Gene Symbol: E2f2
Description: E2F transcription factor 2
Alias: CG1071, Dmel\CG1071, E2F, E2F2, E2f, dE2F-2, dE2F2, dE2f2, de2f2, e2f2, E2F transcription factor 2, CG1071-PA, CG1071-PB, E2f2-PA, E2f2-PB
Species: fruit fly
Products:     E2f2

Top Publications

  1. Frolov M, Stevaux O, Moon N, Dimova D, Kwon E, Morris E, et al. G1 cyclin-dependent kinases are insufficient to reverse dE2F2-mediated repression. Genes Dev. 2003;17:723-8 pubmed
    Here we show that the cell cycle defects of dE2F1-depleted cells depend on the cooperative effects of dE2F2 and DACAPO (DAP), an inhibitor of Cyclin E/cyclin-dependent kinase 2 (CycE/cdk2)...
  2. Frolov M, Moon N, Dyson N. dDP is needed for normal cell proliferation. Mol Cell Biol. 2005;25:3027-39 pubmed
    ..Our results show that dDP requires dE2F1 and dE2F2 for DNA-binding activity in vitro and in vivo...
  3. Sim C, Perry S, Tharadra S, Lipsick J, Ray A. Epigenetic regulation of olfactory receptor gene expression by the Myb-MuvB/dREAM complex. Genes Dev. 2012;26:2483-98 pubmed publisher
    ..Conversely, other complex members, Mip120 (Myb-interacting protein 120) and E2F2, are required for repression of Gr63a in inappropriate neurons...
  4. Sawado T, Yamaguchi M, Nishimoto Y, Ohno K, Sakaguchi K, Matsukage A. dE2F2, a novel E2F-family transcription factor in Drosophila melanogaster. Biochem Biophys Res Commun. 1998;251:409-15 pubmed
    ..We have now identified and characterized a cDNA encoding another E2F family member in Drosophila, termed dE2F2. The predicted amino acid sequence shares 38...
  5. Korenjak M, Taylor Harding B, Binné U, Satterlee J, Stevaux O, Aasland R, et al. Native E2F/RBF complexes contain Myb-interacting proteins and repress transcription of developmentally controlled E2F target genes. Cell. 2004;119:181-93 pubmed
    The retinoblastoma tumor suppressor protein (pRb) regulates gene transcription by binding E2F transcription factors...
  6. Ambrus A, Nicolay B, Rasheva V, Suckling R, Frolov M. dE2F2-independent rescue of proliferation in cells lacking an activator dE2F1. Mol Cell Biol. 2007;27:8561-70 pubmed
    ..To date, the only known way to rescue the proliferation block in de2f1 mutants was through the inactivation of dE2F2. This suggests that dE2F2 provides a major contribution to the de2f1 mutant phenotype...
  7. Stevaux O, Dimova D, Frolov M, Taylor Harding B, Morris E, Dyson N. Distinct mechanisms of E2F regulation by Drosophila RBF1 and RBF2. EMBO J. 2002;21:4927-37 pubmed
    ..In contrast, RBF2 binds exclusively to dE2F2, a form of E2F that functions as a transcriptional repressor. We find that RBF2-mediated repression requires dE2F2...
  8. Longworth M, Herr A, Ji J, Dyson N. RBF1 promotes chromatin condensation through a conserved interaction with the Condensin II protein dCAP-D3. Genes Dev. 2008;22:1011-24 pubmed publisher
    ..homologs (pRB, p130, and p107) are best known for their regulation of the G1/S transition via the repression of E2F-dependent transcription. However, RB family members also possess additional functions...
  9. Weng L, Zhu C, Xu J, Du W. Critical role of active repression by E2F and Rb proteins in endoreplication during Drosophila development. EMBO J. 2003;22:3865-75 pubmed
    ..Interestingly, the dE2F1(su89) mutant, which has E2F activation by dE2F1(su89) and active repression by dE2F2, is viable and fertile with no gross developmental defects...

