Not1

Summary

Gene Symbol: Not1
Description: Not1
Alias: CG1874, CG1884, CG34407, Dmel\CG34407, Dmel_CG1874, Dmel_CG1884, NOT, NOT1, anon-EST:Liang-1.29, anon-WO0118547.221, clone 1.29, not1, Not1, CG34407-PC, CG34407-PD, CG34407-PE, CG34407-PF, CG34407-PG, CG34407-PH, Not1-PC, Not1-PD, Not1-PE, Not1-PF, Not1-PG, Not1-PH
Species: fruit fly

Top Publications

  1. Fukaya T, Tomari Y. MicroRNAs mediate gene silencing via multiple different pathways in drosophila. Mol Cell. 2012;48:825-36 pubmed publisher
    ..Differential contribution from these multiple pathways may explain previous, apparently contradictory observations of how microRNAs inhibit protein synthesis. ..
  2. Semotok J, Cooperstock R, Pinder B, Vari H, Lipshitz H, Smibert C. Smaug recruits the CCR4/POP2/NOT deadenylase complex to trigger maternal transcript localization in the early Drosophila embryo. Curr Biol. 2005;15:284-94 pubmed
    ..In smaug mutants, degradation of Hsp83 transcripts is not triggered, and, thus, localization does not occur...
  3. Temme C, Zhang L, Kremmer E, Ihling C, Chartier A, Sinz A, et al. Subunits of the Drosophila CCR4-NOT complex and their roles in mRNA deadenylation. RNA. 2010;16:1356-70 pubmed publisher
    ..of this complex in Drosophila melanogaster by immunoprecipitation with a monoclonal antibody directed against NOT1. The CCR4, CAF1 (=POP2), NOT1, NOT2, NOT3, and CAF40 subunits were associated in a stable complex, but NOT4 was not...
  4. Braun J, Huntzinger E, Fauser M, Izaurralde E. GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets. Mol Cell. 2011;44:120-33 pubmed publisher
    ..proteins recruit the PAN2-PAN3 and CCR4-CAF1-NOT deadenylase complexes through direct interactions with PAN3 and NOT1, respectively. These interactions are critical for silencing and are conserved in D. melanogaster...
  5. Huntzinger E, Kuzuoğlu Öztürk D, Braun J, Eulalio A, Wohlbold L, Izaurralde E. The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets. Nucleic Acids Res. 2013;41:978-94 pubmed publisher
    ..In turn, GW182 proteins interact with poly(A)-binding protein (PABP) and the PAN2-PAN3 and CCR4-NOT deadenylase complexes. These interactions are required for the deadenylation and decay of miRNA targets...
  6. Eulalio A, Huntzinger E, Nishihara T, Rehwinkel J, Fauser M, Izaurralde E. Deadenylation is a widespread effect of miRNA regulation. RNA. 2009;15:21-32 pubmed publisher
    ..In animal cells, degradation of partially complementary miRNA targets occurs via deadenylation by the CAF1-CCR4-NOT1 deadenylase complex, followed by decapping and subsequent exonucleolytic digestion...
  7. Temme C, Zaessinger S, Meyer S, Simonelig M, Wahle E. A complex containing the CCR4 and CAF1 proteins is involved in mRNA deadenylation in Drosophila. EMBO J. 2004;23:2862-71 pubmed
    The CCR4-NOT complex is the major enzyme catalyzing mRNA deadenylation in Saccharomyces cerevisiae. We have identified homologs for almost all subunits of this complex in the Drosophila genome...
  8. Zekri L, Huntzinger E, Heimstädt S, Izaurralde E. The silencing domain of GW182 interacts with PABPC1 to promote translational repression and degradation of microRNA targets and is required for target release. Mol Cell Biol. 2009;29:6220-31 pubmed publisher
    ..miRNA targets at a step of silencing downstream of deadenylation, indicating that GW182 is required to initiate but not to maintain silencing...
  9. Collart M, Panasenko O. The Ccr4--not complex. Gene. 2012;492:42-53 pubmed publisher
    The Ccr4-Not complex is a unique, essential and conserved multi-subunit complex that acts at the level of many different cellular functions to regulate gene expression...

