Gene Symbol: mei-S332
Description: meiotic from via Salaria 332
Alias: BEST:LD13774, CG5303, Dmel\CG5303, MEI-S322, MEI-S332, Mei-S332, Mei-S332/Shugoshin, MeiS332, mei-S322, mei-s332, meiS322, meiS332, meiotic from via salaria 332, CG5303-PA, CG5303-PB, MeiS332, mei-S332-PA, mei-S332-PB, meiotic from salaria 332, shugoshin
Species: fruit fly

Top Publications

  1. Miyazaki W, Orr Weaver T. Sister-chromatid misbehavior in Drosophila ord mutants. Genetics. 1992;132:1047-61 pubmed
    ..The ord gene product may prevent premature sister-chromatid separation by promoting cohesion of the sister chromatids in a structural or regulatory manner. ..
  2. Balicky E, Endres M, Lai C, Bickel S. Meiotic cohesion requires accumulation of ORD on chromosomes before condensation. Mol Biol Cell. 2002;13:3890-900 pubmed
  3. Clarke A, Tang T, Ooi D, Orr Weaver T. POLO kinase regulates the Drosophila centromere cohesion protein MEI-S332. Dev Cell. 2005;8:53-64 pubmed
    ..POLO phosphorylates MEI-S332 in vitro, POLO and MEI-S332 bind each other, and mutation of POLO binding sites prevents MEI-S332 dissociation from centromeres. ..
  4. Bickel S, Wyman D, Orr Weaver T. Mutational analysis of the Drosophila sister-chromatid cohesion protein ORD and its role in the maintenance of centromeric cohesion. Genetics. 1997;146:1319-31 pubmed
    ..Therefore, ORD activity appears to promote centromeric cohesion during meiosis II but is not essential for kinetochore function during anaphase. ..
  5. Yan R, Thomas S, Tsai J, Yamada Y, McKee B. SOLO: a meiotic protein required for centromere cohesion, coorientation, and SMC1 localization in Drosophila melanogaster. J Cell Biol. 2010;188:335-49 pubmed publisher anaphase I in mutants for mei-S332, which encodes the Drosophila homologue of the cohesin protector protein shugoshin. The solo mutant phenotypes and the localization patterns of SOLO and SMC1 indicate that they function together ..
  6. Moore D, Page A, Tang T, Kerrebrock A, Orr Weaver T. The cohesion protein MEI-S332 localizes to condensed meiotic and mitotic centromeres until sister chromatids separate. J Cell Biol. 1998;140:1003-12 pubmed
    ..Thus, MEI-S332 associates with the centromeres of both meiotic and mitotic chromosomes and dissociates from them at anaphase. ..
  7. LeBlanc H, Tang T, Wu J, Orr Weaver T. The mitotic centromeric protein MEI-S332 and its role in sister-chromatid cohesion. Chromosoma. 1999;108:401-11 pubmed
    ..MEI-S332 is the first member identified of a predicted class of centromeric proteins that maintain centromeric cohesion. ..
  8. Heun P, Erhardt S, Blower M, Weiss S, Skora A, Karpen G. Mislocalization of the Drosophila centromere-specific histone CID promotes formation of functional ectopic kinetochores. Dev Cell. 2006;10:303-15 pubmed
    ..Thus, CENP-A mislocalization is one possible mechanism for genome instability during cancer progression, as well as centromere plasticity during evolution. ..
  9. Resnick T, Satinover D, MacIsaac F, Stukenberg P, Earnshaw W, Orr Weaver T, et al. INCENP and Aurora B promote meiotic sister chromatid cohesion through localization of the Shugoshin MEI-S332 in Drosophila. Dev Cell. 2006;11:57-68 pubmed
    ..These results implicate the chromosomal passenger complex in directly regulating MEI-S332 localization and, therefore, the control of sister chromatid cohesion in meiosis. ..

More Information


  1. Williams B, Gatti M, Goldberg M. Bipolar spindle attachments affect redistributions of ZW10, a Drosophila centromere/kinetochore component required for accurate chromosome segregation. J Cell Biol. 1996;134:1127-40 pubmed
    ..We propose that ZW10 acts as part of, or immediately downstream of, a tension-sensing mechanism that regulates chromosome separation or movement at anaphase onset. ..
  2. Orr Weaver T. Meiosis in Drosophila: seeing is believing. Proc Natl Acad Sci U S A. 1995;92:10443-9 pubmed
    ..Additional advances in understanding Drosophila meiosis are the delineation of a functional centromere by using minichromosome derivatives and the identification of several regulatory genes for the meiotic cell cycle. ..
