Hsp70Bb

Summary

Gene Symbol: Hsp70Bb
Description: Heat-shock-protein-70Bb
Alias: CG31359, DMHSP7D1, Dm-hsp70, Dmel\CG31359, HSP70, Hsp 70, Hsp-70, Hsp70, Hsp70(87C), Hsp70A7d, Hsp70B, Hsp70Bbc, Hsp70Bc, dHsp70, dhsp70, hsp-70, hsp70, hsp70 87C, hsp70B, hsp70Bb, Heat-shock-protein-70Bb, 87C locus, CG31359-PA, Hsp70Bb-PA, heat shock protein 70, heat-shock protein 70, protein 70
Species: fruit fly
Products:     Hsp70Bb

Top Publications

  1. Haney R, Feder M. Contrasting patterns of transposable element insertions in Drosophila heat-shock promoters. PLoS ONE. 2009;4:e8486 pubmed publisher
    ..melanogaster natural populations. Hsp70 promoters exhibit more TE insertions per promoter than all other genesets in the 12 species, similarly to in ..
  2. Folk D, Zwollo P, Rand D, Gilchrist G. Selection on knockdown performance in Drosophila melanogaster impacts thermotolerance and heat-shock response differently in females and males. J Exp Biol. 2006;209:3964-73 pubmed
    ..Finally, we compared temporal profiles of the combined expression of two major heat-shock proteins, HSC70 and HSP70, during heat stress among the females and males from the selected T(KD) lines...
  3. Saunders A, Werner J, Andrulis E, Nakayama T, Hirose S, Reinberg D, et al. Tracking FACT and the RNA polymerase II elongation complex through chromatin in vivo. Science. 2003;301:1094-6 pubmed
    ..Our observations are consistent with FACT being restricted to transcription that involves nucleosome disassembly mechanisms. ..
  4. Winegarden N, Wong K, Sopta M, Westwood J. Sodium salicylate decreases intracellular ATP, induces both heat shock factor binding and chromosomal puffing, but does not induce hsp 70 gene transcription in Drosophila. J Biol Chem. 1996;271:26971-80 pubmed
    ..the induction of HSF binding and heat shock puffs in polytene chromosomes, we found no evidence for increased hsp 70 gene transcription suggesting that chromosomal puffing and gene transcription may be separable events...
  5. Zhang Z, Gilmour D. Pcf11 is a termination factor in Drosophila that dismantles the elongation complex by bridging the CTD of RNA polymerase II to the nascent transcript. Mol Cell. 2006;21:65-74 pubmed
    ..We show that Pcf11 is directly involved in termination in Drosophila. dPcf11 is concentrated at the 3' end of the hsp70 gene in cells, and depletion of dPcf11 with RNAi causes Pol II to readthrough the normal region of termination...
  6. Gong W, Golic K. Genomic deletions of the Drosophila melanogaster Hsp70 genes. Genetics. 2004;168:1467-76 pubmed
    ..melanogaster we set out to delete the Hsp70 genes...
  7. Wheeler J, Bieschke E, Tower J. Muscle-specific expression of Drosophila hsp70 in response to aging and oxidative stress. Proc Natl Acad Sci U S A. 1995;92:10408-12 pubmed
    Induction of Drosophila hsp70 protein was detected during aging in flight muscle and leg muscle in the absence of heat shock, using an hsp70-specific monoclonal antibody, and in transgenic flies containing hsp70-beta-galactosidase fusion ..
  8. Yao J, Munson K, Webb W, Lis J. Dynamics of heat shock factor association with native gene loci in living cells. Nature. 2006;442:1050-3 pubmed
    ..After heat shock, we have visualized the recruitment of RNA polymerase II (Pol II) to native hsp70 gene loci 87A and 87C in real time...
  9. Pelham H, Bienz M. A synthetic heat-shock promoter element confers heat-inducibility on the herpes simplex virus thymidine kinase gene. EMBO J. 1982;1:1473-7 pubmed
    Previous deletion analysis of the Drosophila hsp70 heat-shock promoter has identified a sequence upstream of the TATA box that is required for heat induction...

