Rho1

Summary

Gene Symbol: Rho1
Description: Rho1
Alias: 19549712, 8416, AAF01186, CG8416, D-Rho1, DRho, DRho1, DRhoA, Dm Rho1, DmRHO-A, Dmel\CG8416, Drho1, DrhoA, E3.10/J3.8, P48148, RHO, RHO1, Rho, Rho A, Rho GTPase, Rho kinase, RhoA, RhoN19, dRho1, dRhoA, dnRho, l(2)52Fa, l(2)k02107b, n(2)k07236, rho, rho1, rhoA, Rho1, CG8416-PA, CG8416-PB, CG8416-PC, CG8416-PD, CG8416-PF, CG8416-PG, Rho GTPase, Rho1-PA, Rho1-PB, Rho1-PC, Rho1-PD, Rho1-PF, Rho1-PG, RhoA, RhoA kinase, rho kinase, rho1, rho1 kinase
Species: fruit fly
Products:     Rho1

Top Publications

  1. Halsell S, Chu B, Kiehart D. Genetic analysis demonstrates a direct link between rho signaling and nonmuscle myosin function during Drosophila morphogenesis. Genetics. 2000;155:1253-65 pubmed
    ..we show that two EMS-induced mutations, previously shown to interact genetically with zipper(Ebr), disrupt the RhoA locus...
  2. Warner S, Longmore G. Distinct functions for Rho1 in maintaining adherens junctions and apical tension in remodeling epithelia. J Cell Biol. 2009;185:1111-25 pubmed publisher
    ..Using clonal analysis in the postmitotic Drosophila melanogaster pupal eye epithelium, we demonstrate that Rho1 is required to maintain AJ integrity independent of its role in sustaining apical cell tension...
  3. Lee D, Harris T. An Arf-GEF regulates antagonism between endocytosis and the cytoskeleton for Drosophila blastoderm development. Curr Biol. 2013;23:2110-20 pubmed publisher
    ..Endocytosis also regulates the PM and can be promoted or inhibited by cytoskeletal networks. However, endocytic regulation of the general membrane cytoskeleton is undocumented...
  4. Chen G, Turano B, Ruest P, Hagel M, Settleman J, Thomas S. Regulation of Rho and Rac signaling to the actin cytoskeleton by paxillin during Drosophila development. Mol Cell Biol. 2005;25:979-87 pubmed
    ..These defects may reflect a function for paxillin in regulation of Rho family GTPase signaling as paxillin interacts genetically with Rac and Rho in the developing eye...
  5. Zimmerman S, Thorpe L, Medrano V, Mallozzi C, McCartney B. Apical constriction and invagination downstream of the canonical Wnt signaling pathway require Rho1 and Myosin II. Dev Biol. 2010;340:54-66 pubmed publisher
    ..Further, we show that disruption of Rho1 suppresses apical constriction and invagination in APC null cells...
  6. Baek S, Kwon Y, Lee H, Choe K. Rho-family small GTPases are required for cell polarization and directional sensing in Drosophila wound healing. Biochem Biophys Res Commun. 2010;394:488-92 pubmed publisher
    ..Here, we use myosin localization to demonstrate that Rac1, Cdc42, and Rho1 are each required for cell polarization and directional sensing of the wound...
  7. Kadandale P, Stender J, Glass C, Kiger A. Conserved role for autophagy in Rho1-mediated cortical remodeling and blood cell recruitment. Proc Natl Acad Sci U S A. 2010;107:10502-7 pubmed publisher
    ..Cortical remodeling is achieved under the central control of Rho GTPase pathways that modulate an exquisite balance in the dynamic assembly and disassembly of the cytoskeleton and ..
  8. Somma M, Fasulo B, Cenci G, Cundari E, Gatti M. Molecular dissection of cytokinesis by RNA interference in Drosophila cultured cells. Mol Biol Cell. 2002;13:2448-60 pubmed
    ..We show that double-stranded RNAs for anillin, acGAP, pavarotti, rho1, pebble, spaghetti squash, syntaxin1A, and twinstar all disrupt cytokinesis in S2 tissue culture cells, causing ..
  9. Johnson R, Seppa M, Cagan R. The Drosophila CD2AP/CIN85 orthologue Cindr regulates junctions and cytoskeleton dynamics during tissue patterning. J Cell Biol. 2008;180:1191-204 pubmed publisher
    ..Together, these data demonstrate that Cindr is involved in dynamic cell rearrangement in an emerging epithelium. ..

