crb

Summary

Gene Symbol: crb
Description: crumbs
Alias: 0509/20, 1384/04, CG6383, CRB, CT19912, Crb, Crbs, Crumbs, DmCrb, Dmel\CG6383, crumb, far, l(3)07207, l(3)S050920, l(3)S058104, l(3)j1B5, crumbs, CG6383-PA, CG6383-PB, CG6383-PC, CG6383-PD, crb-PA, crb-PB, crb-PC, crb-PD, fat rhabdomere, fat rhabdomeres, lethal (3) 07207
Species: fruit fly
Products:     crb

Top Publications

  1. Johnson K, Grawe F, Grzeschik N, Knust E. Drosophila crumbs is required to inhibit light-induced photoreceptor degeneration. Curr Biol. 2002;12:1675-80 pubmed
    ..The Drosophila homolog, crumbs, is required for polarity and adhesion in embryonic epithelia and for correct formation of adherens junctions and ..
  2. Pellikka M, Tanentzapf G, Pinto M, Smith C, McGlade C, Ready D, et al. Crumbs, the Drosophila homologue of human CRB1/RP12, is essential for photoreceptor morphogenesis. Nature. 2002;416:143-9 pubmed
    The apical transmembrane protein Crumbs is a central regulator of epithelial apical-basal polarity in Drosophila...
  3. Schottenfeld Roames J, Ghabrial A. Whacked and Rab35 polarize dynein-motor-complex-dependent seamless tube growth. Nat Cell Biol. 2012;14:386-93 pubmed publisher
    ..Thus, vesicle trafficking has key roles in making and shaping seamless tubes...
  4. Nam S, Choi K. Interaction of Par-6 and Crumbs complexes is essential for photoreceptor morphogenesis in Drosophila. Development. 2003;130:4363-72 pubmed
    ..b>Crumbs (Crb) is specifically localized to the apical membrane of photoreceptors, providing a positional cue for the ..
  5. Lemmers C, Médina E, Delgrossi M, Michel D, Arsanto J, Le Bivic A. hINADl/PATJ, a homolog of discs lost, interacts with crumbs and localizes to tight junctions in human epithelial cells. J Biol Chem. 2002;277:25408-15 pubmed
    ..We also identified a partial cDNA coding the transmembrane and cytoplasmic domains of a new human crumbs (CRB3) expressed in Caco-2 cells...
  6. Pocha S, Shevchenko A, Knust E. Crumbs regulates rhodopsin transport by interacting with and stabilizing myosin V. J Cell Biol. 2011;195:827-38 pubmed publisher
    The evolutionarily conserved Crumbs (Crb) complex is crucial for photoreceptor morphogenesis and homeostasis...
  7. Martinez A, Schuettengruber B, Sakr S, Janic A, Gonzalez C, Cavalli G. Polyhomeotic has a tumor suppressor activity mediated by repression of Notch signaling. Nat Genet. 2009;41:1076-82 pubmed publisher
    ..These data show that ph is a tumor suppressor locus that controls cellular proliferation by silencing multiple Notch signaling components. ..
  8. Bellaiche Y, Radovic A, Woods D, Hough C, Parmentier M, O Kane C, et al. The Partner of Inscuteable/Discs-large complex is required to establish planar polarity during asymmetric cell division in Drosophila. Cell. 2001;106:355-66 pubmed
    ..Finally, Baz and the Dlg/Pins complex are required for the asymmetric localization of Numb. Thus, the Dlg/Pins complex responds to Fz signaling to establish planar asymmetry in the pI cell. ..
  9. Seshaiah P, Miller B, Myat M, Andrew D. pasilla, the Drosophila homologue of the human Nova-1 and Nova-2 proteins, is required for normal secretion in the salivary gland. Dev Biol. 2001;239:309-22 pubmed
    ..The secretory defects in ps mutants provide a potential mechanism for the loss of motor function observed in POMA patients. ..

More Information

Publications99

  1. Banerjee S, Bainton R, Mayer N, Beckstead R, Bhat M. Septate junctions are required for ommatidial integrity and blood-eye barrier function in Drosophila. Dev Biol. 2008;317:585-99 pubmed publisher
    ..Our studies may provide clues towards understanding the vertebrate BEB formation and function. ..
  2. Fuss B, Hoch M. Notch signaling controls cell fate specification along the dorsoventral axis of the Drosophila gut. Curr Biol. 2002;12:171-9 pubmed
    ..which encode nuclear steroid receptors, are Notch target genes required for the expression of crumbs, which encodes a transmembrane protein involved in organizing apical-basal polarity...
