Gene Symbol: Cyp6a8
Description: Cytochrome P450-6a8
Alias: 6a8, CG10248, CYP6A8, CYP6a8, Cyt-P450-rAF5, Dmel\CG10248, P-450, P450, cytochrome P450-6a8, CG10248-PA, Cyp6a8-PA, cytochrome P450, cytochrome P450 related AF5
Species: fruit fly

Top Publications

  1. Maitra S, Dombrowski S, Waters L, Ganguly R. Three second chromosome-linked clustered Cyp6 genes show differential constitutive and barbital-induced expression in DDT-resistant and susceptible strains of Drosophila melanogaster. Gene. 1996;180:165-71 pubmed
    ..The polypeptides encoded by these two genes, Cyp6a8 and Cyp6a9, show 77 and 75% amino acid sequence similarity, and 60 and 55% identity with Cyp6a2 of D...
  2. Maitra S, Dombrowski S, Basu M, Raustol O, Waters L, Ganguly R. Factors on the third chromosome affect the level of cyp6a2 and cyp6a8 expression in Drosophila melanogaster. Gene. 2000;248:147-56 pubmed
    The expression of two second chromosome-linked cytochrome P450 genes, Cyp6a2 and Cyp6a8, of Drosophila melanogaster was measured in various strains...
  3. Lu B, Zelle K, Seltzer R, Hefetz A, Ben Shahar Y. Feminization of pheromone-sensing neurons affects mating decisions in Drosophila males. Biol Open. 2014;3:152-60 pubmed publisher
    ..Together, these data indicate that the sexual cellular identity of pheromone sensing GRNs plays a major role in how individual flies interpret their social environment in the context of mating decisions. ..
  4. Ffrench Constant R, Daborn P, Le Goff G. The genetics and genomics of insecticide resistance. Trends Genet. 2004;20:163-70 pubmed
    ..These studies enable us to address three fundamental questions in the evolution of resistance. How many genes are involved? How many mutations are there within these genes? How often do these mutations arise in natural populations? ..
  5. Morra R, Kuruganti S, Lam V, Lucchesi J, Ganguly R. Functional analysis of the cis-acting elements responsible for the induction of the Cyp6a8 and Cyp6g1 genes of Drosophila melanogaster by DDT, phenobarbital and caffeine. Insect Mol Biol. 2010;19:121-30 pubmed publisher
    Many Drosophila cytochrome P450 or Cyp genes are induced by caffeine and phenobarbital (PB)...
  6. Chahine S, O Donnell M. Interactions between detoxification mechanisms and excretion in Malpighian tubules of Drosophila melanogaster. J Exp Biol. 2011;214:462-8 pubmed publisher
    ..or synthetic toxins is commonly associated with increases in the activity of detoxification enzymes such as the P450 monoxygenases (P450s) and the glutathione-S-transferases (GSTs)...
  7. Wan H, Liu Y, Li M, Zhu S, Li X, Pittendrigh B, et al. Nrf2/Maf-binding-site-containing functional Cyp6a2 allele is associated with DDT resistance in Drosophila?melanogaster. Pest Manag Sci. 2014;70:1048-58 pubmed publisher
    ..The results suggest that the Nrf2/Maf binding-site-containing functional Cyp6a2?allele is associated with DDT resistance in the D.?melanogaster strains under study. ..
  8. Maitra S, Price C, Ganguly R. Cyp6a8 of Drosophila melanogaster: gene structure, and sequence and functional analysis of the upstream DNA. Insect Biochem Mol Biol. 2002;32:859-70 pubmed overproducer and susceptible 91-C and ry(506) strains are the underproducers of CYP6A8 mRNA encoded by a cytochrome P450 gene, Cyp6a8...
  9. Keogh D, Mitchell M, Crooks J, Smith S. Effects of the adenylate cyclase activator forskolin and its inactive derivative 1,9-dideoxyforskolin on insect cytochrome P-450 dependent steroid hydroxylase activity. Experientia. 1992;48:39-41 pubmed
    ..The concentrations of these labdane diterpenes required to elicit a 50% inhibition of the cytochrome P-450 dependent steroid hydroxylase activity in the insect tissues ranged from approximately 5 x 10(-6) to 5 x 10(-4) M. ..

