Hsp27

Summary

Gene Symbol: Hsp27
Description: Heat shock protein 27
Alias: 27K, CG4466, Dhsp27, DmHSP27, DmHsp27, Dmel\CG4466, HSP27, HSP28, Hsp 27, Hsp28, anon-WO0140519.69, hsp 27, hsp-27, hsp26, hsp27, hsp27/26, hsp28, small hsp locus 67B, CG4466-PA, Hsp 27, Hsp27-PA, heat shock protein 237, heat shock protein 27, heat shock protein hsp27, protein 27
Species: fruit fly

Top Publications

  1. pmc The Hsp27 gene is not required for Drosophila development but its activity is associated with starvation resistance
    Xinming Hao
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 2131, USA
    Cell Stress Chaperones 12:364-72. 2007
  2. ncbi The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily
    M R Koelle
    Department of Biochemistry, Stanford University Medical Center, California 94305
    Cell 67:59-77. 1991
  3. ncbi HSF recruitment and loss at most Drosophila heat shock loci is coordinated and depends on proximal promoter sequences
    L S Shopland
    Section of Biochemistry, Molecular and Cell Biology, 417 Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    Chromosoma 105:158-71. 1996
  4. ncbi Germline transformation used to define key features of heat-shock response elements
    H Xiao
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853
    Science 239:1139-42. 1988
  5. ncbi GST-Induced dimerization of DNA-binding domains alters characteristics of their interaction with DNA
    A Niedziela-Majka
    Biochemistry and Biotechnology, Division of Biochemistry, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, Wroclaw, 50 370, Poland
    Protein Expr Purif 14:208-20. 1998
  6. ncbi Polarity of the ecdysone receptor complex interaction with the palindromic response element from the hsp27 gene promoter
    A Niedziela-Majka
    Institute of Organic Chemistry, Division of Biochemistry, Wroctaw University of Technology, Poland
    Eur J Biochem 267:507-19. 2000
  7. ncbi Analysis of Usp DNA binding domain targeting reveals critical determinants of the ecdysone receptor complex interaction with the response element
    I Grad
    Institute of Organic Chemistry, Biochemistry and Biotechnology, Division of Biochemistry, Wroclaw University of Technology, Poland
    Eur J Biochem 268:3751-8. 2001
  8. pmc Phylogeny disambiguates the evolution of heat-shock cis-regulatory elements in Drosophila
    Sibo Tian
    Department of Organismal Biology and Anatomy, University of Chicago, Chicago, Illinois, United States of America
    PLoS ONE 5:e10669. 2010
  9. ncbi The DNA-binding domain of the ultraspiracle drives deformation of the response element whereas the DNA-binding domain of the ecdysone receptor is responsible for a slight additional change of the preformed structure
    Piotr Dobryszycki
    Division of Biochemistry, Institute of Organic Chemistry, Biochemistry and Biotechnology, Wrocław University of Technology, Wybrzeze Wyspianskiego 27, 50 370 Wrocław, Poland
    Biochemistry 45:668-75. 2006
  10. ncbi Induction of the heat shock pathway during hypoxia requires regulation of heat shock factor by hypoxia-inducible factor-1
    Nathan A Baird
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    J Biol Chem 281:38675-81. 2006

Scientific Experts

  • R M Tanguay
  • Roger F Duncan
  • J Frydenberg
  • Hervé Colinet
  • M I Galindo
  • M Lehmann
  • L Nover
  • Brian R Bettencourt
  • Tomasz Krusiński
  • Piotr Dobryszycki
  • Andrzej Ozyhar
  • A Niedziela-Majka
  • Horng Dar Wang
  • Grzegorz Rymarczyk
  • S Schauer
  • Benjamin Timakov
  • Sibo Tian
  • Joshua M Beatty
  • R Marin
  • Kazuko Hanyu-Nakamura
  • Pin Chao Liao
  • Xinming Hao
  • Jie Yao
  • Nathan A Baird
  • P Mehlen
  • Ping Zhang
  • Marian Kochman
  • Iwona Grad
  • M Kochman
  • A Ozyhar
  • Ruhi Ahmed
  • S Michaud
  • Gretchen L Geiger-Thornsberry
  • Shun Sawatsubashi
  • Jeroen Poels
  • Li Chen
  • J T Lis
  • Young Woo Seo
  • I Grad
  • O G Zatsepina
  • S Braun
  • M Spindler-Barth
  • A Azoitei
  • R Kurapati
  • Martin E Feder
  • Robert A Haney
  • Takehiko Ogura
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  • Saya Ito
  • Marie Marthe Suner
  • Yue Zhao

Detail Information

Publications88

  1. pmc The Hsp27 gene is not required for Drosophila development but its activity is associated with starvation resistance
    Xinming Hao
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 2131, USA
    Cell Stress Chaperones 12:364-72. 2007
    ..We investigated a fruit fly gene encoding a small heat shock protein, Hsp27, to determine if it functions in stress response of Drosophila melanogaster. A knockout Hsp27 allele was generated...
