Gene Symbol: Ote
Description: Otefin
Alias: CG5581, Dmel\CG5581, hal, ote, otefin, otefin, CG5581-PA, Ote-PA, halted, haulted
Species: fruit fly

Top Publications

  1. Wagner N, Schmitt J, Krohne G. Two novel LEM-domain proteins are splice products of the annotated Drosophila melanogaster gene CG9424 (Bocksbeutel). Eur J Cell Biol. 2004;82:605-16 pubmed
    ..nuclear membrane including the lamina-associated polypeptides 2 (LAP2), emerin, MAN1 and the Drosophila protein otefin. This evolutionary conserved sequence motif can mediate via the interaction with the small protein BAF the binding ..
  2. Ashery Padan R, Ulitzur N, Arbel A, Goldberg M, Weiss A, Maus N, et al. Localization and posttranslational modifications of otefin, a protein required for vesicle attachment to chromatin, during Drosophila melanogaster development. Mol Cell Biol. 1997;17:4114-23 pubmed
    b>Otefin is a peripheral protein of the inner nuclear membrane in Drosophila melanogaster. Here we show that during nuclear assembly in vitro, it is required for the attachment of membrane vesicles to chromatin...
  3. Dialynas G, Speese S, Budnik V, Geyer P, Wallrath L. The role of Drosophila Lamin C in muscle function and gene expression. Development. 2010;137:3067-77 pubmed publisher
    ..These data demonstrate connections among the Drosophila A-type lamin, hormone-induced gene expression and muscle function. ..
  4. McHugh B, Krause S, Yu B, Deans A, Heasman S, McLaughlin P, et al. Invadolysin: a novel, conserved metalloprotease links mitotic structural rearrangements with cell migration. J Cell Biol. 2004;167:673-86 pubmed
    ..Thus, invadolysin identifies a new family of conserved metalloproteases whose activity appears to be essential for the coordination of mitotic progression, but which also plays an unexpected role in cell migration. ..
  5. Rohrbaugh M, Clore A, Davis J, Johnson S, Jones B, Jones K, et al. Identification and characterization of proteins involved in nuclear organization using Drosophila GFP protein trap lines. PLoS ONE. 2013;8:e53091 pubmed publisher
    ..Information gained from the analysis of CG11138 Stwl, dNlp, and Df31 sets the stage for future studies of these proteins. ..
  6. Ulitzur N, Harel A, Goldberg M, Feinstein N, Gruenbaum Y. Nuclear membrane vesicle targeting to chromatin in a Drosophila embryo cell-free system. Mol Biol Cell. 1997;8:1439-48 pubmed
    ..These chromatin-attached vesicles contained lamin Dm and otefin but not gp210. Thus, these results show that in Drosophila there are two populations of nuclear vesicles...
  7. Busayavalasa K, Chen X, Farrants A, Wagner N, Sabri N. The Nup155-mediated organisation of inner nuclear membrane proteins is independent of Nup155 anchoring to the metazoan nuclear pore complex. J Cell Sci. 2012;125:4214-8 pubmed publisher
    ..the ?-solenoid part of Nup155 is essential for the correct localisation of INM proteins lamin-B receptor (LBR) and otefin. Using chromatin extracts from semi-synchronized cells, we also provide evidence that the Nup155 ?-solenoid has a ..
  8. Furukawa K, Ishida K, Tsunoyama T, Toda S, Osoda S, Horigome T, et al. A-type and B-type lamins initiate layer assembly at distinct areas of the nuclear envelope in living cells. Exp Cell Res. 2009;315:1181-9 pubmed publisher
    ..This previously unknown complexity in the assembly of the nuclear lamina could be the basis for intricate nuclear envelope functions. ..
  9. Wagner N, Kagermeier B, Loserth S, Krohne G. The Drosophila melanogaster LEM-domain protein MAN1. Eur J Cell Biol. 2006;85:91-105 pubmed
    ..During embryogenesis and early larval development LEM-domain proteins dMAN1 and otefin show the same expression pattern and are much more abundant in eggs and the first larval instar than in later ..

More Information


  1. Padan R, Nainudel Epszteyn S, Goitein R, Fainsod A, Gruenbaum Y. Isolation and characterization of the Drosophila nuclear envelope otefin cDNA. J Biol Chem. 1990;265:7808-13 pubmed
    ..identified and characterized a 53-kDa inner nuclear membrane-associated protein in Drosophila and termed it otefin. Here we report the isolation and characterization of cDNA and genomic clones of the otefin gene...
