Gene Symbol: Eps-15
Description: Epidermal growth factor receptor pathway substrate clone 15
Alias: CG16932, Dmel\CG16932, EH1, EPS-15, EPS15, Eps15, anon-WO0118547.85, eps-15, eps15, i31, epidermal growth factor receptor pathway substrate clone 15, CG16932-PA, CG16932-PB, CG16932-PC, CG16932-PD, Eps-15-PA, Eps-15-PB, Eps-15-PC, Eps-15-PD, Eps15 homologue 1
Species: fruit fly

Top Publications

  1. Majumdar A, Ramagiri S, Rikhy R. Drosophila homologue of Eps15 is essential for synaptic vesicle recycling. Exp Cell Res. 2006;312:2288-98 pubmed
    The mammalian protein Eps15 is phosphorylated by EGF receptor tyrosine kinase and has been shown to interact with several components of the endocytic machinery...
  2. Tjota M, Lee S, Wu J, Williams J, Khanna M, Thomas G. Annexin B9 binds to ?(H)-spectrin and is required for multivesicular body function in Drosophila. J Cell Sci. 2011;124:2914-26 pubmed publisher
    ..in basolateral ?(H) and its appearance on cytoplasmic vesicles that overlap with the MVB markers Hrs, Vps16 and EPS15. Similar colocalizations are seen when ?(H)-positive endosomes are generated either by upregulation of ?(H) in pak ..
  3. Venken K, He Y, Hoskins R, Bellen H. P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogaster. Science. 2006;314:1747-51 pubmed
    ..This transgenesis platform should greatly facilitate structure/function analyses of most Drosophila genes...
  4. Koh T, Korolchuk V, Wairkar Y, Jiao W, Evergren E, Pan H, et al. Eps15 and Dap160 control synaptic vesicle membrane retrieval and synapse development. J Cell Biol. 2007;178:309-22 pubmed
    b>Epidermal growth factor receptor pathway substrate clone 15 (Eps15) is a protein implicated in endocytosis, endosomal protein sorting, and cytoskeletal organization...
  5. Tang H, Rompani S, Atkins J, Zhou Y, Osterwalder T, Zhong W. Numb proteins specify asymmetric cell fates via an endocytosis- and proteasome-independent pathway. Mol Cell Biol. 2005;25:2899-909 pubmed
    ..Here we show that two Drosophila EH proteins, Eps15 homologue 1 (EH1) and the dynamin-associated 160-kDa protein (Dap160), negatively regulate Notch signaling...
  6. Chen C, Buhl E, Xu M, Croset V, Rees J, Lilley K, et al. Drosophila Ionotropic Receptor 25a mediates circadian clock resetting by temperature. Nature. 2015;527:516-20 pubmed publisher
    ..This pathway operates in the absence of known 'hot' and 'cold' sensors in the Drosophila antenna, revealing the existence of novel periphery-to-brain temperature signalling channels. ..
  7. Halder P, Chen Y, Brauckhoff J, Hofbauer A, Dabauvalle M, Lewandrowski U, et al. Identification of Eps15 as antigen recognized by the monoclonal antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila brain. PLoS ONE. 2011;6:e29352 pubmed publisher
    ..Analysis of a mutant of a candidate gene identified the Drosophila homolog of the Epidermal growth factor receptor Pathway Substrate clone 15 (Eps15) as the antigen for these two antibodies.
  8. Miller K. Extending the Arp2/3 complex and its regulation beyond the leading edge. J Cell Biol. 2002;156:591-3 pubmed
    ..These studies also show that assembly of other actin structures is independent of these proteins, suggesting that alternative mechanisms also exist. ..
  9. Kasprowicz J, Kuenen S, Miskiewicz K, Habets R, Smitz L, Verstreken P. Inactivation of clathrin heavy chain inhibits synaptic recycling but allows bulk membrane uptake. J Cell Biol. 2008;182:1007-16 pubmed publisher
    ..Our data not only indicate that chc is critical for synaptic vesicle recycling but they also show that in the absence of the protein, bulk retrieval mediates massive synaptic membrane internalization. ..

