Gene Symbol: DNApol-alpha180
Description: DNA polymerase alpha 180kD
Alias: 183 kDa pol alpha, AAB24152, CG6349, DNA Pol-alpha180, DNA polalpha, DNA polymerase alpha 180 kD, DNA-Polalpha, DNApol, DNApol-alpha, DNApola, DNApolalpha, DNApolalpha180, DmPolalpha, Dmel\CG6349, Dmpol alpha, E(mus304), POLA, POlalpha 180kDa, Pol, Pol alpha, Polalpha, dpolalphap180, pol alpha, polalpha, polalphap180, DNA polymerase alpha 180kD, 180 kDa subunit, 180 kDa subunit of polymerase-primase, CG6349-PA, DNA pol 180, DNA polymerase 180K, DNA polymerase alpha, DNA polymerase alpha 148,000 alpha subunit, DNA polymerase alpha 180 kD, DNA polymerase alpha 180-kDa subunit, DNA polymerase alpha 182,000 alpha subunit, DNA polymerase-primase 180 kda subunit, DNA-polymerase alpha, DNApol-alpha180-PA, enhancer of mus304
Species: fruit fly

Top Publications

  1. Shen J, Creighton S, Jones P, Goodman M. A comparison of the fidelity of copying 5-methylcytosine and cytosine at a defined DNA template site. Nucleic Acids Res. 1992;20:5119-25 pubmed
    ..mismatched base pairs at the mC position using three DNA polymerases, AMV reverse transcriptase, Drosophila DNA polymerase alpha, and mutant Escherichia coli Klenow fragment containing no proofreading exonuclease activity...
  2. Melov S, Vaughan H, Cotterill S. Molecular characterisation of the gene for the 180 kDa subunit of the DNA polymerase-primase of Drosophila melanogaster. J Cell Sci. 1992;102 ( Pt 4):847-56 pubmed
    We have cloned and sequenced the gene for the 180 kDa subunit of the a polymerase from Drosophila melanogaster. The protein shows high similarity to the 180 kDa subunits from other species...
  3. Villani G, Fay P, Bambara R, Lehman I. Elongation of RNA-primed DNA templates by DNA polymerase alpha from Drosophila melanogaster embryos. J Biol Chem. 1981;256:8202-7 pubmed
  4. Hirose F, Yamaguchi M, Handa H, Inomata Y, Matsukage A. Novel 8-base pair sequence (Drosophila DNA replication-related element) and specific binding factor involved in the expression of Drosophila genes for DNA polymerase alpha and proliferating cell nuclear antigen. J Biol Chem. 1993;268:2092-9 pubmed
    ..DNA replication-related element (DRE)), are required for the high expression of Drosophila genes for DNA polymerase alpha and the proliferating cell nuclear antigen (PCNA) (an auxiliary protein for DNA polymerase delta)...
  5. Kamakaka R, Kaufman P, Stillman B, Mitsis P, Kadonaga J. Simian virus 40 origin- and T-antigen-dependent DNA replication with Drosophila factors in vitro. Mol Cell Biol. 1994;14:5114-22 pubmed
    ..Moreover, the observation that SV40 DNA replication can be performed with Drosophila factors provides a useful assay for the study of bidirectional DNA replication in Drosophila species in the context of a complete replication reaction. ..
  6. Kuroda K, Kagiyama Takahashi R, Okuda A, Omori A. Identification of the specific labile sites in the 180 kDa catalytic polypeptide of the Drosophila melanogaster DNA polymerase alpha-primase complex. J Biochem. 1993;113:126-8 pubmed
    The immunoaffinity-purified DNA polymerase alpha-primase complex from Drosophila melanogaster Kc cells contains three high molecular weight polypeptides besides the 180 kDa catalytic polypeptide...
  7. Sahashi R, Crevel G, Pasko J, Suyari O, Nagai R, Saura M, et al. DNA polymerase ? interacts with PrSet7 and mediates H4K20 monomethylation in Drosophila. J Cell Sci. 2014;127:3066-78 pubmed publisher
  8. LaFave M, Andersen S, Stoffregen E, Holsclaw J, Kohl K, Overton L, et al. Sources and structures of mitotic crossovers that arise when BLM helicase is absent in Drosophila. Genetics. 2014;196:107-18 pubmed publisher
    ..These findings provide important new insights into sources and structures of mitotic crossovers and functions of BLM helicase. ..
