Gene Symbol: complexin
Description: complexin
Alias: BcDNA:GH27718, BcDNA:HL04936, BcDNA:RH26459, BcDNA:RH31085, CG32490, CPX, Cpx, DmCpx, Dmel\CG32490, dmCplx, complexin, CG32490-PA, CG32490-PAA, CG32490-PAB, CG32490-PC, CG32490-PE, CG32490-PG, CG32490-PH, CG32490-PI, CG32490-PJ, CG32490-PK, CG32490-PL, CG32490-PM, CG32490-PO, CG32490-PP, CG32490-PS, CG32490-PU, CG32490-PV, CG32490-PW, CG32490-PX, CG32490-PY, CG32490-PZ, cpx-PA, cpx-PAA, cpx-PAB, cpx-PC, cpx-PE, cpx-PG, cpx-PH, cpx-PI, cpx-PJ, cpx-PK, cpx-PL, cpx-PM, cpx-PO, cpx-PP, cpx-PS, cpx-PU, cpx-PV, cpx-PW, cpx-PX, cpx-PY, cpx-PZ
Species: fruit fly

Top Publications

  1. Huntwork S, Littleton J. A complexin fusion clamp regulates spontaneous neurotransmitter release and synaptic growth. Nat Neurosci. 2007;10:1235-7 pubmed
    ..Here we demonstrate that spontaneous release at synapses is regulated by complexin, a SNARE complex-binding protein...
  2. Iyer J, Wahlmark C, Kuser Ahnert G, Kawasaki F. Molecular mechanisms of COMPLEXIN fusion clamp function in synaptic exocytosis revealed in a new Drosophila mutant. Mol Cell Neurosci. 2013;56:244-54 pubmed publisher
    The COMPLEXIN (CPX) proteins play a critical role in synaptic vesicle fusion and neurotransmitter release...
  3. Jorquera R, Huntwork Rodriguez S, Akbergenova Y, Cho R, Littleton J. Complexin controls spontaneous and evoked neurotransmitter release by regulating the timing and properties of synaptotagmin activity. J Neurosci. 2012;32:18234-45 pubmed publisher
    ..of SNARE-binding proteins have evolved to regulate synaptic exocytosis, including Synaptotagmin (SYT) and Complexin (CPX)...
  4. Mahoney R, Azpurua J, Eaton B. Insulin signaling controls neurotransmission via the 4eBP-dependent modification of the exocytotic machinery. elife. 2016;5: pubmed publisher
    ..A critical target of this regulatory mechanism is Complexin, a synaptic protein known to regulate synaptic vesicle exocytosis...
  5. Hoopengardner B, Bhalla T, Staber C, Reenan R. Nervous system targets of RNA editing identified by comparative genomics. Science. 2003;301:832-6 pubmed
    ..These results point to a pivotal role for RNA editing in nervous system function. ..
  6. Cho R, Buhl L, Volfson D, Tran A, Li F, Akbergenova Y, et al. Phosphorylation of Complexin by PKA Regulates Activity-Dependent Spontaneous Neurotransmitter Release and Structural Synaptic Plasticity. Neuron. 2015;88:749-61 pubmed publisher
    ..Here we show that the SNARE fusion clamp Complexin undergoes activity-dependent phosphorylation that alters the basic properties of neurotransmission in Drosophila...
  7. Bykhovskaia M, Jagota A, González A, Vasin A, Littleton J. Interaction of the complexin accessory helix with the C-terminus of the SNARE complex: molecular-dynamics model of the fusion clamp. Biophys J. 2013;105:679-90 pubmed publisher
    ..A cytosolic protein, complexin (Cpx), binds to the SNARE bundle, and its accessory helix (AH) functions to clamp synaptic vesicle fusion...
  8. Cho R, Song Y, Littleton J. Comparative analysis of Drosophila and mammalian complexins as fusion clamps and facilitators of neurotransmitter release. Mol Cell Neurosci. 2010;45:389-97 pubmed publisher
    The SNARE-binding protein complexin (Cpx) has been demonstrated to regulate synaptic vesicle fusion...
  9. Rodal A, Motola Barnes R, Littleton J. Nervous wreck and Cdc42 cooperate to regulate endocytic actin assembly during synaptic growth. J Neurosci. 2008;28:8316-25 pubmed publisher
    ..Together, our results suggest that synaptic growth activated by growth factor signaling is controlled at an endosomal compartment via coordinated Nwk and Cdc42-dependent actin assembly. ..

