Genomes and Genes
Gene Symbol: nSyb
Description: neuronal Synaptobrevin
Alias: CG17248, Dmel\CG17248, Dn-syb, N-SYB, N-Syb, N-syb, NSYB, Nsyb, SYB, Syb, Syb62A, n-Syb, n-syb, nSYB, nsyb, syb, neuronal synaptobrevin, CG17248-PA, CG17248-PE, CG17248-PF, CG17248-PG, CG17248-PH, CG17248-PI, CG17248-PJ, CG17248-PK, N-synaptobrevin, Neuronal-synaptobrevin, Synaptobrevin-62A, VAMP3, nSyb-PA, nSyb-PE, nSyb-PF, nSyb-PG, nSyb-PH, nSyb-PI, nSyb-PJ, nSyb-PK, neural-synaptobrevin, neuronal SYNAPTOBREVIN, neuronal synaptogrevin, synaptobrevin
Species: fruit fly
- Loveall B, Deitcher D. The essential role of bursicon during Drosophila development. BMC Dev Biol. 2010;10:92 pubmed publisher..Importantly, we provide new evidence that bursicon release can precede the initiation of larval ecdysis, and that bursicon tans the puparium. Our results firmly establish bursicon signaling as essential to insect growth and development. ..
- Zhang Y, Rodesch C, Broadie K. Living synaptic vesicle marker: synaptotagmin-GFP. Genesis. 2002;34:142-5 pubmed
- Poskanzer K, Fetter R, Davis G. Discrete residues in the c(2)b domain of synaptotagmin I independently specify endocytic rate and synaptic vesicle size. Neuron. 2006;50:49-62 pubmed
- Littleton J, Chapman E, Kreber R, Garment M, Carlson S, Ganetzky B. Temperature-sensitive paralytic mutations demonstrate that synaptic exocytosis requires SNARE complex assembly and disassembly. Neuron. 1998;21:401-13 pubmedThe neuronal SNARE complex is formed via the interaction of synaptobrevin with syntaxin and SNAP-25. Purified SNARE proteins assemble spontaneously, while disassembly requires the ATPase NSF...
- Hamasaka Y, Nassel D. Mapping of serotonin, dopamine, and histamine in relation to different clock neurons in the brain of Drosophila. J Comp Neurol. 2006;494:314-30 pubmed..Thus, serotonergic neurons form functional inputs on the s-LN(v)s in the larval brain and possibly also in adults. ..
- Jhaveri D, Sen A, Rodrigues V. Mechanisms underlying olfactory neuronal connectivity in Drosophila-the atonal lineage organizes the periphery while sensory neurons and glia pattern the olfactory lobe. Dev Biol. 2000;226:73-87 pubmed..The influence of sensory neurons on the development of the olfactory lobe could serve to match and lock peripheral and central properties important for the generation of olfactory behavior. ..
- Yu J, Kanai M, Demir E, Jefferis G, Dickson B. Cellular organization of the neural circuit that drives Drosophila courtship behavior. Curr Biol. 2010;20:1602-14 pubmed publisher..Sex-specific behavior may instead arise through dimorphic circuits in the brain and nerve cord that differentially couple sensory input to motor output. ..
- Petersen L, Stowers R. A Gateway MultiSite recombination cloning toolkit. PLoS ONE. 2011;6:e24531 pubmed publisher..An inventory of entry clones and destination vectors for Gateway MultiSite cloning has also been established (www.gatewaymultisite.org). ..
- Stowers R, Isacoff E. Drosophila huntingtin-interacting protein 14 is a presynaptic protein required for photoreceptor synaptic transmission and expression of the palmitoylated proteins synaptosome-associated protein 25 and cysteine string protein. J Neurosci. 2007;27:12874-83 pubmed..Based on localization of the palmitoyl transferase HIP14 within the presynaptic nerve terminal, we propose palmitoylation as a possible mechanism that may be operating to rapidly regulate synaptic efficacy. ..
- Hiesinger P, Reiter C, Schau H, Fischbach K. Neuropil pattern formation and regulation of cell adhesion molecules in Drosophila optic lobe development depend on synaptobrevin. J Neurosci. 1999;19:7548-56 pubmed..machinery in Drosophila visual system development, we studied the effects of a loss of function of neuronal synaptobrevin, a protein required for synaptic vesicle release...