  2. ncbi The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily
    M R Koelle
    Department of Biochemistry, Stanford University Medical Center, California 94305
    Cell 67:59-77. 1991
    ..As expected, EcR is nuclear and found in all ecdysone target tissues examined. Furthermore, the EcR gene is expressed at each developmental stage marked by a pulse of ecdysone...
  3. ncbi HSF recruitment and loss at most Drosophila heat shock loci is coordinated and depends on proximal promoter sequences
    L S Shopland
    Section of Biochemistry, Molecular and Cell Biology, 417 Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    Chromosoma 105:158-71. 1996
    ..Finally, the distribution of the heat shock protein HSP70 is examined for its role in regulating the attenuated response of HSF to heat shock...
  4. ncbi Germline transformation used to define key features of heat-shock response elements
    H Xiao
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853
    Science 239:1139-42. 1988
    ..These and additional analyses indicate that the heat-shock regulatory element includes sequences beyond the 14-base pair HSE and may be better described as a dimer of a 10-base pair sequence, NTTCNNGAAN...
  5. ncbi GST-Induced dimerization of DNA-binding domains alters characteristics of their interaction with DNA
    A Niedziela-Majka
    Biochemistry and Biotechnology, Division of Biochemistry, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, Wroclaw, 50 370, Poland
    Protein Expr Purif 14:208-20. 1998
    ..that UspDBD exhibited higher affinity than EcRDBD toward a 20-hydroxyecdysone response element from the Drosophila hsp 27 gene (hsp 27pal)...
  6. ncbi Polarity of the ecdysone receptor complex interaction with the palindromic response element from the hsp27 gene promoter
    A Niedziela-Majka
    Institute of Organic Chemistry, Division of Biochemistry, Wroctaw University of Technology, Poland
    Eur J Biochem 267:507-19. 2000
    ..EcR and Usp DNA-binding domains (EcRDBD and UspDBD, respectively) with the palindromic response element from the hsp27 gene promoter (hsp27pal)...
  7. ncbi Analysis of Usp DNA binding domain targeting reveals critical determinants of the ecdysone receptor complex interaction with the response element
    I Grad
    Institute of Organic Chemistry, Biochemistry and Biotechnology, Division of Biochemistry, Wroclaw University of Technology, Poland
    Eur J Biochem 268:3751-8. 2001
    ..a specific anchor that preferentially binds the 5' half-site of the pseudo-palindromic response element from the hsp27 gene promoter and thus locates the heterocomplex in the defined orientation...
  8. pmc Phylogeny disambiguates the evolution of heat-shock cis-regulatory elements in Drosophila
    Sibo Tian
    Department of Organismal Biology and Anatomy, University of Chicago, Chicago, Illinois, United States of America
    PLoS ONE 5:e10669. 2010
    ..The broad dimensions of variation uncovered are particularly important as they suggest a substantial challenge for functional studies...
  9. ncbi The DNA-binding domain of the ultraspiracle drives deformation of the response element whereas the DNA-binding domain of the ecdysone receptor is responsible for a slight additional change of the preformed structure
    Piotr Dobryszycki
    Division of Biochemistry, Institute of Organic Chemistry, Biochemistry and Biotechnology, Wrocław University of Technology, Wybrzeze Wyspianskiego 27, 50 370 Wrocław, Poland
    Biochemistry 45:668-75. 2006
    ..resonance energy transfer (FRET) to study the topology of the natural pseudopalindromic element from the hsp27 gene (hsp27pal) in complex with the DNA-binding domains of Usp and EcR (UspDBD and EcRDBD, respectively)...
  10. ncbi Induction of the heat shock pathway during hypoxia requires regulation of heat shock factor by hypoxia-inducible factor-1
    Nathan A Baird
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    J Biol Chem 281:38675-81. 2006
    ..This cross-regulation represents a mechanism by which the low oxygen response pathway has assimilated complex new functions by regulating the key transcriptional activator of the heat shock pathway...
  11. ncbi Regulation of heat shock gene induction and expression during Drosophila development
    S Michaud
    Laboratoire de génétique cellulaire et developpmentale, RSVS, Universite Laval, Sainte Foy, Quebec, Canada
    Cell Mol Life Sci 53:104-13. 1997
    ..During development, each of the four members of the small heat shock protein family (Hsp27, Hsp26 Hsp23 and Hsp22), which are coordinately induced in response to a heat stress, shows a specific pattern of ..
  12. ncbi Characterization of a specific ecdysteroid receptor-DNA complex reveals common properties for invertebrate and vertebrate hormone-receptor/DNA interactions
    A Oyhar
    Ruhr Universitat Bochum, Lehrstuhl für Biochemie, Federal Republic of Germany
    Eur J Biochem 200:329-35. 1991
    ..between Drosophila melanogaster ecdysteroid receptor and the 20-hydroxy-ecdysone responsive element of the hsp27 gene promoter region was characterized in terms of complex formation, saturation of DNA binding and the apparent ..
