dia

Summary

Gene Symbol: dia
Description: diaphanous
Alias: 38E.16, CG1768, DIA, Dia, Dias, Dmel\CG1768, l(2)k07135, ms(2)04138, diaphanous, CG1768-PA, CG1768-PB, CG1768-PC, CG1768-PD, CG1768-PE, dia-PA, dia-PB, dia-PC, dia-PD, dia-PE
Species: fruit fly
Products:     dia

Top Publications

  1. Widmann T, Dahmann C. Dpp signaling promotes the cuboidal-to-columnar shape transition of Drosophila wing disc epithelia by regulating Rho1. J Cell Sci. 2009;122:1362-73 pubmed publisher
    ..Our results identify a cell-autonomous role for Dpp signaling in promoting and maintaining the elongated columnar shape of wing disc cells and suggest that Dpp signaling acts by regulating Rho1 and MRLC. ..
  2. Sansores Garcia L, Bossuyt W, Wada K, Yonemura S, Tao C, Sasaki H, et al. Modulating F-actin organization induces organ growth by affecting the Hippo pathway. EMBO J. 2011;30:2325-35 pubmed publisher
    ..formation, by loss of Capping proteins A or B, or caused by overexpression of an activated version of the formin Diaphanous, induced strong overgrowth in Drosophila imaginal discs through modulating the activity of the Hippo pathway...
  3. Grosshans J, Wenzl C, Herz H, Bartoszewski S, Schnorrer F, Vogt N, et al. RhoGEF2 and the formin Dia control the formation of the furrow canal by directed actin assembly during Drosophila cellularisation. Development. 2005;132:1009-20 pubmed
    ..We show that both the guanyl-nucleotide exchange factor RhoGEF2 and the formin Diaphanous (Dia) are required for furrow canal formation...
  4. Bilancia C, Winkelman J, Tsygankov D, Nowotarski S, Sees J, Comber K, et al. Enabled negatively regulates diaphanous-driven actin dynamics in vitro and in vivo. Dev Cell. 2014;28:394-408 pubmed publisher
    ..We characterized Diaphanous (Dia) and Enabled (Ena) as a model, using complementary approaches: cell culture, biophysical analysis, and ..
  5. Blanchard G, Murugesu S, Adams R, Martinez Arias A, Gorfinkiel N. Cytoskeletal dynamics and supracellular organisation of cell shape fluctuations during dorsal closure. Development. 2010;137:2743-52 pubmed publisher
    ..Our results suggest that cell shape fluctuations are a property of cells with low and increasing levels of apical myosin, and that medial and junctional myosin populations combine to contract AS cell apices and drive DC. ..
  6. Price M, Roberts D, McCartney B, Jezuit E, Peifer M. Cytoskeletal dynamics and cell signaling during planar polarity establishment in the Drosophila embryonic denticle. J Cell Sci. 2006;119:403-15 pubmed
    ..Finally, we define roles for the canonical Wingless and Hedgehog pathways and for core planar cell polarity proteins in denticle polarity. ..
  7. Warner S, Longmore G. Cdc42 antagonizes Rho1 activity at adherens junctions to limit epithelial cell apical tension. J Cell Biol. 2009;187:119-33 pubmed publisher
  8. D Avino P, Savoian M, Glover D. Cleavage furrow formation and ingression during animal cytokinesis: a microtubule legacy. J Cell Sci. 2005;118:1549-58 pubmed
    ..The same mechanism might be conserved in other organisms. ..
  9. Giansanti M, Farkas R, Bonaccorsi S, Lindsley D, Wakimoto B, Fuller M, et al. Genetic dissection of meiotic cytokinesis in Drosophila males. Mol Biol Cell. 2004;15:2509-22 pubmed
    ..These include 16 novel loci and three genes, diaphanous, four wheel drive, and pebble, already known to be involved in Drosophila cytokinesis...

More Information

Publications68

  1. Acharya S, Laupsien P, Wenzl C, Yan S, Großhans J. Function and dynamics of slam in furrow formation in early Drosophila embryo. Dev Biol. 2014;386:371-84 pubmed publisher
    ..Following the onset of zygotic gene expression, Slam and Nullo together stabilise this mark and Slam triggers invagination of the cleavage furrow. ..