- DiAntonio A, Burgess R, Chin A, Deitcher D, Scheller R, Schwarz T. Identification and characterization of Drosophila genes for synaptic vesicle proteins. J Neurosci. 1993;13:4924-35 pubmed..One previously reported candidate (syb), a Drosophila homolog of the vamp or synaptobrevin proteins, has been shown to be expressed at very low levels in neurons and is most abundant in the gut...
- Masuda Nakagawa L, Awasaki T, Ito K, O Kane C. Targeting expression to projection neurons that innervate specific mushroom body calyx and antennal lobe glomeruli in larval Drosophila. Gene Expr Patterns. 2010;10:328-37 pubmed publisher..It thus confirms and extends the previous map of AL-calyx connectivity that was based only on randomly labeled single PNs, and provides tools for targeted manipulation of specific PNs for developmental and functional studies. ..
- Bao H, Daniels R, MacLeod G, Charlton M, Atwood H, Zhang B. AP180 maintains the distribution of synaptic and vesicle proteins in the nerve terminal and indirectly regulates the efficacy of Ca2+-triggered exocytosis. J Neurophysiol. 2005;94:1888-903 pubmed..vesicle proteins critical for calcium-mediated exocytosis, synaptotagmin I, cysteine-string protein, and neuronal synaptobrevin, are all mislocalized to the extrasynaptic axonal regions along with Dap160, an active zone marker (nc82), ..
- Helfrich Forster C, Shafer O, Wülbeck C, Grieshaber E, Rieger D, Taghert P. Development and morphology of the clock-gene-expressing lateral neurons of Drosophila melanogaster. J Comp Neurol. 2007;500:47-70 pubmed..Both the LN(d) and the l-LN(v) differentiate during midmetamorphosis. They do so in close proximity to one another and the fifth PDF-negative s-LN(v), suggesting that these cell groups may derive from common precursors. ..
- Sweeney S, Broadie K, Keane J, Niemann H, O Kane C. Targeted expression of tetanus toxin light chain in Drosophila specifically eliminates synaptic transmission and causes behavioral defects. Neuron. 1995;14:341-51 pubmedTetanus toxin cleaves the synaptic vesicle protein synaptobrevin, and the ensuing loss of neurotransmitter exocytosis has implicated synaptobrevin in this process...
- Ashraf S, McLoon A, Sclarsic S, Kunes S. Synaptic protein synthesis associated with memory is regulated by the RISC pathway in Drosophila. Cell. 2006;124:191-205 pubmed..Therefore, we propose that degradative control of the RISC pathway underlies the pattern of synaptic protein synthesis associated with a stable memory. ..
- Zheng X, Zugates C, Lu Z, Shi L, Bai J, Lee T. Baboon/dSmad2 TGF-beta signaling is required during late larval stage for development of adult-specific neurons. EMBO J. 2006;25:615-27 pubmed..Our results suggest that Babo/dSmad2 signaling prior to metamorphosis may be widely required to prepare neurons for the dynamic environment present during metamorphosis. ..
- Kim Y, Lee H, Seong C, Han K. Expression of a D1 dopamine receptor dDA1/DmDOP1 in the central nervous system of Drosophila melanogaster. Gene Expr Patterns. 2003;3:237-45 pubmed..The dDA1 expression in these areas was only detected in adult, but not in third instar larval CNS. ..
- Daniels R, Gelfand M, Collins C, DiAntonio A. Visualizing glutamatergic cell bodies and synapses in Drosophila larval and adult CNS. J Comp Neurol. 2008;508:131-52 pubmed publisher..This description of the glutamatergic system in Drosophila highlights the prevalence of glutamatergic neurons in the CNS and presents tools for future study and manipulation of glutamatergic transmission. ..
- Lohr R, Godenschwege T, Buchner E, Prokop A. Compartmentalization of central neurons in Drosophila: a new strategy of mosaic analysis reveals localization of presynaptic sites to specific segments of neurites. J Neurosci. 2002;22:10357-67 pubmed..This suggests that synaptogenic mechanisms in the CNS might differ from those at neuromuscular junctions. ..
