Dcr-1

Summary

Gene Symbol: Dcr-1
Description: Dicer-1
Alias: CG4792, DCR-1, DCR1, DICER, Dcr, Dcr1, Dicer, Dicer1, Dmel\CG4792, cg4792, dcr 1, dcr-1, dcr1, dicer, dicer-1, dicer1, dmDcr1, Dicer-1, CG4792-PA, Dcr-1-PA, dicer, dicer 1, dicer1
Species: fruit fly
Products:     Dcr-1

Top Publications

  1. Flynt A, Greimann J, Chung W, Lima C, Lai E. MicroRNA biogenesis via splicing and exosome-mediated trimming in Drosophila. Mol Cell. 2010;38:900-7 pubmed publisher
    ..Analysis of cloned small RNAs yielded six additional candidate 3' tailed mirtrons in D. melanogaster. Altogether, these data reveal an unexpected role for the exosome in the biogenesis of miRNAs from hybrid mirtron substrates. ..
  2. Miyoshi K, Miyoshi T, Hartig J, Siomi H, Siomi M. Molecular mechanisms that funnel RNA precursors into endogenous small-interfering RNA and microRNA biogenesis pathways in Drosophila. RNA. 2010;16:506-15 pubmed publisher
    ..Although piRNAs are produced independently of Dicer, miRNA and esiRNA biogenesis pathways require Dicer1 and Dicer2, respectively...
  3. Okamura K, Chung W, Ruby J, Guo H, Bartel D, Lai E. The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs. Nature. 2008;453:803-6 pubmed publisher
    ..This notion is based on the fact that mutants lacking the core siRNA-generating enzyme Dicer-2 or the predominant siRNA effector Argonaute 2 are viable, fertile and of relatively normal morphology...
  4. Vagin V, Sigova A, Li C, Seitz H, Gvozdev V, Zamore P. A distinct small RNA pathway silences selfish genetic elements in the germline. Science. 2006;313:320-4 pubmed
    ..rasiRNA production appears not to require Dicer-1, which makes microRNAs (miRNAs), or Dicer-2, which makes siRNAs, and rasiRNAs lack the 2',3' hydroxy termini ..
  5. Hartig J, Esslinger S, Böttcher R, Saito K, Forstemann K. Endo-siRNAs depend on a new isoform of loquacious and target artificially introduced, high-copy sequences. EMBO J. 2009;28:2932-44 pubmed publisher
    ..One strand of the dsRNA precursor can come from spliced mRNA, whereas the opposite strand derives from independent transcripts in antisense orientation. ..
  6. Ishizuka A, Siomi M, Siomi H. A Drosophila fragile X protein interacts with components of RNAi and ribosomal proteins. Genes Dev. 2002;16:2497-508 pubmed
    ..We show that Dmp68 is also required for efficient RNAi. We further show that dFMR1 is associated with Dicer, another essential component of the RNAi pathway, and microRNAs (miRNAs) in vivo, suggesting that dFMR1 is part of ..
  7. Czech B, Zhou R, Erlich Y, Brennecke J, Binari R, Villalta C, et al. Hierarchical rules for Argonaute loading in Drosophila. Mol Cell. 2009;36:445-56 pubmed publisher
  8. Herranz H, Hong X, Perez L, Ferreira A, Olivieri D, Cohen S, et al. The miRNA machinery targets Mei-P26 and regulates Myc protein levels in the Drosophila wing. EMBO J. 2010;29:1688-98 pubmed publisher
    ..b>Dicer-1 (Dcr-1) is a double-stranded RNAseIII essential for miRNA biogenesis...
  9. Hammond S, Boettcher S, Caudy A, Kobayashi R, Hannon G. Argonaute2, a link between genetic and biochemical analyses of RNAi. Science. 2001;293:1146-50 pubmed
    ..This observation begins the process of forging links between genetic analysis of RNAi from diverse organisms and the biochemical model of RNAi that is emerging from Drosophila in vitro systems. ..

