ctp

Summary

Gene Symbol: ctp
Description: cut up
Alias: CG6998, Cdlc1, Ctp, DLC8, Ddlc-1, Ddlc1, Dlc, Dmel\CG6998, LC8, anon-WO03040301.128, ddlc1, dlc, l(1)G0153, l(1)G0204, l(1)G0207, l(1)G0244, l(1)G0248, l(1)G0338, l(1)G0371, l(1)G0387, l(1)G0432, l(1)G0445, lincRNA.S8635, cut up, 8kd dynein light chain, CG6998-PA, CG6998-PB, CG6998-PC, CG6998-PE, ctp-PA, ctp-PB, ctp-PC, ctp-PE, cytoplasmic dynein, cytoplasmic dynein 8kD light chain, cytoplasmic dynein light chain 1, dynein, dynein light chain, dynein light chain 1, dynein light chain 8, dynein light chain LC8, dynein light-chain, dynein light-chain 1
Species: fruit fly

Top Publications

  1. Schnorrer F, Bohmann K, Nusslein Volhard C. The molecular motor dynein is involved in targeting swallow and bicoid RNA to the anterior pole of Drosophila oocytes. Nat Cell Biol. 2000;2:185-90 pubmed
    ..We identify dynein light chain-1 (Ddlc-1), a component of the minus-end-directed microtubule motor cytoplasmic dynein, as a Swa-binding ..
  2. Pfister K, Shah P, Hummerich H, Russ A, Cotton J, Annuar A, et al. Genetic analysis of the cytoplasmic dynein subunit families. PLoS Genet. 2006;2:e1 pubmed
    ..relationships among the dynein subunits of mammals and other model organisms, and sheds new light on the role of this diverse group of proteins, highlighting the existence of two cytoplasmic dynein complexes with distinct cellular roles.
  3. Williams J, Roulhac P, Roy A, Vallee R, Fitzgerald M, Hendrickson W. Structural and thermodynamic characterization of a cytoplasmic dynein light chain-intermediate chain complex. Proc Natl Acad Sci U S A. 2007;104:10028-33 pubmed
    b>Cytoplasmic dynein is a microtubule-based motor protein complex that plays important roles in a wide range of fundamental cellular processes, including vesicular transport, mitosis, and cell migration...
  4. Ghosh Roy A, Kulkarni M, Kumar V, Shirolikar S, Ray K. Cytoplasmic dynein-dynactin complex is required for spermatid growth but not axoneme assembly in Drosophila. Mol Biol Cell. 2004;15:2470-83 pubmed
    ..that developing sperm tails were abnormally coiled at the growing ends inside the cysts in the Drosophila Dynein light chain 1 (ddlc1) hemizygous mutant testis...
  5. Nyarko A, Hare M, Hays T, Barbar E. The intermediate chain of cytoplasmic dynein is partially disordered and gains structure upon binding to light-chain LC8. Biochemistry. 2004;43:15595-603 pubmed
    The N-terminal domain of dynein intermediate chain, IC(1-289), is highly disordered, but upon binding to dynein light-chain LC8, it undergoes a significant conformational change to a more ordered structure...
  6. Navarro C, Puthalakath H, Adams J, Strasser A, Lehmann R. Egalitarian binds dynein light chain to establish oocyte polarity and maintain oocyte fate. Nat Cell Biol. 2004;6:427-35 pubmed
    ..Here we report that Egl interacts directly with Drosophila dynein light chain (Dlc), a microtubule motor component, through an Egl domain distinct from that which binds BicD...
  7. Lightcap C, Sun S, Lear J, Rodeck U, Polenova T, Williams J. Biochemical and structural characterization of the Pak1-LC8 interaction. J Biol Chem. 2008;283:27314-24 pubmed publisher
    Pak1 (p21-activated kinase-1) and the dynein light chain, LC8, are overexpressed in breast cancer, and their direct interaction has been proposed to regulate tumor cell survival...
  8. Phillis R, Statton D, Caruccio P, Murphey R. Mutations in the 8 kDa dynein light chain gene disrupt sensory axon projections in the Drosophila imaginal CNS. Development. 1996;122:2955-63 pubmed
    Mutations in an 8 kDa (8x10(3) Mr) cytoplasmic dynein light chain disrupt sensory axon trajectories in the imaginal nervous system of Drosophila...
