Gene Symbol: Hen1
Description: Hen1 methyltransferase
Alias: CG12367, DmHen1, Dmel\CG12367, FBgn 33686, PIMET, Pimet, Pimet/DmHen1, hen-1, hen1, Hen1 methyltransferase, CG12367-PA, Hen1-PA, piRNA methyltransferase
Species: fruit fly

Top Publications

  1. Okamura K, Robine N, Liu Y, Liu Q, Lai E. R2D2 organizes small regulatory RNA pathways in Drosophila. Mol Cell Biol. 2011;31:884-96 pubmed publisher
    ..Together, these data reveal new roles of R2D2 in organizing small RNA networks in Drosophila. ..
  2. Saito K, Sakaguchi Y, Suzuki T, Suzuki T, Siomi H, Siomi M. Pimet, the Drosophila homolog of HEN1, mediates 2'-O-methylation of Piwi- interacting RNAs at their 3' ends. Genes Dev. 2007;21:1603-8 pubmed
    ..Here we show that piRNAs in Drosophila are 2'-O-methylated at their 3' ends. Loss of Pimet (piRNA methyltransferase), the Drosophila homolog of Arabidopsis HEN1 methyltransferase for microRNAs (miRNAs), results in loss of ..
  3. Ameres S, Horwich M, Hung J, Xu J, Ghildiyal M, Weng Z, et al. Target RNA-directed trimming and tailing of small silencing RNAs. Science. 2010;328:1534-9 pubmed publisher
    ..This modification blocks target-directed small RNA remodeling: In flies lacking Hen1, the enzyme that adds the 2'-O-methyl group, Argonaute2-associated siRNAs are tailed and trimmed...
  4. Abe M, Naqvi A, Hendriks G, Feltzin V, Zhu Y, Grigoriev A, et al. Impact of age-associated increase in 2'-O-methylation of miRNAs on aging and neurodegeneration in Drosophila. Genes Dev. 2014;28:44-57 pubmed publisher
    ..Mutations in Hen1 and Ago2, which lack 2'-O-methylation of miRNAs, result in accelerated neurodegeneration and shorter life span, ..
  5. Ameres S, Hung J, Xu J, Weng Z, Zamore P. Target RNA-directed tailing and trimming purifies the sorting of endo-siRNAs between the two Drosophila Argonaute proteins. RNA. 2011;17:54-63 pubmed publisher
  6. Horwich M, Li C, Matranga C, Vagin V, Farley G, Wang P, et al. The Drosophila RNA methyltransferase, DmHen1, modifies germline piRNAs and single-stranded siRNAs in RISC. Curr Biol. 2007;17:1265-72 pubmed
    ..Plant small-RNA methylation is catalyzed by the protein HEN1 [10, 14, 15]. We find that DmHen1, the Drosophila homolog of HEN1, methylates the termini of siRNAs and piRNAs...
  7. Okamura K, Chung W, Ruby J, Guo H, Bartel D, Lai E. The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs. Nature. 2008;453:803-6 pubmed publisher
    ..hpRNA pathway is a hybrid mechanism that combines canonical RNA interference factors (Dicer-2, Hen1 (known as CG12367) and Argonaute 2) with a canonical microRNA factor (Loquacious) to generate approximately 21-nucleotide siRNAs...
  8. Yu Z, Teng X, Bonini N. Triplet repeat-derived siRNAs enhance RNA-mediated toxicity in a Drosophila model for myotonic dystrophy. PLoS Genet. 2011;7:e1001340 pubmed publisher
    ..These findings indicate that the generation of triplet repeat-derived siRNAs may dramatically enhance toxicity in human repeat expansion diseases in which anti-sense transcription occurs...
  9. Bronkhorst A, Van Rij R. The long and short of antiviral defense: small RNA-based immunity in insects. Curr Opin Virol. 2014;7:19-28 pubmed publisher
    ..Here, we review recent insights into the mechanism of antiviral RNAi, viral small RNA profiles, and viral counter-defense mechanisms in insects. ..

More Information


  1. McCracken A, Locke J. Mutations in CG8878, a novel putative protein kinase, enhance P element dependent silencing (PDS) and position effect variegation (PEV) in Drosophila melanogaster. PLoS ONE. 2014;9:e71695 pubmed publisher
    ..These mutant alleles enhance both PDS at E1 and Position Effect Variegation (PEV) at w(m4), indicating a previously unknown common silencing mechanism between the two. ..
  2. Yang J, Smibert P, Westholm J, Jee D, Maurin T, Lai E. Intertwined pathways for Argonaute-mediated microRNA biogenesis in Drosophila. Nucleic Acids Res. 2014;42:1987-2002 pubmed publisher
    ..The difference was due to the 3' modification of single-stranded species in AGO2 by Hen1 methyltransferase, whose depletion permitted 3' trimming of Ago-cleaved pre-miRNAs in AGO2...
  3. Kolaczkowski B, Hupalo D, Kern A. Recurrent adaptation in RNA interference genes across the Drosophila phylogeny. Mol Biol Evol. 2011;28:1033-42 pubmed publisher
    ..Our observations suggest a predictive model of how selective pressures generated by evolutionary arms race scenarios may affect multiple genes across protein interaction networks and other biochemical pathways. ..
  4. Kawamura Y, Saito K, Kin T, Ono Y, Asai K, Sunohara T, et al. Drosophila endogenous small RNAs bind to Argonaute 2 in somatic cells. Nature. 2008;453:793-7 pubmed publisher
    ..Mutations in Dicer-2 caused an increase in retrotransposon transcripts. Together, our findings indicate that different types of small RNAs and Argonautes are used to repress retrotransposons in germline and somatic cells in Drosophila. ..
  5. Gomes A, Nolasco S, Soares H. Non-coding RNAs: multi-tasking molecules in the cell. Int J Mol Sci. 2013;14:16010-39 pubmed publisher
    ..We also highlighted the implications of biogenesis and gene expression deregulation of sncRNAs and lncRNAs in human diseases like cancer. ..
  6. Lee Y, Langley C. Long-term and short-term evolutionary impacts of transposable elements on Drosophila. Genetics. 2012;192:1411-32 pubmed publisher
  7. Olivieri D, Sykora M, Sachidanandam R, Mechtler K, Brennecke J. An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila. EMBO J. 2010;29:3301-17 pubmed publisher
    ..Loss of Zucchini leads to an accumulation of Piwi and Armitage in Yb bodies, indicating that Yb bodies are sites of primary piRNA biogenesis. ..
  8. Han B, Zamore P. piRNAs. Curr Biol. 2014;24:R730-3 pubmed publisher
  9. Soares Z, Gonçalves A, de Oliveira K, Marques J. Viral RNA recognition by the Drosophila small interfering RNA pathway. Microbes Infect. 2014;16:1013-21 pubmed publisher
    ..This antiviral response in fruit flies can help understand general principles of nucleic acid recognition. ..
  10. Wang H, Ma Z, Niu K, Xiao Y, Wu X, Pan C, et al. Antagonistic roles of Nibbler and Hen1 in modulating piRNA 3' ends in Drosophila. Development. 2016;143:530-9 pubmed publisher
    ..We show that there is a delicate balance in the interplay between Nbr and Hen1, a methyltransferase involved in 2'-O-methylation at the 3' terminal nucleotides of piRNAs, thus connecting two ..