Iswi

Summary

Gene Symbol: Iswi
Description: Imitation SWI
Alias: ACF, CG8625, CHRAC, Dmel\CG8625, ISW, ISWI, NURF, NURF 140, NURF-140, Nurf-140, SWI, anon-EP1279744.124, dCHRAC, dISWI, dNURF, iswi, p140, p140/ISWI, imitation SWI, CG8625-PA, CG8625-PB, CG8625-PC, ISWI, ISWI ATPase, Iswi-PA, Iswi-PB, Iswi-PC, imitation SW, imitation switch, imitation-SWI, nucleosome remodeling factor, nucleosome remodeling factor - 140kD
Species: fruit fly
Products:     Iswi

Top Publications

  1. Arancio W, Onorati M, Burgio G, Collesano M, Ingrassia A, Genovese S, et al. The nucleosome remodeling factor ISWI functionally interacts with an evolutionarily conserved network of cellular factors. Genetics. 2010;185:129-40 pubmed publisher
    b>ISWI is an evolutionarily conserved ATP-dependent chromatin remodeling factor playing central roles in DNA replication, RNA transcription, and chromosome organization...
  2. Carré C, Ciurciu A, Komonyi O, Jacquier C, Fagegaltier D, Pidoux J, et al. The Drosophila NURF remodelling and the ATAC histone acetylase complexes functionally interact and are required for global chromosome organization. EMBO Rep. 2008;9:187-92 pubmed
    ..of the male X chromosome, similar to that induced by mutations in the Iswi and Nurf301 subunits of the NURF nucleosome remodelling complex...
  3. Whitehouse I, Stockdale C, Flaus A, Szczelkun M, Owen Hughes T. Evidence for DNA translocation by the ISWI chromatin-remodeling enzyme. Mol Cell Biol. 2003;23:1935-45 pubmed
    The ISWI proteins form the catalytic core of a subset of ATP-dependent chromatin-remodeling activities. Here, we studied the interaction of the ISWI protein with nucleosomal substrates...
  4. Xi R, Xie T. Stem cell self-renewal controlled by chromatin remodeling factors. Science. 2005;310:1487-9 pubmed
    ..Here we report that the adenosine triphosphate-dependent chromatin remodeling factors ISWI and DOM control germline stem cell and somatic stem cell self-renewal in the Drosophila ovary, respectively...
  5. Mueller Planitz F, Klinker H, Ludwigsen J, Becker P. The ATPase domain of ISWI is an autonomous nucleosome remodeling machine. Nat Struct Mol Biol. 2013;20:82-9 pubmed publisher
    ..Prominent mechanistic models imply cooperation of the ISWI ATPase domain with a C-terminal DNA-binding function residing in the HAND-SANT-SLIDE (HSS) domain...
  6. Corona D, Tamkun J. Multiple roles for ISWI in transcription, chromosome organization and DNA replication. Biochim Biophys Acta. 2004;1677:113-9 pubmed
    b>ISWI functions as the ATPase subunit of multiple chromatin-remodeling complexes...
  7. Vanolst L, Fromental Ramain C, Ramain P. Toutatis, a TIP5-related protein, positively regulates Pannier function during Drosophila neural development. Development. 2005;132:4327-38 pubmed
    ..In contrast, Tou acts positively to activate proneural gene expression. Moreover, we show that Iswi associates with Tou, Pnr and Chip, and is also required during Pnr-driven neural development...
  8. Corona D, Eberharter A, Budde A, Deuring R, Ferrari S, Varga Weisz P, et al. Two histone fold proteins, CHRAC-14 and CHRAC-16, are developmentally regulated subunits of chromatin accessibility complex (CHRAC). EMBO J. 2000;19:3049-59 pubmed
    The ISWI ATPase of Drosophila is a molecular engine that can drive a range of nucleosome remodelling reactions in vitro. ISWI is important for cell viability, developmental gene expression and chromosome structure...
  9. Burgio G, La Rocca G, Sala A, Arancio W, Di Gesù D, Collesano M, et al. Genetic identification of a network of factors that functionally interact with the nucleosome remodeling ATPase ISWI. PLoS Genet. 2008;4:e1000089 pubmed publisher
    ..The evolutionarily conserved ATP-dependent chromatin-remodeling factor ISWI plays essential roles in chromosome organization, DNA replication, and transcription regulation...