  3. Tepass U. Crumbs, a component of the apical membrane, is required for zonula adherens formation in primary epithelia of Drosophila. Dev Biol. 1996;177:217-25 pubmed
    ..A second specific marker for primary epithelia is the Crumbs protein, which in concert with the gene product of stardust is required to maintain epithelial polarity...
  4. Lu H, Bilder D. Endocytic control of epithelial polarity and proliferation in Drosophila. Nat Cell Biol. 2005;7:1232-9 pubmed
    ..accumulation of specific membrane proteins, including the Notch signalling receptor and the polarity determinant Crumbs (Crb)...
  5. Okajima T, Xu A, Lei L, Irvine K. Chaperone activity of protein O-fucosyltransferase 1 promotes notch receptor folding. Science. 2005;307:1599-603 pubmed
    ..This ability of OFUT1 to facilitate folding of Notch did not require its fucosyltransferase activity. Thus, a glycosyltransferase can bind its substrate in the endoplasmic reticulum to facilitate normal folding. ..
  6. Muschalik N, Knust E. Increased levels of the cytoplasmic domain of Crumbs repolarise developing Drosophila photoreceptors. J Cell Sci. 2011;124:3715-25 pubmed publisher
    ..The evolutionarily conserved transmembrane protein Crumbs (Crb) organises an apical membrane-associated protein complex that controls photoreceptor morphogenesis...
  7. Horne Badovinac S, Bilder D. Dynein regulates epithelial polarity and the apical localization of stardust A mRNA. PLoS Genet. 2008;4:e8 pubmed publisher
    ..that Dynein acts through the cytoplasmic scaffolding protein Stardust (Sdt) to localize the transmembrane protein Crumbs, in part through the apical targeting of specific sdt mRNA isoforms...
  8. League G, Nam S. Role of kinesin heavy chain in Crumbs localization along the rhabdomere elongation in Drosophila photoreceptor. PLoS ONE. 2011;6:e21218 pubmed publisher
    b>Crumbs (Crb), a cell polarity gene, has been shown to provide a positional cue for the extension of the apical membrane domain, adherens junction (AJ), and rhabdomere along the growing proximal-distal axis during Drosophila photoreceptor ..
  9. Médina E, Lemmers C, Lane Guermonprez L, Le Bivic A. Role of the Crumbs complex in the regulation of junction formation in Drosophila and mammalian epithelial cells. Biol Cell. 2002;94:305-13 pubmed
    ..We know now that Crumbs, the product of crb is an apical transmembrane protein conserved in mammals and that it interacts by its ..
  10. Husain N, Pellikka M, Hong H, Klimentova T, Choe K, Clandinin T, et al. The agrin/perlecan-related protein eyes shut is essential for epithelial lumen formation in the Drosophila retina. Dev Cell. 2006;11:483-93 pubmed
    ..Our findings show that the secretion of a proteoglycan into the apical matrix is critical for the formation of epithelial lumina in the fly retina. ..
  11. Harris K, Tepass U. Cdc42 and Par proteins stabilize dynamic adherens junctions in the Drosophila neuroectoderm through regulation of apical endocytosis. J Cell Biol. 2008;183:1129-43 pubmed publisher
    ..caused an increase in the endocytotic uptake of apical proteins, including apical polarity factors such as Crumbs, which are required for AJ stability...
  12. Tepass U. Epithelial differentiation in Drosophila. Bioessays. 1997;19:673-82 pubmed
    ..Two key regulators of epithelial polarity, Crumbs and DE-cadherin, have been studied at the genetic and molecular levels and a number of additional genes are being ..
  13. Isaac D, Andrew D. Tubulogenesis in Drosophila: a requirement for the trachealess gene product. Genes Dev. 1996;10:103-17 pubmed
    ..trh is homologous to two transcription factors: the human hypoxia-inducible factor-1 alpha and the Drosophila Single-minded protein. ..
  14. Maybeck V, Röper K. A targeted gain-of-function screen identifies genes affecting salivary gland morphogenesis/tubulogenesis in Drosophila. Genetics. 2009;181:543-65 pubmed publisher
    ..We report the initial characterization of a subset of genes, including chickadee, rhomboid1, egalitarian, bitesize, and capricious, through comparison of gain- and loss-of-function phenotypes. ..
  15. Gibson M, Perrimon N. Apicobasal polarization: epithelial form and function. Curr Opin Cell Biol. 2003;15:747-52 pubmed
    ..Recent findings show that segregation of ligand and receptor with one on each side of this barrier can be a crucial regulator of cell-cell signaling events. ..