- Tolar L, Pallanck L. NSF function in neurotransmitter release involves rearrangement of the SNARE complex downstream of synaptic vesicle docking. J Neurosci. 1998;18:10250-6 pubmed..Here we report that an SDS-resistant neural SNARE complex, composed of the SNARE polypeptides syntaxin, n-synaptobrevin, and SNAP-25, accumulates in comt mutants at restrictive temperature...
- Rieckhof G, Yoshihara M, Guan Z, Littleton J. Presynaptic N-type calcium channels regulate synaptic growth. J Biol Chem. 2003;278:41099-108 pubmed..Hypomorphic mutations in syntaxin-1A or n-synaptobrevin, which also disrupt neurotransmitter release, did not affect synapse proliferation at the neuromuscular ..
- Larsson M, Domingos A, Jones W, Chiappe M, Amrein H, Vosshall L. Or83b encodes a broadly expressed odorant receptor essential for Drosophila olfaction. Neuron. 2004;43:703-14 pubmed..Consistent with this cellular defect, the Or83b mutation disrupts behavioral and electrophysiological responses to many odorants. Or83b therefore encodes an atypical odorant receptor that plays an essential general role in olfaction. ..
- Daniels R, Collins C, Gelfand M, Dant J, Brooks E, Krantz D, et al. Increased expression of the Drosophila vesicular glutamate transporter leads to excess glutamate release and a compensatory decrease in quantal content. J Neurosci. 2004;24:10466-74 pubmed..These results demonstrate that (1) expression of DVGLUT determines the size and glutamate content of synaptic vesicles and (2) homeostatic mechanisms exist to attenuate the excitatory effects of excess glutamate release. ..
- Bhattacharya S, Stewart B, Niemeyer B, Burgess R, McCabe B, Lin P, et al. Members of the synaptobrevin/vesicle-associated membrane protein (VAMP) family in Drosophila are functionally interchangeable in vivo for neurotransmitter release and cell viability. Proc Natl Acad Sci U S A. 2002;99:13867-72 pubmed..Drosophila has two characterized members of this gene family: synaptobrevin (syb) and neuronal synaptobrevin (n-syb)...
- Hiesinger P, Scholz M, Meinertzhagen I, Fischbach K, Obermayer K. Visualization of synaptic markers in the optic neuropils of Drosophila using a new constrained deconvolution method. J Comp Neurol. 2001;429:277-88 pubmed..The resulting deconvolution method was used to analyze colocalization between the synaptic vesicle marker neuronal synaptobrevin and synaptic and putative synaptic markers in photoreceptor terminals...
- von Philipsborn A, Liu T, Yu J, Masser C, Bidaye S, Dickson B. Neuronal control of Drosophila courtship song. Neuron. 2011;69:509-22 pubmed publisher..These neurons are potentially connected in a functional circuit, with the descending pIP10 neuron linking the brain and thoracic song centers. Sexual dimorphisms in each of these neurons may explain why only males sing. ..
- Sykes P, Condron B. Development and sensitivity to serotonin of Drosophila serotonergic varicosities in the central nervous system. Dev Biol. 2005;286:207-16 pubmed..The effects of serotonin appear to be selective for serotonergic varicosities, as dopaminergic and corazonergic varicosities remain qualitatively intact following serotonin application. ..
- Thum A, Leisibach B, Gendre N, Selcho M, Stocker R. Diversity, variability, and suboesophageal connectivity of antennal lobe neurons in D. melanogaster larvae. J Comp Neurol. 2011;519:3415-32 pubmed publisher..Together with reports from adult Drosophila, these data suggest that wiring variability may be another principle of insect brain organization, in parallel with stereotypy. ..
- Ramaekers A, Magnenat E, Marin E, Gendre N, Jefferis G, Luo L, et al. Glomerular maps without cellular redundancy at successive levels of the Drosophila larval olfactory circuit. Curr Biol. 2005;15:982-92 pubmed..Hence, we propose the Drosophila larva as an "elementary" olfactory model system. ..
