A phylogenetic study of Drosophila splicing assembly chaperone RNP-4F associated U4-/U6-snRNA secondary structure. Open J Anim Sci. 2013;3:36-48 pubmed