  16. Fletcher G, Lucas E, Brain R, Tournier A, Thompson B. Positive feedback and mutual antagonism combine to polarize Crumbs in the Drosophila follicle cell epithelium. Curr Biol. 2012;22:1116-22 pubmed publisher
    ..In the Drosophila ovarian follicle cell epithelium, apical membranes are specified by Crumbs (Crb), Stardust (Sdt), and the aPKC-Par6-cdc42 complex...
  17. Morais de Sá E, Mirouse V, St Johnston D. aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells. Cell. 2010;141:509-23 pubmed publisher
    ..Removal of Baz from the complex is epithelial-specific because it also requires the Crumbs complex, which prevents the Baz/PAR-6 interaction...
  18. Szafranski P, Goode S. A Fasciclin 2 morphogenetic switch organizes epithelial cell cluster polarity and motility. Development. 2004;131:2023-36 pubmed
    ..Furthermore, our results provide novel insight into the intimate link between epithelial polarity and acquisition of motile polarity that has important implications for development of invasive carcinomas. ..
  19. Iwaki D, Lengyel J. A Delta-Notch signaling border regulated by Engrailed/Invected repression specifies boundary cells in the Drosophila hindgut. Mech Dev. 2002;114:71-84 pubmed
    ..Boundary cells are distinguished by their elongated morphology, high level of both apical and cytoplasmic Crb protein, and gene expression program...
  20. Lamb R, Ward R, Schweizer L, Fehon R. Drosophila coracle, a member of the protein 4.1 superfamily, has essential structural functions in the septate junctions and developmental functions in embryonic and adult epithelial cells. Mol Biol Cell. 1998;9:3505-19 pubmed
    ..1 superfamily members. These studies provide insights into a range of in vivo functions for coracle in developing embryos and adults. ..
  21. Dumstrei K, Wang F, Shy D, Tepass U, Hartenstein V. Interaction between EGFR signaling and DE-cadherin during nervous system morphogenesis. Development. 2002;129:3983-94 pubmed
    ..Finally, EGFR can be co-immunoprecipitated with anti-DE-cadherin and anti-Armadillo antibodies from embryonic protein extracts. We propose that EGFR signaling plays a role in morphogenesis by modulating cell adhesion. ..
  22. Iwaki D, Johansen K, Singer J, Lengyel J. drumstick, bowl, and lines are required for patterning and cell rearrangement in the Drosophila embryonic hindgut. Dev Biol. 2001;240:611-26 pubmed
    ..The close association of both cell rearrangement and patterning defects in all three mutants suggest that proper patterning of the hindgut into small intestine and large intestine is likely required for its correct morphogenesis. ..
  23. Bachmann A, Schneider M, Theilenberg E, Grawe F, Knust E. Drosophila Stardust is a partner of Crumbs in the control of epithelial cell polarity. Nature. 2001;414:638-43 pubmed
    ..A core component of the SAC is the transmembrane protein Crumbs, the cytoplasmic domain of which recruits the PDZ-protein Discs Lost into the complex...
  24. Li J, Li W, Calhoun H, Xia F, Gao F, Li W. Patterns and functions of STAT activation during Drosophila embryogenesis. Mech Dev. 2003;120:1455-68 pubmed
    ..These results suggest that STAT activation is involved in proper differentiation and morphogenesis of multiple tissues during Drosophila embryogenesis. ..
  25. White K, Grether M, Abrams J, Young L, Farrell K, Steller H. Genetic control of programmed cell death in Drosophila. Science. 1994;264:677-83 pubmed
    ..The reaper gene appears to encode a small peptide that shows no homology to known proteins, and reaper messenger RNA is expressed in cells destined to undergo apoptosis. ..
  26. Kerman B, Cheshire A, Myat M, Andrew D. Ribbon modulates apical membrane during tube elongation through Crumbs and Moesin. Dev Biol. 2008;320:278-88 pubmed publisher
    ..Lola Like, another BTB-domain containing protein required for robust nuclear localization of Ribbon, to upregulate crumbs expression and downregulate Moesin activity...
  27. Robinson B, Huang J, Hong Y, Moberg K. Crumbs regulates Salvador/Warts/Hippo signaling in Drosophila via the FERM-domain protein Expanded. Curr Biol. 2010;20:582-90 pubmed publisher
    ..We have tested the basis of an overgrowth phenotype driven by the Drosophila protein Crumbs (Crb), which nucleates an apical membrane complex that functionally interacts with the Par6/Par3/aPKC and Scrib/..