More Information

Publications22

  1. Petit A, Wohlbold L, Bawankar P, Huntzinger E, Schmidt S, Izaurralde E, et al. The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex. Nucleic Acids Res. 2012;40:11058-72 pubmed publisher
    ..a catalytic module comprising two deadenylases (CAF1/POP2 and CCR4a/b) and the NOT module, which contains at least NOT1, NOT2 and NOT3...
  2. Sgromo A, Raisch T, Bawankar P, Bhandari D, Chen Y, Kuzuoğlu Öztürk D, et al. A CAF40-binding motif facilitates recruitment of the CCR4-NOT complex to mRNAs targeted by Drosophila Roquin. Nat Commun. 2017;8:14307 pubmed publisher
    ..and Roquin2 are RNA-binding proteins that promote mRNA target degradation through the recruitment of the CCR4-NOT deadenylase complex and are implicated in the prevention of autoimmunity...
  3. Morgunova V, Akulenko N, Radion E, Olovnikov I, Abramov Y, Olenina L, et al. Telomeric repeat silencing in germ cells is essential for early development in Drosophila. Nucleic Acids Res. 2015;43:8762-73 pubmed publisher
    ..A novel germline-specific function of deadenylase complex Ccr4-Not in the telomeric transcript surveillance mechanism is reported...
  4. Barišić Jäger E, Kręcioch I, Hosiner S, Antic S, Dorner S. HPat a decapping activator interacting with the miRNA effector complex. PLoS ONE. 2013;8:e71860 pubmed publisher
    ..The CCR4-NOT1 complex required for the deadenylation of target mRNAs is directly recruited to the miRNA effector complex...
  5. Okada H, Schittenhelm R, Straessle A, Hafen E. Multi-functional regulation of 4E-BP gene expression by the Ccr4-Not complex. PLoS ONE. 2015;10:e0113902 pubmed publisher
    ..RNAi knockdown of Not1, a putative scaffold protein of this protein complex, increases the mRNA level of 4E-BP in Drosophila Kc cells...
  6. Antic S, Wolfinger M, Skucha A, Hosiner S, Dorner S. General and MicroRNA-Mediated mRNA Degradation Occurs on Ribosome Complexes in Drosophila Cells. Mol Cell Biol. 2015;35:2309-20 pubmed publisher
    ..While it is well established that translation and mRNA degradation are tightly coupled, it is still not entirely clear where in the cell mRNA degradation takes place...
  7. Fu Z, Geng C, Wang H, Yang Z, Weng C, Li H, et al. Twin Promotes the Maintenance and Differentiation of Germline Stem Cell Lineage through Modulation of Multiple Pathways. Cell Rep. 2015;13:1366-1379 pubmed publisher
    ..Twin/CCR4 is one of the two catalytic subunits in the highly conserved CCR4-NOT mRNA deadenylase complex...
  8. Kuzuoğlu Öztürk D, Bhandari D, Huntzinger E, Fauser M, Helms S, Izaurralde E. miRISC and the CCR4-NOT complex silence mRNA targets independently of 43S ribosomal scanning. EMBO J. 2016;35:1186-203 pubmed publisher
    ..silencing suggests that AGOs mediate these effects through the sequential recruitment of GW182 proteins, the CCR4-NOT deadenylase complex and the translational repressor and decapping activator DDX6...
  9. Zekri L, Kuzuoğlu Öztürk D, Izaurralde E. GW182 proteins cause PABP dissociation from silenced miRNA targets in the absence of deadenylation. EMBO J. 2013;32:1052-65 pubmed publisher
    ..PABP dissociation requires the interaction of GW182 proteins with the CCR4-NOT complex. Accordingly, NOT1 and POP2 cause dissociation of PABP from bound mRNAs in the absence of deadenylation...
  10. Joly W, Chartier A, Rojas Ríos P, Busseau I, Simonelig M. The CCR4 deadenylase acts with Nanos and Pumilio in the fine-tuning of Mei-P26 expression to promote germline stem cell self-renewal. Stem Cell Reports. 2013;1:411-24 pubmed publisher
    ..These results identify the molecular mechanism of Nos/Pum function in GSC self-renewal and reveal the role of CCR4-NOT-mediated deadenylation in regulating the balance between GSC self-renewal and differentiation.
  11. Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E. NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT complex that docks onto the NOT1 N-terminal domain. RNA Biol. 2013;10:228-44 pubmed publisher
    ..of the complex is assembled by the interaction of at least two modules: the NOT module, which minimally consists of NOT1, NOT2 and NOT3, and a catalytic module comprising two deadenylases, CCR4 and POP2/CAF1...
  12. Raisch T, Bhandari D, Sabath K, Helms S, Valkov E, Weichenrieder O, et al. Distinct modes of recruitment of the CCR4-NOT complex by Drosophila and vertebrate Nanos. EMBO J. 2016;35:974-90 pubmed publisher
    ..In vertebrates, Nanos proteins interact with the NOT1 subunit of the CCR4-NOT effector complex through a NOT1 interacting motif (NIM), which is absent in Nanos orthologs ..
  13. Igreja C, Izaurralde E. CUP promotes deadenylation and inhibits decapping of mRNA targets. Genes Dev. 2011;25:1955-67 pubmed publisher
    ..This domain associates with the deadenylase complex CAF1-CCR4-NOT and decapping activators...