His2Av

Summary

Gene Symbol: His2Av
Description: Histone H2A variant
Alias: 5499, CG5499, Dmel\CG5499, H2A, H2A.F/Z, H2A.X, H2A.Z, H2AV, H2AV_DROM, H2AX, H2AZ, H2Av, H2AvD, H2a.V, H2av, H2avD, HIS, HIS2AV, HIS2AVD, His, His2, His2AV, His2AvD, HisH2Av, Hist, Hist2av, gamma-H2Av, gamma-HIS2AV, gamma-His2Av, gammaH2Av, h2AvD, his, l(3)05146, l(3)810, l(3)97Dd, l(3)L1602, CG5499-PA, CG5499-PB, H2A variant, His2Av-PA, His2Av-PB, Histone-2A, Histone2Av, histone, histone 2A variant, histone H2A variant, histone H2Av, histone variant H2Av, histone2A, histone2AvD
Species: fruit fly

Top Publications

  1. ncbi Regions of variant histone His2AvD required for Drosophila development
    M J Clarkson
    The John Curtin School of Medical Research, The Australian National University, Canberra, Australian Capital Territory
    Nature 399:694-7. 1999
  2. ncbi Drosophila rasiRNA pathway mutations disrupt embryonic axis specification through activation of an ATR/Chk2 DNA damage response
    Carla Klattenhoff
    Program in Molecular Medicine and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Dev Cell 12:45-55. 2007
  3. ncbi A histone variant, H2AvD, is essential in Drosophila melanogaster
    A van Daal
    Department of Biology, Washington University, St Louis, Missouri 63130
    Mol Biol Cell 3:593-602. 1992
  4. ncbi DNA double-strand break-induced phosphorylation of Drosophila histone variant H2Av helps prevent radiation-induced apoptosis
    James P Madigan
    Wadsworth Center, New York State Department of Health, State University of New York, Albany, NY 12201 2002, USA
    Nucleic Acids Res 30:3698-705. 2002
  5. ncbi Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions
    Thomas Kusch
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Science 306:2084-7. 2004
  6. ncbi The degradation of two mitotic cyclins contributes to the timing of cytokinesis
    Arnaud Echard
    Department of Biochemistry and Biophysics, University of California, San Francisco, Genentech Hall, 600 16th Street, San Francisco, CA 94143, USA
    Curr Biol 13:373-83. 2003
  7. ncbi Relationship of DNA double-strand breaks to synapsis in Drosophila
    Janet K Jang
    Waksman Institute and Department of Genetics, Rutgers, The State University of New Jersey, 190 Frelinghuysen Rd, Piscataway, New Jersey 08854 8020, USA
    J Cell Sci 116:3069-77. 2003
  8. ncbi Temporal analysis of meiotic DNA double-strand break formation and repair in Drosophila females
    S Mehrotra
    Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America
    PLoS Genet 2:e200. 2006
  9. ncbi Onset of the DNA replication checkpoint in the early Drosophila embryo
    Justin Crest
    Department of Biology, University of Washington, Seattle, Washington 98195 1800, USA
    Genetics 175:567-84. 2007
  10. ncbi Both cyclin B levels and DNA-replication checkpoint control the early embryonic mitoses in Drosophila
    Jun Yuan Ji
    Department of Biology, University of Washington, Seattle, WA 98195 1800, USA
    Development 131:401-11. 2004

Research Grants

  1. Cell Growth and Radiation Survival in Drosophila
    Tin Tin Su; Fiscal Year: 2007
  2. Regulation of Radiation Induced Cell Death in Drosophila
    Tin Tin Su; Fiscal Year: 2010
  3. Perilipins and cellular triacyglycerol metabolism
    Dawn Brasaemle; Fiscal Year: 2007
  4. An in vivo Screen for Radiation Sensitizers
    Tin Tin Su; Fiscal Year: 2007
  5. Maintenance of blocks-based tools for functional genomic
    Steven Henikoff; Fiscal Year: 2006
  6. Cell Cycle Regulation by Checkpoints in Drosophila
    Tin Tin Su; Fiscal Year: 2006
  7. Mini spindles and microtubule function in Drosophila
    NANCY POKRYWKA; Fiscal Year: 2006
  8. Genome-wide profiling of histone variants in Drosophila and Caenorhabditis
    Steven Henikoff; Fiscal Year: 2007
  9. Epigenomic profiling of histone turnover kinetics in mammalian cells
    Steven Henikoff; Fiscal Year: 2010
  10. Perilipins and cellular triacyglycerol metabolism
    Dawn L Brasaemle; Fiscal Year: 2010

Scientific Experts

Detail Information

Publications85

  1. ncbi Regions of variant histone His2AvD required for Drosophila development
    M J Clarkson
    The John Curtin School of Medical Research, The Australian National University, Canberra, Australian Capital Territory
    Nature 399:694-7. 1999
    ..domain could be established to carry out a specialized function is by the localized incorporation of specific histone variants into nucleosomes...
  2. ncbi Drosophila rasiRNA pathway mutations disrupt embryonic axis specification through activation of an ATR/Chk2 DNA damage response
    Carla Klattenhoff
    Program in Molecular Medicine and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Dev Cell 12:45-55. 2007
    ..Furthermore, rasiRNA pathway mutations lead to germline-specific accumulation of gamma-H2Av foci characteristic of DNA damage...