  9. Benison G, Karplus P, Barbar E. Structure and dynamics of LC8 complexes with KXTQT-motif peptides: swallow and dynein intermediate chain compete for a common site. J Mol Biol. 2007;371:457-68 pubmed
    The dynein light chain LC8 is an integral subunit of the cytoplasmic dynein motor complex that binds directly to and promotes assembly of the dynein intermediate chain (IC)...

More Information

Publications55

  1. Ghosh Roy A, Desai B, Ray K. Dynein light chain 1 regulates dynamin-mediated F-actin assembly during sperm individualization in Drosophila. Mol Biol Cell. 2005;16:3107-16 pubmed
    ..F-actin dynamics plays an important role in this process. Here we report that the dynein light chain 1 (DLC1) of Drosophila is involved in two separate cellular processes during sperm individualization...
  2. Wang L, Hare M, Hays T, Barbar E. Dynein light chain LC8 promotes assembly of the coiled-coil domain of swallow protein. Biochemistry. 2004;43:4611-20 pubmed
    LC8 is a highly conserved light-chain subunit of cytoplasmic dynein that is thought to play a fundamental role in both the assembly of the motor complex and the recruitment of cargo...
  3. Makokha M, Hare M, Li M, Hays T, Barbar E. Interactions of cytoplasmic dynein light chains Tctex-1 and LC8 with the intermediate chain IC74. Biochemistry. 2002;41:4302-11 pubmed
    The interactions of three subunits of cytoplasmic dynein from Drosophila melanogaster, LC8, Tctex-1, and the N-terminal domain of IC74 (N-IC74, residues 1-289), were characterized in vitro by affinity methods, limited proteolysis, and ..
  4. Dick T, Ray K, Salz H, Chia W. Cytoplasmic dynein (ddlc1) mutations cause morphogenetic defects and apoptotic cell death in Drosophila melanogaster. Mol Cell Biol. 1996;16:1966-77 pubmed
    ..Northern blot analysis and epitope tagging show that the hdlc1 gene is ubiquitously expressed and that the human dynein light chain 1 is localized in the cytoplasm. hdlc1 maps to 14q24.
  5. Hall J, Karplus P, Barbar E. Multivalency in the assembly of intrinsically disordered Dynein intermediate chain. J Biol Chem. 2009;284:33115-21 pubmed publisher
    b>Dynein light chains are thought to increase binding efficiency of dynein intermediate chain to both dynein heavy chain and dynactin, but their exact role is not clear...
  6. Hall J, Song Y, Karplus P, Barbar E. The crystal structure of dynein intermediate chain-light chain roadblock complex gives new insights into dynein assembly. J Biol Chem. 2010;285:22566-75 pubmed publisher
    The roadblock/LC7 dynein light chain is a ubiquitous component of all dyneins and is essential for many diverse processes including proper axonal transport and dendrite growth...
  7. Barbar E, Kleinman B, Imhoff D, Li M, Hays T, Hare M. Dimerization and folding of LC8, a highly conserved light chain of cytoplasmic dynein. Biochemistry. 2001;40:1596-605 pubmed
    b>Cytoplasmic dynein is a multisubunit ATPase that transforms chemical energy into motion along microtubules...
  8. Otani T, Oshima K, Onishi S, Takeda M, Shinmyozu K, Yonemura S, et al. IKK? regulates cell elongation through recycling endosome shuttling. Dev Cell. 2011;20:219-32 pubmed publisher
    ..IKK? regulates the localization of recycling endosome regulators Rab11 and Dynein and phosphorylates their adaptor molecule, Nuclear fallout (Nuf)...
  9. Otani T, Oshima K, Kimpara A, Takeda M, Abdu U, Hayashi S. A transport and retention mechanism for the sustained distal localization of Spn-F-IKKε during Drosophila bristle elongation. Development. 2015;142:2338-51 pubmed publisher
    ..Spindle-F (Spn-F), which is stably retained at the distal tip and functions as an adaptor linking IKKε to cytoplasmic dynein. We found that Javelin-like (Jvl) is a key regulator of Spn-F retention...
  10. Sengupta S, Rath U, Yao C, Zavortink M, Wang C, Girton J, et al. Digitor/dASCIZ Has Multiple Roles in Drosophila Development. PLoS ONE. 2016;11:e0166829 pubmed publisher
    ..We also confirm a previously documented function of Digitor/dASCIZ as a regulator of Dynein light chain/Cut up expression...
