Gene Symbol: Nelf-A
Description: Negative elongation factor A
Alias: BcDNA:SD07139, CG5874, Dmel\CG5874, NELF, NELF-A, negative elongation factor A, CG5874-PA, CG5874-PB, CG5874-PC, NELF-A, Nelf-A-PA, Nelf-A-PB, Nelf-A-PC
Species: fruit fly
Wu C, Lee C, Fan R, Smith M, Yamaguchi Y, Handa H, et al
. Molecular characterization of Drosophila NELF. Nucleic Acids Res. 2005;33:1269-79 pubmed
b>NELF and DSIF act together to inhibit transcription elongation in vitro, and are implicated in causing promoter proximal pausing on the hsp70 gene in Drosophila. Here, further characterization of Drosophila NELF is provided...
Wang X, Hang S, Prazak L, Gergen J. NELF potentiates gene transcription in the Drosophila embryo. PLoS ONE. 2010;5:e11498 pubmed publisher
..subject to developmental regulation of transcriptional elongation is association of the negative elongation factor NELF with the paused RNA polymerase complex...
Fay A, Misulovin Z, Li J, Schaaf C, Gause M, Gilmour D, et al
. Cohesin selectively binds and regulates genes with paused RNA polymerase. Curr Biol. 2011;21:1624-34 pubmed publisher
..Cohesin depletion does not reduce polymerase pausing, in contrast to depletion of the NELF (negative elongation factor) pausing complex...
Takahashi A, Takahashi K, Ueda R, Takano Shimizu T. Natural variation of ebony gene controlling thoracic pigmentation in Drosophila melanogaster. Genetics. 2007;177:1233-7 pubmed
..It was found to be the difference in expression level of ebony, which codes for an enzyme in the melanin-synthesis pathway and has pleiotropic effects on vision and behavior. ..
Chopra V, Cande J, Hong J, Levine M. Stalled Hox promoters as chromosomal boundaries. Genes Dev. 2009;23:1505-9 pubmed publisher
..are dependent on general transcription factors that inhibit Pol II elongation, including components of the DSIF and NELF complexes...
Missra A, Gilmour D. Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex. Proc Natl Acad Sci U S A. 2010;107:11301-6 pubmed publisher
Negative elongation factor (NELF) and 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) are involved in pausing RNA Polymerase II (Pol II) in the promoter-proximal region of the hsp70 gene in Drosophila, ..
Jennings B. Pausing for thought: disrupting the early transcription elongation checkpoint leads to developmental defects and tumourigenesis. Bioessays. 2013;35:553-60 pubmed publisher
..Analysis of these mutations has revealed that control of transcriptional pausing is critical for a diverse range of biological pathways essential for animal development and survival. ..
Tsai S, Chang Y, Swamy K, Chiang R, Huang D. GAGA factor, a positive regulator of global gene expression, modulates transcriptional pausing and organization of upstream nucleosomes. Epigenetics Chromatin. 2016;9:32 pubmed publisher
..we detect significant genetic interactions between GAF and the nucleosome remodeler NURF, the pausing factor NELF, and BAB1 whose binding sites are enriched specifically in genes displaying GAF-dependent pausing...