More Information

Publications65

  1. Chung W, Okamura K, Martin R, Lai E. Endogenous RNA interference provides a somatic defense against Drosophila transposons. Curr Biol. 2008;18:795-802 pubmed publisher
    ..endogenous small interfering RNAs (siRNAs), which are 21 nucleotide, 3'-end-modified RNAs that are dependent on Dicer-2 and Argonaute-2...
  2. Tomari Y, Zamore P. Perspective: machines for RNAi. Genes Dev. 2005;19:517-29 pubmed
    ..Here, we review our current understanding of how small RNAs are produced, how they are loaded into protein complexes, and how they repress gene expression. ..
  3. Okamura K, Ishizuka A, Siomi H, Siomi M. Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways. Genes Dev. 2004;18:1655-66 pubmed
    ..The association of AGO1 with Dicer-1 and pre-miRNA also suggests that AGO1 is involved in miRNA biogenesis...
  4. Lee Y, Nakahara K, Pham J, Kim K, He Z, Sontheimer E, et al. Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. Cell. 2004;117:69-81 pubmed
    The RNase III enzyme Dicer processes RNA into siRNAs and miRNAs, which direct a RNA-induced silencing complex (RISC) to cleave mRNA or block its translation (RNAi)...
  5. Eulalio A, Rehwinkel J, Stricker M, Huntzinger E, Yang S, Doerks T, et al. Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing. Genes Dev. 2007;21:2558-70 pubmed
    ..In addition to proteins known to be required for miRNA biogenesis and function (i.e., Drosha, Pasha, Dicer-1, AGO1, and GW182), the screen identified the decapping activator Ge-1 as being required for silencing by miRNAs...
  6. Okamura K, Robine N, Liu Y, Liu Q, Lai E. R2D2 organizes small regulatory RNA pathways in Drosophila. Mol Cell Biol. 2011;31:884-96 pubmed publisher
    Drosophila microRNAs (miRNAs) and small interfering RNAs (siRNAs) are generally produced by different Dicer enzymes (Dcr-1 and Dcr-2) and sorted to functionally distinct Argonaute effectors (AGO1 and AGO2)...
  7. Ruby J, Jan C, Bartel D. Intronic microRNA precursors that bypass Drosha processing. Nature. 2007;448:83-6 pubmed
    ..Drosha cleaves near the base of the stem to liberate a approximately 60-nucleotide pre-miRNA hairpin, then Dicer cleaves near the loop to generate a miRNA:miRNA* duplex...
  8. Miyoshi K, Okada T, Siomi H, Siomi M. Characterization of the miRNA-RISC loading complex and miRNA-RISC formed in the Drosophila miRNA pathway. RNA. 2009;15:1282-91 pubmed publisher
    In Drosophila, miRNA is processed by Dicer-1 (DCR-1) from its precursor and loaded onto Argonaute1 (AGO1). AGO1 recognizes target mRNAs based on the miRNA sequence and suppresses the expression at post-transcriptional levels...
  9. Ghildiyal M, Seitz H, Horwich M, Li C, Du T, Lee S, et al. Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells. Science. 2008;320:1077-81 pubmed publisher
    ..Normal accumulation of somatic endo-siRNAs requires the siRNA-generating ribonuclease Dicer-2 and the RNAi effector protein Argonaute2 (Ago2)...
  10. Bernstein E, Caudy A, Hammond S, Hannon G. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature. 2001;409:363-6 pubmed
    ..Here we identify an enzyme, Dicer, which can produce putative guide RNAs...
  11. Xu X, Li Y, Wang F, Gao F. The steady-state level of the nervous-system-specific microRNA-124a is regulated by dFMR1 in Drosophila. J Neurosci. 2008;28:11883-9 pubmed publisher
    ..Indeed, in contrast with the complete loss of mature miR-124a in Dicer-1 mutants, steady-state levels of endogenous or ectopically expressed mature miR-124a were partially reduced in ..
