okr

Summary

Gene Symbol: okr
Description: okra
Alias: CG3736, DhR54, DmRAD54, DmRad54, Dmel\CG3736, Dmrad54, RAD54, RAD54L, Rad54, Ras54, rad54, CG3736-PA, okr-PA
Species: fruit fly

Top Publications

  1. pmc Drosophila brca2 is required for mitotic and meiotic DNA repair and efficient activation of the meiotic recombination checkpoint
    Martha Klovstad
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS Genet 4:e31. 2008
  2. pmc The Drosophila spn-D gene encodes a RAD51C-like protein that is required exclusively during meiosis
    Uri Abdu
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Genetics 165:197-204. 2003
  3. pmc Lig4 and rad54 are required for repair of DNA double-strand breaks induced by P-element excision in Drosophila
    Ron J Romeijn
    Department of Toxicogenetics, Leiden University Medical Center LUMC, 2333 AL, Leiden, The Netherlands
    Genetics 169:795-806. 2005
  4. pmc The Drosophila melanogaster DNA Ligase IV gene plays a crucial role in the repair of radiation-induced DNA double-strand breaks and acts synergistically with Rad54
    Marcin M Gorski
    Department of Toxicogenetics, Leiden University Medical Center, 2333 AL, Leiden, The Netherlands
    Genetics 165:1929-41. 2003
  5. pmc Mutations in the extra sex combs and Enhancer of Polycomb genes increase homologous recombination in somatic cells of Drosophila melanogaster
    Angela M Holmes
    Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario N6A 5C1, Canada
    Genetics 172:2367-77. 2006
  6. pmc Multiple-pathway analysis of double-strand break repair mutations in Drosophila
    Dena M Johnson-Schlitz
    Department of Genetics, University of Wisconsin, Madison, Wisconsin, United States of America
    PLoS Genet 3:e50. 2007
  7. pmc A large-scale screen for mutagen-sensitive loci in Drosophila
    Anne Laurencon
    Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
    Genetics 167:217-31. 2004
  8. pmc The Drosophila melanogaster RAD54 homolog, DmRAD54, is involved in the repair of radiation damage and recombination
    R Kooistra
    Department of Radiation Genetics and Chemical Mutagenesis, MGC, Leiden University, The Netherlands
    Mol Cell Biol 17:6097-104. 1997
  9. pmc Drosophila PCH2 is required for a pachytene checkpoint that monitors double-strand-break-independent events leading to meiotic crossover formation
    Eric F Joyce
    Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854 8020, USA
    Genetics 181:39-51. 2009
  10. ncbi Activation of a meiotic checkpoint during Drosophila oogenesis regulates the translation of Gurken through Chk2/Mnk
    Uri Abdu
    HHMI, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Curr Biol 12:1645-51. 2002

Scientific Experts

  • A Pastink
  • Cathleen M Lake
  • J Thacker
  • J D Schnorr
  • P G de Buendía
  • Uri Abdu
  • Sarit Cohen
  • Vassilios Alexiadis
  • Kim S McKim
  • Rotem Kadir
  • Ron J Romeijn
  • Irene Chiolo
  • Marcin M Gorski
  • Wan Jin Lu
  • Elizabeth A Manheim
  • Ana Bozas
  • Eric F Joyce
  • Jonathan I Schneiderman
  • Ivy E McDaniel
  • Kamilla Sofie Pedersen
  • Martha Klovstad
  • Hifzur R Siddique
  • Debbie S Wei
  • Dena M Johnson-Schlitz
  • Vitor Barbosa
  • S Mehrotra
  • Angela M Holmes
  • R Kooistra
  • Leon H Mullenders
  • Wouter Ferro
  • Anne Laurencon
  • Burnley R Jaklevic
  • James T Kadonaga
  • Seth D Findley
  • Janet K Jang
  • Ronit Tokarsky
  • Anna Bakhrat
  • Sylvain V Costes
  • Gary H Karpen
  • Aki Minoda
  • Serafin U Colmenares
  • Aris Polyzos
  • Ignasi Roig
  • Joseph Chapo
  • John M Abrams
  • Dana Carroll
  • Kelly J Beumer
  • Jonathan K Trautman
  • Akiko Sakai
  • Kami Ahmad
  • Sara Goldstein
  • Volker Loeschcke
  • Jens Ø Duus
  • Jennifer M Lee
  • Cori K Hanagami
  • Bent O Petersen
  • J Morris
  • Matthew S Berger
  • Anurag Sharma
  • Debapratim K Chowdhuri
  • Alok Dhawan
  • Trudi Schupbach
  • Anders Malmendal
  • Niels Chr Nielsen
  • Jennifer A Armstrong
  • Ramesh C Murthy
  • Torsten Nygaard Kristensen
  • Daya K Saxena
  • Subash C Gupta
  • Carlos Flores
  • Naomi Kimm
  • William R Engels
  • Ruth Lehmann
  • Yikang S Rong
  • J C Eeken
  • J B Zonneveld
  • K S McKim
  • Gregory B Gloor
  • P H Lohman
  • Kelly A Weedmark
  • Jasprina N Noordermeer
  • Martijn A van Schie
  • Sasha A Langley
  • R Scott Hawley
  • Nathan J Harris
  • Charisse M Orme
  • Tin Tin Su
  • Alexandra Lusser
  • Kenneth C Burtis
  • Christina L Boulton

