Gene Symbol: DNApol-alpha73
Description: DNA polymerase alpha 73kD
Alias: CG5923, DNA pol-73, DNA polalpha, DNA polymerase alpha 73 kD, DNApol-alpha, DNApol-alpha72, DNApolalpha, Dmel\CG5923, Dmpol alpha, POLA, Pol, Polalpha, Polalpha 73kDa, pol alpha, polalpha, DNA polymerase alpha 73kD, B subunit, CG5923-PC, DNA polymerase 73K, DNA polymerase alpha, DNA polymerase alpha primase 73 kda subunit, DNA-polymerase alpha, DNApol-alpha73-PC
Species: fruit fly

Top Publications

  1. Royzman I, Orr Weaver T. S phase and differential DNA replication during Drosophila oogenesis. Genes Cells. 1998;3:767-76 pubmed
    ..Cyclin E and E2F are needed for this differential DNA replication during Drosophila oogenesis. ..
  2. Shen J, Creighton S, Jones P, Goodman M. A comparison of the fidelity of copying 5-methylcytosine and cytosine at a defined DNA template site. Nucleic Acids Res. 1992;20:5119-25 pubmed
    ..mismatched base pairs at the mC position using three DNA polymerases, AMV reverse transcriptase, Drosophila DNA polymerase alpha, and mutant Escherichia coli Klenow fragment containing no proofreading exonuclease activity...
  3. Frolov M, Stevaux O, Moon N, Dimova D, Kwon E, Morris E, et al. G1 cyclin-dependent kinases are insufficient to reverse dE2F2-mediated repression. Genes Dev. 2003;17:723-8 pubmed
    ..The implication of these results is that cells containing dE2F2 require dE2F1 to either prevent, or reverse, dE2F-mediated repression. ..
  4. Fuss B, Meissner T, Bauer R, Lehmann C, Eckardt F, Hoch M. Control of endoreduplication domains in the Drosophila gut by the knirps and knirps-related genes. Mech Dev. 2001;100:15-23 pubmed
    ..Our results provide a novel link between morphogen-dependent positional information and the spatio-temporal regulation of cell cycle activity in the gut. ..
  5. Kamakaka R, Kaufman P, Stillman B, Mitsis P, Kadonaga J. Simian virus 40 origin- and T-antigen-dependent DNA replication with Drosophila factors in vitro. Mol Cell Biol. 1994;14:5114-22 pubmed
    ..Moreover, the observation that SV40 DNA replication can be performed with Drosophila factors provides a useful assay for the study of bidirectional DNA replication in Drosophila species in the context of a complete replication reaction. ..
  6. Marton R, Thommes P, Cotterill S. Purification and characterisation of dRP-A: a single-stranded DNA binding protein from Drosophila melanogaster. FEBS Lett. 1994;342:139-44 pubmed
    ..We also demonstrate that dRP-A can stimulate the activity of its homologous DNA polymerase alpha in excess of 20 fold...
  7. Sasaki T, Sawado T, Yamaguchi M, Shinomiya T. Specification of regions of DNA replication initiation during embryogenesis in the 65-kilobase DNApolalpha-dE2F locus of Drosophila melanogaster. Mol Cell Biol. 1999;19:547-55 pubmed
    ..In the DNA polymerase alpha gene (DNApolalpha) locus, where an initiation region, oriDalpha, had been identified in cultured Kc cells, ..
  8. Leclerc V, Tassan J, O Farrell P, Nigg E, Leopold P. Drosophila Cdk8, a kinase partner of cyclin C that interacts with the large subunit of RNA polymerase II. Mol Biol Cell. 1996;7:505-13 pubmed
    ..Based on these observations and sequence similarity to the kinase/cyclin pair Srb10/Srb11 in S. cerevisiae, we suggest that cyclin C and Cdk8 control RNA polymerase II function. ..
  9. Cotterill S, Lehman I, McLachlan P. Cloning of the gene for the 73 kD subunit of the DNA polymerase alpha primase of Drosophila melanogaster. Nucleic Acids Res. 1992;20:4325-30 pubmed
    We have isolated both cDNA and genomic clones for the 73 kDa subunit of the DNA polymerase alpha primase of Drosophila melanogaster. Analysis of these clones has identified an open reading frame of 1959 bases coding for a protein of 72...

