Gene Symbol: Mst77F
Description: Male-specific transcript 77F
Alias: 18c, CG3354, Dmel\CG3354, MST77F, R1-18c, ms(3)nc3, mst77F, nc3, Male-specific transcript 77F, CG3354-PA, Mst77F-PA, male sterile (3) nc3
Species: fruit fly

Top Publications

  1. Russell S, Kaiser K. Drosophila melanogaster male germ line-specific transcripts with autosomal and Y-linked genes. Genetics. 1993;134:293-308 pubmed
    ..The autosomal locus, at 77F on chromosome arm 3L, corresponds to the previously described transcription unit 18c, located in the first intron of the gene for an RI subunit of cAMP-dependent protein kinase...
  2. Jayaramaiah Raja S, Renkawitz Pohl R. Replacement by Drosophila melanogaster protamines and Mst77F of histones during chromatin condensation in late spermatids and role of sesame in the removal of these proteins from the male pronucleus. Mol Cell Biol. 2005;25:6165-77 pubmed
    ..Here we characterize three genes in the fly genome, Mst35Ba, Mst35Bb, and Mst77F. The results indicate that Mst35Ba and Mst35Bb encode dProtA and dProtB, respectively...
  3. Krsticevic F, Santos H, Januário S, Schrago C, Carvalho A. Functional copies of the Mst77F gene on the Y chromosome of Drosophila melanogaster. Genetics. 2010;184:295-307 pubmed publisher
    ..In 1993, Russell and Kaiser found three Y-linked pseudogenes of the Mst77F gene, which is a testis-expressed autosomal gene that is essential for male fertility...
  4. Rathke C, Barckmann B, Burkhard S, Jayaramaiah Raja S, Roote J, Renkawitz Pohl R. Distinct functions of Mst77F and protamines in nuclear shaping and chromatin condensation during Drosophila spermiogenesis. Eur J Cell Biol. 2010;89:326-38 pubmed publisher
    ..In Drosophila, we previously identified protamine A, protamine B and Mst77F as major chromatin components of the mature sperm...
  5. Kalderon D, Rubin G. Isolation and characterization of Drosophila cAMP-dependent protein kinase genes. Genes Dev. 1988;2:1539-56 pubmed
    ..The remaining putative gene products include amino acid sequence motifs characteristic of serine-threonine protein kinases but cannot, from the available data, be defined as homologous to specific protein kinases of other organisms. ..
  6. Loppin B, Dubruille R, Horard B. The intimate genetics of Drosophila fertilization. Open Biol. 2015;5: pubmed publisher
  7. Dubruille R, Loppin B. Protection of Drosophila chromosome ends through minimal telomere capping. J Cell Sci. 2015;128:1969-81 pubmed publisher
    ..We propose that these complexes prevent fusions by acting at the very extremity of chromosomes, reminiscent of the protection conferred by extremely short telomeric arrays in yeast or mammalian systems. ..
  8. Barckmann B, Chen X, Kaiser S, Jayaramaiah Raja S, Rathke C, Dottermusch Heidel C, et al. Three levels of regulation lead to protamine and Mst77F expression in Drosophila. Dev Biol. 2013;377:33-45 pubmed publisher
    ..Here we present evidence that expression of Mst77F, which is a specialized linker histone-like component of sperm chromatin, and of protamine B (ProtB), which ..
  9. Gärtner S, Rothenbusch S, Buxa M, Theofel I, Renkawitz R, Rathke C, et al. The HMG-box-containing proteins tHMG-1 and tHMG-2 interact during the histone-to-protamine transition in Drosophila spermatogenesis. Eur J Cell Biol. 2015;94:46-59 pubmed publisher
    ..We hypothesize that the mechanism of chromatin reorganization is a process highly secured by redundancies. ..