More Information

Publications76

  1. Krebs R, Feder M. Deleterious consequences of Hsp70 overexpression in Drosophila melanogaster larvae. Cell Stress Chaperones. 1997;2:60-71 pubmed
    We compared transgenic Drosophila larvae varying in hsp70 copy number to assess the consequences of Hsp70 overexpression for growth and development after heat shock...
  2. Tulin A, Spradling A. Chromatin loosening by poly(ADP)-ribose polymerase (PARP) at Drosophila puff loci. Science. 2003;299:560-2 pubmed
    Steroid response and stress-activated genes such as hsp70 undergo puffing in Drosophila larval salivary glands, a local loosening of polytene chromatin structure associated with gene induction...
  3. Rabindran S, Wisniewski J, Li L, Li G, Wu C. Interaction between heat shock factor and hsp70 is insufficient to suppress induction of DNA-binding activity in vivo. Mol Cell Biol. 1994;14:6552-60 pubmed
    ..In addition, elevated concentrations of hsp70 alone could not significantly inhibit induction of the DNA-binding activity of endogenous HSF in cultured rat ..
  4. Shopland L, Lis J. HSF recruitment and loss at most Drosophila heat shock loci is coordinated and depends on proximal promoter sequences. Chromosoma. 1996;105:158-71 pubmed
    ..Finally, the distribution of the heat shock protein HSP70 is examined for its role in regulating the attenuated response of HSF to heat shock.
  5. Simon J, Sutton C, Lobell R, Glaser R, Lis J. Determinants of heat shock-induced chromosome puffing. Cell. 1985;40:805-17 pubmed
    ..Deletion of promoter sequences upstream of position -73 of an hsp70-IacZ hybrid gene was sufficient to abolish puffing...
  6. Feder J, Rossi J, Solomon J, Solomon N, Lindquist S. The consequences of expressing hsp70 in Drosophila cells at normal temperatures. Genes Dev. 1992;6:1402-13 pubmed
    In Drosophila cells, regulatory mechanisms not only act to provide rapid induction of hsp70 during heat shock but also to prevent expression at normal temperatures...
  7. Fernandes M, Xiao H, Lis J. Binding of heat shock factor to and transcriptional activation of heat shock genes in Drosophila. Nucleic Acids Res. 1995;23:4799-804 pubmed
    ..This last result suggests that close proximity of HSEs for cooperative binding of HSF is a result of protein-protein interactions near the point of DNA contact. ..
  8. Auluck P, Meulener M, Bonini N. Mechanisms of Suppression of {alpha}-Synuclein Neurotoxicity by Geldanamycin in Drosophila. J Biol Chem. 2005;280:2873-8 pubmed
    ..The molecular chaperone Hsp70 protects cells from the deleterious effects of alpha-synuclein, indicating a potential therapeutic approach to ..
  9. Weber J, Gilmour D. Genomic footprinting of the hsp70 and histone H3 promoters in Drosophila embryos reveals novel protein-DNA interactions. Nucleic Acids Res. 1995;23:3327-34 pubmed
    The transcriptional potential of the hsp70 heat shock gene promoter is established prior to induction by stress...
  10. Gupta S, Siddique H, Saxena D, Chowdhuri D. Hazardous effect of organophosphate compound, dichlorvos in transgenic Drosophila melanogaster (hsp70-lacZ): induction of hsp70, anti-oxidant enzymes and inhibition of acetylcholinesterase. Biochim Biophys Acta. 2005;1725:81-92 pubmed
    ..Third instar larvae of Drosophila melanogaster transgenic for hsp70 were exposed to 0.1 to 100.0 ppb dichlorvos and 5...
  11. Krebs R, Feder M. Hsp70 and larval thermotolerance in Drosophila melanogaster: how much is enough and when is more too much?. J Insect Physiol. 1998;44:1091-1101 pubmed
    ..g., inducible thermotolerance) whose functional consequences are concentration dependent. We manipulated Hsp70 concentration quantitatively in intact larvae of Drosophila melanogaster to examine its effect on survival, ..
  12. King V, Tower J. Aging-specific expression of Drosophila hsp22. Dev Biol. 1999;207:107-18 pubmed
    ..Aging-specific induction of hsp22 is discussed with regard to current evolutionary theories of aging. ..