  13. pmc Tissue-specific expression of the heat shock protein HSP27 during Drosophila melanogaster development
    D Pauli
    Department of Molecular Biology, University of Geneva, Switzerland
    J Cell Biol 111:817-28. 1990
    The alpha-crystallin-related heat shock (stress) protein hsp27 is expressed in absence of heat shock during Drosophila melanogaster development...
  14. pmc A common ancestor DNA motif for invertebrate and vertebrate hormone response elements
    E Martinez
    Institut de Biologie Animale, Universite de Lausanne, Batiment de Biologie, Switzerland
    EMBO J 10:263-8. 1991
    The ecdysone-responsive DNA sequence of the Drosophila hsp27 gene promoter contains four direct and inverted repeats reminiscent of those that compose the vertebrate palindromic estrogen response element (ERE) and the thyroid hormone/..
  15. pmc Binding of heat shock factor to and transcriptional activation of heat shock genes in Drosophila
    M Fernandes
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853, USA
    Nucleic Acids Res 23:4799-804. 1995
    ..This last result suggests that close proximity of HSEs for cooperative binding of HSF is a result of protein-protein interactions near the point of DNA contact...
  16. ncbi A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene
    H R Pelham
    Cell 30:517-28. 1982
    ..It seems that the upstream element of the hsp 70 promoter is analogous to that of other promoters, but is only functional in heat-shocked cells...
  17. doi The effect of neuronal expression of heat shock proteins 26 and 27 on lifespan, neurodegeneration, and apoptosis in Drosophila
    Pin Chao Liao
    Department of Life Science and Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC
    Biochem Biophys Res Commun 376:637-41. 2008
    ..Our previous study reported that ubiquitous expression of hsp26 or hsp27 extended Drosophila lifespan...
  18. ncbi Small stress proteins as novel regulators of apoptosis. Heat shock protein 27 blocks Fas/APO-1- and staurosporine-induced cell death
    P Mehlen
    Laboratoire du Stress Cellulaire, Centre de Genetique Moleculaire et Cellulaire, CNRS UMR 5534, Universite Claude Bernard Lyon I, 69622 Villeurbanne, France
    J Biol Chem 271:16510-4. 1996
    ..also report that the expression of small stress proteins from different species, such as human hsp27, Drosophila Dhsp27, or human alphaB-crystallin, confers resistance to apoptotic cell death induced by staurosporine, a protein kinase ..
  19. pmc Distribution of B52 within a chromosomal locus depends on the level of transcription
    D T Champlin
    Section of Biochemistry, Cornell University, Ithaca, New York 14853
    Mol Biol Cell 5:71-9. 1994
    ..We suggest that B52 tracks to chromatin fibers that are folding or unfolding, and we discuss this in light of B52's proposed roles in pre-mRNA splicing and control...
  20. ncbi RNA polymerase II transcribes all of the heat shock induced genes of Drosophila melanogaster
    J J Bonner
    Chromosoma 85:93-108. 1982
    ..Thus the protein-coding and non-protein-coding heat shock-induced RNAs are transcribed by this polymerase specifically. We have also identified several non-puffed chromosomal sites at which RNA synthesis is induced by heat shock...
  21. pmc Ecdysone regulation of the Drosophila Sgs-4 gene is mediated by the synergistic action of ecdysone receptor and SEBP 3
    M Lehmann
    Institut fur Genetik, Freien Universitat Berlin, Germany
    EMBO J 14:716-26. 1995
    ..Thus, the available data suggest that induction of Sgs-4, and possibly other 'intermolt genes', is a combination of a primary and a secondary response to the hormone...
  22. ncbi Two new regulatory elements controlling the Drosophila Sgs-3 gene are potential ecdysone receptor and fork head binding sites
    M Lehmann
    Institut für Genetik der Freien Universität Berlin, Germany
    Mech Dev 62:15-27. 1997
    ..Since also the Sgs-4 gene is controlled by a functional EcR/USP binding site, a direct participation of EcR/USP in the formation of regulatory complexes may be of general importance for the hormonal control of Sgs genes...
  23. ncbi DNA binding and transactivation characteristics of the mosquito ecdysone receptor-Ultraspiracle complex
    S F Wang
    Programs in Genetics, Cell and Molecular Biology, Departments of Entomology, Michigan State University, East Lansing, Michigan 48824, USA
    J Biol Chem 273:27531-40. 1998
    ..Taken together, these analyses predict broad variability in the EcREs of mosquito ecdysone-responsive genes...