More Information


  1. Winther Ã, Vorontsova O, Rees K, Näreoja T, Sopova E, Jiao W, et al. An Endocytic Scaffolding Protein together with Synapsin Regulates Synaptic Vesicle Clustering in the Drosophila Neuromuscular Junction. J Neurosci. 2015;35:14756-70 pubmed publisher
    ..We also identify the endocytic scaffolding complex that includes Dap160 as a regulator of the events linking exocytosis and endocytosis in synapses. ..
  2. Nahm M, Lee M, Parkinson W, Lee M, Kim H, Kim Y, et al. Spartin regulates synaptic growth and neuronal survival by inhibiting BMP-mediated microtubule stabilization. Neuron. 2013;77:680-95 pubmed publisher
    ..We have generated a Drosophila disease model showing that Spartin functions presynaptically with endocytic adaptor Eps15 to regulate synaptic growth and function...
  3. Matta S, Van Kolen K, da Cunha R, van den Bogaart G, Mandemakers W, Miskiewicz K, et al. LRRK2 controls an EndoA phosphorylation cycle in synaptic endocytosis. Neuron. 2012;75:1008-21 pubmed publisher
  4. Wang P, Pai L. D-Cbl binding to Drk leads to dose-dependent down-regulation of EGFR signaling and increases receptor-ligand endocytosis. PLoS ONE. 2011;6:e17097 pubmed publisher
    ..The fusion proteins we developed may provide alternative strategies for therapy of cancers caused by hyper-activation of EGFR signaling. ..
  5. Jafar Nejad H, Norga K, Bellen H. Numb: "Adapting" notch for endocytosis. Dev Cell. 2002;3:155-6 pubmed
    ..In this issue of Developmental Cell, Berdnik et al. show that Numb recruits alpha-Adaptin and that this physical interaction plays a role in downregulating Notch, presumably by stimulating endocytosis of Notch. ..
  6. Xie X, Cho B, Fischer J. Drosophila Epsin's role in Notch ligand cells requires three Epsin protein functions: the lipid binding function of the ENTH domain, a single Ubiquitin interaction motif, and a subset of the C-terminal protein binding modules. Dev Biol. 2012;363:399-412 pubmed publisher
    ..We propose that Epsin's specificity for Notch signaling simply reflects its unique ability to interact with the plasma membrane, Ubiquitin, and proteins that bind Clathrin. ..
  7. Giagtzoglou N, Lin Y, Haueter C, Bellen H. Importin 13 regulates neurotransmitter release at the Drosophila neuromuscular junction. J Neurosci. 2009;29:5628-39 pubmed publisher
    ..Interestingly, imp13 is required in the muscles to control presynaptic release. Thus, imp13 is a novel factor that affects neurotransmitter release at the fly NMJ. Its role in the context of synaptic homeostasis is discussed. ..
  8. Peng Y, Yang W, Lin W, Lai T, Chien C. Nak regulates Dlg basal localization in Drosophila salivary gland cells. Biochem Biophys Res Commun. 2009;382:108-13 pubmed publisher
    ..Therefore, Nak regulates the transition of Dlg distribution likely through endocytosis of Dlg from the apical membrane domain and transcytosis of Dlg to the basolateral membrane domain during the maturation of SGs development. ..
  9. Crosetto N, Tikkanen R, Dikic I. Oncogenic breakdowns in endocytic adaptor proteins. FEBS Lett. 2005;579:3231-8 pubmed
    ..We discuss how breakdowns in the function of endocytic adaptors might facilitate impairment of tissue homeostasis and consequent tumor development. ..
  10. Vijayakrishnan N, Phillips S, Broadie K. Drosophila rolling blackout displays lipase domain-dependent and -independent endocytic functions downstream of dynamin. Traffic. 2010;11:1567-78 pubmed publisher
    ..We conclude that RBO possesses both lipase domain-dependent and scaffolding functions with differential requirements in non-neuronal versus neuronal endocytosis mechanisms downstream of dynamin GTPase activity. ..
  11. Jha A, Watkins S, Traub L. The apoptotic engulfment protein Ced-6 participates in clathrin-mediated yolk uptake in Drosophila egg chambers. Mol Biol Cell. 2012;23:1742-64 pubmed publisher
  12. Li X, Kuromi H, Briggs L, Green D, Rocha J, Sweeney S, et al. Bicaudal-D binds clathrin heavy chain to promote its transport and augments synaptic vesicle recycling. EMBO J. 2010;29:992-1006 pubmed publisher
    ..Our results implicate BicD as a new player in clathrin-associated trafficking processes and show a novel requirement for microtubule-based motor transport in the synaptic vesicle cycle. ..
  13. Wu J, Bakerink K, Evangelista M, Thomas G. Cytoplasmic capes are nuclear envelope intrusions that are enriched in endosomal proteins and depend upon ?H-spectrin and Annexin B9. PLoS ONE. 2014;9:e93680 pubmed publisher
    ..Diminution of these structures does not lead to a change in mSpitz processing. These results extend the endosomal influence of ?H and its partner Annexin B9 to this unusual compartment at the nuclear envelope. ..
  14. Li M, Lai P, Chou Y, Chi A, Mi Y, Khoo K, et al. Protein tyrosine phosphatase PTPN3 inhibits lung cancer cell proliferation and migration by promoting EGFR endocytic degradation. Oncogene. 2015;34:3791-803 pubmed publisher
    ..We further identified EGFR pathway substrate 15 (Eps15) as a target of dPtpmeg and its human homolog PTPN3...
  15. Hamanaka Y, Meinertzhagen I. Immunocytochemical localization of synaptic proteins to photoreceptor synapses of Drosophila melanogaster. J Comp Neurol. 2010;518:1133-55 pubmed publisher
    ..We used monoclonal antibodies against Bruchpilot, epidermal growth factor receptor pathway substrate clone 15 (EPS-15), and cysteine string protein (CSP), all raised against a fly head homogenate, ..