  13. Warrick J, Chan H, Gray Board G, Chai Y, Paulson H, Bonini N. Suppression of polyglutamine-mediated neurodegeneration in Drosophila by the molecular chaperone HSP70. Nat Genet. 1999;23:425-8 pubmed
    ..melanogaster model of polyglutamine disease, we show that directed expression of the molecular chaperone HSP70 suppresses polyglutamine-induced neurodegeneration in vivo...
  14. Pelham H. A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene. Cell. 1982;30:517-28 pubmed
    Deletion mutants of the Drosophila hsp 70 heat-shock gene have been assayed in COS cells using a vector that contains an SV40 replication origin...
  15. Eissenberg J, Shilatifard A, Dorokhov N, Michener D. Cdk9 is an essential kinase in Drosophila that is required for heat shock gene expression, histone methylation and elongation factor recruitment. Mol Genet Genomics. 2007;277:101-14 pubmed
    ..b>Hsp 70 mRNA induction by heat shock was attenuated in Cdk9 knockdown larvae...
  16. Sejerkilde M, Sørensen J, Loeschcke V. Effects of cold- and heat hardening on thermal resistance in Drosophila melanogaster. J Insect Physiol. 2003;49:719-26 pubmed
    ..The level of Hsp70 expression induced by a non-lethal cold hardening was examined, showing that cold hardening induced Hsp70 ..
  17. Lebedeva L, Nabirochkina E, Kurshakova M, Robert F, Krasnov A, Evgen ev M, et al. Occupancy of the Drosophila hsp70 promoter by a subset of basal transcription factors diminishes upon transcriptional activation. Proc Natl Acad Sci U S A. 2005;102:18087-92 pubmed
    The presence of general transcription factors and other coactivators at the Drosophila hsp70 gene promoter in vivo has been examined by polytene chromosome immunofluorescence and chromatin immunoprecipitation at endogenous heat-shock ..
  18. Zatsepina O, Velikodvorskaia V, Molodtsov V, Garbuz D, Lerman D, Bettencourt B, et al. A Drosophila melanogaster strain from sub-equatorial Africa has exceptional thermotolerance but decreased Hsp70 expression. J Exp Biol. 2001;204:1869-81 pubmed
    ..of high temperatures, which in Drosophila melanogaster is due in part to the inducible molecular chaperone Hsp70, is only modest in this strain...
  19. Weber J, Taxman D, Lu Q, Gilmour D. Molecular architecture of the hsp70 promoter after deletion of the TATA box or the upstream regulation region. Mol Cell Biol. 1997;17:3799-808 pubmed
    GAGA factor, TFIID, and paused polymerase are present on the hsp70 promoter in Drosophila melanogaster prior to transcriptional activation...
  20. Smith S, Petruk S, Sedkov Y, Cho E, Tillib S, Canaani E, et al. Modulation of heat shock gene expression by the TAC1 chromatin-modifying complex. Nat Cell Biol. 2004;6:162-7 pubmed
    Rapid induction of the Drosophila melanogaster heat shock gene hsp70 is achieved through the binding of heat shock factor (HSF) to heat shock elements (HSEs) located upstream of the transcription start site (reviewed in ref. 3)...
  21. Herold A, Klymenko T, Izaurralde E. NXF1/p15 heterodimers are essential for mRNA nuclear export in Drosophila. RNA. 2001;7:1768-80 pubmed
    ..We conclude that Dm NXF1/p15 heterodimers only (but not NXF2-NXF4) mediate the export of the majority of mRNAs in Drosophila cells and that the other members of the NXF family play more specialized or different roles. ..
  22. Jedlicka P, Mortin M, Wu C. Multiple functions of Drosophila heat shock transcription factor in vivo. EMBO J. 1997;16:2452-62 pubmed
  23. Kopytova D, Orlova A, Krasnov A, Gurskiy D, Nikolenko J, Nabirochkina E, et al. Multifunctional factor ENY2 is associated with the THO complex and promotes its recruitment onto nascent mRNA. Genes Dev. 2010;24:86-96 pubmed publisher
    ..ENY2 and THO arrive on the transcribed region of the hsp70 gene after its activation, and ENY2 plays an important role in THO recruitment...