  9. Delabaere L, Orsi G, Sapey Triomphe L, Horard B, Couble P, Loppin B. The Spartan ortholog maternal haploid is required for paternal chromosome integrity in the Drosophila zygote. Curr Biol. 2014;24:2281-7 pubmed publisher

More Information


  1. Dosanjh M, Essigmann J, Goodman M, Singer B. Comparative efficiency of forming m4T.G versus m4T.A base pairs at a unique site by use of Escherichia coli DNA polymerase I (Klenow fragment) and Drosophila melanogaster polymerase alpha-primase complex. Biochemistry. 1990;29:4698-703 pubmed
    ..coli DNA polymerase I (Klenow fragment, Kf) and Drosophila melanogaster polymerase alpha-primase complex (pol alpha) were used. On the basis of the Vmax/Km ratios, the pairing of m4T.G was preferred over that of both m4T.A and T...
  2. Royzman I, Orr Weaver T. S phase and differential DNA replication during Drosophila oogenesis. Genes Cells. 1998;3:767-76 pubmed
    ..Cyclin E and E2F are needed for this differential DNA replication during Drosophila oogenesis. ..
  3. Bosco G, Du W, Orr Weaver T. DNA replication control through interaction of E2F-RB and the origin recognition complex. Nat Cell Biol. 2001;3:289-95 pubmed
    ..Our results indicate that dE2F1 and Rbf function together at replication origins to limit DNA replication through interactions with DmORC. ..
  4. Frolov M, Stevaux O, Moon N, Dimova D, Kwon E, Morris E, et al. G1 cyclin-dependent kinases are insufficient to reverse dE2F2-mediated repression. Genes Dev. 2003;17:723-8 pubmed
    ..The implication of these results is that cells containing dE2F2 require dE2F1 to either prevent, or reverse, dE2F-mediated repression. ..
  5. Donaghue C, Bates H, Cotterill S. Identification and characterisation of the Drosophila homologue of the yeast Uba2 gene. Biochim Biophys Acta. 2001;1518:210-4 pubmed
    ..The nature of these sites is not clear, however the failure of dUba2 to localise to the sites of chorion amplification in ovaries suggests that they are not sites of ongoing DNA replication. ..
  6. Hyun J, Jasper H, Bohmann D. DREF is required for efficient growth and cell cycle progression in Drosophila imaginal discs. Mol Cell Biol. 2005;25:5590-8 pubmed
    ..These data establish DREF as a global regulator of transcriptional programs that mediate cell proliferation and organ growth during animal development. ..
  7. Lee H, Ragusano L, Martinez A, Gill J, Dimova D. A dual role for the dREAM/MMB complex in the regulation of differentiation-specific E2F/RB target genes. Mol Cell Biol. 2012;32:2110-20 pubmed publisher
    ..First, it is required for the stability of the repressive dE2F2/RBF complexes at their promoters during S phase. Second, we find that dREAM is indispensable for both transcriptional repression mechanisms employed at these genes. ..
  8. Davidson I. The genetics of TBP and TBP-related factors. Trends Biochem Sci. 2003;28:391-8 pubmed
  9. Villani G, Sauer B, Lehman I. DNA polymerase alpha from Drosophila melanogaster embryos. Subunit structure. J Biol Chem. 1980;255:9479-83 pubmed
    The homogeneous DNA polymerase alpha from early embryos of Drosophila melanogaster contains four polypeptides designated alpha, beta, gamma, and delta, with molecular weights of 148,000, 58,000, 46,000, and 43,000, respectively (Banks, G...
  10. Yamaguchi M, Hayashi Y, Hirose F, Nishimoto Y, Matsukage A. Distinct roles of E2F recognition sites as positive or negative elements in regulation of the DNA polymerase alpha 180 kDa catalytic subunit gene promoter during Drosophila development. Nucleic Acids Res. 1997;25:3847-54 pubmed
    ..GST)-E2F and GST-DP fusion proteins were found to cooperate in binding to the three E2F sites in the DNA polymerase alpha gene promoter in vitro...
  11. Banks G, Boezi J, Lehman I. A high molecular weight DNA polymerase from Drosophila melanogaster embryos. Purification, structure, and partial characterization. J Biol Chem. 1979;254:9886-92 pubmed
    ..It was inhibited by low concentrations of N-ethylmaleimide and Aphidicolon. Thus the DNA polymerase of D. melanogaster resembles most closely the alpha-DNA polymerases that have been purified from mammalian cells. ..
