gammaTub37C

Summary

Gene Symbol: gammaTub37C
Description: gamma-Tubulin at 37C
Alias: CG17566, D.m.GAMMA-37C, Dmel\CG17566, GTU88*, PL10, Rb1011, TW1, fs(2)TW1, fs(2)TWI, fsTW1, g-Tubulin, gamma Tub37CD, gamma tubulin, gamma-Tub, gamma-Tub37C, gamma-tub37CD, gamma-tubulin, gammaTUB, gammaTUB37C, gammaTub, gammaTub2, gammaTub37CD, gamma[mat], gammatub, 37CD gamma-tubulin, CG17566-PA, CG17566-PB, female sterile(2)Ted wright 1, gama-tubulin, gamma Tubulin2, gamma tubulin, gamma tubulin at 37C, gamma-Tubulin, gamma-tubulin (37C), gamma-tubulin-2, gamma-tubulin37C, gammaTub37C-PA, gammaTub37C-PB, gammatubulin, maternal gamma-tubulin, tubulin
Species: fruit fly

Top Publications

  1. ncbi Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome
    Y Zheng
    Department of Molecular Genetics, Ohio State University, Columbus 43210 1292
    Cell 65:817-23. 1991
  2. pmc Characterization of two related Drosophila gamma-tubulin complexes that differ in their ability to nucleate microtubules
    K Oegema
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 144:721-33. 1999
  3. pmc Fluorescence recovery kinetic analysis of gamma-tubulin binding to the mitotic spindle
    Mark A Hallen
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Biophys J 95:3048-58. 2008
  4. pmc A PAR-1-dependent orientation gradient of dynamic microtubules directs posterior cargo transport in the Drosophila oocyte
    Richard M Parton
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, England, UK
    J Cell Biol 194:121-35. 2011
  5. pmc Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cells
    Yasuhiko Terada
    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA
    J Cell Biol 162:757-63. 2003
  6. pmc The Drosophila pericentrin-like protein is essential for cilia/flagella function, but appears to be dispensable for mitosis
    Maruxa Martinez-Campos
    Wellcome Trust Cancer Research UK Gurdon Institute, Department of Genetics, University of Cambridge, England, UK
    J Cell Biol 165:673-83. 2004
  7. ncbi The fusome organizes the microtubule network during oocyte differentiation in Drosophila
    N C Grieder
    Howard Hughes Medical Institute, Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210, USA
    Development 127:4253-64. 2000
  8. pmc Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunits
    R N Gunawardane
    Howard Hughes Medical Institute, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
    J Cell Biol 151:1513-24. 2000
  9. ncbi The POLO kinase is required at multiple stages during spermatogenesis in Drosophila melanogaster
    S Herrmann
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, P 4150 Porto, Portugal
    Chromosoma 107:440-51. 1998
  10. ncbi Drosophila Lissencephaly-1 functions with Bic-D and dynein in oocyte determination and nuclear positioning
    A Swan
    Department of Biology, McGill University, 1205 Dr Penfield, Montreal, Quebec H3A 1B1, Canada
    Nat Cell Biol 1:444-9. 1999

Scientific Experts

  • P G Wilson
  • S A Endow
  • Yasuhiko Terada
  • M G Riparbelli
  • V E Foe
  • Yukiko Yamashita
  • Christiane Wiese
  • Gohta Goshima
  • C Gonzalez
  • Alistair P McGregor
  • Brigitte Raynaud-Messina
  • F Schnorrer
  • M Gho
  • Gaia Tavosanis
  • Nina Vogt
  • Fanni Gergely
  • M Moritz
  • T L Megraw
  • Elisabetta Bucciarelli
  • Jason Stumpff
  • Christel Verollet
  • Nathalie Colombié
  • S Chodagam
  • Richard M Parton
  • Judith L Leatherman
  • J W Raff
  • Ching Po Yang
  • Mark A Hallen
  • J G Wakefield
  • Dong Zhang
  • Jiuli Zhang
  • J R Huynh
  • Michel Wright
  • Jens Januschke
  • Maurizio Gatti
  • Henri Marc Bourbon
  • S Bonaccorsi
  • James Ashley
  • Teresa P Barros
  • Sandra Morales-Mulia
  • J K Jang
  • Jordan W Raff
  • Tin Tin Su
  • Chi Yun Pai
  • Maruxa Martinez-Campos
  • R D J Butcher
  • Shin Ichi Kawaguchi
  • Matthias G Fischer
  • D M Glover
  • Yixian Zheng
  • Christian F Lehner
  • Naoyuki Fuse
  • Cynthia Vied
  • Ruwanthi N Gunawardane
  • Fengwei Yu
  • Peter Deak
  • Byeong Jik Cha
  • D S Henderson
  • Alf Herzig
  • Daniela Berdnik
  • M J Lee
  • J Bolivar
  • M Schaefer
  • D St Johnston
  • G Callaini
  • J Ceron
  • C Vied
  • Hiroyuki Ohkura
  • Weiping Lu
  • Russell S Hamilton
  • Lei Yang
  • M Gatti
  • Y Zheng
  • Graeme Ball
  • Ilan Davis
  • C Fiona Cullen
  • K Afshar
  • Stephen Dinardo
  • N C Grieder
  • B M Lange
  • J A Kaltschmidt
  • A E Brent
  • R N Gunawardane
  • T T Su
  • V Barbosa
  • A Iwamatsu
  • O C Martin
  • Maria Patrizia Somma
  • Claudia Pellacani
  • Valeria Naim

Detail Information

Publications92

  1. ncbi Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome
    Y Zheng
    Department of Molecular Genetics, Ohio State University, Columbus 43210 1292
    Cell 65:817-23. 1991
    The mipA gene of A. nidulans encodes a newly discovered member of the tubulin superfamily of proteins, gamma-tubulin. In A...