More Information


  1. Kuroda K, Ueda R. A 130 kDa polypeptide immunologically related to the 180 kDa catalytic subunit of DNA polymerase alpha-primase complex is detected in early embryos of Drosophila. J Biochem. 1995;117:809-18 pubmed
    An immunocytochemical method using specific antibodies was employed to detect DNA polymerase alpha-primase complex in Drosophila melanogaster embryos during the first 13 nuclear division cycles...
  2. Mitsis P, Kowalczykowski S, Lehman I. A single-stranded DNA binding protein from Drosophila melanogaster: characterization of the heterotrimeric protein and its interaction with single-stranded DNA. Biochemistry. 1993;32:5257-66 pubmed
    ..D-SSB stimulates the extent and processivity of DNA synthesis of its cognate DNA polymerase alpha. On the basis of these properties, we conclude that D-SSB is the Drosophila cognate of the human and yeast ..
  3. Suyari O, Kawai M, Ida H, Yoshida H, Sakaguchi K, Yamaguchi M. Differential requirement for the N-terminal catalytic domain of the DNA polymerase ? p255 subunit in the mitotic cell cycle and the endocycle. Gene. 2012;495:104-14 pubmed publisher
    ..dpol?p255 carries well-conserved six DNA polymerase subdomains and five 3'?5' exonuclease motifs as observed with Pol? in other species...
  4. Takahashi Y, Yamaguchi M, Hirose F, Cotterill S, Kobayashi J, Miyajima S, et al. DNA replication-related elements cooperate to enhance promoter activity of the drosophila DNA polymerase alpha 73-kDa subunit gene. J Biol Chem. 1996;271:14541-7 pubmed
    An analysis was carried out on the promoter region of the Drosophila DNA polymerase alpha 73-kDa subunit gene and the factor(s) activating the promoter...
  5. Smith A, King J, Orr Weaver T. Identification of genomic regions required for DNA replication during Drosophila embryogenesis. Genetics. 1993;135:817-29 pubmed
    ..Additionally, our analysis identified a gene, pimples, that is required for the proper completion of mitosis in the post-blastoderm divisions of the embryo. ..
  6. Mitsis P, Chiang C, Lehman I. Purification of DNA polymerase-primase (DNA polymerase alpha) and DNA polymerase delta from embryos of Drosophila melanogaster. Methods Enzymol. 1995;262:62-77 pubmed
  7. Yamaguchi M, Hirose F, Nishimoto Y, Naruge T, Ikeda M, Hachiya T, et al. Expression patterns of DNA replication enzymes and the regulatory factor DREF during Drosophila development analyzed with specific antibodies. Biol Cell. 1995;85:147-55 pubmed
    ..Using these antibodies together with those for DNA polymerase alpha and proliferating cell nuclear antigen (PCNA), we examined expression patterns and sub-cellular ..
  8. Kuroda K, Ueda R. Phosphorylation and dephosphorylation of the B subunit of DNA polymerase alpha-primase complex in the early embryogenesis of Drosophila. Biochem Biophys Res Commun. 1999;254:372-7 pubmed
    In the early embryos of Drosophila, the B subunit of the DNA polymerase alpha-primase complex was found to migrate more slowly during the first 13 mitotic cycles than that from cycle 14 using SDS-polyacrylamide gel electrophoresis...
  9. Cai H, Bloom L, Eritja R, Goodman M. Kinetics of deoxyribonucleotide insertion and extension at abasic template lesions in different sequence contexts using HIV-1 reverse transcriptase. J Biol Chem. 1993;268:23567-72 pubmed
    ..A striking result was that while primers were extended past an abasic lesion by HIV-1 RT in both direct and misalignment modes, avian myeloblastosis virus RT failed to catalyze significant extension by either mode...
  10. Kaguni L, Rossignol J, Conaway R, Lehman I. Isolation of an intact DNA polymerase-primase from embryos of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1983;80:2221-5 pubmed
    A procedure has been devised for the purification of intact DNA polymerase alpha from early embryos of Drosophila melanogaster. The purified enzyme consists of at least three polypeptides with Mrs of 182,000, 60,000, and 50,000...
  11. Crevel G, Bates H, Huikeshoven H, Cotterill S. The Drosophila Dpit47 protein is a nuclear Hsp90 co-chaperone that interacts with DNA polymerase alpha. J Cell Sci. 2001;114:2015-25 pubmed
    ..protein (DNA polymerase interacting tpr containing protein of 47 kDa) through its interaction with the DNA polymerase alpha. This protein is a predominantly nuclear protein, which forms a tight and stoichiometric interaction with ..
  12. Singer B, Dosanjh M. Site-directed mutagenesis for quantitation of base-base interactions at defined sites. Mutat Res. 1990;233:45-51 pubmed
    ..All enzymes led to approximately the same frequency of transitions. It is postulated that the mutation frequency at a given site is primarily a function of the structure of the sequence around the target site. ..
  13. Loppin B, Berger F, Couble P. The Drosophila maternal gene s├ęsame is required for sperm chromatin remodeling at fertilization. Chromosoma. 2001;110:430-40 pubmed
    ..The abnormal chromatin organization of the male pronucleus does not prevent the formation of a male pronuclear envelope, which breaks down and reassembles in synchrony with maternally derived nuclei present in the same cytoplasm. ..
  14. Mizushina Y, Murakami C, Yogi K, Ueda K, Ishidoh T, Takemura M, et al. Kohamaic acid A, a novel sesterterpenic acid, inhibits activities of DNA polymerases from deuterostomes. Biochim Biophys Acta. 2003;1648:55-61 pubmed
    ..In this paper, we report that this compound could selectively inhibit the activities of DNA polymerases (pol. alpha, beta, gamma, delta and epsilon ) only from species in the deuterostome branch in the animal kingdom, like ..
  15. Dosanjh M, Essigmann J, Goodman M, Singer B. Comparative efficiency of forming m4T.G versus m4T.A base pairs at a unique site by use of Escherichia coli DNA polymerase I (Klenow fragment) and Drosophila melanogaster polymerase alpha-primase complex. Biochemistry. 1990;29:4698-703 pubmed
    ..coli DNA polymerase I (Klenow fragment, Kf) and Drosophila melanogaster polymerase alpha-primase complex (pol alpha) were used. On the basis of the Vmax/Km ratios, the pairing of m4T.G was preferred over that of both m4T.A and T...
  16. Takeuchi R, Oshige M, Uchida M, Ishikawa G, Takata K, Shimanouchi K, et al. Purification of Drosophila DNA polymerase zeta by REV1 protein-affinity chromatography. Biochem J. 2004;382:535-43 pubmed
    ..Our aim was to determine and elucidate the biochemical properties of one such polymerase, pol zeta (DNA polymerase zeta) from Drosophila melanogaster (Dmpol zeta)...