  24. Rasmussen E, Lis J. In vivo transcriptional pausing and cap formation on three Drosophila heat shock genes. Proc Natl Acad Sci U S A. 1993;90:7923-7 pubmed
    The regulation of many eukaryotic genes occurs at the level of transcriptional elongation. On the uninduced hsp70 gene of Drosophila melanogaster, for example, an RNA polymerase II complex has initiated transcription but has paused early ..
  25. Li B, Weber J, Chen Y, Greenleaf A, Gilmour D. Analyses of promoter-proximal pausing by RNA polymerase II on the hsp70 heat shock gene promoter in a Drosophila nuclear extract. Mol Cell Biol. 1996;16:5433-43 pubmed
    ..RNA polymerase II is paused in a region 20 to 40 nucleotides downstream from the transcription start site of the hsp70 heat shock gene when the gene is not transcriptionally active...
  26. Lis J, Simon J, Sutton C. New heat shock puffs and beta-galactosidase activity resulting from transformation of Drosophila with an hsp70-lacZ hybrid gene. Cell. 1983;35:403-10 pubmed
    A hybrid gene that consists of the Drosophila heat shock gene, hsp70, fused to the E. coli beta-galactosidase gene has been introduced into the Drosophila germline by the P element microinjection method...
  27. Xiao H, Lis J. Germline transformation used to define key features of heat-shock response elements. Science. 1988;239:1139-42 pubmed
    ..Here, the sequence requirements for heat-shock induction are tested by Drosophila germline transformation with an hsp70-lacZ gene fused to a pair of synthetic HSEs...
  28. Kurshakova M, Krasnov A, Kopytova D, Shidlovskii Y, Nikolenko J, Nabirochkina E, et al. SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC. EMBO J. 2007;26:4956-65 pubmed
    ..Importantly, E(y)2 and Xmas-2 knockdown decreases the contact between the heat-shock protein 70 (hsp70) gene loci and the nuclear envelope before and after activation and interferes with transcription...
  29. Maside X, Bartolomé C, Charlesworth B. S-element insertions are associated with the evolution of the Hsp70 genes in Drosophila melanogaster. Curr Biol. 2002;12:1686-91 pubmed
    ..of three members of a heat shock gene family, Hsp70 (Hsp70Aa and Hsp70Ab in polytene chromosome band 87A, and Hsp70Bb in 87C). A PCR-based analysis suggests that the insertions are fixed or at high frequencies in the entire species...
  30. Sørensen J, Loeschcke V. Larval crowding in Drosophila melanogaster induces Hsp70 expression, and leads to increased adult longevity and adult thermal stress resistance. J Insect Physiol. 2001;47:1301-1307 pubmed
    In this study we show for the first time that moderate high larval density induces Hsp70 expression in Drosophila melanogaster larvae. Larval crowding led to both increased mean and maximal longevity in adults of both sexes...
  31. Pompa P, Vecchio G, Galeone A, Brunetti V, Maiorano G, Sabella S, et al. Physical assessment of toxicology at nanoscale: nano dose-metrics and toxicity factor. Nanoscale. 2011;3:2889-97 pubmed publisher
    ..This approach may pave the way to a systematic classification of nanomaterials, leading to important developments in risk assessment and regulatory approval, as well as in a wide range of nanomedicine applications. ..
  32. Corces V, Pellicer A. Identification of sequences involved in the transcriptional control of a Drosophila heat-shock gene. J Biol Chem. 1984;259:14812-7 pubmed
    We have used stable transformation of a cloned Drosophila hsp70 gene into mouse Ltk- cells as an assay system to determine which sequences are involved in the temperature-induced transcription of the gene...
  33. Zhao Y, Sun H, Lu J, Li X, Chen X, Tao D, et al. Lifespan extension and elevated hsp gene expression in Drosophila caused by histone deacetylase inhibitors. J Exp Biol. 2005;208:697-705 pubmed
    ..To further determine if hsp22 and hsp70 expression is correlated with lifespan, and if histone acetylation participates in this process, RNA levels for ..
  34. Zobeck K, Buckley M, Zipfel W, Lis J. Recruitment timing and dynamics of transcription factors at the Hsp70 loci in living cells. Mol Cell. 2010;40:965-75 pubmed publisher
    ..to examine at high temporal resolution the recruitment and dynamics of transcription factors to the inducible Hsp70 loci in individual Drosophila salivary gland nuclei...