Detail Information

Publications38

  1. pmc Drosophila brca2 is required for mitotic and meiotic DNA repair and efficient activation of the meiotic recombination checkpoint
    Martha Klovstad
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS Genet 4:e31. 2008
    ..In addition, Brca2 co-immunoprecipitates with the checkpoint protein Rad9, suggesting a direct role for Brca2 in the transduction of the meiotic recombination checkpoint signal...
  2. pmc The Drosophila spn-D gene encodes a RAD51C-like protein that is required exclusively during meiosis
    Uri Abdu
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    Genetics 165:197-204. 2003
    ..As there is no apparent ortholog of the meiosis-specific DMC1 gene in the Drosophila genome, and given their meiosis-specific requirement, we suggest that spn-B and spn-D may have a function comparable to DMC1...
  3. pmc Lig4 and rad54 are required for repair of DNA double-strand breaks induced by P-element excision in Drosophila
    Ron J Romeijn
    Department of Toxicogenetics, Leiden University Medical Center LUMC, 2333 AL, Leiden, The Netherlands
    Genetics 169:795-806. 2005
    ..the repair of these breaks, the w(hd) P-element was mobilized in flies carrying mutant alleles of either lig4 or rad54. The survival of both lig4- and rad54-deficient males was reduced to 25% in comparison to the wild type, ..
  4. pmc The Drosophila melanogaster DNA Ligase IV gene plays a crucial role in the repair of radiation-induced DNA double-strand breaks and acts synergistically with Rad54
    Marcin M Gorski
    Department of Toxicogenetics, Leiden University Medical Center, 2333 AL, Leiden, The Netherlands
    Genetics 165:1929-41. 2003
    ..To determine the relative contribution of NHEJ and homologous recombination (HR) in Drosophila, Lig4; Rad54 double-mutant flies were generated...
  5. pmc Mutations in the extra sex combs and Enhancer of Polycomb genes increase homologous recombination in somatic cells of Drosophila melanogaster
    Angela M Holmes
    Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario N6A 5C1, Canada
    Genetics 172:2367-77. 2006
    ..We propose that esc and E(Pc) mutants enhance homologous recombination by modulating the histone acetylation status of histone H4 at the double-strand break...
  6. pmc Multiple-pathway analysis of double-strand break repair mutations in Drosophila
    Dena M Johnson-Schlitz
    Department of Genetics, University of Wisconsin, Madison, Wisconsin, United States of America
    PLoS Genet 3:e50. 2007
    ....
  7. pmc A large-scale screen for mutagen-sensitive loci in Drosophila
    Anne Laurencon
    Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
    Genetics 167:217-31. 2004
    ..At least one allele of each new gene identified in this study is available at the Bloomington Stock Center...
  8. pmc The Drosophila melanogaster RAD54 homolog, DmRAD54, is involved in the repair of radiation damage and recombination
    R Kooistra
    Department of Radiation Genetics and Chemical Mutagenesis, MGC, Leiden University, The Netherlands
    Mol Cell Biol 17:6097-104. 1997
    ..Here the isolation and functional characterization of the RAD54 homolog of the fruit fly Drosophila melanogaster, DmRAD54, are described. The putative Dmrad54 protein displays 46 to 57% identity to its homologs from yeast and mammals...
  9. pmc Drosophila PCH2 is required for a pachytene checkpoint that monitors double-strand-break-independent events leading to meiotic crossover formation
    Eric F Joyce
    Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854 8020, USA
    Genetics 181:39-51. 2009
    ..Interestingly, PCH2-dependent delays in prophase may allow additional crossovers to form...
  10. ncbi Activation of a meiotic checkpoint during Drosophila oogenesis regulates the translation of Gurken through Chk2/Mnk
    Uri Abdu
    HHMI, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Curr Biol 12:1645-51. 2002