  3. Kerrebrock A, Miyazaki W, Birnby D, Orr Weaver T. The Drosophila mei-S332 gene promotes sister-chromatid cohesion in meiosis following kinetochore differentiation. Genetics. 1992;130:827-41 pubmed
    ..All of the alleles are fully viable when in trans to a deficiency, thus mei-S332 is not essential for mitosis. Four of the alleles show an unexpected sex specificity. ..
  4. Goldstein L. Mechanisms of chromosome orientation revealed by two meiotic mutants in Drosophila melanogaster. Chromosoma. 1980;78:79-111 pubmed
    ..Finally, functions which are required throughout meiosis in both sexes must be considered in the pathways of meiotic control. ..
  5. Petronczki M, Siomos M, Nasmyth K. Un ménage à quatre: the molecular biology of chromosome segregation in meiosis. Cell. 2003;112:423-40 pubmed
  6. Kerrebrock A, Moore D, Wu J, Orr Weaver T. Mei-S332, a Drosophila protein required for sister-chromatid cohesion, can localize to meiotic centromere regions. Cell. 1995;83:247-56 pubmed
    ..When sister chromatids separate at anaphase II, mei-S332-GFP disappears from the chromosomes, suggesting that the destruction or release of this protein is required for sister-chromatid separation. ..
  7. Smith M. Centromeres and variant histones: what, where, when and why?. Curr Opin Cell Biol. 2002;14:279-85 pubmed
    ..Finally, a new report provides a glimpse into the potential regulation of CENP-A through specific post-translational phosphorylation, suggesting a broad level of control through histone tail modifications. ..
  8. Blower M, Karpen G. The role of Drosophila CID in kinetochore formation, cell-cycle progression and heterochromatin interactions. Nat Cell Biol. 2001;3:730-9 pubmed
  9. Lopez J, Karpen G, Orr Weaver T. Sister-chromatid cohesion via MEI-S332 and kinetochore assembly are separable functions of the Drosophila centromere. Curr Biol. 2000;10:997-1000 pubmed
    ..These results suggest that in higher eukaryotes cohesion is controlled by the functional centromere, and that, in contrast to yeast [5], the requirements for cohesion are separable from those for kinetochore assembly. ..
  10. Tang T, Bickel S, Young L, Orr Weaver T. Maintenance of sister-chromatid cohesion at the centromere by the Drosophila MEI-S332 protein. Genes Dev. 1998;12:3843-56 pubmed
  11. Davis B. Genetic analysis of a meiotic mutant resulting in precocious sister-centromere separation in Drosophila melanogaster. Mol Gen Genet. 1971;113:251-72 pubmed
  12. Maddox P, Desai A, Oegema K, Mitchison T, Salmon E. Poleward microtubule flux is a major component of spindle dynamics and anaphase a in mitotic Drosophila embryos. Curr Biol. 2002;12:1670-4 pubmed
  13. Wu C, Singaram V, McKim K. mei-38 is required for chromosome segregation during meiosis in Drosophila females. Genetics. 2008;180:61-72 pubmed publisher
    ..We suggest MEI-38 is required for the stability of parallel microtubules, including the kinetochore microtubules. ..
  14. Kim J, Nordman J, Xie F, Kashevsky H, Eng T, Li S, et al. Integrative analysis of gene amplification in Drosophila follicle cells: parameters of origin activation and repression. Genes Dev. 2011;25:1384-98 pubmed publisher
    ..This repression is alleviated when a fragment containing the origin is placed in different genomic contexts. ..
  15. Baker B, Carpenter A, Ripoll P. The Utilization during Mitotic Cell Division of Loci Controlling Meiotic Recombination and Disjunction in DROSOPHILA MELANOGASTER. Genetics. 1978;90:531-78 pubmed
    ..Mutants at the two remaining loci (nod, pal) do not affect mitotic chromosome stability. ..
  16. Dej K, Ahn C, Orr Weaver T. Mutations in the Drosophila condensin subunit dCAP-G: defining the role of condensin for chromosome condensation in mitosis and gene expression in interphase. Genetics. 2004;168:895-906 pubmed
    ..We also identified a role for dCAP-G during interphase in regulating heterochromatic gene expression. ..
  17. Lee J, Dej K, Lopez J, Orr Weaver T. Control of centromere localization of the MEI-S332 cohesion protection protein. Curr Biol. 2004;14:1277-83 pubmed
    ..MEI-S332 can localize independently of cohesin, assembling even onto unreplicated chromatids. However, the separase pathway that regulates cohesin dissociation is needed for MEI-S332 delocalization at anaphase. ..