  12. Jing Q, Huang S, Guth S, Zarubin T, Motoyama A, Chen J, et al. Involvement of microRNA in AU-rich element-mediated mRNA instability. Cell. 2005;120:623-34 pubmed
    ..An RNAi-based screen performed in Drosophila S2 cells has revealed that Dicer1, Argonaute1 (Ago1) and Ago2, components involved in microRNA (miRNA) processing and function, are required for the ..
  13. Martin R, Smibert P, Yalcin A, Tyler D, Schäfer U, Tuschl T, et al. A Drosophila pasha mutant distinguishes the canonical microRNA and mirtron pathways. Mol Cell Biol. 2009;29:861-70 pubmed publisher
    ..While pasha adult somatic clones are similar in many respects to those of dicer-1 clones, pasha mutant larvae revealed an unexpected requirement for the miRNA pathway in imaginal disc growth...
  14. Okamura K, Liu N, Lai E. Distinct mechanisms for microRNA strand selection by Drosophila Argonautes. Mol Cell. 2009;36:431-44 pubmed publisher
  15. Schwarz D, Hutvagner G, Haley B, Zamore P. Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways. Mol Cell. 2002;10:537-48 pubmed
    ..We conclude that the underlying mechanism of RNAi is conserved between flies and mammals and that RNA-dependent RNA polymerases are not required for RNAi in these organisms. ..
  16. Flynt A, Liu N, Martin R, Lai E. Dicing of viral replication intermediates during silencing of latent Drosophila viruses. Proc Natl Acad Sci U S A. 2009;106:5270-5 pubmed publisher
    ..Instead, this + strand virus specifically generated Dicer-2-dependent, 21-nucleotide siRNAs that derived in equal proportion from + and - strands...
  17. Saito K, Ishizuka A, Siomi H, Siomi M. Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells. PLoS Biol. 2005;3:e235 pubmed
    ..These pre-miRNAs are then exported to the cytoplasm and further processed by Dicer to mature miRNAs...
  18. Pham J, Pellino J, Lee Y, Carthew R, Sontheimer E. A Dicer-2-dependent 80s complex cleaves targeted mRNAs during RNAi in Drosophila. Cell. 2004;117:83-94 pubmed
    ..To form, all three complexes require Dicer-2 (Dcr-2), which directly contacts siRNAs in the ATP-independent R1 complex...
  19. Lim A, Kai T. Unique germ-line organelle, nuage, functions to repress selfish genetic elements in Drosophila melanogaster. Proc Natl Acad Sci U S A. 2007;104:6714-9 pubmed
    ..Hence, our data suggest that the nuage functions as a specialized center that protects the genome in the germ-line cells via gene regulation mediated by repeat-associated small interfering RNAs. ..
  20. Okamura K, Ladewig E, Zhou L, Lai E. Functional small RNAs are generated from select miRNA hairpin loops in flies and mammals. Genes Dev. 2013;27:778-92 pubmed publisher
    ..animal microRNA (miRNA) pathway, Drosha generates ?60- to 70-nucleotide pre-miRNA hairpins that are cleaved by Dicer into small RNA duplexes that load into Argonaute proteins, which retain a single mature strand in the active ..
  21. Sabin L, Zhou R, Gruber J, Lukinova N, Bambina S, Berman A, et al. Ars2 regulates both miRNA- and siRNA- dependent silencing and suppresses RNA virus infection in Drosophila. Cell. 2009;138:340-51 pubmed publisher
    ..Consistent with its antiviral properties, we found that Ars2 physically interacts with Dcr-2, modulates its activity in vitro, and is required for siRNA-mediated silencing...
  22. Tomari Y, Du T, Zamore P. Sorting of Drosophila small silencing RNAs. Cell. 2007;130:299-308 pubmed
    ..We find that the Dcr-2/R2D2 heterodimer acts as a gatekeeper for the assembly of Ago2 complexes, promoting the incorporation of siRNAs ..
  23. Li H, Li W, Ding S. Induction and suppression of RNA silencing by an animal virus. Science. 2002;296:1319-21 pubmed
    ..These findings establish RNA silencing as an adaptive antiviral defense in animal cells. B2 also inhibits RNA silencing in transgenic plants, providing evidence for a conserved RNA silencing pathway in the plant and animal kingdoms...