More Information

Publications65

  1. Ito T, Bulger M, Pazin M, Kobayashi R, Kadonaga J. ACF, an ISWI-containing and ATP-utilizing chromatin assembly and remodeling factor. Cell. 1997;90:145-55 pubmed
    ..ACF is a multisubunit factor that contains ISWI protein and is distinct from NURF, another ISWI-containing factor...
  2. Cherry C, MATUNIS E. Epigenetic regulation of stem cell maintenance in the Drosophila testis via the nucleosome-remodeling factor NURF. Cell Stem Cell. 2010;6:557-67 pubmed publisher
    ..Because three additional ATP-dependent chromatin remodelers (ACF, CHRAC, and dMi-2/NuRD) are dispensable for stem cell maintenance in the testis, epigenetic regulation of stem cells ..
  3. Song H, Spichiger Haeusermann C, Basler K. The ISWI-containing NURF complex regulates the output of the canonical Wingless pathway. EMBO Rep. 2009;10:1140-6 pubmed publisher
    ..In a genetic screen for Wg signalling components, we found that imitation switch (ISWI), a chromatin-remodelling ATPase, had a positive role in transducing the canonical Wg signal, promoting ..
  4. Corona D, Langst G, Clapier C, Bonte E, Ferrari S, Tamkun J, et al. ISWI is an ATP-dependent nucleosome remodeling factor. Mol Cell. 1999;3:239-45 pubmed
    The ATPase ISWI is a subunit of several distinct nucleosome remodeling complexes that increase the accessibility of DNA in chromatin...
  5. Deuring R, Fanti L, Armstrong J, Sarte M, Papoulas O, Prestel M, et al. The ISWI chromatin-remodeling protein is required for gene expression and the maintenance of higher order chromatin structure in vivo. Mol Cell. 2000;5:355-65 pubmed
    ..These findings reveal novel functions for the ISWI ATPase and underscore its importance in chromatin remodeling in vivo.
  6. Sala A, Toto M, Pinello L, Gabriele A, Di Benedetto V, Ingrassia A, et al. Genome-wide characterization of chromatin binding and nucleosome spacing activity of the nucleosome remodelling ATPase ISWI. EMBO J. 2011;30:1766-77 pubmed publisher
    The evolutionarily conserved ATP-dependent nucleosome remodelling factor ISWI can space nucleosomes affecting a variety of nuclear processes...
  7. Fry C, Peterson C. Chromatin remodeling enzymes: who's on first?. Curr Biol. 2001;11:R185-97 pubmed
    ..that activators must first target two types of chromatin remodeling enzyme to the promoter region: an ATP-dependent SWI/SNF-like complex and a histone acetyltransferase...
  8. Fyodorov D, Blower M, Karpen G, Kadonaga J. Acf1 confers unique activities to ACF/CHRAC and promotes the formation rather than disruption of chromatin in vivo. Genes Dev. 2004;18:170-83 pubmed
    ..catalyzes the ATP-dependent assembly of periodic nucleosome arrays in vitro, and consists of Acf1 and the ISWI ATPase. Acf1 and ISWI are also subunits of CHRAC (chromatin accessibility complex), whose biochemical activities are ..
  9. Armstrong J, Papoulas O, Daubresse G, Sperling A, Lis J, Scott M, et al. The Drosophila BRM complex facilitates global transcription by RNA polymerase II. EMBO J. 2002;21:5245-54 pubmed
    Drosophila brahma (brm) encodes the ATPase subunit of a 2 MDa complex that is related to yeast SWI/SNF and other chromatin-remodeling complexes...
  10. Kirilly D, Wong J, Lim E, Wang Y, Zhang H, Wang C, et al. Intrinsic epigenetic factors cooperate with the steroid hormone ecdysone to govern dendrite pruning in Drosophila. Neuron. 2011;72:86-100 pubmed publisher
    ..Thus, specific intrinsic epigenetic factors cooperate with steroid hormones to activate selective transcriptional programs, thereby initiating neuronal remodeling. ..