  2. pmc Characterization of two related Drosophila gamma-tubulin complexes that differ in their ability to nucleate microtubules
    K Oegema
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 144:721-33. 1999
    gamma-tubulin exists in two related complexes in Drosophila embryo extracts (Moritz, M., Y. Zheng, B.M. Alberts, and K. Oegema. 1998. J. Cell Biol. 142:1- 12)...
  3. pmc Fluorescence recovery kinetic analysis of gamma-tubulin binding to the mitotic spindle
    Mark A Hallen
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Biophys J 95:3048-58. 2008
    ..Here we use it to analyze gamma-tubulin binding to the mitotic spindle and centrosomes to determine the role of gamma-tubulin in microtubule nucleation ..
  4. pmc A PAR-1-dependent orientation gradient of dynamic microtubules directs posterior cargo transport in the Drosophila oocyte
    Richard M Parton
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, England, UK
    J Cell Biol 194:121-35. 2011
    ..Our findings explain the biased random posterior cargo movements in the oocyte that establish the germline and posterior...
  5. pmc Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cells
    Yasuhiko Terada
    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA
    J Cell Biol 162:757-63. 2003
    ..and CNN are mutually dependent for localization at spindle poles, which is required for proper targeting of gamma-tubulin and other centrosomal components to the centrosome...
  6. pmc The Drosophila pericentrin-like protein is essential for cilia/flagella function, but appears to be dispensable for mitosis
    Maruxa Martinez-Campos
    Wellcome Trust Cancer Research UK Gurdon Institute, Department of Genetics, University of Cambridge, England, UK
    J Cell Biol 165:673-83. 2004
    ..Moreover, the flagella in mutant sperm are nonmotile. Thus, D-PLP is essential for the formation of functional cilia and flagella in flies...
  7. ncbi The fusome organizes the microtubule network during oocyte differentiation in Drosophila
    N C Grieder
    Howard Hughes Medical Institute, Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210, USA
    Development 127:4253-64. 2000
    ..During this same period, discrete foci rich in gamma tubulin that very probably correspond to migrating cystocyte centrosomes also associate with the fusome, first on the ..
  8. pmc Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunits
    R N Gunawardane
    Howard Hughes Medical Institute, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
    J Cell Biol 151:1513-24. 2000
    The gamma-tubulin ring complex (gammaTuRC) is important for microtubule nucleation from the centrosome...
  9. ncbi The POLO kinase is required at multiple stages during spermatogenesis in Drosophila melanogaster
    S Herrmann
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, P 4150 Porto, Portugal
    Chromosoma 107:440-51. 1998
    ..Immunostaining of polo mutant cells with alpha-tubulin shows several abnormalities of the meiotic spindle, including a significantly reduced central spindle...
  10. ncbi Drosophila Lissencephaly-1 functions with Bic-D and dynein in oocyte determination and nuclear positioning
    A Swan
    Department of Biology, McGill University, 1205 Dr Penfield, Montreal, Quebec H3A 1B1, Canada
    Nat Cell Biol 1:444-9. 1999
    ....
  11. ncbi The centrosome-nucleus complex and microtubule organization in the Drosophila oocyte
    Jens Januschke
    Institut Jacques Monod, Unité Mixte Recherche 7592, CNRS, Université Paris 6 et Paris 7, 2 place Jussieu, 75251 Paris Cedex 05, France
    Development 133:129-39. 2006
    ..Remarkably, the centrosomal components, gamma-tubulin and Drosophila pericentrin-like protein also strongly accumulate at the periphery of this nucleus...
  12. pmc The Drosophila gamma-tubulin small complex subunit Dgrip84 is required for structural and functional integrity of the spindle apparatus
    Nathalie Colombié
    Centre de Recherche en Pharmacologie Santé, Unité Mixte de Recherche 2587, Centre National de la Recherche Scientifique Pierre Fabre, Institut de Sciences et Technologies du Medicament de Toulouse, 31400 Toulouse, France
    Mol Biol Cell 17:272-82. 2006
    Gamma-tubulin, a protein critical for microtubule assembly, functions within multiprotein complexes. However, little is known about the respective role of gamma-tubulin partners in metazoans...
  13. pmc Essential role for gamma-tubulin in the acentriolar female meiotic spindle of Drosophila
    G Tavosanis
    European Molecular Biology Laboratory, Heidelberg, Germany
    EMBO J 16:1809-19. 1997
    ..In Drosophila melanogaster there are two gamma-tubulin genes, gammaTUB23C and gammaTUB37C. Here we report the cytological and molecular characterization of the 37C isoform...
  14. ncbi Maternally expressed gamma Tub37CD in Drosophila is differentially required for female meiosis and embryonic mitosis
    P G Wilson
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53705, USA
    Dev Biol 199:273-90. 1998
    We report functional analysis of gamma Tub37CD, a maternally synthesized gamma-tubulin that is highly expressed during oogenesis and utilized at centrosomes in precellular embryos...
  15. pmc Anastral spindle assembly and γ-tubulin in Drosophila oocytes
    Sharyn A Endow
    Department of Cell Biology and Structural Biology and Biophysics Program, Duke University, USA
    BMC Cell Biol 12:1. 2011
    ..It is still uncertain whether γ-tubulin, a microtubule nucleator essential for mitotic spindle assembly and maintenance, plays a role...