  24. Kavi H, Fernandez H, Xie W, Birchler J. RNA silencing in Drosophila. FEBS Lett. 2005;579:5940-9 pubmed
    ..RNA silencing processes in Drosophila are described. ..
  25. Du T, Zamore P. microPrimer: the biogenesis and function of microRNA. Development. 2005;132:4645-52 pubmed
    ..miRNAs function very much like siRNAs, but these two types of small RNAs can be distinguished by their distinct pathways for maturation and by the logic by which they regulate gene expression. ..
  26. Gangaraju V, Yin H, Weiner M, Wang J, Huang X, Lin H. Drosophila Piwi functions in Hsp90-mediated suppression of phenotypic variation. Nat Genet. 2011;43:153-8 pubmed publisher
    ..Moreover, they point to epigenetic silencing of the expression of existing genetic variants and the suppression of transposon-induced new genetic variation as two major mechanisms underlying piRNA pathway-mediated canalization. ..
  27. Westholm J, Ladewig E, Okamura K, Robine N, Lai E. Common and distinct patterns of terminal modifications to mirtrons and canonical microRNAs. RNA. 2012;18:177-92 pubmed publisher
    ..Finally, we found that 3' resection occurs broadly across AGO1-loaded canonical miRNA and star species. Altogether, these findings substantially broaden the complexity of terminal modification pathways acting upon small regulatory RNAs. ..
  28. Yu Z, Teng X, Bonini N. Triplet repeat-derived siRNAs enhance RNA-mediated toxicity in a Drosophila model for myotonic dystrophy. PLoS Genet. 2011;7:e1001340 pubmed publisher
    ..Expression of the two transcripts led to novel pathogenesis with the generation of dcr-2 and ago2-dependent 21-nt triplet repeat-derived siRNAs...
  29. Han B, Hung J, Weng Z, Zamore P, Ameres S. The 3'-to-5' exoribonuclease Nibbler shapes the 3' ends of microRNAs bound to Drosophila Argonaute1. Curr Biol. 2011;21:1878-87 pubmed publisher
    ..of primary hairpin-containing RNA polymerase II transcripts by the RNase III enzymes Drosha in the nucleus and Dicer in the cytoplasm...
  30. Jiang F, Ye X, Liu X, Fincher L, McKearin D, Liu Q. Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila. Genes Dev. 2005;19:1674-9 pubmed
    In Drosophila melanogaster, Dicer-2/R2D2 and Dicer-1 generate small interfering RNA (siRNA) and microRNA (miRNA), respectively...
  31. Fukunaga R, Han B, Hung J, Xu J, Weng Z, Zamore P. Dicer partner proteins tune the length of mature miRNAs in flies and mammals. Cell. 2012;151:533-46 pubmed publisher
    Drosophila Dicer-1 produces microRNAs (miRNAs) from pre-miRNA, whereas Dicer-2 generates small interfering RNAs (siRNAs) from long dsRNA. Alternative splicing of the loquacious (loqs) mRNA generates three distinct Dicer partner proteins...
  32. Zhou R, Czech B, Brennecke J, Sachidanandam R, Wohlschlegel J, Perrimon N, et al. Processing of Drosophila endo-siRNAs depends on a specific Loquacious isoform. RNA. 2009;15:1886-95 pubmed publisher
    ..These approximately 21-nt RNAs are produced by a distinct Dicer, Dcr-2, and do not depend on Drosha/Pasha complexes...
  33. Obbard D, Jiggins F, Halligan D, Little T. Natural selection drives extremely rapid evolution in antiviral RNAi genes. Curr Biol. 2006;16:580-5 pubmed
    ..This is a signature of host-pathogen arms races and implies that the ancient battle between RNA viruses and host antiviral RNAi genes is active and significant in shaping RNAi function. ..