  18. Sandler L, Lindsley D, Nicoletti B, Trippa G. Mutants affecting meiosis in natural populations of Drosophila melanogaster. Genetics. 1968;60:525-58 pubmed
  19. Sibon O, Kelkar A, Lemstra W, Theurkauf W. DNA-replication/DNA-damage-dependent centrosome inactivation in Drosophila embryos. Nat Cell Biol. 2000;2:90-5 pubmed
    ..We propose that centrosome inactivation is part of a damage-control system that blocks chromosome segregation when replication/damage checkpoint control fails. ..
  20. Resnick T, Dej K, Xiang Y, Hawley R, Ahn C, Orr Weaver T. Mutations in the chromosomal passenger complex and the condensin complex differentially affect synaptonemal complex disassembly and metaphase I configuration in Drosophila female meiosis. Genetics. 2009;181:875-87 pubmed publisher
    ..The dcap-g and incenp mutations cause maternal effect lethality, with embryos from mutant mothers arrested in the initial mitotic divisions...
  21. Emberly E, Blattes R, Schuettengruber B, Hennion M, Jiang N, Hart C, et al. BEAF regulates cell-cycle genes through the controlled deposition of H3K9 methylation marks into its conserved dual-core binding sites. PLoS Biol. 2008;6:2896-910 pubmed publisher
    ..Our results reveal a novel role for BEAF chromatin dual-cores in regulating a distinct set of genes involved in chromosome organization/segregation and the cell cycle. ..
  22. Choo K. Domain organization at the centromere and neocentromere. Dev Cell. 2001;1:165-77 pubmed
    ..Studies in different organisms point to a model of conserved pattern of organization for these domains. ..
  23. Hawley R. Meiosis as an "M" thing: twenty-five years of meiotic mutants in Drosophila. Genetics. 1993;135:613-8 pubmed
  24. Valdeolmillos A, Rufas J, Suja J, Vass S, Heck M, Martinez A C, et al. Drosophila cohesins DSA1 and Drad21 persist and colocalize along the centromeric heterochromatin during mitosis. Biol Cell. 2004;96:457-62 pubmed
    ..These results provide strong evidence that DSA1 and Drad21 are partners in a cohesin complex involved in the maintenance of sister chromatid arm and centromeric cohesion during mitosis in Drosophila. ..
  25. Rangone H, Wegel E, Gatt M, Yeung E, Flowers A, Debski J, et al. Suppression of scant identifies Endos as a substrate of greatwall kinase and a negative regulator of protein phosphatase 2A in mitosis. PLoS Genet. 2011;7:e1002225 pubmed publisher
    ..Together these interactions suggest that Greatwall and Endos act to promote the inactivation of PP2A-Twins/B55 in Drosophila. We discuss the involvement of Polo kinase in such a regulatory loop. ..
  26. Radford S, Hoang T, GÅ‚uszek A, Ohkura H, McKim K. Lateral and End-On Kinetochore Attachments Are Coordinated to Achieve Bi-orientation in Drosophila Oocytes. PLoS Genet. 2015;11:e1005605 pubmed publisher
    ..Together, these results demonstrate that, in oocytes, kinetochore-dependent and -independent chromosome-microtubule attachments work together to promote the accurate segregation of chromosomes. ..
  27. Compton D. Chromosome segregation: pulling from the poles. Curr Biol. 2002;12:R651-3 pubmed
    ..The data reveal that poleward microtubule flux provides the dominant force for separating chromatids in Drosophila embryos during anaphase A. ..
  28. Orr B, Sunkel C. Drosophila CENP-C is essential for centromere identity. Chromosoma. 2011;120:83-96 pubmed publisher
    ..We suggest that CENP-C might fulfill the structural roles of the human centromere-associated proteins not identified in Drosophila. ..
  29. van Heemst D, Heyting C. Sister chromatid cohesion and recombination in meiosis. Chromosoma. 2000;109:10-26 pubmed
    ..In meiosis, cohesion and recombination are modified in such a way that reciprocal exchange and reductional segregation of homologous chromosomes are ensured. ..
  30. Dobie K, Hari K, Maggert K, Karpen G. Centromere proteins and chromosome inheritance: a complex affair. Curr Opin Genet Dev. 1999;9:206-17 pubmed
    ..A picture is beginning to emerge of the centromere-kinetechore as a complex and dynamic structure with conservation of function at the protein level across diverse species. ..
