His3.3A

Summary

Gene Symbol: His3.3A
Description: Histone H3.3A
Alias: CG5825, Dmel\CG5825, H3.3, H3.3A, His-3.3A, His3.3, PH3, dH3.3A, histone H3.3A, CG5825-PA, CG5825-PC, CG5825-PD, H3L-like histone, His3.3A-PA, His3.3A-PC, His3.3A-PD, histone , histone H3.3, histone H3.3
Species: fruit fly
Products:     His3.3A

Top Publications

  1. Akhmanova A, Bindels P, Xu J, Miedema K, Kremer H, Hennig W. Structure and expression of histone H3.3 genes in Drosophila melanogaster and Drosophila hydei. Genome. 1995;38:586-600 pubmed
    We demonstrate that in Drosophila melanogaster the histone H3.3 replacement variant is encoded by two genes, H3.3A and H3.3B. We have isolated cDNA clones for H3.3A and cDNA and genomic clones for H3.3B...
  2. Schwartz B, Ahmad K. Transcriptional activation triggers deposition and removal of the histone variant H3.3. Genes Dev. 2005;19:804-14 pubmed
    ..In contrast, we find that the Drosophila HSP70 genes rapidly lose histone H3 and acquire variant H3.3 histones as they are induced...
  3. Akhmanova A, Miedema K, Wang Y, van Bruggen M, Berden J, Moudrianakis E, et al. The localization of histone H3.3 in germ line chromatin of Drosophila males as established with a histone H3.3-specific antiserum. Chromosoma. 1997;106:335-47 pubmed
    A rabbit antiserum, specific for the histone H3.3 replacement variant, was raised with the aid of a histone H3.3-specific peptide. Immuno blot experiments demonstrated the specificity of this polyclonal antiserum...
  4. Deal R, Henikoff J, Henikoff S. Genome-wide kinetics of nucleosome turnover determined by metabolic labeling of histones. Science. 2010;328:1161-4 pubmed publisher
    ..underlie their opposing activities and challenging models for epigenetic inheritance that rely on stability of histone marks...
  5. Loppin B, Bonnefoy E, Anselme C, Laurencon A, Karr T, Couble P. The histone H3.3 chaperone HIRA is essential for chromatin assembly in the male pronucleus. Nature. 2005;437:1386-90 pubmed
    ..Here we show that ssm is a point mutation in the Hira gene, thus demonstrating that the histone chaperone protein HIRA is required for nucleosome assembly during sperm nucleus decondensation...
  6. Ahmad K, Henikoff S. Histone H3 variants specify modes of chromatin assembly. Proc Natl Acad Sci U S A. 2002;99 Suppl 4:16477-84 pubmed
    b>Histone variants have been known for 30 years, but their functions and the mechanism of their deposition are still largely unknown. Drosophila has three versions of histone H3. H3 packages the bulk genome, H3...
  7. Hödl M, Basler K. Transcription in the absence of histone H3.3. Curr Biol. 2009;19:1221-6 pubmed publisher
    Di- and trimethylation of histone H3 lysine 4 (H3K4me2 and H3K4me3) are hallmarks of chromatin at active genes. The major fraction of K4-methylated histone H3 is the variant H3 (termed H3.3 in Drosophila), which replaces canonical H3 (H3...
  8. McKittrick E, Gafken P, Ahmad K, Henikoff S. Histone H3.3 is enriched in covalent modifications associated with active chromatin. Proc Natl Acad Sci U S A. 2004;101:1525-30 pubmed
    Chromatin states can be distinguished by differential covalent modifications of histones or by utilization of histone variants...
  9. Ahmad K, Henikoff S. The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly. Mol Cell. 2002;9:1191-200 pubmed
    ..RI substitution of H3.3 provides a mechanism for the immediate activation of genes that are silenced by histone modification. Inheritance of newly deposited nucleosomes may then mark sites as active loci.