  34. Kawamura Y, Saito K, Kin T, Ono Y, Asai K, Sunohara T, et al. Drosophila endogenous small RNAs bind to Argonaute 2 in somatic cells. Nature. 2008;453:793-7 pubmed publisher
    ..germ line, Piwi-interacting RNAs arise from repetitive intergenic elements including retrotransposons by a Dicer-independent pathway and function through the Piwi subfamily of Argonautes to ensure silencing of retrotransposons...
  35. Findley S, Tamanaha M, Clegg N, Ruohola Baker H. Maelstrom, a Drosophila spindle-class gene, encodes a protein that colocalizes with Vasa and RDE1/AGO1 homolog, Aubergine, in nuage. Development. 2003;130:859-71 pubmed
    ..Furthermore, maelstrom mutant ovaries show mislocalization of two proteins involved in the microRNA and/or RNAi pathways, Dicer and Argonaute2, suggesting a potential connection between nuage and the microRNA-pathway.
  36. Hammond S. Dicing and slicing: the core machinery of the RNA interference pathway. FEBS Lett. 2005;579:5822-9 pubmed
    ..At the heart of the pathway are two ribonuclease machines. The ribonuclease III enzyme Dicer initiates the RNAi pathway by generating the active short interfering RNA trigger...
  37. Cenik E, Fukunaga R, Lu G, Dutcher R, Wang Y, Tanaka Hall T, et al. Phosphate and R2D2 restrict the substrate specificity of Dicer-2, an ATP-driven ribonuclease. Mol Cell. 2011;42:172-84 pubmed publisher
    Drosophila Dicer-2 generates small interfering RNAs (siRNAs) from long double-stranded RNA (dsRNA), whereas Dicer-1 produces microRNAs (miRNAs) from pre-miRNA...
  38. Lin H. piRNAs in the germ line. Science. 2007;316:397 pubmed
    ..Recently, a class of small RNAs that interact with Piwi proteins has been discovered in the mammalian germ line and Drosophila. These Piwi-interacting RNAs (piRNAs) represent a distinct small RNA pathway. ..
  39. He L, Hannon G. MicroRNAs: small RNAs with a big role in gene regulation. Nat Rev Genet. 2004;5:522-31 pubmed
  40. Pressman S, Reinke C, Wang X, Carthew R. A Systematic Genetic Screen to Dissect the MicroRNA Pathway in Drosophila. G3 (Bethesda). 2012;2:437-48 pubmed publisher
    ..These include 4 previously known genes that encode core components of the miRNA pathway, including Drosha, Pasha, Dicer-1, and Ago1. The rest are new genes that function through chromatin remodeling, signaling, and mRNA decapping...
  41. Forstemann K, Horwich M, Wee L, Tomari Y, Zamore P. Drosophila microRNAs are sorted into functionally distinct argonaute complexes after production by dicer-1. Cell. 2007;130:287-97 pubmed
    ..Thus, the two members of the Drosophila Ago subclade of Argonaute proteins are functionally specialized, but specific small RNA classes are not restricted to associate with Ago1 or Ago2. ..
  42. Smibert P, Bejarano F, Wang D, Garaulet D, Yang J, Martin R, et al. A Drosophila genetic screen yields allelic series of core microRNA biogenesis factors and reveals post-developmental roles for microRNAs. RNA. 2011;17:1997-2010 pubmed publisher
    ..are ?22-nt regulatory RNAs generated by stepwise cleavage of primary hairpin transcripts by the Drosha and Dicer RNase III enzymes...
  43. Ghildiyal M, Xu J, Seitz H, Weng Z, Zamore P. Sorting of Drosophila small silencing RNAs partitions microRNA* strands into the RNA interference pathway. RNA. 2010;16:43-56 pubmed publisher
    ..Central to this sorting decision is the affinity of the small RNA duplex for the Dcr-2/R2D2 heterodimer, which loads small RNAs into Ago2...
  44. Jin Z, Xie T. Dcr-1 maintains Drosophila ovarian stem cells. Curr Biol. 2007;17:539-44 pubmed
    ..In Drosophila, Dicer-1 (Dcr-1) is essential for generating mature miRNAs from their corresponding precursors...