  3. ncbi A histone variant, H2AvD, is essential in Drosophila melanogaster
    A van Daal
    Department of Biology, Washington University, St Louis, Missouri 63130
    Mol Biol Cell 3:593-602. 1992
    H2AvD, a Drosophila melanogaster histone variant of the H2A.Z class, is encoded by a single copy gene in the 97CD region of the polytene chromosomes...
  4. ncbi DNA double-strand break-induced phosphorylation of Drosophila histone variant H2Av helps prevent radiation-induced apoptosis
    James P Madigan
    Wadsworth Center, New York State Department of Health, State University of New York, Albany, NY 12201 2002, USA
    Nucleic Acids Res 30:3698-705. 2002
    ..We demonstrate the DSB-induced phosphorylation of histone variant H2Av in Drosophila melanogaster. H2Av is a member of the H2AZ family of histone variants...
  5. ncbi Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions
    Thomas Kusch
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Science 306:2084-7. 2004
    Phosphorylation of the human histone variant H2A.X and H2Av, its homolog in Drosophila melanogaster, occurs rapidly at sites of DNA double-strand breaks...
  6. ncbi The degradation of two mitotic cyclins contributes to the timing of cytokinesis
    Arnaud Echard
    Department of Biochemistry and Biophysics, University of California, San Francisco, Genentech Hall, 600 16th Street, San Francisco, CA 94143, USA
    Curr Biol 13:373-83. 2003
    ..We examined the interface between mitotic cyclin destruction and the timing of cytokinesis...
  7. ncbi Relationship of DNA double-strand breaks to synapsis in Drosophila
    Janet K Jang
    Waksman Institute and Department of Genetics, Rutgers, The State University of New Jersey, 190 Frelinghuysen Rd, Piscataway, New Jersey 08854 8020, USA
    J Cell Sci 116:3069-77. 2003
    ..requirements for double-strand break formation during Drosophila meiosis, we used an antibody that recognizes a histone modification at double-strand break sites, phosphorylation of HIS2AV (gamma-HIS2AV)...
  8. ncbi Temporal analysis of meiotic DNA double-strand break formation and repair in Drosophila females
    S Mehrotra
    Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America
    PLoS Genet 2:e200. 2006
    Using an antibody against the phosphorylated form of His2Av (gamma-His2Av), we have described the time course for the series of events leading from the formation of a double-strand break (DSB) to a crossover in Drosophila female meiotic ..
  9. ncbi Onset of the DNA replication checkpoint in the early Drosophila embryo
    Justin Crest
    Department of Biology, University of Washington, Seattle, Washington 98195 1800, USA
    Genetics 175:567-84. 2007
    ..Our results suggest an antagonistic interaction between DNA replication checkpoint activation and Cdk1-CycB activity during the transition from preblastoderm to blastoderm cycles...
  10. ncbi Both cyclin B levels and DNA-replication checkpoint control the early embryonic mitoses in Drosophila
    Jun Yuan Ji
    Department of Biology, University of Washington, Seattle, WA 98195 1800, USA
    Development 131:401-11. 2004
    ....
  11. ncbi Drosophila mus301/spindle-C encodes a helicase with an essential role in double-strand DNA break repair and meiotic progression
    Ruth McCaffrey
    MRC Laboratory of Molecular Biology, The Gurdon Institute and Department of Genetics, University of Cambridge, Cambridge CB2 1QR, United Kingdom
    Genetics 174:1273-85. 2006
    ..However, neither mei-W68 nor mei-41 rescue the defects in oocyte specification of mus301 mutants, suggesting that this helicase has another function in oocyte selection that is independent from its role in meiotic recombination...
  12. ncbi Greatwall kinase: a nuclear protein required for proper chromosome condensation and mitotic progression in Drosophila
    Jiangtao Yu
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853 2703, USA
    J Cell Biol 164:487-92. 2004
    ..Chromosomes are highly undercondensed, particularly in the euchromatin, but nevertheless contain phosphorylated histone H3, condensin, and topoisomerase II...
  13. ncbi Regulation of mitosis in response to damaged or incompletely replicated DNA require different levels of Grapes (Drosophila Chk1)
    Amanda Purdy
    MCD Biology, University of Colorado, Boulder, CO 80309 0347, USA
    J Cell Sci 118:3305-15. 2005
    ..Finally, our results demonstrate that Drosophila provides a unique opportunity to study the regulation of the entry into, and progress through, mitosis by DNA structure checkpoints in metazoa...
  14. ncbi Drosophila Wee1 kinase regulates Cdk1 and mitotic entry during embryogenesis
    Jason Stumpff
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Curr Biol 14:2143-8. 2004
    ..These findings modify previous models about cell cycle regulation in syncytial embryos and demonstrate that Wee1 kinases can regulate mitotic entry in vivo during metazoan development even in cycles that lack a G2 phase...