  2. Wang H, Mu Y, Chen D. Effective gene silencing in Drosophila ovarian germline by artificial microRNAs. Cell Res. 2011;21:700-3 pubmed publisher
  3. Hita M, Poirie M, Leblanc N, Lemeunier F, Lutcher F, Frey F, et al. Genetic localization of a Drosophila melanogaster resistance gene to a parasitoid wasp and physical mapping of the region. Genome Res. 1999;9:471-81 pubmed
    ..Using single and double digests, cross hybridization of restriction fragments, and location of genetically mapped genes and STSs, a complete, five-enzyme restriction map of this 830-kb region was obtained. ..
  4. Mohr S, Gelbart W. Using the P[wHy] hybrid transposable element to disrupt genes in region 54D-55B in Drosophila melanogaster. Genetics. 2002;162:165-76 pubmed
    ..Thus, the P[wHy] method provides an efficient method for systematically determining the phenotypes of genes in a given region of the fly genome. ..
  5. Ali I, ur Rehman M, Rashid F, Khan S, Iqbal A, Laixin X, et al. Cis-regulatory elements affecting the Nanos gene promoter in the germline stem cells. J Biotechnol. 2010;145:323-9 pubmed publisher
  6. Jokhi V, Ashley J, Nunnari J, Noma A, Ito N, Wakabayashi Ito N, et al. Torsin mediates primary envelopment of large ribonucleoprotein granules at the nuclear envelope. Cell Rep. 2013;3:988-95 pubmed publisher
  7. Milon B, Cheng H, Tselebrovsky M, Lavrov S, Nenasheva V, Mikhaleva E, et al. Role of histone deacetylases in gene regulation at nuclear lamina. PLoS ONE. 2012;7:e49692 pubmed publisher
    ..These findings demonstrate the manifold and central involvement of class I HDACs in regulation of lamina-associated genes, illuminating a mechanism by which these enzymes can orchestrate normal and pathological development...
  8. Berman B, Pfeiffer B, Laverty T, Salzberg S, Rubin G, Eisen M, et al. Computational identification of developmental enhancers: conservation and function of transcription factor binding-site clusters in Drosophila melanogaster and Drosophila pseudoobscura. Genome Biol. 2004;5:R61 pubmed
    ..Measuring conservation of sequence features closely linked to function--such as binding-site clusterin--makes better use of comparative sequence data than commonly used methods that examine only sequence identity. ..
  9. Wagner N, Weber D, Seitz S, Krohne G. The lamin B receptor of Drosophila melanogaster. J Cell Sci. 2004;117:2015-28 pubmed
    ..We conclude that dLBR is not a limiting component of the nuclear architecture in Drosophila cells during the first 2 days of development. ..
  10. Jiang X, Xia L, Chen D, Yang Y, Huang H, Yang L, et al. Otefin, a nuclear membrane protein, determines the fate of germline stem cells in Drosophila via interaction with Smad complexes. Dev Cell. 2008;14:494-506 pubmed publisher
    ..We found that otefin (ote), which encodes a nuclear lamin-binding protein [corrected], is essential for germline stem cell (GSC) ..
  11. Barton L, Pinto B, Wallrath L, Geyer P. The Drosophila nuclear lamina protein otefin is required for germline stem cell survival. Dev Cell. 2013;25:645-54 pubmed publisher
    ..Defects in LEM-D proteins cause tissue-restricted human diseases associated with altered stem cell homeostasis. Otefin (Ote) is a Drosophila LEM-D protein that is intrinsically required for female germline stem cell (GSC) maintenance...
  12. Bachtrog D, Agis M, Imhof M, Schlotterer C. Microsatellite variability differs between dinucleotide repeat motifs-evidence from Drosophila melanogaster. Mol Biol Evol. 2000;17:1277-85 pubmed
    ..Similar differences in mutation rates were obtained by an alternative method which estimates microsatellite mutation rates from their genomic length distribution. ..
  13. Sui L, Yang Y. Distinct effects of nuclear membrane localization on gene transcription silencing in Drosophila S2 cells and germ cells. J Genet Genomics. 2011;38:55-61 pubmed publisher
    ..A previous study reported that the inner nuclear membrane protein, Otefin (Ote), was essential for germline stem cell (GSC) maintenance via interaction with Smad complex...