More Information


  1. Buttitta L, Katzaroff A, Perez C, de la Cruz A, Edgar B. A double-assurance mechanism controls cell cycle exit upon terminal differentiation in Drosophila. Dev Cell. 2007;12:631-43 pubmed
    ..Enforcing both E2F and Cyclin/Cdk activities is required to bypass exit because feedback between E2F and Cyclin E/Cdk2 is inhibited ..
  2. Stevaux O, Dimova D, Ji J, Moon N, Frolov M, Dyson N. Retinoblastoma family 2 is required in vivo for the tissue-specific repression of dE2F2 target genes. Cell Cycle. 2005;4:1272-80 pubmed
    ..second Drosophila Retinoblastoma family gene (Rbf2), and contrasted the in vivo molecular functions of RBF2 with dE2F2, the only E2F partner of RBF2. Previous studies failed to uncover a unique role for RBF2 in E2F regulation...
  3. Hériché J, Ang D, Bier E, O Farrell P. Involvement of an SCFSlmb complex in timely elimination of E2F upon initiation of DNA replication in Drosophila. BMC Genet. 2003;4:9 pubmed
    ..A screen for mutations that dominantly modify this phenotype showed effective suppression upon reduction of E2F function, suggesting that compromised downregulation of E2F contributes to the phenotype...
  4. Frolov M, Huen D, Stevaux O, Dimova D, Balczarek Strang K, Elsdon M, et al. Functional antagonism between E2F family members. Genes Dev. 2001;15:2146-60 pubmed
    ..cells, that have a large number of E2F-related genes, the Drosophila genome encodes just two E2F genes, de2f1 and de2f2. Here we show that de2f1 and de2f2 provide different elements of E2F regulation and that they have opposing ..
  5. Harbour J, Dean D. The Rb/E2F pathway: expanding roles and emerging paradigms. Genes Dev. 2000;14:2393-409 pubmed
  6. Nicolay B, Frolov M. Context-dependent requirement for dE2F during oncogenic proliferation. PLoS Genet. 2008;4:e1000205 pubmed publisher
    ..In Drosophila, there is a single activator, dE2F1, and a single repressor, dE2F2, which act antagonistically to each other during development...
  7. Wichmann A, Uyetake L, Su T. E2F1 and E2F2 have opposite effects on radiation-induced p53-independent apoptosis in Drosophila. Dev Biol. 2010;346:80-9 pubmed publisher
    ..In contrast, the second E2F homolog, dE2F2, as well as the net E2F activity, which can be depleted by mutating the common cofactor, dDp, is inhibitory for ..
  8. Bangi E, Garza D, Hild M. In vivo analysis of compound activity and mechanism of action using epistasis in Drosophila. J Chem Biol. 2011;4:55-68 pubmed publisher
    ..The online version of this article (doi:10.1007/s12154-010-0051-5) contains supplementary material, which is available to authorized users. ..
  9. Lu J, Ruhf M, Perrimon N, Leder P. A genome-wide RNA interference screen identifies putative chromatin regulators essential for E2F repression. Proc Natl Acad Sci U S A. 2007;104:9381-6 pubmed
    ..studies have suggested that the Rb tumor suppressor may recruit multiple chromatin regulatory proteins to repress E2F, a key regulator of cell proliferation and differentiation...
  10. Lewis P, Sahoo D, Geng C, Bell M, Lipsick J, Botchan M. Drosophila lin-52 acts in opposition to repressive components of the Myb-MuvB/dREAM complex. Mol Cell Biol. 2012;32:3218-27 pubmed publisher
    ..Furthermore, these findings support a model in which the Lin-52 and Myb proteins counteract the repressive activities of the other members of the MMB/dREAM complex at specific genomic loci in a developmentally controlled manner. ..
  11. Beall E, Lewis P, Bell M, Rocha M, Jones D, Botchan M. Discovery of tMAC: a Drosophila testis-specific meiotic arrest complex paralogous to Myb-Muv B. Genes Dev. 2007;21:904-19 pubmed
    ..Together, these data demonstrate that MMB serves diverse roles in different developmental pathways, and members of MMB can be found in alternative, noninteracting complexes in different cell types. ..