More Information

Publications54

  1. Bell O, Wirbelauer C, Hild M, Scharf A, Schwaiger M, MacAlpine D, et al. Localized H3K36 methylation states define histone H4K16 acetylation during transcriptional elongation in Drosophila. EMBO J. 2007;26:4974-84 pubmed
    ..Methylation of lysine 36 of histone H3 (H3K36me) resides promoter distal at transcribed regions in Saccharomyces cerevisiae and is thought to prevent ..
  2. Nakayama T, Nishioka K, Dong Y, Shimojima T, Hirose S. Drosophila GAGA factor directs histone H3.3 replacement that prevents the heterochromatin spreading. Genes Dev. 2007;21:552-61 pubmed
    ..Interestingly there is a dip of histone H3 Lys 9 methylation and a peak of H3 Lys 4 methylation at this site...
  3. Henikoff S, Henikoff J, Sakai A, Loeb G, Ahmad K. Genome-wide profiling of salt fractions maps physical properties of chromatin. Genome Res. 2009;19:460-9 pubmed publisher
    ..soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II...
  4. Mito Y, Henikoff J, Henikoff S. Genome-scale profiling of histone H3.3 replacement patterns. Nat Genet. 2005;37:1090-7 pubmed
    ..When chromatin assembly occurs at other times, the histone H3.3 variant replaces canonical H3...
  5. Sakai A, Schwartz B, Goldstein S, Ahmad K. Transcriptional and developmental functions of the H3.3 histone variant in Drosophila. Curr Biol. 2009;19:1816-20 pubmed publisher
    ..In most animals, replacement uses the conserved H3.3 histone variant, but the functions of this variant have not been defined. Using mutations for the two H3...
  6. Mito Y, Henikoff J, Henikoff S. Histone replacement marks the boundaries of cis-regulatory domains. Science. 2007;315:1408-11 pubmed
    ..We have examined chromatin at Drosophila homeotic gene clusters by measuring, at high resolution, levels of histone replacement and nucleosome occupancy...
  7. Konev A, Tribus M, Park S, Podhraski V, Lim C, Emelyanov A, et al. CHD1 motor protein is required for deposition of histone variant H3.3 into chromatin in vivo. Science. 2007;317:1087-90 pubmed
    ..H3.3 is an evolutionarily conserved histone variant and a key substrate for replication-independent chromatin assembly...
  8. Polo S, Almouzni G. Chromatin assembly: a basic recipe with various flavours. Curr Opin Genet Dev. 2006;16:104-11 pubmed
    Packaging of eukaryotic genomes into chromatin is a hierarchical mechanism, starting with histone deposition onto DNA to produce nucleosome arrays, which then further fold and ultimately form functional domains...
  9. Wirbelauer C, Bell O, Schübeler D. Variant histone H3.3 is deposited at sites of nucleosomal displacement throughout transcribed genes while active histone modifications show a promoter-proximal bias. Genes Dev. 2005;19:1761-6 pubmed
    ..active genes similar to polymerase, yet its distribution is very distinct from that of several euchromatic histone modifications, which are highly biased toward the 5' part of active genes...
  10. Schneiderman J, Sakai A, Goldstein S, Ahmad K. The XNP remodeler targets dynamic chromatin in Drosophila. Proc Natl Acad Sci U S A. 2009;106:14472-7 pubmed publisher
    ..Thus, the XNP focus appears to be a functional genetic element that can contribute to gene silencing throughout the nucleus. ..
  11. Dyson M, Thomson S, Inagaki M, Goto H, Arthur S, Nightingale K, et al. MAP kinase-mediated phosphorylation of distinct pools of histone H3 at S10 or S28 via mitogen- and stress-activated kinase 1/2. J Cell Sci. 2005;118:2247-59 pubmed
    ERK and p38 MAP kinases, acting through the downstream mitogen- and stress-activated kinase 1/2 (MSK1/2), elicit histone H3 phosphorylation on a subfraction of nucleosomes--including those at Fos and Jun--concomitant with gene induction...