  24. doi Influence of hormone response elements (HREs) on ecdysteroid receptor concentration
    S Schauer
    Institute of General Zoology and Endocrinology, Ulm University, Albert Einstein Allee 11, Ulm, Germany
    Insect Mol Biol 20:701-11. 2011
    ..The complex regulation of receptor protein concentration offers an additional opportunity to regulate transcriptional activity in an isoform- and target cell-specific manner and allows the temporal limitation of hormone action...
  25. ncbi FOR, a novel orphan nuclear receptor related to farnesoid X receptor
    Young Woo Seo
    Hormone Research Center, Department of Biology, Chonnam National University, Kwangju 500 757, Republic of Korea
    J Biol Chem 277:17836-44. 2002
    ..Finally, although FORs do not exhibit constitutive transcriptional activity, frog gallbladder extract significantly augments the transcriptional activities of FORs...
  26. ncbi Functional and comparative analysis of two distinct ecdysteroid-responsive gene expression constructs in Drosophila S2 cells
    Jeroen Poels
    Laboratory for Developmental Physiology, Genomics and Proteomics, Zoological Institute, Naamsestraat 59, B 3000 Leuven, Belgium
    Insect Biochem Mol Biol 34:451-8. 2004
    ..In addition, the combination of an ecdysone switch with promoters that possess different structural elements can provide novel insights into ecdysteroid-induced transcription in an insect cell line...
  27. ncbi Inhibition of Hsp90 function delays and impairs recovery from heat shock
    Roger F Duncan
    Department of Molecular Pharmacology and Toxicology, University of Southern California School of Pharmacy, Los Angeles, CA, USA
    FEBS J 272:5244-56. 2005
    ..Impaired function of either component is sufficient to alter the heat shock response...
  28. pmc Dual FRET assay for detecting receptor protein interaction with DNA
    Tomasz Krusiński
    Wroclaw University of Technology, Faculty of Chemistry, Division of Biochemistry, Wybrzeze Wyspianskiego 27, 50 370 Wrocław, Poland
    Nucleic Acids Res 38:e108. 2010
    ..Usp) and ecdysone receptor (EcR) from Drosophila melanogaster and a response element from the promoter of the hsp27 gene. The response element consists of two binding sites (half-sites) for the DNA binding domains (DBDs)...
  29. pmc Recovery of protein synthesis after heat shock: prior heat treatment affects the ability of cells to translate mRNA
    N S Petersen
    Proc Natl Acad Sci U S A 78:1708-11. 1981
    ..This indicates that the pretreatment affects translation directly rather than messenger concentration. We also observe selective translation of heat shock messages and some 25 degrees C messages during recovery from heat shock...
  30. ncbi Translational regulation of Hsp90 mRNA. AUG-proximal 5'-untranslated region elements essential for preferential heat shock translation
    Ruhi Ahmed
    University of Southern California School of Pharmacy, Department of Molecular Pharmacology and Toxicology and School of Medicine, Los Angeles, California 90033, USA
    J Biol Chem 279:49919-30. 2004
    ..We present a model to account for Hsp90 mRNA translation, incorporating results indicating that heat shock inhibits eIF4F activity, and that Hsp90 mRNA translation is sensitive to eIF4F inactivation...
  31. ncbi Differential expression of heat shock proteins in Drosophila embryonic cells following metal ion exposure
    N Bournias-Vardiabasis
    Division of Biology, California State University, San Bernadino 92407
    Exp Cell Res 189:177-82. 1990
    ..None of the metals tested induced only the 26- and 27-K proteins. These results suggest that there exist different regulatory mechanisms responsible for the heat shock response...
  32. ncbi Purification of Drosophila melanogaster ultraspiracle protein and analysis of its A/B region-dependent dimerization behavior in vitro
    Grzegorz Rymarczyk
    Institute of Organic Chemistry, Biochemistry and Biotechnology, Division of Biochemistry, Wrocław University of Technology, Wybrzeze Wyspianskiego 27, 50 370 Wrocław, Poland
    Biol Chem 384:59-69. 2003
    ..It was demonstrated that the recombinant receptor specifically binds the ecdysone response element of the hsp27 gene promoter (hsp27EcRE)...
  33. ncbi Induction of the early-late Ddc gene during Drosophila metamorphosis by the ecdysone receptor
    Li Chen
    Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada T6G 2E9
    Mech Dev 114:95-107. 2002
    ..the Ddc EcRE for the ecdysone receptor complex was at least four-fold less than that of the canonical EcRE of the hsp27 gene...
  34. pmc Timing and targeting of P-element local transposition in the male germline cells of Drosophila melanogaster
    Benjamin Timakov
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 2131, USA
    Genetics 160:1011-22. 2002
    ..Our analysis shows that local transposition of the P element is highly regulated, displaying a cell-type specificity and a target specificity...
