Gene Symbol: ImpL3
Description: Ecdysone-inducible gene L3
Alias: CG10160, Dmel\CG10160, IMP-L3, IMPL3, Imp-L3, Impl 3, Impl3, LDH, Ldh, dLDH, dLdha, dmLDH, impl3, ldh, lincRNA.S4223, Ecdysone-inducible gene L3, CG10160-PA, CG10160-PB, CG10160-PC, CG10160-PD, Ecdysone-induced gene L3, ImpL3-PA, ImpL3-PB, ImpL3-PC, ImpL3-PD, lactate dehydrogenase, lactate dehydrogenase B, lactate/malate dehydrogenease, lactic DH
Species: fruit fly

Top Publications

  1. Dekanty A, Lavista Llanos S, Irisarri M, Oldham S, Wappner P. The insulin-PI3K/TOR pathway induces a HIF-dependent transcriptional response in Drosophila by promoting nuclear localization of HIF-alpha/Sima. J Cell Sci. 2005;118:5431-41 pubmed
    ..We conclude that regulation of HIF-alpha/Sima by the PI3K-AKT-TOR pathway is a major conserved mode of regulation of the HIF-dependent transcriptional response in Drosophila. ..
  2. van Uden P, Kenneth N, Webster R, Müller H, Mudie S, Rocha S. Evolutionary conserved regulation of HIF-1β by NF-κB. PLoS Genet. 2011;7:e1001285 pubmed publisher
    ..Significantly, NF-κB regulates HIF-1β (tango) and HIF-α (sima) levels and activity (Hph/fatiga, ImpL3/ldha) in Drosophila, both in normoxia and hypoxia, indicating an evolutionary conserved mode of regulation...
  3. Bruick R, McKnight S. A conserved family of prolyl-4-hydroxylases that modify HIF. Science. 2001;294:1337-40 pubmed
    ..cells by RNA interference resulted in elevated expression of a hypoxia-inducible gene (LDH, encoding lactate dehydrogenase) under normoxic conditions...
  4. Tennessen J, Baker K, Lam G, Evans J, Thummel C. The Drosophila estrogen-related receptor directs a metabolic switch that supports developmental growth. Cell Metab. 2011;13:139-48 pubmed publisher
  5. Arking R, Burde V, Graves K, Hari R, Feldman E, Zeevi A, et al. Forward and reverse selection for longevity in Drosophila is characterized by alteration of antioxidant gene expression and oxidative damage patterns. Exp Gerontol. 2000;35:167-85 pubmed
  6. Gorr T, Tomita T, Wappner P, Bunn H. Regulation of Drosophila hypoxia-inducible factor (HIF) activity in SL2 cells: identification of a hypoxia-induced variant isoform of the HIFalpha homolog gene similar. J Biol Chem. 2004;279:36048-58 pubmed
    ..Tango)-stimulated reporter expression in a dose-dependent manner. Thus, svSima has the potential to regulate Drosophila HIF function under steady and hypoxic pO(2) by creating a cytosolic sink for the Sima partner protein Tango. ..
  7. Li Y, Padmanabha D, Gentile L, Dumur C, Beckstead R, Baker K. HIF- and non-HIF-regulated hypoxic responses require the estrogen-related receptor in Drosophila melanogaster. PLoS Genet. 2013;9:e1003230 pubmed publisher
    ..These results indicate that competent hypoxic responses arise from complex interactions between HIF-dependent and -independent mechanisms, and that dERR plays a central role in both of these programs. ..
  8. Mortimer N, Moberg K. The archipelago ubiquitin ligase subunit acts in target tissue to restrict tracheal terminal cell branching and hypoxic-induced gene expression. PLoS Genet. 2013;9:e1003314 pubmed publisher
    ..Collectively, these findings identify Ago as a required element of a circuit that suppresses the tracheogenic activity of larval muscle cells by antagonizing the Sima-mediated transcriptional response to hypoxia. ..
  9. Lee M, Toh L, Yaw L, Luo Y. Drosophila octamer elements and Pdm-1 dictate the coordinated transcription of core histone genes. J Biol Chem. 2010;285:9041-53 pubmed publisher
    ..Finally, dmOCA-S is recruited to the Drosophila histone locus bodies in the S-phase, marking S-phase-specific transcription activation of core histone genes. ..

More Information


  1. Tixier V, Bataillé L, Etard C, Jagla T, Weger M, Daponte J, et al. Glycolysis supports embryonic muscle growth by promoting myoblast fusion. Proc Natl Acad Sci U S A. 2013;110:18982-7 pubmed publisher
    ..screen identifies six other genes involved in glycolysis or in pyruvate metabolism (Pfk, Tpi, Gapdh, Pgk, Pyk, and Impl3)...