- Hiesinger P, Fayyazuddin A, Mehta S, Rosenmund T, Schulze K, Zhai R, et al. The v-ATPase V0 subunit a1 is required for a late step in synaptic vesicle exocytosis in Drosophila. Cell. 2005;121:607-620 pubmed publisher..Our data suggest that Vha100-1 functions downstream of SNAREs in synaptic vesicle fusion. ..
- Hamasaka Y, Wegener C, Nassel D. GABA modulates Drosophila circadian clock neurons via GABAB receptors and decreases in calcium. J Neurobiol. 2005;65:225-40 pubmed..Our results suggest that the s-LN(v)s receive slow inhibitory GABAergic inputs that decrease intracellular calcium of these clock neurons and block their calcium cycling. This response is mediated by postsynaptic GABA(B) receptors. ..
- Gao Q, Yuan B, Chess A. Convergent projections of Drosophila olfactory neurons to specific glomeruli in the antennal lobe. Nat Neurosci. 2000;3:780-5 pubmed..This convergence allows for the formation of an odotopic map in the antennal lobe whose organization could provide a basis for olfactory discrimination in Drosophila. ..
- Deitcher D, Ueda A, Stewart B, Burgess R, Kidokoro Y, Schwarz T. Distinct requirements for evoked and spontaneous release of neurotransmitter are revealed by mutations in the Drosophila gene neuronal-synaptobrevin. J Neurosci. 1998;18:2028-39 pubmed..To investigate the mechanism of transmitter release, we have examined the role of synaptobrevin/VAMP, a protein involved in vesicular docking and/or fusion...
- Yoshihara M, Ueda A, Zhang D, Deitcher D, Schwarz T, Kidokoro Y. Selective effects of neuronal-synaptobrevin mutations on transmitter release evoked by sustained versus transient Ca2+ increases and by cAMP. J Neurosci. 1999;19:2432-41 pubmedb>Synaptobrevin is a key constituent of the synaptic vesicle membrane...
- Raghu S, Joesch M, Borst A, Reiff D. Synaptic organization of lobula plate tangential cells in Drosophila: gamma-aminobutyric acid receptors and chemical release sites. J Comp Neurol. 2007;502:598-610 pubmed..At their axon terminals in the protocerebrum, both cell types express synaptobrevin, suggesting the presence of presynaptic specializations there...
- Ng M, Roorda R, Lima S, Zemelman B, Morcillo P, Miesenbock G. Transmission of olfactory information between three populations of neurons in the antennal lobe of the fly. Neuron. 2002;36:463-74 pubmed..These circuits may serve as dynamic templates that extract higher order features from afferent activity patterns. ..
- Matsuo E, Seki H, Asai T, Morimoto T, Miyakawa H, Ito K, et al. Organization of projection neurons and local neurons of the primary auditory center in the fruit fly Drosophila melanogaster. J Comp Neurol. 2016;524:1099-164 pubmed publisher..Based on these findings, we established the first comprehensive map of secondary auditory interneurons, which indicates the downstream information flow to parallel ascending pathways, multimodal pathways, and descending pathways. ..
- Katsuki T, Ailani D, Hiramoto M, Hiromi Y. Intra-axonal patterning: intrinsic compartmentalization of the axonal membrane in Drosophila neurons. Neuron. 2009;64:188-99 pubmed publisher..We propose that this intra-axonal compartmentalization is an intrinsic property of Drosophila neurons that provides a basis for the structural and functional development of the nervous system. ..
- Xue M, Zhang B. Do SNARE proteins confer specificity for vesicle fusion?. Proc Natl Acad Sci U S A. 2002;99:13359-61 pubmed
- Ang L, Kim J, Stepensky V, Hing H. Dock and Pak regulate olfactory axon pathfinding in Drosophila. Development. 2003;130:1307-16 pubmed..Our findings therefore identify a central signaling module that is used by ONs to project to their cognate glomeruli. ..
- Ohyama T, Verstreken P, Ly C, Rosenmund T, Rajan A, Tien A, et al. Huntingtin-interacting protein 14, a palmitoyl transferase required for exocytosis and targeting of CSP to synaptic vesicles. J Cell Biol. 2007;179:1481-96 pubmed..Our data indicate that HIP14 controls neurotransmitter release by regulating the trafficking of CSP to synapses. ..