More Information

Publications94

  1. Brodu V, Casanova J. The RhoGAP crossveinless-c links trachealess and EGFR signaling to cell shape remodeling in Drosophila tracheal invagination. Genes Dev. 2006;20:1817-28 pubmed
    ..Our results identify a developmental pathway linking cell fate genes and cell signaling pathways to intracellular modifications during tracheal cell invagination. ..
  2. Strutt D, Weber U, Mlodzik M. The role of RhoA in tissue polarity and Frizzled signalling. Nature. 1997;387:292-5 pubmed
    ..We have identified mutations in the Drosophila homologue of RhoA p21 GTPase, and by analysis of their phenotype show that RhoA is required for the generation of tissue polarity...
  3. Hariharan I, Hu K, Asha H, Quintanilla A, Ezzell R, Settleman J. Characterization of rho GTPase family homologues in Drosophila melanogaster: overexpressing Rho1 in retinal cells causes a late developmental defect. EMBO J. 1995;14:292-302 pubmed
    ..We have cloned three widely expressed members of this family from Drosophila melanogaster; a rho homologue (Rho1) and two rac homologues (Rac1 and Rac2)...
  4. Stramer B, Wood W, Galko M, Redd M, Jacinto A, Parkhurst S, et al. Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration. J Cell Biol. 2005;168:567-73 pubmed
    ..For the first time, we are able to examine the roles of Rho-family small GTPases during inflammation in vivo and show that Rac-mediated lamellae are essential for hemocyte ..
  5. Song J, Giniger E. Noncanonical Notch function in motor axon guidance is mediated by Rac GTPase and the GEF1 domain of Trio. Dev Dyn. 2011;240:324-32 pubmed publisher
    ..We infer, therefore, that Rac is a key component of Abl signaling in Drosophila motor axons, and specifically that it is the crucial Rho GTPase in "noncanonical" Notch/Abl signaling.
  6. Dean S, Spudich J. Rho kinase's role in myosin recruitment to the equatorial cortex of mitotic Drosophila S2 cells is for myosin regulatory light chain phosphorylation. PLoS ONE. 2006;1:e131 pubmed
    ..S2 cells, we previously showed that myosin II is recruited to the furrow independently of F-actin, and that Rho1 and Rok are essential for this recruitment [1]...
  7. Colinet D, Schmitz A, Depoix D, Crochard D, Poirié M. Convergent use of RhoGAP toxins by eukaryotic parasites and bacterial pathogens. PLoS Pathog. 2007;3:e203 pubmed publisher
    ..Some bacterial toxins mimic eukaryotic Rho GTPase-activating proteins (GAPs) to inactivate mammalian GTPases, probably as a result of evolutionary convergence...
  8. Dawes Hoang R, Parmar K, Christiansen A, Phelps C, Brand A, Wieschaus E. folded gastrulation, cell shape change and the control of myosin localization. Development. 2005;132:4165-78 pubmed
    ..We determine that this contractility driven form of localization involves RhoGEF2 and the downstream effector Rho kinase. This distinguishes apical myosin localization from basal myosin localization, which we find not to require ..
  9. Delalle I, Pfleger C, Buff E, Lueras P, Hariharan I. Mutations in the Drosophila orthologs of the F-actin capping protein alpha- and beta-subunits cause actin accumulation and subsequent retinal degeneration. Genetics. 2005;171:1757-65 pubmed
    ..In its absence, cells progressively accumulate actin filaments and eventually die. A possible role for their human orthologs in neurodegenerative disease merits further investigation. ..
  10. Simoes S, Denholm B, Azevedo D, Sotillos S, Martin P, Skaer H, et al. Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis. Development. 2006;133:4257-67 pubmed
    ..of a new probe that allows the visualisation of small RhoGTPase activity in Drosophila, we present evidence that Rho1 is apically activated and essential for epithelial cell invagination, a common morphogenetic movement during ..
  11. Emery G, Hutterer A, Berdnik D, Mayer B, Wirtz Peitz F, Gaitan M, et al. Asymmetric Rab 11 endosomes regulate delta recycling and specify cell fate in the Drosophila nervous system. Cell. 2005;122:763-73 pubmed
    ..Our results suggest that cells can regulate signaling pathways and influence their developmental fate by inhibiting the formation of individual endocytic compartments...
  12. Nahm M, Long A, Paik S, Kim S, Bae Y, Broadie K, et al. The Cdc42-selective GAP rich regulates postsynaptic development and retrograde BMP transsynaptic signaling. J Cell Biol. 2010;191:661-75 pubmed publisher
    ..Importantly, dRich increases Gbb release and elevates presynaptic phosphorylated Mad levels. We propose that dRich coordinates the Gbb-dependent modulation of synaptic growth and function with postsynaptic development. ..