  11. Corona D, Siriaco G, Armstrong J, Snarskaya N, McClymont S, Scott M, et al. ISWI regulates higher-order chromatin structure and histone H1 assembly in vivo. PLoS Biol. 2007;5:e232 pubmed
    b>Imitation SWI (ISWI) and other ATP-dependent chromatin-remodeling factors play key roles in transcription and other processes by altering the structure and positioning of nucleosomes...
  12. Varga Weisz P, Becker P. Regulation of higher-order chromatin structures by nucleosome-remodelling factors. Curr Opin Genet Dev. 2006;16:151-6 pubmed
    ..Recent observations support roles for nucleosome-remodelling factors at the supra-nucleosomal level. ..
  13. Martinez Balbas M, Tsukiyama T, Gdula D, Wu C. Drosophila NURF-55, a WD repeat protein involved in histone metabolism. Proc Natl Acad Sci U S A. 1998;95:132-7 pubmed
    The Drosophila nucleosome remodeling factor (NURF) is a protein complex of four distinct subunits that assists transcription factor-mediated chromatin remodeling...
  14. Kal A, Mahmoudi T, Zak N, Verrijzer C. The Drosophila brahma complex is an essential coactivator for the trithorax group protein zeste. Genes Dev. 2000;14:1058-71 pubmed
    ..The leucine zipper of Zeste mediates binding to MOR. Interestingly, although the Imitation Switch (ISWI) remodelers are potent nucleosome spacing factors, they are dispensable for transcriptional activation ..
  15. Corona D, Clapier C, Becker P, Tamkun J. Modulation of ISWI function by site-specific histone acetylation. EMBO Rep. 2002;3:242-7 pubmed
    ..These findings suggest that H4K16 acetylation directly counteracts chromatin compaction mediated by the ISWI ATPase.
  16. Khattak S, Lee B, Cho S, Ahnn J, Spoerel N. Genetic characterization of Drosophila Mi-2 ATPase. Gene. 2002;293:107-14 pubmed
    ..The expression pattern of dMi-2 and the embryonic lethal phenotypes of mutants indicate that dMi-2 is essential for embryonic development in Drosophila melanagaster. ..
  17. Collins R, Furukawa T, Tanese N, Treisman J. Osa associates with the Brahma chromatin remodeling complex and promotes the activation of some target genes. EMBO J. 1999;18:7029-40 pubmed
    The yeast SWI/SNF complex and its Drosophila and mammalian homologs are thought to control gene expression by altering chromatin structure, but the mechanism and specificity of this process are not fully understood...
  18. Bonaldi T, Längst G, Strohner R, Becker P, Bianchi M. The DNA chaperone HMGB1 facilitates ACF/CHRAC-dependent nucleosome sliding. EMBO J. 2002;21:6865-73 pubmed
    Nucleosome remodelling complexes CHRAC and ACF contribute to chromatin dynamics by converting chemical energy into sliding of histone octamers on DNA. Their shared ATPase subunit ISWI binds DNA at the sites of entry into the nucleosome...
  19. Eberharter A, Vetter I, Ferreira R, Becker P. ACF1 improves the effectiveness of nucleosome mobilization by ISWI through PHD-histone contacts. EMBO J. 2004;23:4029-39 pubmed
    ..Association of ISWI with ACF1, the largest subunit of CHRAC and ACF complexes, improves the efficiency of ISWI-induced nucleosome mobilization by an order of magnitude and ..
  20. Li M, Belozerov V, Cai H. Modulation of chromatin boundary activities by nucleosome-remodeling activities in Drosophila melanogaster. Mol Cell Biol. 2010;30:1067-76 pubmed publisher
    ..We found that knockdown of NURF301 and ISWI, key components of the nucleosome-remodeling factor (NURF), synergistically disrupted the enhancer-blocking function of Fab7 and SF1 and augmented the function of Fab8...
  21. Tsukiyama T, Daniel C, Tamkun J, Wu C. ISWI, a member of the SWI2/SNF2 ATPase family, encodes the 140 kDa subunit of the nucleosome remodeling factor. Cell. 1995;83:1021-6 pubmed
    ..in vitro requires the concerted action of the GAGA transcription factor and NURF, an ATP-dependent nucleosome remodeling factor. NURF is composed of four subunits and is biochemically distinct from the SWI2/SNF2 multiprotein ..