- Miller K, DeProto J, Kaufmann N, Patel B, Duckworth A, Van Vactor D. Direct observation demonstrates that Liprin-alpha is required for trafficking of synaptic vesicles. Curr Biol. 2005;15:684-9 pubmed..and ultrastructural levels, we discover aberrant accumulations of synaptic vesicle markers (Synaptotagmin and Synaptobrevin-GFP) and clear-core vesicles along Drosophila Liprin-alpha mutant axons...
- Clandinin T, Lee C, Herman T, Lee R, Yang A, Ovasapyan S, et al. Drosophila LAR regulates R1-R6 and R7 target specificity in the visual system. Neuron. 2001;32:237-48 pubmed..Using single cell mosaics, we demonstrate that LAR controls targeting of R1-R6 and R7 in a cell-autonomous fashion. The phenotypes of LAR mutant R cells are strikingly similar to those seen in N-cadherin mutants. ..
- Ferguson K, Long H, Cameron S, Chang W, Rao Y. The conserved Ig superfamily member Turtle mediates axonal tiling in Drosophila. J Neurosci. 2009;29:14151-9 pubmed publisher..We propose that extrinsic terminal-terminal recognition mediated by Tutl, acts in concert with intrinsic Activin-dependent control of terminal growth, to restrict the connection made by each R7 axon to a single column. ..
- Richardt A, Rybak J, Störtkuhl K, Meinertzhagen I, Hovemann B. Ebony protein in the Drosophila nervous system: optic neuropile expression in glial cells. J Comp Neurol. 2002;452:93-102 pubmed..Possible ways in which the biochemical activity of Ebony might function with respect to histamine release are considered. ..
- Pauls D, Selcho M, Gendre N, Stocker R, Thum A. Drosophila larvae establish appetitive olfactory memories via mushroom body neurons of embryonic origin. J Neurosci. 2010;30:10655-66 pubmed publisher..Based on this approach, we define 10 subdomains of the larval mushroom body that may be implicated in mediating specific interactions between the olfactory pathway, modulatory neurons, and neuronal output. ..
- Niemeyer B, Schwarz T. SNAP-24, a Drosophila SNAP-25 homologue on granule membranes, is a putative mediator of secretion and granule-granule fusion in salivary glands. J Cell Sci. 2000;113 ( Pt 22):4055-64 pubmed
- Dason J, Smith A, Marin L, Charlton M. Vesicular sterols are essential for synaptic vesicle cycling. J Neurosci. 2010;30:15856-65 pubmed publisher..Our results demonstrate the importance of a high vesicular sterol concentration for endocytosis and suggest that vesicular and membrane sterol pools do not readily intermingle during vesicle recycling. ..
- Rister J, Heisenberg M. Distinct functions of neuronal synaptobrevin in developing and mature fly photoreceptors. J Neurobiol. 2006;66:1271-84 pubmedb>Neuronal synaptobrevin (n-Syb, alias VAMP2), a synaptic vesicle membrane protein with a central role in neurotransmission, is specifically cleaved by the light chain of tetanus neurotoxin (TNT) that is known to reliably block ..
- DeMill C, Qiu X, Kisiel M, Bolotta A, Stewart B. Investigation of the juxtamembrane region of neuronal-Synaptobrevin in synaptic transmission at the Drosophila neuromuscular junction. J Neurophysiol. 2014;112:1356-66 pubmed publisherIn this study, the juxtamembrane region of the Drosophila SNARE protein neuronal-Synaptobrevin (n-Syb) was tested for its role in synaptic transmission. A transgenic approach was used to express n-Syb mutant genes...
- Otsuna H, Shinomiya K, Ito K. Parallel neural pathways in higher visual centers of the Drosophila brain that mediate wavelength-specific behavior. Front Neural Circuits. 2014;8:8 pubmed publisher..The present study provides novel insights about how visual information is separated and processed in parallel to achieve robust control of an innate behavior. ..
- Martín Peña A, Acebes A, Rodriguez J, Sorribes A, de Polavieja G, Fernandez Funez P, et al. Age-independent synaptogenesis by phosphoinositide 3 kinase. J Neurosci. 2006;26:10199-208 pubmed..Thus, we propose that PI3K stimulation can be applied to prevent or delay synapse loss in normal aging and in neurological disorders. ..
