Gene Symbol: His3:CG31613
Description: histone H3
Alias: CG31613, Dmel\CG31613, His3, histone H3, CG31613-PA, His3:CG31613-PA
Species: fruit fly
Products:     His3:CG31613

Top Publications

  1. Lagarou A, Mohd Sarip A, Moshkin Y, Chalkley G, Bezstarosti K, Demmers J, et al. dKDM2 couples histone H2A ubiquitylation to histone H3 demethylation during Polycomb group silencing. Genes Dev. 2008;22:2799-810 pubmed publisher
    ..of histone crosstalk during gene silencing, in which histone H2A monoubiquitylation is coupled to the removal of histone H3 Lys 36 dimethylation (H3K36me2)...
  2. Fiedler M, Sánchez Barrena M, Nekrasov M, Mieszczanek J, Rybin V, Muller J, et al. Decoding of methylated histone H3 tail by the Pygo-BCL9 Wnt signaling complex. Mol Cell. 2008;30:507-18 pubmed publisher
    ..Pygo PHD fingers associate with their cognate HD1 domains from BCL9/Legless to bind specifically to the histone H3 tail methylated at lysine 4 (H3K4me)...
  3. Grimm C, de Ayala Alonso A, Rybin V, Steuerwald U, Ly Hartig N, Fischle W, et al. Structural and functional analyses of methyl-lysine binding by the malignant brain tumour repeat protein Sex comb on midleg. EMBO Rep. 2007;8:1031-7 pubmed
    ..Functional analyses in Drosophila show that the MBT domain of Scm and its methyl-lysine-binding activity are required for repression of Hox genes. ..
  4. Rudolph T, Yonezawa M, Lein S, Heidrich K, Kubicek S, Schafer C, et al. Heterochromatin formation in Drosophila is initiated through active removal of H3K4 methylation by the LSD1 homolog SU(VAR)3-3. Mol Cell. 2007;26:103-15 pubmed
  5. Lee N, Zhang J, Klose R, Erdjument Bromage H, Tempst P, Jones R, et al. The trithorax-group protein Lid is a histone H3 trimethyl-Lys4 demethylase. Nat Struct Mol Biol. 2007;14:341-3 pubmed
    ..However, no demethylase specific to histone H3 trimethyl-Lys4 (H3K4me3) has been identified...
  6. Wysocka J, Swigut T, Xiao H, Milne T, Kwon S, Landry J, et al. A PHD finger of NURF couples histone H3 lysine 4 trimethylation with chromatin remodelling. Nature. 2006;442:86-90 pubmed
    ..Trimethylation of histone H3 lysine 4 (H3K4me3) marks transcription start sites of virtually all active genes...
  7. Labrador M, Corces V. Phosphorylation of histone H3 during transcriptional activation depends on promoter structure. Genes Dev. 2003;17:43-8 pubmed
    ..b>Histone H3 associated with the DNA of Gal4-induced transcribing transgenes driven by the Drosophila Hsp70 promoter is ..
  8. Ito T, Bulger M, Kobayashi R, Kadonaga J. Drosophila NAP-1 is a core histone chaperone that functions in ATP-facilitated assembly of regularly spaced nucleosomal arrays. Mol Cell Biol. 1996;16:3112-24 pubmed
    ..Thus, NAP-1 appears to be one component of a multifactor chromatin assembly machinery that mediates the ATP-facilitated assembly of regularly spaced nucleosomal arrays. ..
  9. Mohn F, Sienski G, Handler D, Brennecke J. The rhino-deadlock-cutoff complex licenses noncanonical transcription of dual-strand piRNA clusters in Drosophila. Cell. 2014;157:1364-79 pubmed publisher
    ..Likely, this process involves 5' end protection of nascent RNAs and suppression of transcription termination. Our data provide key insight into the regulation and evolution of piRNA clusters...