  11. Makokha M, Huang Y, Montelione G, Edison A, Barbar E. The solution structure of the pH-induced monomer of dynein light-chain LC8 from Drosophila. Protein Sci. 2004;13:727-34 pubmed
    The structure of Drosophila LC8 pH-induced monomer has been determined by NMR spectroscopy using the program AutoStructure...
  12. van Eyk C, O Keefe L, Lawlor K, Samaraweera S, McLeod C, Price G, et al. Perturbation of the Akt/Gsk3-? signalling pathway is common to Drosophila expressing expanded untranslated CAG, CUG and AUUCU repeat RNAs. Hum Mol Genet. 2011;20:2783-94 pubmed publisher
  13. Zaytseva O, Tenis N, Mitchell N, Kanno S, Yasui A, Heierhorst J, et al. The novel zinc finger protein dASCIZ regulates mitosis in Drosophila via an essential role in dynein light-chain expression. Genetics. 2014;196:443-53 pubmed publisher
    ..roles during lung organogenesis and B cell development in mice, where it regulates the expression of dynein light chain (DYNLL1/LC8), but its functions in other species including invertebrates are largely unknown...
  14. Song Y, Benison G, Nyarko A, Hays T, Barbar E. Potential role for phosphorylation in differential regulation of the assembly of dynein light chains. J Biol Chem. 2007;282:17272-9 pubmed
    The homodimeric light chains LC8 and Tctex-1 are integral parts of the microtubule motor cytoplasmic dynein, as they directly associate with dynein intermediate chain IC and various cellular cargoes...
  15. Chen K, Koe C, Xing Z, Tian X, Rossi F, Wang C, et al. Arl2- and Msps-dependent microtubule growth governs asymmetric division. J Cell Biol. 2016;212:661-76 pubmed publisher
    ..Moreover, Arl2 regulates dynein function and in turn centrosomal localization of D-TACC and Msps...
  16. Slevin L, Romes E, Dandulakis M, Slep K. The mechanism of dynein light chain LC8-mediated oligomerization of the Ana2 centriole duplication factor. J Biol Chem. 2014;289:20727-39 pubmed publisher
    ..Although recent work has demonstrated that an Ana2-dynein light chain (LC8) centriolar complex is critical for proper spindle positioning in neuroblasts, how Ana2 and LC8 ..
  17. Fischer J. Molecular motors and developmental asymmetry. Curr Opin Genet Dev. 2000;10:489-96 pubmed
    ..The extent of possible regulatory complexity is only beginning to emerge. ..
  18. Joti P, Ghosh Roy A, Ray K. Dynein light chain 1 functions in somatic cyst cells regulate spermatogonial divisions in Drosophila. Sci Rep. 2011;1:173 pubmed publisher
    ..The phenotype is dominantly enhanced by Dhc64C (cytoplasmic Dynein) and didum (Myosin V) loss-of-function alleles...
  19. Yang J, Bai J, Lee T. Dynein-dynactin complex is essential for dendritic restriction of TM1-containing Drosophila Dscam. PLoS ONE. 2008;3:e3504 pubmed publisher
    ..and by silencing specific genes via targeted RNAi, we found that several genes, encoding various components of the dynein-dynactin complex, are required for restricting Dscam[TM1] to the mushroom body dendrites...
  20. Dorus S, Freeman Z, Parker E, Heath B, Karr T. Recent origins of sperm genes in Drosophila. Mol Biol Evol. 2008;25:2157-66 pubmed publisher
    ..Thus, gene creation is a dynamic force in the evolution of sperm composition and possibly function, which further suggests that acquisition of molecular functionality in sperm may be an influential pathway in the fixation of new genes. ..
  21. Bullock S, Nicol A, Gross S, Zicha D. Guidance of bidirectional motor complexes by mRNA cargoes through control of dynein number and activity. Curr Biol. 2006;16:1447-52 pubmed
    ..We show that these proteins, together with the dynein motor, are recruited differentially to different RNA signals...
  22. Parassol N, Bienvenu C, Boglio C, Fiorucci S, Cerezo D, Yu X, et al. In vivo characterization of dynein-driven nanovectors using Drosophila oocytes. PLoS ONE. 2013;8:e82908 pubmed publisher
    ..We designed dynein motor driven FS using a collection of dynein light chain 8 (LC8) peptide binding motifs as molecular linkers and characterized in real time the efficiency of the FS ..