  35. ncbi A Drosophila melanogaster strain from sub-equatorial Africa has exceptional thermotolerance but decreased Hsp70 expression
    O G Zatsepina
    Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street 32, 117984 Moscow, Russia
    J Exp Biol 204:1869-81. 2001
    ..We hypothesize that the reduced Hsp70 expression in a Drosophila melanogaster strain living chronically at intermediate temperatures may represent an evolved suppression of the deleterious phenotypes of Hsp70...
  36. ncbi Prompt heat-shock and heat-shifted proteins associated with the nuclear matrix-intermediate filament scaffold in Drosophila melanogaster cells
    D A Ornelles
    Department of Biology, Princeton University, New Jersey 08544
    J Cell Sci 95:393-404. 1990
    ..The prompt HS proteins can be distinguished clearly from heat-shifted proteins by several criteria. Also, the prompt HS proteins are distinct from the heat-insensitive viral proteins of a persistent virus (HPS-1)...
  37. ncbi Puffs and gene regulation--molecular insights into the Drosophila ecdysone regulatory hierarchy
    C S Thummel
    Howard Hughes Medical Institute, Department of Human Genetics, University of Utah Medical Center, Salt Lake City 84132
    Bioessays 12:561-8. 1990
    ..Proteins encoded by two of the early genes bind to both early and late ecdysone-induced puffs, implying that they are key regulators in the hierarchy...
  38. ncbi Aging-specific expression of Drosophila hsp22
    V King
    Department of Biological Sciences, University of Southern California, University Park, Los Angeles, California, 90089 1340, USA
    Dev Biol 207:107-18. 1999
    ..Aging-specific induction of hsp22 is discussed with regard to current evolutionary theories of aging...
  39. ncbi Ecdysteroid-regulated heat-shock gene expression during Drosophila melanogaster development
    S R Thomas
    Dev Biol 115:434-8. 1986
    ..These results are consistent with the induction of the hsp 83, 28, and 26 genes by ecdysteroid at several developmental stages...
  40. ncbi Systematic gain-of-function genetics in Drosophila
    P Rørth
    Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210, USA
    Development 125:1049-57. 1998
    ..These studies demonstrate the ability of the modular misexpression system to identify developmentally important genes and suggest that it will be generally useful for genetic interaction screens...
  41. ncbi Cell-specific expression and heat-shock induction of Hsps during spermatogenesis in Drosophila melanogaster
    S Michaud
    CHUL Research Center CHUQ, Universite Laval, Ste Foy, Quebec, Canada
    J Cell Sci 110:1989-97. 1997
    The developmental and heat-shock-induced expression of two small heat-shock proteins (Hsp23 and Hsp27) was investigated during spermatogenesis in Drosophila melanogaster...
  42. ncbi Developmental expression of Drosophila melanogaster small heat-shock proteins
    C Haass
    ZMBH Molekulare Genetik, University of Heidelberg, FRG
    J Cell Sci 96:413-8. 1990
    ....
  43. ncbi Increased hsp22 RNA levels in Drosophila lines genetically selected for increased longevity
    R Kurapati
    Department of Biological Sciences, University of Southern California, Los Angeles 90089 1340, USA
    J Gerontol A Biol Sci Med Sci 55:B552-9. 2000
    ..Thus increased hsp22 and hsp23 RNA levels correlate with the increased life span and increased stress resistance of the genetically selected O lines...
  44. ncbi Quantitative trait loci affecting natural variation in Drosophila longevity
    Gretchen L Geiger-Thornsberry
    Department of Genetics, Box 7614, North Carolina State University, Raleigh 27695, USA
    Mech Ageing Dev 125:179-89. 2004
    ..Quantitative complementation tests are therefore useful for identifying QTL contributing to segregating genetic variation in life span in nature...
  45. ncbi Ecdysone receptor-dependent gene regulation mediates histone poly(ADP-ribosyl)ation
    Shun Sawatsubashi
    The Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Biochem Biophys Res Commun 320:268-72. 2004
    ..Our results indicated that EcR-mediated gene regulation may be coupled with chromatin modification through poly(ADP-ribosyl)ation...
  46. pmc Genetic mapping of the coding regions for three heat-shock proteins in Drosophila melanogaster
    N S Peterson
    Genetics 92:891-902. 1979
    ..All three map to a region on chromosome 3L that includes only one heat-shock puff, designated as 67B. The results imply that the genes coding for at least three heat-shock proteins are included within the 67B region...
  47. doi Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system--a basis for screening novel insecticidal candidates
    Joshua M Beatty
    Center for Biotechnology, Genomics, and Health Research, University of North Carolina at Greensboro, NC 27402, USA
    FEBS J 276:3087-98. 2009
    ..decemlineata USP than with D. melanogaster USP. Generally, the species-based differences validate the use of the cell culture assay screen for novel agonists and potentiators as species-targeted insecticidal candidates...