  16. doi Drosophila Dgt6 interacts with Ndc80, Msps/XMAP215, and gamma-tubulin to promote kinetochore-driven MT formation
    Elisabetta Bucciarelli
    Istituto Pasteur Fondazione Cenci Bolognetti and Istituto di Biologia e Patologia Molecolari del Consiglio Nazionale delle Ricerche CNR, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, P A Moro 5, 00185 Roma, Italy
    Curr Biol 19:1839-45. 2009
    ..Recent work has suggested that additional spindle MTs can be nucleated by gamma-tubulin ring complexes (gamma-TuRCs) that associate laterally with preexisting spindle MTs, leading to spindle ..
  17. ncbi Kinesin I-dependent cortical exclusion restricts pole plasm to the oocyte posterior
    Byeong Jik Cha
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Nat Cell Biol 4:592-8. 2002
    ....
  18. ncbi Differential expression of two gamma-tubulin isoforms during gametogenesis and development in Drosophila
    P G Wilson
    Department of Developmental Biology, Stanford University School of Medicine, California 94305, USA
    Dev Biol 184:207-21. 1997
    ..1991). We now report identification of a second gamma-tubulin gene, gamma Tub37CD. Immunoblot analysis and immunolocalization show that gamma Tub37CD and gamma Tub23C are differentially ..
  19. pmc The Drosophila wispy gene is required for RNA localization and other microtubule-based events of meiosis and early embryogenesis
    A E Brent
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Genetics 154:1649-62. 2000
    ..We propose that the wispy gene product functions directly in several microtubule-based events in meiosis and early embryogenesis and speculate about its possible mode of action...
  20. pmc The kinesinlike protein Subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes
    J K Jang
    Waksman Institute and Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 8020, USA
    Mol Biol Cell 16:4684-94. 2005
    ..We propose that Subito is required for establishing and/or maintaining the central spindle in Drosophila oocytes, and this substitutes for the role of centrosomes in organizing the bipolar spindle...
  21. ncbi PAR-1 is required for the maintenance of oocyte fate in Drosophila
    J R Huynh
    The Wellcome CRC Institute, University of Cambridge, Tennis Court Rd, Cambridge CB2 1QR, UK
    Development 128:1201-9. 2001
    ..Finally, we show that PAR-1 localises on the fusome, and provides a link between the asymmetry of the fusome and the selection of the oocyte...
  22. pmc The drosophila protein asp is involved in microtubule organization during spindle formation and cytokinesis
    J G Wakefield
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, 00185 Rome, Italy
    J Cell Biol 153:637-48. 2001
    ..both meiotic and mitotic divisions of asp mutants, microtubule nucleation occurs from the centrosome, and gamma-tubulin localizes correctly. However, spindle pole focusing and organization are severely affected...
  23. ncbi gamma-Tubulin is transiently associated with the Drosophila oocyte meiotic apparatus
    M G Riparbelli
    Department of Evolutionary Biology, University of Siena, Italy
    Eur J Cell Biol 75:21-8. 1998
    ..This center contained gamma-tubulin and CP190 antigens and nucleated a transient array of radial microtubules...
  24. pmc Proper recruitment of gamma-tubulin and D-TACC/Msps to embryonic Drosophila centrosomes requires Centrosomin Motif 1
    Jiuli Zhang
    Department of Pharmacology, The Cecil and Ida Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9051, USA
    Mol Biol Cell 18:4037-49. 2007
    ..Cnn Motif 1 is necessary for proper recruitment of gamma-tubulin, D-TACC (the homolog of vertebrate transforming acidic coiled-coil proteins [TACC]), and Minispindles (Msps) to ..
  25. pmc Aurora A activates D-TACC-Msps complexes exclusively at centrosomes to stabilize centrosomal microtubules
    Teresa P Barros
    The Wellcome Trust Cancer Research UK Gurdon Institute, Department of Genetics, Cambridge CB2 1QN, England, UK
    J Cell Biol 170:1039-46. 2005
    ..This may explain why centrosomes are such dominant sites of MT assembly during mitosis...
  26. ncbi Zygotic development without functional mitotic centrosomes
    T L Megraw
    Department of Biology and Howard Hughes Medical Institute, Indiana University, Bloomington, IN 47405, USA
    Curr Biol 11:116-20. 2001
    ..These results challenge long-held assumptions that the centrosome and the astral microtubules emanating from it are essential for development and are required specifically for spindle orientation during asymmetric cell divisions...
  27. pmc Three microtubule severing enzymes contribute to the "Pacman-flux" machinery that moves chromosomes
    Dong Zhang
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 177:231-42. 2007
    ..attached microtubule plus ends (Pacman) while being reeled in to spindle poles by the continual poleward flow of tubulin subunits driven by minus-end depolymerization (flux)...
  28. ncbi Centriole and centrosome dynamics during the embryonic cell cycles that follow the formation of the cellular blastoderm in Drosophila
    G Callaini
    Department of Evolutionary Biology, University of Siena, Italy
    Exp Cell Res 234:183-90. 1997
    ..The centrosomal material, as recognized by antibodies against CP190 and gamma-tubulin, does not show the typical shape changes observed in syncytial embryos, but remains compact throughout mitosis...
  29. ncbi Msps/XMAP215 interacts with the centrosomal protein D-TACC to regulate microtubule behaviour
    M J Lee
    Department of Genetics, Wellcome CRC Institute, Tennis Court Road, Cambridge CB2 1QR, UK
    Nat Cell Biol 3:643-9. 2001
    ..We propose that D-TACC and Msps normally cooperate to stabilize centrosomal microtubules by binding to their minus ends and binding to their plus ends as they grow out from the centrosome...
