Gene Symbol: JhI-1
Description: Juvenile hormone-inducible protein 1
Alias: CG3298, Dmel\CG3298, JHI-1, RNZ, dRNase Z, dRNaseZ, dtRNaseZ, jhI-1, tRNAse Z, tRNase Z, juvenile hormone-inducible protein 1, CG3298-PB, JhI-1-PB, tRNase Z
Species: fruit fly

Top Publications

  1. Zareen N, Yan H, Hopkinson A, Levinger L. Residues in the conserved His domain of fruit fly tRNase Z that function in catalysis are not involved in substrate recognition or binding. J Mol Biol. 2005;350:189-99 pubmed
    ..RNase P removes endonucleolytically the 5' end leader. tRNase Z can remove endonucleolytically the 3' end trailer as a necessary step in tRNA maturation...
  2. Zareen N, Hopkinson A, Levinger L. Residues in two homology blocks on the amino side of the tRNase Z His domain contribute unexpectedly to pre-tRNA 3' end processing. RNA. 2006;12:1104-15 pubmed
    b>tRNase Z, which can endonucleolytically remove pre-tRNA 3'-end trailers, possesses the signature His domain (HxHxDH; Motif II) of the beta-lactamase family of metal-dependent hydrolases...
  3. Levinger L, Hopkinson A, Desetty R, Wilson C. Effect of changes in the flexible arm on tRNase Z processing kinetics. J Biol Chem. 2009;284:15685-91 pubmed publisher
    ..Central to tRNA maturation, the 3' end trailer can be endonucleolytically removed by tRNase Z. A flexible arm (FA) extruded from the body of tRNase Z consists of a structured alphaalphabetabeta hand that ..
  4. Yang X, Burch B, Yan Y, Marzluff W, Dominski Z. FLASH, a proapoptotic protein involved in activation of caspase-8, is essential for 3' end processing of histone pre-mRNAs. Mol Cell. 2009;36:267-78 pubmed publisher
    ..These results demonstrate that FLASH is an essential factor required for 3' end maturation of histone mRNAs in both vertebrates and invertebrates and suggest a potential link between this process and apoptosis. ..
  5. Xie X, Dubrovsky E. Knockout of Drosophila RNase ZL impairs mitochondrial transcript processing, respiration and cell cycle progression. Nucleic Acids Res. 2015;43:10364-75 pubmed publisher
    ..Similar to its mammalian counterpart, Drosophila RNase Z(L) (dRNaseZ) has a mitochondria targeting signal (MTS) flanked by two methionines at the N-terminus...
  6. Dubrovsky E, Dubrovskaya V, Bilderback A, Berger E. The isolation of two juvenile hormone-inducible genes in Drosophila melanogaster. Dev Biol. 2000;224:486-95 pubmed
    ..The developmental expression of the two JH-inducible genes corresponds to the abundance profile of JH in vivo. Furthermore, topical methoprene application to pupae leads to the ectopic accumulation of JhI-1 and JhI-26 transcripts. ..
  7. Xie X, Dubrovskaya V, Yacoub N, Walska J, Gleason T, Reid K, et al. Developmental roles of Drosophila tRNA processing endonuclease RNase ZL as revealed with a conditional rescue system. Dev Biol. 2013;381:324-40 pubmed publisher
    Drosophila RNase Z(L) (dRNaseZ) belongs to a family of endoribonucleases with a major role in tRNA 3'-end processing. The biochemical function of RNase Z(L) is conserved from yeast to human...
  8. Saoura M, Pinnock K, Pujantell Graell M, Levinger L. Substitutions in conserved regions preceding and within the linker affect activity and flexibility of tRNase ZL, the long form of tRNase Z. PLoS ONE. 2017;12:e0186277 pubmed publisher
    The enzyme tRNase Z, a member of the metallo-?-lactamase family, endonucleolytically removes 3' trailers from precursor tRNAs, preparing them for CCA addition and aminoacylation...
  9. Dubrovsky E, Dubrovskaya V, Levinger L, Schiffer S, Marchfelder A. Drosophila RNase Z processes mitochondrial and nuclear pre-tRNA 3' ends in vivo. Nucleic Acids Res. 2004;32:255-62 pubmed
    ..In addition our data show that in vivo 5' processing of nuclear and mitochondrial pre-tRNAs occurs before 3' processing. ..

More Information


  1. Xie X, Dubrovskaya V, Dubrovsky E. RNAi knockdown of dRNaseZ, the Drosophila homolog of ELAC2, impairs growth of mitotic and endoreplicating tissues. Insect Biochem Mol Biol. 2011;41:167-77 pubmed publisher
    The Drosophila RNase Z(L) (dRNaseZ) gene encodes a member of the ELAC1/ELAC2 protein family with homologs in every living organism...
  2. Karkashon S, Hopkinson A, Levinger L. tRNase Z catalysis and conserved residues on the carboxy side of the His cluster. Biochemistry. 2007;46:9380-7 pubmed
    ..The 3'-end trailer can be removed by the pre-tRNA processing endonuclease tRNase Z, an ancient, conserved member of the beta-lactamase superfamily of metal-dependent hydrolases...