  48. ncbi Messenger RNA in heat-shocked Drosophila cells
    A Spradling
    J Mol Biol 109:559-87. 1977
  49. ncbi Expression of Drosophila's 27 kDa heat shock protein into rodent cells confers thermal resistance
    E Rollet
    Laboratoire de Genetique Cellulaire et Moleculaire, CHUL, Ste Foy, QC, Canada
    Biochem Biophys Res Commun 185:116-20. 1992
    The role of hsp27, one of Drosophila melanogaster's small heat shock proteins, in the process of thermotolerance was investigated...
  50. pmc Drosophila Pgc protein inhibits P-TEFb recruitment to chromatin in primordial germ cells
    Kazuko Hanyu-Nakamura
    Laboratory for Germline Development, RIKEN Center for Developmental Biology, Kobe, Hyogo 650 0047, Japan
    Nature 451:730-3. 2008
    ..Thus, inhibition of P-TEFb is probably a common mechanism during germ cell specification in the disparate organisms C. elegans and Drosophila...
  51. ncbi Intranuclear distribution and local dynamics of RNA polymerase II during transcription activation
    Jie Yao
    School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    Mol Cell 28:978-90. 2007
    ..Pol II at highly transcribed developmental loci exhibits dynamics resembling combinations of these Hsp70 transcription modes...
  52. ncbi Inactivation of mRNA cap-binding protein complex in Drosophila melanogaster embryos under heat shock
    J M Zapata
    Centro de Biologia Molecular, Consejo Superior de Investigaciones Cientificas, Madrid, Spain
    J Biol Chem 266:16007-14. 1991
    ..Together, the above results suggest that some modification leading to the disruption of Drosophila CBP complex may account, at least to some extent, for the mRNA discrimination established in heat-shocked Drosophila embryos...
  53. pmc Genes for Drosophila small heat shock proteins are regulated differently by ecdysterone
    J Amin
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101
    Mol Cell Biol 11:5937-44. 1991
    Genes for small heat shock proteins (hsp27 to hsp22) are activated in late third-instar larvae of Drosophila melanogaster in the absence of heat stress...
  54. pmc Ecdysterone regulatory elements function as both transcriptional activators and repressors
    L Dobens
    Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755
    Mol Cell Biol 11:1846-53. 1991
    ..23-bp ecdysterone regulatory element (EcRE), derived from the upstream region of the Drosophila melanogaster hsp27 gene, was inserted adjacent to the herpes simplex virus thymidine kinase promoter fused to a bacterial gene for ..
  55. pmc Multiple-stress analysis for isolation of Drosophila longevity genes
    Horng Dar Wang
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 101:12610-5. 2004
    ..Two of these, hsp26 and hsp27, were chosen to test for their effects on lifespan by generating transgenic lines and by using the ..
  56. pmc Cell-specific heat-shock induction of Hsp23 in the eye of Drosophila melanogaster
    R Marin
    Centre deRecherche du CHUL, Universite Laval, Ste Foy, Quebec, Canada
    Cell Stress Chaperones 1:40-6. 1996
    The expression of two small heat shock proteins (sHsp), Hsp23 and Hsp27, was examined by immunological approaches in the eye of Drosophila melanogaster...
  57. pmc Co-localization of Polycomb protein and GAGA factor on regulatory elements responsible for the maintenance of homeotic gene expression
    H Strutt
    ZMBH, University of Heidelberg, Germany
    EMBO J 16:3621-32. 1997
    ..We propose that GAGA factor binds constitutively to regulatory elements in the bithorax complex, which function both as PREs and as trithorax group response elements...
  58. ncbi Chromosomal homologies between Drosophila lebanonensis and D. melanogaster determined by in situ hybridization
    M Papaceit
    Departament de Genetica, Facultat de Biologia, Universitat de Barcelona, Spain
    Chromosoma 102:361-8. 1993
    ..Six probes correspond to known genes from D. melanogaster (RpII215, H3-H4, MHC, hsp28/23, hsp83, hsp70), four probes are clones isolated from a D...
  59. pmc Four heat shock proteins of Drosophila melanogaster coded within a 12-kilobase region in chromosome subdivision 67B
    V Corces
    Proc Natl Acad Sci U S A 77:5390-3. 1980
    ..Including the present results, the genes for all seven major heat shock proteins of D. melanogaster are now cloned are found to exhibit a variety of patterns of organization at the five loci they occupy...
  60. ncbi ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor
    T Tsukiyama
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    Nature 367:525-32. 1994
    ..ATP hydrolysis facilitates this process, suggesting that an energy-dependent pathway is involved in chromatin remodelling...
  61. ncbi Suppression of heat-shock protein synthesis by short-chain fatty acids and alcohols
    R J Munks
    Department of Anatomy, University of Birmingham Medical School, UK
    Biochim Biophys Acta 1223:23-8. 1994
    ..Suppression was accompanied by severely reduced levels of both hsp70 mRNA and active heat-shock factor (HSF). We conclude that the inhibitors act by suppressing the initiation of transcription of heat-shock genes...