More Information


  1. Lowe N, Rees J, Roote J, Ryder E, Armean I, Johnson G, et al. Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library. Development. 2014;141:3994-4005 pubmed publisher
    ..Our resource substantially increases the number of available protein traps in Drosophila and identifies new markers for cellular organelles and structures. ..
  2. Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon G, et al. Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes. Genome Res. 2007;17:1865-79 pubmed
    ..For mir-10 in particular, both arms show abundant processing, and both show highly conserved target sites in Hox genes, suggesting a possible cooperation of the two arms, and their role as a master Hox regulator. ..
  3. Pabst S, Hazzard J, Antonin W, Sudhof T, Jahn R, Rizo J, et al. Selective interaction of complexin with the neuronal SNARE complex. Determination of the binding regions. J Biol Chem. 2000;275:19808-18 pubmed
    ..Using purified proteins, we have performed a detailed analysis of the structure of complexin and of its interaction with SNARE proteins...
  4. Vasin A, Volfson D, Littleton J, Bykhovskaia M. Interaction of the Complexin Accessory Helix with Synaptobrevin Regulates Spontaneous Fusion. Biophys J. 2016;111:1954-1964 pubmed publisher
    ..A cytosolic protein, Complexin (Cpx), binds the SNARE complex and restricts spontaneous exocytosis by acting as a fusion clamp...
  5. Buhl L, Jorquera R, Akbergenova Y, Huntwork Rodriguez S, Volfson D, Littleton J. Differential regulation of evoked and spontaneous neurotransmitter release by C-terminal modifications of complexin. Mol Cell Neurosci. 2013;52:161-72 pubmed publisher
    ..Here, we demonstrate that the C-terminus of Drosophila complexin (DmCpx) regulates localization to synapses and that alternative splicing of the C-terminus can differentially ..
  6. Xue M, Lin Y, Pan H, Reim K, Deng H, Bellen H, et al. Tilting the balance between facilitatory and inhibitory functions of mammalian and Drosophila Complexins orchestrates synaptic vesicle exocytosis. Neuron. 2009;64:367-80 pubmed publisher
    ..Drosophila cause apparently opposite effects on neurotransmitter release, leading to contradictory hypotheses of Complexin function. Reconstitution experiments with different fusion assays and Complexins also yield conflicting results...
  7. Barber C, Jorquera R, Melom J, Littleton J. Postsynaptic regulation of synaptic plasticity by synaptotagmin 4 requires both C2 domains. J Cell Biol. 2009;187:295-310 pubmed publisher
    ..These data suggest that Syt 4 regulates activity-dependent release of postsynaptic retrograde signals that promote synaptic plasticity, similar to the role of Syt 1 as a Ca(2+) sensor for presynaptic vesicle fusion. ..
  8. Choi B, Imlach W, Jiao W, Wolfram V, Wu Y, Grbic M, et al. Miniature neurotransmission regulates Drosophila synaptic structural maturation. Neuron. 2014;82:618-34 pubmed publisher
    ..Our results establish that miniature neurotransmission, a universal but often-overlooked feature of synapses, has unique and essential functions in vivo. ..
  9. Cho R, Kümmel D, Li F, Baguley S, Coleman J, Rothman J, et al. Genetic analysis of the Complexin trans-clamping model for cross-linking SNARE complexes in vivo. Proc Natl Acad Sci U S A. 2014;111:10317-22 pubmed publisher
    b>Complexin (Cpx) is a SNARE-binding protein that regulates neurotransmission by clamping spontaneous synaptic vesicle fusion in the absence of Ca(2+) influx while promoting evoked release in response to an action potential...
  10. Sabeva N, Cho R, Vasin A, González A, Littleton J, Bykhovskaia M. Complexin Mutants Reveal Partial Segregation between Recycling Pathways That Drive Evoked and Spontaneous Neurotransmission. J Neurosci. 2017;37:383-396 pubmed publisher
    ..Genetic deletion of the SNARE-binding protein complexin dramatically increases spontaneous fusion, with the protein serving as the synaptic vesicle fusion clamp at ..
  11. Rieder L, Savva Y, Reyna M, Chang Y, Dorsky J, Rezaei A, et al. Dynamic response of RNA editing to temperature in Drosophila. BMC Biol. 2015;13:1 pubmed publisher
    ..Environmental cues, in this case temperature, rapidly reprogram the Drosophila transcriptome through RNA editing, presumably resulting in altered proteomic ratios of edited and unedited proteins. ..
  12. Uytterhoeven V, Lauwers E, Maes I, Miskiewicz K, Melo M, Swerts J, et al. Hsc70-4 Deforms Membranes to Promote Synaptic Protein Turnover by Endosomal Microautophagy. Neuron. 2015;88:735-48 pubmed publisher