  13. Brumby A, Richardson H. scribble mutants cooperate with oncogenic Ras or Notch to cause neoplastic overgrowth in Drosophila. EMBO J. 2003;22:5769-79 pubmed
  14. Shandala T, Gregory S, Dalton H, Smallhorn M, Saint R. Citron kinase is an essential effector of the Pbl-activated Rho signalling pathway in Drosophila melanogaster. Development. 2004;131:5053-63 pubmed
    Pebble (Pbl)-activated RhoA signalling is essential for cytokinesis in Drosophila melanogaster. Here we report that the Drosophila citron gene encodes an essential effector kinase of Pbl-RhoA signalling in vivo...
  15. Ball R, Warren Paquin M, Tsurudome K, Liao E, Elazzouzi F, Cavanagh C, et al. Retrograde BMP signaling controls synaptic growth at the NMJ by regulating trio expression in motor neurons. Neuron. 2010;66:536-49 pubmed publisher
    ..Based on our findings, we propose a model in which a retrograde BMP signal from the muscle modulates GTPase activity through transcriptional regulation of Rac GEF trio, thereby regulating the homeostasis of synaptic growth at the NMJ. ..
  16. Boutros M, Paricio N, Strutt D, Mlodzik M. Dishevelled activates JNK and discriminates between JNK pathways in planar polarity and wingless signaling. Cell. 1998;94:109-18 pubmed
    ..Our results indicate that different Wnt/Fz signals activate distinct intracellular pathways, and Dishevelled discriminates among them by distinct domain interactions. ..
  17. Cao J, Albertson R, Riggs B, Field C, Sullivan W. Nuf, a Rab11 effector, maintains cytokinetic furrow integrity by promoting local actin polymerization. J Cell Biol. 2008;182:301-13 pubmed publisher
    ..We also find that RhoGEF2 does not properly localize at the furrow in nuf mutant embryos and that RhoGEF2-Rho1 pathway components show strong specific genetic interactions with Nuf...
  18. Lee T, Winter C, Marticke S, Lee A, Luo L. Essential roles of Drosophila RhoA in the regulation of neuroblast proliferation and dendritic but not axonal morphogenesis. Neuron. 2000;25:307-16 pubmed
    ..report here the use of the MARCM (mosaic analysis with a repressible cell marker) system to analyze the function of RhoA in the developing Drosophila brain...
  19. Ben El Kadhi K, Roubinet C, Solinet S, Emery G, Carreno S. The inositol 5-phosphatase dOCRL controls PI(4,5)P2 homeostasis and is necessary for cytokinesis. Curr Biol. 2011;21:1074-9 pubmed publisher
    ..Identification of dOCRL as essential for cell division may be important to understand the molecular basis of the phenotypic manifestations of Lowe syndrome. ..
  20. Sato D, Sugimura K, Satoh D, Uemura T. Crossveinless-c, the Drosophila homolog of tumor suppressor DLC1, regulates directional elongation of dendritic branches via down-regulating Rho1 activity. Genes Cells. 2010;15:485-500 pubmed publisher
    ..Cv-c belongs to the family of Rho GTPase-activating proteins (RhoGAPs) and is the homolog of human tumor suppressor DLC1...
  21. Xu N, Bagumian G, Galiano M, Myat M. Rho GTPase controls Drosophila salivary gland lumen size through regulation of the actin cytoskeleton and Moesin. Development. 2011;138:5415-27 pubmed publisher
    ..actin polymerization and regulating F-actin distribution at the apical and basolateral membranes through Rho kinase. Loss of Rho1 resulted in reduction of F-actin at the basolateral membrane and enrichment of apical F-actin, the ..
  22. Mizuno T, Tsutsui K, Nishida Y. Drosophila myosin phosphatase and its role in dorsal closure. Development. 2002;129:1215-23 pubmed
    ..Similar defects were seen in embryos overexpressing Rho-kinase, suggesting that myosin phosphatase and Rho-kinase function antagonistically...
  23. Winter C, Wang B, Ballew A, Royou A, Karess R, Axelrod J, et al. Drosophila Rho-associated kinase (Drok) links Frizzled-mediated planar cell polarity signaling to the actin cytoskeleton. Cell. 2001;105:81-91 pubmed
    ..Here, we show that Drosophila Rho-associated kinase (Drok) works downstream of Fz/Dsh to mediate a branch of the planar polarity pathway involved in ..