  30. ncbi Differential properties of the two Drosophila gamma-tubulin isotypes
    B Raynaud-Messina
    Institut de Pharmacologie et de Biologie Structurale, Centre National de la Recherche Scientifique, Toulouse France
    Eur J Cell Biol 80:643-9. 2001
    ..The two gamma-tubulins exhibit distinct biochemical properties. Gamma-tub23C is present in the soluble gamma-tubulin small complexes (10S) and gamma-tubulin big complexes (35S) and is loosely associated to the cytoskeleton...
  31. ncbi A requirement for the Abnormal Spindle protein to organise microtubules of the central spindle for cytokinesis in Drosophila
    Maria Giovanna Riparbelli
    University of Siena, Department of Evolutionary Biology, Via Mattioli 4, I 53100 Siena, Italy
    J Cell Sci 115:913-22. 2002
    ..Upon fertilisation, Asp is also recruited to the MTOC that nucleates the sperm aster. Asp is required for growth of the microtubules of the sperm aster, which in asp mutants remains diminutive and so prevents migration of the pronuclei...
  32. ncbi Drosophila Aurora-A is required for centrosome maturation and actin-dependent asymmetric protein localization during mitosis
    Daniela Berdnik
    Research Institute of Molecular Pathology I M P, Dr Bohr Gasse 7, A 1030, Vienna, Austria
    Curr Biol 12:640-7. 2002
    ..What triggers the asymmetric localization of Numb at the onset of mitosis is unclear...
  33. pmc Characterization of a Drosophila centrosome protein CP309 that shares homology with Kendrin and CG-NAP
    Shin Ichi Kawaguchi
    Department of Embryology, Carnegie Institution of Washington and Howard Hughes Medical Institute, Baltimore, Maryland 21210, USA
    Mol Biol Cell 15:37-45. 2004
    ..this novel Drosophila centrosome protein, centrosome protein of 309 kDa (CP309), cofractionates with the gamma-tubulin ring complex and the centrosome-complementing activity...
  34. pmc Spindle pole organization in Drosophila S2 cells by dynein, abnormal spindle protein (Asp), and KLP10A
    Sandra Morales-Mulia
    Section of Molecular and Cellular Biology, Center for Genetics and Development, University of California Davis, Davis, CA 95616, USA
    Mol Biol Cell 16:3176-86. 2005
    ..These results extend recent findings from Xenopus extracts to Drosophila cultured cells and suggest that common pathways contribute to spindle pole organization and length determination...
  35. ncbi Gamma-tubulin37C and gamma-tubulin ring complex protein 75 are essential for bicoid RNA localization during drosophila oogenesis
    Frank Schnorrer
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Genetik, Spemannstr 35, 72076, Tubingen, Germany
    Dev Cell 3:685-96. 2002
    ..They encode maternal gamma-Tubulin37C (gammaTub37C) and gamma-tubulin ring complex protein 75 (Dgrip75), both of which are gamma-tubulin ring complex components...
  36. pmc Characterization of a new gammaTuRC subunit with WD repeats
    Ruwanthi N Gunawardane
    Carnegie Institution of Washington Howard Hughes Medical Institute, Baltimore, Maryland 21210, USA
    Mol Biol Cell 14:1017-26. 2003
    The gamma-tubulin ring complex (gammaTuRC), consisting of multiple protein subunits, can nucleate microtubule assembly. Although many subunits of the gammaTuRC have been identified, a complete set remains to be defined in any organism...
  37. pmc Three-dimensional structural characterization of centrosomes from early Drosophila embryos
    M Moritz
    Department of Biochemistry and Biophysics, University of California at San Francisco 94143 0448, USA
    J Cell Biol 130:1149-59. 1995
    ....
  38. pmc Mutation of a Drosophila gamma tubulin ring complex subunit encoded by discs degenerate-4 differentially disrupts centrosomal protein localization
    V Barbosa
    Cancer Research Campaign, Cell Cycle Genetics Group, University of Cambridge, Department of Genetics, Cambridge, UK
    Genes Dev 14:3126-39. 2000
    We have cloned the Drosophila gene discs degenerate-4 (dd4) and find that it encodes a component of the gamma-tubulin ring complex (gammaTuRC) homologous to Spc98 of budding yeast...
  39. ncbi Centrosomes and microtubule organisation during Drosophila development
    C Gonzalez
    European Molecular Biology Laboratory Meyerhofstrasse 1, Germany
    J Cell Sci 111:2697-706. 1998
    ..In this review we present some of the many examples which illustrate how centrosomes and microtubule organisation changes through development in Drosophila and discuss some of its implications...
  40. ncbi Gamma-tubulin complexes: size does matter
    R Jeng
    Dept of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    Trends Cell Biol 9:339-42. 1999
    gamma-Tubulin is a conserved component of all microtubule-organizing centres and is required for these organelles to nucleate microtubule polymerization. However, the mechanism of nucleation is not known...
  41. ncbi The centrosomin protein is required for centrosome assembly and function during cleavage in Drosophila
    T L Megraw
    Department of Biology, Howard Hughes Medical Institute, Indiana University, Bloomington, IN 47405, USA
    Development 126:2829-39. 1999
    ..poles in the mutants contain little or no detectable amounts of the centrosomal proteins CP60, CP190 and (gamma)-tubulin and late stage embryos often do not have astral microtubules at their spindle poles...