  35. Wang H, Kazemi Esfarjani P, Benzer S. Multiple-stress analysis for isolation of Drosophila longevity genes. Proc Natl Acad Sci U S A. 2004;101:12610-5 pubmed
    ..Overexpression of either hsp26 or hsp27 extended the mean lifespan by 30%, and the flies also displayed increased stress resistance. The results demonstrate that multiple-stress screening can be used to identify new longevity genes. ..
  36. Piacentini L, Fanti L, Berloco M, Perrini B, Pimpinelli S. Heterochromatin protein 1 (HP1) is associated with induced gene expression in Drosophila euchromatin. J Cell Biol. 2003;161:707-14 pubmed
    ..phenotype of intense gene activity, we did a detailed analysis of the heat shock-induced expression of the HSP70 encoding gene in larvae with different doses of HP1 and found that HP1 is positively involved in Hsp70 gene ..
  37. Chen B, Shilova V, Zatsepina O, Evgen ev M, Feder M. Location of P element insertions in the proximal promoter region of Hsp70A is consequential for gene expression and correlated with fecundity in Drosophila melanogaster. Cell Stress Chaperones. 2008;13:11-7 pubmed publisher
    ..to cluster-specific quantitative RT-PCR, expression of the Hsp70A cluster genes was typically greater than that of the Hsp70B gene cluster genes, although the latter are more numerous and, in this case, free of P element insertions.
  38. Andrulis E, Guzman E, Döring P, Werner J, Lis J. High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: roles in promoter proximal pausing and transcription elongation. Genes Dev. 2000;14:2635-49 pubmed
    ..These findings provide support for the roles of Spt5 in promoter-associated pausing and of Spt5 and Spt6 in transcriptional elongation in vivo. ..
  39. Papaconstantinou M, Wu Y, Pretorius H, Singh N, Gianfelice G, Tanguay R, et al. Menin is a regulator of the stress response in Drosophila melanogaster. Mol Cell Biol. 2005;25:9960-72 pubmed
    ..The overexpression of menin enhanced the expression of HSP70 in embryos and interfered with its down-regulation during recovery at the normal temperature...
  40. Lerman D, Michalak P, Helin A, Bettencourt B, Feder M. Modification of heat-shock gene expression in Drosophila melanogaster populations via transposable elements. Mol Biol Evol. 2003;20:135-44 pubmed
    We report multiple cases in which disruption of hsp70 regulatory regions by transposable element (TE) insertions underlies natural variation in expression of the stress-inducible molecular chaperone Hsp70 in Drosophila melanogaster...
  41. Benjamin L, Gilmour D. Nucleosomes are not necessary for promoter-proximal pausing in vitro on the Drosophila hsp70 promoter. Nucleic Acids Res. 1998;26:1051-5 pubmed
    ..A study of the human hsp70 promoter showed that a nucleosome positioned downstream from the transcription start was a key component in ..
  42. Nazir A, Saxena D, Kar Chowdhuri D. Induction of hsp70 in transgenic Drosophila: biomarker of exposure against phthalimide group of chemicals. Biochim Biophys Acta. 2003;1621:218-25 pubmed
    ..This led us to explore the possibility of using expression of one of the major stress genes, hsp70, in Drosophila as a biomarker against phthalimide group of chemicals, which may accordingly provide an early ..
  43. Rasmussen E, Lis J. Short transcripts of the ternary complex provide insight into RNA polymerase II elongational pausing. J Mol Biol. 1995;252:522-35 pubmed
    Expression of the hsp70 gene of Drosophila melanogaster is controlled at the level of transcript elongation...
  44. Perisic O, Xiao H, Lis J. Stable binding of Drosophila heat shock factor to head-to-head and tail-to-tail repeats of a conserved 5 bp recognition unit. Cell. 1989;59:797-806 pubmed
    ..We propose that trimers of HSF bind to DNA and that the number of HSF subunits in direct contact with DNA is determined by the number of correctly positioned 5 bp recognition units. ..
  45. Lerman D, Feder M. Naturally occurring transposable elements disrupt hsp70 promoter function in Drosophila melanogaster. Mol Biol Evol. 2005;22:776-83 pubmed
    ..the functional consequences of these TE insertions, we created constructs with either TE-bearing or TE-lacking hsp70 promoters fused to a luciferase reporter gene and assayed luciferase luminescence in transiently transfected ..