  24. Fox D, Homem C, Myster S, Wang F, Bain E, Peifer M. Rho1 regulates Drosophila adherens junctions independently of p120ctn. Development. 2005;132:4819-31 pubmed
    ..Further, Rho1 localization is not obviously altered by loss of p120 or by reduction of core AJ proteins...
  25. Hacker U, Perrimon N. DRhoGEF2 encodes a member of the Dbl family of oncogenes and controls cell shape changes during gastrulation in Drosophila. Genes Dev. 1998;12:274-84 pubmed
    ..Expression of dominant-negative DRhoA during gastrulation results in phenocopies of the DRhoGEF2 mutant, suggesting that a signaling cascade involving ..
  26. Xu N, Keung B, Myat M. Rho GTPase controls invagination and cohesive migration of the Drosophila salivary gland through Crumbs and Rho-kinase. Dev Biol. 2008;321:88-100 pubmed publisher
    ..have been shown to be essential for epithelial morphogenesis in cell culture; however, the mechanism by which Rho GTPase and its downstream effectors control coordinated movement of epithelia in a developing tissue or organ is ..
  27. Govek E, Newey S, Van Aelst L. The role of the Rho GTPases in neuronal development. Genes Dev. 2005;19:1-49 pubmed
    ..This review will focus on the role of the Rho GTPases and their associated signaling molecules throughout neuronal development and discuss how perturbations in Rho GTPase signaling may lead to cognitive disorders.
  28. Cook M, Mani P, Wentzell J, Kretzschmar D. Increased RhoA prenylation in the loechrig (loe) mutant leads to progressive neurodegeneration. PLoS ONE. 2012;7:e44440 pubmed publisher
    ..that the loe mutation interferes with isoprenoid synthesis, leading to increased prenylation of the small GTPase Rho1, the fly orthologue of vertebrate RhoA...
  29. Magie C, Meyer M, Gorsuch M, Parkhurst S. Mutations in the Rho1 small GTPase disrupt morphogenesis and segmentation during early Drosophila development. Development. 1999;126:5353-64 pubmed
    ..We isolated loss-of-function mutations in the Drosophila Rho1 (Rho1) gene during a genetic screen for maternal-effect mutations, allowing us to investigate the specific roles ..
  30. Newsome T, Schmidt S, Dietzl G, Keleman K, Asling B, Debant A, et al. Trio combines with dock to regulate Pak activity during photoreceptor axon pathfinding in Drosophila. Cell. 2000;101:283-94 pubmed
    ..We propose that distinct signals transduced via Trio and Dock act combinatorially to activate Pak in spatially restricted domains within the growth cone, thereby controlling the direction of axon extension. ..
  31. Lee S, Kolodziej P. The plakin Short Stop and the RhoA GTPase are required for E-cadherin-dependent apical surface remodeling during tracheal tube fusion. Development. 2002;129:1509-20 pubmed
    ..Expression of constitutively active RhoA in all tracheal cells mimics the shot phenotype and affects Shot localization in fusion cells...
  32. Muyskens J, Guillemin K. Helicobacter pylori CagA disrupts epithelial patterning by activating myosin light chain. PLoS ONE. 2011;6:e17856 pubmed publisher
    ..Therefore, in our transgenic system, CagA is targeted by EPIYA motifs to a specific apical region of the epithelium where it efficiently activates MLC to disrupt epithelial integrity. ..
  33. Simões S, Blankenship J, Weitz O, Farrell D, Tamada M, Fernandez Gonzalez R, et al. Rho-kinase directs Bazooka/Par-3 planar polarity during Drosophila axis elongation. Dev Cell. 2010;19:377-88 pubmed publisher
    ..The myosin II activator Rho-kinase is asymmetrically enriched at the anterior and posterior borders of intercalating cells in a pattern ..
  34. Bayer C, Halsell S, Fristrom J, Kiehart D, von Kalm L. Genetic interactions between the RhoA and Stubble-stubbloid loci suggest a role for a type II transmembrane serine protease in intracellular signaling during Drosophila imaginal disc morphogenesis. Genetics. 2003;165:1417-32 pubmed
    The Drosophila RhoA (Rho1) GTPase is essential for postembryonic morphogenesis of leg and wing imaginal discs...
  35. Halsell S, Kiehart D. Second-site noncomplementation identifies genomic regions required for Drosophila nonmuscle myosin function during morphogenesis. Genetics. 1998;148:1845-63 pubmed
    ..10/J3.8, for leg morphogenesis and viability. ..