  2. Peng Y, Han C, Axelrod J. Planar polarized protrusions break the symmetry of EGFR signaling during Drosophila bract cell fate induction. Dev Cell. 2012;23:507-18 pubmed publisher
    ..The planar polarized direction of the protrusions determines the direction of the signaling outcome. This asymmetric cell signaling serves as a developmental mechanism to generate spatially patterned cell fates. ..
  3. Eggert U, Kiger A, Richter C, Perlman Z, Perrimon N, Mitchison T, et al. Parallel chemical genetic and genome-wide RNAi screens identify cytokinesis inhibitors and targets. PLoS Biol. 2004;2:e379 pubmed
    ..Our study shows that parallel RNA interference and small molecule screening is a generally useful approach to identifying active small molecules and their target pathways...
  4. Hickson G, O Farrell P. Rho-dependent control of anillin behavior during cytokinesis. J Cell Biol. 2008;180:285-94 pubmed publisher
    ..We propose that such interactions occur dynamically and transiently to promote furrow stability. ..
  5. Afshar K, Stuart B, Wasserman S. Functional analysis of the Drosophila diaphanous FH protein in early embryonic development. Development. 2000;127:1887-97 pubmed
    The Drosophila Formin Homology (FH) protein Diaphanous has an essential role during cytokinesis...
  6. Wang Y, Riechmann V. The role of the actomyosin cytoskeleton in coordination of tissue growth during Drosophila oogenesis. Curr Biol. 2007;17:1349-55 pubmed
    ..Myosin is activated at the apical cortex by localized Rho kinase and inhibited at the basolateral cortex by PP1beta9C. In addition, our data indicate that active myosin is apically anchored by the Baz/Par-6/aPKC complex. ..
  7. Founounou N, Loyer N, Le Borgne R. Septins regulate the contractility of the actomyosin ring to enable adherens junction remodeling during cytokinesis of epithelial cells. Dev Cell. 2013;24:242-55 pubmed publisher
    ..By modulating the strength of tension induced by neighbors, we uncover a mechanical function for septins to overcome the extrinsic tension induced by neighboring interphasic cells. ..
  8. Janody F, Treisman J. Actin capping protein alpha maintains vestigial-expressing cells within the Drosophila wing disc epithelium. Development. 2006;133:3349-57 pubmed
    ..Our results suggest that Vestigial specifies the cytoskeletal changes that lead to morphogenesis of the adult wing. ..
  9. Somogyi K, Rørth P. Evidence for tension-based regulation of Drosophila MAL and SRF during invasive cell migration. Dev Cell. 2004;7:85-93 pubmed
    ..Like mammalian MAL, MAL-D also responds to activated Diaphanous, which affects actin dynamics...
  10. Padash Barmchi M, Rogers S, Hacker U. DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo. J Cell Biol. 2005;168:575-85 pubmed
    ..Our results support the hypothesis that specific aspects of Rho1 function are regulated by specific GTP exchange factors...
  11. Neisch A, Speck O, Stronach B, Fehon R. Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane. J Cell Biol. 2010;189:311-23 pubmed publisher
    ..Together, these results suggest that Rho1 functions at the cell cortex to regulate JNK activity and implicate Rho1 and Moe in epithelial cell survival. ..
  12. Jaiswal R, Stepanik V, Rankova A, Molinar O, Goode B, McCartney B. Drosophila homologues of adenomatous polyposis coli (APC) and the formin diaphanous collaborate by a conserved mechanism to stimulate actin filament assembly. J Biol Chem. 2013;288:13897-905 pubmed publisher
    Vertebrate APC collaborates with Dia through its Basic domain to assemble actin filaments...
  13. Abreu Blanco M, Verboon J, Parkhurst S. Coordination of Rho family GTPase activities to orchestrate cytoskeleton responses during cell wound repair. Curr Biol. 2014;24:144-55 pubmed publisher
    ..We find Rho1 GTPase uses several downstream effectors, including Diaphanous, Rok, and Pkn, simultaneously to mediate its functions...