  15. ncbi The role of histone H2Av variant replacement and histone H4 acetylation in the establishment of Drosophila heterochromatin
    Jyothishmathi Swaminathan
    Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Genes Dev 19:65-76. 2005
    ..Here we show that the histone H2A variant of Drosophila melanogaster, H2Av, localizes to the centromeric heterochromatin, and it is recruited to an ..
  16. ncbi Replacement by Drosophila melanogaster protamines and Mst77F of histones during chromatin condensation in late spermatids and role of sesame in the removal of these proteins from the male pronucleus
    Sunil Jayaramaiah Raja
    Philipps Universitat Marburg, Fachbereich Biologie, Entwicklungsbiologie, 35043 Marburg, Germany
    Mol Cell Biol 25:6165-77. 2005
    ..Mst77F encodes a linker histone-like protein showing significant similarity to mammalian HILS1 protein...
  17. ncbi Incorporation of Drosophila CID/CENP-A and CENP-C into centromeres during early embryonic anaphase
    Melina Schuh
    Bayreuth Center for Molecular Biosciences BZMB, Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany
    Curr Biol 17:237-43. 2007
    ..A candidate for an epigenetic mark is the centromere-specific histone H3 variant CENP-A that replaces H3 in alternating blocks of chromatin exclusively in active centromeres...
  18. ncbi A His2AvDGFP fusion gene complements a lethal His2AvD mutant allele and provides an in vivo marker for Drosophila chromosome behavior
    M Clarkson
    Department of Biochemistry, University of Adelaide, SA, Australia
    DNA Cell Biol 18:457-62. 1999
    ..melanogaster variant H2A.F/Z class histone, His2AvD, fused to the green fluorescent protein (GFP) of the jellyfish Aequorea victoria...
  19. ncbi Nucleosome organization in the Drosophila genome
    Travis N Mavrich
    Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Nature 453:358-62. 2008
    ..Here we produce a high-resolution reference map of H2A.Z and bulk nucleosome locations across the genome of the fly Drosophila melanogaster and compare it to that from ..
  20. ncbi RNAi of mitotic cyclins in Drosophila uncouples the nuclear and centrosome cycle
    Mark L McCleland
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California 94143 2200, USA
    Curr Biol 18:245-54. 2008
    ..However, we still know very little of how individual cell-cycle events are coupled to this oscillator and how the timing of each event is controlled...
  21. ncbi The schedule of destruction of three mitotic cyclins can dictate the timing of events during exit from mitosis
    D H Parry
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
    Curr Biol 11:671-83. 2001
    ..We tested a recent proposal that the destruction of the different cyclins guides progress through mitosis...
  22. ncbi Histone H2A.Z is widely but nonrandomly distributed in chromosomes of Drosophila melanogaster
    T J Leach
    Wadsworth Center, New York State Department of Health and Department of Biomedical Sciences, State University of New York, Albany, New York 12201 2002, USA
    J Biol Chem 275:23267-72. 2000
    ..H2A.F/Z is a highly conserved family of H2A variants. H2Av, the H2A...
  23. ncbi Acf1 confers unique activities to ACF/CHRAC and promotes the formation rather than disruption of chromatin in vivo
    Dmitry V Fyodorov
    Section of Molecular Biology, University of California at San Diego, La Jolla, CA 92093, USA
    Genes Dev 18:170-83. 2004
    ..These findings collectively indicate that ACF/CHRAC functions in the assembly of periodic nucleosome arrays that contribute to the repression of genetic activity in the eukaryotic nucleus...
  24. ncbi Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation
    Anne Royou
    Department of Molecular Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    J Cell Biol 183:63-75. 2008
    ..Grp(Chk1) prevents nuclear CDK1 activation by delaying CycB nuclear accumulation through Wee1-dependent and independent mechanisms...
  25. ncbi The cohesion protein ORD is required for homologue bias during meiotic recombination
    Hayley A Webber
    Dept of Biological Sciences, Dartmouth College, 6044 Gilman, Hanover, NH 03755, USA
    J Cell Biol 164:819-29. 2004
    ..We conclude that ORD activity suppresses sister chromatid exchange and stimulates inter-homologue crossovers, thereby promoting homologue bias during meiotic recombination in Drosophila...
  26. ncbi Gawky is a component of cytoplasmic mRNA processing bodies required for early Drosophila development
    Mary D Schneider
    Department of Cell Biology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G H7, Canada
    J Cell Biol 174:349-58. 2006
    ..This suggests that gw represents a previously unknown member of a small group of genes that need to be expressed zygotically during early embryo development...
  27. ncbi Somatic stem cell niche tropism in Wolbachia
    Horacio M Frydman
    Howard Hughes Medical Institute, Princeton University, Princeton, New Jersey 08540, USA
    Nature 441:509-12. 2006
    ..This is, to our knowledge, the first report of an intracellular parasite displaying tropism for a stem cell niche...
  28. ncbi Expression of COPI components during development of Drosophila melanogaster
    Nicole C Grieder
    Biozentrum der Universitat Basel, Abteilung Zellbiologie, Klingelbergstrasse 50 70, CH 4056 Basel, Switzerland
    Gene Expr Patterns 6:11-21. 2005
    ....
  29. ncbi A genome-wide RNA interference screen identifies putative chromatin regulators essential for E2F repression
    Jianrong Lu
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:9381-6. 2007
    ..These findings suggest that potential chromatin regulation mediated by Domino and PcG-like factors plays an important role in controlling E2F activity and cell growth...