  23. BARRON D, MOBERG K. Inverse regulation of two classic Hippo pathway target genes in Drosophila by the dimerization hub protein Ctp. Sci Rep. 2016;6:22726 pubmed publisher
    ..Using the Drosophila wing as a developmental model, we find that the LC8 family member Cut up (Ctp) is primarily required to promote epithelial growth, which correlates with effects on the pro-growth factor ..
  24. Rom I, Faicevici A, Almog O, Neuman Silberberg F. Drosophila Dynein light chain (DDLC1) binds to gurken mRNA and is required for its localization. Biochim Biophys Acta. 2007;1773:1526-33 pubmed
    ..Here we report that the cytoplasmic Dynein-light-chain (DDLC1) which is the cargo-binding subunit of the Dynein motor protein, directly bound with high ..
  25. Benison G, Nyarko A, Barbar E. Heteronuclear NMR identifies a nascent helix in intrinsically disordered dynein intermediate chain: implications for folding and dimerization. J Mol Biol. 2006;362:1082-93 pubmed
    The intermediate chain of dynein forms a tight subcomplex with dimeric light chains LC8 and Tctex-1, and together they constitute the cargo attachment complex...
  26. Wang C, Li S, Januschke J, Rossi F, Izumi Y, Garcia Alvarez G, et al. An ana2/ctp/mud complex regulates spindle orientation in Drosophila neuroblasts. Dev Cell. 2011;21:520-33 pubmed publisher
    ..Here, we identified a protein complex composed of the tumor suppressor anastral spindle 2 (Ana2), a dynein light-chain protein Cut up (Ctp), and Mushroom body defect (Mud), which regulates mitotic spindle orientation...
  27. Kuznetsov A. Modelling active transport in Drosophila unipolar motor neurons. Comput Methods Biomech Biomed Engin. 2011;14:1117-31 pubmed publisher
    ..19:4122-4129) who proposed a map of microtubule (MT) orientation in Drosophila neurons, and explained why dynein mutations selectively impede dendritic growth without having much effect on axonal growth...
  28. Dimitriadi M, Sleigh J, Walker A, Chang H, Sen A, Kalloo G, et al. Conserved genes act as modifiers of invertebrate SMN loss of function defects. PLoS Genet. 2010;6:e1001172 pubmed publisher
  29. Weil T, Xanthakis D, Parton R, Dobbie I, Rabouille C, Gavis E, et al. Distinguishing direct from indirect roles for bicoid mRNA localization factors. Development. 2010;137:169-76 pubmed publisher
    ..immunoelectron microscopy on ultrathin frozen sections to study the distribution of Swallow, Staufen, actin and dynein relative to bicoid mRNA during late oogenesis...
  30. Nyarko A, Barbar E. Light chain-dependent self-association of dynein intermediate chain. J Biol Chem. 2011;286:1556-66 pubmed publisher
    b>Dynein light chains are bivalent dimers that bind two copies of dynein intermediate chain IC to form a cargo attachment subcomplex...
  31. Li X, Kuromi H, Briggs L, Green D, Rocha J, Sweeney S, et al. Bicaudal-D binds clathrin heavy chain to promote its transport and augments synaptic vesicle recycling. EMBO J. 2010;29:992-1006 pubmed publisher
    ..The Bicaudal-D (BicD) protein participates in the transport of a subset of cargoes by the minus-end-directed motor dynein, although the full extent of its functions is unclear...
  32. Nyarko A, Cochrun L, Norwood S, Pursifull N, Voth A, Barbar E. Ionization of His 55 at the dimer interface of dynein light-chain LC8 is coupled to dimer dissociation. Biochemistry. 2005;44:14248-55 pubmed
    LC8 is a highly conserved light-chain subunit of cytoplasmic dynein that interacts with a wide variety of cellular proteins and is presumed to play a fundamental role in dynein assembly and cargo recruitment and in the assembly of protein ..
  33. Abdu U, Bar D, Sch pbach T. spn-F encodes a novel protein that affects oocyte patterning and bristle morphology in Drosophila. Development. 2006;133:1477-84 pubmed publisher
    ..We also show that Spn-F interacts directly with the Dynein light chain Ddlc-1...