  14. Homem C, Peifer M. Diaphanous regulates myosin and adherens junctions to control cell contractility and protrusive behavior during morphogenesis. Development. 2008;135:1005-18 pubmed publisher
    Formins are key regulators of actin nucleation and elongation. Diaphanous-related formins, the best-known subclass, are activated by Rho and play essential roles in cytokinesis...
  15. Ng J, Luo L. Rho GTPases regulate axon growth through convergent and divergent signaling pathways. Neuron. 2004;44:779-93 pubmed
    ..These genetic analyses reveal convergent and divergent pathways from Rho GTPases to the cytoskeleton during axon growth in vivo and suggest that different developmental outcomes could be achieved by biases in pathway selection...
  16. Laplante C, Paul S, Beitel G, Nilson L. Echinoid regulates tracheal morphology and fusion cell fate in Drosophila. Dev Dyn. 2010;239:2509-19 pubmed publisher
    ..Tracheal-specific expression of Ed rescues these fusion defects, indicating that Ed acts in trachea to control fusion cell fate. ..
  17. Homem C, Peifer M. Exploring the roles of diaphanous and enabled activity in shaping the balance between filopodia and lamellipodia. Mol Biol Cell. 2009;20:5138-55 pubmed publisher
    ..Here we use Drosophila leading-edge (LE) cells to explore how Diaphanous (Dia)-related formins and Ena/VASP proteins cooperate in this process...
  18. Prokopenko S, Saint R, Bellen H. Untying the Gordian knot of cytokinesis. Role of small G proteins and their regulators. J Cell Biol. 2000;148:843-8 pubmed
  19. Lecuit T. Junctions and vesicular trafficking during Drosophila cellularization. J Cell Sci. 2004;117:3427-33 pubmed
    ..The novel proteins Nullo and Slam, which are specifically induced during cellularization, represent developmental regulators of membrane growth during cellularization. ..
  20. Yan S, Lv Z, Winterhoff M, Wenzl C, Zobel T, Faix J, et al. The F-BAR protein Cip4/Toca-1 antagonizes the formin Diaphanous in membrane stabilization and compartmentalization. J Cell Sci. 2013;126:1796-805 pubmed publisher
    ..The actin nucleator mediating this function is unknown. We found that the formin Diaphanous (Dia) is required for establishing and maintaining distinct lateral and basal domains during cellularization...
  21. Lee D, Harris T. An Arf-GEF regulates antagonism between endocytosis and the cytoskeleton for Drosophila blastoderm development. Curr Biol. 2013;23:2110-20 pubmed publisher
    ..Endocytosis also regulates the PM and can be promoted or inhibited by cytoskeletal networks. However, endocytic regulation of the general membrane cytoskeleton is undocumented...
  22. Matusek T, Djiane A, Jankovics F, Brunner D, Mlodzik M, Mihaly J. The Drosophila formin DAAM regulates the tracheal cuticle pattern through organizing the actin cytoskeleton. Development. 2006;133:957-66 pubmed
  23. Delon I, Brown N. The integrin adhesion complex changes its composition and function during morphogenesis of an epithelium. J Cell Sci. 2009;122:4363-74 pubmed publisher
    ..Early expression of alphaPS1betaPS integrin decreases the levels of the actin-nucleating factors Enabled, Diaphanous and profilin, as well as downregulating the amount of F-actin incorporated into the stress fibres...
  24. Bertet C, Rauzi M, Lecuit T. Repression of Wasp by JAK/STAT signalling inhibits medial actomyosin network assembly and apical cell constriction in intercalating epithelial cells. Development. 2009;136:4199-212 pubmed publisher
    ..We show that regulation of actin polymerization by Wasp, but not by Dia, is important in this process...
  25. Roy S, Huang H, Liu S, Kornberg T. Cytoneme-mediated contact-dependent transport of the Drosophila decapentaplegic signaling protein. Science. 2014;343:1244624 pubmed publisher
    ..Genetic loss-of-function conditions for diaphanous, shibire, neuroglian, and capricious perturbed cytonemes by reducing their number or only the synapses they make ..
