Gene Symbol: Ndc80
Description: Ndc80
Alias: CG9938, Dmel\CG9938, Hec1, NDC80, dNdc80, dmNdc80, hec1, Ndc80, CG9938-PA, Hec1, Ndc80-PA
Species: fruit fly

Top Publications

  1. Cane S, Ye A, Luks Morgan S, Maresca T. Elevated polar ejection forces stabilize kinetochore-microtubule attachments. J Cell Biol. 2013;200:203-18 pubmed publisher
    ..Thus, kt-MT attachment stability is modulated by PEFs, which can be generated by distinct force-producing interactions between chromosomes and dynamic spindle microtubules...
  2. Przewloka M, Venkei Z, Bolanos Garcia V, Debski J, Dadlez M, Glover D. CENP-C is a structural platform for kinetochore assembly. Curr Biol. 2011;21:399-405 pubmed publisher
    ..We now find that all kinetochore proteins belonging to the KMN (KNL1/Spc105, the Mis12 complex, and the Ndc80 complex) network bind to the N-terminal part of Drosophila CENP-C...
  3. Schittenhelm R, Heeger S, Althoff F, Walter A, Heidmann S, Mechtler K, et al. Spatial organization of a ubiquitous eukaryotic kinetochore protein network in Drosophila chromosomes. Chromosoma. 2007;116:385-402 pubmed
    ..most diverged orthologs identified so far, which encode components of a kinetochore protein network including the Ndc80 and Mis complexes, further emphasizes the notion of a shared eukaryotic kinetochore design...
  4. Maresca T, Salmon E. Intrakinetochore stretch is associated with changes in kinetochore phosphorylation and spindle assembly checkpoint activity. J Cell Biol. 2009;184:373-81 pubmed publisher
    ..SAC regulation, we developed a Drosophila melanogaster S2 cell line expressing centromere identifier-mCherry and Ndc80-green fluorescent protein to mark the inner and outer kinetochore domains, respectively...
  5. Schittenhelm R, Chaleckis R, Lehner C. Intrakinetochore localization and essential functional domains of Drosophila Spc105. EMBO J. 2009;28:2374-86 pubmed publisher
    ..in different eukaryotes) is a comparatively large kinetochore protein, which can bind to the Mis12/MIND and Ndc80 complexes and to the spindle assembly checkpoint components Bub1 and BubR1...
  6. Furuyama T, Henikoff S. Centromeric nucleosomes induce positive DNA supercoils. Cell. 2009;138:104-13 pubmed publisher
    ..The mutual incompatibility of nucleosomes with opposite topologies could explain how centromeres are efficiently maintained as unique loci on chromosomes. ..
  7. Schittenhelm R, Althoff F, Heidmann S, Lehner C. Detrimental incorporation of excess Cenp-A/Cid and Cenp-C into Drosophila centromeres is prevented by limiting amounts of the bridging factor Cal1. J Cell Sci. 2010;123:3768-79 pubmed publisher
    ..We demonstrate that the low amount of endogenous Cal1 prevents centromere expansion and mitotic kinetochore failure when Cenp-A/Cid and Cenp-C are present in excess. ..
  8. Venkei Z, Przewloka M, Glover D. Drosophila Mis12 complex acts as a single functional unit essential for anaphase chromosome movement and a robust spindle assembly checkpoint. Genetics. 2011;187:131-40 pubmed publisher
    ..While kinetochore association of Ndc80 absolutely depends on both Mis12 and Nsl1, BubR1 localization shows only partial dependency...
  9. Meraldi P, McAinsh A, Rheinbay E, Sorger P. Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins. Genome Biol. 2006;7:R23 pubmed
    ..kinetochores constructed around Spc105 and three conserved multi-protein linker complexes (MIND, COMA, and the NDC80 complex)...