  62. ncbi Polytene chromosomes show normal gene activity but some mRNAs are abnormally accumulated in the pseudonurse cell nuclei of Drosophila melanogaster otu mutants
    T I Heino
    Department of Biosciences, Division of Genetics, P O Box 56, FIN 00014 University of Helsinki, Finland
    Chromosoma 104:44-55. 1995
    ..We suggest that the otu mRNA remains partly attached to the polytene chromosome template after transcription and discuss the effects of this phenomenon on polytenisation of the PNC chromosomes...
  63. ncbi Short transcripts of the ternary complex provide insight into RNA polymerase II elongational pausing
    E B Rasmussen
    Section of Biochemistry Molecular and Cell Biology, Cornell University, Ithaca New York, 14853, USA
    J Mol Biol 252:522-35. 1995
    ..These included two of the small heat shock genes (hsp26 and hsp27) and two metabolic genes (Gapdh-1 and Gapdh-2), where paused polymerases were thought to be present, ..
  64. pmc Identification and characterization of a Drosophila nuclear receptor with the ability to inhibit the ecdysone response
    A C Zelhof
    Department of Biology, University of California, San Diego, La Jolla 92093, USA
    Proc Natl Acad Sci U S A 92:10477-81. 1995
    ..XR78E/F has its highest expression in third-instar larvae and prepupae. These experiments suggest that XR78E/F may play a regulatory role in the transcriptional cascade triggered by the hormone ecdysone in Drosophila...
  65. pmc Primary sequence of the 5' flanking regions of the Drosophila heat shock genes in chromosome subdivision 67B
    T D Ingolia
    Nucleic Acids Res 9:1627-42. 1981
    ..In addition, each of the four small genes contains the sequence ACTTTNA, 195 +/- 12 bp from the proposed site of initiation of transcription...
  66. ncbi Developmentally regulated transcription from Drosophila melanogaster chromosomal site 67B
    K Sirotkin
    Dev Biol 89:196-210. 1982
  67. pmc Four small Drosophila heat shock proteins are related to each other and to mammalian alpha-crystallin
    T D Ingolia
    Proc Natl Acad Sci U S A 79:2360-4. 1982
    ..The possible functional significance of this structural similarity is discussed...
  68. ncbi Accumulation of a specific subset of D. melanogaster heat shock mRNAs in normal development without heat shock
    J L Zimmerman
    Cell 32:1161-70. 1983
    ..melanogaster, messenger RNAs for three of the seven heat shock proteins (hsp83, hsp28 and hsp26) accumulate in adult ovaries and are abundant in embryos until blastoderm...
  69. ncbi Two protein-binding sites in chromatin implicated in the activation of heat-shock genes
    C Wu
    Nature 309:229-34. 1984
    ..The pattern of resistance before and after induction of gene expression suggests that heat-shock genes are activated by the sequential binding of at least two protein factors...
  70. ncbi Analysis of the resistance to heat and hydrogen peroxide stresses in COS cells transiently expressing wild type or deletion mutants of the Drosophila 27-kDa heat-shock protein
    P Mehlen
    Molecular and Cellular Genetics, CNRS UMR 106, Claude Bernard University, Lyon, France
    Eur J Biochem 215:277-84. 1993
    The Drosophila melanogaster small heat-shock protein, hsp27 (Dhsp27) belongs to a family of polypeptides which shares a sequence related to alpha-crystallin and which protect cell against heat shock...
  71. ncbi Patterns of puffing activity in the salivary gland chromosomes of Drosophila. V. Responses to environmental treatments
    M Ashburner
    Chromosoma 31:356-76. 1970
  72. ncbi Heat shock gene expression and development. I. An overview of fungal, plant, and poikilothermic animal developmental systems
    J J Heikkila
    Department of Biology, University of Waterloo, Ontario, Canada
    Dev Genet 14:1-5. 1993
  73. ncbi Heat shock proteins in three related Drosophila species belonging to the obscura group
    M D Molto
    Department of Genetics, Faculty of Biological Sciences, University of Valencia, Spain
    Experientia 49:54-6. 1993
    ..By means of in situ hybridization using D. melanogaster probes for the small hsp genes, it was inferred that the small hsp genes of the obscura group species are clustered at the 27A locus in all three species...
  74. pmc Functional redundancy in the tissue-specific enhancer of the Drosophila Sgs-4 gene
    T A Jongens
    Department of Molecular Biology, University of California, Berkeley 94720
    EMBO J 7:2559-67. 1988
    ..For at least this regulatory activity the enhancer is internally redundant. Any pairwise combination of the three elements is sufficient to direct salivary gland expression, although none of the three can act alone...
  75. ncbi The Drosophila hsp70 message is rapidly degraded at normal temperatures and stabilized by heat shock
    R Petersen
    Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637
    Gene 72:161-8. 1988
    ..Apparently, the hsp70 message is inherently unstable. During heat-shock, degradation of the message is suspended; during recovery degradation is restored...