  30. ncbi The Drosophila hus1 gene is required for homologous recombination repair during meiosis
    Gabriella Peretz
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University, POB 653, Beer Sheva 84105, Israel
    Mech Dev 126:677-86. 2009
    ..Together, our results imply that hus1 is required for repair of DSBs during meiotic recombination...
  31. ncbi The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clusters
    Carla Klattenhoff
    University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 138:1137-49. 2009
    ..Rhino thus appears to promote transcription of dual-strand clusters, leading to production of piRNAs that drive the ping-pong amplification cycle...
  32. ncbi Transcriptional and developmental functions of the H3.3 histone variant in Drosophila
    Akiko Sakai
    Department of BCMP, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Curr Biol 19:1816-20. 2009
    ..In most animals, replacement uses the conserved H3.3 histone variant, but the functions of this variant have not been defined. Using mutations for the two H3...
  33. ncbi Cellular mechanisms of dendrite pruning in Drosophila: insights from in vivo time-lapse of remodeling dendritic arborizing sensory neurons
    Darren W Williams
    Department of Biology, University of Washington, Seattle, WA 98195, USA
    Development 132:3631-42. 2005
    ..Finally, we find that phagocytic blood cells not only engulf neuronal debris but also attack and sever intact branches that show signs of destabilization...
  34. ncbi Mago Nashi and Tsunagi/Y14, respectively, regulate Drosophila germline stem cell differentiation and oocyte specification
    David H Parma
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Dev Biol 308:507-19. 2007
    ..On the other hand, Tsunagi/Y14 is essential for restricting oocyte fate to a single cell and may function with mago nashi in this process...
  35. ncbi Identification of EMS-induced mutations in Drosophila melanogaster by whole-genome sequencing
    Justin P Blumenstiel
    Department of Physiology, Kansas University Medical Center, Kansas City, Kansas 66160, USA
    Genetics 182:25-32. 2009
    ..This new method of whole-genome sequencing represents great promise for mutant mapping in flies, potentially replacing conventional methods...
  36. ncbi The cyclin-dependent kinase inhibitor Dacapo promotes replication licensing during Drosophila endocycles
    Amy Hong
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 26:2071-82. 2007
    ..Finally, genetic interaction studies suggest that dap functions to promote replication licensing in a subset of Drosophila mitotic cycles...
  37. ncbi Painting of fourth and chromosome-wide regulation of the 4th chromosome in Drosophila melanogaster
    Anna Mia Johansson
    UCMP, Umea University, Umea, Sweden
    EMBO J 26:2307-16. 2007
    ..We propose a balancing mechanism involving POF and HP1 that provides a feedback system for fine-tuning expression status of genes on the 4th chromosome...
  38. ncbi Aberrant monomethylation of histone H4 lysine 20 activates the DNA damage checkpoint in Drosophila melanogaster
    Ayako Sakaguchi
    Waksman Institute, Department of Molecular Biology and Biochemistry, Cancer Institute of New Jersey, Rutgers University, Piscataway, NJ 08854, USA
    J Cell Biol 176:155-62. 2007
    PR-Set7 is a histone methyltransferase that specifically monomethylates histone H4 lysine 20 (K20) and is essential for cell proliferation. Our results show that in PR-Set7 mutants, the DNA damage checkpoint is activated...
  39. ncbi A Distinct type of heterochromatin within Drosophila melanogaster chromosome 4
    Karmella A Haynes
    Department of Biology, Washington University, St Louis, Missouri 63130, USA
    Genetics 175:1539-42. 2007
    ..distal arm chromosome 4 heterochromatin, distinguishing these two heterochromatin types...
  40. ncbi Loss of the histone variant H2A.Z restores capping to checkpoint-defective telomeres in Drosophila
    Yikang S Rong
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genetics 180:1869-75. 2008
    The conserved histone variant H2A.Z fulfills many functions by being an integral part of the nucleosomes placed at specific regions of the genome...
  41. ncbi Drosophila Aurora-A kinase inhibits neuroblast self-renewal by regulating aPKC/Numb cortical polarity and spindle orientation
    Cheng Yu Lee
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon 97403, USA
    Genes Dev 20:3464-74. 2006
    ..We conclude that Aurora-A and Numb are novel inhibitors of neuroblast self-renewal and that spindle orientation regulates neuroblast self-renewal...
  42. ncbi Drosophila PCH2 is required for a pachytene checkpoint that monitors double-strand-break-independent events leading to meiotic crossover formation
    Eric F Joyce
    Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854 8020, USA
    Genetics 181:39-51. 2009
    ..Interestingly, PCH2-dependent delays in prophase may allow additional crossovers to form...
  43. ncbi Influence of cyclin type and dose on mitotic entry and progression in the early Drosophila embryo
    Mark L McCleland
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
    J Cell Biol 184:639-46. 2009
    ....
  44. ncbi Drosophila ORC localizes to open chromatin and marks sites of cohesin complex loading
    Heather K MacAlpine
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Genome Res 20:201-11. 2010
    ..ORC-associated sequences are enriched for the histone variant, H3.3, often at transcription start sites, and depleted for bulk nucleosomes...