More Information


  1. Chen C, Dechassa M, Bettini E, Ledoux M, Belisario C, Heun P, et al. CAL1 is the Drosophila CENP-A assembly factor. J Cell Biol. 2014;204:313-29 pubmed publisher
    Centromeres are specified epigenetically by the incorporation of the histone H3 variant CENP-A...
  2. Miller T, Mieszczanek J, S nchez Barrena M, Rutherford T, Fiedler M, Bienz M. Evolutionary adaptation of the fly Pygo PHD finger toward recognizing histone H3 tail methylated at arginine 2. Structure. 2013;21:2208-20 pubmed publisher
    ..Their PHD fingers bind histone H3 tail methylated at lysine 4, and to the HD1 domain of their Legless/BCL9 cofactors, linking Pygo to Armadillo/?-..
  3. Bodai L, Zsindely N, Gáspár R, Kristó I, Komonyi O, Boros I. Ecdysone induced gene expression is associated with acetylation of histone H3 lysine 23 in Drosophila melanogaster. PLoS ONE. 2012;7:e40565 pubmed publisher
    ..We found that acetylation of histone H3 lysine 23 is localized to promoters and correlates with endogenous ecdysone induced gene activation...
  4. Tie F, Stratton C, Kurzhals R, Harte P. The N terminus of Drosophila ESC binds directly to histone H3 and is required for E(Z)-dependent trimethylation of H3 lysine 27. Mol Cell Biol. 2007;27:2014-26 pubmed
    ..The 600-kDa E(Z)/ESC complex, also known as Polycomb repressive complex 2 (PRC2), specifically methylates histone H3 lysine 27 (H3 K27) through the intrinsic histone methyltransferase (HMTase) activity of the E(Z) SET domain...
  5. Jacobs S, Taverna S, Zhang Y, Briggs S, Li J, Eissenberg J, et al. Specificity of the HP1 chromo domain for the methylated N-terminus of histone H3. EMBO J. 2001;20:5232-41 pubmed
    ..heterochromatin-associated protein-1 (HP1) recognizes a 'histone code' involving methylated Lys9 (methyl-K9) in histone H3. Using in situ immunofluorescence, we demonstrate that methyl-K9 H3 and HP1 co-localize to the heterochromatic ..
  6. Schotta G, Ebert A, Krauss V, Fischer A, Hoffmann J, Rea S, et al. Central role of Drosophila SU(VAR)3-9 in histone H3-K9 methylation and heterochromatic gene silencing. EMBO J. 2002;21:1121-31 pubmed
    ..pombe homologues, Su(var) 3-9 encodes a histone methyltransferase (HMTase), which selectively methylates histone H3 at lysine 9 (H3-K9)...
  7. Byrd K, Shearn A. ASH1, a Drosophila trithorax group protein, is required for methylation of lysine 4 residues on histone H3. Proc Natl Acad Sci U S A. 2003;100:11535-40 pubmed
    ..As examples, methylation of lysine 9 residues of histone H3 (H3) (H3-K9) is believed to repress transcription by compacting chromatin, whereas methylation of lysine 4 ..
  8. Lin C, Li B, Swanson S, Zhang Y, Florens L, Washburn M, et al. Heterochromatin protein 1a stimulates histone H3 lysine 36 demethylation by the Drosophila KDM4A demethylase. Mol Cell. 2008;32:696-706 pubmed publisher
    ..Collectively, these results suggest a function of HP1a in transcription facilitating H3K36 demethylation at transcribed and/or heterochromatin regions. ..
  9. Kwon S, Florens L, Swanson S, Washburn M, Abmayr S, Workman J. Heterochromatin protein 1 (HP1) connects the FACT histone chaperone complex to the phosphorylated CTD of RNA polymerase II. Genes Dev. 2010;24:2133-45 pubmed publisher
    ..The absence of HP1c partially impairs the recruitment of FACT into heat-shock loci and causes a defect in heat-shock gene expression. Thus, HP1c functions to recruit the FACT complex to RNA polymerase II. ..