  22. Siriaco G, Deuring R, Chioda M, Becker P, Tamkun J. Drosophila ISWI regulates the association of histone H1 with interphase chromosomes in vivo. Genetics. 2009;182:661-9 pubmed publisher
    ..Recent studies suggest that the ISWI chromatin-remodeling factor plays a key role in this process by promoting the assembly of chromatin containing ..
  23. Tsukiyama T, Wu C. Purification and properties of an ATP-dependent nucleosome remodeling factor. Cell. 1995;83:1011-20 pubmed
    We report the purification of an ATP-dependent nucleosome remodeling factor (NURF) from Drosophila embryo extracts...
  24. Langst G, Becker P. ISWI induces nucleosome sliding on nicked DNA. Mol Cell. 2001;8:1085-92 pubmed
    The ATPase ISWI is the molecular motor of several remodeling factors that trigger nucleosome sliding in vitro...
  25. Eberharter A, Ferrari S, Langst G, Straub T, Imhof A, Varga Weisz P, et al. Acf1, the largest subunit of CHRAC, regulates ISWI-induced nucleosome remodelling. EMBO J. 2001;20:3781-8 pubmed
    The chromatin accessibility complex (CHRAC) was originally defined biochemically as an ATP-dependent 'nucleosome remodelling' activity...
  26. Georgel P, Tsukiyama T, Wu C. Role of histone tails in nucleosome remodeling by Drosophila NURF. EMBO J. 1997;16:4717-26 pubmed
    The Drosophila nucleosome remodeling factor NURF utilizes the energy of ATP hydrolysis to perturb the structure of nucleosomes and facilitate binding of transcription factors...
  27. Reddy B, Bajpe P, Bassett A, Moshkin Y, Kozhevnikova E, Bezstarosti K, et al. Drosophila transcription factor Tramtrack69 binds MEP1 to recruit the chromatin remodeler NuRD. Mol Cell Biol. 2010;30:5234-44 pubmed publisher
    ..Thus, there appears to be a hierarchical relationship in which transcription factor binding precedes remodeler recruitment. ..
  28. Clapier C, Nightingale K, Becker P. A critical epitope for substrate recognition by the nucleosome remodeling ATPase ISWI. Nucleic Acids Res. 2002;30:649-55 pubmed
    ..Consistent with this hypothesis, the ISWI ATPase is stimulated by isolated H4 tail peptides ISWI only in the presence of DNA...
  29. Brehm A, Langst G, Kehle J, Clapier C, Imhof A, Eberharter A, et al. dMi-2 and ISWI chromatin remodelling factors have distinct nucleosome binding and mobilization properties. EMBO J. 2000;19:4332-41 pubmed
    Mi-2 and ISWI, two members of the Snf2 superfamily of ATPases, reside in separate ATP-dependent chromatin remodelling complexes...
  30. Ferreira R, Eberharter A, Bonaldi T, Chioda M, Imhof A, Becker P. Site-specific acetylation of ISWI by GCN5. BMC Mol Biol. 2007;8:73 pubmed
    ..The histone acetyltransferase GCN5 can acetylate the Drosophila remodelling ATPase ISWI at a single, conserved lysine, K753, in vivo and in vitro...
  31. Schwanbeck R, Xiao H, Wu C. Spatial contacts and nucleosome step movements induced by the NURF chromatin remodeling complex. J Biol Chem. 2004;279:39933-41 pubmed
    The nucleosome remodeling factor NURF is a four-subunit, ISWI-containing chromatin remodeling complex that catalyzes nucleosome sliding in an ATP-dependent fashion, thereby modulating the accessibility of the DNA...
  32. Okada M, Hirose S. Chromatin remodeling mediated by Drosophila GAGA factor and ISWI activates fushi tarazu gene transcription in vitro. Mol Cell Biol. 1998;18:2455-61 pubmed
    GAGA factor is known to remodel the chromatin structure in concert with nucleosome-remodeling factor NURF in a Drosophila embryonic S150 extract...
  33. Ito T, Levenstein M, Fyodorov D, Kutach A, Kobayashi R, Kadonaga J. ACF consists of two subunits, Acf1 and ISWI, that function cooperatively in the ATP-dependent catalysis of chromatin assembly. Genes Dev. 1999;13:1529-39 pubmed
    ..Native Acf1 did not copurify with components of NURF or CHRAC, which are other ISWI-containing complexes in Drosophila...