  12. Parakati R, DiMario J. Repression of fibroblast growth factor receptor 1 gene expression by E2F4 in skeletal muscle cells. Dev Dyn. 2005;232:119-30 pubmed
    ..Immunodetection studies revealed that endogenous E2F1 and E2F2 proteins were cytoplasmic in myoblasts and myotubes, whereas E2F4 was abundant in the nuclei of both...
  13. Moon N, Frolov M, Kwon E, Di Stefano L, Dimova D, Morris E, et al. Drosophila E2F1 has context-specific pro- and antiapoptotic properties during development. Dev Cell. 2005;9:463-75 pubmed
    b>E2F transcription factors are generally believed to be positive regulators of apoptosis. In this study, we show that dE2F1 and dDP are important for the normal pattern of DNA damage-induced apoptosis in Drosophila wing discs...
  14. Blanchard D, Georlette D, Antoszewski L, Botchan M. Chromatin reader L(3)mbt requires the Myb-MuvB/DREAM transcriptional regulatory complex for chromosomal recruitment. Proc Natl Acad Sci U S A. 2014;111:E4234-43 pubmed publisher
    ..Mip120 (Lin54) is required for L(3)mbt recruitment to chromosomes, whereas Mip130 (Lin9) (an MMB core protein) and E2f2 (an MMB transcriptional repressor) are not, but are essential for repression...
  15. Muzzopappa M, Wappner P. Multiple roles of the F-box protein Slimb in Drosophila egg chamber development. Development. 2005;132:2561-71 pubmed
    ..By analyzing slmb germline clones, we found that loss of Slmb provoked a reduction in E2f2 and Dp levels, which correlated with misregulation of mitotic cycles during cyst formation, abnormal nurse cell ..
  16. Buttitta L, Katzaroff A, Edgar B. A robust cell cycle control mechanism limits E2F-induced proliferation of terminally differentiated cells in vivo. J Cell Biol. 2010;189:981-96 pubmed publisher
    ..cells in Drosophila melanogaster wings and eyes are largely resistant to proliferation upon deregulation of either E2F or cyclin E (CycE), but exogenous expression of both factors together can bypass cell cycle exit...
  17. Acharya P, Raj N, Buckley M, Zhang L, Duperon S, Williams G, et al. Paradoxical instability-activity relationship defines a novel regulatory pathway for retinoblastoma proteins. Mol Biol Cell. 2010;21:3890-901 pubmed publisher
    ..repressor potential is down-regulated by Cdk-dependent phosphorylation, resulting in repressor dissociation from E2F family transcription factors. Second, RB proteins are subject to proteasome-mediated destruction during development...
  18. Attwooll C, Lazzerini Denchi E, Helin K. The E2F family: specific functions and overlapping interests. EMBO J. 2004;23:4709-16 pubmed
    The E2F transcription factors are key regulators of cell cycle progression and the E2F field has made rapid advances since its advent in 1986...
  19. Wei Y, Mondal S, Mouawad R, Wilczyński B, Henry R, Arnosti D. Genome-Wide Analysis of Drosophila RBf2 Protein Highlights the Diversity of RB Family Targets and Possible Role in Regulation of Ribosome Biosynthesis. G3 (Bethesda). 2015;5:1503-15 pubmed publisher
    ..A previous model for RB-E2F interactions suggested that RBf1 binds dE2F1 or dE2F2, whereas RBf2 is restricted to binding to dE2F2; however, we found that RBf2 targets approximately twice as many ..
  20. Bohla D, Herold M, Panzer I, Buxa M, Ali T, Demmers J, et al. A functional insulator screen identifies NURF and dREAM components to be required for enhancer-blocking. PLoS ONE. 2014;9:e107765 pubmed publisher
    ..Furthermore, the enhancer blocking function of insulators is associated with nucleosomal depletion and requires NURF and dREAM. ..