  45. Smibert P, Yang J, Azzam G, Liu J, Lai E. Homeostatic control of Argonaute stability by microRNA availability. Nat Struct Mol Biol. 2013;20:789-95 pubmed publisher
    ..In mouse cells, we found that the stability of Ago2 declined in Dicer-knockout cells and was rescued by proteasome blockade or introduction of either Dicer plasmid or Dicer-independent ..
  46. Landthaler M, Yalcin A, Tuschl T. The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis. Curr Biol. 2004;14:2162-7 pubmed
    ..a hairpin RNA (pre-miRNA), which is followed by export of the pre-miRNA to the cytoplasm and further processing by Dicer to mature miRNAs...
  47. Forstemann K, Tomari Y, Du T, Vagin V, Denli A, Bratu D, et al. Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain protein. PLoS Biol. 2005;3:e236 pubmed
    ..Drosha converts pri-miRNA transcripts to precursor miRNA (pre-miRNA); Dicer, in turn, converts pre-miRNA to mature miRNA...
  48. Meyer W, Schreiber S, Guo Y, Volkmann T, Welte M, Müller H. Overlapping functions of argonaute proteins in patterning and morphogenesis of Drosophila embryos. PLoS Genet. 2006;2:e134 pubmed
    ..Our findings argue against a strict separation of Ago1 and Ago2 functions and suggest that these proteins act in concert to control key steps of the midblastula transition and of segmental patterning. ..
  49. Tomari Y, Zamore P. MicroRNA biogenesis: drosha can't cut it without a partner. Curr Biol. 2005;15:R61-4 pubmed
  50. Ni J, Zhou R, Czech B, Liu L, Holderbaum L, Yang Zhou D, et al. A genome-scale shRNA resource for transgenic RNAi in Drosophila. Nat Methods. 2011;8:405-7 pubmed publisher
    ..We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource. ..
  51. Liu Q, Rand T, Kalidas S, Du F, Kim H, Smith D, et al. R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway. Science. 2003;301:1921-5 pubmed
    ..The siRNA-generating enzyme was purified from Drosophila S2cells and consists of two stoichiometric subunits: Dicer-2(DCR-2) and a previously unknown protein that we named R2D2...
  52. Kadener S, Rodriguez J, Abruzzi K, Khodor Y, Sugino K, Marr M, et al. Genome-wide identification of targets of the drosha-pasha/DGCR8 complex. RNA. 2009;15:537-45 pubmed publisher
    ..genes, and unlike bona fide pri-miRNAs, they are not affected by depletion of the miRNA processing factor, dicer-1...
  53. Berdnik D, Fan A, Potter C, Luo L. MicroRNA processing pathway regulates olfactory neuron morphogenesis. Curr Biol. 2008;18:1754-9 pubmed publisher
    ..The next cleavage employs the cytoplasmic RNase III Dicer producing miRNA duplexes [3, 4]...
  54. Megosh H, Cox D, Campbell C, Lin H. The role of PIWI and the miRNA machinery in Drosophila germline determination. Curr Biol. 2006;16:1884-94 pubmed
    ..We report the function of Drosophila PIWI, DICER-1, and dFMRP (Fragile X Mental Retardation Protein) in germline determination...
  55. Grimaud C, Bantignies F, Pal Bhadra M, Ghana P, Bhadra U, Cavalli G. RNAi components are required for nuclear clustering of Polycomb group response elements. Cell. 2006;124:957-71 pubmed
    ..b>Dicer-2, PIWI, and Argonaute1, three RNAi components, frequently colocalize with PcG bodies, and their mutation ..
  56. Okamura K, Hagen J, Duan H, Tyler D, Lai E. The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila. Cell. 2007;130:89-100 pubmed
    ..into approximately 22 nucleotide regulatory RNAs via consecutive cleavages by two RNase III enzymes, Drosha and Dicer. In this study, we characterize Drosophila small RNAs that derive from short intronic hairpins termed "..