  34. Hamiche A, Sandaltzopoulos R, Gdula D, Wu C. ATP-dependent histone octamer sliding mediated by the chromatin remodeling complex NURF. Cell. 1999;97:833-42 pubmed
    Drosophila NURF is an ATP-dependent chromatin remodeling complex that contains ISWI, a member of the SWI2/SNF2 family of ATPases...
  35. Petesch S, Lis J. Rapid, transcription-independent loss of nucleosomes over a large chromatin domain at Hsp70 loci. Cell. 2008;134:74-84 pubmed publisher
    ..An RNAi screen of 28 transcription and chromatin-related factors reveals that depletion of heat shock factor, GAGA Factor, or Poly(ADP)-Ribose Polymerase or its activity abolishes the loss of nucleosomes upon Hsp70 activation. ..
  36. Grüne T, Brzeski J, Eberharter A, Clapier C, Corona D, Becker P, et al. Crystal structure and functional analysis of a nucleosome recognition module of the remodeling factor ISWI. Mol Cell. 2003;12:449-60 pubmed
    ..We have identified a substrate recognition domain in the C-terminal half of the remodeling ATPase ISWI and determined its structure by X-ray crystallography...
  37. Wysocka J, Swigut T, Xiao H, Milne T, Kwon S, Landry J, et al. A PHD finger of NURF couples histone H3 lysine 4 trimethylation with chromatin remodelling. Nature. 2006;442:86-90 pubmed
    ..Here we show that a plant homeodomain (PHD) finger of nucleosome remodelling factor (NURF), an ISWI-containing ATP-dependent chromatin-remodelling complex, mediates a direct preferential association with H3K4me3 ..
  38. Langst G, Bonte E, Corona D, Becker P. Nucleosome movement by CHRAC and ISWI without disruption or trans-displacement of the histone octamer. Cell. 1999;97:843-52 pubmed
    The chromatin accessibility complex (CHRAC) belongs to the class of nucleosome remodeling factors that increase the accessibility of nucleosomal DNA in an ATP-dependent manner...
  39. Shogren Knaak M, Ishii H, Sun J, Pazin M, Davie J, Peterson C. Histone H4-K16 acetylation controls chromatin structure and protein interactions. Science. 2006;311:844-7 pubmed
    ..H4-K16Ac also inhibits the ability of the adenosine triphosphate-utilizing chromatin assembly and remodeling enzyme ACF to mobilize a mononucleosome, indicating that this single histone modification modulates both higher order ..
  40. Srinivasan S, Armstrong J, Deuring R, Dahlsveen I, McNeill H, Tamkun J. The Drosophila trithorax group protein Kismet facilitates an early step in transcriptional elongation by RNA Polymerase II. Development. 2005;132:1623-35 pubmed
    ..By contrast, the loss of KIS-L function does not affect the binding of PC to chromatin or the recruitment of Pol II to promoters. These data suggest that KIS-L facilitates an early step in transcriptional elongation by Pol II. ..
  41. Bai X, Larschan E, Kwon S, Badenhorst P, Kuroda M. Regional control of chromatin organization by noncoding roX RNAs and the NURF remodeling complex in Drosophila melanogaster. Genetics. 2007;176:1491-9 pubmed
    ..Here we use a sensitized system to examine the function of roX genes on the X chromosome. In mutants that lack the NURF nucleosome remodeling complex, the male polytene X chromosome is severely distorted, appearing decondensed...
  42. Gdula D, Sandaltzopoulos R, Tsukiyama T, Ossipow V, Wu C. Inorganic pyrophosphatase is a component of the Drosophila nucleosome remodeling factor complex. Genes Dev. 1998;12:3206-16 pubmed
    The Drosophila nucleosome remodeling factor (NURF) is a protein complex consisting of four polypeptides that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner...
  43. Bhadra M, Bhadra U, Kundu J, Birchler J. Gene expression analysis of the function of the male-specific lethal complex in Drosophila. Genetics. 2005;169:2061-74 pubmed
    ..Mutations in the ISWI chromatin-remodeling component cause increases specifically of X-linked genes in males...
  44. Clapier C, Cairns B. Regulation of ISWI involves inhibitory modules antagonized by nucleosomal epitopes. Nature. 2012;492:280-4 pubmed publisher
    ..Thus, the ISWI ATPase catalytic core is an intrinsically active DNA translocase that conducts nucleosome sliding, onto which ..