    ..However, the components of this checkpoint in flies have not been completely elucidated...
  11. pmc Temporal analysis of meiotic DNA double-strand break formation and repair in Drosophila females
    S Mehrotra
    Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America
    PLoS Genet 2:e200. 2006
    ..From a comparison of the frequency of gamma-His2Av foci and crossovers, it appears that Drosophila females have only a weak mechanism to ensure a crossover in the presence of a low number of DSBs...
  12. ncbi The Synaptonemal complex component C(2)M regulates meiotic crossing over in Drosophila
    Elizabeth A Manheim
    Waksman Institute and Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 8020, USA
    Curr Biol 13:276-85. 2003
    ..Previous studies have suggested that the SC is required for meiotic crossing over in Drosophila. However, only one component of this structure, C(3)G, has been identified in Drosophila...
  13. pmc An essential role for Drosophila hus1 in somatic and meiotic DNA damage responses
    Uri Abdu
    Department of Life Sciences, National Institute for Biotechnology in the Negev, Ben Gurion University, Beer Sheva, 84105, Israel
    J Cell Sci 120:1042-9. 2007
    ..These results demonstrate that hus1 is essential for the activation of the meiotic checkpoint and that hus1 is also required for the organization of the oocyte DNA, a function that might be independent of the meiotic checkpoint...
  14. ncbi Relationship of DNA double-strand breaks to synapsis in Drosophila
    Janet K Jang
    Waksman Institute and Department of Genetics, Rutgers, The State University of New Jersey, 190 Frelinghuysen Rd, Piscataway, New Jersey 08854 8020, USA
    J Cell Sci 116:3069-77. 2003
    ..Furthermore, mutations in Drosophila Rad54 (okr) and Rad51 (spnB) homologs cause delayed and prolonged gamma-HIS2AV staining, suggesting that double-strand ..
  15. pmc Double-strand breaks in heterochromatin move outside of a dynamic HP1a domain to complete recombinational repair
    Irene Chiolo
    Department of Genome Dynamics, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Cell 144:732-44. 2011
    ..We propose that the spatial and temporal control of DSB repair in heterochromatin safeguards genome stability by preventing aberrant exchanges between repeats...
  16. doi DNA damage induced by industrial solid waste leachates in Drosophila melanogaster: a mechanistic approach
    Hifzur R Siddique
    Embryotoxicology Section, Industrial Toxicology Research Centre, Lucknow, Uttar Pradesh, India
    Environ Mol Mutagen 49:206-16. 2008
    ..protein), mus201 (deficient in excision repair protein), mus308 (deficient in postreplication repair protein), and rad54 (deficient in double strand break repair protein) than in the OregonR(+) wild-type strain...
  17. ncbi Genomic integrity and the repair of double-strand DNA breaks
    A Pastink
    Sylvius Laboratory, Department of Radiation Genetics and Chemical Mutagenesis, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
    Mutat Res 480:37-50. 2001
    ..This review summarizes our current knowledge about DSB-repair with emphasis on recent progress in understanding the precise biochemical activities of individual proteins involved...
  18. pmc Meiotic recombination provokes functional activation of the p53 regulatory network
    Wan Jin Lu
    Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Science 328:1278-81. 2010
    ..Our findings establish a physiological role for p53 in meiosis and suggest that tumor-suppressive functions may have been co-opted from primordial activities linked to recombination...
  19. pmc The Drosophila zinc finger protein trade embargo is required for double strand break formation in meiosis
    Cathleen M Lake
    Stowers Institute for Medical Research, Kansas City, Missouri, United States of America
    PLoS Genet 7:e1002005. 2011