  12. Yang S, Baxter E, Van Doren M. Phf7 controls male sex determination in the Drosophila germline. Dev Cell. 2012;22:1041-51 pubmed publisher
    ..PHF7 associates with chromatin, and both the human and fly proteins bind histone H3 N-terminal tails with a preference for dimethyl lysine 4 (H3K4me2)...
  13. Nakayama T, Shimojima T, Hirose S. The PBAP remodeling complex is required for histone H3.3 replacement at chromatin boundaries and for boundary functions. Development. 2012;139:4582-90 pubmed publisher
    Establishment and maintenance of epigenetic memories are essential for development. Replacement of canonical histone H3 by its variant H3.3 has been implicated in cellular memory...
  14. Henikoff S, Furuyama T, Ahmad K. Histone variants, nucleosome assembly and epigenetic inheritance. Trends Genet. 2004;20:320-6 pubmed
  15. Orsi G, Algazeery A, Meyer R, Capri M, Sapey Triomphe L, Horard B, et al. Drosophila Yemanuclein and HIRA cooperate for de novo assembly of H3.3-containing nucleosomes in the male pronucleus. PLoS Genet. 2013;9:e1003285 pubmed publisher
    ..This process exclusively uses the histone H3 variant H3...
  16. Tran V, Lim C, Xie J, Chen X. Asymmetric division of Drosophila male germline stem cell shows asymmetric histone distribution. Science. 2012;338:679-82 pubmed publisher
    ..The asymmetric histone distribution occurs in GSCs but not in symmetrically dividing progenitor cells...
  17. Kamakaka R, Biggins S. Histone variants: deviants?. Genes Dev. 2005;19:295-310 pubmed
    ..Here we discuss recent progress in understanding how histone variants lead to changes in chromatin structure and dynamics to carry out specific functions...
  18. Paranjape N, Calvi B. The Histone Variant H3.3 Is Enriched at Drosophila Amplicon Origins but Does Not Mark Them for Activation. G3 (Bethesda). 2016;6:1661-71 pubmed publisher
    ..analyses in Drosophila and other organisms have revealed a correlation between ORC binding sites and the histone variant H3.3. This correlation suggests that H3.3 may designate origin sites, but this idea has remained untested...
  19. Hennig W. Chromosomal proteins in the spermatogenesis of Drosophila. Chromosoma. 2003;111:489-94 pubmed
    ..During meiotic prophase cell cycle-regulated H3 histones appear to a large extent to be substituted by the histone H3.3 replacement variant protein, which is generally found associated with transcriptionally active chromatin...
  20. Hennig W, Weyrich A. Histone modifications in the male germ line of Drosophila. BMC Dev Biol. 2013;13:7 pubmed publisher
    ..A large proportion of the cell cycle-regulated histone H3.1 is replaced by H3...
  21. MacAlpine H, Gordân R, Powell S, Hartemink A, MacAlpine D. Drosophila ORC localizes to open chromatin and marks sites of cohesin complex loading. Genome Res. 2010;20:201-11 pubmed publisher
    ..ORC-associated sequences are enriched for the histone variant, H3.3, often at transcription start sites, and depleted for bulk nucleosomes...
  22. Su T, Campbell S, O Farrell P. Drosophila grapes/CHK1 mutants are defective in cyclin proteolysis and coordination of mitotic events. Curr Biol. 1999;9:919-22 pubmed
    ..The mitotic failure described previously for cycle 12 grp embryos might be a more severe form of the phenotypes that we describe in earlier embryos and we suggest that the underlying defect is reduced degradation of cyclin A. ..
  23. Sedkov Y, Cho E, Petruk S, Cherbas L, Smith S, Jones R, et al. Methylation at lysine 4 of histone H3 in ecdysone-dependent development of Drosophila. Nature. 2003;426:78-83 pubmed
    ..For example, p160 class coactivators associate with histone acetyltransferases and arginine histone methyltransferases...