  12. Mitsis P, Chiang C, Lehman I. Purification of DNA polymerase-primase (DNA polymerase alpha) and DNA polymerase delta from embryos of Drosophila melanogaster. Methods Enzymol. 1995;262:62-77 pubmed
  13. Crevel G, Bates H, Huikeshoven H, Cotterill S. The Drosophila Dpit47 protein is a nuclear Hsp90 co-chaperone that interacts with DNA polymerase alpha. J Cell Sci. 2001;114:2015-25 pubmed
    ..protein (DNA polymerase interacting tpr containing protein of 47 kDa) through its interaction with the DNA polymerase alpha. This protein is a predominantly nuclear protein, which forms a tight and stoichiometric interaction with ..
  14. Kaguni L, Rossignol J, Conaway R, Banks G, Lehman I. Association of DNA primase with the beta/gamma subunits of DNA polymerase alpha from Drosophila melanogaster embryos. J Biol Chem. 1983;258:9037-9 pubmed
    The DNA polymerase and primase activities of the intact DNA polymerase alpha from early embryos of Drosophila melanogaster co-sediment in native glycerol gradients. However, the activities are separated in glycerol gradients containing 2...
  15. Gosnell J, Christensen T. Drosophila Ctf4 is essential for efficient DNA replication and normal cell cycle progression. BMC Mol Biol. 2011;12:13 pubmed publisher
    ..We show that Ctf4 function is conserved and that Drosophila can be effectively used as a model to further probe the precise function of Ctf4 as a member of the replication fork and possible roles in development. ..
  16. Brodsky M, Sekelsky J, Tsang G, Hawley R, Rubin G. mus304 encodes a novel DNA damage checkpoint protein required during Drosophila development. Genes Dev. 2000;14:666-78 pubmed
    ..Similar mechanisms may account for the puzzling array of symptoms observed in humans with mutations in the ATM tumor suppressor gene. ..
  17. Larocque J, Dougherty D, Hussain S, Sekelsky J. Reducing DNA polymerase alpha in the absence of Drosophila ATR leads to P53-dependent apoptosis and developmental defects. Genetics. 2007;176:1441-51 pubmed
    ..We found that E(mus304) is a mutation in DNApol-alpha180, which encodes the catalytic subunit of DNA polymerase alpha. We did not find any defects resulting from reducing Polalpha by itself...
  18. Leclerc V, Tassan J, O Farrell P, Nigg E, Leopold P. Drosophila Cdk8, a kinase partner of cyclin C that interacts with the large subunit of RNA polymerase II. Mol Biol Cell. 1996;7:505-13 pubmed
    ..Based on these observations and sequence similarity to the kinase/cyclin pair Srb10/Srb11 in S. cerevisiae, we suggest that cyclin C and Cdk8 control RNA polymerase II function. ..
  19. Hirose F, Yamaguchi M, Kuroda K, Omori A, Hachiya T, Ikeda M, et al. Isolation and characterization of cDNA for DREF, a promoter-activating factor for Drosophila DNA replication-related genes. J Biol Chem. 1996;271:3930-7 pubmed
    ..The antibodies inhibited specifically the transcription of DNA polymerase alpha promoter in vitro...
  20. Singer B, Dosanjh M. Site-directed mutagenesis for quantitation of base-base interactions at defined sites. Mutat Res. 1990;233:45-51 pubmed
    ..All enzymes led to approximately the same frequency of transitions. It is postulated that the mutation frequency at a given site is primarily a function of the structure of the sequence around the target site. ..
  21. Kugler S, Nagel A. putzig is required for cell proliferation and regulates notch activity in Drosophila. Mol Biol Cell. 2007;18:3733-40 pubmed
    ..Our findings uncover different modes of operation of pzg during imaginal development of Drosophila, and they provide a novel mechanism of Notch regulation. ..
  22. Mizushina Y, Murakami C, Yogi K, Ueda K, Ishidoh T, Takemura M, et al. Kohamaic acid A, a novel sesterterpenic acid, inhibits activities of DNA polymerases from deuterostomes. Biochim Biophys Acta. 2003;1648:55-61 pubmed
    ..In this paper, we report that this compound could selectively inhibit the activities of DNA polymerases (pol. alpha, beta, gamma, delta and epsilon ) only from species in the deuterostome branch in the animal kingdom, like ..