  28. Szafranski P, Goode S. Basolateral junctions are sufficient to suppress epithelial invasion during Drosophila oogenesis. Dev Dyn. 2007;236:364-73 pubmed
    ..Our results demonstrate that spatiotemporal patterns of basolateral junction activity directly suppress epithelial invasion by organizing the cooperative activity of distinct polarity and motility pathways. ..
  29. den Hollander A, Ghiani M, de Kok Y, Wijnholds J, Ballabio A, Cremers F, et al. Isolation of Crb1, a mouse homologue of Drosophila crumbs, and analysis of its expression pattern in eye and brain. Mech Dev. 2002;110:203-7 pubmed
    Mutations in the human Crumbs homologue 1 (CRB1) gene cause severe retinal dystrophies. CRB1 is homologous to Drosophila Crumbs, a protein essential for establishing and maintaining epithelial polarity...
  30. Chartier F, Hardy E, Laprise P. Crumbs controls epithelial integrity by inhibiting Rac1 and PI3K. J Cell Sci. 2011;124:3393-8 pubmed publisher
    Drosophila Crumbs (Crb) and its mammalian ortholog CRB3 control epithelial polarity through poorly understood molecular mechanisms...
  31. Hong Y, Stronach B, Perrimon N, Jan L, Jan Y. Drosophila Stardust interacts with Crumbs to control polarity of epithelia but not neuroblasts. Nature. 2001;414:634-8 pubmed
    ..Initially discovered in the landmark genetic screen for Drosophila developmental mutants, bazooka, crumbs, shotgun and stardust mutants exhibit severe disruption in apicobasal polarity in embryonic epithelia, resulting ..
  32. Bilder D, Schober M, Perrimon N. Integrated activity of PDZ protein complexes regulates epithelial polarity. Nat Cell Biol. 2003;5:53-8 pubmed
    ..The Sdt-containing Crb complex is recruited apically by the Baz complex to counter antagonistic Scrib activity...
  33. Widmann T, Dahmann C. Dpp signaling promotes the cuboidal-to-columnar shape transition of Drosophila wing disc epithelia by regulating Rho1. J Cell Sci. 2009;122:1362-73 pubmed publisher
    ..Our results identify a cell-autonomous role for Dpp signaling in promoting and maintaining the elongated columnar shape of wing disc cells and suggest that Dpp signaling acts by regulating Rho1 and MRLC. ..
  34. Morrison H, Dionne H, Rusten T, Brech A, Fisher W, Pfeiffer B, et al. Regulation of early endosomal entry by the Drosophila tumor suppressors Rabenosyn and Vps45. Mol Biol Cell. 2008;19:4167-76 pubmed publisher
    ..This work represents the first characterization of a Rab5 effector as a tumor suppressor, and it provides in vivo evidence for a Rbsn-Vps45 complex on early endosomes that links Rab5 to the SNARE fusion machinery. ..
  35. Tonning A, Helms S, Schwarz H, Uv A, Moussian B. Hormonal regulation of mummy is needed for apical extracellular matrix formation and epithelial morphogenesis in Drosophila. Development. 2006;133:331-41 pubmed
  36. Dong B, Hannezo E, Hayashi S. Balance between apical membrane growth and luminal matrix resistance determines epithelial tubule shape. Cell Rep. 2014;7:941-50 pubmed publisher
    ..is caused by excessive apical membrane synthesis accompanied by the ectopic accumulation and overactivation of Crumbs in swollen endosomes...
  37. David D, Tishkina A, Harris T. The PAR complex regulates pulsed actomyosin contractions during amnioserosa apical constriction in Drosophila. Development. 2010;137:1645-55 pubmed publisher
    ..These results identify the mechanics of actomyosin contractility that drive amnioserosa apical constriction and how specific steps of the contractile mechanism are regulated by the PAR complex...
  38. Pocha S, Wassmer T, Niehage C, Hoflack B, Knust E. Retromer controls epithelial cell polarity by trafficking the apical determinant Crumbs. Curr Biol. 2011;21:1111-7 pubmed publisher
    The evolutionarily conserved apical determinant Crumbs (Crb) is essential for maintaining apicobasal polarity and integrity of many epithelial tissues [1]...
  39. Brodsky M, Nordstrom W, Tsang G, Kwan E, Rubin G, Abrams J. Drosophila p53 binds a damage response element at the reaper locus. Cell. 2000;101:103-13 pubmed
    ..These results indicate that reaper is a direct transcriptional target of Drosophila p53 following DNA damage. ..