  24. Whittaker A, Royzman I, Orr Weaver T. Drosophila double parked: a conserved, essential replication protein that colocalizes with the origin recognition complex and links DNA replication with mitosis and the down-regulation of S phase transcripts. Genes Dev. 2000;14:1765-76 pubmed
    ..This could be either because these events depend on progression of S phase beyond the point blocked in the dup mutants or because DUP is needed directly for these feedback mechanisms. ..
  25. Matsuo Y. Evolutionary change of codon usage for the histone gene family in Drosophila melanogaster and Drosophila hydei. Mol Phylogenet Evol. 2000;15:283-91 pubmed
    The nucleotide divergence in the protein-coding region for replication-dependent and replication-independent histone 3 and 4 genes of Drosophila melanogaster and Drosophila hydei occurred mostly at the synonymous site...
  26. Fromental Ramain C, Ramain P, Hamiche A. The Drosophila DAXX-Like Protein (DLP) Cooperates with ASF1 for H3.3 Deposition and Heterochromatin Formation. Mol Cell Biol. 2017;37: pubmed publisher
    b>Histone variants are nonallelic isoforms of canonical histones, and they are deposited, in contrast to canonical histones, in a replication-independent (RI) manner. RI deposition of H3.3, a histone variant from the H3...
  27. Li G, Zhou L. Genome-wide identification of chromatin transitional regions reveals diverse mechanisms defining the boundary of facultative heterochromatin. PLoS ONE. 2013;8:e67156 pubmed publisher
    ..However, boundary-associated insulator binding sites are distinctively flanked by nucleosome destabilizing sequences, which correlates with significantly decreased nucleosome density and increased binding of co-factors. ..
  28. Horard B, Loppin B. Histone storage and deposition in the early Drosophila embryo. Chromosoma. 2015;124:163-75 pubmed publisher
    ..In this article, we have reviewed our current understanding of the mechanisms involved in the production, storage, and deposition of histones in the fertilized egg and during the exponential amplification of cleavage nuclei. ..
  29. Chen W, Shih H, Liu K, Liu K, Shih Z, Chen L, et al. Intellectual disability-associated dBRWD3 regulates gene expression through inhibition of HIRA/YEM-mediated chromatin deposition of histone H3.3. EMBO Rep. 2015;16:528-38 pubmed publisher
    ..Replication-independent deposition of the histone H3...
  30. Sawatsubashi S, Murata T, Lim J, Fujiki R, Ito S, Suzuki E, et al. A histone chaperone, DEK, transcriptionally coactivates a nuclear receptor. Genes Dev. 2010;24:159-70 pubmed publisher
    ..purification and characterization of the complexes containing fly and human DEKs revealed that DEKs serve as histone chaperones via phosphorylation by forming complexes with casein kinase 2...
  31. Bonnefoy E, Orsi G, Couble P, Loppin B. The essential role of Drosophila HIRA for de novo assembly of paternal chromatin at fertilization. PLoS Genet. 2007;3:1991-2006 pubmed
    ..At fertilization, these non-histone proteins are removed from the decondensing sperm nucleus and replaced with maternally provided histones to form ..
  32. Pinnola A, Naumova N, Shah M, Tulin A. Nucleosomal core histones mediate dynamic regulation of poly(ADP-ribose) polymerase 1 protein binding to chromatin and induction of its enzymatic activity. J Biol Chem. 2007;282:32511-9 pubmed
    ..Finally, we have found that interaction with the N-terminal tail of the H4 histone triggers PARP1 enzymatic activity...
  33. Orian A. Chromatin profiling, DamID and the emerging landscape of gene expression. Curr Opin Genet Dev. 2006;16:157-64 pubmed
    ..The molecular picture that emerges from DamID and similar studies is that genomes integrate inputs from both genetic and epigenetic machineries to dynamically regulate gene expression. ..