  46. Smith E, Lee M, Winter B, Droz N, Eissenberg J, Shiekhattar R, et al. Drosophila UTX is a histone H3 Lys27 demethylase that colocalizes with the elongating form of RNA polymerase II. Mol Cell Biol. 2008;28:1041-6 pubmed
    ..Furthermore, heat shock induction results in the recruitment of dUTX to the hsp70 gene, like that of several other Pol II elongation factors...
  47. Ni Z, Schwartz B, Werner J, Suarez J, Lis J. Coordination of transcription, RNA processing, and surveillance by P-TEFb kinase on heat shock genes. Mol Cell. 2004;13:55-65 pubmed
    ..Brief treatment of Drosophila cells with flavopiridol leads to a reduction in the accumulation of induced hsp70 and hsp26 RNAs...
  48. Fujikake N, Nagai Y, Popiel H, Okamoto Y, Yamaguchi M, Toda T. Heat shock transcription factor 1-activating compounds suppress polyglutamine-induced neurodegeneration through induction of multiple molecular chaperones. J Biol Chem. 2008;283:26188-97 pubmed publisher
    ..3% rescue), indicating that 17-AAG is widely effective against various polyQ diseases. 17-AAG induced Hsp70, Hsp40, and Hsp90 expression in a dose-dependent manner, and the expression levels correlated with its therapeutic ..
  49. O BRIEN T, Lis J. RNA polymerase II pauses at the 5' end of the transcriptionally induced Drosophila hsp70 gene. Mol Cell Biol. 1991;11:5285-90 pubmed
    An RNA polymerase II molecule is associated with the 5' end of the Drosophila melanogaster hsp70 gene under non-heat shock conditions...
  50. Lee H, Kraus K, Wolfner M, Lis J. DNA sequence requirements for generating paused polymerase at the start of hsp70. Genes Dev. 1992;6:284-95 pubmed
    RNA polymerase II is transcriptionally engaged but paused approximately 25 nucleotides from the start site of the hsp70 gene of Drosophila melanogaster in uninduced (non-heat-shocked) flies...
  51. Landis G, Abdueva D, Skvortsov D, Yang J, Rabin B, Carrick J, et al. Similar gene expression patterns characterize aging and oxidative stress in Drosophila melanogaster. Proc Natl Acad Sci U S A. 2004;101:7663-8 pubmed
    ..Immune reporter expression in young flies was partially predictive of remaining life span, suggesting their potential as biomonitors of aging. ..
  52. Singh M, Reddy M, Mathur N, Saxena D, Chowdhuri D. Induction of hsp70, hsp60, hsp83 and hsp26 and oxidative stress markers in benzene, toluene and xylene exposed Drosophila melanogaster: role of ROS generation. Toxicol Appl Pharmacol. 2009;235:226-43 pubmed publisher
    ..Third instar larvae of D. melanogaster transgenic for hsp70, hsp83 and hsp26 and Oregon R(+) strain were exposed to 1.0-100...
  53. Amin J, Ananthan J, Voellmy R. Key features of heat shock regulatory elements. Mol Cell Biol. 1988;8:3761-9 pubmed
    ..Second, the nucleotides at the two positions immediately upstream from GAA segments played an important role in defining the competence of regulatory elements. ..
  54. Bettencourt B, Feder M. Hsp70 duplication in the Drosophila melanogaster species group: how and when did two become five?. Mol Biol Evol. 2001;18:1272-82 pubmed publisher
    To determine how the modern copy number (5) of hsp70 genes in Drosophila melanogaster evolved, we localized the duplication events that created the genes in the phylogeny of the melanogaster group, examined D...
  55. O BRIEN T, Wilkins R, Giardina C, Lis J. Distribution of GAGA protein on Drosophila genes in vivo. Genes Dev. 1995;9:1098-110 pubmed
    ..GAGA protein has been implicated in the transcription of numerous Drosophila genes, including hsp70, hsp26, actin 5C, and Ubx...
  56. Takahashi A, Tsaur S, Coyne J, Wu C. The nucleotide changes governing cuticular hydrocarbon variation and their evolution in Drosophila melanogaster. Proc Natl Acad Sci U S A. 2001;98:3920-5 pubmed
    ..Whether this CH variation could be a component of the sexual isolation between Zimbabwe and other cosmopolitan populations remains an interesting and unresolved question. ..