  40. Kolahgar G, Bardet P, Langton P, Alexandre C, Vincent J. Apical deficiency triggers JNK-dependent apoptosis in the embryonic epidermis of Drosophila. Development. 2011;138:3021-31 pubmed publisher
    ..Transcriptional profiling and in situ hybridisation show that JNK signalling is upregulated in mutants lacking Crumbs or other apical determinants...
  41. Röper K. Anisotropy of Crumbs and aPKC drives myosin cable assembly during tube formation. Dev Cell. 2012;23:939-53 pubmed publisher
    ..of the Drosophila salivary glands, I find that an anisotropic plasma membrane distribution of the protein Crumbs, mediated by its large extracellular domain, determines the subcellular localization of a supracellular actomyosin ..
  42. Behr M, Riedel D, Schuh R. The claudin-like megatrachea is essential in septate junctions for the epithelial barrier function in Drosophila. Dev Cell. 2003;5:611-20 pubmed
    ..The results indicate that claudin-like proteins are functionally conserved between vertebrates and Drosophila. ..
  43. Chen C, Gajewski K, Hamaratoglu F, Bossuyt W, Sansores Garcia L, Tao C, et al. The apical-basal cell polarity determinant Crumbs regulates Hippo signaling in Drosophila. Proc Natl Acad Sci U S A. 2010;107:15810-5 pubmed publisher
    ..Here, we investigated the mechanism by which the apical determinant Crumbs (Crb) affects growth in Drosophila imaginal discs...
  44. Mitonaka T, Muramatsu Y, Sugiyama S, Mizuno T, Nishida Y. Essential roles of myosin phosphatase in the maintenance of epithelial cell integrity of Drosophila imaginal disc cells. Dev Biol. 2007;309:78-86 pubmed
    ..The requirement of DMBS for the integrity of static epithelial cells in imaginal discs suggests that the regulation of Myosin II by DMBS has a role more general than its previously demonstrated functions in morphogenetic events. ..
  45. Li B, Satoh A, Ready D. Myosin V, Rab11, and dRip11 direct apical secretion and cellular morphogenesis in developing Drosophila photoreceptors. J Cell Biol. 2007;177:659-69 pubmed
    ..A protein trio conserved across eukaryotes thus mediates normal, in vivo sensory neuron morphogenesis. ..
  46. Moberg K, Schelble S, Burdick S, Hariharan I. Mutations in erupted, the Drosophila ortholog of mammalian tumor susceptibility gene 101, elicit non-cell-autonomous overgrowth. Dev Cell. 2005;9:699-710 pubmed
    ..Activation of JAK-STAT signaling in surrounding wild-type cells correlates with their overgrowth. These findings define a pathway by which changes in endocytic trafficking can regulate tissue growth in a non-cell-autonomous manner. ..
  47. Wodarz A, Grawe F, Knust E. CRUMBS is involved in the control of apical protein targeting during Drosophila epithelial development. Mech Dev. 1993;44:175-87 pubmed
    The gene crumbs (crb) of Drosophila encodes a transmembrane protein with 30 EGF-like and four laminin A G-domain-like repeats in its extracellular domain...
  48. Leptin M. Gastrulation in Drosophila: the logic and the cellular mechanisms. EMBO J. 1999;18:3187-92 pubmed
  49. Beronja S, Laprise P, Papoulas O, Pellikka M, Sisson J, Tepass U. Essential function of Drosophila Sec6 in apical exocytosis of epithelial photoreceptor cells. J Cell Biol. 2005;169:635-46 pubmed
    ..Furthermore, we show that Rab11 forms a complex with Sec5 and that Sec5 interacts with Sec6 suggesting that the exocyst is a Rab11 effector that facilitates protein transport to the apical rhabdomere in Drosophila PRCs. ..
  50. Tanentzapf G, Tepass U. Interactions between the crumbs, lethal giant larvae and bazooka pathways in epithelial polarization. Nat Cell Biol. 2003;5:46-52 pubmed
    ..b>Crumbs (Crb) and Lethal giant larvae (Lgl) are components of distinct complexes that regulate epithelial polarization in ..
  51. Wang S, Jayaram S, Hemphälä J, Senti K, Tsarouhas V, Jin H, et al. Septate-junction-dependent luminal deposition of chitin deacetylases restricts tube elongation in the Drosophila trachea. Curr Biol. 2006;16:180-5 pubmed
    ..However, the underlying molecular mechanisms controlling tube expansion so far remained elusive...