  36. Vereshchagina N, Bennett D, Szoor B, Kirchner J, Gross S, Vissi E, et al. The essential role of PP1beta in Drosophila is to regulate nonmuscle myosin. Mol Biol Cell. 2004;15:4395-405 pubmed
    ..The similarity between fly and human PP1beta and MYPT genes suggests this role may be conserved. ..
  37. Carreno S, Kouranti I, Glusman E, Fuller M, Echard A, Payre F. Moesin and its activating kinase Slik are required for cortical stability and microtubule organization in mitotic cells. J Cell Biol. 2008;180:739-46 pubmed publisher
    ..We propose that the spatiotemporal control of moesin activation at the mitotic cortex provides localized cues to coordinate cortical contractility and microtubule interactions during cell division. ..
  38. Uehara R, Goshima G, Mabuchi I, Vale R, Spudich J, Griffis E. Determinants of myosin II cortical localization during cytokinesis. Curr Biol. 2010;20:1080-5 pubmed publisher
    ..Surprisingly, the third event can occur to a degree in a Rho-independent fashion, gathering preassembled filaments to the equatorial zone via cortical flow...
  39. Wood W, Jacinto A, Grose R, Woolner S, Gale J, Wilson C, et al. Wound healing recapitulates morphogenesis in Drosophila embryos. Nat Cell Biol. 2002;4:907-12 pubmed
    ..Modulating the activities of the small GTPases Rho and Cdc42 demonstrates that these actin-dependent elements have differing cellular functions, but that either alone ..
  40. Montell D. The genetics of cell migration in Drosophila melanogaster and Caenorhabditis elegans development. Development. 1999;126:3035-46 pubmed
    ..link between membrane receptor signaling and regulation of the actin cytoskeleton is that provided by the Rho family of small GTPases...
  41. Hipfner D, Keller N, Cohen S. Slik Sterile-20 kinase regulates Moesin activity to promote epithelial integrity during tissue growth. Genes Dev. 2004;18:2243-8 pubmed
    ..behavior of slik mutant cells is due to failure to phosphorylate and activate Moesin, which leads to excess Rho1 activity. This is distinct from Slik's effects on cell proliferation, which are mediated by Raf...
  42. Williams M, Habayeb M, Hultmark D. Reciprocal regulation of Rac1 and Rho1 in Drosophila circulating immune surveillance cells. J Cell Sci. 2007;120:502-11 pubmed
    ..To further elucidate this interaction we examined the activities of Rac1 and Rho1 in Drosophila cellular immune surveillance cells...
  43. Rogers S, Wiedemann U, Hacker U, Turck C, Vale R. Drosophila RhoGEF2 associates with microtubule plus ends in an EB1-dependent manner. Curr Biol. 2004;14:1827-33 pubmed
    ..as a model system, we show that DRhoGEF2 induces contractile cell shape changes by stimulating myosin II via the Rho1 pathway...
  44. Greco V, Hannus M, Eaton S. Argosomes: a potential vehicle for the spread of morphogens through epithelia. Cell. 2001;106:633-45 pubmed
    ..Wingless protein colocalizes with argosomes derived from Wingless-producing cells. The properties of argosomes are consistent with their being a vehicle for the spread of Wingless protein. ..
  45. Prokopenko S, Saint R, Bellen H. Untying the Gordian knot of cytokinesis. Role of small G proteins and their regulators. J Cell Biol. 2000;148:843-8 pubmed
  46. Dean S, Rogers S, Stuurman N, Vale R, Spudich J. Distinct pathways control recruitment and maintenance of myosin II at the cleavage furrow during cytokinesis. Proc Natl Acad Sci U S A. 2005;102:13473-8 pubmed
    ..Three genes in the rho1 signaling pathway, pebble (a Rho guanidine nucleotide exchange factor), rho1, and rho kinase, are required for the initial recruitment of myosin II to the equatorial cortex...
  47. Somers W, Saint R. A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis. Dev Cell. 2003;4:29-39 pubmed
    ..We show that an interaction between the Rho GTP exchange factor, Pebble, and the Rho family GTPase-activating protein, RacGAP50C, connects the contractile ring ..
  48. Grzeschik N, Knust E. IrreC/rst-mediated cell sorting during Drosophila pupal eye development depends on proper localisation of DE-cadherin. Development. 2005;132:2035-45 pubmed
    ..overexpression of DE-cadherin, the intracellular domain of Crumbs, or by a dominant version of the monomeric GTPase Rho1 prevents localisation of the transmembrane protein IrreC-rst to the border between primary pigment cells and IOCs...