More Information


  1. Le Goff G, Boundy S, Daborn P, Yen J, Sofer L, Lind R, et al. Microarray analysis of cytochrome P450 mediated insecticide resistance in Drosophila. Insect Biochem Mol Biol. 2003;33:701-8 pubmed
    ..Using microarray analysis of all Drosophila P450 genes, here we show that different P450 genes such as Cyp12d1 and Cyp6a8 can also be selected using DDT in the laboratory...
  2. Helvig C, Tijet N, Feyereisen R, Walker F, Restifo L. Drosophila melanogaster CYP6A8, an insect P450 that catalyzes lauric acid (omega-1)-hydroxylation. Biochem Biophys Res Commun. 2004;325:1495-502 pubmed
    ..b>Cyp6a8 encodes a 506-amino acid protein with 53.6% amino acid identity with CYP6A2...
  3. Deng H, Kerppola T. Visualization of the Drosophila dKeap1-CncC interaction on chromatin illumines cooperative, xenobiotic-specific gene activation. Development. 2014;141:3277-88 pubmed publisher
  4. Kuruganti S, Lam V, Zhou X, Bennett G, Pittendrigh B, Ganguly R. High expression of Cyp6g1, a cytochrome P450 gene, does not necessarily confer DDT resistance in Drosophila melanogaster. Gene. 2007;388:43-53 pubmed
    b>Cytochrome P450 monooxygenases, a family of detoxifying enzymes, are thought to confer resistance to various insecticides including DDT. Daborn et al. [Daborn, P., Yen, J.L., Bogwitz, M., Le Goff, G., Feil, et al. 2002...
  5. Daborn P, Boundy S, Yen J, Pittendrigh B, ffrench Constant R. DDT resistance in Drosophila correlates with Cyp6g1 over-expression and confers cross-resistance to the neonicotinoid imidacloprid. Mol Genet Genomics. 2001;266:556-63 pubmed
    ..The genomic sequence in this interval shows a cluster of cytochrome P450 genes, one of which, Cyp6g1, is over-expressed in all resistant strains examined...
  6. Li T, Cao C, Yang T, Zhang L, He L, Xi Z, et al. A G-protein-coupled receptor regulation pathway in cytochrome P450-mediated permethrin-resistance in mosquitoes, Culex quinquefasciatus. Sci Rep. 2015;5:17772 pubmed publisher
    ..reducing the expression of two cAMP-dependent protein kinase A genes (PKAs) and four resistance related cytochrome P450 genes...
  7. Danielson P, Letman J, Fogleman J. Alkaloid metabolism by cytochrome P-450 enzymes in Drosophila melanogaster. Comp Biochem Physiol B Biochem Mol Biol. 1995;110:683-8 pubmed
    ..Results from larval viability and adult longevity studies indicate that D. melanogaster's in vitro activity does not produce an enhanced in vivo tolerance of alkaloids. ..
  8. Hu J, Jiao D, Xu Q, Ying X, Liu W, Chi Q, et al. Identification of proteasome subunit beta type 2 associated with deltamethrin detoxification in Drosophila Kc cells by cDNA microarray analysis and bioassay analyses. Gene. 2016;582:85-93 pubmed publisher
    ..These detoxification results represent the first evidence that Prosbeta2 plays a role in the detoxification of DM, which may provide new idea and target for studying the molecular mechanisms of insect resistance. ..
  9. Carareto C, Hernandez E, Vieira C. Genomic regions harboring insecticide resistance-associated Cyp genes are enriched by transposable element fragments carrying putative transcription factor binding sites in two sibling Drosophila species. Gene. 2014;537:93-9 pubmed publisher
    ..These fragments carry putative binding sites for transcription factors, which reinforces the hypothesis that DNAREP1 may influence gene regulation and play a role in the adaptation of the Drosophila species. ..
  10. Daborn P, Lumb C, Boey A, Wong W, Ffrench Constant R, Batterham P. Evaluating the insecticide resistance potential of eight Drosophila melanogaster cytochrome P450 genes by transgenic over-expression. Insect Biochem Mol Biol. 2007;37:512-9 pubmed
    ..Drosophila over-expressing Cyp6g1, Cyp6g2, Cyp6t3, Cyp6a2, Cyp6a8, Cyp6a19, Cyp6a23 and Cyp12d1 were screened for resistance to four insecticides--DDT, nitenpyram, dicyclanil and ..
  11. Chen S, Li X. Transposable elements are enriched within or in close proximity to xenobiotic-metabolizing cytochrome P450 genes. BMC Evol Biol. 2007;7:46 pubmed
    ..genes, we scanned two alleles per each of the six xenobiotic-metabolizing Helicoverpa zea cytochrome P450 loci, including CYP6B8, CYP6B27, CYP321A1, CYP321A2, CYP9A12v3 and CYP9A14, for the presence of transposon ..
  12. Willoughby L, Chung H, Lumb C, Robin C, Batterham P, Daborn P. A comparison of Drosophila melanogaster detoxification gene induction responses for six insecticides, caffeine and phenobarbital. Insect Biochem Mol Biol. 2006;36:934-42 pubmed
    ..Mutations leading to changes in expression levels or substrate specificities of cytochrome P450 (P450), glutathione-S-transferase (GST) and esterase genes have been linked to many cases of resistance with ..
  13. Bhaskara S, Dean E, Lam V, Ganguly R. Induction of two cytochrome P450 genes, Cyp6a2 and Cyp6a8, of Drosophila melanogaster by caffeine in adult flies and in cell culture. Gene. 2006;377:56-64 pubmed
    To examine whether caffeine, the most widely used xenobiotic compound, would induce insect cytochrome P450 or CYP gene expression, upstream DNA fragments of Cyp6a2 (0.12, 0.26, 0.52 and 0.98-kb) and Cyp6a8 (0.06, 0.1, 0.2, 0.5 and 0...
  14. Bhaskara S, Chandrasekharan M, Ganguly R. Caffeine induction of Cyp6a2 and Cyp6a8 genes of Drosophila melanogaster is modulated by cAMP and D-JUN protein levels. Gene. 2008;415:49-59 pubmed publisher
    b>Cytochrome P450 monooxygenases or CYPs, a family of endobiotics and xenobiotics metabolizing enzymes, are found in all organisms. We reported earlier that the promoters of Drosophila Cyp6a2 and Cyp6a8 genes are induced by caffeine...
  15. Dombrowski S, Krishnan R, Witte M, Maitra S, Diesing C, Waters L, et al. Constitutive and barbital-induced expression of the Cyp6a2 allele of a high producer strain of CYP6A2 in the genetic background of a low producer strain. Gene. 1998;221:69-77 pubmed
    The levels of one or more cytochrome P450 (CYP) enzymes and the respective mRNAs are found to be higher in insecticide-resistant insects than in susceptible insects...
  16. Zakharenko L, Zakharov I. [Ethanol inhibits recombination in somatic cells of gamma-irradiated larvae of Drosophila melanogaster]. Genetika. 1998;34:364-7 pubmed
    ..In lines with high activity of cytochrome P450, ethanol did not exhibit the radioprotective effect...