  34. Feng R, Tang X, Becker A, Berger A, Ye J, Akhmanova A, et al. Regulation of the expression of histone H3.3 by differential polyadenylation. Genome. 2005;48:503-10 pubmed
    Previously we have shown that the 3' untranslated regions (UTRs) of the replacement histone genes H3.3.A and H3.3B of Drosophila melanogaster differ in their nucleotide sequences and have different polyadenylation sites...
  35. Guertin M, Lis J. Chromatin landscape dictates HSF binding to target DNA elements. PLoS Genet. 2010;6:e1001114 pubmed publisher
    ..generated during non-heat shock conditions, were used to show that inducibly bound HSE motifs are associated with histone acetylation, H3K4 trimethylation, RNA Polymerase II, and coactivators, compared to HSE motifs that remain HSF-free...
  36. Ito S, Fujiyama Nakamura S, Kimura S, Lim J, Kamoshida Y, Shiozaki Sato Y, et al. Epigenetic silencing of core histone genes by HERS in Drosophila. Mol Cell. 2012;45:494-504 pubmed publisher
    Cell cycle-dependent expression of canonical histone proteins enables newly synthesized DNA to be integrated into chromatin in replicating cells...
  37. Schneiderman J, Orsi G, Hughes K, Loppin B, Ahmad K. Nucleosome-depleted chromatin gaps recruit assembly factors for the H3.3 histone variant. Proc Natl Acad Sci U S A. 2012;109:19721-6 pubmed publisher
    ..Replication-independent nucleosome replacement using the H3.3 histone variant efficiently repackages these regions, but how histones are recruited to these sites is unknown...
  38. Gunjan A, Paik J, Verreault A. The emergence of regulated histone proteolysis. Curr Opin Genet Dev. 2006;16:112-8 pubmed
    ..In addition, cells need to ensure that histone variants are incorporated at the correct chromosomal location...
  39. Loppin B, Dubruille R, Horard B. The intimate genetics of Drosophila fertilization. Open Biol. 2015;5: pubmed publisher
  40. Debec A, Grammont M, Berson G, Dastugue B, Sullivan W, Couderc J. Toucan protein is essential for the assembly of syncytial mitotic spindles in Drosophila melanogaster. Genesis. 2001;31:167-75 pubmed
    ..These results strongly suggest that Toucan represents a factor essential for the assembly and the function of the syncytial mitotic spindles. ..
  41. de Wit E, Greil F, van Steensel B. High-resolution mapping reveals links of HP1 with active and inactive chromatin components. PLoS Genet. 2007;3:e38 pubmed
    ..HP1 target genes are also marked by the histone variant H3.3 and dimethylated histone 3 lysine 4 (H3K4me2), which are both typical of active chromatin...
  42. Sadasivam D, Huang D. Maintenance of Tissue Pluripotency by Epigenetic Factors Acting at Multiple Levels. PLoS Genet. 2016;12:e1005897 pubmed publisher
    ..the Antennapedia promoter is paused in eye-antenna discs, it cannot be induced by Trx without a reduction in histone variants or their chaperones, suggesting additional control by the nucleosomal architecture...
  43. Henikoff S. Visualizing gene expression: an unfolding story. Cell. 2004;116:633-4 pubmed
    ..They find that local decondensation of the template is accompanied by profound changes in chromatin composition. ..
  44. Braunschweig U, Hogan G, Pagie L, van Steensel B. Histone H1 binding is inhibited by histone variant H3.3. EMBO J. 2009;28:3635-45 pubmed publisher
    ..We generated a genome-wide high-resolution binding map for linker histone H1 in Drosophila cells, using DamID...
  45. Günesdogan U, Jäckle H, Herzig A. Histone supply regulates S phase timing and cell cycle progression. elife. 2014;3:e02443 pubmed publisher
    Eukaryotes package DNA into nucleosomes that contain a core of histone proteins. During DNA replication, nucleosomes are disrupted and re-assembled with newly synthesized histones and DNA...