  49. He L, Wang X, Tang H, Montell D. Tissue elongation requires oscillating contractions of a basal actomyosin network. Nat Cell Biol. 2010;12:1133-42 pubmed publisher
    ..Myosin accumulated in a periodic manner before each contraction and was regulated by the small GTPase Rho, its downstream kinase, ROCK, and cytosolic calcium...
  50. Goldbach P, Wong R, Beise N, Sarpal R, Trimble W, Brill J. Stabilization of the actomyosin ring enables spermatocyte cytokinesis in Drosophila. Mol Biol Cell. 2010;21:1482-93 pubmed publisher
    ..Our results show that the anillin-septin and cadherin-catenin complexes can serve as alternative cassettes to promote tight physical coupling of F-actin and myosin II to the cleavage furrow and successful completion of cytokinesis. ..
  51. Lu Y, Settleman J. The Drosophila Pkn protein kinase is a Rho/Rac effector target required for dorsal closure during embryogenesis. Genes Dev. 1999;13:1168-80 pubmed
    The PKN family of PKC-related protein kinases constitutes the major Rho GTPase-associated protein kinase activities detected in mammalian tissues. However, the biological functions of these kinases are unknown...
  52. Shulman J, Perrimon N, Axelrod J. Frizzled signaling and the developmental control of cell polarity. Trends Genet. 1998;14:452-8 pubmed
    ..Recently described examples from the nematode and frog suggest that the developmental control of cell polarity by FZ receptors might represent a functionally conserved signaling mechanism. ..
  53. Bloor J, Kiehart D. Drosophila RhoA regulates the cytoskeleton and cell-cell adhesion in the developing epidermis. Development. 2002;129:3173-83 pubmed
    ..By expressing the dominant negative DRhoA(N19) construct in stripes of epidermal cells, we confirm that Rho function is required for dorsal closure and ..
  54. Brumby A, Goulding K, Schlosser T, Loi S, Galea R, Khoo P, et al. Identification of novel Ras-cooperating oncogenes in Drosophila melanogaster: a RhoGEF/Rho-family/JNK pathway is a central driver of tumorigenesis. Genetics. 2011;188:105-25 pubmed publisher
    ..Ras(ACT)-cooperating genes identified were Rac1 Rho1, RhoGEF2, pbl, rib, and east, which encode cell morphology regulators...
  55. Ng J, Luo L. Rho GTPases regulate axon growth through convergent and divergent signaling pathways. Neuron. 2004;44:779-93 pubmed
    b>Rho GTPases are essential regulators of cytoskeletal reorganization, but how they do so during neuronal morphogenesis in vivo is poorly understood...
  56. Nahm M, Lee M, Baek S, Yoon J, Kim H, Lee Z, et al. Drosophila RhoGEF4 encodes a novel RhoA-specific guanine exchange factor that is highly expressed in the embryonic central nervous system. Gene. 2006;384:139-44 pubmed
    ..In this study, the DH-PH motif is shown to enhance the dissociation of GDP from either RhoA or Rac1 but not from Cdc42 in vitro...
  57. Grosshans J, Wenzl C, Herz H, Bartoszewski S, Schnorrer F, Vogt N, et al. RhoGEF2 and the formin Dia control the formation of the furrow canal by directed actin assembly during Drosophila cellularisation. Development. 2005;132:1009-20 pubmed
    ..Biochemical analysis shows that Rho1 interacts with both RhoGEF2 and Dia, and that Dia nucleates actin filaments...
  58. Bökel C, Brown N. Integrins in development: moving on, responding to, and sticking to the extracellular matrix. Dev Cell. 2002;3:311-21 pubmed
    ..Integrins also participate in signaling events that govern tissue differentiation and organogenesis. Finally, adhesion by integrin-mediated junctions allows tissues to withstand mechanical load and is essential for tissue integrity. ..
  59. Chen G, Gajowniczek P, Settleman J. Rho-LIM kinase signaling regulates ecdysone-induced gene expression and morphogenesis during Drosophila metamorphosis. Curr Biol. 2004;14:309-13 pubmed
    ..The Rho GTPase, which mediates cell shape change and migration, is also an essential regulator of tissue morphogenesis during ..
  60. Mirkovic I, Mlodzik M. Cooperative activities of drosophila DE-cadherin and DN-cadherin regulate the cell motility process of ommatidial rotation. Development. 2006;133:3283-93 pubmed
    ..A similar input into mammalian E- and N-cadherins might function in the progression of diseases such as metastatic ovarian cancer. ..