  10. Jacobs S, Khorasanizadeh S. Structure of HP1 chromodomain bound to a lysine 9-methylated histone H3 tail. Science. 2002;295:2080-3 pubmed
    ..structural, energetic, and mutational analyses of the complex between the Drosophila HP1 chromodomain and the histone H3 tail with a methyllysine at residue 9, a modification associated with epigenetic silencing...
  11. Morettini S, Tribus M, Zeilner A, Sebald J, Campo Fernandez B, Scheran G, et al. The chromodomains of CHD1 are critical for enzymatic activity but less important for chromatin localization. Nucleic Acids Res. 2011;39:3103-15 pubmed publisher
    ..A key feature of CHD1 is the presence of two chromodomains, which can bind to histone H3 methylated at lysine 4 and thus might serve to recruit and/or maintain CHD1 at the chromatin...
  12. Tie F, Banerjee R, Saiakhova A, Howard B, Monteith K, Scacheri P, et al. Trithorax monomethylates histone H3K4 and interacts directly with CBP to promote H3K27 acetylation and antagonize Polycomb silencing. Development. 2014;141:1129-39 pubmed publisher
    ..These data indicate that the catalytic activities of TRX and CBP are physically coupled and suggest that both activities play roles in antagonizing Polycomb silencing, stimulating enhancer activity and cellular memory...
  13. Nowak S, Pai C, Corces V. Protein phosphatase 2A activity affects histone H3 phosphorylation and transcription in Drosophila melanogaster. Mol Cell Biol. 2003;23:6129-38 pubmed
    ..heat shock genes during the heat shock response in Drosophila has been intimately linked to phosphorylation of histone H3 at serine 10, whereas repression of non-heat-shock genes correlates with dephosphorylation of histone H3...
  14. Wang L, Brown J, Cao R, Zhang Y, Kassis J, Jones R. Hierarchical recruitment of polycomb group silencing complexes. Mol Cell. 2004;14:637-46 pubmed
    ..DNA binding Pho and Phol proteins directly recruit E(z)-containing complexes, which in turn methylate histone H3 at lysine 27 (H3mK27). This provides a tag that facilitates binding by a Pc-containing complex...
  15. Eskeland R, Czermin B, Boeke J, Bonaldi T, Regula J, Imhof A. The N-terminus of Drosophila SU(VAR)3-9 mediates dimerization and regulates its methyltransferase activity. Biochemistry. 2004;43:3740-9 pubmed
    ..Full-length dSU(VAR)3-9 specifically methylates lysine 9 within histone H3 on peptides, on intact histones, and, to a lesser extent, on nucleosomes...
  16. Boulanger M, Miranda T, Clarke S, Di Fruscio M, Suter B, Lasko P, et al. Characterization of the Drosophila protein arginine methyltransferases DART1 and DART4. Biochem J. 2004;379:283-9 pubmed
    ..The presence of PRMTs in D. melanogaster suggest that flies are a suitable genetic system to study arginine methylation. ..
  17. Carrier F, Georgel P, Pourquier P, Blake M, Kontny H, Antinore M, et al. Gadd45, a p53-responsive stress protein, modifies DNA accessibility on damaged chromatin. Mol Cell Biol. 1999;19:1673-85 pubmed
    ..Both histone acetylation and UV radiation are thought to destabilize the nucleosomal structure. Hence, these results imply that Gadd45 can recognize an altered chromatin state and modulate DNA accessibility to cellular proteins. ..
  18. Pérez Montero S, Carbonell A, Morán T, Vaquero A, Azorín F. The embryonic linker histone H1 variant of Drosophila, dBigH1, regulates zygotic genome activation. Dev Cell. 2013;26:578-90 pubmed publisher
    ..Mutant embryos die at cellularization, showing increased levels of active RNApol II and zygotic transcripts, along with DNA damage and mitotic defects. These results show an essential function of dBigH1 in ZGA regulation. ..