  57. Yang J, Tower J. Expression of hsp22 and hsp70 transgenes is partially predictive of drosophila survival under normal and stress conditions. J Gerontol A Biol Sci Med Sci. 2009;64:828-38 pubmed publisher
    Drosophila Hsp70 is a highly conserved molecular chaperone with numerous cytoplasmic targets. Hsp22 is an alpha-crystallin-related chaperone (small hsp) that localizes to the mitochondrial matrix...
  58. Shilova V, Garbuz D, Myasyankina E, Chen B, Evgen ev M, Feder M, et al. Remarkable site specificity of local transposition into the Hsp70 promoter of Drosophila melanogaster. Genetics. 2006;173:809-20 pubmed
    ..have exploited a local transposition technique and Drosophila melanogaster strains with EPgy2 insertions near the Hsp70 gene cluster at 87A7 to produce numerous novel EPgy2 insertions into these Hsp70 genes...
  59. Feder M, Cartaño N, Milos L, Krebs R, Lindquist S. Effect of engineering Hsp70 copy number on Hsp70 expression and tolerance of ecologically relevant heat shock in larvae and pupae of Drosophila melanogaster. J Exp Biol. 1996;199:1837-44 pubmed
    To determine how the accumulation of the major Drosophila melanogaster heat-shock protein, Hsp70, affects inducible thermotolerance in larvae and pupae, we have compared two sister strains generated by site-specific homologus ..
  60. Takahashi K, Rako L, Takano Shimizu T, Hoffmann A, Lee S. Effects of small Hsp genes on developmental stability and microenvironmental canalization. BMC Evol Biol. 2010;10:284 pubmed publisher
    ..This novel finding may lead to a better understanding of non-Hsp90 molecular mechanisms controlling variability in morphological traits. ..
  61. Buszczak M, Spradling A. The Drosophila P68 RNA helicase regulates transcriptional deactivation by promoting RNA release from chromatin. Genes Dev. 2006;20:977-89 pubmed
    ..Full-length hsp70 mRNA accumulates in the nucleus near its sites of transcription following heat shock of p68 homozygotes, and hsp70 ..
  62. Gong W, Golic K. Loss of Hsp70 in Drosophila is pleiotropic, with effects on thermotolerance, recovery from heat shock and neurodegeneration. Genetics. 2006;172:275-86 pubmed
    ..In Drosophila melanogaster the Hsp70 chaperone dominates the profile of protein synthesis during the heat-shock response...
  63. Rougvie A, Lis J. Postinitiation transcriptional control in Drosophila melanogaster. Mol Cell Biol. 1990;10:6041-5 pubmed
    Drosophila hsp70 genes have an RNA polymerase II molecule paused at their 5' ends in uninduced cells. In this study we have shown that this pausing also occurs on other heat shock and constitutively expressed genes...
  64. Welte M, Tetrault J, Dellavalle R, Lindquist S. A new method for manipulating transgenes: engineering heat tolerance in a complex, multicellular organism. Curr Biol. 1993;3:842-53 pubmed
    ..a novel thermotolerance assay for Drosophila embryos, we investigated the role of the 70 kD heat-shock protein, Hsp 70, in thermotolerance...
  65. Grover D, Yang J, Tavare S, Tower J. Simultaneous tracking of fly movement and gene expression using GFP. BMC Biotechnol. 2008;8:93 pubmed publisher
    ..eyeless/Pax6 reporter expression had a 12 hr period that correlated with fly activity levels. hsp70 and hsp22 gene reporters were induced during fly aging in circadian patterns (24 hr and 18 hr periods, ..
  66. Krebs R, Feder M. Tissue-specific variation in Hsp70 expression and thermal damage in Drosophila melanogaster larvae. J Exp Biol. 1997;200:2007-15 pubmed
    All tissues of larval Drosophila melanogaster express Hsp70, the major heat-shock protein of this species, after both mild (36 degrees C) and severe (38.5 degrees C) heat shock...
  67. Lis J. Promoter-associated pausing in promoter architecture and postinitiation transcriptional regulation. Cold Spring Harb Symp Quant Biol. 1998;63:347-56 pubmed