  26. Johnston C, Manning L, Lu M, Golub O, Doe C, Prehoda K. Formin-mediated actin polymerization cooperates with Mushroom body defect (Mud)-Dynein during Frizzled-Dishevelled spindle orientation. J Cell Sci. 2013;126:4436-44 pubmed publisher
    ..The formin Diaphanous (Dia) functions as the Rho effector in this pathway, inducing F-actin enrichment at sites of cortical Dsh...
  27. Cao J, Albertson R, Riggs B, Field C, Sullivan W. Nuf, a Rab11 effector, maintains cytokinetic furrow integrity by promoting local actin polymerization. J Cell Biol. 2008;182:301-13 pubmed publisher
    ..We propose a model in which RE-derived vesicles promote furrow integrity by regulating the rate of actin polymerization through the RhoGEF2-Rho1 pathway. ..
  28. Somers W, Saint R. A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis. Dev Cell. 2003;4:29-39 pubmed
  29. Dean S, Rogers S, Stuurman N, Vale R, Spudich J. Distinct pathways control recruitment and maintenance of myosin II at the cleavage furrow during cytokinesis. Proc Natl Acad Sci U S A. 2005;102:13473-8 pubmed
    ..However, F-actin, the centralspindlin complex, formin (diaphanous), and profilin (chickadee) are required to stably maintain myosin II at the furrow...
  30. Mulinari S, Barmchi M, Hacker U. DRhoGEF2 and diaphanous regulate contractile force during segmental groove morphogenesis in the Drosophila embryo. Mol Biol Cell. 2008;19:1883-92 pubmed publisher
    ..We further show that the Formin Diaphanous is required for groove formation and strengthens cell junctions in the epidermis...
  31. Antunes M, Pereira T, Cordeiro J, Almeida L, Jacinto A. Coordinated waves of actomyosin flow and apical cell constriction immediately after wounding. J Cell Biol. 2013;202:365-79 pubmed publisher
    ..We show that this actomyosin flow was regulated by Diaphanous and ROCK and that it elicited a wave of apical cell constriction that culminated in the formation of the leading ..
  32. Verdier V, Guang Chao Chen -, Settleman J. Rho-kinase regulates tissue morphogenesis via non-muscle myosin and LIM-kinase during Drosophila development. BMC Dev Biol. 2006;6:38 pubmed
    ..downstream effectors of the Rho GTPase signaling pathway, including non-muscle myosin heavy chain, adducin, and Diaphanous in those developmental processes...
  33. Mason F, Tworoger M, Martin A. Apical domain polarization localizes actin-myosin activity to drive ratchet-like apical constriction. Nat Cell Biol. 2013;15:926-36 pubmed publisher
    ..The formin Diaphanous mediates apical actin assembly to suppress medioapical E-cadherin localization and form stable connections ..
  34. Hickson G, Echard A, O Farrell P. Rho-kinase controls cell shape changes during cytokinesis. Curr Biol. 2006;16:359-70 pubmed
    ..to Rok depletion was highlighted by RNAi to other genes in the Rho pathway, such as pebble, racGAP50C, and diaphanous, which had profound effects on furrowing but lesser effects on elongation...
  35. Prokopenko S, Brumby A, O KEEFE L, Prior L, He Y, Saint R, et al. A putative exchange factor for Rho1 GTPase is required for initiation of cytokinesis in Drosophila. Genes Dev. 1999;13:2301-14 pubmed
    ..Our results identify Pbl as a RhoGEF specifically required for cytokinesis and linked through Rho1 activity to the reorganization of the actin cytoskeleton at the cleavage furrow. ..
  36. Tucker P, Evans I, Wood W. Ena drives invasive macrophage migration in Drosophila embryos. Dis Model Mech. 2011;4:126-34 pubmed publisher
  37. Levayer R, Pelissier Monier A, Lecuit T. Spatial regulation of Dia and Myosin-II by RhoGEF2 controls initiation of E-cadherin endocytosis during epithelial morphogenesis. Nat Cell Biol. 2011;13:529-40 pubmed publisher
    ..This requires the concerted action of the formin Diaphanous (Dia) and Myosin-II...