  45. Moshkin Y, Chalkley G, Kan T, Reddy B, Ozgur Z, van Ijcken W, et al. Remodelers organize cellular chromatin by counteracting intrinsic histone-DNA sequence preferences in a class-specific manner. Mol Cell Biol. 2012;32:675-88 pubmed publisher
    ..We compared the genome-wide distribution of Drosophila NURD, (P)BAP, INO80, and ISWI, representing the four major remodeler families...
  46. Kugler S, Nagel A. A novel Pzg-NURF complex regulates Notch target gene activity. Mol Biol Cell. 2010;21:3443-8 pubmed publisher
    ..Here, we show that Putzig associates with the NURF complex...
  47. LeRoy G, Orphanides G, Lane W, Reinberg D. Requirement of RSF and FACT for transcription of chromatin templates in vitro. Science. 1998;282:1900-4 pubmed
    ..Thus, the minimal factor requirements for activator-dependent transcription on chromatin templates in vitro have been defined. ..
  48. Nakayama T, Shimojima T, Hirose S. The PBAP remodeling complex is required for histone H3.3 replacement at chromatin boundaries and for boundary functions. Development. 2012;139:4582-90 pubmed publisher
    ..3 replacement to execute boundary functions. Our results provide new insight into the function of the trithorax group during development. ..
  49. Varga Weisz P, Wilm M, Bonte E, Dumas K, Mann M, Becker P. Chromatin-remodelling factor CHRAC contains the ATPases ISWI and topoisomerase II. Nature. 1997;388:598-602 pubmed
    ..We have now identified a chromatin-accessibility complex (CHRAC) which uses energy to increase the general accessibility of DNA in chromatin...
  50. Badenhorst P, Voas M, Rebay I, Wu C. Biological functions of the ISWI chromatin remodeling complex NURF. Genes Dev. 2002;16:3186-98 pubmed
    The nucleosome remodeling factor (NURF) is one of several ISWI-containing protein complexes that catalyze ATP-dependent nucleosome sliding and facilitate transcription of chromatin in vitro...
  51. Strohner R, Wachsmuth M, Dachauer K, Mazurkiewicz J, Hochstatter J, Rippe K, et al. A 'loop recapture' mechanism for ACF-dependent nucleosome remodeling. Nat Struct Mol Biol. 2005;12:683-90 pubmed
    The ATPase ISWI is the molecular motor of several nucleosome remodeling complexes including ACF. We analyzed the ACF-nucleosome interactions and determined the characteristics of ACF-dependent nucleosome remodeling...
  52. Xiao H, Sandaltzopoulos R, Wang H, Hamiche A, Ranallo R, Lee K, et al. Dual functions of largest NURF subunit NURF301 in nucleosome sliding and transcription factor interactions. Mol Cell. 2001;8:531-43 pubmed
    ..We have reconstituted full and partial NURF complexes from recombinant proteins and show that NURF301 and the ISWI ATPase are necessary and sufficient for accurate and efficient nucleosome sliding...
  53. Lusser A, Urwin D, Kadonaga J. Distinct activities of CHD1 and ACF in ATP-dependent chromatin assembly. Nat Struct Mol Biol. 2005;12:160-6 pubmed
    ..The comparative analysis of CHD1 and ACF revealed that CHD1 assembles chromatin with a shorter nucleosome repeat length than ACF...
  54. Henikoff S, Henikoff J, Sakai A, Loeb G, Ahmad K. Genome-wide profiling of salt fractions maps physical properties of chromatin. Genome Res. 2009;19:460-9 pubmed publisher
  55. Chalkley G, Moshkin Y, Langenberg K, Bezstarosti K, Blastyak A, Gyurkovics H, et al. The transcriptional coactivator SAYP is a trithorax group signature subunit of the PBAP chromatin remodeling complex. Mol Cell Biol. 2008;28:2920-9 pubmed publisher
    b>SWI/SNF ATP-dependent chromatin remodeling complexes (remodelers) perform critical functions in eukaryotic gene expression control...
  56. Straub T, Becker P. Dosage compensation: the beginning and end of generalization. Nat Rev Genet. 2007;8:47-57 pubmed