  45. ncbi Mitosis in primary cultures of Drosophila melanogaster larval neuroblasts
    Matthew S Savoian
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, P O Box 509, Albany, New York 12201 0509, USA
    J Cell Sci 115:3061-72. 2002
    ..5 minutes. Analysis of neuroblasts containing various GFP-tagged proteins (e.g. histone, fizzy, fizzy-related and alpha-tubulin) reveals that attaching kinetochores exhibit sudden, rapid pole-directed ..
  46. ncbi Fluorescence-activated cell sorting (FACS) of Drosophila hemocytes reveals important functional similarities to mammalian leukocytes
    Rabindra Tirouvanziam
    Herzenberg Laboratory, Department of Genetics, Stanford University School of Medicine, Beckman Center B007, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 101:2912-7. 2004
    ....
  47. ncbi Contribution of noncentrosomal microtubules to spindle assembly in Drosophila spermatocytes
    Elena Rebollo
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS Biol 2:E8. 2004
    ..These observations are consistent with a model in which centrosomal and noncentrosomal microtubules contribute to the assembly and are required for the robustness of the cell division spindle in cells that contain centrosomes...
  48. ncbi Conservation of intron position indicates separation of major and variant H2As is an early event in the evolution of eukaryotes
    A van Daal
    Department of Biology, Washington University, St Louis, Missouri 63130
    J Mol Evol 30:449-55. 1990
    ..of the variant and major cell cycle-regulated histone H2A proteins from several species indicates that the H2A variant proteins are evolutionarily separate and distinct from the major cell cycle-regulated histone H2A proteins...
  49. ncbi The dual function of ovo/shavenbaby in germline and epidermis differentiation is conserved between Drosophila melanogaster and the olive fruit fly Bactrocera oleae
    Abderrahman Khila
    Laboratoire de Biotechnologie Végétale et Agro Alimentaire, Faculté des Sciences et Techniques de Fès Saïss, Université Sidi Mohammed Ben Abdellah, route de Imouzer, BP2202 Fès, Morocco
    Insect Biochem Mol Biol 33:691-9. 2003
    ..oleae svb embryonic expression precisely prefigures the pattern of larval trichomes, supporting the conclusion that regulatory changes in svb transcription underlie evolutionary diversification of trichome patterns seen among Dipterae...
  50. ncbi The Drosophila EAST protein associates with a nuclear remnant during mitosis and constrains chromosome mobility
    Martin Wasser
    Institute of Molecular and Cell Biology, National University of Singapore, 30 Medical Drive, Singapore 117609
    J Cell Sci 116:1733-43. 2003
    ..We propose that EAST constitutes a component of a nucleoskeleton that helps to constrain the mobility of chromosomes in interphase, mitosis and meiosis...
  51. ncbi The centrosome is a dynamic structure that ejects PCM flares
    Timothy L Megraw
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    J Cell Sci 115:4707-18. 2002
    ..We propose that flare particles play a role in organizing the actin cytoskeleton during syncytial cleavage...
  52. ncbi The cyclin-dependent kinase inhibitor Roughex is involved in mitotic exit in Drosophila
    E Foley
    Institut fur Genetik, Universitat zu Koln, Weyertal 121, D 50931, Koln, Germany
    Curr Biol 11:151-60. 2001
    ..In contrast, the only mechanism known to be essential for CDK inactivation during mitosis in higher eukaryotes is cyclin destruction...
  53. ncbi A complex degradation signal in Cyclin A required for G1 arrest, and a C-terminal region for mitosis
    H W Jacobs
    Department of Genetics, University of Bayreuth, D 95440 Bayreuth, Germany
    EMBO J 20:2376-86. 2001
    ..However, we demonstrate that this failure of mitosis is not caused simply by DNA replication or damage checkpoints. Entry into mitosis requires a function of Cyclin A that does not depend on the presence of the N-terminal region...
  54. ncbi Toucan protein is essential for the assembly of syncytial mitotic spindles in Drosophila melanogaster
    A Debec
    Observatoire Oceanologique, Laboratoire de Biologie du Developpement, Université Pierre et Marie Curie CNRS, Villefranche sur Mer, France
    Genesis 31:167-75. 2001
    ..These results strongly suggest that Toucan represents a factor essential for the assembly and the function of the syncytial mitotic spindles...
  55. ncbi Activation of the cAMP/PKA signaling pathway is required for post-ecdysial cell death in wing epidermal cells of Drosophila melanogaster
    Ken ichi Kimura
    Laboratory of Biology, Iwamizawa Campus, Hokkaido University of Education, Iwamizawa, Hokkaido 068 8642, Japan
    Development 131:1597-606. 2004
    ..Thus, activation of the cAMP/PKA signaling pathway is required for transduction of the hormonal signal that induces wing epidermal cell death after eclosion...
  56. ncbi RSF governs silent chromatin formation via histone H2Av replacement
    Kazuma Hanai
    Department of Developmental Genetics, National Institute of Genetics, Shizuoka ken, Japan
    PLoS Genet 4:e1000011. 2008
    ..In dRsf-1 mutant, the levels of histone H3K9 dimethylation and histone H2A variant H2Av were significantly reduced in an euchromatic region juxtaposed with heterochromatin...