  38. Giansanti M, Bonaccorsi S, Williams B, Williams E, Santolamazza C, Goldberg M, et al. Cooperative interactions between the central spindle and the contractile ring during Drosophila cytokinesis. Genes Dev. 1998;12:396-410 pubmed
    ..the central spindle and the contractile ring, we examined meiosis in the cytokinesis-defective mutants KLP3A and diaphanous and in testes treated with cytochalasin B...
  39. Warner S, Longmore G. Distinct functions for Rho1 in maintaining adherens junctions and apical tension in remodeling epithelia. J Cell Biol. 2009;185:1111-25 pubmed publisher
    ..Surprisingly, neither of the Rho effectors, Rok or Dia, is necessary downstream of Rho1 to maintain AJs; instead, Rho1 maintains AJs by inhibiting Drosophila epithelial ..
  40. Pawson C, Eaton B, Davis G. Formin-dependent synaptic growth: evidence that Dlar signals via Diaphanous to modulate synaptic actin and dynamic pioneer microtubules. J Neurosci. 2008;28:11111-23 pubmed publisher
    The diaphanous gene is the founding member of a family of Diaphanous-related formin proteins (DRFs)...
  41. Massarwa R, Schejter E, Shilo B. Apical secretion in epithelial tubes of the Drosophila embryo is directed by the Formin-family protein Diaphanous. Dev Cell. 2009;16:877-88 pubmed publisher
    ..However, its origins and function are not known. We demonstrate that the Diaphanous (Dia)/Formin actin-nucleating factor is required for generation of apical F-actin in diverse types of epithelial ..
  42. Zavortink M, Contreras N, Addy T, Bejsovec A, Saint R. Tum/RacGAP50C provides a critical link between anaphase microtubules and the assembly of the contractile ring in Drosophila melanogaster. J Cell Sci. 2005;118:5381-92 pubmed
    ..Tum do not form furrows and fail to localise the key cytokinetic components Pebble (a RhoGEF), Aurora B kinase, Diaphanous, Pav-KLP and Anillin...
  43. Chen G, Gajowniczek P, Settleman J. Rho-LIM kinase signaling regulates ecdysone-induced gene expression and morphogenesis during Drosophila metamorphosis. Curr Biol. 2004;14:309-13 pubmed
    ..Together, these findings indicate a link between Rho-LIMK signaling and steroid hormone-induced gene expression in the context of metamorphosis and thereby establish a novel role for the Rho GTPase in development. ..
  44. Grevengoed E, Fox D, Gates J, Peifer M. Balancing different types of actin polymerization at distinct sites: roles for Abelson kinase and Enabled. J Cell Biol. 2003;163:1267-79 pubmed
    ..We also examined other actin regulators. Loss of Abl leads to changes in the localization of the Arp2/3 complex and the formin Diaphanous, and mutations in diaphanous or capping protein beta enhance abl phenotypes.
  45. Castrillon D, Wasserman S. Diaphanous is required for cytokinesis in Drosophila and shares domains of similarity with the products of the limb deformity gene. Development. 1994;120:3367-77 pubmed
    We show that the Drosophila gene diaphanous is required for cytokinesis. Males homozygous for the dia1 mutation are sterile due to a defect in cytokinesis in the germline...
  46. Davis J, Luchici A, Mosis F, Thackery J, Salazar J, Mao Y, et al. Inter-cellular forces orchestrate contact inhibition of locomotion. Cell. 2015;161:361-73 pubmed publisher
    ..Our findings reveal that the physical coupling of the flowing actin networks during CIL acts as a mechanotransducer, allowing cells to haptically sense each other and coordinate their behaviors. ..
  47. Levayer R, Hauert B, Moreno E. Cell mixing induced by myc is required for competitive tissue invasion and destruction. Nature. 2015;524:476-80 pubmed publisher
    ..Our results establish the first link between cell-cell intercalation induced by a proto-oncogene and how it promotes invasiveness and destruction of healthy tissues. ..