  52. Moussian B, Tång E, Tonning A, Helms S, Schwarz H, NUSSLEIN VOLHARD C, et al. Drosophila Knickkopf and Retroactive are needed for epithelial tube growth and cuticle differentiation through their specific requirement for chitin filament organization. Development. 2006;133:163-71 pubmed
    ..We propose a model in which Knk and the predicted chitin-binding protein Rtv form membrane complexes essential for epithelial tubulogenesis and cuticle formation through their specific role in directing chitin filament assembly...
  53. Margolis B, Borg J. Apicobasal polarity complexes. J Cell Sci. 2005;118:5157-9 pubmed
  54. Tepass U, Theres C, Knust E. crumbs encodes an EGF-like protein expressed on apical membranes of Drosophila epithelial cells and required for organization of epithelia. Cell. 1990;61:787-99 pubmed
    We describe the molecular characterization of the Drosophila gene crumbs, which encodes an integral membrane protein with 30 EGF-like repeats in the extracellular part and exhibits a striking expression pattern...
  55. Dunlop J, Morin X, Corominas M, Serras F, Tear G. glaikit is essential for the formation of epithelial polarity and neuronal development. Curr Biol. 2004;14:2039-45 pubmed
    ..The Crumbs (Crb) complex is one of three separate complexes that cooperate to control epithelial polarity and the formation ..
  56. Wu V, Beitel G. A junctional problem of apical proportions: epithelial tube-size control by septate junctions in the Drosophila tracheal system. Curr Opin Cell Biol. 2004;16:493-9 pubmed
    ..Possible tube-size functions of septate junctions include patterning of the apical extracellular matrix and regulation of conserved cell polarity genes such as Scribble and Discs Large. ..
  57. Leong G, Goulding K, Amin N, Richardson H, Brumby A. Scribble mutants promote aPKC and JNK-dependent epithelial neoplasia independently of Crumbs. BMC Biol. 2009;7:62 pubmed publisher
    ..First, we show that over-expression of the apical cell polarity determinants Crumbs (Crb) or aPKC induces similar cell morphology defects and over-proliferation phenotypes as scrib loss-of-function...
  58. Bulgakova N, Kempkens O, Knust E. Multiple domains of Stardust differentially mediate localisation of the Crumbs-Stardust complex during photoreceptor development in Drosophila. J Cell Sci. 2008;121:2018-26 pubmed publisher
    ..Sdt), a member of the MAGUK family of scaffolding proteins, is a constituent of the evolutionarily conserved Crumbs-Stardust (Crb-Sdt) complex that controls epithelial cell polarity in the embryo and morphogenesis of photoreceptor ..
  59. Karagiosis S, Ready D. Moesin contributes an essential structural role in Drosophila photoreceptor morphogenesis. Development. 2004;131:725-32 pubmed
    ..Our results show an essential structural role for Moesin in photoreceptor morphology. ..
  60. Tanentzapf G, Smith C, McGlade J, Tepass U. Apical, lateral, and basal polarization cues contribute to the development of the follicular epithelium during Drosophila oogenesis. J Cell Biol. 2000;151:891-904 pubmed
    ..Moreover, we use mosaic analysis to show that Crumbs (Crb) is required for the formation and maintenance of the follicular epithelium...
  61. Knust E. Control of epithelial cell shape and polarity. Curr Opin Genet Dev. 2000;10:471-5 pubmed
    ..Recent data have provided new insights into the molecular nature of these cues and the mechanisms by which they may be translated into a polarised phenotype. ..
  62. Franz A, Riechmann V. Stepwise polarisation of the Drosophila follicular epithelium. Dev Biol. 2010;338:136-47 pubmed publisher
    ..Apical membrane specification continues by the accumulation of the Crumbs complex, which is controlled by Par-3, Par-6, and aPKC...
  63. Bilder D, Li M, Perrimon N. Cooperative regulation of cell polarity and growth by Drosophila tumor suppressors. Science. 2000;289:113-6 pubmed
    ..scrib, dlg, and lgl show strong genetic interactions. Our data indicate that the three tumor suppressors act together in a common pathway to regulate cell polarity and growth control. ..
  64. Sansores Garcia L, Bossuyt W, Wada K, Yonemura S, Tao C, Sasaki H, et al. Modulating F-actin organization induces organ growth by affecting the Hippo pathway. EMBO J. 2011;30:2325-35 pubmed publisher
    ..Thus, regulators of F-actin, and in particular Capping proteins, are essential for proper growth control by affecting Hippo signalling. ..