  19. Huang F, Saraf A, Florens L, Kusch T, Swanson S, Szerszen L, et al. The Enok acetyltransferase complex interacts with Elg1 and negatively regulates PCNA unloading to promote the G1/S transition. Genes Dev. 2016;30:1198-210 pubmed publisher
    ..Taken together, our study provides novel insights into the roles of KAT6 HATs in cell cycle regulation through modulating PCNA levels on chromatin. ..
  20. Li L, Greer C, Eisenman R, Secombe J. Essential functions of the histone demethylase lid. PLoS Genet. 2010;6:e1001221 pubmed publisher
    ..described member of the JmjC domain class of histone demethylases that specifically targets trimethylated histone H3 lysine 4 (H3K4me3)...
  21. Zhou B, Feng H, Ghirlando R, Li S, Schwieters C, Bai Y. A Small Number of Residues Can Determine if Linker Histones Are Bound On or Off Dyad in the Chromatosome. J Mol Biol. 2016;428:3948-3959 pubmed publisher
    ..Taken together, our results suggest that a small number of residues in the globular domain of a linker histone can control its binding location on the nucleosome and higher-order chromatin structure. ..
  22. Klymenko T, Papp B, Fischle W, Kocher T, Schelder M, Fritsch C, et al. A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysine-binding activities. Genes Dev. 2006;20:1110-22 pubmed
    ..We propose that PRE-tethered PhoRC selectively interacts with methylated histones in the chromatin flanking PREs to maintain a Polycomb-repressed chromatin state. ..
  23. Czermin B, Melfi R, McCabe D, Seitz V, Imhof A, Pirrotta V. Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites. Cell. 2002;111:185-96 pubmed
    ..We show that this complex contains a methyltransferase activity that methylates lysine 9 and lysine 27 of histone H3, but the activity is lost when the E(Z) SET domain is mutated...
  24. Karam C, Kellner W, Takenaka N, Clemmons A, Corces V. 14-3-3 mediates histone cross-talk during transcription elongation in Drosophila. PLoS Genet. 2010;6:e1000975 pubmed publisher
    ..Studies in Drosophila have shown that the phosphorylation of histone H3 at Ser10 (H3S10ph) by JIL-1 is required specifically during early transcription elongation...
  25. Yu B, Cassani M, Wang M, Liu M, Ma J, Li G, et al. Structural insights into Rhino-mediated germline piRNA cluster formation. Cell Res. 2015;25:525-8 pubmed publisher
  26. Bulger M, Ito T, Kamakaka R, Kadonaga J. Assembly of regularly spaced nucleosome arrays by Drosophila chromatin assembly factor 1 and a 56-kDa histone-binding protein. Proc Natl Acad Sci U S A. 1995;92:11726-30 pubmed
    ..The dCAF-4 activity copurified with a 56-kDa core-histone-binding protein that was purified to > 90% homogeneity. ..
  27. Muller J, Hart C, Francis N, Vargas M, Sengupta A, Wild B, et al. Histone methyltransferase activity of a Drosophila Polycomb group repressor complex. Cell. 2002;111:197-208 pubmed
    ..A recombinant complex reconstituted from these four subunits methylates lysine-27 of histone H3. Mutations in the E(Z) SET domain disrupt methyltransferase activity in vitro and HOX gene repression in vivo...
  28. Patel A, Vought V, Swatkoski S, Viggiano S, Howard B, Dharmarajan V, et al. Automethylation activities within the mixed lineage leukemia-1 (MLL1) core complex reveal evidence supporting a "two-active site" model for multiple histone H3 lysine 4 methylation. J Biol Chem. 2014;289:868-84 pubmed publisher
    The mixed lineage leukemia-1 (MLL1) core complex predominantly catalyzes mono- and dimethylation of histone H3 at lysine 4 (H3K4) and is frequently altered in aggressive acute leukemias...