  42. ncbi Abnormal spindle protein, Asp, and the integrity of mitotic centrosomal microtubule organizing centers
    M do Carmo Avides
    Cancer Research Campaign Cell Cycle Genetics Laboratories, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, UK
    Science 283:1733-5. 1999
    ..This was corrected by addition of purified Asp protein. Thus, Asp appears to hold together the microtubule-nucleating gamma-tubulin ring complexes that organize the mitotic centrosome.
  43. ncbi Functional analysis of the Drosophila diaphanous FH protein in early embryonic development
    K Afshar
    Department of Biology, Center for Molecular Genetics, University of California, San Diego, La Jolla CA 92093 0634, USA
    Development 127:1887-97. 2000
    ..Based on known biochemical functions of FH proteins, we propose that Diaphanous serves as a mediator between signaling molecules and actin organizers at specific phases of the cell cycle...
  44. pmc Recruitment of the gamma-tubulin ring complex to Drosophila salt-stripped centrosome scaffolds
    M Moritz
    Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143 0448, USA
    J Cell Biol 142:775-86. 1998
    ..2 M KI generates salt-resistant scaffolds that lack the centrosomal proteins CP190, CP60, centrosomin, and gamma-tubulin. To clarify the role of these proteins in microtubule nucleation by centrosomes and to identify additional ..
  45. ncbi Failure of pronuclear migration and repeated divisions of polar body nuclei associated with MTOC defects in polo eggs of Drosophila
    M G Riparbelli
    University of Siena, Department of Evolutionary Biology, Via Mattioli 4, I 53100 Siena, Italy
    J Cell Sci 113:3341-50. 2000
    ..Migration of the female pronucleus did not take place and so a gonomeric spindle could not form. We discuss these findings in relation to the known roles of polo like kinases in regulating the behaviour of MTOCs...
  46. ncbi Spindle assembly in Drosophila neuroblasts and ganglion mother cells
    S Bonaccorsi
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, P le Aldo Moro 5, 00185 Rome, Italy
    Nat Cell Biol 2:54-6. 2000
  47. pmc Drosophila polo kinase is required for cytokinesis
    M Carmena
    CRC Cell Cycle Group, Cancer Research Campaign Laboratories, Department of Anatomy and Physiology, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, Scotland
    J Cell Biol 143:659-71. 1998
    ..We discuss these findings in respect to conserved functions for the Polo-like kinases in regulating progression through M phase, including the earliest events of cytokinesis...
  48. pmc Cytoplasmic dynein is required for the nuclear attachment and migration of centrosomes during mitosis in Drosophila
    J T Robinson
    University of Minnesota, Department of Genetics, Cell Biology and Development, St Paul, Minnesota 55108 1095, USA
    J Cell Biol 146:597-608. 1999
    ..The disruption of these centrosome attachments in mutant embryos reveals a critical role for dynein function and centrosome positioning in the spatial organization of the syncytial cytoplasm of the developing embryo...
  49. ncbi Assembly and dynamics of an anastral:astral spindle: the meiosis II spindle of Drosophila oocytes
    S A Endow
    Department of Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    J Cell Sci 111:2487-95. 1998
    ..gamma-Tubulin transiently localizes to the central spindle pole body, implying that the body acts as a microtubule nucleating ..
  50. pmc Asymmetric inheritance of mother versus daughter centrosome in stem cell division
    Yukiko M Yamashita
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305 5329, USA
    Science 315:518-21. 2007
    ..The mother centrosome remains anchored near the niche while the daughter centrosome migrates to the opposite side of the cell before spindle formation...
  51. ncbi Structure of the gamma-tubulin ring complex: a template for microtubule nucleation
    M Moritz
    Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, 513 Parnassus Avenue, San Francisco, California 94143, USA
    Nat Cell Biol 2:365-70. 2000
    The gamma-tubulin ring complex (gammaTuRC) is a protein complex of relative molecular mass approximately 2.2 x 10(6) that nucleates microtubules at the centrosome...
  52. ncbi Centrosome migration into the Drosophila oocyte is independent of BicD and egl, and of the organisation of the microtubule cytoskeleton
    J Bolivar
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Development 128:1889-97. 2001
    ..Thus, centrosome migration is independent of the organisation of the microtubule cytoskeleton, and seems to depend instead on the polarity of the fusome...
  53. ncbi The gammaTuRC components Grip75 and Grip128 have an essential microtubule-anchoring function in the Drosophila germline
    Nina Vogt
    Max Planck Institute for Developmental Biology, Department of Genetics, Spemannstr 35, 72076 Tubingen, Germany
    Development 133:3963-72. 2006
    The gamma-tubulin ring complex (gammaTuRC) forms an essential template for microtubule nucleation in animal cells...
  54. ncbi Elongation of centriolar microtubule triplets contributes to the formation of the mitotic spindle in gamma-tubulin-depleted cells
    Brigitte Raynaud-Messina
    ISTMT, Centre de Recherche en Pharmacologie Santé, UMR 2587 CNRS P Fabre, 3 rue des Satellites, 31 400 Toulouse, France
    J Cell Sci 117:5497-507. 2004
    The assembly of the mitotic spindle after depletion of the major gamma-tubulin isotype by RNA-mediated interference was assessed in the Drosophila S2 cell line...
  55. pmc Germline self-renewal requires cyst stem cells and stat regulates niche adhesion in Drosophila testes
    Judith L Leatherman
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine Institute for Regenerative Medicine, University of Pennsylvania Philadelphia, USA
    Nat Cell Biol 12:806-11. 2010
    ..This work also reconciles key differences in GSC renewal between Drosophila testis and ovary niches, and highlights how a niche can coordinate the production of distinct lineages by having one stem cell type rely on a second...