  65. Cox R, Kirkpatrick C, Peifer M. Armadillo is required for adherens junction assembly, cell polarity, and morphogenesis during Drosophila embryogenesis. J Cell Biol. 1996;134:133-48 pubmed
    ..These results provide the first demonstration of the effect of loss of adherens junctions on Drosophila embryonic development. ..
  66. Huang J, Zhou W, Watson A, Jan Y, Hong Y. Efficient ends-out gene targeting in Drosophila. Genetics. 2008;180:703-7 pubmed publisher
    ..We generated new sets of targeting vectors and fly stocks and introduced a novel negative selection marker that drastically reduced the frequency of false-positive targeting candidates. ..
  67. Peng C, Manning L, Albertson R, Doe C. The tumour-suppressor genes lgl and dlg regulate basal protein targeting in Drosophila neuroblasts. Nature. 2000;408:596-600 pubmed
    ..We conclude that Dlg and Lgl promote, and myosin II inhibits, actomyosin-dependent basal protein targeting in neuroblasts. ..
  68. Médina E, Williams J, Klipfell E, Zarnescu D, Thomas G, Le Bivic A. Crumbs interacts with moesin and beta(Heavy)-spectrin in the apical membrane skeleton of Drosophila. J Cell Biol. 2002;158:941-51 pubmed
    The apical transmembrane protein Crumbs is necessary for both cell polarization and the assembly of the zonula adherens (ZA) in Drosophila epithelia...
  69. Herranz H, Stamataki E, Feiguin F, Milan M. Self-refinement of Notch activity through the transmembrane protein Crumbs: modulation of gamma-secretase activity. EMBO Rep. 2006;7:297-302 pubmed
    ..Here, we show that the transmembrane protein Crumbs is involved in a feedback mechanism used by Notch to refine its own activation domain at the Drosophila wing ..
  70. Li Z, Wang L, Hays T, Cai Y. Dynein-mediated apical localization of crumbs transcripts is required for Crumbs activity in epithelial polarity. J Cell Biol. 2008;180:31-8 pubmed publisher
    ..The conserved transmembrane protein Crumbs (Crb) is an important regulator of epithelial polarity...
  71. Tsarouhas V, Senti K, Jayaram S, Tiklová K, Hemphälä J, Adler J, et al. Sequential pulses of apical epithelial secretion and endocytosis drive airway maturation in Drosophila. Dev Cell. 2007;13:214-25 pubmed
    ..The coordination of luminal matrix secretion and endocytosis may be a general mechanism in tubular organ morphogenesis and maturation. ..
  72. Grzeschik N, Parsons L, Allott M, Harvey K, Richardson H. Lgl, aPKC, and Crumbs regulate the Salvador/Warts/Hippo pathway through two distinct mechanisms. Curr Biol. 2010;20:573-81 pubmed publisher
    ..Ectopic expression of the apical polarity regulators atypical protein kinase C (aPKC) and Crumbs also leads to increased cell proliferation and/or survival...
  73. Campbell K, Knust E, Skaer H. Crumbs stabilises epithelial polarity during tissue remodelling. J Cell Sci. 2009;122:2604-12 pubmed publisher
    ..Scribble protein groups are required for the establishment of tubule cell polarity, the key apical determinant, Crumbs, is required for cell polarity in the tubules only from the time when morphogenetic movements start...
  74. Pielage J, Stork T, Bunse I, Kl mbt C. The Drosophila cell survival gene discs lost encodes a cytoplasmic Codanin-1-like protein, not a homolog of tight junction PDZ protein Patj. Dev Cell. 2003;5:841-51 pubmed
    ..Finally, we present evidence that the PDZ protein previously thought to be encoded by dlt is not required for viability. We propose to rename this PDZ protein after its vertebrate homolog, Patj (Pals-associated tight junction protein)...
  75. Bayraktar J, Zygmunt D, Carthew R. Par-1 kinase establishes cell polarity and functions in Notch signaling in the Drosophila embryo. J Cell Sci. 2006;119:711-21 pubmed
    ..Epistasis analysis indicates that Par-1 functions upstream of Notch and is critical for proper localization of the Notch ligand Delta. ..
  76. Laprise P, Beronja S, Silva Gagliardi N, Pellikka M, Jensen A, McGlade C, et al. The FERM protein Yurt is a negative regulatory component of the Crumbs complex that controls epithelial polarity and apical membrane size. Dev Cell. 2006;11:363-74 pubmed
    The Crumbs (Crb) complex is a key regulator of epithelial cell architecture where it promotes apical membrane formation...