  48. Zeller R, Haramis A, Zuniga A, McGuigan C, Dono R, Davidson G, et al. Formin defines a large family of morphoregulatory genes and functions in establishment of the polarising region. Cell Tissue Res. 1999;296:85-93 pubmed
  49. Haralalka S, Shelton C, Cartwright H, Guo F, Trimble R, Kumar R, et al. Live imaging provides new insights on dynamic F-actin filopodia and differential endocytosis during myoblast fusion in Drosophila. PLoS ONE. 2014;9:e114126 pubmed publisher
    ..Consistent with this, formin Diaphanous (Dia) and branched actin nucleator, Arp3, are found decorating the filopodia or enriched at the actin focus, ..
  50. Rikhy R, Mavrakis M, Lippincott Schwartz J. Dynamin regulates metaphase furrow formation and plasma membrane compartmentalization in the syncytial Drosophila embryo. Biol Open. 2015;4:301-11 pubmed publisher
    ..Based on these findings, we propose that cell cycle changes in dynamin orchestrate recruitment of actin regulatory machinery for PM furrow dynamics during the early mitotic cycles in the Drosophila embryo. ..
  51. Tsygankov D, Bilancia C, Vitriol E, Hahn K, Peifer M, Elston T. CellGeo: a computational platform for the analysis of shape changes in cells with complex geometries. J Cell Biol. 2014;204:443-60 pubmed publisher
    ..We demonstrate CellGeo's utility by deriving new insights into (a) the roles of Diaphanous, Enabled, and Capping protein in regulating filopodia and lamellipodia dynamics in Drosophila melanogaster cells ..
  52. Vogler G, Liu J, Iafe T, Migh E, Mihály J, Bodmer R. Cdc42 and formin activity control non-muscle myosin dynamics during Drosophila heart morphogenesis. J Cell Biol. 2014;206:909-22 pubmed publisher
    ..Heart-specific expression of activated Cdc42, or the regulatory formins dDAAM and Diaphanous caused mislocalization of Zipper and induced ectopic heart lumina, as characterized by luminal markers such as ..
  53. Kollmar R. Who does the hair cell's 'do? Rho GTPases and hair-bundle morphogenesis. Curr Opin Neurobiol. 1999;9:394-8 pubmed
  54. Rousso T, Schejter E, Shilo B. Orchestrated content release from Drosophila glue-protein vesicles by a contractile actomyosin network. Nat Cell Biol. 2016;18:181-90 pubmed publisher
    ..We identify the Formin family protein Diaphanous (Dia) as the main actin nucleator involved in generating this structure, and uncover Rho as an integrator of ..
  55. Milan M, Clemente Ruiz M, Dekanty A, Muzzopappa M. Aneuploidy and tumorigenesis in Drosophila. Semin Cell Dev Biol. 2014;28:110-5 pubmed publisher
    ..Here we discuss the role of aneuploidy in tumorigenesis in light of the contribution of Drosophila epithelial cancer models and propose a stress-induced tumor-promoting role of aneuploidy. ..
  56. Kadandale P, Stender J, Glass C, Kiger A. Conserved role for autophagy in Rho1-mediated cortical remodeling and blood cell recruitment. Proc Natl Acad Sci U S A. 2010;107:10502-7 pubmed publisher
    ..These results illuminate a specific and conserved role for autophagy as a regulatory mechanism for cortical remodeling, with implications for immune cell function. ..
  57. Llense F, Martin Blanco E. JNK signaling controls border cell cluster integrity and collective cell migration. Curr Biol. 2008;18:538-44 pubmed publisher
    ..We anticipate a role for the JNK pathway in controlling collective cell movements in other morphogenetic and clinical models. ..
  58. Webb R, Zhou M, McCartney B. A novel role for an APC2-Diaphanous complex in regulating actin organization in Drosophila. Development. 2009;136:1283-93 pubmed publisher
    ..Furthermore, we show that the formin Diaphanous (DIA) functions with APC2 in this process...
  59. Cao J, Crest J, Fasulo B, Sullivan W. Cortical actin dynamics facilitate early-stage centrosome separation. Curr Biol. 2010;20:770-6 pubmed publisher