  56. pmc Drosophila Wee1 kinase regulates Cdk1 and mitotic entry during embryogenesis
    Jason Stumpff
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Curr Biol 14:2143-8. 2004
    ..These findings modify previous models about cell cycle regulation in syncytial embryos and demonstrate that Wee1 kinases can regulate mitotic entry in vivo during metazoan development even in cycles that lack a G2 phase...
  57. ncbi The mitotic arrest in response to hypoxia and of polar bodies during early embryogenesis requires Drosophila Mps1
    Matthias G Fischer
    BZMB, Department of Genetics, University of Bayreuth, 94440 Bayreuth, Germany
    Curr Biol 14:2019-24. 2004
    ..Finally, we show that Mps1 and the mitotic spindle checkpoint are responsible for the developmental cell cycle arrest of the three haploid products of female meiosis that are not used as the female pronucleus...
  58. ncbi The centrosomal protein CP190 is a component of the gypsy chromatin insulator
    Chi Yun Pai
    Department of Biology, Johns Hopkins University, 3400 N Charles Street, Baltimore, Maryland 21218, USA
    Mol Cell 16:737-48. 2004
    ..The results suggest that endogenous gypsy insulators contain binding sites for CP190, which is essential for insulator function, and may or may not contain binding sites for Su(Hw) and Mod(mdg4)2.2...
  59. doi Drosophila mars is required for organizing kinetochore microtubules during mitosis
    Ching Po Yang
    Department of Life Science, Tunghai University, Taichung, Taiwan, ROC
    Exp Cell Res 314:3209-20. 2008
    ..In summary, this study has extended our previous study of Mars in cell cycle regulation and provided further evidence showing that Mars is required for the assembly of kinetochore microtubules...
  60. pmc Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle
    Gohta Goshima
    Institute for Advanced Research, Nagoya University, Chikusa ku, Nagoya 464 8601, Japan
    J Cell Biol 181:421-9. 2008
    Since the discovery of gamma-tubulin, attention has focused on its involvement as a microtubule nucleator at the centrosome...
  61. ncbi The centrosomal protein CP190 regulates myosin function during early Drosophila development
    S Chodagam
    The Gurdon Institute, Department of Genetics, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Curr Biol 15:1308-13. 2005
    ..Thus, CP190 has distinct nuclear and centrosomal functions, and it provides a crucial link between the centrosome/MT and actin/myosin cytoskeletal systems in early embryos...
  62. ncbi Centrosome inheritance in the male germ line of Drosophila requires hu-li tai-shao function
    Patricia G Wilson
    Department of Biology, Georgia State University, MSC 8L0389, 33, Gilmer Street SE, Unit 8, Atlanta, GA 30303 3088, USA
    Cell Biol Int 29:360-9. 2005
    ..Collectively, these results indicate that hts function is necessary for centrosome inheritance in spermatocytes as well as for male fertility...
  63. ncbi Centrosomal TACCtics
    Fanni Gergely
    Wellcome CR UK Institute, Department of Pharmacology, University of Cambridge, UK
    Bioessays 24:915-25. 2002
    ..In this review, I summarise what we already know of this protein family and will use the TACC proteins to illustrate the many facets that centrosomes have developed during the course of evolution...
  64. ncbi A mutation (dosach) in Drosophila which affects aster formation and nuclear migration during cleavage
    S S Craig
    School of Biological Sciences, Flinders University of South Australia, Australia
    Biol Cell 87:45-54. 1996
    ..Confocal microscopy has been used to correlate organization of microtubules, centrosomal proteins and chromosomes in wild-type and dosach (dos) embryos...
  65. pmc Spindle assembly and cytokinesis in the absence of chromosomes during Drosophila male meiosis
    Elisabetta Bucciarelli
    Istituto Pasteur Fondazione Cenci Bolognetti, 00185 Rome, Italy
    J Cell Biol 160:993-9. 2003
    ..This suggests that the association of Aurora B with chromosomes is not a prerequisite for its accumulation at the central spindle, or for its function during cytokinesis...
  66. ncbi Heterotrimeric G proteins regulate daughter cell size asymmetry in Drosophila neuroblast divisions
    Naoyuki Fuse
    Laboratory for Cell Asymmetry, Center for Developmental Biology, RIKEN, Chuou ku, Kobe 650 0047, Japan
    Curr Biol 13:947-54. 2003
    ..Furthermore, the multiple equal cleavages of G beta mutant neuroblasts accompany neural defects; this finding suggests indispensable roles of eccentric division in assuring the stem cell properties of neuroblasts...
  67. ncbi Effects of Ddc cluster lethal alleles on ovary growth, attachment, and egg production in Drosophila
    E McCrady
    Department of Biology, University of North Carolina at Greensboro 27412
    J Exp Zool 268:469-76. 1994
    ..Heteroallelic heterozygotes of Bd7 x Bd4 also produced fully fertile ovaries, but no other heteroallelic combinations did so.(ABSTRACT TRUNCATED AT 250 WORDS)..
  68. ncbi Polar organization of gamma-tubulin in acentriolar mitotic spindles of Drosophila melanogaster cells
    A Debec
    Groupe de Génétique Cellulaire et Moléculaire, Unité Associée du CNRS 1135, Universite Paris 6, France
    J Cell Sci 108:2645-53. 1995
    The spindle pole localization of gamma-tubulin was compared in wild type and acentriolar cultured Drosophila cells using polyclonal antibodies specifically raised against the carboxy terminal amino acid sequence of Drosophila gamma-..