  29. Tyler J, Adams C, Chen S, Kobayashi R, Kamakaka R, Kadonaga J. The RCAF complex mediates chromatin assembly during DNA replication and repair. Nature. 1999;402:555-60 pubmed
  30. Cao R, Wang L, Wang H, Xia L, Erdjument Bromage H, Tempst P, et al. Role of histone H3 lysine 27 methylation in Polycomb-group silencing. Science. 2002;298:1039-43 pubmed
    ..We demonstrate that the complex specifically methylates nucleosomal histone H3 at lysine 27 (H3-K27)...
  31. Doyen C, Moshkin Y, Chalkley G, Bezstarosti K, Demmers J, Rathke C, et al. Subunits of the histone chaperone CAF1 also mediate assembly of protamine-based chromatin. Cell Rep. 2013;4:59-65 pubmed publisher
    ..These results show that CAF1 subunits mediate the assembly of two fundamentally different forms of chromatin. ..
  32. Smith E, Pannuti A, Gu W, Steurnagel A, Cook R, Allis C, et al. The drosophila MSL complex acetylates histone H4 at lysine 16, a chromatin modification linked to dosage compensation. Mol Cell Biol. 2000;20:312-8 pubmed
    ..These results demonstrate that the MSL complex is responsible for the specific chromatin modification characteristic of the X chromosome in Drosophila males. ..
  33. Emelyanov A, Rabbani J, Mehta M, Vershilova E, Keogh M, Fyodorov D. Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization. Genes Dev. 2014;28:2027-40 pubmed publisher
  34. Kusch T, Mei A, Nguyen C. Histone H3 lysine 4 trimethylation regulates cotranscriptional H2A variant exchange by Tip60 complexes to maximize gene expression. Proc Natl Acad Sci U S A. 2014;111:4850-5 pubmed publisher
    b>Histone H3 lysine 4 trimethylation (H3K4me3) and the acetylated H2A variant, H2A.Z/v (H2Avac), are enriched at promoters of highly transcribed loci including the stress response genes...
  35. Yang S, Baxter E, Van Doren M. Phf7 controls male sex determination in the Drosophila germline. Dev Cell. 2012;22:1041-51 pubmed publisher
    ..PHF7 associates with chromatin, and both the human and fly proteins bind histone H3 N-terminal tails with a preference for dimethyl lysine 4 (H3K4me2)...
  36. Tie F, Banerjee R, Conrad P, Scacheri P, Harte P. Histone demethylase UTX and chromatin remodeler BRM bind directly to CBP and modulate acetylation of histone H3 lysine 27. Mol Cell Biol. 2012;32:2323-34 pubmed publisher
    ..We previously showed that the Drosophila melanogaster acetyltransferase CREB-binding protein (CBP) acetylates histone H3 lysine 27 (H3K27ac), thereby directly blocking its trimethylation (H3K27me3) by Polycomb repressive complex 2 (..
  37. Eisert R, Waters M. Tuning HP1? chromodomain selectivity for di- and trimethyllysine. Chembiochem. 2011;12:2786-90 pubmed publisher
    ..Moreover, the information from this study may help guide inhibitor development for this class of proteins. ..
  38. Nowak A, Alfieri C, Stirnimann C, Rybin V, Baudin F, Ly Hartig N, et al. Chromatin-modifying complex component Nurf55/p55 associates with histones H3 and H4 and polycomb repressive complex 2 subunit Su(z)12 through partially overlapping binding sites. J Biol Chem. 2011;286:23388-96 pubmed publisher
    ..1, we analyzed interactions of Nurf55 (Nurf55 or p55 in fly and RbAp48/46 in human) with the N-terminal tail of histone H3, the first helix of histone H4, and an N-terminal fragment of the PRC2 subunit Su(z)12 using isothermal ..