  31. Fenger D, Carminati J, Burney Sigman D, Kashevsky H, Dines J, Elfring L, et al. PAN GU: a protein kinase that inhibits S phase and promotes mitosis in early Drosophila development. Development. 2000;127:4763-74 pubmed
    ..Our results reveal a novel protein kinase complex that controls S phase at the onset of development apparently by stabilizing mitotic cyclins. ..
  32. Hardy R. The influence of chromosome content on the size and shape of sperm heads in Drosophila melanogaster and the demonstration of chromosome loss during spermiogenesis. Genetics. 1975;79:231-64 pubmed
    ..5 over nullo-X, nullo-Y sperm in leaving the seminal receptacle of the female for fertilization of ova. ..
  33. Sandler L, Romans P, Figenshow J. An effect of centromere function on the behavior of ring-X chromosomes in Drosophila melanogaster. Genetics. 1974;77:299-307 pubmed
  34. Cook K, Murphy T, Nguyen T, Karpen G. Identification of trans-acting genes necessary for centromere function in Drosophila melanogaster using centromere-defective minichromosomes. Genetics. 1997;145:737-47 pubmed
    ..The results presented in this study strongly suggest that dominant genetic interactions between mutations and centromere-defective minichromosomes could be used effectively to identify novel genes necessary for centromere function. ..
  35. Delcros J, Prigent C, Giet R. Dynactin targets Pavarotti-KLP to the central spindle during anaphase and facilitates cytokinesis in Drosophila S2 cells. J Cell Sci. 2006;119:4431-41 pubmed
    ..The contribution of the dynein-dynactin complex to synchronous chromosome segregation and cytokinesis is discussed. ..
  36. Bickel S, Moore D, Lai C, Orr Weaver T. Genetic interactions between mei-S332 and ord in the control of sister-chromatid cohesion. Genetics. 1998;150:1467-76 pubmed
    ..Our results suggest that a balance between the activity of mei-S332 and ord is required for proper regulation of meiotic cohesion and demonstrate that additional proteins must be functioning to ensure mitotic sister-chromatid cohesion. ..
  37. Liu X, Lengyel J. Drosophila arc encodes a novel adherens junction-associated PDZ domain protein required for wing and eye development. Dev Biol. 2000;221:419-34 pubmed
    ..Misexpression of arc in the eye imaginal discs results in rough and larger eyes with fused ommatidia. We propose that arc affects eye development by modulating adherens junctions of the developing ommatidium. ..
  38. Allshire R. Centromeres, checkpoints and chromatid cohesion. Curr Opin Genet Dev. 1997;7:264-73 pubmed
    ..Phenotypic and localization data combined with their pattern of rapid degradation at anaphase have implicated several yeast and Drosophila proteins in aspects of sister chromatid cohesion. ..
  39. D Avino P, Archambault V, Przewloka M, Zhang W, Laue E, Glover D. Isolation of protein complexes involved in mitosis and cytokinesis from Drosophila cultured cells. Methods Mol Biol. 2009;545:99-112 pubmed publisher
    ..Although this method has proven very successful in isolating mitotic and cytokinetic complexes, it can also be used to characterise protein complexes involved in many other cellular processes. ..
  40. Daniels S, Chovnick A. P element transposition in Drosophila melanogaster: an analysis of sister-chromatid pairs and the formation of intragenic secondary insertions during meiosis. Genetics. 1993;133:623-36 pubmed
    ..We estimated that approximately 4% of the dysgenic male gametes have transposon perturbations of meiotic origin; the proportion of gametes containing lesions of premeiotic origin was estimated at 32%. ..
  41. Sekelsky J, Hawley R. The bond between sisters. Cell. 1995;83:157-60 pubmed
  42. Stoop Myer C, Amon A. Meiosis: Rec8 is the reason for cohesion. Nat Cell Biol. 1999;1:E125-7 pubmed
  43. Biggins S, Murray A. Sister chromatid cohesion in mitosis. Curr Opin Genet Dev. 1999;9:230-6 pubmed
    ..Proteins that induce and regulate the separation of sister chromatids have also been recently identified. (This review is an updated version of one that was published in Current Opinion in Cell Biology 1998, 10:769-775.) ..
  44. Moutinho Santos T, Conde C, Sunkel C. POLO ensures chromosome bi-orientation by preventing and correcting erroneous chromosome-spindle attachments. J Cell Sci. 2012;125:576-83 pubmed publisher
    ..Our results suggests that POLO is required to promote amphitelic attachment and chromosome bi-orientation by regulating both the activity of the correction mechanism and the architecture of the centromere. ..