  76. pmc Locus 67B of Drosophila melanogaster contains seven, not four, closely related heat shock genes
    A Ayme
    EMBO J 4:2949-54. 1985
    ..We conclude that genes 1, 2 and 3 are also heat shock genes. Therefore, the locus 67B contains seven, not four, small heat shock genes...
  77. pmc Human hsp27, Drosophila hsp27 and human alphaB-crystallin expression-mediated increase in glutathione is essential for the protective activity of these proteins against TNFalpha-induced cell death
    P Mehlen
    Laboratoire du Stress Cellulaire, Centre de Génétique Moléculaire etCellulaire, CNRS UMR 5534, France
    EMBO J 15:2695-706. 1996
    ..Recently, we have shown that the expression of shsp from different species, such as human hsp27, Drosophila hsp27 or human alphaB-crystallin protected murine L929 cells against cell death induced by tumor ..
  78. ncbi Nucleotide sequence analysis of the Drosophila small heat shock gene cluster at locus 67B
    R Southgate
    J Mol Biol 165:35-57. 1983
    ..Sequence analysis with respect to transcription initiation and termination and possible regulatory signals is discussed together with some structural predictions for the four proteins...
  79. ncbi Transcriptional regulation in Drosophila during heat shock: a nuclear run-on analysis
    J Vazquez
    Departement de Biologie Moleculaire, Universite de Geneve, Switzerland
    Chromosoma 102:233-48. 1993
    ..2 polymerases per gene). Engaged but paused RNA polymerase molecules were found at the hsp70 and hsp26 genes, but not at the other heat shock genes...
  80. pmc Rapid changes in Drosophila transcription after an instantaneous heat shock
    T O'BRIEN
    Section of Genetics and Development, Cornell University, Ithaca, New York 14853
    Mol Cell Biol 13:3456-63. 1993
    ..A similar rapid induction was found for the small heat shock genes (hsp22, hsp23, hsp26, and hsp27)...
  81. ncbi Characterization of an EcR/USP heterodimer target site that mediates ecdysone responsiveness of the Drosophila Lsp-2 gene
    C Antoniewski
    Institut Jacques Monod, Centre National de la Recherche Scientifique and Université de Paris 7, France
    Mol Gen Genet 249:545-56. 1995
    ..affinity of the Lsp-2 EcRE for the ecdysone receptor complex is comparable to that of the canonical EcRE of the hsp27 gene and is at least 4-fold greater than that of Fbp1, another fat body-specific Drosophila gene...
  82. ncbi The induction of gene activity in drosophilia by heat shock
    M Ashburner
    Cell 17:241-54. 1979
  83. ncbi A Drosophila heat shock gene from locus 67B is expressed during embryogenesis and pupation
    D Pauli
    Department of Molecular Biology, University of Geneva, Switzerland
    J Mol Biol 198:235-40. 1987
    ..In gene 3, this homology is restricted to the first 50 residues along the conserved 83 amino acid stretch. Two heat shock regulatory elements are localized upstream from the gene...
  84. ncbi Stage-specific localization of the small heat shock protein Hsp27 during oogenesis in Drosophila melanogaster
    R Marin
    Centre de Recherches du CHUL and Laboratoire de génétique cellulaire et développementale, RSVS, Pavillon C E Marchand, Universite Laval, Ste Foy, Quebec, Canada G1K 7P4
    Chromosoma 105:142-9. 1996
    The developmental and heat shock-induced expression of the small heat shock protein Hsp27 was investigated by confocal microscopy of whole-mount immunostained preparations of ovarioles during oogenesis in Drosophila melanogaster...
  85. ncbi A DNA segment isolated from chromosomal site 67B in D. melanogaster contains four closely linked heat-shock genes
    R Voellmy
    Cell 23:261-70. 1981
    ..0-4.7 kb. Three of the genes were found to exhibit alternating polarities. Thus in spite of their close linkage, the four heat-induced genes are most likely organized in individual transcription units...
  86. ncbi Identification of ecdysone response elements by analysis of the Drosophila Eip28/29 gene
    L Cherbas
    Institute for Molecular and Cellular Biology, Indiana University, Bloomington 47405
    Genes Dev 5:120-31. 1991
    ..We have also demonstrated that an EcRE-containing region from hsp27 contains a receptor-binding site...
  87. ncbi Genetic variation in the expression of the six hsp genes in the presence of heat shock in Drosophila melanogaster
    Y Otsuka
    Division of Medical Zoology, Oita Medical University, Japan
    Genes Genet Syst 72:19-24. 1997
    Genetic variation in total mRNA level of the six hsp genes (hsp22, hsp23, hsp26, hsp27, hsp70 and hsp82) in the presence of heat shock was investigated by using seventy-four second- and seventy third-chromosome lines of Drosophila ..