More Information


  1. Francisco F, Lemos B. How do y-chromosomes modulate genome-wide epigenetic States: genome folding, chromatin sinks, and gene expression. J Genomics. 2014;2:94-103 pubmed publisher
    ..We also discuss how the discovery of this new function impacts the current knowledge about Y chromosome origin, its current dynamics, and future fate. ..
  2. Dubruille R, Orsi G, Delabaere L, Cortier E, Couble P, Marais G, et al. Specialization of a Drosophila capping protein essential for the protection of sperm telomeres. Curr Biol. 2010;20:2090-9 pubmed publisher
    ..We propose that K81 specialized in the maintenance of telomere protection in the highly peculiar chromatin environment of differentiating male gametes...
  3. Balakirev E, Ayala F. Pseudogenes: are they "junk" or functional DNA?. Annu Rev Genet. 2003;37:123-51 pubmed publisher
    ..We agree with the proposal that pseudogenes be considered as potogenes, i.e., DNA sequences with a potentiality for becoming new genes...
  4. Gatti M, Pimpinelli S. Functional elements in Drosophila melanogaster heterochromatin. Annu Rev Genet. 1992;26:239-75 pubmed
  5. Leser K, Awe S, Barckmann B, Renkawitz Pohl R, Rathke C. The bromodomain-containing protein tBRD-1 is specifically expressed in spermatocytes and is essential for male fertility. Biol Open. 2012;1:597-606 pubmed publisher
    ..In Drosophila, the mRNAs of proteins required for post-meiotic chromatin reorganisation, like ProtB and Mst77F, are transcribed in meiotic spermatocytes and subjected to translational repression for days...
  6. Tirmarche S, Kimura S, Sapey Triomphe L, Sullivan W, Landmann F, Loppin B. Drosophila protamine-like Mst35Ba and Mst35Bb are required for proper sperm nuclear morphology but are dispensable for male fertility. G3 (Bethesda). 2014;4:2241-5 pubmed publisher
    ..Here, we have precisely deleted the Mst35B locus by homologous recombination, and we confirm the dispensability of Mst35Ba/b for fertility. ..
  7. Vardanyan A, Atanesyan L, Egli D, Raja S, Steinmann Zwicky M, Renkawitz Pohl R, et al. Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in Drosophila. BMC Dev Biol. 2008;8:68 pubmed publisher
    ..The results from these studies reveal an inhibitory effect of Dpy-30L1 on MTF-1 and an essential role for Dpy-30L2 in male fertility. ..
  8. Kost N, Kaiser S, Ostwal Y, Riedel D, Stützer A, Nikolov M, et al. Multimerization of Drosophila sperm protein Mst77F causes a unique condensed chromatin structure. Nucleic Acids Res. 2015;43:3033-45 pubmed publisher
    ..Therefore, we analyzed the interaction of Mst77F, another very basic testis-specific protein with chromatin and DNA as well as studied the molecular consequences ..
  9. Osoegawa K, Vessere G, Li Shu C, Hoskins R, Abad J, de Pablos B, et al. BAC clones generated from sheared DNA. Genomics. 2007;89:291-9 pubmed
    ..The utility of "sheared" libraries was demonstrated by closure of a previous clone gap and by isolation of clones from telomeric regions, which were notably absent from previous Drosophila BAC libraries. ..
  10. Eren Ghiani Z, Rathke C, Theofel I, Renkawitz Pohl R. Prtl99C Acts Together with Protamines and Safeguards Male Fertility in Drosophila. Cell Rep. 2015;13:2327-2335 pubmed publisher
    ..These data reveal that at least three chromatin-binding proteins act together in chromatin reorganization to compact the paternal chromatin. ..
  11. Kimura S, Loppin B. The Drosophila chromosomal protein Mst77F is processed to generate an essential component of mature sperm chromatin. Open Biol. 2016;6: pubmed
    ..sperm DNA to reach an extreme level of compaction, but little is known about how SNBPs actually function in vivo Mst77F is a Drosophila SNBP with unique DNA condensation properties in vitro, but its role during spermiogenesis remains ..
  12. Doyen C, Moshkin Y, Chalkley G, Bezstarosti K, Demmers J, Rathke C, et al. Subunits of the histone chaperone CAF1 also mediate assembly of protamine-based chromatin. Cell Rep. 2013;4:59-65 pubmed publisher
    ..These results show that CAF1 subunits mediate the assembly of two fundamentally different forms of chromatin. ..