  45. Gethmann R. Meiosis in male Drosophila melanogaster I. Isolation and characterization of meiotic mutants affecting second chromosome disjuction. Genetics. 1974;78:1127-42 pubmed
    ..Among the double exceptions, there is an excess of XY nullo-2 and nullo-XY diplo-2 gametes. Meiotic drive, chromosome loss and nonhomologous pairing are considered as possible explanations for the double exceptions. ..
  46. Torras Llort M, Medina Giró S, Moreno Moreno O, Azorín F. A conserved arginine-rich motif within the hypervariable N-domain of Drosophila centromeric histone H3 (CenH3) mediates BubR1 recruitment. PLoS ONE. 2010;5:e13747 pubmed publisher
  47. Matthies H, Messina L, Namba R, Greer K, Walker M, Hawley R. Mutations in the alpha-tubulin 67C gene specifically impair achiasmate segregation in Drosophila melanogaster. J Cell Biol. 1999;147:1137-44 pubmed
    ..These results suggest that the accurate segregation of achiasmate chromosomes requires the proper balancing of forces acting on the chromosomes during prometaphase. ..
  48. Nogueira C, Kashevsky H, Pinto B, Clarke A, Orr Weaver T. Regulation of centromere localization of the Drosophila Shugoshin MEI-S332 and sister-chromatid cohesion in meiosis. G3 (Bethesda). 2014;4:1849-58 pubmed publisher
    The Shugoshin (Sgo) protein family helps to ensure proper chromosome segregation by protecting cohesion at the centromere by preventing cleavage of the cohesin complex...
  49. Salvany L, Aldaz S, Corsetti E, Azpiazu N. A new role for hth in the early pre-blastodermic divisions in drosophila. Cell Cycle. 2009;8:2748-55 pubmed
    ..The transcripts derived from these sequences are needed for the correct assembly of the centric heterochromatin. ..
  50. Rieder C, Cole R. Chromatid cohesion during mitosis: lessons from meiosis. J Cell Sci. 1999;112 ( Pt 16):2607-13 pubmed
    ..This finding provides a straightforward explanation for why mutants in proteins responsible for centromeric cohesion in Drosophila (e.g. ord, mei-s332) disrupt meiosis but not mitosis. ..
  51. Sharp D, Rogers G, Scholey J. Cytoplasmic dynein is required for poleward chromosome movement during mitosis in Drosophila embryos. Nat Cell Biol. 2000;2:922-30 pubmed
    ..Thus, dynein is essential for poleward chromosome motility throughout mitosis in Drosophila embryos. ..
  52. Hirai K, Toyohira S, Ohsako T, Yamamoto M. Isolation and cytogenetic characterization of male meiotic mutants of Drosophila melanogaster. Genetics. 2004;166:1795-806 pubmed
    ..mei(2)yh92 predominantly induced nondisjunction at meiosis I that appeared to be the consequence of failure of the separation of paired homologous chromosomes...
  53. Page A, Orr Weaver T. The Drosophila genes grauzone and cortex are necessary for proper female meiosis. J Cell Sci. 1996;109 ( Pt 7):1707-15 pubmed
    ..The mutations had no observable effect on either male meiosis or mitosis. We believe these genes will provide insight into the developmental regulation of meiosis in a genetically tractable organism. ..
  54. Jang J, Rahman T, McKim K. The kinesinlike protein Subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes. Mol Biol Cell. 2005;16:4684-94 pubmed
    ..We propose that Subito is required for establishing and/or maintaining the central spindle in Drosophila oocytes, and this substitutes for the role of centrosomes in organizing the bipolar spindle. ..
  55. Williams B, Garrett Engele C, Li Z, Williams E, Rosenman E, Goldberg M. Two putative acetyltransferases, san and deco, are required for establishing sister chromatid cohesion in Drosophila. Curr Biol. 2003;13:2025-36 pubmed
    ..At least two diverse acetyltransferases play vital roles in regulating sister chromatid cohesion during Drosophila mitosis. ..
  56. Philip A. Mitotic sister-chromatid separation: what Drosophila mutants can tell us. Trends Cell Biol. 1998;8:150 pubmed
  57. Oliveira R, Kotadia S, Tavares A, Mirkovic M, Bowlin K, Eichinger C, et al. Centromere-independent accumulation of cohesin at ectopic heterochromatin sites induces chromosome stretching during anaphase. PLoS Biol. 2014;12:e1001962 pubmed publisher
  58. Lake C, Hawley R. A new target for POLO in meiotic centromere cohesion. Dev Cell. 2005;8:5-7 pubmed
    ..In this issue of Developmental Cell, Clarke et al. show that POLO also regulates the function of the MEI-S332 protein, which plays a critical role in the maintenance of sister chromatid cohesion at the centromere during meiosis. ..