  57. ncbi Epithelial re-organization and dynamics of progression through mitosis in Drosophila separase complex mutants
    Rahul Pandey
    BZMB, Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany
    J Cell Sci 118:733-42. 2005
    ....
  58. ncbi All paired up with no place to go: pairing, synapsis, and DSB formation in a balancer heterozygote
    Wei J Gong
    Stowers Institute for Medical Research, Kansas City, Missouri, United States of America
    PLoS Genet 1:e67. 2005
    ....
  59. ncbi The mitotic arrest in response to hypoxia and of polar bodies during early embryogenesis requires Drosophila Mps1
    Matthias G Fischer
    BZMB, Department of Genetics, University of Bayreuth, 94440 Bayreuth, Germany
    Curr Biol 14:2019-24. 2004
    ..Finally, we show that Mps1 and the mitotic spindle checkpoint are responsible for the developmental cell cycle arrest of the three haploid products of female meiosis that are not used as the female pronucleus...
  60. ncbi The EAST protein of drosophila controls an expandable nuclear endoskeleton
    M Wasser
    Institute of Molecular and Cell Biology, National University of Singapore
    Nat Cell Biol 2:268-75. 2000
    ..We propose that EAST mediates the assembly of an expandable nuclear endoskeleton which, through alterations of its volume, can modulate the spatial arrangement of chromosomes...
  61. ncbi The generation of cloned Drosophila melanogaster
    Andrew J Haigh
    Department of Biology, Dalhousie University, Halifax, Nova Scotia B4H 4J1, Canada
    Genetics 169:1165-7. 2005
    ....
  62. ncbi Genetic characterization of ms (3) K81, a paternal effect gene of Drosophila melanogaster
    G K Yasuda
    Department of Zoology, University of Washington, Seattle 98195 1800, USA
    Genetics 140:219-29. 1995
    ..Based on the embryonic lethal phenotypes, we suggest that the K81+ gene encodes a sperm-specific product that is essential for the male pronucleus to participate in the first few embryonic nuclear divisions...
  63. ncbi The importin-beta P446L dominant-negative mutant protein loses RanGTP binding ability and blocks the formation of intact nuclear envelope
    Gyula Timinszky
    The University of Szeged, Faculty of Medicine, Department of Biology, Somogyi B u 4, H 6720 Szeged, Hungary
    J Cell Sci 115:1675-87. 2002
    ..However, it interferes with nuclear envelope formation during mitosis-to-interphase transition, revealing a novel function of importin-beta...
  64. ncbi Genetic analysis of chromosomal region 97D2-9 of Drosophila melanogaster
    W R Knibb
    Department of Biochemistry, University of Adelaide, Australia
    Mol Gen Genet 239:109-14. 1993
    ..In addition to rough, one other locus within this region, 1(3)97De, was shown to be required for formation of the normal pattern of photoreceptor cells in the compound eye...
  65. ncbi DNA damage leads to a Cyclin A-dependent delay in metaphase-anaphase transition in the Drosophila gastrula
    T T Su
    MCD Biology, University of Colorado, Boulder, CO 80309, USA
    Curr Biol 11:8-17. 2001
    ..The resulting polyploid nuclei are eliminated...
  66. ncbi Cytoplasmic dynein is required for poleward chromosome movement during mitosis in Drosophila embryos
    D J Sharp
    Section of Molecular and Cellular Biology, University of California Davis, 1 Shields Avenue, Davis, California 95616, USA
    Nat Cell Biol 2:922-30. 2000
    ..Thus, dynein is essential for poleward chromosome motility throughout mitosis in Drosophila embryos...
  67. ncbi Hypoxia and nitric oxide induce a rapid, reversible cell cycle arrest of the Drosophila syncytial divisions
    P J DiGregorio
    Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143, USA
    J Biol Chem 276:1930-7. 2001
    ..We examined this rapid arrest in real time using a fusion of green fluorescent protein and histone 2A. In addition to an interphase arrest, mitosis was specifically blocked in metaphase, much like a checkpoint ..
  68. ncbi Activation of facultatively silenced Drosophila loci associates with increased acetylation of histone H2AvD
    Masahiko Tanabe
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo, Japan
    Genes Cells 13:1279-88. 2008
    H2A.Z is an evolutionarily highly conserved non-allelic variant of histone H2A. H2A.Z and its homologues have been shown to involve in both chromatin silencing and activation. Although much of our knowledge of H2A...
  69. ncbi Pictures in cell biology. A functional marker for Drosophila chromosomes in vivo
    R Saint
    Centre for the Molecular Genetics of Development and Dept of Molecular Biosciences, University of Adelaide, SA 5005, Adelaide, Australia
    Trends Cell Biol 10:553. 2000
  70. ncbi grp (chk1) replication-checkpoint mutations and DNA damage trigger a Chk2-dependent block at the Drosophila midblastula transition
    Saeko Takada
    Program in Molecular Medicine, Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Development 134:1737-44. 2007
    ..We conclude that the DNA-replication checkpoint maintains genome integrity during the cleavage divisions, and that checkpoint mutations lead to DNA damage that induces a novel Chk2-dependent block at the MBT...