  61. Massarwa R, Schejter E, Shilo B. Apical secretion in epithelial tubes of the Drosophila embryo is directed by the Formin-family protein Diaphanous. Dev Cell. 2009;16:877-88 pubmed publisher
    ..is apically localized both at the RNA and protein levels, and apical localization of its activators, including Rho1 and two guanine exchange factor proteins (Rho-GEFs), contributes to its activity...
  62. Yan J, Lu Q, Fang X, Adler P. Rho1 has multiple functions in Drosophila wing planar polarity. Dev Biol. 2009;333:186-99 pubmed publisher
    ..Previous data implicated Rho1 as a component of the fz pathway in Drosophila but it was unclear how it functioned...
  63. Barros C, Phelps C, Brand A. Drosophila nonmuscle myosin II promotes the asymmetric segregation of cell fate determinants by cortical exclusion rather than active transport. Dev Cell. 2003;5:829-40 pubmed
    ..Myosin II is activated by Rho kinase and restricted to the apical cortex by the tumor suppressor Lethal (2) giant larvae...
  64. Neisch A, Speck O, Stronach B, Fehon R. Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane. J Cell Biol. 2010;189:311-23 pubmed publisher
    ..We demonstrate that the Drosophila melanogaster RhoA, Rho1, promotes apoptosis independently of Rho kinase through its effects on c-Jun NH(2)-terminal kinase (JNK) signaling...
  65. Jacinto A, Wood W, Woolner S, Hiley C, Turner L, Wilson C, et al. Dynamic analysis of actin cable function during Drosophila dorsal closure. Curr Biol. 2002;12:1245-50 pubmed
    ..of this cable by live analysis of GFP-actin-expressing embryos in which the cable is disrupted by modulating Rho1 signaling or by loss of non-muscle myosin (Zipper) function...
  66. Deng W, Schneider M, Frock R, Castillejo Lopez C, Gaman E, Baumgartner S, et al. Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila. Development. 2003;130:173-84 pubmed
    ..These data suggest that the primary function of Dystroglycan in oogenesis is to organize cellular polarity; and this study sets the stage for analyzing the Dystroglycan complex by using the power of Drosophila molecular genetics. ..
  67. Speck O, Hughes S, Noren N, Kulikauskas R, Fehon R. Moesin functions antagonistically to the Rho pathway to maintain epithelial integrity. Nature. 2003;421:83-7 pubmed
    ..not by providing an essential structural function, but rather by antagonizing activity of the small GTPase Rho. Thus, Moesin functions in maintaining epithelial integrity by regulating cell-signalling events that affect actin ..
  68. Mizuno T, Amano M, Kaibuchi K, Nishida Y. Identification and characterization of Drosophila homolog of Rho-kinase. Gene. 1999;238:437-44 pubmed
    ..A two-hybrid analysis demonstrated that DRho-kinase interacts with the GTP-bound form of the Drosophila Rho. Drho1, at the conserved Rho-binding site...
  69. Matusek T, Djiane A, Jankovics F, Brunner D, Mlodzik M, Mihaly J. The Drosophila formin DAAM regulates the tracheal cuticle pattern through organizing the actin cytoskeleton. Development. 2006;133:957-66 pubmed
    ..Our results indicate that although DAAM is regulated by RhoA, it functions upstream or parallel to the non-receptor tyrosine kinases Src42A and Tec29 to organize the actin ..
  70. Polaski S, Whitney L, Barker B, Stronach B. Genetic analysis of slipper/mixed lineage kinase reveals requirements in multiple Jun-N-terminal kinase-dependent morphogenetic events during Drosophila development. Genetics. 2006;174:719-33 pubmed
    ..Finally, we demonstrate that SLPR overexpression can induce ectopic JNK signaling and that the SLPR protein is enriched at the epithelial cell cortex. ..
  71. Vidal M, Larson D, Cagan R. Csk-deficient boundary cells are eliminated from normal Drosophila epithelia by exclusion, migration, and apoptosis. Dev Cell. 2006;10:33-44 pubmed
    ..These latter phenotypes required sharp differences in dCsk activity between neighbors; dE-cadherin, P120-catenin, Rho1, JNK, and MMP2 mediated this signal...
  72. D Avino P, Savoian M, Glover D. Cleavage furrow formation and ingression during animal cytokinesis: a microtubule legacy. J Cell Sci. 2005;118:1549-58 pubmed
    ..The same mechanism might be conserved in other organisms. ..