More Information


  1. Mendiburo M, Padeken J, Fülöp S, Schepers A, Heun P. Drosophila CENH3 is sufficient for centromere formation. Science. 2011;334:686-90 pubmed publisher
    ..We conclude that CID is both necessary and sufficient to serve as an epigenetic centromere mark and nucleate heritable centromere function...
  2. Morelli E, Mastrodonato V, Beznoussenko G, Mironov A, Tognon E, Vaccari T. An essential step of kinetochore formation controlled by the SNARE protein Snap29. EMBO J. 2016;35:2223-2237 pubmed
    ..Such mutant causes ectopic Knl1 recruitment to trafficking compartments. We propose that part of the outer kinetochore is functionally similar to membrane fusion interfaces. ..
  3. Radford S, Hoang T, GÅ‚uszek A, Ohkura H, McKim K. Lateral and End-On Kinetochore Attachments Are Coordinated to Achieve Bi-orientation in Drosophila Oocytes. PLoS Genet. 2015;11:e1005605 pubmed publisher
    ..We have examined oocytes lacking two kinetochore proteins, NDC80 and SPC105R, and a centromere-associated motor protein, CENP-E, to characterize the impact of kinetochore-..
  4. Lipinszki Z, Lefevre S, Savoian M, Singleton M, Glover D, Przewloka M. Centromeric binding and activity of Protein Phosphatase 4. Nat Commun. 2015;6:5894 pubmed publisher
    ..We also show that binding of Flfl to CENP-C is required to bring PP4 activity to centromeres to maintain CENP-C and attached core kinetochore proteins at chromosomes during mitosis. ..
  5. Chen C, Dechassa M, Bettini E, Ledoux M, Belisario C, Heun P, et al. CAL1 is the Drosophila CENP-A assembly factor. J Cell Biol. 2014;204:313-29 pubmed publisher
    ..Collectively, this work identifies the "missing" CENP-A chaperone in flies, revealing fundamental conservation between insect and vertebrate centromere-specification mechanisms. ..
  6. Moutinho Santos T, Conde C, Sunkel C. POLO ensures chromosome bi-orientation by preventing and correcting erroneous chromosome-spindle attachments. J Cell Sci. 2012;125:576-83 pubmed publisher
    ..Our results suggests that POLO is required to promote amphitelic attachment and chromosome bi-orientation by regulating both the activity of the correction mechanism and the architecture of the centromere. ..
  7. Meireles A, Dzhindzhev N, Ohkura H. Kebab: kinetochore and EB1 associated basic protein that dynamically changes its localisation during Drosophila mitosis. PLoS ONE. 2011;6:e24174 pubmed publisher
    ..The protein has a domain most similar to the atypical CH domain of Ndc80, and a coiled-coil domain...
  8. Venkei Z, Przewloka M, Ladak Y, Albadri S, Sossick A, Juhasz G, et al. Spatiotemporal dynamics of Spc105 regulates the assembly of the Drosophila kinetochore. Open Biol. 2012;2:110032 pubmed publisher
    ..The cumulative kinetochore levels of Spc105 and Mis12 complex then determine the rate of Ndc80 complex recruitment commencing only after nuclear envelope breakdown...
  9. Conde C, Osswald M, Barbosa J, Moutinho Santos T, Pinheiro D, Guimaraes S, et al. Drosophila Polo regulates the spindle assembly checkpoint through Mps1-dependent BubR1 phosphorylation. EMBO J. 2013;32:1761-77 pubmed publisher
    ..We propose a model in which Polo controls Mps1-dependent BubR1 phosphorylation to promote Cdc20 kinetochore recruitment and sustained SAC function. ..
  10. Cane S, McGilvray P, Maresca T. Insights from an erroneous kinetochore-microtubule attachment state. Bioarchitecture. 2013;3:69-76 pubmed publisher
    ..Our force-related studies are also discussed in the context of current models for tension-dependent stabilization of kt-MT interactions. ..
  11. Bucciarelli E, Pellacani C, Naim V, Palena A, Gatti M, Somma M. Drosophila Dgt6 interacts with Ndc80, Msps/XMAP215, and gamma-tubulin to promote kinetochore-driven MT formation. Curr Biol. 2009;19:1839-45 pubmed publisher
    ..Consistent with these results, Dgt6 coprecipitates with Msps, D-TACC, gamma-tubulin, Ndc80, and Nuf2...
  12. White G, Erickson H. The coiled coils of cohesin are conserved in animals, but not in yeast. PLoS ONE. 2009;4:e4674 pubmed publisher
    ..However, in yeast they are no more constrained than the coils of SMC2/4 and Ndc80/Nuf2p, suggesting that they are serving primarily as spacer rods...
  13. Williams B, Leung G, Maiato H, Wong A, Li Z, Williams E, et al. Mitch a rapidly evolving component of the Ndc80 kinetochore complex required for correct chromosome segregation in Drosophila. J Cell Sci. 2007;120:3522-33 pubmed
    ..unit CG7242, and encodes a protein that is a possible ortholog of the Spc24 or Spc25 subunit of the Ndc80 kinetochore complex...
  14. Olszak A, van Essen D, Pereira A, Diehl S, Manke T, Maiato H, et al. Heterochromatin boundaries are hotspots for de novo kinetochore formation. Nat Cell Biol. 2011;13:799-808 pubmed publisher
    ..These data indicate that the characteristics of regions bordering heterochromatin promote de novo kinetochore assembly and thereby contribute to centromere identity. ..
  15. Savoian M, Glover D. Drosophila Klp67A binds prophase kinetochores to subsequently regulate congression and spindle length. J Cell Sci. 2010;123:767-76 pubmed publisher
    ..From this location, Klp67A uses its motor activity to ensure chromosome alignment and proper spindle length. ..
  16. Feijão T, Afonso O, Maia A, Sunkel C. Stability of kinetochore-microtubule attachment and the role of different KMN network components in Drosophila. Cytoskeleton (Hoboken). 2013;70:661-75 pubmed publisher
    ..super-complex of proteins, known as the KNL1Mis12Ndc80 (KMN) network, composed by the KNL1 (Spc105), Mis12, and Ndc80 complexes...
  17. Lince Faria M, Maffini S, Orr B, Ding Y, Cláudia Florindo -, Sunkel C, et al. Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator. J Cell Biol. 2009;184:647-57 pubmed publisher
  18. Drpic D, Pereira A, Barisic M, Maresca T, Maiato H. Polar Ejection Forces Promote the Conversion from Lateral to End-on Kinetochore-Microtubule Attachments on Mono-oriented Chromosomes. Cell Rep. 2015;13:460-468 pubmed publisher
    ..Thus, PEFs convert lateral to stable end-on kinetochore-microtubule attachments, independently of chromosome bi-orientation. ..