- Küppers B, Sanchez Soriano N, Letzkus J, Technau G, Prokop A. In developing Drosophila neurones the production of gamma-amino butyric acid is tightly regulated downstream of glutamate decarboxylase translation and can be influenced by calcium. J Neurochem. 2003;84:939-51 pubmed
- Vanlandingham P, Barmchi M, ROYER S, Green R, Bao H, REIST N, et al. AP180 couples protein retrieval to clathrin-mediated endocytosis of synaptic vesicles. Traffic. 2014;15:433-50 pubmed publisher..Finally, AP180 co-immunoprecipitates with SV proteins, including the vesicular glutamate transporter and neuronal synaptobrevin. These results reveal a new mode by which AP180 couples protein retrieval to CME of SVs...
- Rohrbough J, Grotewiel M, Davis R, Broadie K. Integrin-mediated regulation of synaptic morphology, transmission, and plasticity. J Neurosci. 2000;20:6868-78 pubmed..Our results provide direct evidence that Volado regulates functional synaptic plasticity processes and support recent findings implicating integrins in rapid changes in synaptic efficacy and in memory formation. ..
- Soustelle L, Giangrande A. Novel gcm-dependent lineages in the postembryonic nervous system of Drosophila melanogaster. Dev Dyn. 2007;236:2101-8 pubmed..It is also expressed in a thoracic glial lineage and in neurons of the ventral nerve cord (VNC). Finally, while gcm is required for gliogenesis in medulla and VNC, it does not seem to be required for the generation of VNC neurons. ..
- Silbering A, Rytz R, Grosjean Y, Abuin L, Ramdya P, Jefferis G, et al. Complementary function and integrated wiring of the evolutionarily distinct Drosophila olfactory subsystems. J Neurosci. 2011;31:13357-75 pubmed publisher..We integrate knowledge on the different phylogenetic and developmental properties of these receptors and circuits to propose models for the functional contributions and evolution of these distinct olfactory subsystems...
- Toonen R, Verhage M. Vesicle trafficking: pleasure and pain from SM genes. Trends Cell Biol. 2003;13:177-86 pubmed..This review examines whether a universal principle for the molecular function of SM genes exists and whether the divergence in SM gene function can be related to the unique characteristics of different transport routes. ..
- Gao X, Clandinin T, Luo L. Extremely sparse olfactory inputs are sufficient to mediate innate aversion in Drosophila. PLoS ONE. 2015;10:e0125986 pubmed publisher..Our data demonstrate the robustness of olfactory aversion, and suggest that its circuit mechanism is qualitatively different from attraction. ..
- Yu W, Kawasaki F, Ordway R. Activity-dependent interactions of NSF and SNAP at living synapses. Mol Cell Neurosci. 2011;47:19-27 pubmed publisher..These observations extend current models describing the spatial organization of NSF, SNAP and SNARE proteins in synaptic vesicle trafficking. ..
- Devaud J, Acebes A, Ramaswami M, Ferrús A. Structural and functional changes in the olfactory pathway of adult Drosophila take place at a critical age. J Neurobiol. 2003;56:13-23 pubmed..Taken together, these data demonstrate an imprinting-like phenomenon in the olfactory pathway of young Drosophila adults, and illustrate its glomerulus-specific dynamics. ..
- Poskanzer K, Marek K, Sweeney S, Davis G. Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo. Nature. 2003;426:559-63 pubmed..By inactivating Syt I only during endocytosis, we demonstrate that Syt I is necessary for the endocytosis of synaptic vesicles that have undergone exocytosis using a functional Syt I protein. ..
- Haberman A, Williamson W, Epstein D, Wang D, Rina S, Meinertzhagen I, et al. The synaptic vesicle SNARE neuronal Synaptobrevin promotes endolysosomal degradation and prevents neurodegeneration. J Cell Biol. 2012;196:261-76 pubmed publisher..The neuron-specific synaptic v-SNARE n-syb (neuronal Synaptobrevin) plays a key role during synaptic vesicle exocytosis...