  21. Singer R, Atar S, Atias O, Oron E, Segal D, Hirsch J, et al. Drosophila COP9 signalosome subunit 7 interacts with multiple genomic loci to regulate development. Nucleic Acids Res. 2014;42:9761-70 pubmed publisher
  22. Baumgardt M, Karlsson D, Salmani B, Bivik C, MacDonald R, Gunnar E, et al. Global programmed switch in neural daughter cell proliferation mode triggered by a temporal gene cascade. Dev Cell. 2014;30:192-208 pubmed publisher
    ..act combinatorially to control the precise timing of neuroblast cell-cycle exit by repressing Cyclin E and E2f. This reveals a logical principle underlying progenitor and daughter cell proliferation control in the Drosophila ..
  23. Ghavi Helm Y, Klein F, Pakozdi T, Ciglar L, Noordermeer D, Huber W, et al. Enhancer loops appear stable during development and are associated with paused polymerase. Nature. 2014;512:96-100 pubmed publisher
    ..Our results indicate that the general topology governing enhancer contacts is conserved from flies to humans and suggest that transcription initiates from preformed enhancer-promoter loops through release of paused polymerase. ..
  24. Zappia M, Frolov M. E2F function in muscle growth is necessary and sufficient for viability in Drosophila. Nat Commun. 2016;7:10509 pubmed publisher
    ..Interestingly, E2f1 provides a major contribution to the regulation of myogenic function, while E2f2 appears to be less important...
  25. Miles W, Tschop K, Herr A, Ji J, Dyson N. Pumilio facilitates miRNA regulation of the E2F3 oncogene. Genes Dev. 2012;26:356-68 pubmed publisher
    b>E2F transcription factors are important regulators of cell proliferation and are frequently dysregulated in human malignancies...
  26. Rasheva V, Knight D, Bozko P, Marsh K, Frolov M. Specific role of the SR protein splicing factor B52 in cell cycle control in Drosophila. Mol Cell Biol. 2006;26:3468-77 pubmed
    ..In Drosophila there are two E2F genes, an activator, de2f1, and a repressor, de2f2. The loss of de2f1 gives rise to the G(1)/S block accompanied by the repression of E2F-dependent transcription...
  27. Clavier A, Baillet A, Rincheval Arnold A, Coléno Costes A, Lasbleiz C, Mignotte B, et al. The pro-apoptotic activity of Drosophila Rbf1 involves dE2F2-dependent downregulation of diap1 and buffy mRNA. Cell Death Dis. 2014;5:e1405 pubmed publisher
    ..We found that Rbf1-induced apoptosis depends on dE2F2/dDP heterodimer, whereas dE2F1 transcriptional activity is not required...
  28. Rimkus S, Katzenberger R, Trinh A, Dodson G, Tibbetts R, Wassarman D. Mutations in String/CDC25 inhibit cell cycle re-entry and neurodegeneration in a Drosophila model of Ataxia telangiectasia. Genes Dev. 2008;22:1205-20 pubmed publisher
    ..Thus, we hypothesize that ATM performs a cell cycle checkpoint function to protect post-mitotic neurons from degeneration and that cell cycle re-entry causes neurodegeneration in A-T...
  29. Tanaka Matakatsu M, Xu J, Cheng L, Du W. Regulation of apoptosis of rbf mutant cells during Drosophila development. Dev Biol. 2009;326:347-56 pubmed publisher
    ..cues as well as to radiation and demonstrated that this hid enhancer is directly repressed by RBF through an E2F binding site. These observations indicate that apoptosis of rbf mutant cells is mediated by an upregulation of hid...
  30. Zielke N, Kim K, Tran V, Shibutani S, Bravo M, Nagarajan S, et al. Control of Drosophila endocycles by E2F and CRL4(CDT2). Nature. 2011;480:123-7 pubmed publisher
    ..Many of the regulatory interactions essential to this novel cell cycle oscillator are conserved in animals and plants, indicating that elements of this mechanism act in most growth-dependent cell cycles. ..
  31. Bhaskar P, Surabhi S, Tripathi B, Mukherjee A, Mutsuddi M. dLin52 is crucial for dE2F and dRBF mediated transcriptional regulation of pro-apoptotic gene hid. Biochim Biophys Acta. 2014;1839:800-12 pubmed publisher
    ..b>E2F and its corepressor RBF also belong to this complex and together they regulate expression of genes involved in cell ..
