NELF-B

Summary

Gene Symbol: NELF-B
Description: NELF-B
Alias: BcDNA:GH10333, CG12152, CG32721, Dmel\CG32721, NELF, NELF-B, CG32721-PA, NELF-B-PA
Species: fruit fly

Top Publications

  1. Loppin B, Bonnefoy E, Anselme C, Laurencon A, Karr T, Couble P. The histone H3.3 chaperone HIRA is essential for chromatin assembly in the male pronucleus. Nature. 2005;437:1386-90 pubmed
    ..3 represents a primary epigenetic distinction between parental genomes in the zygote, and underlines an important consequence of the critical and highly specialized function of HIRA at fertilization. ..
  2. Wu C, Lee C, Fan R, Smith M, Yamaguchi Y, Handa H, et al. Molecular characterization of Drosophila NELF. Nucleic Acids Res. 2005;33:1269-79 pubmed
    b>NELF and DSIF act together to inhibit transcription elongation in vitro, and are implicated in causing promoter proximal pausing on the hsp70 gene in Drosophila. Here, further characterization of Drosophila NELF is provided...
  3. Lee C, Li X, Hechmer A, Eisen M, Biggin M, Venters B, et al. NELF and GAGA factor are linked to promoter-proximal pausing at many genes in Drosophila. Mol Cell Biol. 2008;28:3290-300 pubmed publisher
    ..b>NELF causes Pol II to pause in the promoter-proximal region of the hsp70 gene in Drosophila melanogaster...
  4. Adelman K, Kennedy M, Nechaev S, Gilchrist D, Muse G, Chinenov Y, et al. Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling. Proc Natl Acad Sci U S A. 2009;106:18207-12 pubmed publisher
    ..but stalls near the promoter until LPS triggers rapid and transient release of the negative elongation factor (NELF) complex and productive elongation...
  5. Wang X, Hang S, Prazak L, Gergen J. NELF potentiates gene transcription in the Drosophila embryo. PLoS ONE. 2010;5:e11498 pubmed publisher
    ..subject to developmental regulation of transcriptional elongation is association of the negative elongation factor NELF with the paused RNA polymerase complex...
  6. Gilchrist D, dos Santos G, Fargo D, Xie B, Gao Y, Li L, et al. Pausing of RNA polymerase II disrupts DNA-specified nucleosome organization to enable precise gene regulation. Cell. 2010;143:540-51 pubmed publisher
  7. Core L, Waterfall J, Gilchrist D, Fargo D, Kwak H, Adelman K, et al. Defining the status of RNA polymerase at promoters. Cell Rep. 2012;2:1025-35 pubmed publisher
    ..are inhibited from further elongation by detergent-sensitive factors, and knockdown of negative elongation factor, NELF, reduces their levels...
  8. Jennings B. Pausing for thought: disrupting the early transcription elongation checkpoint leads to developmental defects and tumourigenesis. Bioessays. 2013;35:553-60 pubmed publisher
    ..Analysis of these mutations has revealed that control of transcriptional pausing is critical for a diverse range of biological pathways essential for animal development and survival. ..
  9. Muse G, Gilchrist D, Nechaev S, Shah R, Parker J, Grissom S, et al. RNA polymerase is poised for activation across the genome. Nat Genet. 2007;39:1507-11 pubmed
    ..This finding indicates a role for regulation of polymerase elongation in the transcriptional responses to dynamic environmental and developmental cues. ..

More Information

Publications16

  1. Buscaino A, Legube G, Akhtar A. X-chromosome targeting and dosage compensation are mediated by distinct domains in MSL-3. EMBO Rep. 2006;7:531-8 pubmed
    ..Our results demonstrate that MSL-3 localization to the male X chromosome and transcriptional upregulation of X-linked genes are two separable functions of the MSL-3 protein. ..
  2. Harvey R, Schuster E, Jennings B. Pleiohomeotic interacts with the core transcription elongation factor Spt5 to regulate gene expression in Drosophila. PLoS ONE. 2013;8:e70184 pubmed publisher
    ..Our results indicate that Pho can interact with Spt5 to regulate transcription elongation in a gene specific manner. ..
  3. Zhou J, Yu W, Hardin P. ChIPping away at the Drosophila clock. Methods Enzymol. 2015;551:323-47 pubmed publisher
    ..ChIP analysis has revealed the relationship between clock factor binding, transcription, and chromatin modifications and promises to reveal circadian transcription networks that control phase and tissue specificity. ..
  4. Missra A, Gilmour D. Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex. Proc Natl Acad Sci U S A. 2010;107:11301-6 pubmed publisher
    Negative elongation factor (NELF) and 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) are involved in pausing RNA Polymerase II (Pol II) in the promoter-proximal region of the hsp70 gene in Drosophila, ..
  5. Fay A, Misulovin Z, Li J, Schaaf C, Gause M, Gilmour D, et al. Cohesin selectively binds and regulates genes with paused RNA polymerase. Curr Biol. 2011;21:1624-34 pubmed publisher
    ..Cohesin depletion does not reduce polymerase pausing, in contrast to depletion of the NELF (negative elongation factor) pausing complex...
  6. Li J, Liu Y, Rhee H, Ghosh S, Bai L, Pugh B, et al. Kinetic competition between elongation rate and binding of NELF controls promoter-proximal pausing. Mol Cell. 2013;50:711-22 pubmed publisher
    ..The location of the pause is influenced by the timing between when NELF loads onto Pol II and how fast Pol II escapes the promoter region...
  7. Li J, Gilmour D. Reconstitution of factor-dependent, promoter proximal pausing in Drosophila nuclear extracts. Methods Mol Biol. 2015;1276:133-52 pubmed publisher
    ..The radiolabeled RNAs are isolated by hybridization to a biotinylated oligonucleotide and captured on magnetic beads. We also describe the use of antibodies to investigate mechanistic aspects of promoter proximal pausing. ..