  69. ncbi Molecular cloning of linotte in Drosophila: a novel gene that functions in adults during associative learning
    G M Bolwig
    Cold Spring Harbor Laboratory, New York 11724, USA
    Neuron 15:829-42. 1995
    ..The lio gene produces only one transcript, the level of expression of which varies throughout development. Sequence analysis indicates that lio encodes a novel protein...
  70. ncbi Heterotrimeric G proteins direct two modes of asymmetric cell division in the Drosophila nervous system
    M Schaefer
    Research Institute of Molecular Pathology I M P, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Cell 107:183-94. 2001
    ..Thus, asymmetric activation of heterotrimeric G proteins by a receptor-independent mechanism may orient asymmetric cell divisions in different cell types...
  71. ncbi DNA defects target the centrosome
    T T Su
    Nat Cell Biol 2:E28-9. 2000
    ..These defects may result from the workings of a surveillance mechanism that acts to cull irreparable nuclei...
  72. ncbi Rotation and asymmetry of the mitotic spindle direct asymmetric cell division in the developing central nervous system
    J A Kaltschmidt
    Wellcome CRC Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Nat Cell Biol 2:7-12. 2000
    ..This observation contradicts the widely held hypothesis that the cleavage furrow is always placed midway between the two centrosomes...
  73. ncbi Cytological characterisation of the mutant phenotypes produced during early embryogenesis by null and loss-of-function alleles of the gammaTub37C gene in Drosophila
    S Llamazares
    Cell Biology Programme, EMBL, Meyerhofstr, Heidelberg, Germany
    J Cell Sci 112:659-67. 1999
    We have studied the mutant phenotypes brought about during early embryogenesis by mutation in the gammaTub37C gene, one of the two isoforms of gamma-tubulin that have been identified in Drosophila...
  74. ncbi Protein phosphatase 4 is an essential enzyme required for organisation of microtubules at centrosomes in Drosophila embryos
    N R Helps
    Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland, UK
    J Cell Sci 111:1331-40. 1998
    ..A reduction in the staining of gamma-tubulin at centrosomes in cmm embryos suggests a conformational change or relocation of this protein, which is known to ..
  75. ncbi The Wilhelmine E. Key 1992 Invitational lecture. Phenotypic analysis of the Dopa decarboxylase gene cluster mutants in Drosophila melanogaster
    T R Wright
    University of Virginia, Department of Biology, Charlottesville 22903 2477, USA
    J Hered 87:175-90. 1996
    ..Thus, the Ddc gene cluster represents in higher eukaryotes an unusual example of a large cluster of functionally related genes involved in a common physiological process...
  76. ncbi Mutations in mákos, a Drosophila gene encoding the Cdc27 subunit of the anaphase promoting complex, enhance centrosomal defects in polo and are suppressed by mutations in twins/aar, which encodes a regulatory subunit of PP2A
    Peter Deak
    Cancer Research UK, Cell Cycle Genetics Research Group, University of Cambridge, Department of Genetics, Downing Street, Cambridge CB2 3EH, UK
    J Cell Sci 116:4147-58. 2003
    ..the reduced ability of hypomorphic polo mutant alleles to recruit and/or maintain the centrosomal antigens gamma-tubulin and CP190 at the spindle poles...
  77. pmc The genetic and molecular organization of the Dopa decarboxylase gene cluster of Drosophila melanogaster
    D G Stathakis
    Department of Biology, University of Virginia, Charlottesville 22903 2477, USA
    Genetics 141:629-55. 1995
    ....
  78. ncbi Control of neuronal pathway selection by a Drosophila receptor protein-tyrosine kinase family member
    C A Callahan
    Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, San Diego, California 92186, USA
    Nature 376:171-4. 1995
    ..In drl mutant embryos these neurons fail to make the correct pathway choices. Our results provide evidence for receptor protein-tyrosine kinase involvement in key aspects of neuronal pathway recognition...
  79. pmc Hsp90 is a core centrosomal component and is required at different stages of the centrosome cycle in Drosophila and vertebrates
    B M Lange
    Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    EMBO J 19:1252-62. 2000
    ..This suggests that another role of Hsp90 might be to ensure proper centrosome function...
  80. pmc Mechanisms for focusing mitotic spindle poles by minus end-directed motor proteins
    Gohta Goshima
    The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94107, USA
    J Cell Biol 171:229-40. 2005
    ..From these results and simulations, we propose a model on how two minus end-directed motors cooperate to ensure spindle pole coalescence during mitosis...
  81. ncbi The Drosophila centrosome-associated protein CP190 is essential for viability but not for cell division
    R D J Butcher
    NERC Center for Population Biology, Imperial College London, Silwood Park Campus, Ascot SL5 7PY, UK
    J Cell Sci 117:1191-9. 2004
    ..Thus, CP190 plays an essential role in flies that is independent of its association with centrosomes or microtubules...
  82. pmc Distinct roles of Galphai and Gbeta13F subunits of the heterotrimeric G protein complex in the mediation of Drosophila neuroblast asymmetric divisions
    Fengwei Yu
    MRC Centre for Developmental Neurobiology, 4th Fl, New Hunts House, Guy s Campus, King s College London, London SE1 1UL, UK
    J Cell Biol 162:623-33. 2003
    ..Importantly, our results have also revealed a novel aspect of apical complex function, that is, the two apical pathways act redundantly to suppress the formation of basal astral microtubules in neuroblasts...