  14. Goldberg M, Lu H, Stuurman N, Ashery Padan R, Weiss A, Yu J, et al. Interactions among Drosophila nuclear envelope proteins lamin, otefin, and YA. Mol Cell Biol. 1998;18:4315-23 pubmed
    ..Lamin, otefin, and YA are the three Drosophila nuclear envelope proteins known in early embryos...
  15. Habermann K, Mirgorodskaya E, Gobom J, Lehmann V, Müller H, Blümlein K, et al. Functional analysis of centrosomal kinase substrates in Drosophila melanogaster reveals a new function of the nuclear envelope component otefin in cell cycle progression. Mol Cell Biol. 2012;32:3554-69 pubmed publisher
    ..Among those was otefin (Ote), an NE protein that we found to localize to centrosomes...
  16. Barton L, Wilmington S, Martin M, Skopec H, Lovander K, Pinto B, et al. Unique and shared functions of nuclear lamina LEM domain proteins in Drosophila. Genetics. 2014;197:653-65 pubmed publisher
    ..Drosophila melanogaster has three nuclear lamina LEM-D proteins, named Otefin (Ote), Bocksbeutel (Bocks), and dMAN1...
  17. Pirone L, Xolalpa W, Sigurðsson J, Ramirez J, Pérez C, Gonzalez M, et al. A comprehensive platform for the analysis of ubiquitin-like protein modifications using in vivo biotinylation. Sci Rep. 2017;7:40756 pubmed publisher
    ..Ease of use and the flexibility to modify existing vectors will make the bioUbL system a powerful complement to existing strategies for studying this important mode of protein regulation. ..
  18. Lancaster O, Cullen C, Ohkura H. NHK-1 phosphorylates BAF to allow karyosome formation in the Drosophila oocyte nucleus. J Cell Biol. 2007;179:817-24 pubmed
    ..We propose that BAF phosphorylation by NHK-1 disrupts anchorage of chromosomes to the nuclear envelope, allowing karyosome formation in oocytes. These data provide the first mechanistic insight into how the karyosome forms. ..
  19. Pinto B, Wilmington S, Hornick E, Wallrath L, Geyer P. Tissue-specific defects are caused by loss of the Drosophila MAN1 LEM domain protein. Genetics. 2008;180:133-45 pubmed publisher
    ..The tissue-specific, age-enhanced dMAN1 mutant phenotypes are reminiscent of human laminopathies, suggesting that studies in Drosophila will provide insights into lamina dysfunction associated with disease. ..
  20. Kusch T. Brca2-Pds5 complexes mobilize persistent meiotic recombination sites to the nuclear envelope. J Cell Sci. 2015;128:717-27 pubmed publisher
    ..The identification of Brca2-Pds5 complexes as key mediators of this process provides a first mechanistic explanation for the contribution of lamins and cohesins to meiotic recombination. ..
  21. Harel A, Zlotkin E, Nainudel Epszteyn S, Feinstein N, Fisher P, Gruenbaum Y. Persistence of major nuclear envelope antigens in an envelope-like structure during mitosis in Drosophila melanogaster embryos. J Cell Sci. 1989;94 ( Pt 3):463-70 pubmed
    ..Two of these proteins, lamin and otefin, a newly characterized nuclear envelope polypeptide with an apparent Mr of 53,000, are apparently present in an ..
  22. Ji J, Crest J, Schubiger G. Genetic interactions between Cdk1-CyclinB and the Separase complex in Drosophila. Development. 2005;132:1875-84 pubmed
  23. Ashery Padan R, Weiss A, Feinstein N, Gruenbaum Y. Distinct regions specify the targeting of otefin to the nucleoplasmic side of the nuclear envelope. J Biol Chem. 1997;272:2493-9 pubmed
    b>Otefin is a 45-kDa nuclear envelope protein with no apparent homology to other known proteins...
  24. Barton L, Lovander K, Pinto B, Geyer P. Drosophila male and female germline stem cell niches require the nuclear lamina protein Otefin. Dev Biol. 2016;415:75-86 pubmed publisher
    ..Here we present our studies of one of the Drosophila nuclear lamina LEM-D proteins, Otefin (Ote), a homologue of emerin...