  71. ncbi Synthesis of the sulfate donor PAPS in either the Drosophila germline or somatic follicle cells can support embryonic dorsal-ventral axis formation
    Xianjun Zhu
    Section of Molecular Cell and Developmental Biology, University of Texas at Austin, Austin, TX 78712, USA
    Development 134:1465-9. 2007
    ..This finding indicates the existence of a conduit for the movement of PAPS between the germline and the follicle cells, which highlights a previously unappreciated mechanism of soma/germline cooperation affecting pattern formation...
  72. ncbi Genetic interactions of separase regulatory subunits reveal the diverged Drosophila Cenp-C homolog
    Sebastian Heeger
    Department of Genetics, BZMB, University of Bayreuth, 95440 Bayreuth, Germany
    Genes Dev 19:2041-53. 2005
    ....
  73. ncbi Maternal expression of the checkpoint protein BubR1 is required for synchrony of syncytial nuclear divisions and polar body arrest in Drosophila melanogaster
    Daniel Pérez-Mongiovi
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    Development 132:4509-20. 2005
    ..Our results suggest that BubR1 is required for normal synchrony and progression of syncytial nuclei through mitosis and to maintain the mitotic arrest of the polar body chromosomes after completion of meiosis...
  74. ncbi A histone code in meiosis: the histone kinase, NHK-1, is required for proper chromosomal architecture in Drosophila oocytes
    Irena Ivanovska
    Whitehead Institute and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Genes Dev 19:2571-82. 2005
    ..We describe a mutation in a recently identified Histone H2A kinase, nhk-1, in Drosophila that leads to female sterility due to defects in the formation of the meiotic ..
  75. ncbi One- and two-photon photoactivation of a paGFP-fusion protein in live Drosophila embryos
    Janine N Post
    Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    FEBS Lett 579:325-30. 2005
    We constructed a photoactivatable Drosophila histone 2 A variant green fluorescent fusion protein (H2AvD-paGFP) for tracking chromatin loci in living Drosophila embryos...
  76. ncbi The KASH domain protein MSP-300 plays an essential role in nuclear anchoring during Drosophila oogenesis
    Juehua Yu
    Institute of Developmental Biology and Molecular Medicine, Morgan Tan International Center for Life Sciences, School of Life Science, Fudan University, Shanghai, China
    Dev Biol 289:336-45. 2006
    ..These results indicate that maternal MSP-300 plays an important role in actin-dependent nuclear anchorage during cytoplasmic transport...
  77. ncbi Postsynaptic mad signaling at the Drosophila neuromuscular junction
    Veronica Dudu
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Curr Biol 16:625-35. 2006
    ..There is evidence that a retrograde, presynaptic transforming growth factor-beta (TGF-beta) signaling event regulates synapse development and function in Drosophila...
  78. ncbi Developmental biology: holding pattern for histones
    Dawn L Brasaemle
    Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
    Curr Biol 16:R918-20. 2006
    ..New research on lipid droplets in Drosophila embryos has led to the surprising conclusion that these poorly understood organelles have a novel role as a regulated storage depot of maternally supplied proteins, particularly histones...
  79. ncbi The chromokinesin, KLP3A, dives mitotic spindle pole separation during prometaphase and anaphase and facilitates chromatid motility
    Mijung Kwon
    University of California Davis, Davis, California 95616, USA
    Mol Biol Cell 15:219-33. 2004
    ..We propose that KLP3A acts on MTs associated with chromosome arms and the central spindle to organize ipMT bundles, to drive spindle pole separation and to facilitate chromatid motility...
  80. ncbi Control of cleavage cycles in Drosophila embryos by frühstart
    Jörg Grosshans
    ZMBH, Universitat Heidelberg, Im Neuenheimer Feld 282, 69120, Heidelberg, Germany
    Dev Cell 5:285-94. 2003
    ..Furthermore, the expression of frs is delayed in haploid embryos and requires activity of the maternal checkpoint gene grapes. We propose that zygotic frs expression is involved in linking the N/C and the pause of cleavage cycle...
  81. ncbi Drosophila APC2 and APC1 have overlapping roles in the larval brain despite their distinct intracellular localizations
    Kathryn Akong
    Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Dev Biol 250:71-90. 2002
    ..Our experiments suggest that this does not result from misregulation of Wg signaling, and thus may involve the cytoskeletal or adhesive roles of APC proteins...
  82. ncbi Completion of mitosis requires neither fzr/rap nor fzr2, a male germline-specific Drosophila Cdh1 homolog
    Henning Jacobs
    Department of Genetics, University of Bayreuth, 95440, Bayreuth, Germany
    Curr Biol 12:1435-41. 2002
    ..However, fzr is an essential gene corresponding to the rap locus, and FZR, which accumulates predominantly in the cytoplasm, is clearly required during G1...
  83. ncbi Ran localizes around the microtubule spindle in vivo during mitosis in Drosophila embryos
    Nadia Trieselmann
    Department of Medical Genetics and Microbiology, University of Toronto, 1 King s College Circle, Ontario M5S 1A8, Canada
    Curr Biol 12:1124-9. 2002
    ..In contrast, nuclear proteins redistribute throughout the embryo upon nuclear envelope breakdown (NEB). Thus, in vivo RanGTP has the correct spatial localization within the cell to modulate spindle assembly...