  34. Batlevi Y, Martin D, Pandey U, Simon C, Powers C, Taylor J, et al. Dynein light chain 1 is required for autophagy, protein clearance, and cell death in Drosophila. Proc Natl Acad Sci U S A. 2010;107:742-7 pubmed publisher
    ..that are required for salivary gland cell death, and here we describe the identification of Drosophila dynein light chain 1 (ddlc1) as a gene that is required for type II cell death...
  35. Murphey R, Caruccio P, Getzinger M, Westgate P, Phillis R. Dynein-dynactin function and sensory axon growth during Drosophila metamorphosis: A role for retrograde motors. Dev Biol. 1999;209:86-97 pubmed
    ..In Glued1 and Cytoplasmic dynein light chain mutants, proprioceptive and tactile axons arrive at the CNS on time but exhibit defects in ..
  36. Mohan P, Hosur R. NMR characterization of structural and dynamics perturbations due to a single point mutation in Drosophila DLC8 dimer: functional implications. Biochemistry. 2008;47:6251-9 pubmed publisher
    b>Dynein light chain protein (DLC8), the smallest subunit of the dynein motor complex, acts as a cargo adaptor. The protein exists as a dimer under physiological conditions, and cargo binding occurs at the dimer interface...
  37. Cohen R. Oocyte patterning: dynein and kinesin, inc. Curr Biol. 2002;12:R797-9 pubmed
    Recent studies show that dynein and kinesin are both required for cargo transport to the anterior cortex of the Drosophila oocyte...
  38. Stabell M, Bjørkmo M, Aalen R, Lambertsson A. The Drosophila SET domain encoding gene dEset is essential for proper development. Hereditas. 2006;143:177-88 pubmed
    ..Several interacting partners, for example USP, spire, and cut up were identified in a yeast two-hybrid screen...
  39. Stuchell Brereton M, Siglin A, Li J, Moore J, Ahmed S, Williams J, et al. Functional interaction between dynein light chain and intermediate chain is required for mitotic spindle positioning. Mol Biol Cell. 2011;22:2690-701 pubmed publisher
    b>Cytoplasmic dynein is a large multisubunit complex involved in retrograde transport and the positioning of various organelles...
  40. Asthana J, Kuchibhatla A, Jana S, Ray K, Panda D. Dynein light chain 1 (LC8) association enhances microtubule stability and promotes microtubule bundling. J Biol Chem. 2012;287:40793-805 pubmed publisher
    b>Dynein Light Chain 1 (LC8) has been shown to pull down tubulin subunits, suggesting that it interacts with microtubules. ..
  41. Winkler F, Gummalla M, Künneke L, Lv Z, Zippelius A, Aspelmeier T, et al. Fluctuation Analysis of Centrosomes Reveals a Cortical Function of Kinesin-1. Biophys J. 2015;109:856-68 pubmed publisher
    ..Kinesin-1 may mediate linkage of the microtubule (+)-ends to the actin cortex. Consistent with this model is our finding that Kinesin-1-GFP accumulates at the cortical actin caps. ..
  42. GONCZY P. Mechanisms of spindle positioning: focus on flies and worms. Trends Cell Biol. 2002;12:332-9 pubmed
    ..This article discusses recent findings that indicate how this mechanism might be used for spindle positioning during Drosophila and Caenorhabditis elegans development. ..
  43. Dick T, Bahri S, Chia W. Drosophila DPP2C1, a novel member of the protein phosphatase 2C (PP2C) family. Gene. 1997;199:139-43 pubmed
    ..The dpp2c1 gene maps to 4E1-2 on the X chromosome, 1.5 kb upstream of the ddlc1 gene...
  44. Kidane A, Song Y, Nyarko A, Hall J, Hare M, Lohr F, et al. Structural features of LC8-induced self-association of swallow. Biochemistry. 2013;52:6011-20 pubmed publisher
    ..b>Dynein light chain LC8, first discovered as a subunit of the motor protein dynein, is considered to have a role broader than that ..
  45. Shav Tal Y, Singer R. RNA localization. J Cell Sci. 2005;118:4077-81 pubmed
  46. Lin T, Pan P, Lai Y, Chiang K, Hsieh H, Wu Y, et al. Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons. PLoS Genet. 2015;11:e1005642 pubmed publisher
    ..of Spn-F by Ik2 kinase to decrease Spn-F self-association, and depends on the function of microtubule motor dynein complex...