  23. Burgers P, Koonin E, Bruford E, Blanco L, Burtis K, Christman M, et al. Eukaryotic DNA polymerases: proposal for a revised nomenclature. J Biol Chem. 2001;276:43487-90 pubmed
  24. Weiss S, Fisher P. Interaction of Drosophila DNA polymerase alpha holoenzyme with synthetic template-primers containing mismatched primer bases or propanodeoxyguanosine adducts at various positions in template and primer regions. J Biol Chem. 1992;267:18520-6 pubmed
    We studied recognition and binding of synthetic template-primers by Drosophila DNA polymerase alpha (pol alpha) holoenzyme...
  25. Ashburner M, Ball C, Blake J, Botstein D, Butler H, Cherry J, et al. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet. 2000;25:25-9 pubmed
  26. Kuroda K, Kagiyama Takahashi R, Shinomiya T. Immunoaffinity purification and properties of Drosophila melanogaster DNA polymerase alpha-primase complex. J Biochem. 1990;108:926-33 pubmed
    Two hybrid cell lines (DM88-5E12 and DM88-4C9) secreting monoclonal antibodies against DNA polymerase alpha-primase complex from Drosophila melanogaster Kc cells were established by immunizing mice with the complex partially purified by a ..
  27. Yamaguchi M, Hirose F, Nishimoto Y, Naruge T, Ikeda M, Hachiya T, et al. Expression patterns of DNA replication enzymes and the regulatory factor DREF during Drosophila development analyzed with specific antibodies. Biol Cell. 1995;85:147-55 pubmed
    ..Using these antibodies together with those for DNA polymerase alpha and proliferating cell nuclear antigen (PCNA), we examined expression patterns and sub-cellular ..
  28. Shinomiya T, Ina S. Mapping an initiation region of DNA replication at a single-copy chromosomal locus in Drosophila melanogaster cells by two-dimensional gel methods and PCR-mediated nascent-strand analysis: multiple replication origins in a broad zone. Mol Cell Biol. 1994;14:7394-403 pubmed
    ..The initiation region was first localized downstream of the DNA polymerase alpha gene by determining direction of replication forks with the neutral/alkaline 2D gel method...
  29. Hirose F, Yamaguchi M, Matsukage A. Targeted expression of the DNA binding domain of DRE-binding factor, a Drosophila transcription factor, attenuates DNA replication of the salivary gland and eye imaginal disc. Mol Cell Biol. 1999;19:6020-8 pubmed
    ..of the N-terminal fragment in salivary gland cells reduced the contents of mRNAs for the 180-kDa subunit of DNA polymerase alpha and for dE2F and the extent of DNA endoreplication...
  30. Goulian M, Heard C, Grimm S. Purification and properties of an accessory protein for DNA polymerase alpha/primase. J Biol Chem. 1990;265:13221-30 pubmed
    A protein that stimulates DNA polymerase alpha/primase many-fold on unprimed poly(dT) was purified to homogeneity from extracts of cultured mouse cells...
  31. Cai H, Bloom L, Eritja R, Goodman M. Kinetics of deoxyribonucleotide insertion and extension at abasic template lesions in different sequence contexts using HIV-1 reverse transcriptase. J Biol Chem. 1993;268:23567-72 pubmed
    ..A striking result was that while primers were extended past an abasic lesion by HIV-1 RT in both direct and misalignment modes, avian myeloblastosis virus RT failed to catalyze significant extension by either mode...
  32. Takeuchi R, Oshige M, Uchida M, Ishikawa G, Takata K, Shimanouchi K, et al. Purification of Drosophila DNA polymerase zeta by REV1 protein-affinity chromatography. Biochem J. 2004;382:535-43 pubmed
    ..Our aim was to determine and elucidate the biochemical properties of one such polymerase, pol zeta (DNA polymerase zeta) from Drosophila melanogaster (Dmpol zeta)...
  33. Sauer B, Lehman I. Immunological comparison of purified DNA polymerase alpha from embryos of Drosophila melanogaster with forms of the enzyme present in vivo. J Biol Chem. 1982;257:12394-8 pubmed
    Specific antisera to purified DNA polymerase alpha from embryos of Drosophila melanogaster and to two of the four constituent subunits (alpha, beta, gamma, and delta) were prepared...
  34. Marton R, Thommes P, Cotterill S. Purification and characterisation of dRP-A: a single-stranded DNA binding protein from Drosophila melanogaster. FEBS Lett. 1994;342:139-44 pubmed
    ..We also demonstrate that dRP-A can stimulate the activity of its homologous DNA polymerase alpha in excess of 20 fold...