  84. ncbi Genetic interactions between Cdk1-CyclinB and the Separase complex in Drosophila
    Jun Yuan Ji
    Department of Biology, University of Washington, Seattle, WA 98195 1800, USA
    Development 132:1875-84. 2005
    ....
  85. ncbi Characterisation of T48, a target of homeotic gene regulation in Drosophila embryogenesis
    D I Strutt
    Department of Anatomy, University of Cambridge, UK
    Mech Dev 46:27-39. 1994
    ..Therefore, we suggest that this is an example of a direct target of regulation by homeotic genes in two germlayers...

Research Grants56

  1. Cell Growth and Radiation Survival in Drosophila
    Tin Tin Su; Fiscal Year: 2007
    ..Understanding radiation survival in a multicellular context could help identify potential therapies for when we do not want cells to survive irradiation, such as in the treatment of cancer. ..
  2. Regulation of Radiation Induced Cell Death in Drosophila
    Tin Tin Su; Fiscal Year: 2010
    ..Because of excellent conservation of gene function between Drosophila and human, what we learn may allow us to maximize the efficacy of radiation therapy of human cancers. ..
  3. Perilipins and cellular triacyglycerol metabolism
    Dawn Brasaemle; Fiscal Year: 2007
    ..These studies will contribute to a long-term goal of defining the factors on lipid droplets that control adipocyte TAG metabolism. ..
  4. An in vivo Screen for Radiation Sensitizers
    Tin Tin Su; Fiscal Year: 2007
    ..Successful completion of this project would establish a novel, rapid and effective primary screen in vivo for radiosensitizers with potential therapeutic value. ..
  5. Maintenance of blocks-based tools for functional genomic
    Steven Henikoff; Fiscal Year: 2006
    ..These various components will be packaged to facilitate updates and local implementations. ..
  6. Cell Cycle Regulation by Checkpoints in Drosophila
    Tin Tin Su; Fiscal Year: 2006
    ..Aim 4) We will screen through existing mutagensensitive Drosophila mutants for their ability to regulate mitosis, S phase and cell death after irradiation, in order to uncover novel genes needed for proper response to DNA damage. ..
  7. Mini spindles and microtubule function in Drosophila
    NANCY POKRYWKA; Fiscal Year: 2006
    ....
  8. Genome-wide profiling of histone variants in Drosophila and Caenorhabditis
    Steven Henikoff; Fiscal Year: 2007
    DESCRIPTION (provided by the applicant): We propose to map major core histone variants throughout the annotated Drosophila melanogaster and Caenorhabditis elegans genomes at high resolution...
  9. Epigenomic profiling of histone turnover kinetics in mammalian cells
    Steven Henikoff; Fiscal Year: 2010
    Epigenomic profiling of histone turnover kinetics in mammalian cells We have developed a revolutionary technology, CATCH-IT (for Covalent Attachment of Tags to Capture Histones and Identify Turnover) to directly measure nucleosome ..
  10. Perilipins and cellular triacyglycerol metabolism
    Dawn L Brasaemle; Fiscal Year: 2010
    ..These studies will contribute to a long-term goal of defining the factors on lipid droplets that control adipocyte TAG metabolism. ..
  11. Perilipin and cellular triacylglycerol metabolism
    Dawn L Brasaemle; Fiscal Year: 2010
    ....
  12. Regulation of Radiation Induced Cell Death in Drosophila
    Tin Tin Su; Fiscal Year: 2010
    ..Because of excellent conservation of gene function between Drosophila and human, what we learn may allow us to maximize the efficacy of radiation therapy of human cancers. ..
  13. Perilipins and cellular triacyglycerol metabolism
    Dawn Brasaemle; Fiscal Year: 2009
    ..These studies will contribute to a long-term goal of defining the factors on lipid droplets that control adipocyte TAG metabolism. ..
  14. Lipid Droplets: Metabolic Consequences of Stored Neutral Lipids
    Dawn Brasaemle; Fiscal Year: 2007
    ....
  15. GENE REGULATION OF THE PURINE SYNTHETIC PATHWAY
    Steven Henikoff; Fiscal Year: 1993
    ..It is likely that some of these other genes that we identify by this approach will correspond to loci that were previously identified by mutation, thus furthering our understanding of the relationship between phenotype and gene function...
  16. PERILIPINS AND CELLULAR TRIACYLGLYCEROL METABOLISM
    Dawn Brasaemle; Fiscal Year: 2003
    ..These aims will be tested by the expression of intact and mutant perilipin A in cultured cells that lack perilipins followed by assays for lipid storage and release, and the presence of the mutant perilipins on lipid droplets. ..
  17. Mechanisms of Swallow protein function in Drosophila
    NANCY POKRYWKA; Fiscal Year: 2002
    ....
  18. ALIGNMENT BASED METHODS FOR MOLECULAR SEQUENCE ANALYSIS
    Steven Henikoff; Fiscal Year: 2001
    ..This effort could yield new eukaryotic family members of great importance for understanding epigenetic phenomena, some of which, such as parental imprinting, are relevant to human disease. ..