  2. Kamps Hughes N, Preston J, Randel M, Johnson E. Genome-wide identification of hypoxia-induced enhancer regions. Peerj. 2015;3:e1527 pubmed publisher
    ..This work provides a novel method for high-throughput assay of enhancer activity and the genome-scale identification of 31 hypoxia-activated enhancers in Drosophila. ..
  3. Park S, Ludwig M, Tamarina N, He B, Carl S, Dickerson D, et al. Genetic complexity in a Drosophila model of diabetes-associated misfolded human proinsulin. Genetics. 2014;196:539-55 pubmed publisher
    ..The genetic dominance of disease-specific phenotypic variability in our model of misfolded human proinsulin makes this approach amenable to genome-wide association study in a simple F1 screen of natural variation. ..
  4. Cagin U, Duncan O, Gatt A, Dionne M, Sweeney S, Bateman J. Mitochondrial retrograde signaling regulates neuronal function. Proc Natl Acad Sci U S A. 2015;112:E6000-9 pubmed publisher
    ..Thus, mitochondrial retrograde signaling regulates neuronal activity and can be manipulated to enhance neuronal function, despite mitochondrial impairment. ..
  5. Xie X, Dubrovsky E. Knockout of Drosophila RNase ZL impairs mitochondrial transcript processing, respiration and cell cycle progression. Nucleic Acids Res. 2015;43:10364-75 pubmed publisher
    ..This study implicates Drosophila RNase Z(L) in a novel retrograde signaling pathway initiated by the damage in mitochondria and manifested in a cell cycle delay before the mitotic entry. ..
  6. Slaninova V, Krafcikova M, Perez Gomez R, Steffal P, Trantirek L, Bray S, et al. Notch stimulates growth by direct regulation of genes involved in the control of glycolysis and the tricarboxylic acid cycle. Open Biol. 2016;6:150155 pubmed publisher
    ..Notch is active in situations that involve metabolic reprogramming, and the direct regulation of metabolic genes may be a common mechanism that helps Notch to exert its effects in target tissues. ..
  7. Scialò F, Sriram A, Fernández Ayala D, Gubina N, Lõhmus M, Nelson G, et al. Mitochondrial ROS Produced via Reverse Electron Transport Extend Animal Lifespan. Cell Metab. 2016;23:725-34 pubmed publisher
    ..These results illustrate that the source of a ROS signal is vital in determining its effects on cellular physiology and establish that manipulation of ubiquinone redox state is a valid strategy to delay aging. ..
  8. van Bergeijk P, Heimiller J, Uyetake L, Su T. Genome-wide expression analysis identifies a modulator of ionizing radiation-induced p53-independent apoptosis in Drosophila melanogaster. PLoS ONE. 2012;7:e36539 pubmed publisher
    ..These and other results support the emerging notion that there is not a single dominant mechanism but that both positive and negative inputs collaborate to induce p53-independent apoptosis in response to IR in Drosophila larvae. ..
  9. Kunert N, Marhold J, Stanke J, Stach D, Lyko F. A Dnmt2-like protein mediates DNA methylation in Drosophila. Development. 2003;130:5083-90 pubmed
    ..These results demonstrate that Dnmt2 is both necessary and sufficient for DNA methylation in Drosophila and suggest a novel CpT/A-specific DNA methyltransferase activity for Dnmt2 proteins. ..
  10. Abu Shumays R, Fristrom J. IMP-L3, A 20-hydroxyecdysone-responsive gene encodes Drosophila lactate dehydrogenase: structural characterization and developmental studies. Dev Genet. 1997;20:11-22 pubmed
    ..a gene isolated as a potential mediator of imaginal disc morphogenesis in Drosophila melanogaster, encodes lactate dehydrogenase (LDH). The predicted amino acid sequence of IMP-L3 is 58-61% identical to those of human LDHs...
  11. Ciurciu A, Duncalf L, Jonchère V, Lansdale N, Vasieva O, Glenday P, et al. PNUTS/PP1 regulates RNAPII-mediated gene expression and is necessary for developmental growth. PLoS Genet. 2013;9:e1003885 pubmed publisher
    ..Together, these findings shed light on the in vivo role of the PNUTS-PP1 holoenzyme and its contribution to the control of gene expression during early Drosophila development...