  35. Hirose F, Yamaguchi M, Nishida Y, Masutani M, Miyazawa H, Hanaoka F, et al. Structure and expression during development of Drosophila melanogaster gene for DNA polymerase alpha. Nucleic Acids Res. 1991;19:4991-8 pubmed
    The Drosophila melanogaster gene and cDNA which span the entire open reading frame for DNA polymerase alpha, were cloned, and their nucleotide sequences were determined. The gene consists of 6 exons separated by 5 short introns...
  36. Suyari O, Kawai M, Ida H, Yoshida H, Sakaguchi K, Yamaguchi M. Differential requirement for the N-terminal catalytic domain of the DNA polymerase ? p255 subunit in the mitotic cell cycle and the endocycle. Gene. 2012;495:104-14 pubmed publisher
    ..dpol?p255 carries well-conserved six DNA polymerase subdomains and five 3'?5' exonuclease motifs as observed with Pol? in other species...
  37. Kuroda K, Ueda R. A 130 kDa polypeptide immunologically related to the 180 kDa catalytic subunit of DNA polymerase alpha-primase complex is detected in early embryos of Drosophila. J Biochem. 1995;117:809-18 pubmed
    An immunocytochemical method using specific antibodies was employed to detect DNA polymerase alpha-primase complex in Drosophila melanogaster embryos during the first 13 nuclear division cycles...
  38. Sasaki T, Sawado T, Yamaguchi M, Shinomiya T. Specification of regions of DNA replication initiation during embryogenesis in the 65-kilobase DNApolalpha-dE2F locus of Drosophila melanogaster. Mol Cell Biol. 1999;19:547-55 pubmed
    ..In the DNA polymerase alpha gene (DNApolalpha) locus, where an initiation region, oriDalpha, had been identified in cultured Kc cells, ..
  39. Hirose F, Yamaguchi M, Matsukage A. Repression of regulatory factor for Drosophila DNA replication-related gene promoters by zerknüllt homeodomain protein. J Biol Chem. 1994;269:2937-42 pubmed
    ..to +45 nucleotide positions with respect to the major transcription initiation site) of the Drosophila DNA polymerase alpha gene was repressed by cotransfection into Drosophila Kc cells with a zerknüllt (zen)-expressing plasmid as ..
  40. Peck V, Gerner E, Cress A. Delta-type DNA polymerase characterized from Drosophila melanogaster embryos. Nucleic Acids Res. 1992;20:5779-84 pubmed
    ..Two types of DNA polymerase, DNA polymerase delta and DNA polymerase alpha, were distinguished by: 1. copurification of DNA primase or 3'-5'exonuclease activities; 2...
  41. Kaguni L, Rossignol J, Conaway R, Lehman I. Isolation of an intact DNA polymerase-primase from embryos of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1983;80:2221-5 pubmed
    A procedure has been devised for the purification of intact DNA polymerase alpha from early embryos of Drosophila melanogaster. The purified enzyme consists of at least three polypeptides with Mrs of 182,000, 60,000, and 50,000...
  42. Mitsis P, Kowalczykowski S, Lehman I. A single-stranded DNA binding protein from Drosophila melanogaster: characterization of the heterotrimeric protein and its interaction with single-stranded DNA. Biochemistry. 1993;32:5257-66 pubmed
    ..D-SSB stimulates the extent and processivity of DNA synthesis of its cognate DNA polymerase alpha. On the basis of these properties, we conclude that D-SSB is the Drosophila cognate of the human and yeast ..
  43. Yamaguchi M, Kuroda K, Hirose F, Matsukage A. Distribution of DNA polymerase alpha during nuclear division cycles in Drosophila melanogaster embryo. Cell Struct Funct. 1992;17:105-12 pubmed
    An immunocytochemical method using a specific monoclonal antibody was employed to detect DNA polymerase alpha in Drosophila melanogaster embryos during the first 13 nuclear division cycles after fertilization...
  44. Mathe E, Bates H, Huikeshoven H, Deak P, Glover D, Cotterill S. Importin-alpha3 is required at multiple stages of Drosophila development and has a role in the completion of oogenesis. Dev Biol. 2000;223:307-22 pubmed
    The Drosophila importin-alpha3 gene was isolated through its interaction with the large subunit of the DNA polymerase alpha in a two-hybrid screen...
  45. Peck V, Gerner E, Cress A. A DNA polymerase alpha-associated 56 kDa protein kinase. Biochem Biophys Res Commun. 1993;190:325-31 pubmed
    ..We report that Drosophila embryo DNA polymerase alpha complex, isolated by immunological techniques, contains a protein kinase activity...