    ..We speculate that Trem may act by either directing the binding of Mei-P22 to preferred sites of DSB formation or by altering chromatin structure in a manner that allows Mei-P22 to form foci...
  20. pmc Localization of the Drosophila Rad9 protein to the nuclear membrane is regulated by the C-terminal region and is affected in the meiotic checkpoint
    Rotem Kadir
    Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
    PLoS ONE 7:e38010. 2012
    ..Thus, by studying the localization pattern of DmRad9, our study reveals that the DmRad9A C-terminal region targets the protein to the nuclear membrane, where it might play a role in response to the activation of the meiotic checkpoint...
  21. ncbi A conserved N-terminal motif in Rad54 is important for chromatin remodeling and homologous strand pairing
    Vassilios Alexiadis
    Section of Molecular Biology, University of California, San Diego, La Jolla, California 92093 0347, USA
    J Biol Chem 279:27824-9. 2004
    The Swi2/Snf2-related protein Rad54 is a chromatin remodeling enzyme that is important for homologous strand pairing catalyzed by the eukaryotic recombinase Rad51...
  22. ncbi Search for DNA repair pathways in Drosophila melanogaster
    P G de Buendía
    Instituto de Genetica, Universidad de Los Andes, Bogota, Colombia
    Mutat Res 407:67-84. 1998
    ..A possible explanation could be a weaker control of post-replication repair mechanisms in Drosophila than in yeast. It is expected that this research could help for a better understanding of repair mechanisms in complex organisms...
  23. ncbi Drosophila oogenesis: versatile spn doctors
    J Morris
    Developmental Genetics Program, Skirball Institute, Howard Hughes Medical Institute, Department of Cell Biology, NYU School of Medicine, 540 First Avenue, New York, 10016, USA
    Curr Biol 9:R55-8. 1999
    ..Genes of the spindle class, which encode double-strand break repair enzymes and RNA helicases, affect oocyte polarity and the decision whether to differentiate as an oocyte or a nurse cell...
  24. ncbi A surfeit of RAD51-like genes?
    J Thacker
    Medical Research Council, Radiation and Genome Stability Unit, Harwell, Oxfordshire, UK OX11 0RD
    Trends Genet 15:166-8. 1999
    ..The recent cloning and functional analysis of eukaryotic recombination genes suggests that at least part of the intricate mechanism involved is conserved from bacteria to humans, but is also yielding some surprises...
  25. pmc The XNP remodeler targets dynamic chromatin in Drosophila
    Jonathan I Schneiderman
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 106:14472-7. 2009
    ..Thus, the XNP focus appears to be a functional genetic element that can contribute to gene silencing throughout the nucleus...
  26. pmc Genetic analysis of zinc-finger nuclease-induced gene targeting in Drosophila
    Ana Bozas
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112 5650, USA
    Genetics 182:641-51. 2009
    ..The frequency of HR dropped significantly in flies homozygous for mutations in spnA (Rad51) or okr (Rad54), two components of the invasion-mediated synthesis-dependent strand annealing (SDSA) pathway...
  27. pmc The Drosophila melanogaster DmRAD54 gene plays a crucial role in double-strand break repair after P-element excision and acts synergistically with Ku70 in the repair of X-ray damage
    R Kooistra
    Department of Radiation Genetics and Chemical Mutagenesis, MGC, Leiden University Medical Center, Leiden, The Netherlands
    Mol Cell Biol 19:6269-75. 1999
    ..A Drosophila melanogaster strain deficient in the RAD54 homolog DmRAD54 is characterized by increased X-ray and methyl methanesulfonate (MMS) sensitivity...
  28. pmc Metabolomic signatures of inbreeding at benign and stressful temperatures in Drosophila melanogaster
    Kamilla Sofie Pedersen
    Department of Biological Sciences, University of Aarhus, DK 8000 Aarhus C, Denmark
    Genetics 180:1233-43. 2008
    ....
  29. ncbi Repair and consequences of double-strand breaks in DNA