  32. Lee H, Ragusano L, Martinez A, Gill J, Dimova D. A dual role for the dREAM/MMB complex in the regulation of differentiation-specific E2F/RB target genes. Mol Cell Biol. 2012;32:2110-20 pubmed publisher
    ..First, it is required for the stability of the repressive dE2F2/RBF complexes at their promoters during S phase...
  33. Zraly C, Marenda D, Dingwall A. SNR1 (INI1/SNF5) mediates important cell growth functions of the Drosophila Brahma (SWI/SNF) chromatin remodeling complex. Genetics. 2004;168:199-214 pubmed
    ..Thus, in addition to important functions of the Brm complex in G1-S control, the complex also appears to be important for transcription of genes required for cell cycle progression. ..
  34. Cayirlioglu P, Bonnette P, Dickson M, Duronio R. Drosophila E2f2 promotes the conversion from genomic DNA replication to gene amplification in ovarian follicle cells. Development. 2001;128:5085-98 pubmed
    Drosophila contains two members of the E2F transcription factor family (E2f and E2f2), which controls the expression of genes that regulate the G1-S transition of the cell cycle...
  35. Lim Y, Yamasaki Y, Tsuda L. Ebi alleviates excessive growth signaling through multiple epigenetic functions in Drosophila. Genes Cells. 2013;18:909-20 pubmed publisher
    ..RBF), a Drosophila homologue of retinoblastoma, and regulating the expression of specific target genes of the Rbf/E2F pathway...
  36. Nicolay B, Bayarmagnai B, Islam A, Lopez Bigas N, Frolov M. Cooperation between dE2F1 and Yki/Sd defines a distinct transcriptional program necessary to bypass cell cycle exit. Genes Dev. 2011;25:323-35 pubmed publisher
    ..We suggest that such functional interactions would influence Yki activity and help diversify the transcriptional output of the Hippo pathway. ..
  37. Miles W, Korenjak M, Griffiths L, Dyer M, Provero P, Dyson N. Post-transcriptional gene expression control by NANOS is up-regulated and functionally important in pRb-deficient cells. EMBO J. 2014;33:2201-15 pubmed publisher
    ..we have looked for sets of evolutionary conserved, functionally related genes that are direct targets of pRb/E2F proteins...
  38. Debruhl H, Wen H, Lipsick J. The complex containing Drosophila Myb and RB/E2F2 regulates cytokinesis in a histone H2Av-dependent manner. Mol Cell Biol. 2013;33:1809-18 pubmed publisher
    ..Myb functions as an activator in the generally repressive Drosophila RBF, E2F2, and Myb (dREAM)/Myb-MuvB complex...
  39. Gómez Lamarca M, Cobreros Reguera L, Ibáñez Jiménez B, Palacios I, Martín Bermudo M. Integrins regulate epithelial cell differentiation by modulating Notch activity. J Cell Sci. 2014;127:4667-78 pubmed publisher
    ..Taken together, our findings reveal a direct involvement of integrin signaling on the spatial and temporal regulation of epithelial cell differentiation during development. ..
  40. Korenjak M, Kwon E, Morris R, Anderssen E, Amzallag A, Ramaswamy S, et al. dREAM co-operates with insulator-binding proteins and regulates expression at divergently paired genes. Nucleic Acids Res. 2014;42:8939-53 pubmed publisher
    ..Here we report a striking and unexpected overlap between dE2F2/dREAM sites and binding sites for the insulator-binding proteins CP190 and Beaf-32...
  41. Rovani M, Brachmann C, Ramsay G, Katzen A. The dREAM/Myb-MuvB complex and Grim are key regulators of the programmed death of neural precursor cells at the Drosophila posterior wing margin. Dev Biol. 2012;372:88-102 pubmed publisher
    ..multi-subunit complex, which in addition to four Myb-interacting proteins including Mip130, contains repressive E2F and pRB proteins...
  42. Korenjak M, Brehm A. E2F-Rb complexes regulating transcription of genes important for differentiation and development. Curr Opin Genet Dev. 2005;15:520-7 pubmed