  39. Tanaka Y, Katagiri Z, Kawahashi K, Kioussis D, Kitajima S. Trithorax-group protein ASH1 methylates histone H3 lysine 36. Gene. 2007;397:161-8 pubmed
    ..Drosophila ASH1 genetically interacts with trithorax and has been reported to methylate histone H3 lysine 4 (K4) as well as H3 K9 and H4 K20...
  40. Lu X, Wontakal S, Kavi H, Kim B, Guzzardo P, Emelyanov A, et al. Drosophila H1 regulates the genetic activity of heterochromatin by recruitment of Su(var)3-9. Science. 2013;340:78-81 pubmed publisher
    ..H1-mediated silencing occurs in combination with the heterochromatin-specific histone H3 lysine 9 methyltransferase Su(var)3-9...
  41. Rai A, Vargas M, Wang L, Andersen E, Miller E, Simon J. Elements of the polycomb repressor SU(Z)12 needed for histone H3-K27 methylation, the interface with E(Z), and in vivo function. Mol Cell Biol. 2013;33:4844-56 pubmed publisher
    ..PRC2 methylates histone H3 on lysine-27 and is conserved from plants to flies to humans...
  42. Yuan W, Wu T, Fu H, Dai C, Wu H, Liu N, et al. Dense chromatin activates Polycomb repressive complex 2 to regulate H3 lysine 27 methylation. Science. 2012;337:971-5 pubmed publisher
    Polycomb repressive complex 2 (PRC2)-mediated histone H3 lysine 27 (H3K27) methylation is vital for Polycomb gene silencing, a classic epigenetic phenomenon that maintains transcriptional silencing throughout cell divisions...
  43. Ito T, Tyler J, Bulger M, Kobayashi R, Kadonaga J. ATP-facilitated chromatin assembly with a nucleoplasmin-like protein from Drosophila melanogaster. J Biol Chem. 1996;271:25041-8 pubmed
    ..These and other data collectively suggest that dNLP may participate in parallel with other histone-binding proteins such as dNAP-1 in the assembly of chromatin. ..
  44. Le Thomas A, Stuwe E, Li S, Du J, Marinov G, Rozhkov N, et al. Transgenerationally inherited piRNAs trigger piRNA biogenesis by changing the chromatin of piRNA clusters and inducing precursor processing. Genes Dev. 2014;28:1667-80 pubmed publisher
  45. Klinker H, Haas C, Harrer N, Becker P, Mueller Planitz F. Rapid purification of recombinant histones. PLoS ONE. 2014;9:e104029 pubmed publisher
    ..We expect that the RHP protocol can be readily applied to the purification of canonical histones from other species as well as the numerous histone variants. ..
  46. Schmitges F, Prusty A, Faty M, Stützer A, Lingaraju G, Aiwazian J, et al. Histone methylation by PRC2 is inhibited by active chromatin marks. Mol Cell. 2011;42:330-41 pubmed publisher
    The Polycomb repressive complex 2 (PRC2) confers transcriptional repression through histone H3 lysine 27 trimethylation (H3K27me3)...
  47. Bao X, Cai W, Deng H, Zhang W, Krencik R, Girton J, et al. The COOH-terminal domain of the JIL-1 histone H3S10 kinase interacts with histone H3 and is required for correct targeting to chromatin. J Biol Chem. 2008;283:32741-50 pubmed publisher
    ..show that a small 53-amino acid region within the COOH-terminal domain can interact with the tail region of histone H3, suggesting that this interaction is necessary for the correct chromatin targeting of the JIL-1 kinase...
  48. Eberharter A, Vetter I, Ferreira R, Becker P. ACF1 improves the effectiveness of nucleosome mobilization by ISWI through PHD-histone contacts. EMBO J. 2004;23:4029-39 pubmed
    ..Connecting ACF to histones may be prerequisite for efficient conversion of ATP-dependent conformational changes of ISWI into translocation of DNA relative to the histones during nucleosome mobilization. ..