    A Pastink
    Department of Radiation Genetics and Chemical Mutagenesis, MGC, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL, Leiden, Netherlands
    Mutat Res 428:141-56. 1999
  30. pmc Ras1 interacts with multiple new signaling and cytoskeletal loci in Drosophila eggshell patterning and morphogenesis
    J D Schnorr
    Department of Biology, Whitman College, Walla Walla, Washington 99362, USA
    Genetics 159:609-22. 2001
    ..Mutations in Star and l(2)43Bb had no significant effect upon the lambdatop eggshell defect whereas smt3 and dock alleles significantly suppressed the lambdatop phenotype...
  31. pmc Investigations of CHD1 function in transcription and development of Drosophila melanogaster
    Ivy E McDaniel
    Joint Science Department, W M Keck Science Center, Scripps, Claremont McKenna, and Pitzer Colleges, Claremont, California 91711, USA
    Genetics 178:583-7. 2008
    ..These results clarify the roles of chromatin remodelers in transcription and provide novel insights into CHD1 function...
  32. pmc A genetic screen for DNA double-strand break repair mutations in Drosophila
    Debbie S Wei
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genetics 177:63-77. 2007
    ..We show that Drosophila Rad51 and Rad54 are both required for homologous repair by gene conversion, but are dispensable for single-strand annealing repair...
  33. pmc A maternal screen for genes regulating Drosophila oocyte polarity uncovers new steps in meiotic progression
    Vitor Barbosa
    Howard Hughes Medical Institute and the Kimmel Center for Biology and Medicine, Skirball Institute, 540 First Avenue, New York, NY 10016, USA
    Genetics 176:1967-77. 2007
    ....
  34. pmc Strand pairing by Rad54 and Rad51 is enhanced by chromatin
    Vassilios Alexiadis
    Section of Molecular Biology, University of California, San Diego, La Jolla, California 92093 0347, USA
    Genes Dev 16:2767-71. 2002
    We investigated the role of chromatin in the catalysis of homologous strand pairing by Rad54 and Rad51. Rad54 is related to the ATPase subunits of chromatin-remodeling factors, whereas Rad51 is related to bacterial RecA...
  35. ncbi Maelstrom, a Drosophila spindle-class gene, encodes a protein that colocalizes with Vasa and RDE1/AGO1 homolog, Aubergine, in nuage
    Seth D Findley
    Department of Biochemistry, Box 357350, University of Washington, Seattle, WA 98195 7350, USA
    Development 130:859-71. 2003
    ..Furthermore, maelstrom mutant ovaries show mislocalization of two proteins involved in the microRNA and/or RNAi pathways, Dicer and Argonaute2, suggesting a potential connection between nuage and the microRNA-pathway...
  36. pmc Extrachromosomal circular DNA of tandemly repeated genomic sequences in Drosophila
    Sarit Cohen
    Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv 69978, Israel
    Genome Res 13:1133-45. 2003
    ..Mutations in the genes okra, mus309, and mei41 did not affect eccDNA under normal conditions or following mutagen treatment, implying that eccDNA formation is different from known pathways of DNA repair...
  37. ncbi Relative contribution of DNA repair, cell cycle checkpoints, and cell death to survival after DNA damage in Drosophila larvae
    Burnley R Jaklevic
    MCD Biology, University of Colorado, Boulder, CO 80309, USA
    Curr Biol 14:23-32. 2004
    ..Cell cycle regulation and DNA repair appear essential for unicellular systems to survive DNA damage. The relative importance of these responses and apoptosis for surviving DNA damage in multicellular organisms remains unclear...
  38. ncbi Novel genetic screen for genes involved in posterior body patterning in Drosophila
    J E Wilson
    Department of Biological Sciences, Stanford University, California 94305 5020, USA
    Dev Genet 19:199-209. 1996
    ..These defects are distinct from previously described perturbations in oskar activity and provide new insights into the regulation of oskar...