  83. ncbi The abnormal spindle protein is required for germ cell mitosis and oocyte differentiation during Drosophila oogenesis
    Maria Giovanna Riparbelli
    Department of Evolutionary Biology, University of Siena, I 53100, Italy
    Exp Cell Res 298:96-106. 2004
    ..spindles of the mutant cystocytes are composed by wavy microtubules and have abnormal poles that often lack gamma-tubulin. The fusome structure is also compromised...
  84. pmc gamma-Tubulin function during female germ-cell development and oogenesis in Drosophila
    Gaia Tavosanis
    Cell Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Proc Natl Acad Sci U S A 100:10263-8. 2003
    ..To gain a better understanding of their molecular nature, we have studied the centrosomal component gamma-tubulin during Drosophila oogenesis...
  85. ncbi Antizyme is a target of sex-lethal in the Drosophila germline and appears to act downstream of hedgehog to regulate sex-lethal and cyclin B
    Cynthia Vied
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA
    Dev Biol 253:214-29. 2003
    ..This regulation of Sex-lethal by gutfeeling appears to occur downstream of the Hedgehog signal. We also show that Hedgehog, Gutfeeling, and Sex-lethal function to regulate Cyclin B, providing a link between Sex-lethal and mitosis...
  86. ncbi Fasciclin II signals new synapse formation through amyloid precursor protein and the scaffolding protein dX11/Mint
    James Ashley
    Department of Neurobiology, University of Massachusetts Medical School, Worcester, Massachusetts 01605 2324, USA
    J Neurosci 25:5943-55. 2005
    ..These results provide a novel mechanism by which cell adhesion molecules are regulated and provide fresh insights into the normal operation of APP during synapse development...
  87. pmc Proteolytic cleavage of the THR subunit during anaphase limits Drosophila separase function
    Alf Herzig
    Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany
    Genes Dev 16:2443-54. 2002
    ....
  88. ncbi The sex determination master switch, Sex-lethal, responds to Hedgehog signaling in the Drosophila germline
    C Vied
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, Alabama 35294, USA
    Development 128:2649-60. 2001
    ..This is the first demonstration that downstream components of the Hedgehog signaling pathway regulate a target other than Cubitus interruptus...
  89. pmc Drosophila Wee1 interacts with members of the gammaTURC and is required for proper mitotic-spindle morphogenesis and positioning
    Jason Stumpff
    Department of Molecular, Cellular, and Developmental Biology, 347 UCB, University of Colorado, Boulder Boulder, Colorado 80309, USA
    Curr Biol 15:1525-34. 2005
    ..Wee1 kinases delay entry into mitosis by phosphorylating and inactivating cyclin-dependent kinase 1 (Cdk1). Loss of this activity in many systems, including Drosophila, leads to premature mitotic entry...
  90. ncbi Patterns of cell division and expression of asymmetric cell fate determinants in postembryonic neuroblast lineages of Drosophila
    J Ceron
    Instituto de Neurociencias, Universidad Miguel Hernandez y CSIC, Alicante, 03550, Spain
    Dev Biol 230:125-38. 2001
    ..vi) Prospero is transiently expressed in one of the two sibling GCs generated by the division of GMCs. The implications of these results on cell fate specification and differentiation of adult brain neurons are discussed...
  91. ncbi Microtubules and mitotic cycle phase modulate spatiotemporal distributions of F-actin and myosin II in Drosophila syncytial blastoderm embryos
    V E Foe
    Department of Zoology, University of Washington, Seattle WA 98195 1800, USA
    Development 127:1767-87. 2000
    ....
  92. pmc Drosophila melanogaster gamma-TuRC is dispensable for targeting gamma-tubulin to the centrosome and microtubule nucleation
    Christel Verollet
    Centre de Recherche en Pharmacologie, santé, UMR 2587, Centre National de la Recherche Scientifique Pierre Fabre, Institut de Sciences et Technologies du Medicament de Toulouse, 31432 Toulouse, Cedex 4, France
    J Cell Biol 172:517-28. 2006
    In metazoans, gamma-tubulin acts within two main complexes, gamma-tubulin small complexes (gamma-TuSCs) and gamma-tubulin ring complexes (gamma-TuRCs)...

Research Grants4

  1. Physiological regulation and function of asymmetric stem cell division
    Yukiko Yamashita; Fiscal Year: 2009
    ..Understanding of such mechanisms may allow us to manipulate stem cell behavior; for example, normal stem cells can be expanded in culture for transplantation, or we can inhibit the expansion of cancerous stem cells. ..
  2. Physiological regulation and function of asymmetric stem cell division
    Yukiko Yamashita; Fiscal Year: 2010
    ..Understanding of such mechanisms may allow us to manipulate stem cell behavior;for example, normal stem cells can be expanded in culture for transplantation, or we can inhibit the expansion of cancerous stem cells. ..
  3. Physiological regulation and function of asymmetric stem cell division
    Yukiko Yamashita; Fiscal Year: 2009
    ..Understanding of such mechanisms may allow us to manipulate stem cell behavior; for example, normal stem cells can be expanded in culture for transplantation, or we can inhibit the expansion of cancerous stem cells. ..
  4. Physiological regulation and function of asymmetric stem cell division
    Yukiko Yamashita; Fiscal Year: 2011
    ..Understanding of such mechanisms may allow us to manipulate stem cell behavior;for example, normal stem cells can be expanded in culture for transplantation, or we can inhibit the expansion of cancerous stem cells. ..