  12. Bandarra D, Biddlestone J, Mudie S, Müller H, Rocha S. HIF-1α restricts NF-κB-dependent gene expression to control innate immunity signals. Dis Model Mech. 2015;8:169-81 pubmed publisher
    ..These results indicate that HIF-1α is required to restrain the NF-κB response, and thus prevents excessive and damaging pro-inflammatory responses. ..
  13. Krebs R, Holbrook S. Reduced enzyme activity following Hsp70 overexpression in Drosophila melanogaster. Biochem Genet. 2001;39:73-82 pubmed
    ..activity of both enzymes analyzed, adult alcohol dehydrogenase (ADH), which is heat sensitive, and lactate dehydrogenase, which is not...
  14. van der Knaap J, Kozhevnikova E, Langenberg K, Moshkin Y, Verrijzer C. Biosynthetic enzyme GMP synthetase cooperates with ubiquitin-specific protease 7 in transcriptional regulation of ecdysteroid target genes. Mol Cell Biol. 2010;30:736-44 pubmed publisher
    ..These results revealed the cooperation between a biosynthetic enzyme and a ubiquitin protease in developmental gene control by hormone receptors. ..
  15. de la Cova C, Senoo Matsuda N, Ziosi M, Wu D, Bellosta P, Quinzii C, et al. Supercompetitor status of Drosophila Myc cells requires p53 as a fitness sensor to reprogram metabolism and promote viability. Cell Metab. 2014;19:470-83 pubmed publisher
    ..Our findings suggest that the initial confrontation between precancerous and WT cells could enhance cancer cell fitness and promote tumor progression. ..
  16. Arking R, Burde V, Graves K, Hari R, Feldman E, Zeevi A, et al. Identical longevity phenotypes are characterized by different patterns of gene expression and oxidative damage. Exp Gerontol. 2000;35:353-73 pubmed
    ..The theoretical and empirical implications of these findings are discussed. ..
  17. Natzle J, Hammonds A, Fristrom J. Isolation of genes active during hormone-induced morphogenesis in Drosophila imaginal discs. J Biol Chem. 1986;261:5575-83 pubmed
    ..These genes constitute a new set of 20-hydroxyecdysone responsive loci that may encode gene products specifically required for imaginal disc morphogenesis. ..
  18. Leandro L, Szewczyk N, Benguria A, Herranz R, LaVan D, Medina F, et al. Comparative analysis of Drosophila melanogaster and Caenorhabditis elegans gene expression experiments in the European Soyuz flights to the International Space Station. Adv Space Res. 2007;40:506-512 pubmed
  19. Karvountzi E, Kilias G, Alahiotis S. Drosophila lactate dehydrogenase. Functional and evolutionary aspects. Hereditas. 1995;123:61-7 pubmed
    The functional characteristics of homogeneously purified LDH were studied in the eight D. melanogaster species subgroup at two different growing temperatures (14 degrees C, 25 degrees C). The Vmax, kcat, Vmax/KeNAD...
  20. Ritter A, Beckstead R. Sox14 is required for transcriptional and developmental responses to 20-hydroxyecdysone at the onset of drosophila metamorphosis. Dev Dyn. 2010;239:2685-94 pubmed publisher
    ..We also show that the Sox14-regulated gene set correlates well with Sox14 expression in a variety of larval and adult tissues. Thus, Sox14 is a critical transcription factor required for 20E signaling at the onset of metamorphosis. ..
  21. Sansone C, Blumenthal E. Neurodegeneration in drop-dead mutant drosophila melanogaster is associated with the respiratory system but not with Hypoxia. PLoS ONE. 2013;8:e68032 pubmed publisher
    ..Hypoxia-sensitive reporter transgenes (LDH-Gal4 and LDH-LacZ) were placed on a drd mutant background, but enhanced expression of these reporters was not ..
  22. Lee J, Oney M, Frizzell K, Phadnis N, Hollien J. Drosophila melanogaster activating transcription factor 4 regulates glycolysis during endoplasmic reticulum stress. G3 (Bethesda). 2015;5:667-75 pubmed publisher
    ..protein response transcription factor Atf4 was necessary for the up-regulation of glycolytic enzymes and Lactate dehydrogenase (Ldh)...
  23. Martínez Morentin L, Martínez L, Piloto S, Yang H, Schon E, Garesse R, et al. Cardiac deficiency of single cytochrome oxidase assembly factor scox induces p53-dependent apoptosis in a Drosophila cardiomyopathy model. Hum Mol Genet. 2015;24:3608-22 pubmed publisher
    ..Remarkably, apoptosis is enhanced in the muscle and liver of Sco2 knock-out mice, clearly suggesting that cell death is a key feature of the COX deficiencies produced by mutations in Sco genes in humans. ..