  77. BACHMANN A, Draga M, Grawe F, Knust E. On the role of the MAGUK proteins encoded by Drosophila varicose during embryonic and postembryonic development. BMC Dev Biol. 2008;8:55 pubmed publisher
  78. Takashima S, Murakami R. Regulation of pattern formation in the Drosophila hindgut by wg, hh, dpp, and en. Mech Dev. 2001;101:79-90 pubmed
    ..We propose a model for the gene regulatory pathways leading to the subdivision of the hindgut into domains. ..
  79. Massarwa R, Schejter E, Shilo B. Apical secretion in epithelial tubes of the Drosophila embryo is directed by the Formin-family protein Diaphanous. Dev Cell. 2009;16:877-88 pubmed publisher
    ..This mechanism allows efficient utilization of the entire apical membrane for secretion. ..
  80. Johnson K, Wodarz A. A genetic hierarchy controlling cell polarity. Nat Cell Biol. 2003;5:12-4 pubmed
  81. Myat M, Andrew D. Epithelial tube morphology is determined by the polarized growth and delivery of apical membrane. Cell. 2002;111:879-91 pubmed
    ..cell shape change and apical membrane growth during salivary cell invagination via two downstream target genes, crumbs (crb), a determinant of the apical membrane, and klarsicht (klar), which mediates microtubule-dependent organelle ..
  82. Knust E, Bossinger O. Composition and formation of intercellular junctions in epithelial cells. Science. 2002;298:1955-9 pubmed
    ..Comparisons between fly, worm, and vertebrate epithelia reveal marked similarities with respect to the molecules used, and pronounced differences in the organization of the junctions themselves. ..
  83. Tanaka Matakatsu M, Uemura T, Oda H, Takeichi M, Hayashi S. Cadherin-mediated cell adhesion and cell motility in Drosophila trachea regulated by the transcription factor Escargot. Development. 1996;122:3697-705 pubmed
  84. Zhou W, Hong Y. Drosophila Patj plays a supporting role in apical-basal polarity but is essential for viability. Development. 2012;139:2891-6 pubmed publisher
    ..Studies of Drosophila and mammalian cells suggest that Patj is required for the apical polarity protein complex Crumbs-Stardust (Pals1 or Mpp5 in mammalian cells) to establish apical-basal polarity...
  85. Green R, Hatini V, Johansen K, Liu X, Lengyel J. Drumstick is a zinc finger protein that antagonizes Lines to control patterning and morphogenesis of the Drosophila hindgut. Development. 2002;129:3645-56 pubmed
    ..We have thus defined a pathway in which a spatially localized zinc finger protein antagonizes a globally expressed protein, thereby leading to specification of a domain (the small intestine) necessary for oriented cell rearrangement. ..
  86. Xu N, Keung B, Myat M. Rho GTPase controls invagination and cohesive migration of the Drosophila salivary gland through Crumbs and Rho-kinase. Dev Biol. 2008;321:88-100 pubmed publisher
    ..We demonstrate that the absence of zygotic function of Rho1 results in the selective loss of the apical proteins, Crumbs (Crb), Drosophila atypical PKC and Stardust during gland invagination and that this is partially due to reduced ..
  87. Bilder D, Perrimon N. Localization of apical epithelial determinants by the basolateral PDZ protein Scribble. Nature. 2000;403:676-80 pubmed
    ..These phenotypes can be accounted for by mislocalization of the apical determinant Crumbs. Our results show that the lateral domain of epithelia, particularly the septate junction, functions in ..
  88. Walther R, Pichaud F. Crumbs/DaPKC-dependent apical exclusion of Bazooka promotes photoreceptor polarity remodeling. Curr Biol. 2010;20:1065-74 pubmed publisher
    ..This depends on the intertwined function of Bazooka (Baz), Par6-DaPKC, and the Crumbs (Crb)-Stardust (Sdt)-PATJ complex...
  89. Seshaiah P, Andrew D. WRS-85D: A tryptophanyl-tRNA synthetase expressed to high levels in the developing Drosophila salivary gland. Mol Biol Cell. 1999;10:1595-608 pubmed
    ..We also discuss the potential noncanonical activities of tryptophanyl-tRNA synthetase in immune response and regulation of cell growth. ..
  90. Genevet A, Polesello C, Blight K, Robertson F, Collinson L, Pichaud F, et al. The Hippo pathway regulates apical-domain size independently of its growth-control function. J Cell Sci. 2009;122:2360-70 pubmed publisher
    ..wing imaginal disc epithelium, we observe increased staining for members of the apical polarity complexes aPKC and Crumbs as well as adherens junction components when Hippo activity is compromised, while basolateral markers are not ..