  73. Hu H, Li M, Labrador J, McEwen J, Lai E, Goodman C, et al. Cross GTPase-activating protein (CrossGAP)/Vilse links the Roundabout receptor to Rac to regulate midline repulsion. Proc Natl Acad Sci U S A. 2005;102:4613-8 pubmed
    The regulators of the Rho-family GTPases, GTPase-activating proteins (GAPs) and guanine exchange factors (GEFs), play important roles in axon guidance...
  74. Sanny J, Chui V, Langmann C, Pereira C, Zahedi B, Harden N. Drosophila RhoGAP68F is a putative GTPase activating protein for RhoA participating in gastrulation. Dev Genes Evol. 2006;216:543-50 pubmed
    ..despite the strong similarities between RhoGAP68F and p50RhoGAP/Cdc42GAP, RhoGAP68F is most effective as a GAP for RhoA. These in vitro data are supported by the in vivo analysis of mutants in RhoGAP68F...
  75. Noselli S. JNK signaling and morphogenesis in Drosophila. Trends Genet. 1998;14:33-8 pubmed
  76. Chen W, White M, Cobb M. Stimulus-specific requirements for MAP3 kinases in activating the JNK pathway. J Biol Chem. 2002;277:49105-10 pubmed
    ..On the other hand, sorbitol requires expression of four MAP3Ks to cause maximal JNK activation. Thus, we demonstrate that specific stimuli use different mechanisms to recruit distinct MAP3Ks to regulate the JNK pathway. ..
  77. Warrington S, Strutt H, Strutt D. The Frizzled-dependent planar polarity pathway locally promotes E-cadherin turnover via recruitment of RhoGEF2. Development. 2013;140:1045-54 pubmed publisher
    ..We thus reveal a general mechanism by which the core planar polarity pathway can promote polarised cell rearrangements. ..
  78. Denholm B, Brown S, Ray R, Ruiz Gomez M, Skaer H, Hombria J. crossveinless-c is a RhoGAP required for actin reorganisation during morphogenesis. Development. 2005;132:2389-400 pubmed
    ..Genetic interactions between cv-c and RhoGTPase mutants indicate that Rho1, Rac1 and Rac2 are substrates for Cv-c, and suggest that the substrate specificity might be regulated in a tissue-..
  79. Padash Barmchi M, Rogers S, Hacker U. DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo. J Cell Biol. 2005;168:575-85 pubmed
    ..Often, Rho1 controls different aspects of cytoskeletal function in parallel, requiring a complex level of regulation...
  80. Tan C, Stronach B, Perrimon N. Roles of myosin phosphatase during Drosophila development. Development. 2003;130:671-81 pubmed
    ..Our results indicate that the regulation of the phosphorylation of myosin regulatory light chains, or dynamic activation and inactivation of myosin II, is essential for its various functions during many developmental processes. ..
  81. Eaton S. Planar polarization of Drosophila and vertebrate epithelia. Curr Opin Cell Biol. 1997;9:860-6 pubmed
    ..Three different Rho family proteins have been shown to mediate polarization in the wing and the eye of Drosophila...
  82. Blanchard G, Murugesu S, Adams R, Martinez Arias A, Gorfinkiel N. Cytoskeletal dynamics and supracellular organisation of cell shape fluctuations during dorsal closure. Development. 2010;137:2743-52 pubmed publisher
    ..Our results suggest that cell shape fluctuations are a property of cells with low and increasing levels of apical myosin, and that medial and junctional myosin populations combine to contract AS cell apices and drive DC. ..
  83. Homem C, Peifer M. Exploring the roles of diaphanous and enabled activity in shaping the balance between filopodia and lamellipodia. Mol Biol Cell. 2009;20:5138-55 pubmed publisher
    ..Our data suggest they work together in a complex and nonadditive way, with the ratio between active Dia and Ena being one factor that modulates the balance between filopodia and lamellipodia. ..
  84. Albertson R, Cao J, Hsieh T, Sullivan W. Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle. J Cell Biol. 2008;181:777-90 pubmed publisher
    ..We find that Rho-specific guanine nucleotide exchange factor mutants, pebble (pbl), severely disrupt this F-actin-associated vesicle ..
  85. Abreu Blanco M, Verboon J, Parkhurst S. Coordination of Rho family GTPase activities to orchestrate cytoskeleton responses during cell wound repair. Curr Biol. 2014;24:144-55 pubmed publisher
    ..Genetic and pharmacological assays show that each GTPase makes specific contributions to the repair process. Rho1 is necessary for myosin II activation, leading to its association with actin...